• ELISA
  • First of all, it should be noted that the Western blot is usually performed as a follow-up to an ELISA test, which is the most commonly employed initial test for Lyme disease. (google.com)
  • for example, the usual testing regime for HIV is also an initial ELISA followed by a confirmatory Western blot. (google.com)
  • Both the ELISA and the Western blot are "indirect" tests -- that is, they measure the immune system's response to an infectious agent rather than looking for components of the agent itself. (google.com)
  • assay
  • The following article is offered as a primer in the techniques and interpretation of Western blotting, and should help most patients navigate their way through some of the medical and scientific terminology associated with the assay. (google.com)
  • method
  • The traditional Western blot can be a labor-intensive and time-consuming process, leading many researchers to seek an alternative method that is more efficient, reproducible and quantitative. (novusbio.com)
  • nylon
  • This is done by first disrupting Bb cells with an electrical current and then "blotting" the separated proteins onto a paper or nylon sheet. (google.com)
  • step
  • While the step-by-step process of a western blot has for the most part remained the same over the years, variations in solutions, procedures and reagents may increase the efficacy of your results. (novusbio.com)
  • control
  • The signal intensity obtained can then be normalized with respect to the signal intensity of the loading internal control detected on the same gel or blot. (wikipedia.org)
  • cells
  • I first ran a Western blot with 15ug of a RIPA whole cell lystate from WKPT cells a rat kidney immortalized cell line derived from the S1 proximal tubule segment. (novusbio.com)
  • tests
  • Started dating someone with both HSV1 & 2 and had some oral symptoms, so my doctor ran blood tests again - this time using Western Blot through Quest Labs. (medhelp.org)