Biotechnology bacteria corynebacterium glutamicum. Medical search. Frequent questions

The region comprising orf1 and orf2 displayed a strikingly low G+C content and was present exclusively in C. glutamicum strains. (uni-bielefeld.de)
Study was conducted to enhance lysine production by Corynebacterium glutamicum, strains MTCC1815 and MTCC 2745. (thapar.edu)
When present in mixtures with glucose, the recombinant C. glutamicum strains co-utilized D-galacturonate with glucose and D-glucuronate with glucose, respectively. (springeropen.com)
More recently, I have also initiated studies on bacterial diversity and heterogeneity, working on various bacteria strains alone, in consortia or in interaction with non-equivalent monoclonal subpopulations. (toulouse-biotechnology-institute.fr)

Heterologous expression of cyanophycin synthetase allows production of cyanophycin in a number of biotechnologically relevant bacteria such as Escherichia coli and Corynebacterium glutamicum. (wikipedia.org)
RP4-mediated transfer of mobilizable plasmids in intergeneric conjugation of Escherichia coli donors with Corynebacterium glutamicum ATCC 13032 is severely affected by a restriction system in the recipient that can be inactivated by a variety of exogenous stress factors. (uni-bielefeld.de)
Leverage our advanced machine learning tools, ultra high throughput automation, and proprietary bacterial and fungal production hosts-including Escherichia coli , Bacillus subtilis , Pichia pastoris , Saccharomyces cerevisiae , Aspergillus niger , Corynebacterium glutamicum , Yarrowia lipolytica , and many more model and nonmodel host organisms-to search through the vastness of biological design space to improve your products today. (ginkgobioworks.com)
Neither aldohexuronic acid supported growth of C. glutamicum as sole or combined carbon source, although its genome encodes a putative uronate isomerase sharing 28% identical amino acids with UxaC from Escherichia coli . (springeropen.com)
My research concerns predominantly two model bacteria, Lactococcus lactis and Escherichia coli , using a systems biology approach to elucidate the different levels of metabolic regulation and notably genome wide omic regulations. (toulouse-biotechnology-institute.fr)

These results were confirmed by infection assays with the bacteriophage CL31 from Corynebacterium lilium ATCC 15990. (uni-bielefeld.de)

A mutation in the C. glutamicum ltsA gene caused susceptibility to lysozyme, temperature-sensitive growth, and L-glutamate production. (biomedcentral.com)
To study the ltsA gene function in C. glutamicum, we have screened lysozyme-sensitive mutants of C. glutamicum whose sensitivities were complemented by ltsA gene and examined L-glutamate production by these mutants. (biomedcentral.com)
In the case of both E. coli and C. glutamicum, it was shown that the targeted chromosomal integration of genes (partly in higher gene copy numbers) has advantages over plasmid-based expression. (uni-stuttgart.de)
A basic pattern of gene expression and of relative expression levels during different growth phases was obtained for Corynebacterium glutamicum R grown on different carbon sources. (microbiologyresearch.org)
Identification, sequence analysis, and expression of a Corynebacterium glutamicum gene cluster encoding the three glycolytic enzymes glyceraldehyde-3-phosphate dehydrogenase, 3-phosphoglycerate kinase, and triosephosphate isomerase. (microbiologyresearch.org)
Corynebacterium glutamicum that expresses exogenous GAD gene, gadB2 or gadB1 , can synthesize GABA from its own produced Glu. (springeropen.com)
Finally, gadB2 and mutant gadB1 were co-expressed under the optimal promoter and RBS combination, thus converted Glu into GABA completely and improved GABA production to more than 25 g/L. This study provides useful promoters and RBS sequences for gene expression in C. glutamicum . (springeropen.com)
The bacteria isolated from sewage identified as Proteus mirabilis using 16S rRNA gene made up of D-Mannose, D-Fructose, D-Sucrose, and D-Galactose could be identified by Gas liquid Chromatography Mass Spectrometry (GCMS). (microbiologyjournal.org)
My major area of study is RNA dynamics in bacteria to better understand genome-wide post-transcriptional regulations, their role in the regulation of gene expression and their potential for engineering bacteria. (toulouse-biotechnology-institute.fr)

By using this procedure, clones of the restriction-deficient mutant strain C. glutamicum RM3 harboring a plasmid library of the wild-type chromosome were checked for their restriction properties. (uni-bielefeld.de)
Research published in Biotechnology for Biofuels shares the above aims and recent highlights in the journal include the discovery of novel fungal enzymes that help breakdown biomass for bio-refining as well as the engineering of a strain of Corynebacterium glutamicum that can produce bio-gasoline, whilst tolerating harsh industrial processing. (diwou.com)
The Pyrazines occur ubiquitously in nature and can be strain is able to biosynthesize the pyrazines, including 2,3- biosynthesized by several bacteria. (sagepub.com)
L-lysine production improvement: a review of the state of the art and patent landscape focusing on strain development and fermentation technologies[J]. Critical Reviews in Biotechnology, 2019, 39(8): 1031-1055. (cip.com.cn)

2022. "Cg1246, a New Player in Mycolic Acid Biosynthesis in Corynebacterium Glutamicum. (paris-saclay.fr)
Using E.coli cells as an example, it was shown that the aromatic biosynthesis pathway (shikimic acid pathway) in bacteria could be successfully optimised for the production of aromatic amino acids such as L-phenylalanine, L-tryptophan, p-aminophenylalanine and phenylglycine. (uni-stuttgart.de)
Ergothioneine production by Corynebacterium glutamicum harboring heterologous biosynthesis pathways. (hokudai.ac.jp)
Dihydrodipicolinate synthase (DHDPS) is a regulatory enzyme in the lysine biosynthetic pathway and is the first and major rate-limiting enzyme for lysine biosynthesis in plants and bacteria. (canada.ca)
2011 ). Recently, two metabolic routes for de novo biosynthesis of GABA from glucose have been employed in C. glutamicum , both using its own produced Glu as precursor. (springeropen.com)

Microbial biotechnology and biotransformations promise to diversify the scope of the biorefinery approach for the production of high-value products and biofuels from industrial, rural and municipal waste feeds. (springeropen.com)

Microbial diversity produces a massive pool of unique chemicals, which have become a valuable source for innovative biotechnology. (encyclopedia.pub)
Most major advances in microbiology have occurred within the past 150 years, and several important subdisciplines of microbiology have developed during this time, including microbial ecology, molecular biology, immunology, industrial microbiology, and biotechnology. (researchgate.net)

Hi @ OfficeBECouncil, Yes, #bioscience key to developing #biotechnology as a #green process #technology. (binoculas.net)

This is a soil bacterium that can even live on caffeine. (fz-juelich.de)
Soil bacterium with industrial uses. (up.ac.za)
This organism is a well-studied soil bacterium of considerable importance in biotechnology, in particular for the fermentative production of L-amino acids for food and fodder industry. (up.ac.za)
Listeria monocytogenes is a ubiquitous, Gram-positive soil bacterium and a frequent contaminant of processed food products. (uni-ulm.de)

Corynebacterium glutamicum is an important workhorse of research and the biotechnology industry. (fz-juelich.de)
Corynebacterium glutamicum has developed to a major workhorse of industrial biotechnology. (binoculas.net)

C. glutamicum lysozyme-sensitive mutants were isolated after N -methyl- N '-nitro- N -nitrosoguanidine (NTG) mutagenesis by a research group of Kyowa Hakko Kogyo Co., Ltd. (Tokyo, Japan). (biomedcentral.com)
This plasmid, termed pRES806, is able to complement the restriction-deficient phenotype of different C. glutamicum mutants. (uni-bielefeld.de)

Applied Microbiology and Biotechnology. (wikipedia.org)

We are also working on the targeted modification of the metabolic pathways of E. coli and Corynebacterium glutamicum for the production of aromatic compounds, e.g. amino acids such as phenylalanine or tryptophan, violacein and other fine chemicals. (uni-stuttgart.de)
An easily transferable metabolic engineering strategy for access of C. glutamicum to aldohexuronates was developed and applied to growth and production of the amino acids L-lysine and L-ornithine as well as the terpene lycopene from D-galacturonate or D-glucuronate. (springeropen.com)
Metabolic network analysis during fed-batch cultivation of Corynebacterium glutamicum for pantothenic acid production: first quantitative data and analysis of by-product formation. (microbiologyresearch.org)
Carbon-flux distribution in the central metabolic pathways of Corynebacterium glutamicum during growth on fructose. (microbiologyresearch.org)

His master and PhD thesis have dealt with the natural production of vanillin using the bacterium Amycolatopsis sp. (nrw.de)

2020. "Genome-Wide Identification of Novel Genes Involved in Corynebacteriales Cell Envelope Biogenesis Using Corynebacterium Glutamicum as a Model. (paris-saclay.fr)
Genome reduction is a universal phenomenon in symbiotic bacteria and certain other bacteria that inhabit highly specialized habitats [ 3 ]. (biomedcentral.com)

The actinomycetes are gram-positive, aerobic, filamentous, and spore-forming bacteria, with a superior reputation in producing different kinds of metabolites with a broad spectrum of biological activities, including antioxidant, antifungal, antibacterial, and insecticidal activities. (encyclopedia.pub)
Members of this genus are gener- Leucine Threonine dehydratase ally Gram-positive, rod-shaped, endospore-forming, aerobic or facultatively anaerobic bacteria. (sagepub.com)

The effect of mechanical stresses generated by an extreme agitation intensity or a high aeration rate on growth parameters and cell physiology were studied during continuous cultivation of the Gram-positive bacterium Corynebacterium glutamicum. (birmingham.ac.uk)
Chamsart, S, Hewitt, C & Nienow, A 2005, ' The impact of fluid mechanical stress on Corynebacterium glutamicum during continuous cultivation in an agitated bioreactor ', Biotechnology Letters , vol. 27, pp. 693-700. (birmingham.ac.uk)

C. glutamicum is currently used commercially to produce glutamate and other amino acids (L-lysine) and compounds. (up.ac.za)
A non-pathogenic species of coryneform bacteria, Corynebacterium glutamicum, was originally isolated as an L-glutamate producing bacterium and is now used for fermentative production of various amino acids. (biomedcentral.com)
One of the non-pathogenic species of coryneform bacteria, Corynebacterium glutamicum, is now used for industrial production of amino acids. (biomedcentral.com)
Corynebacterium glutamicum is generally regarded as safe and is widely used for the industrial production of Glu, l -lysine (Lys) and other amino acids (Leuchtenberger et al. (springeropen.com)
KARAU A, GRAYSON I. Amino acids in human and animal nutrition[M]// ZORN H, CZERMAK P. Biotechnology of food and feed additives, 2014: 189-228. (cip.com.cn)

Microorganisms of various types exist in all three domains of life (the Bacteria, Archaea, and Eukarya), and they are by far the most abundant life forms on Earth. (researchgate.net)

Plant Biotechnology. (binoculas.net)
Heiko Rischer is Research Team Leader of Plant Biotechnology at VTT Technical Research Centre of Finland Ltd. and Adjunct Professor in Pharmaceutical Biology at the University of Helsinki. (nrw.de)

More than 2.9 Mio tons of L-glutamate, 1.9 Mio tons of L-lysine and several other products are currently provided by using this bacterium. (binoculas.net)

These acidic sugars are naturally consumed by most plant pathogenic bacteria such as Erwinia carotovora , Pseudomonas syringae , and Agrobacterium tumefaciens , but also by E. coli . (springeropen.com)

To enhance GABA production in C. glutamicum , ribosomal binding site (RBS) sequence and promoter were searched and optimized for increasing the expression efficiency of gadB2 . (springeropen.com)

Coryneform bacteria are rod-shaped, non-sporulating Gram-positive bacteria that are widely distributed in nature. (biomedcentral.com)
This kind of RP systems are widely found in bacteria and the mitochondria in plants and animals, which serve many different physiological processes 5 . (nature.com)
In the present study, bioflocculant derived from bacteria are widely explored in bioremediation such as removing heavy metal and inhibiting biofilm formation. (microbiologyjournal.org)

The bacterium likes glucose and temperatures around 30 degrees Celsius as well as a neutral pH value of 7. (fz-juelich.de)

This bacterium is capable of utilizing various sugars as well as organic acids [ 2 ]. (springeropen.com)
Utilizing a two-step encapsulation process, planktonic cells were first encapsulated in poly(vinyl alcohol) to protect the bacteria against the organic solvents used in the second step employing coaxial electrospinning. (uni-bayreuth.de)

However, it is not workers who are toiling away at heavy machines inside them, but tiny bacteria that metabolise and produce an amazing variety of substances. (fz-juelich.de)

At present, the mechanism of L-glutamate excretion of C. glutamicum is remained to be elucidated. (biomedcentral.com)

Recently, efforts with C. glutamicum have shifted from optimizing production processes to also include access to alternative carbon sources. (springeropen.com)

They may be found as members of the normal microflora of humans, where these bacteria find a suitable niche in virtually every anatomic site. (up.ac.za)
We show that elastic and pH-responsive diblock copolymer fibers are a suitable carrier for Corynebacterium glutamicum, i.e., bacteria which are known for their ability to degrade phenol. (uni-bayreuth.de)

Therefore, optimization of promoters and RBS sequences has been applied for protein expression in C. glutamicum . (springeropen.com)
Combinatorial assembly of tac , cspB and sod promoters and lacZ , cspB and sod RBS elements conferred differential expression of two reporters, eGFP and mCherry in pTGR platform of C. glutamicum (Ravasi et al. (springeropen.com)

In recent years, there has been considerable interest in biotechnology and synthetic biology research concerning the design and construction of an optimal chassis cell [ 1 ]. (biomedcentral.com)

I am a scientist in the " Microbiology and Food Chain " division of the French National Institute for Agricultural, Food and Environmental Research, currently working at the Toulouse Biotechnology Institute as head of the research group BLADE (Bacterial Adaptation Diversity and Engineering). (toulouse-biotechnology-institute.fr)
I am interested in the study of Bacterial Physiology and Metabolism to better understand mechanisms of adaptation of bacteria to their environment and to engineer bacteria for applications in biotechnology. (toulouse-biotechnology-institute.fr)

R. opacus is a Gram-positive actinomycete bacterium that can accumulate triacylglycerols (TAGs), a biodiesel precursor, up to ~ 78% of its cell dry weight when grown on sugars [ 25 ]. (biomedcentral.com)

A bio-based economy, that harnesses biotechnology and renewable raw materials to create fuels, chemicals and materials, aims to reduce both the consumption of fossilized carbon and the resulting pollution. (diwou.com)
I recently attended the Symposium on Biotechnology for Fuels and Chemicals , where the keynote speaker, Jennifer Holmgren (CEO of Lanzatech ) set the tone of the meeting with a proposal that we should limit our fossil-based carbon budget to the essentials, and that energy can be carbon free. (diwou.com)
Corynebacterium glutamicum , which is used in industrial amino acid production at a million-ton scale, has been engineered towards utilization of alternative carbon sources. (springeropen.com)
Heterologous expression of the genes for both uptake and catabolism of D-galacturonic acid and D-glucuronic acid was required to enable growth of C. glutamicum with either aldohexuronic acid as the sole carbon source. (springeropen.com)
Carbon catabolite repression in bacteria: choice of the carbon source and autoregulatory limitation of sugar utilization. (microbiologyresearch.org)

Generally, bacteria produces a variety of polysaccharides which are classified based on location such as, intracellular, extracellular, and capsular polysaccharides. (microbiologyjournal.org)

In humans, for example, this intestinal bacterium is responsible for the production of vitamin K. Biotechnologists use the organism to produce fine chemicals, enzymes or drugs, such as insulin. (fz-juelich.de)
C. glutamicum shows high tolerance against lysis by lytic enzymes such as egg white lysozyme that catalyzes hydrolysis of β-1,4 glycoside bond between N -acetylglucosamine and N -acetymuramic acid of peptidoglycan. (biomedcentral.com)

The rapid proliferation of bacteria and abundant EPS in the vascular system will block xylem vessels and disrupt water flow, causing wilt disease symptoms and eventually the death of infected plants ( Genin and Deny, 2012 ). (ppjonline.org)
Utilization of the hexuronic acids D-galacturonic acid (abundant in pectin-rich waste streams such as peels and pulps) and D-glucuronic acid (a component of the side-chains of plant xylans) for growth and production with C. glutamicum has not yet been studied. (springeropen.com)

In bioreactors, research and industry breed different bacteria for different purposes. (fz-juelich.de)

In different projects involving both academic and industrial partners, these sensor bacteria are used to screen larger collections of bacteria, e.g. starter cultures for food production, to identify producers of (novel) bacteriocins. (uni-ulm.de)

Identification and alignment of the promoter regions of the transcriptional start sites of glycolytic genes revealed strong similarities to the σ A consensus promoter sequences of Gram-positive bacteria. (microbiologyresearch.org)
For example, aph promoter combined with different RBS sequences were used for expression of reporter proteins in C. glutamicum (Zhang et al. (springeropen.com)

Protein degradation in this bacterium appears to be triggered by the accumulation of polyP. (go.jp)
Here I propose to call the polyP-Lon complex the "stringent protease," and I discuss new insights of protein degradation control in bacteria. (go.jp)
KIDD M T, MAYNARD C W, MULLENIX G J. Progress of amino acid nutrition for diet protein reduction in poultry[J]. Journal of Animal Science and Biotechnology, 2021, 12(1): 45-45. (cip.com.cn)

Once identified, the bacteriocins produced by these bacteria, their mechanisms of action and receptors can be analyzed and characterized again using the developed sensor bacteria. (uni-ulm.de)

Thus, rapid changes of the intracellular (neutral) pH of the sensor bacteria to the external pH in a more acidic or alkaline environment/buffer are indicative of a disruption of membrane integrity. (uni-ulm.de)

More recent work in the journal shows how bioplastic precursors can also be produced in yeast and bacteria . (diwou.com)

2020. "A Method for Producing Highly Pure Magnetosomes in Large Quantity for Medical Applications Using Magnetospirillum Gryphiswaldense MSR-1 Magnetotactic Bacteria Amplified in Minimal Growth Media. (paris-saclay.fr)
Growth and fermentation of D-xylose by Saccharomyces cerevisiae expressing a novel D-xylose isomerase originating from the bacterium Prevotella ruminicola TC2-24. (scielo.br)
Our data takes the lead in demonstrate that the threonine transporter Rv3737 is required for in vitro growth and survival of bacteria inside macrophages. (biomedcentral.com)
For instance, threonine transporter ThrE is essential for the fitness of Corynebacterium glutamicum in vitro growth [ 8 ]. (biomedcentral.com)

Essentiality of the Maltase AmlE in Maltose Utilization and Its Transcriptional Regulation by the Repressor AmlR in the Acarbose-Producing Bacterium Actinoplanes sp. (frontiersin.org)

For thousands of years, biotechnology has been used as a natural process to produce food such as cheese, bread and beer. (nrw.de)
The Karotec project has the goal to establish Corynebacterium glutamicum as a host to produce carotenoids, especially astaxanthin, for both feed/food and cosmetic applications. (nrw.de)

Biotech News is your website for latest breaking news and videos straight from the biotechnology industry. (diwou.com)
Since its discovery, C. glutamicum has become an indispensable microorganism for the biotechnological industry [ 9 ]. (springeropen.com)

Researchers are thus already trying to sort out the bacteria that react too sensitively to fluctuations on the laboratory scale. (fz-juelich.de)

Our tea-bag like biohybrid system, where the pH-responsive diblock copolymer acts as a protecting outer shell for the embedded bacteria, allows phenol degradation even at low pH. (uni-bayreuth.de)

Organisms8

Diseases1

Chemicals and Drugs4

Analytical, Diagnostic and Therapeutic Techniques and Equipment2

Phenomena and Processes3

Disciplines and Occupations2

Technology, Industry, Agriculture3

Information Science1

Strains4

Escherichia5

ATCC1

Gene9

Strain4

Biosynthesis5

Biofuels1

Microbial2

Bioscience1

Soil4

Workhorse2

Mutants2

Microbiology and Biotechnology1

Metabolic4

Amycolatopsis1

Genome2

Aerobic2

Cultivation2

Amino acids5

Archaea1

Plant Biotechnology2

Lysine1

Coli1

Sequence1

Widely3

Glucose1

Organic2

Substances1

Glutamate1

Production processes1

Suitable2

Promoters2

Considerable1

Bacterial2

Opacus1

Carbon5

Produces1

Enzymes2

Abundant2

Research1

Industrial1

Sequences2

Protein3

Mechanisms1

Acidic1

Journal1

Growth4

Regulation1

Produce2

Industry2

Laboratory scale1

Outer1