AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsInformation ScienceHealth Care
MicrodissectionLaser Capture MicrodissectionLasersDissectionBiological MarkersGene Expression ProfilingHistocytological Preparation TechniquesSperm RetrievalTumor Markers, BiologicalOligonucleotide Array Sequence AnalysisReverse Transcriptase Polymerase Chain ReactionParaffin EmbeddingImmunohistochemistryPolymerase Chain ReactionMicromanipulationRNA, MessengerProteomicsTissue FixationCryoultramicrotomyTissue EmbeddingRNAHematoxylinLoss of HeterozygosityNucleic Acid Amplification TechniquesReproducibility of ResultsGene Expression Regulation, NeoplasticLaser TherapyAzoospermiaDNA, NeoplasmIn Situ Hybridization, FluorescenceChromosome PaintingProtein Array AnalysisIn Situ HybridizationFixativesGene ExpressionRNA, NeoplasmFormaldehydeFrozen SectionsBiopsySensitivity and SpecificityMicroarray AnalysisPyronineEpithelial CellsDisease ProgressionPrognosisProteomeStaining and LabelingBronchiolesMass SpectrometryPrecancerous ConditionsMicrosatellite RepeatsBase SequenceChromatography, LiquidUp-RegulationGene Expression RegulationSpecimen HandlingProstatic NeoplasmsGene AmplificationAdenocarcinomaMolecular Sequence DataNeoplasm ProteinsBreast NeoplasmsMicrosurgeryOrotate PhosphoribosyltransferaseTandem Mass SpectrometryCell Line, TumorSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationCluster AnalysisCase-Control StudiesDNA PrimersChromosome BandingStromal CellsRats, Sprague-DawleyMutationMicrotomyCell SeparationPredictive Value of TestsTestisComputer SystemsCarcinoma in SituTissue Array Analysis
ProgressionProteomicsGenomicPrognosticTumor cellsCandidate biomarkersPredictive biomarkerCancerTherapeuticMethodsLaserMolecularPancreaticEpithelialProteinsMass spectrometry2020MRNAPopulationsTissuesSerumMutationsPathwaysEfficacyIsolateDiagnosticIdentificationApoptosisRenal cell carcGenomeProliferationProteinCirculatingClinical2021Fluorescence-activated cellDetectionDifferentiationMetabolomicMetastasisGeneNSCLCBiologyHER2TumourOverexpressionSamplesGenesExperimentalSpecificProteomeRNAsTumorsIsolationCellularDiscovery
Progression11
  • Lastly, when your goal is to study cellular progression through differentiation, average expression profiles can only order cells by time rather than by developmental stage. (wikipedia.org)
  • UC researchers analyzed the AMACR gene's abnormal expression patterns using a sophisticated laser-capture microdissection technique to identify the key biological events that lead to colon cancer progression. (sciencedaily.com)
  • [ 14 ] Subsequent work in our laboratory demonstrated that K17 serves as a nuclear shuttle of p27KIP1, resulting in sustained cell cycle progression. (medscape.com)
  • Plasma galectin-3 (Gal-3) is associated with organ fibrosis, but whether urinary Gal-3 is a potential biomarker of kidney disease progression has never been explored. (mdpi.com)
  • Since CTCs are accessible from peripheral blood, these cells represent a potential source of highly relevant tumor materials, which could be used to reveal new biomarkers for monitoring disease progression and evaluating drug efficacy. (ubc.ca)
  • These results demonstrate the potential of our single cell sequencing workflow to discover clinically relevant mutations from single CTCs that may aid in monitoring disease progression and guiding treatment. (ubc.ca)
  • In the present study we display that c-kit manifestation by PCa cells supports their migration and invasion and downregulates transgene reduces c-kit protein manifestation and migration and invasion of c-kit-transfected PCa cells suggesting a novel cross-regulation between these two genes that supports PCa progression. (acancerjourney.info)
  • The application of these new proteomic technologies to study cellular activities, will lead to a faster sample throughput and increased sensitivity for the detection of individual proteins, thus providing major opportunities for the discovery of new biomarkers for the early detection of protein alterations associated with the progression of the disease state. (usuhs.edu)
  • We are funded by the NIH for a comprehensive identification of biomarkers correlated to the progression of this disease, through proteomic, lipidomic, and metabolomic characterization of human pancreatic tissues and longitudinally collected serum samples, as well as for a comprehensive validation of the candidate markers in diseases that share the similar clinical and immunological outcomes with T1D. (uncg.edu)
  • Ideally, biomarkers can be used to predict disease progression and treatment response, but these studies will take many years due to the requirement for lengthy follow-up periods to assess outcomes. (biomedcentral.com)
  • This review will focus on the contribution of high-throughput genomic and proteomic approaches to the study of fibrogenesis and fibrosis progression, concentrating on the most prevalent human chronic liver diseases and findings from animal models in liver tissue, isolated liver cells, cell lines and serum. (biomedcentral.com)
Proteomics2
Genomic7
  • A special focus is lying on the integrative analysis of multi level biomarkers, e.g. microRNA - mRNA, integrative analysis of genomic, proteomic, metabolomic and phenotypic markers. (gene-quantification.de)
  • Current challenges in the field are how we share these disruptive technologies to the scientific communities while still including new approaches, such as genomic cytometry and single cell metabolomics. (frontiersin.org)
  • Specifically, we identified DNA methylation differences in intestinal epithelial cell genomic DNA within resected ileum or colon that may provide new insights into the biology and mechanisms of NEC pathogenesis. (biomedcentral.com)
  • The study provides a novel method for identification of cancer biomarkers related to genomic instability and immunotherapy. (biomedcentral.com)
  • These data demonstrate that NT/ICM on nuclear monolayers is a very promising single platform test of genomic instability, which may aid pathologists in the diagnosis of dysplasia and has potential as a biomarker in BO. (nature.com)
  • The aim of this study was to identify genomic aberrations in hepatocellular carcinoma (HCC) by using laser capture microdissection (LCM) combined with microarray analysis. (geneticsmr.org)
  • Current genomic and proteomic research provides many candidate biomarkers, but independent validation of these biomarkers is lacking, and reproducibility is still a key concern. (biomedcentral.com)
Prognostic9
  • Based on the similar pathogenesis of squamous and glandular lesions of the cervix, we hypothesized that K17 overexpression could also be a diagnostic and/or prognostic biomarker for endocervical neoplasia. (medscape.com)
  • [ 16-20 ] There remains a critically important, unmet clinical need, however, to identify prognostic (as opposed to diagnostic) biomarkers of endocervical adenocarcinoma. (medscape.com)
  • Thus, the goal of this study was to determine if K17 expression is a negative prognostic biomarker associated with decreased survival of patients with endocervical adenocarcinoma. (medscape.com)
  • A session about the discovery, identification and validation of molecular biomarkers: diagnostic, prognostic or therapeutic markers on DNA, RNA, microRNA or small RNA, metabolome and proteome level (e.g. disease markers, cancer or stem-cells markers, tissue specific markers, differentiation markers, methylation markers). (gene-quantification.de)
  • Effective, reliable use of molecular biomarkers to inform clinical practice requires the standardisation of testing methods and careful assessment of biomarkers' predictive and prognostic value. (ersjournals.com)
  • Specifically, it is becoming increasingly important to accurately distinguish biomarkers as "prognostic" or "predictive", or define them as both. (ersjournals.com)
  • Prognostic biomarkers can be thought of as a measure of the natural history of a disease that is independent of therapeutic intervention (or lack of it) 5 . (ersjournals.com)
  • A population-based register or a placebo/control group from a randomised clinical study is appropriate for evaluating the prognostic value of a biomarker 5 . (ersjournals.com)
  • In order to establish which patients will benefit most from a treatment, and by how much, the predictive value of a biomarker must be separated from its prognostic value. (ersjournals.com)
Tumor cells6
  • Most surgically removed tissue samples contain a low percentage of tumor cells embedded in a dense stroma. (genengnews.com)
  • Single and pooled tumor cells (SK-BR-3) obtained from EDTA- and CellSave-preserved blood and archival material were whole-genome amplified. (takarabio.com)
  • Single-cell laser microdissection was utilized to identify the minimal number of tumor cells needed for mutation detection. (takarabio.com)
  • 2. Isolation of the HR-and Herceptin sensitive (HS)-tumor cells from the BC tissues for genotyping analysis by laser capture microdissection (LCM). (tmu.edu.tw)
  • The key procedure of anti-tumor respond is the activation of T cell and tumor cells can inhibit this process through binding its immune checkpoints to receptors expressed on T-cell, thus leading to immune escape. (biomedcentral.com)
  • CRC cells secreted extracellular miR-224-5p mainly in an exosome-dependent manner, and then miR-224-5p could be transferred to surrounding tumor cells to regulate cell proliferation in the form of autocrine or paracrine. (besjournal.com)
Candidate biomarkers1
  • Three selected candidate biomarkers (CD59, CD70, and CD74) mediate the evasion of the immune response. (genengnews.com)
Predictive biomarker3
  • HNMT is potentially a predictive biomarker that can help clinicians to more accurately identify responders or nonresponders to improve the therapeutic index for Herceptin® monotherapy. (tmu.edu.tw)
  • Aim-3: HNMT as a predictive biomarker to accurately select responders for Herceptin® Tissue microarray analysis will be performed to determine the following: 1. (tmu.edu.tw)
  • In contrast, a predictive biomarker differentiates a group with a particular response or lack of response to a therapeutic intervention. (ersjournals.com)
Cancer29
  • Thus, little is known about early biomarkers of pancreatic cancer. (genengnews.com)
  • Our team was the first to conduct an in-depth molecular comparison of cancer and normal tissues for the presence of cancer stem cells," says David M. Lubman, Ph.D., professor of surgery at the University of Michigan, Ann Arbor. (genengnews.com)
  • Identification of crucial cancer biomarkers requires analysis of a pure and homogeneous cell population. (genengnews.com)
  • This method allowed us to zero in on a small population of cancer stem cells expressing CD24, a specific cell surface biomarker shown to correlate with the development and metastasis of pancreatic cancer," continues Dr. Lubman. (genengnews.com)
  • Scientists have identified a new biomarker that could help predict a person's risk of developing colon cancer and how aggressive it may become. (sciencedaily.com)
  • Researchers speculate that these hotspots could be used as a biomarker for colon cancer. (sciencedaily.com)
  • This study is the first to explain how a gene-AMACR-is regulated in relation to cancer development and to identify specific genetic events (a polymorphism and somatic cell mutations) related to colon cancer. (sciencedaily.com)
  • Cervical cancer has multiple histologic subtypes, with squamous cell carcinoma (SCC) accounting for approximately 75% of cases and invasive adenocarcinoma accounting for 10% to 15% of cases. (medscape.com)
  • Comparative study of whole genome amplification and next generation sequencing performance of single cancer cells. (takarabio.com)
  • Year-1: Validation of HNMT as a predictive marker for Herceptin-Resistance (HR) in breast cancer patients Aim 1: Determination of the HNMT and HER2 mRNA expression level in BC cells 1. (tmu.edu.tw)
  • The principle of preferentially selecting patients most likely to benefit from therapy according to their genetic profile has led to substantial clinical benefit in some tumour types, and has potential to considerably refine treatment in advanced nonsmall cell lung cancer (NSCLC). (ersjournals.com)
  • This review considers the current evidence linking biomarker profile to efficacy of epidermal growth factor receptor (EGFR)-targeted therapy in advanced nonsmall cell lung cancer (NSCLC) and the clinical implications of recent findings. (ersjournals.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • Oral squamous cell carcinoma (OSCC) is the most common type of head and neck malignant tumor and is ranked the eighth leading cause of cancer worldwide. (nature.com)
  • Using this process, we enriched and isolated 30 single CTCs from 3 patients with metastatic prostate cancer, and sequenced 5 of these single CTCs from a patient with matched cell-free DNA. (ubc.ca)
  • The majority of patients with non-small-cell lung cancer (NSCLC) present with advanced disease, with targeted therapies providing some improvement in clinical outcomes. (bmj.com)
  • Lung cancer is the most prevalent life-threatening cancer worldwide 1 with more than 80% being non-small-cell lung cancer (NSCLC). (bmj.com)
  • Biomarkers can be used as indicators of certain diseases, such as cancer. (leica-microsystems.com)
  • This talk will focus on Manchester's experience of circulating tumour cells and cell-free DNA in lung cancer. (uib.no)
  • This is also keynote lecture at the R&D Network meeting Cancer biomarkers and targeted therapy . (uib.no)
  • Meet the cancer cell in person! (uib.no)
  • CCBIO offers schools a unique way of learning about cancer cells in the form of a play, or a highly entertaining presentation for other audiences. (uib.no)
  • Children and youth are introduced to cancer cells in a very different way! (uib.no)
  • Schools have a choice between the play " Stine Stemcell finds herself " (in Norwegian only) which is appropriate for children/youth in the age of 10 to 14, or the lecture/stand-up routine " Christine the Cancer Cell - A sociopath in the body ", suitable for youth and adults. (uib.no)
  • She is absolutely horrified by the total lack of common decency she has discovered among cancer cells. (uib.no)
  • Henriette also has the play " Stop the cancer cell Gloria Glutton ! (uib.no)
  • We found that piR-FTH1 negatively regulates the Fth1 expression at post-transcriptional level in triple negative breast cancer (TNBC) cells. (omicsdi.org)
  • Here we report the identification of 555 piRNAs in human lung bronchial epithelial (HBE) and non-small cell lung cancer (NSCLC) cell lines, including 295 that do not exist in databases termed as piRNA-like sncRNAs or piRNA-Ls. (omicsdi.org)
  • There is much interest in the utility of molecular biomarkers in BO, both to predict which patients may develop cancer (and therefore offer therapy) and to aid prognostication by guiding surveillance intervals following therapy. (nature.com)
Therapeutic5
  • The early appearance of CD74 means that this candidate biomarker could be a promising tool in therapeutic drug discovery. (genengnews.com)
  • In vitro studies (FRET and FLIM) also demonstrated that HNMT/HER2 complex formation in BC cells may affect therapeutic efficacy. (tmu.edu.tw)
  • These findings might therefore bring about brand-new biomarkers and therapeutic targets for PCa individuals presenting bone tissue metastasis. (acancerjourney.info)
  • 5HN has also been identified to impact several cellular proteins, indicating that despite its ability to activate HIV without widespread T cell activation, it may nevertheless be too toxic for therapeutic use (Yang et al. (nicotinic-receptor.com)
  • Thus, LCM is an effective approach for identifying aberrantly expressed genes in HCC, and may lead to the discovery of biomarkers for diagnostic and therapeutic applications. (geneticsmr.org)
Methods12
  • There is so far no standardized technique to generate single-cell data: all methods must include cell isolation from the population, lysate formation, amplification through reverse transcription and quantification of expression levels. (wikipedia.org)
  • There are several methods available to isolate and amplify cells for single-cell analysis. (wikipedia.org)
  • These methods include: Micropipetting Cytoplasmic aspiration Laser capture microdissection. (wikipedia.org)
  • High-throughput methods are able to quickly isolate hundreds to tens of thousands of cells. (wikipedia.org)
  • Performance of four modern whole genome amplification methods for copy number variant detection in single cells. (takarabio.com)
  • METHODS: We performed a comparative proteome analysis on three Abi sensitive (LNCaPabl, LAPC4, DuCaP) and resistant (LNCaPabl-Abi, LAPC4-Abi, DuCaP-Abi) PCa cell lines using liquid chromatography tandem mass spectrometry (LC-MS/MS) technique. (bvsalud.org)
  • Focus is on micro-genomics, the application of molecular methods to detect biomarkers in a minimal amount of tissue or matrix. (gene-quantification.de)
  • The Zhang research group focuses on the development of new capabilities for more accurate, more sensitive and higher throughput measurement of biomolecules, and the application of the most reproducible methods to characterize clinical specimen at proteomic, lipidomic and metabolomic levels for comprehensive identification of disease biomarkers, and systems biological understanding of the pathogenic mechanism underlying the complex human diseases. (uncg.edu)
  • Methods The miR-224-5p expression in CRC patient tissues and cell-derived exosomes was measured by laser capture microdissection and qRT-PCR, respectively. (besjournal.com)
  • Falk Schlaudraff (Institute of General Physiology of the University of Ulm, Germany) used modern laser microdissection methods to isolate and analyze cells from post mortem tissue specimens taken from M. Parkinson patients in order to gain molecular insight into the disease. (leica-microsystems.com)
  • Transcriptome profiling can be an indispensable tool in advancing the knowledge of one cell biology but is dependent upon methods with the capacity of Gossypol isolating mRNA on the spatial quality of an individual cell. (ecologicalsgardens.com)
  • Existing methods have got been successful at isolating mRNA from populations of living cells including neurons using manual sorting stream cytometry or immunopanning8-10. (ecologicalsgardens.com)
Laser16
Molecular9
  • Previous molecular characterizations of mammary stem cells (MaSC) have utilized fluorescence-activated cell sorting or in vitro cultivation of cells from enzymatically dissociated tissue to enrich for MaSC. (frontiersin.org)
  • Our results support the use of DNA label retention to identify MaSC and also provide a molecular profile and novel candidate markers for these cells. (frontiersin.org)
  • A special BDQ issue with the title "Advanced Molecular Diagnostics for Biomarker Discovery" will be published in summer 2105. (gene-quantification.de)
  • However, the use of molecular biomarkers to optimise clinical outcomes requires careful assessment of their role in terms of both prognosis and treatment decision-making. (ersjournals.com)
  • Different regions of oral squamous cell carcinoma (OSCC) have particular histopathological and molecular characteristics limiting the standard tumor−node−metastasis prognosis classification. (nature.com)
  • Quantifying the degree of BRAF protein manifestation seen in a tumour may consequently be used as a first step to assess for intra-tumoural heterogeneity permitting subsequent intra-tumoural sampling to occur such as microdissection followed by detailed molecular testing. (academicediting.org)
  • Circulating biomarkers may bypass some of these limitations but bring challenges of their own, not least in performing molecular profiling on small amounts of material. (uib.no)
  • The aim of our study was to determine the molecular phenotype of NEC via transcriptomic and epithelial cell-specific epigenomic analysis, with a specific focus on DNA methylation. (biomedcentral.com)
  • Nevertheless cells of apparently the same type aren't identical on the molecular level1-3 and demonstrate a differing amount of heterogeneity amongst their portrayed mRNAs and proteins which may be influenced by mobile stimulation. (ecologicalsgardens.com)
Pancreatic3
  • Therefore, establishing specific biomarkers is important for diagnosis and prognosis evaluation of pancreatic adenocarcinoma. (biomedcentral.com)
  • Studies report that detection of germ cell mutations, somatic mutations and fusion, and microsatellite instability (MSI)/mismatch repair defects in pancreatic adenocarcinoma play a key role in optimizing treatment options and conducting clinical trials on targeted drugs [ 10 ]. (biomedcentral.com)
  • Type 1 diabetes (T1D) results from autoimmune destruction of insulin-producing pancreatic beta cells, novel biomarkers are greatly in need to indicate this destruction process. (uncg.edu)
Epithelial7
  • We isolated four categories of cells from mammary epithelium of female calves: bromodeoxyuridine label retaining epithelial cells (LREC) from basal (LRECb) and embedded layers (LRECe), and epithelial control cells from basal and embedded layers. (frontiersin.org)
  • whereas LREC in suprabasal epithelial layers are enriched for more committed progenitor cells, expressing some genes that are associated with stem cell attributes along with those indicative of cell differentiation. (frontiersin.org)
  • This dictates cycles of mammary growth, differentiation, lactation, and regression, during which mammary stem cells (MaSC) provide for the lineages of luminal and basal (myoepithelial) epithelial cells in the ducts and alveoli. (frontiersin.org)
  • During rapid mammary growth in the mouse, label retaining epithelial cells (LREC) appear to retain label by asymmetric distribution of DNA strands, as evidenced by a rapid proliferation index of the LREC ( Smith, 2005 ). (frontiersin.org)
  • K17 was not detected in the epithelial cells of benign glandular lesions, but groups of cuboidal cells, residing beneath the epithelial layer of benign glands, were frequently positive for K17, especially in cases of microglandular hyperplasia. (medscape.com)
  • For example, the epithelial−mesenchymal transition phenotype presents more cells with a lower degree of differentiation and greater cell dissociation in the ITF than in other tumor areas 14 , 15 . (nature.com)
  • Since epithelial cell signaling during NEC has been an important factor in deciphering the pathogenesis of the disease [ 11 , 12 ], we focused this study on the epithelial cell methylome signature. (biomedcentral.com)
Proteins7
  • The high diagnostic potential of peptides and proteins from cells, tissues, and biological fluids is unquestionable. (genengnews.com)
  • Analysis of immunophenotypically defined cell populations markedly increased specificity of liquid chromatography-tandem mass spectroscopy (LC-MS/MS). Over 375 differentially expressed proteins were analyzed by Ingenuity Pathway Analysis (Ingenuity Systems). (genengnews.com)
  • RESULTS: Our proteome analysis identified 68 significantly, at least two-fold upregulated proteins in Abi resistant cells. (bvsalud.org)
  • DNA mutations lead to abnormal proteins or missing functional proteins, which can cause cells to multiply uncontrollably and become cancerous. (leica-microsystems.com)
  • A piRNA-like small RNA interacts with and modulates p-ERM proteins in human somatic cells. (omicsdi.org)
  • piRNA-like-163 (piR-L-163), the top downregulated piRNA-L in NSCLC cells, binds directly to phosphorylated ERM proteins (p-ERM), which is dependent on the central part of UUNNUUUNNUU motif in piR-L-163 and the RRRKPDT element in ERM. (omicsdi.org)
  • Launch Multicellular microorganisms are comprised of an array of cells that are grouped into different kinds based on phenotypic traits such as for example area morphology lineage origins and proteins or RNA biomarker profile. (ecologicalsgardens.com)
Mass spectrometry1
20201
  • July 2, 2020 Repeated measurements of the biomarker FIB-4 in the blood every few years can predict the risk of developing severe liver disease, according to a new study. (sciencedaily.com)
MRNA11
  • Single-cell transcriptomics examines the gene expression level of individual cells in a given population by simultaneously measuring the RNA concentration (conventionally only messenger RNA (mRNA)) of hundreds to thousands of genes. (wikipedia.org)
  • Semi-quantitative evaluation of mRNA amounts was performed by real-time PCR using SYBR Green and gene appearance profile of c-kit-transfected and control PCa cells was analyzed using a gene-focused array (find Supporting Information Components and Strategies). (acancerjourney.info)
  • A piRNA utilizes HILI and HIWI2 mediated pathway to down-regulate ferritin heavy chain 1 mRNA in human somatic cells. (omicsdi.org)
  • Since the current piRNA-mediated knockdowns of target mRNA are mostly reported in germ line cells, piRNA-mediated post-transcriptional gene regulation in somatic cells is rather unique in its application and mechanistically uses an alternative pathway to siRNA and miRNA. (omicsdi.org)
  • The TIVA technique provides the initial noninvasive strategy for recording mRNA from one cells within their organic microenvironment. (ecologicalsgardens.com)
  • Nevertheless RNA-seq of one cells is bound by the specialized challenges connected with isolating mRNA from one cells. (ecologicalsgardens.com)
  • This is also true for cells in complicated tissues like the brain where in fact the mobile connective intricacy of intermingling neurons and glia makes one cell mRNA isolation difficult. (ecologicalsgardens.com)
  • Therefore an mRNA catch methodology that's appropriate for live intact tissues and that allows Gossypol mRNA catch with precise spatial quality would give a useful device to explore the transcriptomes of one cells in the framework of their organic microenvironment with small bias from RNA contaminants or experimentally-related damage. (ecologicalsgardens.com)
  • Here we explain a novel technique for isolating mRNA in morphologically complicated tissue and with the spatial quality of an individual cell utilizing a photoactivatable mRNA catch molecule known as the TIVA-tag. (ecologicalsgardens.com)
  • We demonstrate the tool from the TIVA-tag in both cell lifestyle and brain Gossypol tissues for catch of one cell mRNA for following RNA-seq transcriptome evaluation. (ecologicalsgardens.com)
  • Outcomes The TIVA-tag catches mobile mRNA upon photoactivation To execute transcriptome evaluation of individually chosen cells Gossypol in unchanged tissue we built a multifunctional photoactivatable mRNA catch molecule that people contact the TIVA-tag. (ecologicalsgardens.com)
Populations4
  • Single-cell transcriptomics makes it possible to unravel heterogeneous cell populations, reconstruct cellular developmental pathways, and model transcriptional dynamics - all previously masked in bulk RNA sequencing. (wikipedia.org)
  • Higher throughput and speed allow researchers to frequently characterize the expression profiles of populations of thousands of cells. (wikipedia.org)
  • The data from bulk assays has led to identifying genes differentially expressed in distinct cell populations, and biomarker discovery. (wikipedia.org)
  • Advantages of ICM over flow cytometry include low set up cost, smaller number of nuclei required and greater sensitivity for non-diploid cell populations. (nature.com)
Tissues9
  • These studies are limited as they provide measurements for whole tissues and, as a result, show an average expression profile for all the constituent cells. (wikipedia.org)
  • Establish the primary cultured cell lines from the HR cell-xenografted tumor tissues during different stages of Herceptin® treatment. (tmu.edu.tw)
  • In this study, we used the deep sequencing of small RNA Transcriptome to explore the piRNA expression in six clear cell renal carcinoma (ccRCC) tissues and matched adjacent normal tissues and found that six piRNAs were upregulated and sixteen were downregulated in ccRCC tissues. (omicsdi.org)
  • Quantitative real-time PCR (qRT-PCR) was used to confirm piR-31115 expression in other ccRCC tissues (n = 40) and ccRCC cell lines. (omicsdi.org)
  • LCM was able to accurately capture 50-200 cells from HCC and control tissues. (geneticsmr.org)
  • catch from one cells in live tissues. (ecologicalsgardens.com)
  • However the research of one mammalian cells in tissues can help in deciphering the resources of one cell variability and specifically the way the microenvironment establishes variability in cells of apparently the same type. (ecologicalsgardens.com)
  • Notably the tissues microenvironment made by specific neighboring cells could be significantly diverse which is anticipated that with extracellular heterogeneity comes gene appearance heterogeneity. (ecologicalsgardens.com)
  • Therefore equipment that check out the transcriptome from one cells in tissues would give a unique chance of evaluating mammalian cell heterogeneity and its own natural importance. (ecologicalsgardens.com)
Serum3
  • Our next step is to search for these biomarkers in serum," asserts Dr. Lubman. (genengnews.com)
  • Imaging technologies provide good tools for this purpose, but can still benefit from additional information such as blood-based serum biomarkers that may pick up the small changes of cellular function. (genengnews.com)
  • The aim of this study was to identify predictive serum biomarkers for Abi treatment. (bvsalud.org)
Mutations1
  • In conclusion, BRAF mutations in histiocytic proliferations are restricted to lesions of the Langerhans-cell type. (oncotarget.com)
Pathways6
  • Enriched expression of genes in LRECb was associated with stem cell attributes and identified WNT, TGF-β, and MAPK pathways of self renewal and proliferation. (frontiersin.org)
  • 3. Study the action of Herceptin-induced HNMT overexpression and the mechanisms that cause HR. Aim 3: Establish HNMT inhibition (siRNA) and overexpression cell lines to clarify the HR-related signaling pathways Year-2 proposal: Study the mechanisms of HNMT-mediated gene regulation in the HR cells Aim 1: Establish the HR cells for transcriptome sequencing analysis 1. (tmu.edu.tw)
  • We will also shortly discuss how leveraging single cell multi-omic approaches can be used to accelerate cellular biomarker discovery during clinical trials to predict response to therapy, follow responsive cell types, and define novel druggable target pathways. (frontiersin.org)
  • Evaluation of activity of multiple areas of cell signalling pathways and procedures (e.g. (academicediting.org)
  • Alterations in gene sequence or expression can occur in the cell-signalling and regulatory pathways involved in cell-cycle control, apoptosis, proteosome regulation and angiogenesis. (bmj.com)
  • Over-expression of the most down-regulated piRNA in GBM tissue, piR-8041, was found to reduce glioma cell line proliferation, induce cell cycle arrest and apoptosis, and inhibit cell survival pathways. (omicsdi.org)
Efficacy1
  • This review considers current evidence linking biomarker profile to efficacy of EGFR-targeted therapy in NSCLC, and clinical implications of recent findings. (ersjournals.com)
Isolate2
  • Low throughput techniques are able to isolate hundreds of cells, are slow, and enable selection. (wikipedia.org)
  • We developed a qPCR-based platform that could be used to isolate individual cells from native tissue and obtain highly comparable gene expression analyses. (leica-microsystems.com)
Diagnostic1
  • Although p16 INK4a has been well established as sensitive and accurate diagnostic biomarker for endocervical adenocarcinoma in situ and invasive adenocarcinoma in histologic sections and in cervical cytology specimens, clinical and diagnostic features alone have limited power to predict the survival of patients with endocervical adenocarcinoma. (medscape.com)
Identification1
  • The resulting instrument platform is expected to have broad applications in structural identification of intact lipids and will aid the unambiguous identification of lipid biomarkers of diseases, and improve our understanding of the functional roles of diverse lipids in the disease process. (uncg.edu)
Apoptosis1
  • Flow cytometry was used to detect cell cycle and apoptosis. (besjournal.com)
Renal cell carc2
  • Therefore, we aimed to compare the differential expressions of PD-1, PD-L1 and PD-L2 between the primary and metastatic sites of renal cell carcinoma (RCC). (biomedcentral.com)
  • Renal cell carcinoma (RCC) is originated from the epithelium of renal tubules. (biomedcentral.com)
Genome6
  • Here are the latest publications comparing PicoPLEX technology to other single-cell whole-genome amplification technologies, and using PicoPLEX technology to uncover insights from CTCs, single cells from FFPE tumor samples, and samples relevant to preimplantation genetic testing. (takarabio.com)
  • This paper compared the performance of three commercially available whole genome amplification (WGA) technologies: the PicoPLEX WGA Kit , the REPLI-g Single Cell Kit, and the Ampli1 WGA Kit. (takarabio.com)
  • This paper compared the performance of the PicoPLEX DNA-seq Kit , the DOPlify WGA Kit, the REPLI-g Single Cell Kit, and the Ampli1 WGA Kit for aneuploidy screening and copy number analysis using shallow whole genome sequencing, starting from one, three, or five cells isolated from the Loucy cell line. (takarabio.com)
  • Cells were whole-genome amplified, followed by PCR-free library preparation and Illumina sequencing. (takarabio.com)
  • This paper compared the performance of copy number variation (CNV) detection of four commercially available whole genome amplification (WGA) kits: the PicoPLEX WGA Kit , the GenomePlex Single Cell Whole Genome Amplification Kit, the MALBAC Single Cell Whole Genome Amplification Kit, and the REPLI-g Single Cell Kit. (takarabio.com)
  • Utilizing single cell genome, methylome, chromatin accessibility, while RNA or protein from the same cell can be used to profile the transcriptome, and proteome, different single cell omics profiles alone or in combination can serve as building blocks to construct a multi-omics profile for the same cell. (frontiersin.org)
Proliferation6
  • During periods of low mammary proliferation, quiescence of the stem cell population may account for retention of label. (frontiersin.org)
  • Cell Proliferation Migration Invasion and Anoikis Cell viability and proliferation of PCa cells stably transfected using the vector expressing c-kit or the unfilled vector were examined utilizing the WST-1 assay within the existence or lack of 100 ng/ml SCF (PeproTech). (acancerjourney.info)
  • Cell proliferation was measured by CCK8 and EdU assay. (besjournal.com)
  • Interestingly, ULK2 inhibited CRC cell proliferation in a p53-dependent manner. (besjournal.com)
  • Furthermore, exosome-derived miR-224-5p partially reversed the proliferation regulation of ULK2 on CRC cells. (besjournal.com)
  • Conclusion Our findings demonstrate that exosome-transmitted miR-224-5p promotes p53-dependent cell proliferation by targeting ULK2 in CRC, which may offer promising targets for CRC prevention and therapy. (besjournal.com)
Protein3
  • Isolation of multiple types of molecules (DNA, RNA, or protein) from a single cell simultaneously, stands at the beginning of each approach and having standardized and validated protocols for single cell solutions is surely the foundation of all the herein described approaches. (frontiersin.org)
  • Consequently, new proteomic technologies are being developed to monitor and identify protein function and interactions responsible for the total activities of the cell. (usuhs.edu)
  • The protein expressions of p53 and unc-51 like kinase 2 (ULK2) in CRC cells were detected by western blot. (besjournal.com)
Circulating1
  • It will also be discussed how circulating biomarkers are starting to be used in routine clinical practice to guide standard-of-care treatment decisions and selection of patients to early phase clinical trials. (uib.no)
Clinical4
  • Aim-2: Establish the HNMT-HR cell-xenografted tumor model for drug screening Herceptin-induced the HNMT/HER2 complex can be used as a predictor for clinical applications. (tmu.edu.tw)
  • MAPK PI3K Wnt NFKB apoptotic cell bicycling tumour microenvironment immune system regulation) could be required to anticipate the clinical aftereffect of following BRAF inhibition. (academicediting.org)
  • In clinical practice, the detection of biomarkers is mostly based on primary tumors for its convenience in acquisition. (biomedcentral.com)
  • l in T cells, 5HN generates superoxide and H2O2 to activate NF-B in a dose-dependent manner, and as a result is capable to reactivate HIV, notably without having causing widespread T cell activation (which would indicate that the molecule is as well toxic for clinical use) (Yang et al. (nicotinic-receptor.com)
20211
  • Additional analysis into molecules for instance 5HN that may exploit this association may perhaps prove useful in discovering new strategies to reactivate HIV without having the induction of international T cell activation.S. Buckley et al.Brain, Behavior, Immunity - Overall health 13 (2021) 100235 Ayala, A., Munoz, M.F., Arguelles, S., 2014. (nicotinic-receptor.com)
Fluorescence-activated cell2
  • Common techniques include: Fluorescence activated cell sorting (FACS) Microfluidic devices Combining FACS with scRNA-seq has produced optimized protocols such as SORT-seq. (wikipedia.org)
  • Meanwhile, Len Herzenberg, who was interested in a machine that could sort living cells based on their fluorescence, applied the design of the Fulwyler particle separator to build the first fluorescence activated cell sorting (FACS) instrument at Stanford University in the late 1960s (see the video Inventing the Cell Sorter, Herzenberg Lab, https://www.youtube.com/watch?v=Ro8P3w9BPhg ). (frontiersin.org)
Detection1
  • Analysis of PicoPLEX WGA products provided a correct diagnosis for 16/16 cells, with detection of 16/16 individual aneuploidies. (takarabio.com)
Differentiation1
  • Gotic I, Schmidt WM, Biadasiewicz K, Leschnik M, Spilka R, Braun J, Stewart CL, Foisner R (2010): Loss of LAP2 alpha delays satellite cell differentiation and affects postnatal fiber-type determination. (fh-krems.ac.at)
Metabolomic1
  • In this review we will discuss how combinations and fusions of different -omic workflows on a single cell level can be used to examine cellular phenotypes, immune effector functions, and even dynamic changes, such as metabolomic state of different cells in a sample or even in a defined tissue location. (frontiersin.org)
Metastasis1
  • However despite such obvious evidence suggesting the induction of c-kit manifestation is triggered by the osseous microenvironment Rotigotine as a result of an Rotigotine adaptation of the PCa bone colonizing cells the contribution of c-kit manifestation and activation in PCa cells to bone metastasis has not been confirmed up to date. (acancerjourney.info)
Gene8
  • Recent advances in biotechnology allow the measurement of gene expression in hundreds to thousands of individual cells simultaneously. (wikipedia.org)
  • Year-3 proposal: Animal studies on the mechanisms of HNMT-mediated gene regulation in the HR cells Aim 1: Establish the HNMT-HR cell-xenografted tumor model 1. (tmu.edu.tw)
  • To discard nonspecific results on gene appearance cells had been transiently transfected using the pcDNA3 unfilled vector (Invitrogen). (acancerjourney.info)
  • Before using transduced PCa cells gene induction was verified using cocultures of Rotigotine Computer3 and bone tissue marrow (BM) cells inserted in Matrigel (BD Biosciences) which imitate c-kit induction seen in intraosseous PCa tumors or metastases6. (acancerjourney.info)
  • However, piRNA-mediated post-transcriptional gene regulation in human somatic cells is not well understood. (omicsdi.org)
  • Microarrays may be used to assay gene expression within a single sample or to compare gene expression in 2 different cell types or tissue samples, such as in healthy versus diseased tissue. (medscape.com)
  • A lot of the understanding of gene appearance variability continues to be extracted from research using one cell microorganisms such as bacterias or cells normally occurring in suspension system4-7. (ecologicalsgardens.com)
  • It really is unknown if the procedures that govern gene appearance variability among unicellular microorganisms could be extrapolated towards the cells of multicellular microorganisms. (ecologicalsgardens.com)
NSCLC1
  • Distinctive piRNA/piRNA-L expression patterns are observed between HBE and NSCLC cells. (omicsdi.org)
Biology5
  • Insights into the biology of stem cells will be gained by confirmation and characterization of candidate MaSC markers identified in this study. (frontiersin.org)
  • Since the early days of cell biology scientists have been using optical instruments to identify cell types in homeostatic conditions and diseases. (frontiersin.org)
  • Specifically it will be discussed how these assays may be applied in the setting of early phase drug development as predictive, pharmacodynamic and resistance biomarkers and as a research tool to understand more about the biology of disease to guide future drug targets and combinations. (uib.no)
  • Henriette stages stories that takes place inside the body, more specifically within the individual cell, and introduce that teeny tiny biology not visible even in the microscope. (uib.no)
  • Having also studied acting she is well suited to tell the story of the wondrous biology of cells. (uib.no)
HER21
  • Observation of HNMT/HER2 complex formation in live cell models by Split Luciferase, FRET, and FLIM analysis to prove its association with HR. 2. (tmu.edu.tw)
Tumour1
  • Prior research of heterogeneity centered on the BRAF genotype evaluating various mutation examining techniques entirely tumours microdissection of tumours or single-cell analyses (Lin et al 2009 Wilmott et al 2012 Yancovitz et al 2012 The last mentioned techniques are frustrating costly and can't be easily performed on a lot of tumour examples as was performed in our study. (academicediting.org)
Overexpression1
  • Seeböck R, Laimer K, Bachmann F, Spizzo G, Vogetseder A, Wieser M, Müller H, Haybaeck J, Obrist P (2012): Overexpression of eIF3a in Squamous Cell Carcinoma of the Oral Cavity and Its Putative Relation to Chemotherapy Response. (fh-krems.ac.at)
Samples1
  • With the wide introduction of flow cytometry in the early 70-ies markers and subsequent cell types have evolved but it was only in the last decade that the introduction of single cell transcriptome sequencing, high dimensional cytometry and imaging cytometry started revolutionizing the way we interrogate biological samples. (frontiersin.org)
Genes1
  • Furthermore we demonstrate the contribution of bone-induced c-kit manifestation by PCa cells to intraosseous tumor formation and confirmed high and low expressions of and genes respectively in intraosseous PCa tumors inside a mouse model and bone metastases from PCa individuals. (acancerjourney.info)
Experimental1
  • To do this, experimental and control arms can be stratified by biomarker status and an interaction test performed. (ersjournals.com)
Specific4
  • Previous work in our laboratory identified keratin 17 (K17) as a specific and sensitive biomarker for high-grade squamous intraepithelial lesions and cervical squamous cell carcinoma (SCC). (medscape.com)
  • The HIV/AIDS pandemic in the 1980s then gave a dramatic impulse to the technology of counting specific cells, since it became clear that the quantification of peripheral blood CD4+ T cells was crucial to follow the course of the infection, and eventually for monitoring response to therapy ( 2 ). (frontiersin.org)
  • however, these biomarkers are not specific for amyloid. (medscape.com)
  • Using the TIVA-tag in conjunction with RNA-seq to investigate transcriptome variance among one dispersed cells and citizen mouse and individual neurons we present which the tissue microenvironment forms the transcriptomic landscaping of specific cells. (ecologicalsgardens.com)
Proteome1
  • Single cell proteome approaches already have changed how we explore cellular mechanism in disease and during therapy. (frontiersin.org)
RNAs1
  • The Single-cell RNA-seq technique converts a population of RNAs to a library of cDNA fragments. (wikipedia.org)
Tumors3
  • The presence of neoplastic islands, classified as large or small according to the number of cells in the ITF, has been described as the most aggressive pattern compared to tumors with a more uniform growth pattern, as tumor invasion occurs in a more widespread manner as cellular islands or single cells 4 . (nature.com)
  • Loss of BRCA2 function stimulates prostate malignancy (PCa) cell invasion and is associated with more aggressive and metastatic tumors in PCa individuals. (acancerjourney.info)
  • manifestation of c-kit using two different intraosseous tumor models and three additional human being PCa cell lines which proved to be c-kit-negative when cultivated or as tumors in non-osseous cells. (acancerjourney.info)
Isolation1
  • A key challenge in CTC isolation and characterization has been their extreme rarity in blood and their cell-to-cell heterogeneity. (ubc.ca)
Cellular1
  • In synergy with constantly adapting new single-cell analysis approaches and subsequent accumulating big data collections from these platforms, whole atlases of cell types and cellular and sub-cellular interaction networks are created. (frontiersin.org)
Discovery1
  • Biomarker discovery requires both reduction of sample complexity and retention of coverage," he explains. (genengnews.com)