Blood tumor cells cultured. Medical search. Frequent questions

In line with this observation, elevated extracellular lactate concentrations up to 40 mM and corresponding low extracellular pH (pH e ) have been found in solid tumors under hypoxic conditions [ 18 - 22 ]. (hindawi.com)
Given these findings, AFM24 is now being evaluated in a phase 1/2 trial (NCT04259450) in patients with advanced EGFR -positive solid tumors. (onclive.com)
Conclusion This study represents a crucial step in the mechanistic understanding and safety of Cish targeting to unleash NK cell antitumor function in solid tumors. (bmj.com)
Its diverse TCR clonality, superior tumor-homing ability, and low off-target toxicity endow TIL therapy unique advantages in treating solid tumors compared with other adoptive cellular therapies. (biomedcentral.com)
Solid tumors possess multiple adverse factors in an immunosuppressive microenvironment that impair CAR-T cell infiltration and function. (bvsalud.org)

PBMCs from cancer patients can be extracted and cultured in vitro. (wikipedia.org)
Distinct hallmarks of cancer were examined using in silico analysis, various in vitro cell culture assays, and ex vivo organotypic rat brain slice culture model. (hindawi.com)
We investigated the impact on tumor malignancy in F98 glioma cells using in silico analysis, in vitro cell culture assays, and ex vivo vascular organotypic glioma impact model (VOGIM) [ 35 ] by implanting F98 cells into rat brain slices. (hindawi.com)
Adult stem cell-based organoid technology is a versatile tool for the generation and long-term maintenance of near-native 3D epithelial tissues in vitro. (nature.com)
In vitro generation of human pluripotent stem cell derived lung organoids. (nature.com)
In vitro , the sprouting and migratory properties of primary endothelial cell cultures were reduced by inhibiting EZH2 through up-regulation of miR-101, siRNA-mediated knockdown of EZH2, or treatment with 3-Deazaneplanocin-A (DZNep), a small molecule inhibitor of EZH2 methyltransferase activity. (plos.org)
Finally, EPB41L4A-AS2 inhibited breast cancer cell proliferation, migration and invasion and induced cell apoptosis in vitro. (cancerindex.org)
There is an unmet clinical need to extract living circulating tumor cells (CTCs) for functional studies and in vitro expansion to enable drug testing and predict responses to therapy in metastatic cancer. (lu.se)
The novel A2 method provides extensive elimination of WBCs combined with the gentle recovery of viable cancer cells suitable for downstream functional analyses and in vitro culture. (lu.se)
Because cells in vivo interact with their environment in three dimensions, 3D cell culture tools, reagents, and techniques have led to the creation of more predictive in vitro cell models for diverse applications and disciplines including cancer research, drug discovery, neuroscience, and regenerative medicine. (sigmaaldrich.com)
The changes of immune cells and cytokines in vitro and in vivo were detected by flow cytometry and enzyme-linked immunosorbent assay (ELISA). (biomedcentral.com)
In vivo and vitro, Niraparib can also increase the proportion of T cells and combined with PD-L1 blockade could further enhance the effect. (biomedcentral.com)
MST-1 cells grown in vitro were heterogeneous in morphology, ranging from floating round cells, loosely attached round/oval or elongated cells with prominent pseudopod-like processes, to well-attached spindle and elongated dendritic cells without obvious pseudopods. (tmu.edu.tw)
NK cells cytokine expression, signaling and cytotoxicity has been evaluated in vitro. (bmj.com)
Generating perfusable 3D microvessels in vitro is an important goal for tissue engineering, as well as for reliable modelling of blood vessel function. (rsc.org)
To date, in vitro blood vessel models have not been able to accurately reproduce the dynamics and responses of endothelial cells to grow perfusable and functional 3D vascular networks. (rsc.org)
Tumor-infiltrating lymphocyte (TIL) therapy is a type of adoptive cellular therapy by harvesting infiltrated lymphocytes from tumors, culturing and amplifying them in vitro and then infusing back to treat patients. (biomedcentral.com)
To this end, Dr. Rosenberg and others developed a "Young TIL" approach that rapidly expands TIL for administration without in vitro selection for tumor reactivity [ 8 ], which markedly improves the timeliness of TIL production as well as its survival and efficacy in vivo (Fig. 1 ). (biomedcentral.com)
Currently, the most widely-used TIL production method is to isolate infiltrating lymphocytes from tumor tissues and then culture and expand these cells in vitro. (biomedcentral.com)
We simultaneously knocked down immune checkpoints and immunosuppressive molecular receptors in CAR-T cells by constructing two clusters of small hairpin RNAs and evaluated the engineered CAR-T cells for antitumor activity both in vitro, using tumor cell lines and cholangiocarcinoma organoid models, and in vivo, using humanized mouse models. (bvsalud.org)
Those cells, named PTG-T16R-scFV-CAR-T cells, potently killed tumor cells in vitro and promoted apoptosis of tumor cells in a cholangiocarcinoma organoid model. (bvsalud.org)
CONCLUSIONS: Our results revealed that PTG-T16R-scFV-CAR-T cells with knockdown of sextuplet inhibitory molecules exhibited strong immunity against cholangiocarcinoma and long-term efficacy both in vitro and in vivo. (bvsalud.org)
Described here are some of the general requirements of in vitro test methods for skin sensitization, and progress that has been made in developing suitable approaches with particular emphasis on the utility of dendritic cell culture systems. (cdc.gov)

Subsequently, PBMCs are challenged with tumor antigens such as tumor stem cell antigen. (wikipedia.org)
Cell surface antigens are large molecules sticking out from a cell that help the immune system determine whether the cell is harmless or harmful. (stanford.edu)
ICE is a bispecific engager of CD16A+ natural killer (NK) cells and macrophages and tumor antigens. (onclive.com)
After these initial studies had yielded such promising results, Lukas Bunse expects that vaccine-induced T-cell receptors targeting brain tumor antigens could be a promising approach to develop new immunotherapies against glioblastoma. (dkfz.de)
According to the scientist, it is also conceivable to generate T cell receptor transgenic cell therapies against patient-individual cancer antigens. (dkfz.de)
Immunostaining revealed that major melanoma-associated antigens, such as S100 protein, HMB-45, melanotransferrin, chondroitin sulphate proteoglycan, and the gangliosides GD2 and GD3, were consistently expressed by the tumour tissue, severe combined immunodeficiency (SCID) mouse xenograft and derived cell lines. (tmu.edu.tw)
This observation and the expression of the major melanoma-associated antigens were all indicative of the melanocytic origin of MST-1 tumour. (tmu.edu.tw)
The availability of the soft tissue melanoma cell line, the SCID mouse xenograft tumour system as well as autologous TILs described herein would provide useful materials for identifying T-cell-defined antigens as well as a model system for devising individualised cancer biotherapeutic strategies. (tmu.edu.tw)
Subsequently, we engineered CAR-T cells targeting EGFR and B7H3 antigens. (bvsalud.org)
Tumor Antigens Many tumor cells produce antigens, which may be released in the bloodstream or remain on the cell surface. (msdmanuals.com)
Concomitant use of interferon enhances the expression of major histocompatibility complex (MHC) antigens and TAAs on tumor cells, thereby augmenting the killing of tumor cells by the infused effector cells. (msdmanuals.com)
Tests such as ELISpot enumerate cells releasing IFN-g after mononuclear cells recovered from whole blood are incubated with similar antigens. (cdc.gov)

TAK-285 was examined in a model of breast cancer brain metastasis using direct intracranial injection of BT-474-derived luciferase-expressing cells and showed greater inhibition of brain tumor growth compared to animals treated with lapatinib. (jcancer.org)
This brain tumor type is highly proliferative and shows an infiltrative growth pattern, accounting for the high recurrence rates in patients. (hindawi.com)
Swedish Research Counsil (R.J.A.N.), National Cancer Institute (NCI) CA069246 and CA86355 (X.O.B.), and the American Brain Tumor Association (T.W.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. (plos.org)
Engineered human immune cells can vanquish a deadly pediatric brain tumor in a mouse model, a study from the Stanford University School of Medicine has demonstrated. (stanford.edu)
Lukas Bunse, a scientist at the German Cancer Research Center (Deutsches Krebsforschungszentrum, DKFZ) and a physician at the University Medical Center Mannheim, relies on the comparatively new concept of "T cell receptor transgenic cells" in his current study: To this end, brain tumor patients were first inoculated with an antigenic fragment of the protein NLGN4X (Neuroligin4X). (dkfz.de)
Bunse's team then demonstrated that the NLGN4X-specific T cells are able to kill brain tumor cells in the culture dish. (dkfz.de)
Brain tumor-bearing mice treated with transgenic NLGN4X-specific human T cells had a greater than 40 percent response to treatment. (dkfz.de)

We use primary organoid cultures of diverse tissues and tumor biopsies for immunotherapy modeling, oncogene functional screening and stem cell biology. (stanford.edu)
Scientists from the German Cancer Research Center (DKFZ) and the University Medical Center Mannheim (UMM) successfully tested a new form of cellular immunotherapy against brain tumors in mice for the first time. (dkfz.de)
Increasing evidence indicated that conventional and targeted therapies could affect tumor-associated immune responses and increase the effectiveness of immunotherapy. (biomedcentral.com)
In passive cellular immunotherapy, specific effector cells are directly infused and are not induced within the patient. (msdmanuals.com)
Passive cellular immunotherapy references A number of immunologic interventions, both passive and active, can be directed against tumor cells. (msdmanuals.com)
In passive cellular immunotherapy, specific effector cells. (msdmanuals.com)
CAR T cell immunotherapy for human cancer. (msdmanuals.com)

In the metabolic symbiosis model, these cells have been proposed to overexpress MCT4 to release high amounts of lactate produced during anaerobic glycolysis into the tumor microenvironment. (hindawi.com)
Over the last decade, significant investigation in the MM field has demonstrated that the interaction of malignant PCs with BM stromal cells within the microenvironment is essential for survival of the PC clone [ 5 , 6 ]. (oncotarget.com)
The observation of this intercellular communication and that MM cells modify the microenvironment is highly suggestive that one of the first biologically relevant changes necessary for progression of MGUS or SMM to MM is for a member(s) of the clone to evolve and acquire the molecular changes needed to reprogram its immediate microenvironment into a supportive, growth-promoting microenvironment. (oncotarget.com)
3D cell culture methods - and multicellular tumor spheroids (MCTS), in particular - are quickly becoming a valuable tool for studying the tumor microenvironment in cancer research . (corning.com)
Cancer scientists are creating increasingly complex tumor models that more accurately replicate in vivo conditions - including the tumor microenvironment, which plays a key role in tumor progression, according to a study published in BioTechniques . (corning.com)
modeling it in the tumor microenvironment, though, has been challenging. (corning.com)
Patient-derived organoid (PDO) has great potential in precision oncology, but low success rate, time-consuming culture, and lack of tumor microenvironment (TME) limit its application. (bvsalud.org)
We found an abundance of programmed cell death protein 1 (PD-1), T cell immunoglobulin and mucin domain-containing protein 3 (Tim-3), and T cell immunoglobulin and ITIM domain (Tigit) on infiltrated CD8+ T cells in the cholangiocarcinoma microenvironment. (bvsalud.org)

Breast cancer is one of the most common primary tumors to develop associated brain metastases ( 1 , 2 ), and while recent progress in cancer therapy has improved control of primary breast tumors, methods to effectively treat brain metastases from breast cancer remain inadequate. (jcancer.org)
IPA analysis of the 280 significantly differentially expressed genes in the whole blood was suggestive of increased hematopoiesis and cell development, activation of cancer/tumor development pathways, and atopy. (cdc.gov)
Besides, upregulated MCT4 has been linked to altered tumor metabolism as well as to increased growth and survival in breast and pancreatic cancer [ 33 , 34 ]. (hindawi.com)
For this purpose, we designed an MCT4 overexpression and knockdown/inhibition model to explore the functional consequences of MCT4 expression on cancer cell metabolism, survival, proliferation, migration, invasion potential, and angiogenesis as well as on cell cycle profiles and cell death mechanisms. (hindawi.com)
The generation of cancer organoids from primary patient material enables a range of therapeutic agents to be tested in the resulting organoid cultures. (nature.com)
Tumor evolution and drug response in patient-derived organoid models of bladder cancer. (nature.com)
[ 115-117 ] In cancer cell line spike-in experiments, this method was capable of detecting one cancer cell per 5 ml blood. (medscape.com)
Also, cathepsin D or MUC1 secreting cells were successfully detected in breast cancer patients but not in healthy controls. (medscape.com)
[ 115 ] Moreover, CK19 releasing cells were found to be detectable in up to 65 and 70% of CRC and breast cancer patients, respectively, and correlated with presence of metastasis and poor survival in breast cancer. (medscape.com)
Although cancer cell lines and animal models have revealed much information about intestinal cancers, cancer-derived intestinal organoids have been demonstrated to more faithfully recapitulate the tissue architecture, cellular heterogeneity, and morphology of the originating tumor. (stemcell.com)
1 Cancer-derived organoids are consequently proving to be useful experimental models for investigating cancer biology, including disease progression and the affected signaling pathways and niche requirements of the tumor. (stemcell.com)
2 Cancer-derived organoids have also enabled more translational applications, such as activating and expanding tumor-reactive T cell populations, predicting patient-specific treatment outcomes, and screening potential therapeutics. (stemcell.com)
Angiogenesis - the formation of new blood vessels - occurs during tissue growth and development, but also during wound healing and cancer [1] , [2] . (plos.org)
VEGF, produced in large amounts by cancer cells during tumor growth, interacts with its receptors VEGFR1 and VEGFR2 thereby causing endothelial cell survival, proliferation, and sprouting [7] . (plos.org)
A new study explains how a single bout of high-intensity exercise can boost tumor-suppressing mechanisms even in advanced prostate cancer. (medicalnewstoday.com)
Previous research has shown that blood taken from patients with advanced prostate cancer who followed a high-intensity exercise program for a few months could suppress the growth of cultured tumor cells. (medicalnewstoday.com)
These results suggest that regular exercise could help suppress tumor cell proliferation in patients with advanced prostate cancer, and each additional bout of exercise could potentially further enhance this tumor-suppressive effect. (medicalnewstoday.com)
Previous research has shown that serum from advanced prostate cancer patients who followed a supervised high-intensity exercise program (HIIT) for 6 months showed elevated myokine levels and a greater ability to suppress the growth of tumor cells. (medicalnewstoday.com)
In a follow-up study published in Prostate Cancer and Prostatic Diseases , the same researchers have now shown that serum samples collected immediately after a single bout of physical exercise from exercise-trained advanced prostate cancer patients also showed elevated myokine levels and enhanced tumor suppressive effects than before exercise. (medicalnewstoday.com)
However, mutations in prostate cancer cells can lead to the development of resistance to androgen deprivation therapy. (medicalnewstoday.com)
Physical exercise can modulate the circulating levels of growth factors, hormones, and cytokines, a class of proteins that can modulate inflammation levels, that are involved in sustaining the growth of cancer cells. (medicalnewstoday.com)
Consistent with this, studies have shown that serum collected immediately after a bout of exercise from patients with breast or colon cancer can inhibit the proliferation of cultured cancer cells. (medicalnewstoday.com)
These molecules that can suppress cancer cell growth include myokines, which are cytokines and other proteins secreted by the skeletal muscle fibers during physical activity. (medicalnewstoday.com)
In mice, a fatal brainstem tumor was cleared by injecting it with engineered T cells that recognized the cancer and targeted it for destruction. (stanford.edu)
In mice whose brainstems were implanted with human DIPG, engineered immune cells known as chimeric antigen receptor T cells - or CAR-T cells - were able to eliminate tumors, leaving very few residual cancer cells. (stanford.edu)
When the brains of the mice were examined via immunostaining after treatment, the animals had, on average, a few dozen cancer cells left, compared with tens of thousands of cancer cells in animals that received a control treatment. (stanford.edu)
As a cancer immunotherapist, what gets me really excited is when you take an established tumor and you make it disappear," said Crystal Mackall , MD, professor of pediatrics and of medicine and the study's other senior author. (stanford.edu)
In CAR-T therapies now used in humans, some of the patient's own immune cells are removed, engineered to attack a surface antigen on the cancer cells, and returned to the patient's body, where they target the cancer cells for destruction. (stanford.edu)
Scientists have known for decades that GD2 levels on some other forms of cancer are very high, but its discovery on this tumor came as a surprise, Mackall said, adding, "It was hiding in plain sight, and we didn't know. (stanford.edu)
Other CAR-T cells that were tuned to different molecular targets also did not kill the DIPG cancer cells. (stanford.edu)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Acute lymphoblastic leukemia (ALL) is a cancer of the white blood cells and is typically well treated with combination chemotherapy, with a remission state after 5 years of 94% in children and 30-40% in adults. (karger.com)
B7H6-specific BiTE therapy enhanced the survival of lymphoma bearing mice and decreased tumor burden of melanoma and ovarian cancer bearing mice. (bioskinrevive.com)
Cancer examples had been mechanically disrupted and reddish colored blood cells had been lysed with Doripenem Hydrate ACK lysis buffer (0.15M NH4Cl 10 KHCO3 0.1 EDTA pH 7.4). (bioskinrevive.com)
In phytochemistry, plant saponins are well known to have a wide spectrum of activities including lowering blood cholesterol, inhibiting cancer cell growth, and acting as antifungal and antibacterial agents. (shamansgarden.com)
A group from Nagoya University has identified the optimal dimensions of glass filters for circulating tumor cells (CTCs) and cancer-associated fibroblasts (CAFs), demonstrating that they could detect cells from lung cancer patients. (nagoya-u.ac.jp)
Their findings will enable more efficient culture of tumor cells to identify patients with cancer. (nagoya-u.ac.jp)
Analyzing these cells provides direct information about cancer and can predict disease progression even in the early stages of the disease. (nagoya-u.ac.jp)
However, the number of CTCs in the blood of cancer patients is extremely low. (nagoya-u.ac.jp)
Landscape of tumor suppressor long noncoding RNAs in breast cancer. (cancerindex.org)
The landscape and biological functions of tumor suppressor long noncoding RNAs in breast cancer are still unknown. (cancerindex.org)
Data from whole transcriptome sequencing of 33 breast specimens in the Harbin Medical University Cancer Center cohort and The Cancer Genome Atlas was applied to identify and validate the landscape of tumor suppressor long noncoding RNAs, which was further validated by The Cancer Genome Atlas pancancer data including 33 cancer types and 12,839 patients. (cancerindex.org)
Next, the expression model, prognostic roles, potential biological functions and epigenetic regulation of tumor suppressor long noncoding RNAs were investigated and validated in the breast cancer and pancancer cohorts. (cancerindex.org)
Finally, EPB41L4A-AS2 was selected to validate our novel finding, and the tumor suppressive roles of EPB41L4A-AS2 in breast cancer were examined. (cancerindex.org)
In this study, we aimed to evaluate the possible effects of tumor-stromal interactions on local estrogen biosynthesis in endometrial cancer. (scirp.org)
We isolated stromal cells from endometrial cancer patients to examine local biosynthesis of estrogens and tumor-stromal interactions. (scirp.org)
Next we examined the effects of steroid sulfatase inhibitor and aromatase inhibitor in monoculture of endometrial cancer cell line (Ishikawa) and in a co-culture system involving an Ishikawa cells and stromal cells. (scirp.org)
Estrogen receptor and steroid sulfatase mRNA levels in cancer cells were significantly higher in the co-cultures compared with the monocultures of endometrial cancer cells. (scirp.org)
Proliferation of the cancer cells was significantly increased through the steroid sulfatase pathway, which metabolizes androgens, estrone sulfate, and estradiol sulfate as its substrates. (scirp.org)
We evaluated the effects of STS inhibitor and aromatase inhibitors on the proliferation of estrogen-dependent endometrial cancer cells. (scirp.org)
Here, we present a novel two-step acoustophoresis (A2) method for isolation of unfixed, viable cancer cells from red blood cell (RBC) lysed whole blood. (lu.se)
This acoustofluidic step enriches viable cancer cells in a central outlet, but a significant number of white blood cells (WBCs) remain in the central outlet fraction due to overlapping acoustophysical properties of these viable cells. (lu.se)
Using samples with 10 000 DU145 cells, we obtained 459 ± 188-fold depletion of WBC and 42% recovery of viable cancer cells. (lu.se)
Current Research and Scholarly Interests We study cancer biology, intestinal stem cells (ISC), and angiogenesis. (stanford.edu)
Angiogenesis projects include blood-brain barrier regulation, stroke therapeutics and anti-angiogenic cancer therapy. (stanford.edu)
Common examples include cancer tumorspheres, which enable the study of oxygen gradients and nutrient access in tumor formation. (sigmaaldrich.com)
In this way, they succeeded in producing large numbers of T cells with identical specificity, all of which recognize the cancer antigen NLGN4X. (dkfz.de)
In melanoma, the malignant black skin cancer, such T-cell receptor transgenic T cells have already been shown to prolong the lives of some patients. (dkfz.de)
Programmed death ligand 1 (PD-L1) expression in human ovarian cancer cells after PARP inhibitors treatment was examined by western blotting (WB) and flow cytometry. (biomedcentral.com)
Niraparib upregulated membrane PD-L1 and total PD-L1 expression in ovarian cancer cells and had a synergistic effect with PD-L1 blockade in vivo. (biomedcentral.com)
Despite the profound and sustained anti-tumor responses observed in treating ovarian cancer patients, resistance to PARP inhibitors has emerged in some cases [ 8 ]. (biomedcentral.com)
They also showed how amyloid beta achieves this feat: by tamping down the body's normal immune response against cancer cells that make it into the brain. (cancer.gov)
In fending off the immune response, the protein buys the cancer cells time to grow into full-fledged tumors. (cancer.gov)
Her team has been studying the mechanisms that cancer cells use to spread to and thrive in the brain. (cancer.gov)
The team next performed a series of experiments using cancer cells that could and couldn't produce amyloid beta. (cancer.gov)
To reach and colonize the brain, cancer cells need to overcome several hostile environments, explained Dr. Gril. (cancer.gov)
This cell line can also be used for further studies aimed at uncovering the histogenesis of this rare cancer. (tmu.edu.tw)
Using intravenous injection of B16F10 melanoma cell line and EO711 triple negative breast cancer cell line, metastasis evaluation was performed. (bmj.com)
Cish fl/fl Ncr1 Ki/+ NK cells display lower activation thresholds and Cish fl/fl Ncr1 Ki/+ mice are more resistant to tumor metastasis and to primary breast cancer growth. (bmj.com)
Circulating Tumour Cells (CTCs) and circulating tumour DNA (ctDNA) represent a non-invasive liquid biopsy approach for the follow-up and therapy management of cancer patients. (oncotarget.com)
Here we describe a microfluidic-based platform whereby we model natural cellular programs found during normal development and angiogenesis to form perfusable networks of intact 3D microvessels as well as tumor vasculatures based on the spatially controlled co-culture of endothelial cells with stromal fibroblasts, pericytes or cancer cells. (rsc.org)
Combination of IL-2/15/21 can enhance TIL expansion in lung and colorectal cancer and promote CD8+ T cell percentage as well as TCR clone diversity compared to IL-2 alone [ 13 ]. (biomedcentral.com)
But when it comes to cancer, interest in an alkaline diet mostly stems from laboratory studies suggesting that cancerous tumor cells have an acidic environment surrounding them. (aicr.org)
Studies of isolated cell cultures and animal studies of cancer don't necessarily represent what happens within the human body. (aicr.org)
The ECM a researcher chooses has a significant effect on cancer cell proliferation, initiation, invasion, and metastasis, and it alters how the tumor model responds to drug therapy. (corning.com)
Collagen type I hydrogels have been used to grow MCTS from osteosarcomas, breast cancer cells, human colorectal cancer cells, prostate cancer cell lines, and primary cancer cells from colorectal cancer patients, according to the Science Advances study. (corning.com)
Personalized cancer therapy is emerging as a critical strategy for treating tumors. (corning.com)
MCTS can mimic metastatic microtumors, which form when cancer cells spread from the site of the primary tumor via intravasation into the blood circulation and via extravasation into the parenchyma of an organ. (corning.com)
Spheroids have also been used to study transcoelomic metastasis in ovarian cancer, in which primary tumor cells seep into the abdominal cavity, where they form spheroids and then travel through fluids to secondary sites. (corning.com)
HCC-PDOs and PBMC are co-cultured with MSC and Cancer-associated fibroblasts (CAF). (bvsalud.org)
Animal studies have shown that LAK cells are more effective against cancer cells than are the original endogenous T cells, presumably because of their greater number. (msdmanuals.com)
Therefore CAR T cells and TCR T cells may represent complementary approaches to cancer therapy. (msdmanuals.com)
diagnostic prediction of small round blue cell tumors (SRBCT) of childhood and determining the estrogen receptor (ER) status of sporadic breast cancer. (lu.se)
Transcriptomic profiling of T-cell populations in non-muscle invasive and muscle invasive bladder cancer. (lu.se)

Whole blood gene expression profiling is a promising approach to identification of novel biomarkers for tissues over traditional, invasive biopsy. (cdc.gov)
When cells are not grown on supports, they may form 3D aggregates called spheroids, which secrete their own ECM to become more like native solid tissues. (sigmaaldrich.com)
Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
Gottlieb Haberlandt first pointed out the possibilities of the culture of isolated tissues, plant tissue culture . (wikipedia.org)
[12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
C. Peterson, M. Ringne´r / Artificial Intelligence in Medicine 28 (2003) 59-74 structure and function, cellular metabolism, development of cells and tissues, and response of organisms to their environments. (lu.se)
Since genes are composed of a given alphabet (A, T, C, G) with fixed pairing properties A-T and C-G, probes can be constructed that attract gene transcripts extracted from cell tissues and cultures. (lu.se)

A peripheral blood mononuclear cell (PBMC) is any peripheral blood cell having a round nucleus. (wikipedia.org)
Largely the product of mononuclear phagocyte activation, it is unclear whether TNF-α production at disease sites is a feature of all mononuclear phagocytes that accumulate there or whether it is secreted by a subset of these cells. (ersjournals.com)
This study has demonstrated that a subset of mononuclear phagocytes, mature macrophages, are responsible for tumour necrosis factor secretion and this could have implications for targeted management in sarcoidosis in the future. (ersjournals.com)
Pathologically, at an early stage, there is an influx of mononuclear cells followed by lymphocytes. (ersjournals.com)
However, it is not clear whether TNF production in disease sites is a feature of all mononuclear cells that traffic there or whether it is a subset phenomenon. (ersjournals.com)
To do so, monocyte-differentiated macrophages from healthy donor peripheral blood mononuclear cells were co-cultured for 4 hours with CMFDA-labeled, wild-type EGFR-expressing or KRAS -mutant tumor cells in the presence of 10 µg/mL of AFM24 or cetuximab. (onclive.com)
Activated peripheral blood mononuclear cells (PBMCs) were added to spheroids and their activity monitored over time using high-content imaging. (selectscience.net)
Here, MSC and peripheral blood mononuclear cells (PBMC) coculture is used to construct HCC organoid-on-a-chip mimicking original TME and provide a high-throughput drug-screening platform to predict outcomes of anti-HCC immunotherapies. (bvsalud.org)

To investigate the chromosomal fragility, we researched by cytogenetic technique in the peripheral blood lymphocytes cultures and in the tumors cells cultures from canines presenting oral and cutaneous warts lesions. (fiocruz.br)
These cells consist of lymphocytes (T cells, B cells, NK cells) and monocytes, whereas erythrocytes and platelets have no nuclei, and granulocytes (neutrophils, basophils, and eosinophils) have multi-lobed nuclei. (wikipedia.org)
In humans, lymphocytes make up the majority of the PBMC population, followed by monocytes, and only a small percentage of dendritic cells. (wikipedia.org)
Tumor-infiltrating lymphocytes (TILs) may have greater tumoricidal activity than LAK cells. (msdmanuals.com)
Cutaneous T-cell lymphoma (CTCL) (see the image below) is a heterogeneous group of lymphoproliferative disorders characterized by localization of neoplastic T lymphocytes to the skin, with no evidence of extracutaneous disease at the time of diagnosis. (medscape.com)

Protein markers commonly used for circulating tumor cells investigations. (medscape.com)

The presence of CTCs in 43 blood samples from patients with metastatic melanoma was evaluated using a combination of immunocytochemistry and transcript analyses of five genes by RT-PCR and 19 genes by droplet digital PCR (ddPCR), whereby a CTC score was calculated. (nature.com)
Using CTC fractions isolated by Parsortix (Angle plc), we investigated the molecular heterogeneity of CTCs isolated from 43 blood samples from metastatic melanoma patients. (nature.com)
But they didn't grow into larger metastatic tumors. (cancer.gov)
Thus MCT models are particularly useful, according to Science Advances, for developing therapies that target metastatic cells. (corning.com)

Amplification of DNA samples with the L1 gene from generic primers were detected in skin biopsies and blood samples from canines healthy clinically or not. (fiocruz.br)
The following protocol was developed in collaboration with Hubrecht Organoid Technology for the culture of Wnt-independent colorectal tumor biopsies in IntestiCult™ OGMH. (stemcell.com)
Spheroids are an important part of this strategy, as they can be grown from cells harvested from tumor biopsies. (corning.com)
A study published in Anticancer Research on head and neck cancers charted the development of a method for generating MCTS from tumor biopsies, the first step toward creating a method of assessing individual susceptibility to chemotherapy drugs and radiation therapy. (corning.com)

Then, orthotopic implantation of breast tumors was performed and tumor growth was followed using bioluminescence. (bmj.com)

We also provide an in-depth protocol for the generation of head and neck squamous cell carcinoma organoids and their subsequent use in semi-automated therapy screens. (nature.com)

Circulating tumour cells (CTCs) can be assessed through a minimally invasive blood sample with potential utility as a predictive, prognostic and pharmacodynamic biomarker. (nature.com)
Circulating tumour cells (CTCs), which are responsible for haematogenous spread of tumours, also reflect this heterogeneity. (nature.com)
The heterogeneous expression of markers, together with their rare frequency (1-10 CTCs in 8 mL of blood) are the main intrinsic factors hampering CTC isolation and limiting in-depth study of these cells in patients with melanoma. (nature.com)
In this study, we compared the recovery and purity of melanoma cells isolated through different microfluidic devices, Parsortix (Angle plc, Surrey, United Kingdom) and ClearCell® FX1 system (Biolidics, Singapore), which enrich CTCs based on their differential cell size, density and deformability. (nature.com)
The isolated CTCs are then cultured in tissue culture plates pre-coated with antibodies which capture the secreted protein of interest. (medscape.com)
CTCs in the blood sample are enriched by negative selection using anti-CD45 immunomagnetic beads. (medscape.com)
The group found that the best patterns to capture CTCs while allowing white blood cells to pass through were a circular pattern with a pitch of 20 micrometers and a diameter of 8 micrometers. (nagoya-u.ac.jp)
Circulating Tumour Cells (CTCs) and circulating tumour DNA (ctDNA) are the major players in liquid biopsy analysis, since they can both provide an alternative approach that enables a sensitive, dynamic and specific serial tumour sampling during the course of treatment, and an early marker of response to systemic therapy [ 1 - 3 ]. (oncotarget.com)

However, these drugs have little effect on brain metastasis due to the combined effects of poor penetration of the blood-brain barrier and their removal from the central nervous system (CNS) by the p-glycoprotein (Pgp) drug efflux pump. (jcancer.org)
Sertoli cell tumors and seminomas rarely exhibit regional or distant metastasis, though local invasion is common. (acvr.org)
In another set of mouse experiments that tracked the timeline of melanoma metastasis to the brain, the team found that individual melanoma cells lacking amyloid beta could spread to the brain and survive there for about a week. (cancer.gov)
Collagen is the most abundant fibrous protein found in mammals' ECMs, and it plays a critical role in tumor growth and metastasis because it promotes cell adhesion and migration. (corning.com)
The patient will require systemic chemotherapy since transitional cell carcinoma always presents with metastasis in those under age 20 years. (medscape.com)

Our results further indicate that MCT4 promotes proliferation and survival by altered cell cycle regulation and cell death mechanisms. (hindawi.com)
Of interest, MM-derived MVs were enriched with the biologically active form of CD147, a transmembrane molecule previously shown by us to be crucial for MM cell proliferation. (oncotarget.com)
In summary, this study demonstrates the significance of MV shedding and MV-mediated intercellular communication on malignant plasma cell proliferation, and identifies the role of MV-enriched CD147 in this process. (oncotarget.com)
Recombinant rat M-CSF induces the proliferation of M-NFS-60 cells in a dose dependent manner. (biolegend.com)
M-CSF is constitutively present at biologically active concentrations in human serum, and binds CD14 + monocytes, and promotes the survival and proliferation of peripheral blood monocytes. (biolegend.com)
ED 50 = 2.5 - 12.5 ng/mL, corresponding to a specific activity of 0.8 - 4.0 x 10 5 units/mg, as determined by induction of N-MSF-60 mouse myelogenous leukemia lymphoblast cell proliferation. (biolegend.com)

Besides, Niraparib activated the cGAS-STING pathway, increasing the levels of cytokines such as CCL5 and CXCL10, which played a vital role in augmenting the infiltration and activation of cytotoxic T cells. (biomedcentral.com)
In our study, we investigated the dynamic changes and responses of immune cells and cytokines during Niraparib treatment. (biomedcentral.com)
Besides, the effects of different T cell functional modulators and cytokines on TIL manufacture have also been examined. (biomedcentral.com)

No differences in the average levels of TNF-α secretion by peripheral blood monocytes or alveolar macrophages were observed. (ersjournals.com)
Flow cytometric analysis of the tumour DNA content showed an index of 1.8 relative to normal peripheral blood lymphocyte DNA. (tmu.edu.tw)

One of the primary reasons for restricted drug access to the CNS is the blood-brain barrier (BBB), which limits the exposure to the CNS and, therefore limits the effectiveness of pharmacological agents in neuronal tissue. (jcancer.org)
The goal of this study was to look for correlates between RNA expression in lung tissue and in whole blood after MWCNT exposure. (cdc.gov)
Analysis of lung tissue RNA using Ingenuity Pathway Analysis (IPA) indicated trends in inflammatory response and connective tissue organization markers, with an emphasis on immune cell trafficking, phagocytosis, and adaptive immune responses. (cdc.gov)
Warm a tissue culture-treated 24-well plate in a 37°C incubator for at least 2 hours. (stemcell.com)
It was observed that tumor cells strongly accelerate plasma coagulation as a result of: i) expression of the blood clotting initiator protein, a tissue factor, as shown by flow cytometry and functional assays (factor Xa formation in the presence of cells and factor VIIa), and ii) direct activation of prothrombin to thrombin by cells, as evidenced by hydrolysis of the synthetic substrate, S-2238, and the natural substrate, fibrinogen. (scielo.br)
Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows . (wikipedia.org)
After the cells of interest have been isolated from living tissue , they can subsequently be maintained under carefully controlled conditions. (wikipedia.org)
In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes , especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture , fungal culture, and microbiological culture (of microbes ). (wikipedia.org)
The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
[6] In 1885 Wilhelm Roux removed a section of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the basic principle of tissue culture. (wikipedia.org)
Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
It is found in large quantities in glioblastoma cells, but is virtually undetectable in healthy brain tissue. (dkfz.de)
The establishment and characterisation of paired autologous tumour cell line (MST-1) and tumour-infiltrating lymphocyte (TIL) culture from a tumour mass of a 14-year-old Taiwanese girl with soft tissue melanoma are described. (tmu.edu.tw)
However, the progenitor cells consist of T cells isolated from resected tumor tissue. (msdmanuals.com)
In clinical applications, which is of most relevance here, each slide corresponds to a tissue or blood sample, e.g. a biopsy. (lu.se)

To find new, more effective treatment options for those affected, doctors and scientists are testing numerous immunotherapeutic approaches, including so-called "adoptive" T-cell therapies: This involves isolationg T cells from the patient, modifying them in the culture dish and transferring them back to the patient. (dkfz.de)
Much more advanced in clinical development than T-cell receptor transgenic cells are cellular therapies using so-called CAR-T (chimeric antigen receptor) cells, which have already been approved for the treatment of various leukemias and lymphomas. (dkfz.de)
Genetically engineering red blood cells to turn them into drug-delivery vehicles could open the door to a vast number of new therapies. (technologyreview.com)
And since mature red cells don't carry any genetic material, they would also carry fewer safety risks than other gene and cell therapies. (technologyreview.com)
In addition to the PKU therapy, Rubius has developed over 50 different therapies based on red blood cells, says Avak Kahvejian , the company's CEO and a partner at Flagship. (technologyreview.com)
Before they become fully mature, mammalian red blood cells eject their genetic material, so as a therapy they are easier to control and less risky than other stem cell and gene therapies, which can lead to abnormal cell growth and tumors. (technologyreview.com)
Human red blood cells circulate for as long as four months, meaning they could potentially form the basis of long-term therapies. (technologyreview.com)
The company is remaining tight-lipped about what additional therapies might be in the pipeline, but Kahvejian says the potential for new drugs based on red blood cells is "only limited by your imagination. (technologyreview.com)
Clinical trials of LAK cells in humans are ongoing but this approach has not gained widespread use and is generally considered less effective than other cell therapies. (msdmanuals.com)

Bunse's team then isolated from the blood of the vaccinated individuals those T cells that had been activated by NLGN4X and consequently carried a T cell receptor that specifically "recognizes" this glioblastoma-associated antigen. (dkfz.de)
However, chimeric antigen receptor-T (CAR-T) cells are emerging as a potential therapeutic strategy. (bvsalud.org)
Chimeric antigen receptors (CAR) recognize specific proteins on the surface of tumor cells. (msdmanuals.com)
Tests such as QFT-G measure the IFN-g released by sensitized white blood cells after whole blood is incubated with antigen. (cdc.gov)

Altogether, by identifying a pro-angiogenic VEGF/miR-101/EZH2 axis in endothelial cells we provide evidence for a functional link between growth factor-mediated signaling, post-transcriptional silencing, and histone-methylation in the angiogenesis process. (plos.org)
Moreover, we found that glioblastoma cells, notorious for their VEGF production, elicited a similar response when co-cultured with endothelial cells [11] . (plos.org)

Document #DX21423), which includes a materials list and instructions for isolating human colonic crypts from biopsy samples, establishing human intestinal organoids from the isolated crypts, as well as expanding and maintaining organoid cultures via passaging. (stemcell.com)
This includes the development of novel proteomics methodologies, identification of liquid biopsy signatures and the molecular identification of novel cell surface markers. (stanford.edu)
MSC increases success rate of biopsy-derived PDO culture, accelerates PDO growth, and promotes monocyte survival and differentiation into tumor-associated macrophages. (bvsalud.org)
Pleural fluid analysis biopsy and lavage has increased the diag- was performed for protein concentration, nostic rate, the cause for many patients with lactic dehydrogenase (LDH), cultures, as exudative PE remains unknown or obscure. (who.int)

In clinical patient samples, Niraparib augmented cytotoxic CD8 + T cell proportion and function. (biomedcentral.com)
Background The success and limitations of current immunotherapies have pushed research toward the development of alternative approaches and the possibility to manipulate other cytotoxic immune cells such as natural killer (NK) cells. (bmj.com)
T-cell engagers are bispecific antibodies that recruit cytotoxic T cells to kill tumor cells. (msdmanuals.com)

A greater number of alveolar macrophages produced TNF-α compared to autologous monocytes in sarcoidosis but not in controls and, based on cell size, it was confirmed that this was the product of more mature macrophages and that the secretion of TNF-α by monocytes and macrophages was heterogeneous: not all monocytes and macrophages secrete TNF-α. (ersjournals.com)
However, AFM24 engages CD16A on NK cells and macrophages with a higher affinity than monoclonal antibodies, causing NK cell-mediated antibody-dependent cellular cytotoxicity and macrophage-mediated antibody-dependent cellular phagocytosis. (onclive.com)
M0, M1, or M2 macrophages were co-cultured with CMFDA-labeled tumor cells in the presence of increasing concentrations of AFM24. (onclive.com)
Finally, M0 macrophages were co-cultured with pHRodo™-labeled tumor cells in the presence of 10 µg/mL of AFM24 or cetuximab. (onclive.com)

Compared to conventional PDOs, MSC-PDO-PBMC and CAF-PDO-PBMC models show comparable responses to chemotherapeutic or targeted anti-tumor drugs but more precise prediction potential in assessing patients' responses to anti-PD-L1 drugs. (bvsalud.org)
EGFR-CAR-T and B7H3-CAR-T cells demonstrated specific anti-tumor activity. (bvsalud.org)

As the research group leader at the Institute for Clinical Chemistry and Clinical Pharmacology at the University Hospital Bonn explains, it was only the advent of the CRISPR-Cas9 gene editing that made it possible to understand the importance of the TLR8 immune sensor in human cells. (uni-bonn.de)
However, some mice experienced dangerous levels of brain swelling, a side effect of the immune response triggered by the engineered cells, the researchers said, adding that extreme caution will be needed to introduce the approach in human clinical trials. (stanford.edu)
similar anti-GD2 CAR-T cells are now being tested in clinical trials in a few other cancers. (stanford.edu)
The company has so far tested the PKU drug in animals and in human blood in the lab, and it aims to begin clinical testing next year. (technologyreview.com)
Also, the term "primary cutaneous CD4 + small/medium T-cell lymphoma" was changed to "primary cutaneous CD4 + small/medium T-cell lymphoproliferative disorder" because of its indolent clinical behavior and uncertain malignant potential. (medscape.com)

This study aimed to improve the function of CAR-T cells through knock down immune checkpoints and immunosuppressive molecular receptors. (bvsalud.org)

These cells can be extracted from whole blood using ficoll, a hydrophilic polysaccharide that separates layers of blood, and gradient centrifugation, which will separate the blood into a top layer of plasma, followed by a layer of PBMCs (buffy coat) and a bottom fraction of polymorphonuclear cells (such as neutrophils and eosinophils) and erythrocytes. (wikipedia.org)
Indeed, footprints of JC polyomavirus and Merkel cell polyomavirus have been detected in PBMCs from pregnant women and women affected by spontaneous abortion. (wikipedia.org)
In many cases, PBMCs are derived from blood banks. (wikipedia.org)

In modern Brazilian herbal medicine practices, Suma root is employed as a cellular oxygenator and taken to stimulate appetite and circulation, increase estrogen production, balance blood sugar levels, enhance the immune system, strengthen the muscular system, and enhance memory. (shamansgarden.com)
The innate cell engager AFM24 showed greater efficacy in eliciting antibody-dependent cellular phagocytosis of EGFR wild-type and KRAS-mutant tumor cells compared with cetuximab. (onclive.com)
The innate cell engager ® (ICE) AFM24 showed greater efficacy in eliciting antibody-dependent cellular phagocytosis of EGFR wild-type and KRAS -mutant tumor cells compared with cetuximab (Erbitux), according to preclinical findings from a study presented in a poster during the 2021 SITC Annual Meeting . (onclive.com)
AFM24 enhances macrophage-mediated antibody-dependent cellular phagocytosis of various EGFR-expressing tumor cell lines, irrespective of the EGFR signaling pathway," wrote lead study author Sheena Pinto, PhD, translational project leader at Affimed, and coauthors, in the poster. (onclive.com)
In this analysis, investigators evaluated the ability of AFM24 to elicit antibody-dependent cellular phagocytosis in solid tumor cell lines expressing EGFR wild-type or EGFR signaling pathway mutations. (onclive.com)
Additional results showed that AFM24 increased antibody-dependent cellular phagocytosis of EGFR -positive tumor cells regardless of macrophage subtype. (onclive.com)
TIL therapy is a type of adoptive cellular therapy leveraging the patient's own immune system to treat tumors. (biomedcentral.com)

Also, M-CSF induces VEGF in monocytes in human tumors. (biolegend.com)

The reverse haemolytic plaque assay allows the measurement of cytokine production at a single cell level. (ersjournals.com)
The current authors have previously demonstrated, using a reverse haemolytic plaque assay (RHPA), that single-cell production of cytokine can be assayed 16 . (ersjournals.com)
Major ovarian tumor cells had been cultured for just two times before useful for practical assay. (bioskinrevive.com)

The cell enters a state of emergency, places itself in a kind of quarantine and produces inflammatory messengers that attract and activate other immune cells. (uni-bonn.de)
These immune cells can then kill infected cells or form antibodies against the pathogens and thus, ideally, fight off the infection in the long term. (uni-bonn.de)
The primary immune cells from these patients enabled the Bonn researchers to validate the results from the CRISPR-Cas9 model cell lines very well," says Prof. Dr. Jutta Gärtner, Director of the Department of Pediatrics and Adolescent Medicine at the University Medical Center Göttingen, who first described this disease and provided the Bonn researchers with immune cells from these rare patients. (uni-bonn.de)
Crystal Mackall says she is encouraged that the work in mice showed that engineered immune cells called CAR-T cells were able to eradicate the tumors in mice. (stanford.edu)
ICE molecules are made to boost the activity of innate antitumor immune cells. (onclive.com)
Our results showed that Niraparib could upregulate PD-L1 expression, but did not impair the function of immune cells, especially CD8 + T cells. (biomedcentral.com)
Mesenchymal stromal cells (MSC) accumulate in primary site to support tumor growth and recruit immune cells to form TME. (bvsalud.org)

In the present study, we dissected the molecular mechanisms responsible for the procoagulant activity of MV3, a highly aggressive human melanoma cell line. (scielo.br)

In mouse and rat xenograft tumor models, TAK-285 showed antitumor activity against cancers that expressed HER2 or EGFR. (jcancer.org)

Tumour necrosis factor (TNF)-α is thought to be a key early cytokine in the pathogenesis of sarcoidosis, despite conflicting data. (ersjournals.com)
Tumour necrosis factor (TNF)-α is a key early cytokine in a variety of chronic inflammatory responses, including fibrosing alveolitis and granulomatous disease 6 - 8 . (ersjournals.com)

Monocarboxylate transporter 4 (MCT4, SLC16A3 ) is elevated under hypoxic conditions in many malignant tumors including gliomas. (hindawi.com)
Malignant gliomas are the most common primary malignant brain tumors with an increasing incidence of up to nine per 100,000 habitants over the last years [ 1 , 2 ]. (hindawi.com)
Multiple myeloma (MM) is characterized by the clonal expansion of malignant plasma cells within the bone marrow. (oncotarget.com)
All patients were given a clini- common ways of establishing a diagnosis cal examination, chest radiograph, blood of tuberculous PE or malignant PE [10-12]. (who.int)

Additionally, gene expression changes in the blood were indicative of ER-stress and downregulation of mTOR pathway. (cdc.gov)
In summary, our data highlight MCT4 /SLC16A3 as a key gene for distinct hallmarks of tumor malignancy in glioma cells. (hindawi.com)
Mouse T cell lymphoma range RMA/B7H6 melanoma cell range B16F10/B7H6 ovarian tumor cell line Identification8/B7H6 were produced by retrovirus transduction of their parental range RMA B16F10 or Identification8 cells respectively using dualtropic retroviral vectors including the human being gene Doripenem Doripenem Hydrate Hydrate relating to protocols previously referred to (17). (bioskinrevive.com)
Mechanistically, EPB41L4A-AS2, acting at least in part as a tumor suppressor, upregulated tumor suppressor gene expression. (cancerindex.org)
However, since only a few NLGN4X-specific T cells can be obtained in this way, which are not sufficient for cell therapy, the scientists resorted to a trick: they isolated the gene coding for the NLGN4X-specific T cell receptor. (dkfz.de)
Global gene expression of Cish fl/fl Ncr1 Ki/+ NK cells compared with Cish +/+ Ncr1 Ki/+ NK cells revealed upregulation of pathways and genes associated with NK cell cycling and activation. (bmj.com)
The main emphasis is on supervised machine learning methods for classification and prediction of tumor gene expression profiles. (lu.se)

To begin the research, the scientists screened human DIPG tumor cultures for surface molecules that could act as targets for CAR-T cells. (stanford.edu)

Cells are embedded in hydrogels to simulate the in vivo extracellular matrix. (sigmaaldrich.com)
Though these manufactured supports are less like the in vivo ECM, they may enhance reproducibility and facilitate cell retrieval from the culture. (sigmaaldrich.com)
The microvessels possess the characteristic morphological and biochemical markers of in vivo blood vessels, and exhibit strong barrier function and long-term stability. (rsc.org)
Hydrogel-based ECMs offer a variety of properties that allow researchers to scaffold MCTS and improve their similarity to in vivo tumors. (corning.com)
The study's results confirmed previous findings that a treatment protocol of cisplatin and radiation was superior to radiation alone and confirmed the feasibility of using primary tumor cells for spheroid formation, which could yield MCTS that more closely mimic in vivo tumor behavior. (corning.com)
Finally, the PTG-T16R-scFv-CAR-T cells showed greater inhibitory effect on tumor growth in vivo, and were superior in prolonging the survival of mice. (bvsalud.org)

Identification of multipotent luminal progenitor cells in human prostate organoid cultures. (nature.com)
Using established molecular biology techniques, Rubius's scientists can engineer progenitor cells taken from human bone marrow and grow blood cells that produce specific therapeutic proteins on their surface or inside the cell. (technologyreview.com)

Nature Cell Biology. (knaw.nl)

Changes in lung and blood transcriptomes following exposure to multiwalled carbon nanotube s in mice. (cdc.gov)
In conclusion, our studies have shown that transcriptome changes occurring in the lungs of MWCNT-exposed mice do not necessarily produce a completely replicable pattern in the whole blood, however, specific systemic responses may be shared between transcriptomic profiles. (cdc.gov)
The study , which was published online April 16 in Nature Medicine, represents the first time a severe human brainstem tumor, diffuse intrinsic pontine glioma, has been eradicated in mice. (stanford.edu)
Next, the team tested the GD2 CAR-T cells in mice whose brainstem was implanted with human DIPG tumors, an experimental system that Monje's lab pioneered. (stanford.edu)
ISC projects apply organoid culture and ko mice to injury-inducible vs homeostatic stem cells and symmetric division mechanisms. (stanford.edu)
Treating the mice with drugs that block the protein greatly reduced melanoma cells' ability to survive in the brain. (cancer.gov)
The results, including from tests in mice, seemed to confirm that the melanoma cells could spread to other organs without the protein but that it was needed for the cells to establish tumors in the brain. (cancer.gov)
Results In Cish fl/fl Ncr1 Ki/+ mice, we detected no developmental or homeostatic difference in NK cells. (bmj.com)
He says not-yet-published work from his lab has shown that cells modified to produce an antibody to a specific bacterial toxin render mice resistant to many times the lethal dose of that toxin. (technologyreview.com)

In this study we showed that an B7H6-specific BiTE directs T cells to mediate cytotoxicity and IFN-γ secretion against B7H6+ tumor cells. (bioskinrevive.com)
In addition, M-CSF enhances monocyte functions such asphagocytic activity, microbial killing, and tumor cell cytotoxicity. (biolegend.com)

We then decreased the expression of these 3 proteins on the surface of CAR-T cells, named PTG-scFV-CAR-T cells. (bvsalud.org)
In contrast to TCR T cells, CAR T cells recognize only relatively large proteins on the surface of tumor cells. (msdmanuals.com)

There is a growing literature that tumor cells release biologically active microvesicles (MVs) that modify both local and distant microenvironments. (oncotarget.com)

This process enables tumor cells, including those within the core of the heterogeneous tumor mass to survive, despite being at a distance from blood vessels that supply their metabolic needs [ 16 , 17 ]. (hindawi.com)

Kretzschmar, K. & Clevers, H. Organoids: modeling development and the stem cell niche in a dish. (nature.com)
Cell Stem Cell. (knaw.nl)

Interleukin-2 (IL-2) expanded TILs had the predominant CD8 + phenotype and the capacity to lyse cells of the cultured autologous tumour. (tmu.edu.tw)

If the cultured cells were genetically modified to stop expressing the target sugar, the CAR-T cells no longer worked. (stanford.edu)
A startup aims to treat hard-to-treat diseases with genetically modified blood cells. (technologyreview.com)

Major human ovarian tumor samples were from Dartmouth-Hitchcock INFIRMARY after medical procedures with educated consent. (bioskinrevive.com)

Moreover, MCT4 overexpression enhances cell migration and invasiveness via reorganization of the actin cytoskeleton. (hindawi.com)
Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. (sigmaaldrich.com)
Tetravalent, bispecific innate cell engager (ICE®) AFM24 enhances macrophage mediated tumor cell phagocytosis. (onclive.com)

After the incubation period, the cells are removed and secreted protein 'spots' are detected by immunological techniques and counted, where each spot corresponds to 1 live CTC (Figure 4). (medscape.com)
After the incubation period, cells are washed out and the released protein spots are detected by the incubation with a fluorochrome-conjugated antibody and counted. (medscape.com)
Blood coagulation involves a series of zymogen acti-vation reactions that are mainly performed by enzyma- tic complexes consisting of a serine protease, a protein co-factor and membranes containing anionic phospholipids (1). (scielo.br)
The researchers found that melanoma cells that travel to the brain produce their own supply of amyloid beta and that this protein is necessary for their survival. (cancer.gov)
Here, we targeted an intracellular inhibiting protein 'cytokine inducible SH2-containing protein' (CISH) in NK cells to evaluate the impact on their functions and antitumor properties. (bmj.com)
It takes only a few weeks to grow cells that produce a new protein of interest-a process Kahvejian calls "rapid prototyping. (technologyreview.com)
early secretory antigenic target--6 (ESAT-6) and culture filtrate protein--10 (CFP-10). (cdc.gov)

Melanoma tumour cells exhibit pronounced heterogeneity within a single tumour, amongst concurrent tumours within the same patient and amongst tumours from different patients. (nature.com)
MCTS mimic many of the features that tumors exhibit in the human body, including extracellular matrix (ECM) deposition between cells, gradients in nutrient concentration, and strong cell-cell junctions. (corning.com)

These data suggest that B7H6-specific BiTE therapy can potentially be beneficial for treating various tumors. (bioskinrevive.com)
But the two cell therapy approaches differ in one important respect. (dkfz.de)
Nevertheless, the successful application of TIL therapy currently is still limited to several types of tumors. (biomedcentral.com)
Lodish says animal tests suggest that engineered red blood cells can be a "very potent" therapy for a range of diseases. (technologyreview.com)
This strategy provides an effective and personalized immune cell therapy against cholangiocarcinoma. (bvsalud.org)

Blood sample from the both epithelial infection and the asymptomatic dogs had been collected for molecular analysis. (fiocruz.br)
The findings documented as mucosal and cutaneous tropism, which the PV viral infection not confined only epithelial sites, but also involved intracellular genome of host cell. (fiocruz.br)
The exact biological mechanism(s) by which the BBB limits drug exposure is not clear, but in addition to epithelial tight junctions, enhanced efflux of chemotherapeutic agents out of the CNS space by endothelial cell membrane transporters in brain blood vessels plays a primary role ( 2 ). (jcancer.org)
It was concluded that circulating epithelial cells may be present in patients with benign inflammatory colon which were detectable by CTC assays. (medscape.com)
Highly characterized cryopreserved human colonic organoids and a step-by-step organoid culture protocol for epithelial intestinal organoid differentiation from iPS cells. (sigmaaldrich.com)

Finally, MCT4 inhibition mitigates the induction of angiogenesis, suggesting that MCT4 also plays a crucial role in tumor-related angiogenesis. (hindawi.com)
The significant growth inhibition by the steroid sulfatase and aromatase inhibitors were also observed in the co-culture system. (scirp.org)

However, there has only been one previous functional study investigating MCT4 in glioblastoma (GBM) neurospheres, mainly focusing on tumor growth and survival dependent on the oxygen level [ 14 ]. (hindawi.com)
Preclinical studies suggest that these myokines can suppress the growth of tumor cells. (medicalnewstoday.com)
In animal studies, we can often slow the growth of a tumor, shrink a tumor or prevent tumors from forming. (stanford.edu)
Excess expression of the sugar is caused by the same mutation, known as the H3K27M mutation, that drives the growth of most DIPG tumors, the team found. (stanford.edu)
Herein we present clear evidence that not only do both patient MM cells and human MM cell lines (HMCLs) release MVs, but that these MVs stimulate MM cell growth. (oncotarget.com)
In 1907 the zoologist Ross Granville Harrison demonstrated the growth of frog embryonic cells that would give rise to nerve cells in a medium of clotted lymph . (wikipedia.org)
Furthermore, we knocked-down the expression of transforming growth factor beta receptor (TGFßR), interleukin-10 receptor (IL-10R), and interleukin-6 receptor (IL-6R) of PTG-scFV-CAR-T cells. (bvsalud.org)

Given history, a Sertoli cell tumor is the primary differential diagnosis, with other testicular neoplasms considered less likely. (acvr.org)
In dogs diagnosed with testicular neoplasia, anywhere from 4-20% of cases will have multiple primary tumors, as seen in this case. (acvr.org)
We previously showed that VEGF signaling in primary endothelial cell cultures caused overexpression of VEGFR2 in a positive feed-forward loop, which is at least partly regulated by loss of miRNA-mediated control of VEGF receptor degradation. (plos.org)
It was assumed by the primary oncologist that the blast cells could have infiltrated the CNS and be present in the brain, although none were noted in the blood. (karger.com)
The cells start their journey by leaving the primary tumor . (cancer.gov)
Finally, we targeted CISH in human NK-92 or primary NK cells, using a technology combining the CRISPR(i)-dCas9 tool with a new lentiviral pseudotype. (bmj.com)
CISH deletion favors NK cell accumulation to the primary tumor, optimizes NK cell killing properties and decreases TIGIT immune checkpoint receptor expression, limiting NK cell exhaustion. (bmj.com)
Finally, using CRISPRi, we then targeted CISH in human NK-92 or primary NK cells. (bmj.com)
Direct comparison study of DNA methylation markers in EpCAM-positive circulating tumour cells, corresponding circulating tumour DNA, and paired primary. (oncotarget.com)
Among the changes to CTCL classification were the addition of primary cutaneous acral CD8 + T-cell lymphoma as a new provisional entity. (medscape.com)

Anatomy21

Organisms11

Diseases79

Chemicals and Drugs50

Analytical, Diagnostic and Therapeutic Techniques and Equipment51

Phenomena and Processes49

Disciplines and Occupations1

Information Science4

Health Care6

Solid tumors5

Vitro23

Antigens12

Brain tumor7

Immunotherapy7

Microenvironment8

Cancer84

Tissues7

Mononuclear8

Lymphocytes5

Circulating Tumor Cells1

Metastatic4

Biopsies4

Breast tumors1

Head and neck squamous cell carc1

CTCs8

Metastasis5

Proliferation6

Cytokines3

Peripheral2

Tissue17

Therapies9

Antigen4

Endothelial cells2

Biopsy4

Cytotoxic3

Macrophages4

Anti-tumor2

Clinical5

Receptors1

PBMCs3

Cellular7

Monocytes1

Assay3

Immune cells7

Melanoma cell line1

Xenograft1

Necrosis2

Malignant4

Gene7

Molecules1

Vivo6

Progenitor cells2

Biology1

Mice9

Cytotoxicity2

Proteins on the surface2

Biologically active1

Heterogeneous1

Stem cell2

Autologous1

Genetically modified2

Human ovarian1

Enhances3

Protein7

Exhibit2

Therapy5

Epithelial5

Inhibition2

Growth7

Primary10