• The aim of this study was the evaluation of the first commercially available in-vitro diagnostic (IVD)- mass spectrometric immunosuppressant assay from Chromsystems (MassTox Immunosuppressants ONEMINUTE Test) and the comparison to a routinely used online solid phase extraction liquid chromatography-tandem mass spectrometric assay method for the measurement of cyclosporine A, everolimus, sirolimus, and tacrolimus in patient whole blood samples. (nih.gov)
  • An API 4000 [tandem mass spectrometer (AB SCIEX)] combined with a CTC Pal autosampler (CTC Analytics AG) and a Shimadzu ultra-fast liquid chromatography (UFLC) system were applied for the direct liquid chromatography-tandem mass spectrometric assay analysis using electrospray ionization in positive ion mode. (nih.gov)
  • 2018. Analysis of lanthionine ketimine ethyl ester in mouse serum, whole blood and tissues using ultrahigh-pressure liquid chromatography/tandem mass spectrometry. . (oregonstate.edu)
  • The found substances were also identified with liquid chromatography-tandem mass spectrometry. (erowid.org)
  • Here we describe a liquid-chromatographic method coupled with tandem mass spectrometry (LC-MS/MS) and we present the results of its comparison with a high-performance liquid-chromatographic method with electrochemical detection (HPLC-ECD). (unimi.it)
  • mono-n-butyl phthalate is chromatographically resolved by reverse-phase HPLC, detected by negative ion atmospheric pressure chemical ionization (APCI) tandem mass spectrometry, and quantified by isotope dilution. (cdc.gov)
  • To examine changes in circulating proteins in response to verapamil treatment, the researchers used liquid chromatography-tandem mass spectrometry of blood serum samples from subjects diagnosed with Type 1 diabetes within three months of diagnosis and at one year of follow-up. (sciencedaily.com)
  • Vitamin D levels were assessed using ultra-high-performance liquid chromatography-tandem mass spectrometry and classified as normal, insufficient, and deficient. (news-medical.net)
  • Metabolites were quantified using reversed-phase ultra-high performance liquid chromatography and electrospray ionization tandem mass spectrometry. (cdc.gov)
  • A HPLC-MS method for the simultaneous quantification of fourteen antiretroviral agents in peripheral blood mononuclear cell of HIV infected patients optimized using medium corpuscular volume evaluation. (unito.it)
  • A sensitive and accurate high performance liquid chromatography-mass spectrometric (HPLC-MS) method for the intracellular determination of 14 antiretroviral drugs in peripheral blood mononuclear cells (PBMCs) for HIV+ patients was validated. (unito.it)
  • Di-n-butyl phthalate may be determined by high resolution gas chromatography with an electron capture detector (HRGC/ECD) (EPA 1984b), gas chromatography/mass spectrometry (GC/MS) (EPA 1994a), gas chromatography with a flame ionization detector (GC/FID) (NIOSH 1994), gas chromatography/Fourier transform infrared spectrometry (GC/FTIR) (EPA 1994b), high performance liquid chromatography/mass spectrometry (HPLC/MS) (Coldham et al. (cdc.gov)
  • For all samples, a fully validated high-pressure liquid chromatography procedure with fluorescence detection was used. (erowid.org)
  • The assay is fluorescence-based and monitors the epoxide hydrolase-mediated conversion of (+/-)-benzo[a]pyrene-4,5-epoxide to (+/-)-trans-benzo[a]pyrene-4,5-dihydrodiol, using a high pressure liquid chromatography separation system. (cdc.gov)
  • Thiols were labeled with monobromobimane, separated on high-pressure liquid chromatography, and detected by fluorescence. (uaeu.ac.ae)
  • Considering the substantial differences in concentrations in blood samples taken from various sites in the body and the high levels in some tissues e.g., in liver, we concluded that the influence of postmortem redistribution should be taken into account in the interpretation of toxicological data when an appropriate peripheral sample cannot be obtained or when blood samples are not available because of putrefaction. (erowid.org)
  • No pharmacokinetic assessment was made, and subtherapeutic artesunate and dihydroartemisinin (as well as clindamycin, piperaquine, quinine, and doxycycline) blood concentrations cannot be excluded. (cdc.gov)
  • 20% artesunate and dihydroartemisinin blood concentrations. (cdc.gov)
  • Activities were assessed in lymphocytes from a styrene -exposed worker population but no significant associations between blood concentrations of styrene and epoxide hydrolase activity levels were observed. (cdc.gov)
  • Tuberculosis patient using RIF has been correlated with treatment failure because of low blood drug concentrations. (ui.ac.id)
  • Plasma robenacoxib concentrations were measured using high-performance liquid chromatography-high-resolution hybrid orbitrap mass spectrometry and noncompartmental pharmacokinetic analysis. (bvsalud.org)
  • Thus, MEHP and perhaps 2-EHA and other di-carboxylic acid concentrations in the body were higher in mice or rats than marmosets when the same dose of DEHP was administered (Ito et al. (who.int)
  • CHGA levels were also easily measured directly in serum using a simple ELISA assay after a blood draw, and lower levels in verapamil-treated subjects correlated with better endogenous insulin production as measured by mixed-meal-stimulated C-peptide, a standard test of Type 1 diabetes progression. (sciencedaily.com)
  • Data on body mass index, waist circumference, resting blood pressure, triglycerides, high-density lipoprotein (HDL) and low-density lipoprotein (LDL) cholesterol, total:HDL cholesterol ratio, blood glucose, and glycosylated hemoglobin (HbA1c) were obtained from annual health examinations. (biomedcentral.com)
  • In this study, we have applied an improved assay for the determination of microsomal epoxide hydrolase activity to assess enzymatic levels in human lung, liver, and blood lymphocytes. (cdc.gov)
  • Multivariable logistic regression assay indicated that lower fasting C-peptide and higher dose of UC-MSC correlated with achievement of clinical remission after transplantation. (biomedcentral.com)
  • Materials and Methods: A simpler analytical method was developed and validated to quantify RIF in DBS samples using a high performance liquid chromatography (HPLC). (ui.ac.id)
  • Disclosed are means, methods and compositions of matter useful for treatment of lung inflammation associated with viral and bacterial infections, as well as with systemic inflammation, through administration of umbilical cord blood derived plasma-based compositions. (justia.com)
  • It is chemically stable, has a low vapor pressure, methyl pyrrolidone (NMP) is important due to its widespread use is miscible in water, and is often used as a substitute for more as a solvent. (cdc.gov)
  • These compounds have high vapor pressures with low water solubility and are emitted as gases from solids or liquids. (cdc.gov)
  • Apoptosis and necrosis was induced to peripheral blood mononuclear cell (PBMC) exposed to oxLDL, based on the duration of exposure. (masticlife.com)
  • Clozapine and its two major metabolites, N-desmethylclozapine and clozapine N-oxide were quantified using a high-performance liquid chromatographic method with UV detection in dog plasma following a single dose of clozapine. (nih.gov)
  • It can be coupled to various chromatographic techniques, such as liquid chromatography , thin layer chromatography , gas chromatography , or inductively coupled plasma . (sigmaaldrich.com)
  • Composition analysis for the ethanol extract (PRExt) was executed by high performance liquid chromatography. (hindawi.com)
  • 1998), or high performance liquid chromatography/ion-trap mass spectrometry (HPLC/Ion Trap-MS) (Coldham et al. (cdc.gov)
  • Therefore, in order to contribute to the establishment of an in-house specification for domestic generic drugs, shorten the time of quantifying each ingredient separately, the study was carried out with the aim of developing and validating procedure for the simultaneous quantification of amlodipine besylate, telmisartan and hydrochlorothiazide by high performance liquid chromatography. (medpharmres.com)
  • A procedure for the simultaneous quantification of amlodipine besylate, telmisartan and hydrochlorothiazide in tablets by high performance liquid chromatography has been successfully developed and validated. (medpharmres.com)
  • For the analysis by high-performance liquid chromatography. (mmshops.store)
  • In: "high performance liquid chromatography and its application to. (mmshops.store)
  • A high-performance liquid chromatography analysis was performed to identify the active ingredients of the PFP extract. (arizona.edu)
  • Early detection and good control of blood pressure reduce the risk of complications and mortality from cardiovascular diseases. (medpharmres.com)
  • A liquid-liquid extraction technique was used to extract clozapine and its metabolites from dog plasma. (nih.gov)
  • Whole blood choline (WBCHO) and plasma choline (PLCHO) are promising new markers in cardiac arrest patients and they are under investigation as markers for global tissue ischemia [ 4 - 6 ]. (biomedcentral.com)
  • Both whole blood choline and plasma choline demon-strated a release pattern in patients after cardiac arrest undergoing hypothermia treatment. (biomedcentral.com)
  • To determine the fate of combined drug thiols, we measured thiol levels in plasma, red blood cells (RBC), and peripheral blood mononuclear cells (PBMC) of four patients. (uaeu.ac.ae)
  • Incubation of a volunteer's blood with mesna, WR-1065, or both revealed that cellular uptake of total reducible drug was ∼10% of plasma level for mesna but ∼60% for WR-1065. (uaeu.ac.ae)
  • In one embodiment the invention teaches administration of umbilical cord blood plasma together with pterostilbene, and/or sulforaphane, and/or thymoquinone, and/or Epigallocatechin gallate (EGCG) and/or n-acetylcysteine in an aerosolized manner to patients suffering from COVID-19 associated pulmonary deficiencies. (justia.com)
  • In another embodiment, umbilical cord blood plasma is administered with immune stimulatory agents in order to concurrently inhibit propagation of viral load in the lung while suppressing pulmonary deficiencies. (justia.com)
  • Direct glutathione quantification in human blood by LC-MS/MS : comparison with HPLC with electrochemical detection / I. Squellerio, D. Caruso, B. Porro, F. Veglia, E. Tremoli, V. Cavalca. (unimi.it)
  • Evaluation of a suspected hemoglobinopathy should include electrophoresis of a hemolysate to detect abnormal hemoglobins and quantification of HbA 2 and HbF with column chromatography. (medscape.com)
  • In the present study, the response of blood pressure, pulse rate [12] , and skin blood flow to the CWC test was evaluated to determine the sympathetic response to mental stress in essential hypertensive patients treated with different types of antihypertensive drugs. (scirp.org)
  • Adults with T1D, IAH and severe hypoglycaemia despite structured education in insulin adjustment, +/− diabetes technologies, were randomised to the "Hypoglycaemia Awareness Restoration Programme despite optimised self-care" (HARPdoc, n = 49), a psychoeducation programme uniquely focussing on changing cognitive barriers to avoiding hypoglycaemia, or the evidence-based "Blood Glucose Awareness Training" (BGAT, n = 50), both delivered over six weeks. (nature.com)
  • Severe hypoglycaemia, defined as a blood glucose concentration so low that cognitive function is impaired and third-party assistance required 1 , remains a feared complication of insulin therapy for people with type 1 diabetes (T1D). (nature.com)
  • Benefits of the blood pressure medication verapamil include delayed disease progression, lowered insulin requirements and preservation of some beta cell function. (sciencedaily.com)
  • Patients taking the oral blood pressure medication not only required less daily insulin two years after first diagnosis of the disease, but also showed evidence of surprising immunomodulatory benefits. (sciencedaily.com)
  • To replace that, patients must take exogenous insulin by shots or pump and are at risk of dangerous low blood sugar events. (sciencedaily.com)
  • In 2018, Shalev and colleagues reported the benefits of verapamil in a one-year clinical study of Type 1 diabetes patients, finding that regular oral administration of verapamil enabled patients to produce higher levels of their own insulin, thus limiting their need for injected insulin to regulate blood sugar levels. (sciencedaily.com)
  • However, major benefits of LC-MS/MS are the improved selectivity, precision and accuracy, the higher sensitivity and the unaltered capacity of detection with time in contrast to ECD. (unimi.it)
  • Results Afatoxin was detected in 59.7% of women and was associated with higher risk of persistent detection of any HPV type (OR=3.03, 95%CI=1.08-8.55, P=0.036), HR-HPV types (OR=3.63, 95%CI=1.30-10.13, P=0.014), and HRHPV types not included in the 9-valent HPV vaccine (OR=4.46, 95%CI=1.13-17.58, P=0.032). (aku.edu)
  • Use gas chromatography/mass spectrometry (GC/MS) analysis, and liquid chromatography/mass spectrometry (LC/MS)analysis to find the differences of metabolic profiles between the high sodium level and the low sodium level, and compare the metabolic profiles of A with the metabolic profiles of B at the low and high sodium level. (sdsc.edu)
  • high-pressure liquid chromatography combined with a mass spectrometer technique was used. (biomedcentral.com)
  • Previous liquid chromatography-mass spectrometry (lc-ms) analyses. (mmshops.store)
  • The analytical cohort for this analysis included 67 HIVuninfected women (mean age 34 years) who completed at least two of three annual study visits and had an available blood sample. (aku.edu)
  • We introduce two new dedicated certified reference materials (CRMs) and their analysis by GC using an ionic liquid capillary column, providing unique selectivity & stability. (sigmaaldrich.com)
  • High-resolution electron microscopy was used to investigate the uptake, distribution, and biotransformation of nanoceria in human and murine M1 and M2 macrophages in unprecedented detail. (cdc.gov)
  • 18 A key risk factor for severe hypoglycaemia is impaired awareness of hypoglycaemia (IAH)-loss of subjective awareness of a falling blood glucose in time to take action to avoid a severe episode 16 , 19 . (nature.com)
  • After 1-year follow-up, 40.7% subjects in MSC-treated group achieved the primary endpoint, significantly higher than that in the control arm. (biomedcentral.com)
  • Mean Hcy level was significantly higher in the test group. (who.int)
  • Quantitation of MDMA and MDA levels occurred in blood samples taken centrally right and left heart and main adjacent great vessels and peripherally subclavian and femoral blood. (erowid.org)
  • We assessed trends in blood lead levels in youths and adults with cotinine-verified tobacco smoke exposure by using 10 years of data from the National Health and Nutrition Examination Survey. (cdc.gov)
  • Geometric mean levels of blood lead are presented for increasing levels of tobacco smoke exposure. (cdc.gov)
  • Positive trend tests indicate that a linear relationship exists between smoke exposure and blood lead levels in youths and adults and that secondhand smoke exposure contributes to blood lead levels above the level caused by smoking. (cdc.gov)
  • Youths with secondhand smoke exposure had blood lead levels suggestive of the potential for adverse cognitive outcomes. (cdc.gov)
  • Although this grain contains adequate tryptophan, it also contains high levels of leucine, another amino acid that interferes with the enzymatic conversion of tryptophan to niacin. (medscape.com)
  • Blood mercury levels will be assessed in subpopulations particularly vulnerable to the health effects from mercury exposure including women of childbearing age (ages 16-49). (cdc.gov)
  • We set out to test the hypothesis that pubs from more deprived areas and non food-serving pubs have higher levels of particulate air pollution. (who.int)
  • The results of our study suggest that blood lymphocytes are a useful sentinel cell for epoxide hydrolase activity determinations in individuals, as these measures are relatively stable over time and appear to reflect activity levels in other target organs. (cdc.gov)
  • Blood was sampled for ghrelin, growth hormone (GH), and pancreatic polypeptide (PP) levels. (bmj.com)
  • In maternal PKU patients with poor compliance to dietary treatment, sapropterin dihydrochloride(6R-BH4)may be an option to improve the management of blood Phe levels. (researchsquare.com)
  • HPA) which can lead to mentalretardation and neuropsychologicalabnormalities (Blau 2010).Early diagnosis and adequate metabolic management of blood Phe levels greatly improved the prognosis and quality of life of PKU patients (Blau 2010, Guthrie 1963). (researchsquare.com)
  • More importantly, elevated maternal blood Phe is particularly relatedto the embryogenesis of heart defects (Lenke 1980).These effects may be prevented with proper nutritional supplements and management of blood Phe levels prior to and throughout pregnancy (Widaman 2003, Langendonk 2012).The suggested target range for blood Phe is 120-360μmol/L, as established by the Maternal Phenylketonuria Collaborative Study (MPKUCS) (Koch 2003, Andersson 2013). (researchsquare.com)
  • Additional techniques may involve Western blotting, quantifying urinary contents (protein, glucose), measuring blood urea nitrogen and glucose levels, and real time PCR techniques. (marshall.edu)
  • ADR and noradrenaline (NOR) levels were determined by high-pressure liquid chromatography. (unipi.it)
  • Various meta-analyses have reported that vitamin D levels are associated with mortality, including a higher incidence of stroke, coronary heart disease, and all-cause mortality in vitamin D-deficient individuals. (news-medical.net)
  • Other biological measurements, such as total and high-density lipoprotein (HDL) cholesterol levels and blood glucose levels, were also obtained from the freshly collected blood samples. (news-medical.net)
  • Larger studies have to evaluate the kinetics in detail and the potential prognostic implications of low or high choline levels in cardiac arrest patients. (biomedcentral.com)
  • In a model controlling for other factors, the use of extracorporeal membrane oxygenation was associated with significantly (p 0.05) higher metabolite levels of acrolein, acrylonitrile, ethylene oxide, propylene oxide, styrene, and ethylbenzene. (cdc.gov)
  • marmosets: rodents had higher levels with greater affinity than marmosets. (who.int)
  • Most relevant associations of blood metabolites with the most accepted phenotypes described for the disease. (archbronconeumol.org)
  • Reliable measurement of glycated hemoglobin in frozen blood samples: implications for epidemiologic studies. (ox.ac.uk)
  • The effects of the PFP extract were evaluated by blood pressure measurement. (arizona.edu)
  • In addition to the blood pressure criteria, proteinuria of greater than or equal to 0.3 grams in a 24-hour urine specimen, a protein (mg/dL)/creatinine (mg/dL) ratio of 0.3 or higher, or a urine dipstick protein of 1+ (if a quantitative measurement is unavailable) is required to diagnose preeclampsia. (medscape.com)
  • The concentration of GSH and GSSG in whole blood samples and their ratios is useful indicator of the oxidative stress status and/or disease risk. (unimi.it)
  • The highest concentration was observed for both molecules at the end of the fourth step, with a significant 1.2-fold increase as compared with blood samples collected at basal 2 for ADR and a 2.4-fold increase for NOR. (unipi.it)
  • The best concentration of the resin having the highest effect was substrate-depended. (masticlife.com)
  • Blood samples were collected at predetermined time points for 5 d. (bvsalud.org)
  • Blood samples were collected in the box (basal 1), in the treadmill room (basal 2), and at the conclusion of each step, using an automatic system. (unipi.it)
  • The participants were required to fill out questionnaires, provide blood samples for various measurements, and undergo a clinical examination. (news-medical.net)
  • Blood samples were taken on admission then again when reaching the therapeutic temperature of 33°C and after 12 hours of MTH at 33°C. All samples were stored at -80°C [ 4 ]. (biomedcentral.com)
  • Blood samples for fasting blood glucose and glycosylated haemoglobin ( H b A l c ) were taken after a median delay of 23 h of the onset of symptoms. (docksci.com)
  • DSN: SEE BLOOD AND URINE DATASET NAMES ABSTRACT General Information HISPANIC HEALTH AND NUTRITION EXAMINATION SURVEY, 1982-84 Mexican Americans Cuban Americans Puerto Ricans Description The Hispanic Health and Nutrition Examination Survey (HHANES) was conducted from July 1982 through December 1984. (cdc.gov)
  • PET-derived binding potential values, used to index striatal DAT density, were significantly decreased after methamphetamine, with larger decreases occurring after higher methamphetamine doses. (jneurosci.org)
  • suggesting that they may be permanent, at least in rhesus monkeys given repeated high doses. (jneurosci.org)
  • With high doses (overdose), users have a distorted perception of their body, the environment, and time. (msdmanuals.com)
  • At even higher doses, hallucinations and paranoid delusions may occur, and the sense of detachment from the world intensifies. (msdmanuals.com)
  • Infant outcomes were related to maternal blood Phe management prior to and during pregnancy. (researchsquare.com)
  • Recently, the role of night blood pressure (BP) in relation to cardiovascular morbidity and mortality has raised a great deal of interest ( 1 ). (diabetesjournals.org)
  • To this day, ARDS continues to result in high mortality and high costs related to usually prolonged hospitalization in the intensive care unit (ICU), stressing hospital resources [1]. (justia.com)
  • The epithelial cells of the small intestine produce citrulline, primarily from glutamine and glutamate, which is secreted into the bloodstream which carries it to the proximal tubule cells of the kidney, which extract the citrulline and convert it to arginine, which is returned to the blood. (wikipedia.org)
  • Mesenchymal stromal cells (MSCs) are multipotent cells with high capacity of immunoregulation, which emerged as a promising cell-based therapy for many immune disorders. (biomedcentral.com)
  • 60 years to discover that the blood pressure control mechanism is actually hidden inside the tiny kidney cells. (girlfriendpedia.com)
  • Solid and liquid types of the product were found to inhibit the production of pro-inflammatory compounds, like nitric oxide (NO) and prostaglandin (PG) e2 by lipopolysaccharide (LPS)-activated mouse macrophage-like RAW264.7 cells. (masticlife.com)
  • Pregnant patients with PKU are associated with an increased risk of complications, as elevated Phe in pregnancy is associated withteratogenic effectsand poor birth outcomes (Lenke 1980, Koch 2003).There is high risk of microcephaly, facial dysmorphism, mental retardation, and intrauterine and/or postnatal growth restriction. (researchsquare.com)
  • Working with medical examiner's offices in West Virginia and Kentucky, we have obtained DNA from autopsy blood cards and are examining single nucleotide polymorphisms (SNPs) in cytochrome P450s (CYPs) related to methadone metabolism as a possible explanation for the overdose deaths and as a predictive tool for preventing methadone overdose in patients taking the drug for addiction treatment or cancer pain. (marshall.edu)
  • Various clinical trials [ 3 - 5 ] have demonstrated that the majority of patients need to be treated with multiple antihypertensive agents with distinct mechanisms of action to achieve and maintain blood pressure goals, in which one-third of patients required three or more antihypertensive agents [ 6 ]. (medpharmres.com)
  • We also conducted analogous measurements on two patients who received mesna alone and a volunteer's blood following in vitro treatment. (uaeu.ac.ae)
  • Kidney transplant recipients have higher malignancy prevalence than hemodialyzed patients. (edu.pl)
  • The objective of the study is to identify changes of urinary metabolite profiles associated with different responses to blood pressure to salt. (sdsc.edu)
  • Exposure to a halogenated aniline can result in a number of toxicities including toxicity to the blood, liver and kidney. (marshall.edu)
  • We found a high prevalence of suboptimal vitamin D status in Iranian adults throughout the year, irrespec- tive of latitude and season. (who.int)
  • In conclusion, skin blood flow can be a useful tool to evaluate sympathetic activity and combination therapy with calcium antagonists and angiotensin II receptor blockers were the most useful therapy for suppressing the hemodynamic response to mental stress. (scirp.org)
  • In clinical strdies the emphasis has been on blood glucose values during the first few days after stroke The clinical relevance of the blood glucose level is difficult to assess as the level is modified by many factors such as diabetes, latent diabetic state, timing of blood sampling, intravenous infusions containing glucose, and stress. (docksci.com)
  • Reduction of skin blood flow by mental stress was suppressed in both the angiotensin II blocker group and the combination therapy group compared with the calcium antagonist group. (scirp.org)
  • Although combination therapy two of three active ingredients telmisartan, amlodipine besylate, and hydrochlorothiazide has been shown to provide positive benefits in blood pressure control and patient tolerability, the combination therapy of all three agents have been shown to be effective in moderate to severe hypertension, with a significant additional reduction in blood pressure compared with dual use [ 7 ]. (medpharmres.com)