Body normal mice mice knockout. Medical search. Frequent questions

In this study, we investigated the effects of N-butyldeoxygalacto-nojirimycin (N B-DGJ), an imino sugar that inhibits ganglioside biosynthesis, in normal C57BL/6J mice and in beta-gal knockout (beta-gal-/-) mice from postnatal day 9 (p-9) to p-15. (wikigenes.org)
Methods: An obesity model was built by injecting 8-week-old C57BL/6 mice (n = 6 mice per group) with Ad.Null and Ad.HKS adenovirus into epididymal adipose tissue and fed with a high-fat diet (HFD). (bvsalud.org)

In mice, KMT2D is coded by the Kmt2d gene located on chromosome 15F1. (wikipedia.org)
In the hearts of mice, a single copy of the Kmt2d gene is sufficient for normal heart development. (wikipedia.org)
Scientists at The Scripps Research Institute (TSRI) have discovered that a gene mutation linked to hereditary spastic paraplegia, a disabling neurological disorder, interferes with the normal breakdown of triglyceride fat molecules in the brain. (news-medical.net)
To start, the researchers created a mouse model of DDHD2-related HSP, in which a targeted deletion from the DDHD2 gene eliminated the expression of the DDHD2 protein. (news-medical.net)
Cravatt's team compared the tissues of the no-DDHD2 mice to the tissues of mice with normal versions of the gene, and also found that the mutant mice had much higher levels of a type of fat molecule, principally in the brain. (news-medical.net)
Whole-body knockout of the Mig6 gene in mice has been shown to induce osteoarthritis and joint degeneration. (bepress.com)
To assess the role of an individual HDAC isoenzyme in physiology and tumor development, HDAC2-mutant mice were generated from a gene trap embryonic stem cell clone. (aacrjournals.org)
When compared with the human genome it was found that the two genomes were of similar size and almost every gene in the human genome has a counterpart in the mouse. (yourgenome.org)
Almost every gene in the human genome has a counterpart in the mouse. (yourgenome.org)
Transgenic mice are extremely useful for scientists studying gene function. (yourgenome.org)
LV weight was not significantly elevated in relation to other internal organs and gene expression of LVH markers was normal, suggesting an absence of hypertrophy. (ox.ac.uk)
The mouse testis-enriched Znf230 gene, which encodes a type of RING finger protein, is present primarily in the nuclei of spermatogonia, the acrosome and the tail of spermatozoa. (scielo.org)
Marker gene analysis showed that rnf141 may play a role in normal dorsoventral patterning of zebrafish embryos, suggesting that rnf141 may have a broad function during early development of vertebrates. (scielo.org)
In it, the researchers announce that the presence of the Bmal1 gene in the striatum affects alcohol consumption in both male and female mice - but in a sexually dimorphic manner. (concordia.ca)
The researchers created two lines of mice, using molecular biology methods to delete or "knock out" the Bmal1 gene from the striatum's medium spiny neurons in one of them. (concordia.ca)
The gene remained present in other parts of the body, since it plays a critical role in the circadian clock. (concordia.ca)
The sex differences you see in normal mice are eliminated when the gene is taken out of the striatum. (concordia.ca)
They identified a gene which was active in the egg cells but not in the surrounding ovary tissues - foxl3 - and sought to determine its purpose by creating knockout fish lineage that lacks expression of that gene. (discovermagazine.com)
They developed a 'knockout' mouse, in which the gene that codes for Abcc10 was missing, or knocked out. (news-medical.net)
The researchers isolated cells from the knockout mice and tested the cells' reactions to taxanes and two other anticancer drugs, vincristine and Ara-C. Compared to cells from normal mice that still possessed the gene for Abcc10, the knockout mouse cells were much more sensitive to the drugs. (news-medical.net)
A knockout mouse , or knock-out mouse , is a genetically modified mouse ( Mus musculus ) in which researchers have inactivated, or " knocked out ", an existing gene by replacing it or disrupting it with an artificial piece of DNA . (wikipedia.org)
By causing a specific gene to be inactive in the mouse, and observing any differences from normal behaviour or physiology, researchers can infer its probable function. (wikipedia.org)
Gene knockout in rats is much harder and has only been possible since 2003. (wikipedia.org)
A laboratory mouse in which a gene affecting hair growth has been knocked out (left) is shown next to a normal lab mouse. (wikipedia.org)
Consequently, observing the characteristics of knockout mice gives researchers information that can be used to better understand how a similar gene may cause or contribute to disease in humans. (wikipedia.org)
[3] Many mouse models are named after the gene that has been inactivated. (wikipedia.org)
For example, the p53 knockout mouse is named after the p53 gene which codes for a protein that normally suppresses the growth of tumours by arresting cell division and/or inducing apoptosis. (wikipedia.org)
White heterozygous mice can subsequently be crossed to produce mice that are homozygous for the knocked out gene. (wikipedia.org)
The gene to be knocked out is isolated from a mouse gene library . (wikipedia.org)
Usually, the new sequence is also given a marker gene , a gene that normal mice don't have and that confers resistance to a certain toxic agent (e.g., neomycin) or that produces an observable change (e.g. colour or fluorescence). (wikipedia.org)
Our initial pilot study demonstrated that MTMR14 knock out (KO) mice gain weight earlier than their wild-type (WT) littermates, which suggests that this gene may also be involved in metabolism regulation. (biomedcentral.com)

They have cultured mES cells to make homozygous mutant cells from heterozygous cells, to delete genes in targeted mES cells using Cre-recombinase, and to create embryoid bodies to promote differentiation of mES cells into multiple cell lineages. (ny.gov)
The homozygous Znf230-knockout (KO) mice did not exhibit Znf230 mRNA expression and Znf230 protein production. (scielo.org)
Results: Slc20a2 homozygous knockout (Slc20a2-HO) mice showed congenital and global developmental delay, lean body mass, skeletal malformation, and a high proportion of unilateral or bilateral eye defects. (ox.ac.uk)

In addition, mutation of Y5477 in mouse KMT2D, which corresponds to Y5426 in human KMT2D, resulted in the inactivation of KMT2D's enzymatic activity in embryonic stem cells. (wikipedia.org)
Whole-body knockout of Kmt2d in mice results in early embryonic lethality. (wikipedia.org)
A second method involves introducing the transgenic DNA into embryonic stem cells (ES cells) derived from a mouse embryo at the very early stages of development. (yourgenome.org)
The embryonic stem cells need a host embryo in which to develop and this is isolated usually from a mouse with different colour fur. (yourgenome.org)
Professor Gronostajski's lab uses mouse Embryonic Stem Cells (mES) to make mice with mutations in the genes for the Nuclear Factor I transcription factors. (ny.gov)
Embryonic stem cells are isolated from a mouse blastocyst (a very young embryo ) and grown in vitro . (wikipedia.org)

Using a set of sophisticated 'lipidomics' tests to analyze the accumulating fat molecules, they identified them as triglycerides-a major component of stored fat in the body, and a risk factor for obesity, atherosclerosis and type 2 diabetes. (news-medical.net)
Examples of research in which knockout mice have been useful include studying and modeling different kinds of cancer , obesity , heart disease , diabetes , arthritis , substance abuse , anxiety , aging and Parkinson's disease . (wikipedia.org)
We used MTMR14 KO male mice as a working model to investigate the mechanism of MTMR14 in obesity. (biomedcentral.com)
A series of physiological indexes demonstrated that the loss of MTMR14 induced obesity in an age-dependent manner, as reflected by body weight, energy intake and expenditure, blood biochemical indexes, and fat accumulation. (biomedcentral.com)

Male and female Tpcn2 knockout (KO), heterozygous (Het), and wild-type (WT) mice were fed a low-fat (LF) or high-fat (HF) diet for 24 wk. (ox.ac.uk)
Brain calcifications were detected in the hypothalamus, ventral thalamus, and midbrain early at postnatal day 80 in Slc20a2-HO mice, but were seldom found in Slc20a2 heterozygous knockout (Slc20a2-HE) mice, even at extremely old age. (ox.ac.uk)

The TSRI researchers found large droplets of triglycerides within the neurons of mice modeling the disease. (news-medical.net)
We were able to show as well, using both light microscopy and electron microscopy, that droplets of triglyceride-rich fat are present in the neurons of DDHD2-knockout mice, in several brain regions, but are not present in normal mice,' said Inloes. (news-medical.net)
Increase in c-Fos, phosphorylated extracellular signal-regulated kinase, and hyperexcitability of PVA neurons were detected in hyperalgesic mice. (iasp-pain.org)
We report here that the Fgf2 null mutant mice show an ∼40% decrease in cortical glutamatergic pyramidal neurons. (jneurosci.org)
Because Fgf2 −/− mice lacked 45% of cortical neurons at birth, the progenitor loss is compensated only in part during neurogenesis. (jneurosci.org)
Because the dorsal PVE was depleted of progenitors but the ganglionic eminences were not affected in Fgf2 −/− mice, we predicted that these mice should lack a population of pyramidal neurons, but their cortical GABA interneurons should have remained unchanged. (jneurosci.org)

Such stark differences in phenotype led the scientists to make a liver-specific PCTP knockout. (anl.gov)
Mice deficient in muscle- and mitochondrial-CK (M/Mt-CK(-/-)) have previously been described, but exhibit an unexpectedly mild phenotype of compensated left ventricular (LV) hypertrophy. (ox.ac.uk)
Results: CKO mice developed an obese phenotype, more severely under high-fat diet (HFD) feeding. (tmu.edu.tw)

The scientists first created a whole-body PCTP knockout male mouse (meaning one in which genes have been activated or deactivated), assessing its fat metabolism under different dietary conditions, including normal and high-fat diet. (anl.gov)
Consistent with the multiplex immunoassay data, DKO mice also displayed a significantly decreased level of mRNA corresponding to immune genes in response to WNV infection when compared to WT. (hawaii.edu)
He could then use these mice to investigate which genes were responsible for that charact. (yourgenome.org)
This allows scientists to investigate what specific genes do in the body. (yourgenome.org)
Gronostajski's lab is culturing mouse neural stem cells (NSC) to assess changes in NSC number in some of our NFI knockout mice and investigating the effect of loss of NFI genes on the differentiation of NSCs into neural and glial cells. (ny.gov)
A Female medaka lacking foxl3 genes, looking very much like a normal female medaka - but her ovaries (lower images) tell a different story. (discovermagazine.com)
Humans share many genes with mice. (wikipedia.org)

The researchers conducted a series of sophisticated tests in both living mouse brains and human cells to see if this chain of events was taking place. (parkinson.org)
As a result, researchers have been able to develop thousands of mouse strains with mutations that mirror those seen in human genetic disease . (yourgenome.org)
Notably, researchers speculated that neither lean mice nor genetically engineered mice (mice known as "beta(3)-AR knock-out mice") appeared to react to the lipolytic effects of AOD 9604 [vi]. (asianage.com)
Researchers first confirmed the distribution of SR-B1 and the uptake of rHDL nanoparticles in mice injected with cancer cells. (nanowerk.com)

If we can knock out 70, 80 or 90 percent of tumors without drug accumulation in normal tissues in mice, it is likely that many cancer patients could benefit from this new type of treatment in the long run," Lacko said. (nanowerk.com)
Several inflammatory cytokines and adipokines were dramatically dysregulated in the metabolic tissues of aged MTMR14 KO mice compared to control mice. (biomedcentral.com)
As we expected, MTMR14 mRNA and protein were almost undetectable in the metabolic tissues of male KO mice at different ages (Fig. 1 b, c). (biomedcentral.com)

Both pharmacologic blockade of the lung's ENaC channel and genetic knockout experiments using mice deficient in the ENaC pore-forming subunit have demonstrated the critical physiologic importance of lung Na+ transport at birth. (medscape.com)
Crossing of HDAC2-mutant with tumor-prone APC min mice revealed tumor rates that are lower in HDAC2-deficient mice by 10% to 100% depending on segment of the gut and sex of the mice. (aacrjournals.org)
To investigate the role of Znf230 in spermatogenesis, we generated Znf230-deficient mice by disrupting Znf230 exon-5 and exon-6 using homologous recombination. (scielo.org)
Slc20a2-Deficient Mice Exhibit Multisystem Abnormalities and Impaired Spatial Learning Memory and Sensorimotor Gating but Normal Motor Coordination Abilities. (ox.ac.uk)
We comprehensively evaluated brain calcifications and PFBC-related behavioral abnormalities in Slc20a2-deficient mice. (ox.ac.uk)

Bacterial strains within the body can directly interact with the mechanisms of disease through the production of natural antibiotics and preliminary immune signaling. (broadinstitute.org)
Now, there are over 100 different inbred strains of mice, each with a different genetic background. (yourgenome.org)
There are several thousand different strains of knockout mice. (wikipedia.org)

Transgenic mice are mice that contain additional, artificially introduced genetic material in every cell. (yourgenome.org)
Male M/Mt-CK(-/-) mice on the mixed genetic background developed congestive heart failure as evidenced by significantly elevated end-diastolic pressure, impaired contractility, LV dilatation, hypertrophy and pulmonary congestion. (ox.ac.uk)
They are widely used in knockout experiments, especially those investigating genetic questions that relate to human physiology . (wikipedia.org)

Mature newborns who have normal transitions from fetal to postnatal life have mature surfactant and lung epithelial systems. (medscape.com)
Substrate reduction reduces gangliosides in postnatal cerebrum-brainstem and cerebellum in GM1 gangliosidosis mice. (wikigenes.org)

Here we demonstrate that the previously uncharacterized scaffolding protein Caskin2 is a novel regulator of EC quiescence and that loss of Caskin2 in mice results in elevated blood pressure at baseline. (duke.edu)
A study published in Nature, "Alpha-synuclein is a DNA binding protein that modulates DNA repair with implications for Lewy body disorders" (Schaser et al. (parkinson.org)
Moreover, in humans alphamacroglobulins such as pregnancy zone protein (PZP) and alpha-2-macroglobulin (A2M) also serve as immune-modulatory proteins where their normal functions within the body include binding and shuttling of protease inhibitors, growth factors, cytokines, hormones, disease factors, and various small molecule nucleophilic ligands. (hawaii.edu)
These mice express a catalytically inactive fusion protein of the NH 2 -terminal part of HDAC2 and β-galactosidase, which fails to integrate into corepressor complexes with mSin3B. (aacrjournals.org)
For example, this may mean the mouse starts to produce a new protein . (yourgenome.org)
Male mice without the protein consumed more alcohol than those that had it, while female mice without the protein consumed less than females with it. (concordia.ca)
The fact that mice lacking the protein have no obvious health problems is encouraging, suggesting that Abcc10 inhibitors could be used in human patients without causing side effects that might be expected to result from interfering with the pump's normal functions. (news-medical.net)
The mouse mismatch repair protein, MSH3, is a nucleoplasmic protein that aggregates into denser nuclear bodies under conditions of stress. (ru.nl)
The mouse MSH3-specific antibodies show that MSH3 is a nuclear protein with a finely-granular nucleoplasmic distribution, largely absent from areas of condensed heterochromatin. (ru.nl)
Myotubularin-related protein 14 (MTMR14) is a novel phosphoinositide phosphatase with roles in the maintenance of normal muscle performance, autophagy, and aging in mice. (biomedcentral.com)

Removing alpha-synuclein in mice (the knock-out mice) also resulted in increased DSBs, following bleomycin treatment. (parkinson.org)
Giving healthy human alpha-synuclein to the alpha-synuclein knock-out mice, restored the mice cells' DNA damage response to normal levels. (parkinson.org)
AOD 9604 was speculated to exhibit a potentially significant fat-burning impact when given to obese mice and beta(3)-AR knock-out mice for an extended period in a research published in 2001. (asianage.com)
In addition, the soma of the pyramidal cells in the frontal and parietal cortices are smaller in Fgf2 knock-out mice. (jneurosci.org)
Using HSL knock out mice, we were able to demonstrate that the lipase controls insulin secretion. (lu.se)

Likewise, loss of Caskin2 in vivo promotes increased vascular branching and permeability in mouse and zebrafish models. (duke.edu)

After four days of treatment with this inhibitor, normal mice showed an increase in brain triglycerides,' said Inloes. (news-medical.net)
Male KO mice exhibit increased fasting plasma total cholesterol and triglyceride concentrations relative to WT mice on the LF diet, but this difference disappears in HF diet-fed mice where there is increased cholesterol and triglycerides across all genotypes. (ox.ac.uk)
Results: Our results showed that HFD-induced mice with high expression of HKS in epididymal adipose tissue had slower weight gain, lower serum triglycerides, reduced free fatty acids, and improved liver insulin resistance compared with the Ad.Null group. (bvsalud.org)

However, they have consistently elevated systolic and diastolic blood pressure at baseline and significant context-dependent abnormalities in systemic metabolism (e.g., body weight, fat deposition, and glucose homeostasis). (duke.edu)
Our recent work revealed that MTMR14 KO mice weighed more than their WT littermates as adults, especially aged mice, which suggests that MTMR14 is involved in the regulation of body weight and metabolism. (biomedcentral.com)
They release the hormone insulin, which controls whole body metabolism. (lu.se)

The conditional deletion of NCB5OR in the mouse cerebellum and midbrain (conditional knock out, CKO mice) results in local iron dyshomeostasis and altered locomotor activity. (nih.gov)

Under normal diet, knockout had very little effect, but under high-fat diet, knockout mice showed a marked decrease in body weight and fat mass, as well as beneficial alterations in glucose and insulin sensitivity. (anl.gov)
Tpcn2 knockout mice have improved insulin sensitivity and are protected against high-fat diet-induced weight gain. (ox.ac.uk)
Our laboratory has shown that in response to a glucose challenge, two-pore channel 2 ( Tpcn2) knockout mice exhibit a decreased insulin response but normal glucose clearance, suggesting they have improved insulin sensitivity compared with wild-type mice. (ox.ac.uk)
We tested the hypothesis that improved insulin sensitivity in Tpcn2 knockout mice would protect against the negative effects of a high fat diet. (ox.ac.uk)
Despite the protection against diet-induced weight gain, however, Tpcn2 KO mice are not protected against HF-diet-induced changes in glucose or insulin area under the curve during glucose tolerance tests in female mice, while HF diet has no significant effect on glucose tolerance in the male mice, regardless of genotype. (ox.ac.uk)
Glucose disappearance during an insulin tolerance test is augmented in male KO mice, consistent with our previous findings suggesting enhanced insulin sensitivity in these mice. (ox.ac.uk)
These data demonstrate that knockout of Tpcn2 may increase insulin action in male, but not female, mice. (ox.ac.uk)
In addition, both male and female KO mice are protected against diet-induced weight gain, but this protection is likely independent from glucose tolerance, insulin sensitivity, and plasma lipid levels. (ox.ac.uk)
To examine the role of exosomes in AML12 insulin resistance, we used epididymal adipose tissue-derived exosomes or transfected Ad.HKS into mature 3T3L1-derived exosomes to interfere with palmitic acid (PA)-induced mouse AML12 insulin resistance model. (bvsalud.org)
This has implications both for normal control of insulin release as well as disease processes in islets, also known as lipotoxicity. (lu.se)
We showed how insulin deficiency in Friedreich's ataxia is likely to evolve, and that a mouse model for Huntington's disease develops diabetes due to loss of beta-cells. (lu.se)

Mice genetically engineered to lack this enzyme, known as NOX, show less brain injury after stroke. (vectorblog.org)
Clapham's team compared two groups of mice-normal "wild-type" mice and genetically engineered mice that were unable to make Hv1. (vectorblog.org)

Results: Targeted deletion of MLCK in adult mouse smooth muscle resulted in severe gut dysmotility characterized by weak peristalsis, dilation of the digestive tract, and reduction of feces excretion and food intake. (elsevierpure.com)

To evaluate the role of chondrocytes in this process, Mig6 was conditionally deleted from Col2a1-expressing cell types in the cartilage of mice.Methods Bone and cartilage in the synovial joints of cartilage-specific Mig6-deleted (knockout [KO]) mice and control littermates were compared. (bepress.com)
They are the first class 1 HDAC mutant mice that are viable although they are ∼25% smaller than their littermates. (aacrjournals.org)

The results were the same: a stark difference between the knockout and wild-type mice under high-fat conditions. (anl.gov)
Metabolic cage data revealed that 16 week old male CKO mice had elevated respiratory quotients and decreased respiratory water expulsion, decreased voluntary exercise, and altered feeding and drinking behavior compared to wild-type littermate controls. (nih.gov)
Serum leptin levels were significantly elevated in male CKO mice while prolactin, T3, and cortisol levels remain unchanged relative to wild-type controls, consistent with elevated transcript levels for leptin receptors (short form) in the male CKO mouse cerebellum. (nih.gov)
Previous reports from our laboratory have shown the up-regulation of alpha-macroglobulins in wild type (WT) mice after a lethal subcutaneous WNV infection. (hawaii.edu)
We further examined variations in the transcriptomes of testicular tissue between Znf230 KO and wild-type mice through microarray analysis. (scielo.org)
The results showed that the mRNA level of one unclassified transcript 4921513I08Rik was increased and that the mRNA levels of three other transcripts, i.e., 4930448A20Rik, 4931431B13Rik and potassium channel tetramerisation domain containing 14(Kctd14), were reduced more than two-fold in Znf230 KO mice compared with wild-type mice. (scielo.org)
Methods: Eight weeks-old wild type (WT) and Crabp1 knockout (CKO) mice were fed with a normal diet (ND) or high-fat diet (HFD) for 8 weeks. (tmu.edu.tw)
The general locomotor activity, motor balance, and coordination abilities were not statistically different between Slc20a2-HO and wild-type mice after adjusting for body weight, which was a major confounding factor in our motor function evaluations. (ox.ac.uk)

Consistently, the results indicate that FSH, independent of estrogen, increases the serum cholesterol level in this mouse model. (nature.com)
post-menopausal women with a higher serum FSH (≥78.3 IU/L) had higher serum TC and LDL-C levels than those with relatively lower FSH levels (40-78.3 IU/L), and ovariectomized mice had higher serum FSH and lipid levels and reduced hepatic LDL receptor expression. (nature.com)
We have previously reported that global ablation of NCB5OR in mice results in early-onset lean diabetes with decreased serum leptin levels and increased metabolic and feeding activities. (nih.gov)
Increased serum osmolality measurements confirmed the dehydration status of male CKO mice. (nih.gov)
We found that aged MTMR14 KO mice fed a normal chow diet exhibited increased serum triglyceride, total cholesterol, and glucose levels compared to age-matched WT controls. (biomedcentral.com)

Furthermore, we show for the first time that stress treatment of mouse cells with ethanol or hydrogen peroxide caused the re-distribution of MSH3 into nuclear bodies containing the proliferating cell nuclear antigen (PCNA), a known binding partner of MutSbeta. (ru.nl)

Children will be studied longitudinally and cross-sectionally to evaluate the factors linking child/parental microbiome with metabolic health, body composition, physical activity outcomes, psychological/behavioral, neurologic and physiological functions. (usda.gov)

Targeted knockout of Kmt2d in precursors cells of brown adipocytes and myocytes results in decreases in brown adipose tissue and muscle mass in mice, indicating that KMT2D is required for adipose and muscle tissue development. (wikipedia.org)
Recent studies have revealed numerous potential regulators of adult islet function, many of which are not expressed in mouse or immature human β-like cells. (einsteinmed.edu)
Importantly, these are unique to human beta cell/islet maturation: they are not expressed in adult mouse islet cells or β-like cells from renewable sources. (einsteinmed.edu)
These clumping proteins are called Lewy Bodies that can be found in an area of the brain stem where dopamine cells die. (parkinson.org)
However, DNA damage is also a normal result of cells undergoing constant wear-and-tear. (parkinson.org)
However, what if the unhealthy alpha-synuclein (which becomes Lewy Bodies) causes the loss of DSB repair, leading to healthy cells dying? (parkinson.org)
They tested this in two groups of live mice and live human cells: 1. (parkinson.org)
In both human cells in a dish (in vitro) and living mouse brains (in vivo) alpha-synuclein was present in the exact same location as the DNA repair proteins, suggesting alpha-synuclein binds directly to DSBs, and helps repair those breaks. (parkinson.org)
In both healthy human cells and living mouse brains, the laser-induced DSBs, triggered alpha-synuclein to move to the site of DNA damage. (parkinson.org)
In diseased human cells and mice carrying the abnormal form of alpha-synuclein, the laser-induced DSBs, impaired alpha-synuclein from moving to the site of DNA damage. (parkinson.org)
In the latest issue of the journal Nature, the scientists detail how mice that lack LPA receptors, which normally appear on the surface of cells in a mouse's womb, have fertility problems. (sciencedaily.com)
Huntingtin is expressed in the cytoplasm of most cells in the body. (jci.org)
This selectivity for cancer cells protects normal, healthy cells from side effects. (nanowerk.com)
While previous attempts have targeted FAK with liposomal nanoparticles or small molecule inhibitors, these methods are not tumor-specific and are more likely to harm normal cells, the scientists noted. (nanowerk.com)
The developmental stages of male germ cells of Znf230 KO mice were also normal. (scielo.org)
In the new research, Hopper-Borge and colleagues wanted to further explore, in both cultured cells and mice, the role of Abcc10. (news-medical.net)
Blastocysts containing cells, that are both wildtype and knockout cells, are injected into the uterus of a foster mother. (wikipedia.org)
For this example, we will take stem cells from a white mouse. (wikipedia.org)
Immune function refers to the ability of the immune system to recognize and destroy foreign invading foreign bodies (bacteria, fungi, viruses, etc.) and to remove senescent, damaged, dead and mutated cells as a mechanism of resistance to disease. (biomedcentral.com)
This problem was overcome by immunizing msh3-knockout mice and generating a panel of ten monoclonal antibodies, two of which localize MSH3 specifically in cultured mouse cells and bind to an epitope containing amino-acids 33-37. (ru.nl)
But since NOX is actually a whole family of enzymes, found in many kinds of cells in the body, inhibiting it would have too many side effects. (vectorblog.org)
The Hv1 "knockout" mice indeed had less ROS production in their brain cells. (vectorblog.org)

Calcified tissue in synovial joints was assessed by micro-computed tomography (micro-CT) and whole-skeleton staining.Results Formation of long bones was found to be normal in KO animals. (bepress.com)
Results of our study show that DKO mice are completely resilient to lethal flaviviral infections of WNV and JEV. (hawaii.edu)
As suggested by these results, a separate regulatory route for lipolysis may take precedence over the activity of ADRB3 when the animal's body weight sits closer to its normal range. (asianage.com)

The mouse and human KMT2D proteins are 5,588 and 5,537 amino acids in length, respectively. (wikipedia.org)

After 14 days of presentation, the compound AOD 9604 was suggested to lower both the weight and the amount of body fat in the obese mice. (asianage.com)
It was determined that the alleged capacity of AOD 9604 to raise the synthesis of beta-3 adrenergic receptors (also known as ADRB3 in obese mice) may have been responsible for this finding. (asianage.com)
IR and compensatory hyperinsulinaemia (HI) are present in 65-95% of women with PCOS, including the vast majority of overweight and obese women and more than half of women of normal weight. (biomedcentral.com)

Histone-lysine N-methyltransferase 2D (KMT2D), also known as MLL4 and sometimes MLL2 in humans and Mll4 in mice, is a major mammalian histone H3 lysine 4 (H3K4) mono-methyltransferase. (wikipedia.org)
The discovery that the LPA receptors affect fertility in mice may open a new area of fertility research and treatment for humans. (sciencedaily.com)
The mouse is closely related to humans with a striking similarity to us in terms of anatomy, physiology and genetics. (yourgenome.org)
Mice are currently the laboratory animal species most closely related to humans for which the knockout technique can easily be applied. (wikipedia.org)
The demonstration of what could happen was great, and they used mice because it's a more tractable system, but what we cared about were humans. (medscape.com)

Caskin2 knockout mice are born in normal Mendelian ratios and appear grossly normal during early adulthood. (duke.edu)

Scientists at The Scripps Research Institute are reporting that mice created to lack a certain type of molecule known as an LPA receptor have fertility problems, which suggests that these receptors play a major role in conception. (sciencedaily.com)

Millions of knockout mice are used in experiments each year. (wikipedia.org)

Next, the research team studied the effects of one particular taxane, paclitaxel, on mice and found that the knockout mice were more sensitive to the drug, as reflected in body weight, white blood cell count, and ability to survive escalating doses of the drug. (news-medical.net)
We analyzed the pathology of BRBV infection in mice and found a high sensitivity of the virus to the host interferon system. (cdc.gov)

The first recorded knockout mouse was created by Mario R. Capecchi , Martin Evans , and Oliver Smithies in 1989, for which they were awarded the 2007 Nobel Prize in Physiology or Medicine . (wikipedia.org)

Often, women will undergo treatments for infertility that range from taking hormones to stimulate ovulation to having their eggs harvested by doctors, fertilized by their partner's sperm outside their bodies, and finally having the early embryos implanted directly into their wombs (the technique of in vitro fertilization). (sciencedaily.com)

Circulating inflammatory cytokines were significantly elevated and plasma adipokine levels were abnormally regulated in aged MTMR14 KO mice. (biomedcentral.com)

Previous studies have shown that secondary metabolites of Bacillus subtilis strain Z15 (BS-Z15) are effective in treating fungal infections in mice. (biomedcentral.com)

Objective: This study aimed to investigate whether Slc20a2 knockout mice could recapitulate the dynamic processes and patterns of brain calcification and neurological symptoms in patients with PFBC. (ox.ac.uk)

In 1902, French biologist Lucien Cuénot was the first to demonstrate Mendel's theories of inheritance by highlighting the genetics of coat colour characteristics in mice. (yourgenome.org)

It has been established that lesion to or removal of the cerebellum leads to changes in nutrient organization, visceral response, feeding behavior, and body weight. (nih.gov)
This analysis showed no notable differences in pharmacokinetics with age (in postmenopausal women), race, or body weight (36 to 140 kg). (druglib.com)
this HF diet-induced increase in body weight is blunted in the Het and KO mice. (ox.ac.uk)
Body weight was lower in knockout mice as a consequence of reduced total body fat. (ox.ac.uk)
Normally, female rodents tend to consume more alcohol per body weight than males. (concordia.ca)
Body weight and food intake were monitored. (tmu.edu.tw)

Using our current examination techniques, these findings suggested that Znf230 deficiency in mice may not affect growth, fertility or spermatogenesis. (scielo.org)

However, the Rag2 knockout mouse showed significantly less species diversity. (broadinstitute.org)

The DKO mice had a significantly reduced inflammatory response which was characterized by lower concentrations of pro-inflammatory cytokines and chemokines in the blood, spleen, and brain of DKO mice when compared to WT. (hawaii.edu)
Here, we showed that anterior nucleus of paraventricular thalamus (PVA) plays an essential role in the development of mechanical hyperalgesia in neuropathic and inflammatory pain models in mice. (iasp-pain.org)

These mice provide evidence that the key functions of HDAC2, although not essential for survival of the organism, play a rate-limiting role for tumor development in vivo . (aacrjournals.org)
however, the tumor may develop anywhere in the body. (medscape.com)

To evaluate whether it also modulates immune function in mice to exert antifungal effects, we investigated the effect of BS-Z15 secondary metabolites on both the innate and adaptive immune functions of mice, and explored its molecular mechanism through blood transcriptome analysis. (biomedcentral.com)
BS-Z15 secondary metabolites were shown to enhance innate and adaptive immune function in mice, laying a theoretical foundation for its development and application in the field of immunity. (biomedcentral.com)
Background & Aims: Smooth muscle is essential for maintaining homeostasis for many body functions and provides adaptive responses to stresses imposed by pathologic disorders. (elsevierpure.com)

Echo MRI revealed normal body composition but decreased total water content and hydration ratios in CKO mice. (nih.gov)

However, in mice carrying defects in the type I and type II interferon system, the virus grew to high titers and caused severe pathology. (cdc.gov)

The biosynthesis and storage of catecholamines in chromaffin cell tumors may differ from the biosynthesis and storage in the normal medulla. (medscape.com)

The knockout males were seemingly unaffected by the loss of foxl3. (discovermagazine.com)

However, in laboratory mice, Thogotoviruses show an aggressive systemic infection affecting mainly the liver, lungs, and spleen, leading to a fatal acute hepatitis. (cdc.gov)

The transcription factor Nfix is essential for normal brain development. (ny.gov)
Brain MRIs from mice after stroke. (vectorblog.org)
That might include arthritis, cellular aging and even Alzheimer's disease-which also involves inflammation in the brain and which Clapham's team is in the early stages of investigating in mice. (vectorblog.org)

These mice showed symptoms similar to those of HSP patients, including abnormal gait and lower performance on tests of movement and cognition,' said Inloes. (news-medical.net)

Out of 40 antioxidant enzymes, only IDH1 expression was lost in multiple HuR-knockout cell lines, pointing to an HuR- IDH1 regulatory axis as a key component of the acute antioxidant response to stress 2 , 24 . (nature.com)
In contrast, no change in pyramidal or granule cell number is detected in the hippocampus of Fgf2 −/− mice. (jneurosci.org)
Specificity of the localization was demonstrated by absence of immunostaining in a cell line from the msh3-knockout mouse. (ru.nl)
We have acquired several advanced mass spectrometers over the years that permitted analyses of the metabolome in cell extracts and body fluids. (lu.se)

Anatomy3

Organisms8

Diseases1

Analytical, Diagnostic and Therapeutic Techniques and Equipment2

Phenomena and Processes1

C57BL2

Gene31

Homozygous3

Embryonic6

Obesity4

Heterozygous2

Neurons6

Phenotype3

Genes7

Researchers4

Tissues3

Deficient5

Strains3

Genetic3

Postnatal2

Protein10

Knock-out5

Zebrafish1

Triglycerides3

Metabolism3

Conditional1

Insulin11

Genetically2

Targeted deletion1

Littermates2

Wild-type8

Serum5

Proliferating cell nu1

Factors1

Cells22

Results3

Proteins1

Obese3

Humans5

Mendelian1

Scientists1

Experiments1

Found2

Physiology1

Vitro1

Significantly elevated1

Strain1

Investigate1

Demonstrate1

Weight6

Findings1

Species1

Inflammatory2

Tumor2

Adaptive3

Ratios1

Pathology1

Biosynthesis1

Males1

Systemic1

Brain3

Including abnormal1

Cell4