• Albumin binding affinity test and in vitro control-released assay were performed to screen these BF-30 derivatives and explore the mechanism of anti-tumour effects. (jcancer.org)
  • Surface plasmon resonance (SPR) measurements, plasma stability test and in vitro control-released assay all showed that LBF-14 exerted better druggability compared with the others. (jcancer.org)
  • The in vitro GA release from MSNs-GA exhibited the same antitumor properties as free GA on Caco-2 cells. (springer.com)
  • Aiyegoro OA, Okohy AI (2010) Preliminary phytochemical screening and In vitro antioxidant activities of the aqueous extract of Helichrysum longifolium DC. (springer.com)
  • We examined the effects of statin drugs on in vitro proliferation, migration and invasion of melanoma cells. (biomedcentral.com)
  • The thesis is focused on studying tau aggregate spreading mechanisms and identifying small molecule inhibitors of tau misfolding and aggregation using in vitro aggregation assays, cell and molecular biology, biochemistry and microscopy techniques. (imtm.cz)
  • I am also working on validating small molecules (selected from in vitro MARK4 kinase activity inhibition assay) on inhibiting microtubule affinity regulating kinase 4 (MARK4) mediated tau phosphorylation using a cell line model. (imtm.cz)
  • 2000. Results of the L5178Y mouse lymphoma assay and the Balb/3T3 cell in vitro transformation assay for eight phthalate esters. (cdc.gov)
  • Beyond showing how BMP signaling impacts DIPG, our study also identified the potent antitumor efficacy of Dacinostat for DIPG. (nature.com)
  • Moreover, after antitumor treatment, [ 89 Zr]Zr-DFO-KN035 enabled observational imaging for therapeutic efficacy evaluation, which can help predict patient prognosis. (thno.org)
  • We employed combinatorial drug screening to identify synergistic combination partners to enhance the efficacy of venetoclax in T-PLL patients. (haematologica.org)
  • The ability of lovastatin, mevastatin and simvastatin to inhibit the melanoma cell proliferation was examined using cytotoxicity and apoptosis assays. (biomedcentral.com)
  • The ethanolic extracts of the plant were examined for the presence of bioactive components and their total flavonoid content, with focusing on quercetin detection using thin layer bioautography (TLB) and brine shrimp lethality assay (BSLA) for cytotoxicity. (who.int)
  • The compound has also been found to have a number of significant biological effects, including antitumor, anti-inflammatory, and antibacterial activities. (genengnews.com)
  • Previous researches revealed that BF-30 is a phenylalanine- and lysine-rich polypeptide exhibiting broad spectrum and strong antibacterial properties without drug resistance. (jcancer.org)
  • Antibacterial activity was evaluated by using disc and agar diffusion methods and the anti-cancer effect of the rose flower extract (RE) was examined using MTT assay in lung cancer cell line. (biomedcentral.com)
  • Collectively, we demonstrate for the first time that curcumin has virucidal activity and virtual inhibition against TGEV, suggesting that curcumin might be a candidate drug for effective control of TGEV infection. (genengnews.com)
  • Thus, the plant may act as a potent antimicrobial drug against resistant strains. (biomedcentral.com)
  • 89 Zr]Zr-DFO-KN035 monitored and predicted the site of adverse reactions in antitumor immunotherapy. (thno.org)
  • Under the tumor immunotherapy strategy, various monoclonal antibody drugs are being developed and marketed to eliminate tumor cells by suppressing tumor immune-checkpoint signaling pathways and reactivating antitumor immune responses. (thno.org)
  • Corresponding cultures yielded a strain identified as M . haemophilum at the Netherlands National Institute for Public Health and the Environment (RIVM) by using the Inno-LiPA Mycobacteria v2 reverse line blot assay (Innogenetics, Ghent, Belgium). (cdc.gov)
  • Using cDNA microarray screens and northern blot analysis, a previous study showed that Beclin 1 is upregulated in liver cancer tissues ( 11 ). (spandidos-publications.com)
  • Microtubule binding drugs are of special interest as they have important roles in the modulation of cellular functions and many of them act as anticancer agents. (rgcb.res.in)
  • Results The screening of three drug libraries relevant to known signaling pathways, FDA (Food and Drug Administration)-approved drugs and neuroendocrine factors yielded two major hits, astemizole and ikarugamycin. (bmj.com)
  • 2019). Thus, screening effective immunopotentiator is benefit for aquaculture, which may reduce the use of antibiotics. (peroxidasessignaling.com)
  • Therefore, based on the characteristics of black phosphorus two-dimensional nanomedicine, experimental designs were conducted on its nanosheet preparation, polyethylene glycol modification, and anti-tumor drug loading. (frontiersin.org)
  • Its drug release characteristics perform well in photothermal therapy, indicating that black phosphorus nano drugs can improve the biological safety and applicability of drugs in new tumor targeted therapy. (frontiersin.org)
  • Targeted therapy uses targeted drugs to maintain a high concentration of the drug in the tumor area, improving the killing efficiency of cancer cells while avoiding damage to other normal cells. (frontiersin.org)
  • Whether it's hormone detection, myocardial disease screening, infectious disease diagnostics, or tumor-related antigen analysis, the ADL i1910 delivers exceptional performance and meets the diverse needs of modern healthcare professionals. (biotechnology.report)
  • Increasing amounts of evidence indicate that autophagy is induced by a number of stressors in tumor cells, such as starvation, growth factor deprivation, hypoxia, damage stimulation and therapeutic drugs, and is an important survival mechanism in response to cellular stress ( 9 ). (spandidos-publications.com)
  • Cyclophosphamide (CTX) is a commonly used alkylating anti-tumor drug. (peroxidasessignaling.com)
  • In a continuation of our work probing the anticancer activity of small sulindac libraries, we have prepared and screened a small diversity library of α-methyl substituted sulindac amides in the profen class. (nih.gov)
  • Traditional Chinese medicine and its active ingredients, are ideal screening libraries for antiviral drugs because of their advantages, such as convenient acquisition and low side effects. (genengnews.com)
  • Experimental analysis was also conducted on the characterization ability, drug release, and targeted therapy of nanomedicine. (frontiersin.org)
  • Essential aspects of binding assays include, but are not limited to, the concentration level of reactants or products (see radioactive section), maintaining the equilibrium constant of reactants throughout the assay, and the reliability and validity of linked reactions. (wikipedia.org)
  • As documented in a recent publication in Stem Cell Reports, Their MOS can be used as patient avatars for studies involving direct viral infection, immune cell penetration, and high-throughput therapeutic drug screening, something not achievable with conventional patient-derived models. (genericviabuy.com)
  • Method For immunopharmacological screening campaigns, we built an artificial miniature immune system in which dendritic cells (DCs) derived from immature precursors present MHC (major histocompatibility complex) class I-restricted antigen to a T-cell hybridoma that then secretes interleukin-2 (IL-2). (bmj.com)
  • The concept has now been extended to embrace synthetic lethal drug sensitivity, such as that observed with PARP inhibitors in combination with DNA-damaging chemotherapy in a variety of BRCA defective tumors 10 . (nature.com)
  • Some Mek inhibitors showed promising antitumor activity, although schedules and doses associated with low systemic toxicity need to be defined. (biomedcentral.com)
  • Finally, several clinical trials are currently ongoing using MEK inhibitors in combination with chemotherapeutic drugs (including dacarbazine or paclitaxel). (biomedcentral.com)
  • Twenty-four candidate compounds were selected based on their clinical approval status, literature data, and mechanisms of drug action. (haematologica.org)
  • These findings will assist the development of anti-tumour drugs to treat lung cancer. (oncotarget.com)
  • Notably, the tuberculin skin test result was negative at screening before she began the TNF-αA treatment, but was now positive (20 mm), suggesting mycobacterial infection. (cdc.gov)
  • Notably, in an adsorption assay, curcumin at 40 µM resulted in a reduction in viral titers of 3.55 log TCID50 ml-1, indicating that curcumin possesses excellent inhibitory effects on the adsorption of TGEV. (genengnews.com)
  • Ligand binding assays provide a measure of the interactions that occur between two molecules, such as protein-bindings, as well as the degree of affinity (weak, strong, or no connection) for which the reactants bind together. (wikipedia.org)
  • Ligand binding assays are used primarily in pharmacology for various demands. (wikipedia.org)
  • We demonstrate that integrative analysis of CRISPR screening datasets enables network-based prioritization of prescription drugs modulating viral entry in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by developing a network-based approach called Rapid proXimity Guidance for Repurposing Investigational Drugs (RxGRID). (stanford.edu)
  • The ligand-binding assay methodology quantified the concentration of the hormone in the test material by comparing the effects of the test sample to the results of varying amounts of known protein (ligand). (wikipedia.org)
  • Its low toxicity, high efficiency, and controllable drug release advantages make it have good application effects. (frontiersin.org)
  • Drug delivery systems seem to be a new method for improving drug targeting and release into cells, bringing new opportunities for improving drug efficiency and avoiding potential side effects. (frontiersin.org)
  • Additionally, the algorithm was able to distinguish between cytotoxic and cytostatic effects of drugs and drug-resistant clones that will cause subsequent relapse. (genericviabuy.com)
  • Side effects of concern are infectious diseases, which prompt the need for screening for latent mycobacterial infection before commencing treatment ( 7 ). (cdc.gov)
  • Conclusions These findings underscore the potential utility of this screening system for the identification of immunostimulatory drugs with anticancer effects. (bmj.com)
  • The team of Dr. Shen was able to create a MOS atlas of human respiratory and digestive tissues from patient autopsies and infect them with SARS-COV-2 viruses, followed by drug screening to identify drugs that block viral infection and replication within those tissues. (genericviabuy.com)
  • Furthermore, time-of-addition assays showed that curcumin mainly acted in the early phase of TGEV replication. (genengnews.com)
  • The two-dimensional nano drug carrier has high safety and effectiveness in drug delivery, and its targeted treatment effect on acute T lymphoblastic leukemia cells is obvious. (frontiersin.org)
  • He Q, Shi J (2012) Mesoporous silica nanoparticle based nano drug delivery systems: synthesis, controlled drug release and delivery, pharmacokinetics and biocompatibility. (springer.com)
  • Because of the excellent platform created by DBT in the last 35 years, India is poised as the biotechnology hotspot amongst developing countries. (rgcb.res.in)
  • in Biotechnology from St. Joseph's College, Tiruchirappalli, India. (imtm.cz)
  • I worked as a Project Assistant at divisions of Toxicology and Microbiology, CSIR-Central Drug Research Institute (CDRI), Lucknow, India and as a Visiting Researcher at the Department of Infection, Immunity and Inflammation, University of Leicester, United Kingdom. (imtm.cz)
  • With its advanced technology, extensive assay menu, and impressive throughput, the ADL i1910 provides healthcare professionals with a reliable and efficient solution for a wide range of diagnostic applications. (biotechnology.report)
  • This symposium presents a remarkable opportunity to convene with other researchers, clinicians, and industry leaders to discuss the extraordinary progress and immense potential of new drug therapies and technologies in paving the way for a brighter future in cancer treatment. (nyas.org)
  • Non-steroidal anti-inflammatory drugs (NSAIDs) have a variety of potential indications that include management of pain and inflammation as well as chemoprevention and/or treatment of cancer. (nih.gov)
  • Such advances provide researchers with the ability not only to quantify hormones and hormone receptors, but also to contribute important pharmacological information in drug development and treatment plans. (wikipedia.org)
  • Carbamazepine is a drug of choice for the treatment of partial and tonic-clonic seizures. (researchgate.net)
  • Despite screening, mycobacterial infections have been diagnosed after prolonged treatment in various patients ( 8 ). (cdc.gov)
  • The statins are group of drugs routinely used in the treatment of lipid disorders, including hypercholesterolemia. (biomedcentral.com)
  • MOS can be further integrated with deep learning image analysis for rapid drug testing of clinical biopsies of small and heterogeneous tumors. (genericviabuy.com)
  • Integrative analysis of functional genomic screening and clinical data identifies a protective role for spironolactone in severe COVID-19. (stanford.edu)
  • We use our results to guide a propensity-score-matched, retrospective cohort study of 64,349 COVID-19 patients, showing that a top candidate drug, spironolactone, is associated with improved clinical prognosis, measured by intensive care unit (ICU) admission and mechanical ventilation rates. (stanford.edu)
  • As part of SAE Media Group's leading series of drug discovery conferences, this year's event will not only bring you the key insights you need to develop your cell and gene therapy pipeline by benchmarking against the latest case studies, but it will also give you the opportunity to network with key players throughout the industry. (smgconferences.com)
  • As a direct result of these monumental findings, researchers have continued the advancement of ligand binding assays in many facets in the fields of biology, chemistry, and the like. (wikipedia.org)
  • This low survival may result from the metastasis of cancer cells and arising resistance to drugs. (oncotarget.com)
  • 6 In addition, independent annexin V/Hoechst assays of primary T-PLL cells on NK-Tert stromal support demonstrated modest single-agent activity of ibrutinib ( Figure 1D , Online Supplementary Figure S2A ). (haematologica.org)
  • Flow cytometry results confirmed those obtained using MTT assay. (springer.com)
  • Screening of herbal plants for various therapeutic properties is the hour as it shows promising activity. (biomedcentral.com)
  • Jacob et al [ 9 ] examined the association between use of cholesterol-lowering drugs and colorectal cancer incidence among 132,136 men and women in the Cancer Prevention Study II Nutrition Cohort. (biomedcentral.com)
  • Current or 5 year use of cholesterol-lowering drugs was not associated with colorectal cancer incidence. (biomedcentral.com)
  • Dr. Shen and his team continue to refine and improve the MOS technology and demonstrate its versatility, not only as a physiological model for screening potential personalized treatments, but also for disease studies and a variety of applications of others," said Ali. (genericviabuy.com)
  • A combination of kinetic fluorometric assay and microscopy for the screening of potential anti-tau aggregating agents, 1.vyd. (imtm.cz)
  • Also, there are increasing cases of antibiotics resistance among most causative agents of diseases in human beings, which calls for an alternative drug discovery using natural sources. (frontiersin.org)
  • The resultant product is no longer sensitive to aminoglycoside-modifying enzymes, a strategy that antibiotic-producing species employ to counter drug resistance in competing species. (hokudai.ac.jp)
  • However, the lack of ideal immunosuppressive models has limited the screening and evaluation of immunopotentiator in fish. (peroxidasessignaling.com)
  • Historically, ligand binding assay techniques were used extensively to quantify hormone or hormone receptor concentrations in plasma or in tissue. (wikipedia.org)
  • Key Features of the Anbio ADL i1910 Extensive Assay Menu: The ADL i1910 boasts an impressive range of 49 developed assays, allowing for both qualitative and quantitative testing of analytes. (biotechnology.report)
  • The foundations for which ligand binding assay have been built are a result of Karl Landsteiner, in 1945, and his work on immunization of animals through the production of antibodies for certain proteins. (wikipedia.org)