• 1977
  • The northern blot technique was developed in 1977 by James Alwine, David Kemp, and George Stark at Stanford University, with contributions from Gerhard Heinrich. (wikipedia.org)
  • In order to avoid confusion, the following abbreviations will be used consistently throughout this article: Since its introduction in 1977, northern blot had been used extensively for RNA quantification despite its shortcomings: (a) time-consuming technique, (b) requires a large quantity of RNA for detection, and (c) quantitatively inaccurate in the low abundance of RNA content. (wikipedia.org)
  • membranes
  • Blots are made of high quality total RNA samples which are subjected to rigorous quality control procedures before fractionation through large denatured agarose gels and blotting onto positively charged nylon membranes. (zyagen.com)
  • tissue
  • If you are interested in Northern blot constructed with any mouse RNA tissue of any strain or required blots to be specifically prepared to meet your application, our custom service scientists can make it for you. (zyagen.com)
  • protein
  • There also exists a similar, but different method of detecting protein s called a Western Blot . (everything2.com)
  • The other blotting techniques emerged from this method have been termed as Northern (for RNA), Western (for proteins), Eastern (for post-translational protein modifications) and Southwestern (for DNA-protein interactions) blotting. (sigmaaldrich.com)
  • researchers
  • To determine what makes grey horses more susceptible to melanomas, researchers have used different techniques such as the Northern Blot technique and Real-Time PCR. (wikipedia.org)
  • gels
  • The gels can be stained with ethidium bromide (EtBr) and viewed under UV light to observe the quality and quantity of RNA before blotting. (wikipedia.org)
  • Reverse
  • Reverse northern blotting was used in a 2013 study in Gene in which the author identified a number of genes responsible for early cold-resistance response in the cold-hardy citrus fruit Poncirus trifoliata. (wikipedia.org)
  • total
  • Blots are made of total RNA that is treated with RNase-free DNase to remove residual DNA. (zyagen.com)
  • samples
  • To create controls for comparison in a northern blot, samples not displaying the gene product of interest can be used after determination by microarrays or RT-PCR. (wikipedia.org)
  • room
  • Blots are shipped in a sealed bag at room temperature and are good for 1 year if stored at 4 o C in the sealed bags. (zyagen.com)