Bone stromal cells. Medical search. Frequent questions

Bone marrow stem cells, including the pluripotent hematopoietic stem cells (HSCs) and bone mesenchymal stem cells (BMSCs), are being considered as potential targets for cell and gene therapy-based approaches against a variety of different diseases. (hindawi.com)
In this study, we investigated the modulation of pDC differentiation in Flt-3 ligand (Flt3L)-supplemented BM cultures, using highly purified mesenchymal stem cells (MSCs) that were FACS-isolated from murine BM based on surface marker expression and used after in vitro expansion. (nih.gov)
Recently, mesenchymal stem cells (MSCs) have been suggested for use in the cell-based treatment of cartilage lesions. (researchgate.net)
The emphasis was in the use of adult-derived mesenchymal stem cells as transplants to augment host tissue survival and improve functional recovery following SCI. (drexel.edu)
The aim of this study was to compare the osteogenic potential of mesenchymal stem cells obtained from bone marrow (BM-MSC) with those extracted from adipose tissue (AT-MSC) of adult dogs. (scielo.br)
Undifferentiated mesenchymal stem cells are pluripotent cells that can differentiate into various phenotypes and are a source of osteogenic Cells 1,2 . (bvsalud.org)

Three-dimensional (3D) spheroid culture can promote the osteogenic differentiation of bone marrow mesenchymal stromal cells (BMSC). (biomedcentral.com)
Moreover, proliferating cell nuclear antigen (PCNA) and CD31 showed co-localization with α-SMA, suggesting the differentiation of hBMSCs into epithelial cells and myofibroblasts/fibroblasts. (ijbs.com)
In order to eliminate the interference from the transplanted cells themselves, fetal stem cells were used for transplantation because the proliferation and differentiation potentials of fetal stem cells are superior to the adult ones ( 8 ). (ijbs.com)
Some of the main issues are identification of the optimal precursor cell types, establishment of growth and differentiation conditions that meet safety and good manufacturing practice standards, and manipulation of the surrounding environment to allow transplanted cells to survive and function. (sahmri.org.au)
When all three BAFF receptors are stimulated, levels of NF kappa B, which contributes to cell proliferation and differentiation, are increased in the nucleus. (wikipedia.org)
Six years later, research showing the key role of BLyS in B cell differentiation, survival, and activation was published. (wikipedia.org)
In this work, for the first time, we adopt a multidisciplinary approach to characterize the morphological, biochemical, and molecular changes underlying the response of human bone marrow stromal cells to long-term simulated microgravity exposure during osteogenic differentiation. (lu.se)
Despite their crucial role as a link between innate and adaptive immunity, little is known about the modulation of pDC differentiation by other bone marrow (BM) cells. (nih.gov)
Donor variation among patients, cell heterogeneity, and unpredictable capacity for differentiation reduce effectiveness of BMSCs for regenerative cell therapies. (elsevierpure.com)
The cell surface glycoprotein CD24 exhibits the most prominent differential expression during osteogenic versus adipogenic differentiation of human BMSCs. (elsevierpure.com)
In undifferentiated human BMSCs, CD24 cell surface expression is variable among donors (range: 2%-10%) and increased by two to fourfold upon osteogenic differentiation. (elsevierpure.com)
Strikingly, FACS sorted CD24pos cells exhibit delayed mineralization and reduced capacity for adipocyte differentiation. (elsevierpure.com)
The distribution and differentiation of the transplanted cells were subsequently evaluated with immunohistochemistry. (korea.ac.kr)
Our findings demonstrate the unique biological characteristics of e-MSCs as hematopoiesis-regulatory stromal cells at differentiation stage between MSCs and osteoprogenitor cells and have significant implications in developing new strategy for using pharmacological osteoinductive treatment to support hematopoiesis in hematopoietic stem and progenitor cell transplantation. (elsevierpure.com)
Presentation] Macrophage-secreted Emilin2 Stimulates Chemotaxis and Differentiation in Stromal/Osteoblastic Cells. (nii.ac.jp)
The cells were phenotypically categorized according to the expression of CD29, CD90, CD34 and CD45, and submitted to adipogenic and chondrogenic differentiation for 21 days and osteogenic differentiation for 7, 14 and 21 days. (scielo.br)
On days 7, 14 and 21 of osteogenic differentiation, the cultures were submitted to evaluations of MTT conversion in formazan, of alkaline phosphatase activity (AP), of collagen and mineralized matrix synthesis, evaluation of the number of cells per field and there was quantification of the gene transcripts for osterix, bone sialoprotein (BSP), osteonectin (ON) and osteocalcin (OC). (scielo.br)
The number of cells per field, the AP activity, the collagen and mineralized matrix synthesis were higher in AT-MSC en differentiation, in relation to BM-MSC under the same conditions in all evaluated times. (scielo.br)
Mutations in the ANKH gene that cause autosomal dominant craniometaphyseal dysplasia impair the maturation (differentiation) of osteoclasts, which likely disrupts bone remodeling. (medlineplus.gov)
Conclusions: This study showed that an osteoblastic cell lineage may be obtained from human bone marrow derived from adherent cells, and that the presence of the rhBMP-4 seems to have an effect during the first stages of differentiation only. (bvsalud.org)
Temporal multimodal single-cell profiling of native hematopoiesis illuminates altered differentiation trajectories with age. (lu.se)

Adipose tissue (AT) represents a commonly used source of mesenchymal stem/stromal cells (MSCs) whose proregenerative potential has been widely investigated in multiple clinical trials worldwide. (mdpi.com)
Here, by using a microarray analysis, we demonstrate that human BM mesenchymal stromal/stem cells (MSCs) in an early osteoinductive stage (e-MSCs) are characterized by unique hematopoiesis-associated gene expression with an enhanced hematopoiesis-supportive ability. (elsevierpure.com)
In comparison to BM-MSCs without osteoinductive treatment, gene expression in e-MSCs was significantly altered in terms of their cell adhesion- and chemotaxis-related profiles, as identified with Gene Ontology and Gene Set Enrichment Analysis. (elsevierpure.com)
Noteworthy, expression of the hematopoiesis-associated molecules CXCL12 and vascular cell adhesion molecule 1 was remarkably decreased in e-MSCs. (elsevierpure.com)
e-MSCs supported an enhanced expansion of CD34 + hematopoietic stem and progenitor cells, and generation of myeloid lineage cells in vitro. (elsevierpure.com)
e-MSCs exhibited the absence of decreased stemness-associated gene expression, increased osteogenesis-associated gene expression, and apparent mineralization, thus maintaining the ability to differentiate into adipogenic cells. (elsevierpure.com)
In this study, we aimed to clarify whether combination therapy of intracerebral transplantation of human modified bone marrow-derived MSCs, SB623 cells, and voluntary exercise with running wheel (RW) could exert synergistic therapeutic effects on a rat model of ischemic stroke. (biomedcentral.com)
SB623 cells are human modified bone marrow-derived MSCs transiently transfected with the human Notch-1 intracellular domain. (biomedcentral.com)
In vitro cell culture and animal studies have demonstrated that mesenchymal stromal cells (MSCs) have the capacity to modify immune responses and to enhance tissue repair. (bmj.com)
Mesenchymal stromal cells (MSCs) are cells of non-haematopoietic origin, with the capacity to differentiate into multiple lineages of the mesenchyme, that is, chondrocytes, osteoblasts and adipocytes. (bmj.com)
1 Unique MSC-specific markers have not yet been identified, and MSCs constitute a heterogeneous cell population, including both multipotent (stem) cells and progenitor cells and might even contain pluripotent cell fractions. (bmj.com)
2 MSCs were first described in the bone marrow where they constitute a small fraction of cells (0.001%-0.01%) that closely interact with haematopoietic cells to support haematopoiesis and skeletal homeostasis. (bmj.com)
3 4 Since then, it has become evident that MSCs reside in many tissues, including mesenchymal tissues (bone, adipose tissue, connective tissue), umbilical cord and several organs including the liver, spleen and lung. (bmj.com)
Following early observations that MSCs inhibit T-cell proliferation, 9 MSCs were found to interact with the majority of innate and adaptive immune cells. (bmj.com)
We have also developed grafting of exogenous, bone marrow-derived MSCs as a local, targeted therapy of GBM. (lu.se)

We report here that Ptch +/- mice develop primordial follicular neoplasms resembling human trichoblastomas, and that exposure to ultraviolet radiation or ionizing radiation results in an increase in the number and size of these tumors and a shift in their histologic features so that they more closely resemble human basal cell carcinoma. (nature.com)
The mouse basal cell carcinomas and trichoblastoma-like tumors resemble human basal cell carcinomas in their loss of normal hemidesmosomal components, presence of p53 mutations, frequent loss of the normal remaining Ptch allele, and activation of hedgehog target gene transcription. (nature.com)
The Ptch mutant mice provide the first mouse model, to our knowledge, of ultraviolet and ionizing radiation-induced basal cell carcinoma-like tumors, and also demonstrate that Ptch inactivation and hedgehog target gene activation are essential for basal cell carcinoma tumorigenesis. (nature.com)
Sex cord-stromal tumors are groups of tumors composed of granulosa cells, theca cells, Sertoli cells, Leydig cells, and fibroblasts of stromal origin, singly or in various combinations. (medscape.com)
[ 1 ] sex cord-stromal tumors are classified into the following categories. (medscape.com)
Sex cord-stromal tumors account for approximately 8% of all ovarian tumors. (medscape.com)
Although various markers have been reported to stain sex cord-stromal tumors (eg, CD99, CD56, A103, müllerian inhibiting factor, vimentin), inhibin and calretinin have proven to be the most helpful to date. (medscape.com)
CD56 is a sensitive marker of ovarian sex cord-stromal tumors and may also be useful in the diagnosis of this group of neoplasms, especially in cases that are inhibin or calretinin negative, and the differential diagnosis includes neoplasms that are CD56 negative. (medscape.com)
A worldwide database analysis revealed that younger age, smaller tumor size, early stage, and granulosa cell tumor histologic type appear to be independent prognostic factors for improved survival in patients with malignant sex cord-stromal tumors who undergo lymph node dissection. (medscape.com)
Tissue from both primary surgery and reoperations from the same patients, allows in depth analysis of the evolution of glioblastoma over time with the goal of development of diagnostic monitoring and identification of druggable GBM stromal targets in recurrent brain tumors. (lu.se)
Resolving the Pathogenesis of Anaplastic Wilms Tumors through Spatial Mapping of Cancer Cell Evolution. (lu.se)

Bone marrow stromal cells (BMSC) are multipotent stem cells and are considered good candidates for cell restoration in injured brain tissue. (sld.cu)
Bone marrow stromal cells (BMSC) are adult stem cells with characteristics that make them an attractive candidate in cellular therapy for neurological diseases (1). (sld.cu)
The aim of this study was to compare the potential of human BMSC cultured as 2D monolayers or 3D spheroids encapsulated in constructs of 3D-printed poly-L-lactide-co-trimethylene carbonate scaffolds and modified human platelet lysate hydrogels (PLATMC-HPLG) for bone regeneration. (biomedcentral.com)
Here, we investigate the function of MM-derived MIF in the MM microenvironment by examining its effects on bone marrow stromal cells (BMSC). (biomedcentral.com)
To investigate the effects of MIF secretion by MM on its microenvironment, we used cytokine arrays to establish if cytokine changes occur when MM cells are cultured with primary BMSC. (biomedcentral.com)
Recent experimental studies have shown that bone marrow stromal cells (BMSC) differentiate into neural cells and reduce neurological deficits when transplanted into traumatized spinal cord. (korea.ac.kr)
In all three rats subjected to SCI, green fluorescence was shown to spread from the site of BMSC-GFP injection toward the injury site, suggesting that the transplanted cells had migrated toward the lesion within the 4-week post-transplantation period. (korea.ac.kr)
3D three-dimensional, BMSC bone marrow-derived stromal cell, CCR chemokine (C motif) receptor, GPCR G protein-coupled receptor, MCP-1 monocyte chemoattractant protein 1, PI3K PI3 kinase. (figshare.com)

abstract = "The cloning of established human leukemia and lymphoma cell lines at limiting dilutions in microwells and soft agar is described. (edu.au)
abstract = "Replication of the Friend spleen focus forming virus (SFFV) in C3H/HeJ or C57BL/6J mouse continuous bone marrow cultures is associated with an increased cumulative production of pluripotential/hematopoietic stem cells (CFUs), granulocyte-macrophage progenitor cells (GM-CFUc), and total granulocytes, compared to uninfected or helper virus infected control cultures. (johnshopkins.edu)

The stromal cell requirement for androgen-mediated effects was confirmed in vivo by experiments using chimeric animals created by bone marrow transplantation in which androgen receptor expression was restricted to either the stromal or lymphoid cells of the bone marrow. (jci.org)
Studies have increasingly focused on the potential therapeutic effects of stem cell transplantation for neurological diseases [ 10 ]. (hindawi.com)
this limitation has been overcome via ectopic expression of human telomerase reverse transcriptase (hTERT), the catalytic component of telomerase, to produce large quantities of these cells as an attractive source for cellular transplantation [ 16 - 18 ]. (hindawi.com)
Traditional cell delivery methods involve direct seeding and attachment of MSC on biomaterial scaffolds before in vivo transplantation [ 26 ]. (biomedcentral.com)
Hu G, Liu P, Feng J, Jin Y. Transplantation with Bone Marrow Stromal Cells Promotes Wound Healing Under Chemotherapy through Altering Phenotypes. (ijbs.com)
Stem cell transplantation is a promising strategy for delayed wound healing caused by chemotherapy. (ijbs.com)
Nevertheless, myelosuppression can be reversed by autologous stem cell transplantation (SCT), even without additional toxicity ( 4 , 5 ). (ijbs.com)
While bone-marrow transplantation might mitigate hematopoietic syndrome, currently there are no approved medical countermeasures to alleviate radiation-induced gastrointestinal syndrome (RIGS), resulting from direct cytocidal effects on intestinal stem cells (ISC) and crypt stromal cells. (elsevierpure.com)
We examined whether bone marrow-derived adherent stromal cell transplantation (BMSCT) could restitute irradiated intestinal stem cells niche and mitigate radiation-induced gastrointestinal syndrome. (elsevierpure.com)
Methodology/Principal Findings: Autologous bone marrow was cultured in mesenchymal basal medium and adherent cells were harvested for transplantation to C57Bl6 mice, 24 and 72 hours after lethal whole body irradiation (10.4 Gy) or abdominal irradiation (16-20 Gy) in a single fraction. (elsevierpure.com)
Conclusion/Significance: Mitigation of lethal intestinal injury, following high doses of irradiation, can be achieved by intravenous transplantation of marrow-derived stromal cells, including mesenchymal, endothelial and macrophage cell population. (elsevierpure.com)
Among 1462 patients who had allogeneic hematopoietic cell transplantation (HCT) between January 2000 and December 2005, 116 (7.9%) developed stage 3-4 gut GVHD. (nature.com)
An acute graft-versus-host disease activity index to predict survival after hematopoietic cell transplantation with myeloablative conditioning regimens. (nature.com)
Reduced mortality after allogeneic hematopoietic-cell transplantation. (nature.com)
Prospective evaluation for upper gastrointestinal tract acute graft-versus-host disease after hematopoietic stem cell transplantation. (nature.com)
Such noninvasive bioimaging techniques would be valuable for monitoring the fate of these transplanted cells and assessing the safety and efficacy of their transplantation. (korea.ac.kr)
Mesenchymal stromal cell (MSC) transplantation therapy is a promising therapy for stroke patients. (biomedcentral.com)
This study suggests that combination therapy of intracerebral transplantation SB623 cells and voluntary exercise with RW achieves robust neurological recovery and synergistically promotes endogenous neurogenesis and angiogenesis after cerebral ischemia, possibly through a mechanism involving the up-regulation of BDNF and VEGF. (biomedcentral.com)
Cell transplantation therapy has emerged as a potential candidate treatment for ischemic stroke. (biomedcentral.com)
I have expertise in novel therapies for acute myeloid leukemia, stem cell mobilization and homing, as well as stem cell transplantation. (rochester.edu)

Bone marrow (BM) microenvironment has a crucial role in supporting hematopoiesis. (elsevierpure.com)
1: In the haematopoietic microenvironment, bone marrow stromal cells play an important role in regulating haematopoiesis by expressing various cytokines, including leukaemia inhibitory factor (LIF) and interleukin-6 (IL-6). (unboundmedicine.com)
Cancer cells, including leukemia cells, reside in a complex microenvironment, which influences biology and activity of the cells. (intechopen.com)
The present chapter outlines the importance of modern, multiparameter flow cytometry methods, which allow to analyze interactions between different types of cells within the leukemia microenvironment. (intechopen.com)
Influence of Bone Substitutes on Mesenchymal Stromal Cells in an Inflammatory Microenvironment. (bvsalud.org)

These results provide support for future pharmacological and clinical applications of SATB2, which accelerates bone regeneration around titanium implants. (nih.gov)
But the cartilage regeneration capacity of these cells remains unpredictable because of cell heterogeneity. (whiterose.ac.uk)
Since the iliac crest remains a frequent site of BM harvest for musculoskeletal regeneration, the authors also compared the spatial distribution of these subsets in trabeculae of femoral head and iliac crest and found CD2711CD561 bone-lining cells in both tissues. (whiterose.ac.uk)
Bone regeneration was assessed via in vivo computed tomography (CT), ex vivo micro-CT and histology. (biomedcentral.com)
The bone-regeneration kit relies on a collagen platform seeded with particles containing the genes needed for producing bone. (sciencedaily.com)
We delivered the DNA to the cells, so that the cells produce the protein and that's how the protein is generated to enhance bone regeneration," explains Aliasger Salem, professor in the College of Pharmacy and a co-corresponding author on the paper, published in the journal Biomaterials . (sciencedaily.com)
When cells migrate into the scaffold, they meet with the plasmid, they take up the plasmid, and they get the encoding to start producing PDGF-B, which enhances bone regeneration. (sciencedaily.com)

IHC staining analysis revealed that SATB2-overexpressing BMSCs were intensely distributed in the bone tissue surrounding the implant. (nih.gov)
However, obtaining primary neuronal cells from adult tissue is difficult and faces major ethical issues in clinical practice. (hindawi.com)
Background: Connective tissue progenitors (CTPs) from native bone marrow (BM) or their culture-expanded progeny, often referred to as mesenchymal stem/stromal cells, represents a promising strategy for treatment of cartilage injuries. (whiterose.ac.uk)
Regardless of monolayer or spheroid cell culture, PLATMC-HPLG constructs represent promising scaffolds for bone tissue engineering applications. (biomedcentral.com)
Bone tissue engineering (BTE) strategies are increasingly being used to overcome the limitations of autogenous bone grafts and existing bone-substitute materials to reconstruct such defects [ 1 ]. (biomedcentral.com)
Whilst it is clear that BMSSC-mediated osteogenesis has the capacity to integrate with pre-existing bone tissue, substantial progress in this and other approaches needs to be achieved for the accurate reassembly of functional complex organ systems. (sahmri.org.au)
It is anticipated that a concerted multidiscipline approach is required to facilitate the reality and great potential benefits of cell-based tissue engineering applications. (sahmri.org.au)
The 3D-perfusion system generated a stromal tissue that could be enzymatically treated to yield CD45- MSC. (unibas.ch)
Our data reveal that Compact disc133 appearance recognizes a indigenous human being bone tissue marrow come/progenitor cell that provides rise to BMSCs able of developing the HME. (opioid-receptors.com)
Bone marrow-derived mesenchymal stem/stromal cells (BMSCs) are fundamental to bone regenerative therapies, tissue engineering, and postmenopausal osteoporosis. (elsevierpure.com)
Functional specialization among BMSCs populations may support their regenerative potential and therapeutic success by accommodating cell activities that promote skeletal tissue formation, homeostasis, and repair. (elsevierpure.com)
At day 1 or day 21 post-operative, defects were analyzed by micro-computed tomography, histomorphometry and stereology for bone and soft tissue repair. (polymtl.ca)
All implants attracted neutrophils, osteoclasts and abundant bone marrow-derived stromal cells, stimulated bone resorption followed by new woven bone repair (bone remodeling) and promoted repair tissue-bone integration. (polymtl.ca)
Osteonecrosis of the femoral head (ONFH) is defined as a tissue disorder and successive subchondral bone collapse resulting from an ischemic process, which may progress to hip osteoarthritis. (upc.edu)
The plasmid-seeded bio patch grew 44-times more bone and soft tissue in the affected area than with the scaffold alone, and was 14-fold higher than the affected area with no manipulation. (sciencedaily.com)
Expert Alert: Proton beam therapy for bone cancer spares surrounding tissue July 13, 2022, 04:00 p.m. (mayoclinic.org)
The effects of adult-derived stem cells in the repair of SCI would be particularly interesting because such cells are readily available and could be generated from the individuals own tissue, avoiding the problem of graft rejection of foreign tissue by the host. (drexel.edu)
Reduced breakdown of bone tissue can contribute to the bone thickening characteristic of craniometaphyseal dysplasia. (medlineplus.gov)
The applicant is responsible for a brain tumor tissue biobank which provides the basis for the studies on human GBM tumor cells and stroma. (lu.se)

While adult lymphoid progenitor cells are produced by the bone marrow, their lineage commitment and development into mature T-cells is dependent on their migration into the thymus, where essential interactions with heterogeneous thymic stromal cells take place ( 1 ). (frontiersin.org)

Cultured adherent bone marrow stromal cells (BMSCs) are capable of forming ectopic hematopoietic microenvironments (HMEs) in immunodeficient mice. (opioid-receptors.com)
this cell can be most likely to provide rise to cultured adherent BMSCs. (opioid-receptors.com)
To determine whether viral effect(s) occurred in adherent stromal and/or non-adherent hematopoietic stem cells, purified cell populations, comprised exclusively of cells from each compartment, were separated from C57BL/6J (Fv-1 bb ) or C3H/HeJ (Fv-1 nn ) marrow cultures and were left uninfected or were infected with host range replication restricted B-tropic or N-tropic helper pseudotype viruses of SFFV respectively. (johnshopkins.edu)
Thus, these data indicate that the prolongation of hematopoiesis in undisturbed long-term marrow cultures by SFFV occurs through an interaction of adherent hematopoietic stem cells with the marrow stroma. (johnshopkins.edu)
MyeloCult™ media have been developed for the long-term culture of hematopoietic progenitor cells in association with adherent stromal cells. (stemcell.com)
Results: Bone marrow cells were adherent since the first day of culture and were positive for osteonectin. (bvsalud.org)

In systemic lupus erythematosus patients, BAFF is overexpressed, which may cause autoimmune B cell proliferation and survival. (wikipedia.org)
medical citation needed] Another B-cell activator similar to BAFF is APRIL (A proliferation-inducing ligand), but APRIL activates only BCMA and TACI, not BAFF-R.[medical citation needed] Belimumab reduces the number of circulating B cells, but anti-CD20 monoclonal antibodies reduce the number even more. (wikipedia.org)
Downregulated genes are associated with cell cycle regulation, and biological assays revealed that CD24pos cells have reduced proliferation. (elsevierpure.com)
Hence, expression of the cell surface glycoprotein CD24 identifies a subpopulation of human BMSCs with reduced capacity for proliferation and extracellular matrix mineralization. (elsevierpure.com)
IM combined with IR can synergistically inhibit the proliferation, apoptosis, clonogenesis, invasion, migration and cell cycle of GIST cells. (researchsquare.com)
We have already acquired proof-of-principle showing that reprogramming towards astrocytic fate causes morphological changes, reduces proliferation and bestow astrocytic function in human GBM cell lines. (lu.se)

We employed in vitro cultures and two in vivo models to investigate whether androgens exert these effects directly on marrow lymphoid precursors or whether actions on marrow stromal elements are required. (jci.org)
Following in vitro exposure to CTX, hBMSCs showed decreased cell growth in a dose- and time-dependent manner, accompanied by increased expressions of collagen-I/III, and CD31. (ijbs.com)
In this review, we discuss the rationale for MSC-based cell therapy in COPD, the main findings from in vitro and in vivo preclinical COPD model studies, clinical trials in patients with COPD and directions for further research. (bmj.com)
In vitro effects of benzene metabolites on mouse bone marrow stromal cells. (cdc.gov)
Cells derived from bone marrow can undergo osteoinduction in vitro in the absence of osteoinductive factors such as bone morphogenetic proteins. (bvsalud.org)
As a third major line of research, we explore, in collaboration with associate professor Henrik Ahlénius group if GBM cells can be reprogrammed to neuron like cells in vitro and in vivo. (lu.se)

c Representative ( n = 3) Human XL cytokine array output after a 24-h incubation in either mono or co-culture, cell supernatant was used for analysis. (biomedcentral.com)
In addition to intrathymic selection, and during this medullary residency period, CD4 + and CD8 + single positive (CD4SP, CD8SP) thymocytes acquire the ability to proliferate in response to TCR stimulation, and undergo licensing for cytokine production, prior to exiting the thymus to join the peripheral T-cell pool as Recent Thymus Emigrants (RTE) ( 5 , 6 ). (frontiersin.org)
However, the intracellular signal that regulates cytokine secretion in bone marrow stromal cells has not been determined. (unboundmedicine.com)
Effects of hematopoietic stem cell adhesion on marrow stromal cell cytokine p. (rochester.edu)

Herein we characterized human fetal bone marrow stromal cells (hBMSCs) during wound healing in mice treated with cyclophosphamide (CTX). (ijbs.com)

Control constructs seeded with human dermal fibroblasts formed connective cells, but failed to form HMEs. (opioid-receptors.com)

Although human HSCs as vehicles to treat metachromatic leukodystrophy (MLD) has been used to treat patients with early onset MLD in a phase I/II trial, the HSCs give rise to all different blood cell lineages, such as the myeloid and lymphoid cell lineages [ 11 ]. (hindawi.com)
B cells develop in the bone marrow and continue to mature peripherally in secondary lymphoid organs and in the gut. (wikipedia.org)
While many studies have demonstrated the importance of cTEC and mTEC during T-cell development, thymic stromal microenvironments also contain heterogenous non-epithelial stromal (NES) populations in a similar manner to that seen in peripheral lymphoid tissues. (frontiersin.org)

Immature B cells from normal mouse bone marrow were not responsive to the suppressive effect of androgens unless they were cocultured with marrow stromal cells or with supernatants from androgen-treated stromal cells, suggesting that the androgen effects are exerted through marrow stromal elements by production of a diffusible mediator. (jci.org)

When autoimmune B cells attack the body's own tissues, they are normally destroyed by cell suicide (apoptosis). (wikipedia.org)
Because bone marrow-derived stromal cells (BMSCs) are able to generate many cell types, they are envisioned as source of regenerative cells to repair numerous tissues, including bone, cartilage, and ligaments. (upc.edu)
This gene provides instructions for making a protein called connexin 43, which is involved in the development of many tissues in the body, including bone. (medlineplus.gov)
Cells Tissues Organs. (cancercentrum.se)

Real-time qRT-PCR results showed that the local delivery of SATB2-overexpressing BMSCs enhanced expression levels of potent osteogenic transcription factors and bone matrix proteins in the implantation sites. (nih.gov)
Results: Cells from the BM after rasping were significantly more chondrogenic than the donor-matched aspirate, whereas no notable difference in their osteogenic or adipogenic potential was observed. (whiterose.ac.uk)
BTE aims to combine the cellular ( osteogenic cells), extracellular ( osteoconductive scaffolds) and/or molecular elements ( osteoinductive growth factors) required for bone healing [ 2 ]. (biomedcentral.com)
The investigation of cell proteome highlighted how simulated microgravity affects a relatively low number of proteins compared to time and/or osteogenic factors and has allowed us to reconstruct a hypothetical pipeline for cell response to simulated microgravity. (lu.se)
OBJECTIVE: To assess the efficacy of osteogenic protein-1 (OP-1) for long bone nonunion. (who.int)
Objective: This study evaluated the osteogenic induction of human bone marrow cells by human recombinant bone morphogenetic protein-4 (rhBMP-4) and proteins released by Saos-2 (human osteosarcoma cell line). (bvsalud.org)

Immuno-electron microscopy characterization of human bone marrow stromal cells with anti-NGFR antibodies. (atsbio.com)
Caneva L, Soligo D, Cattoretti G, De Harven E, Deliliers GL (1995) Immuno-electron microscopy characterization of human bone marrow stromal cells with anti-NGFR antibodies. (atsbio.com)
Bone marrow stromal cells: characterization and clinical application. (bvsalud.org)

In vivo CT revealed comparable bone formation after 4, 8 and 12 weeks in all groups. (biomedcentral.com)
However, this method may not be optimal for the delivery of cell spheroids where the 3D structure, essential to maximize their in vivo effects, is lost by direct seeding. (biomedcentral.com)
NDLI: In vivo efficacy of bone marrow stromal cells coated with beta-tricalcium phosphate for the reconstruction of orbital defects in canines. (iitkgp.ac.in)
The study is novel in that the researchers directly delivered bone-producing instructions (using piece of DNA that encodes for a platelet-derived growth factor called PDGF-B) to existing bone cells in vivo, allowing those cells to produce the proteins that led to more bone production. (sciencedaily.com)

These results provide evidence for Waif1 on stromal/osteoblastic cells as a receptor of osteoclast-secreted Cthrc1, and suggest that Waif1 serves an important coupling function of bone resorption to formation. (nii.ac.jp)

Clinical Relevance: The harvest technique of BM may be of major importance in determining the clinical success of BM mesenchymal stem/stromal cells in cartilage repair. (whiterose.ac.uk)
Background: In this study we evaluated a novel approach to guide the bone marrow-driven articular cartilage repair response in skeletally aged rabbits. (polymtl.ca)
Pre-solidified chitosan implants are tunable by molecular mass, and could be beneficial for augmented marrow stimulation therapy if the recruited stromal cells can progress to bone and cartilage repair. (polymtl.ca)

The construction of this cell line is the first promising step in the regulation of GAL secretion from hTERT-immortalized BMSCs, and the potential application of this system may provide a stem cell-based research platform for pain. (hindawi.com)
Indeed, the potent pathotropic migratory properties of BMSCs and ability to circumvent both the complications associated with immune rejection of allogenic cells and many of the moral reasons associated with embryonic stem cell use suggest that BMSCs are most promising stem cells as a potential target for the clinical use of genetically engineered stem cells [ 14 , 15 ]. (hindawi.com)
Hypothesis: The harvest technique of BM may highly influence stem cell heterogeneity and, thus, cartilage formation because these cells have distinct spatial localization within BM from the same bone. (whiterose.ac.uk)
Mesenchymal stromal/stem cell (MSC) expansion in conventional monolayer culture on plastic dishes (2D) leads to progressive loss of functionality and thus challenges fundamental studies on the physiology of skeletal progenitors, as well as translational applications for cellular therapy and molecular medicine. (unibas.ch)
Effects of oxygen metabolites/chemokines on hematopoietic stem cell expansion and preservation. (rochester.edu)

Arguably, the most recognized and well-studied stromal populations in thymus biology are thymic epithelial cells (TEC) within the cortical and medullary areas, which are defined by their anatomical separation and function. (frontiersin.org)
Thus, it is crucial to improve analytical methods of investigation of co-cultured cells, to study their interactions and so to understand biology of leukemia in order to understand molecular mechanisms and offer novel therapeutic strategies. (intechopen.com)

Significantly, there are additional non-epithelial stromal cells (NES) that exist alongside TEC within thymic microenvironments, including multiple subsets of mesenchymal and endothelial cells. (frontiersin.org)
Broadly separated into mesenchymal and endothelial cells, NES have been implicated in thymus organogenesis, thymocyte development, tolerance induction and development/maintenance of epithelial stroma. (frontiersin.org)
Some authorities suggest that the primary endothelial cell defect with secondary thrombosis leads to infarctive changes manifesting as Degos disease. (medscape.com)

This inhibition was cell contact-dependent and soluble factor-independent, as indicated by trans-well experiments. (nih.gov)

In this respect, different routes of interactions were defined, such as direct cell-cell interactions or indirect cross talk, by release of soluble factors or vesicular particles containing proteins, RNAs and other molecules. (intechopen.com)
CD117 by immunohistochemical methods sion molecules, involved in cell-cell and in order to clarify the role of the infiltrating cell matrix interactions and thought to take inflammatory cells in the pathomechanisms part in cell motility [ 2,3 ]. (who.int)

In this review, we cover how the non-epithelial compartment of thymic stroma represent essential cell populations in regulating thymus function. (frontiersin.org)
Remodeling of stroma cell functions by leukemia cells is also well documented. (intechopen.com)

Cortical epithelial cells (cTEC) support the earliest thymocyte progenitor populations, guiding them through the cortex, directing them to a T-cell lineage fate and ensuring their functional qualities as self-MHC restricted cells through the process of positive selection ( 2 ). (frontiersin.org)
Cell populations were then mixed to establish continuous hematopoiesis in allogeneic or syngeneic combinations. (johnshopkins.edu)

It interacts with three membrane receptors on B lymphocytes:[medical citation needed] BAFF-R (BAFF receptor) BCMA (B cell maturation antigen) TACI (transmembrane activator and calcium modulator and cyclophylin ligand interactor) When BAFF binds to BAFF-R and BCMA on B cells, levels of Bcl-2, a survival factor, are increased. (wikipedia.org)
The B-cell receptor consists of an Ig molecule anchored to the cell's surface. (msdmanuals.com)

better source needed] B-cell activating factor is a naturally occurring protein that was discovered by researchers from National Jewish Health (previously the National Jewish Medical and Research Center) and the University of Colorado, who jointly published a paper detailing their findings in May 1999 and named the protein TALL-1. (wikipedia.org)

2007) referred to the Compact disc146 epitope (Most cancers Cell Adhesion Molecule, MCAM) as an antigen associated with self-renewing human osteoprogenitors capable of HME formation when transplanted subcutaneously in hydroxyapatite/tri-calcium phosphate (HA/TCP)/fibrin constructs [11]. (opioid-receptors.com)
RNAseq analysis of CD24pos and CD24neg BMSCs identified a limited number of genes with increased expression in CD24pos cells that are associated with cell adhesion, motility, and extracellular matrix. (elsevierpure.com)
Little is known about molecular mecha- desmosomal glycoproteins, resulting in the nisms affecting mast cell and T lympho- loss of keratinocyte cell-cell adhesion [ 1 ]. (who.int)

This is attributable, in part, to the highly protective nature of the BM micro-environment niche in which the malignant plasma cells proliferate. (biomedcentral.com)
Generation BAY 61-3606 of HMEs by BMSCs transplanted to ectopic sites suggests that such cells retain cell-autonomous information necessary for niche formation. (opioid-receptors.com)

The use of human telomerase reverse transcriptase-immortalized bone marrow mesenchymal stromal cells (hTERT-BMSCs) as vehicles to deliver antinociceptive galanin (GAL) molecules into pain-processing centers represents a novel cell therapy strategy for pain management. (hindawi.com)
However, the standardization of the manufacturing process of MSC-based cell therapy medicinal products in compliance with the requirements of the local authorities is obligatory and will allow us to obtain the necessary permits for product administration according to its intended use. (mdpi.com)
Mesenchymal stromal cells: a novel therapy for the treatment of chronic obstructive pulmonary disease? (bmj.com)
Cell Therapy for Parkinsons Disease: What Next? (lu.se)

The pyrophosphate found outside of cells (extracellular pyrophosphate) helps control bone formation by preventing mineralization, the process by which minerals such as calcium and phosphorus are deposited in developing bones. (medlineplus.gov)
A shortage of extracellular pyrophosphate can increase bone mineralization, which may also contribute to the bone abnormalities. (medlineplus.gov)

Here we demonstrate that 2D MSC expansion can be entirely bypassed by culturing freshly isolated bone marrow nucleated cells within 3D porous scaffolds in a perfusion-based bioreactor system. (unibas.ch)

Reconstruction of advanced maxillofacial bone deficiencies is a clinical challenge. (biomedcentral.com)
This step is important not only to enhance the efficacy of MSC expansion but also to facilitate clinical translation of cell therapies according to current regulations [ 11 ]. (biomedcentral.com)

It functions by slowing or blocking cell growth and compromising the immune system's response. (ijbs.com)
Treatment for this condition remains difficult and largely ineffective, because the growth of cells in the wound is inhibited ( 2 ) and chemotherapy usually leads to myelosuppression, the latter of which further compromises the migration of multipotent cells from the bone marrow ( 3 ). (ijbs.com)
Growth of most cell lines was improved by the addition of human AB serum and irradiated human bone marrow stromal cells. (edu.au)
The effect of bone marrow stromal cells appeared to be caused by a diffusable factor(s), but close cellular interaction could not be excluded since cloning in microwells produced consistent and optimal cell growth compared to growth in soft agar. (edu.au)
The plasmids are absorbed by bone cells already in the body, spurring new growth. (sciencedaily.com)
It also stimulated new growth in human bone marrow stromal cells in lab experiments. (sciencedaily.com)
Aerial and cross-sectional scans showed the plasmid-encoded scaffolds had spurred enough new bone growth to nearly close the wound area, the researchers report. (sciencedaily.com)
Elangovan and Salem next hope to create a bio platform that promotes new blood vessel growth- needed for extended and sustained bone growth. (sciencedaily.com)
The abnormal bone growth continues throughout life. (medlineplus.gov)
In severe cases, abnormal bone growth can pinch (compress) the nerves that extend from the brain to various areas of the head and neck (cranial nerves). (medlineplus.gov)
As human osteosarcoma cells are a source of additional growth they did not affect osteoinduction. (bvsalud.org)
Osteoblasts were obtained from human bone marrow cells even in the absence of growth factors and showed a typical morphology. (bvsalud.org)

3D printing offers the possibility to produce customized scaffolds for complex bone defects. (biomedcentral.com)

Plasmacytoid dendritic cells (pDCs) are rare central regulators of antiviral immunity and unsurpassed producers of interferon-α (IFN-α). (nih.gov)
Medullary epithelial cells (mTEC), in conjunction with dendritic cells, then take over control of developing thymocytes. (frontiersin.org)

and salivary epithelial cells in Sjögren syndrome. (wikipedia.org)
Extensive research on thymic epithelial cells (TEC) within the cortex and medulla has defined their essential roles during T-cell development. (frontiersin.org)

C: Tumor either Stage IA or Stage IB, but with tumor on the surface of one or both ovaries, or with capsule ruptured, or with ascites containing malignant cells or with positive peritoneal washings. (medscape.com)

Transplanted BMSCs that form HMEs also presumably secrete chemokines such as stromal-derived factor 1 alpha (SDF-1) that attract host-derived CD34-positive vascular and hematopoietic progenitors that then initiate hematopoiesis [14]. (opioid-receptors.com)

Anatomy12

Organisms1

Diseases3

Chemicals and Drugs2

Analytical, Diagnostic and Therapeutic Techniques and Equipment4

Phenomena and Processes9

Disciplines and Occupations1

Mesenchymal Stem6

Differentiation21

MSCs15

Tumors11

BMSC8

Abstract2

Transplantation20

Microenvironment5

Regeneration7

Tissue19

Adult1

Adherent6

Proliferation6

Vitro6

Cytokine4

Human fetal1

Fibroblasts1

Lymphoid3

Mouse bone marrow1

Tissues4

Osteogenic6

Characterization3

Vivo4

Osteoblastic1

Cartilage repair3

Stem cell5

Biology2

Endothelial3

Inhibition1

Interactions2

Stroma2

Populations2

Receptor2

19991

Adhesion3

Niche2

Therapy4

Extracellular2

Isolated bone marrow1

Clinical2

Growth12

Scaffolds1

Dendritic Cells2

Epithelial cells2

Tumor1

Chemokines1