• cDNA
  • Commonly cDNA is created with labelled primers for the RNA sequence of interest to act as the probe in the northern blot. (wikipedia.org)
  • The reverse northern blot is a method by which gene expression patterns may be analyzed by comparing isolated RNA molecules from a tester sample to samples in a control cDNA library. (wikipedia.org)
  • Size markers are indicated at the left side of C. The blot was hybridized with the entire insert from the full-length cDNA clone for RMCP-8 and the blot was washed under medium stringency conditions (2 × SSC and 0.5% SDS at 65°C). (nih.gov)
  • membranes
  • Blots are made of high quality total RNA samples which are subjected to rigorous quality control procedures before fractionation through large denatured agarose gels and blotting onto positively charged nylon membranes. (zyagen.com)
  • gels
  • The gels can be stained with ethidium bromide (EtBr) and viewed under UV light to observe the quality and quantity of RNA before blotting. (wikipedia.org)
  • Reverse
  • Reverse northern blotting was used in a 2013 study in Gene in which the author identified a number of genes responsible for early cold-resistance response in the cold-hardy citrus fruit Poncirus trifoliata. (wikipedia.org)
  • tissue
  • If you are interested in Northern blot constructed with any mouse RNA tissue of any strain or required blots to be specifically prepared to meet your application, our custom service scientists can make it for you. (zyagen.com)
  • samples
  • To create controls for comparison in a northern blot, samples not displaying the gene product of interest can be used after determination by microarrays or RT-PCR. (wikipedia.org)
  • total
  • Blots are made of total RNA that is treated with RNase-free DNase to remove residual DNA. (zyagen.com)
  • room
  • Blots are shipped in a sealed bag at room temperature and are good for 1 year if stored at 4 o C in the sealed bags. (zyagen.com)