Biology targets biological assay. Medical search. Frequent questions

Founded in 1998 and headquartered in Cambridge, UK, Abcam offers the scientific community highly validated antibodies, reagents, biomarkers and assays to address targets in biological pathways that are critical for advancing drug discovery, life sciences research, and diagnostics. (onenucleus.com)
GeneGo's flagship product, MetaCore 4.6 TM , assists pharmaceutical scientists in the areas of target selection and validation, identification of biomarkers for disease states and toxicology. (cff.org)
With a sensitive enough detection method, they contend, skin's biomarkers could provide enough biological information that you wouldn't need trained medical staff to run these painless tests. (theceliacscene.com)

Moreover, homogenates of footpads do not contain activin-like activity in the neuronal assay in vitro. (caltech.edu)
With the recent advances in human-cell cultivation techniques, allowing in vivo -like in vitro long-term functionality, there is a shift in focus towards the mechanistic details of the adverse effects "over time" aimed at a better understanding of the dynamics of biological processes. (brill.com)

The array format provides this standard in five predispensed, sequential 10-fold dilutions mixed with a PCR primer assay in triplicate, ensuring that your sample library will fall within the detection range of the array (see figure Principle of the QIAseq Library Quant System The serial dilutions of the DNA standard (5 sequential 10-fold dilutions) generate a standard curve. The sample library should fall within the standard curve. "> Principle of QIAseq Library Quant System ). (qiagen.com)
In response to this need, three types of diagnostic tests for SARS-CoV were quickly developed: tissue culture isolation, antibody detection, and reverse transcription-polymerase chain reaction (RT-PCR) assays. (cdc.gov)
This may often involve the simultaneous detection of multiple targets in a single reaction well, using different pairs of primers for each target, with various flurophores or channels of colors available for use as probes. (labmanager.com)
Bio-Rad's CFX Opus Real-Time PCR Systems utilize solid-state light-emitting diodes (LEDs) for fluorescence excitation and photodiodes for detection. (labmanager.com)
The principle of this approach is based on the combination of MB immunocapture-based enzyme activity assay and competitive immunoassay of the total amount of enzyme for simultaneous detection of enzyme inhibition and phosphorylation in biological fluids. (cdc.gov)
It's below the assay detection limit, but they recur later. (medscape.com)

Cell-based assays are becoming increasingly popular for small molecule discovery in high-throughput screening. (genengnews.com)
As cell-based assays are used more commonly in robotic high-throughput compound screening, cells themselves have become critical reagents. (genengnews.com)
Topics covered include various high-throughput genomics technologies (genome sequencing, DNA microarrays, proteomics), quantitative modeling of transcriptional and post-transcriptional regulatory networks, synthetic biology, and the world of RNA structure, dynamics, and function. (columbia.edu)
Topics covered will include high-throughput assay development, screening, chemical library creation, high-throughput chemistry, affinity purification of target proteins and target validation, protein microarrays, molecular evolution, protein engineering and synthetic biology. (columbia.edu)
Available in three different block configurations including the CFX Opus 96 Real-Time PCR System (for general qPCR applications), CFX Opus 384 Real-Time PCR System (for high throughput labs), and CFX Opus Deepwell Real-Time PCR System (for larger volumes up to 125 μl), Bio-Rad qPCR instruments are distinguished by the use of a unique optical shuttle system. (labmanager.com)
The MLP anticipates funding 6-10 centers, (i.e. 2-4 comprehensive, high throughput centers, 2-3 high value target, lower throughput specialized screening centers, and 2-3 specialized chemistry centers). (nih.gov)
Despite this, drug discovery for AQPs has made little progress due to a lack of reproducible high-throughput assays and difficulties with the druggability of AQP proteins. (lu.se)

Though many traditional sensing technologies for biomarker analysis and fundamental biological studies (i.e. enzyme-linked immunosorbent assays, real-time polymerase chain reaction, etc.) rely on single-parameter measurements, it has become increasingly clear that the inherent complexity of many human illnesses and pathways necessitates quantitative and multiparameter analysis of biological samples. (illinois.edu)
Providing the research and clinical communities with tools and scientific support, the Company offers highly validated antibodies, assays, and other research tools to address important targets in critical biological pathways. (onenucleus.com)
Familiarity with signal transduction pathways misregulated in cancer, regulation of cell division, transcription factor biology, or DNA damage response pathways. (lookforzebras.com)
The lab's efforts are mainly focused on the rational design, synthesis, and biological evaluation of drug-like small molecules that can modulate cellular signaling pathways with an emphasis on protein-protein interactions (PPIs) and target specificity. (usf.edu)
The second is the application of new tools to designing selective PPI inhibitors for clinically highly relevant signaling pathways and characterizing the biological activities of new inhibitors using biochemical and cell-based assays. (usf.edu)
The original computational MetaDiscovery TM platform allows an integration and expert analysis of different kinds of experimental data (mRNA expression, proteomics, metabolomics, microRNA assays and other phenotypic data) and relevant bioactive chemistry (metabolites, drugs, other xenobiotics) within the framework of curated biological pathways and networks. (cff.org)
This work will elucidate how RG4-mediated functions contribute to cellular mRNA homeostasis, and will identify physiologically significant RG4-binding partners, which in turn may reveal molecular targets and pathways with therapeutic potential. (grantome.com)

As an alternative or complementary method to assess the NEO cassette copy number, we set up an assay based on quantitative real-time PCR (qPCR). (biomedcentral.com)
Currently used analytical methods are deficient in that they often provide either highly quantitative data for a single biomarker or qualitative data for many targets, but methods that simultaneously provide highly quantitative analysis of many targets have yet to be adequately developed. (illinois.edu)
Fields such as medical diagnostics and cellular biology would benefit greatly from a technology that enables rapid, quantitative and reproducible assays for many targets within a single sample. (illinois.edu)
Redistribution assays are quantitative assays that can be run in standard microtiter plates. (genengnews.com)
As the life sciences become an increasingly quantitative discipline, Bio-IT World provides topical news coverage and analysis of cutting-edge technologies to handle the data deluge in petascale computing and the tools to deliver individualized medicine. (bio-itworld.com)
Prior to being appointed as head of DPTM, George was vice president of Quantitative Biosciences (QB) responsible for pre-clinical pharmacological assay development across disease areas and he led the implementation of new techniques and technologies to enable objective interrogation of biological systems to improve the prioritization of early-stage pipeline candidates. (phrmafoundation.org)

QIAseq DNA QuantiMIZE System utilizes 2 qPCR assays that interrogate 20 genomic loci to determine the amounts of amplifiable DNA in biological samples. (qiagen.com)
QuantiMIZE Kits use the C T values of Assay 100 and Assay 200 for both the Control samples and the Case samples to quantify and qualify DNA (see Principle of the QIAseq DNA QuantiMIZE System. The QIAseq DNA QuantiMIZE System utilizes 2 qPCR assays that interrogate 20 genomic loci to determine the amounts of amplifiable DNA in biological samples. "> Principle of the GeneRead DNA QuantiMIZE System ). (qiagen.com)
Genomic DNA extracted from biological samples is mixed with the supplied mastermix. (qiagen.com)
Use the PrimePCR DNA contamination control assay to determine if genomic DNA (gDNA) is present in a sample at a level that may affect PCR results. (bio-rad.com)
Accordingly, our research program is focused on genomic tumor biology and the subsequent functional elucidation of biological mechanisms. (lu.se)
To do this, we will perform genome-wide siRNA screens on MITF-high and -low melanoma cells in combination with genomic analysis and suitable functional assays. (lu.se)

Our LanthaScreen™ Cellular Assays detect posttranslational modifications, such as ubiquitination of specific target proteins that take place within the native cellular environment. (thermofisher.com)
Loss-of-function and gain-of-function approaches were applied in cellular assays, including cell viability, migration, invasion, and apoptosis. (jcancer.org)

Despite this key shortcoming, biochemical assays are often preferred over cell-based assays due to perceived differences in complexity, time, and cost. (thermofisher.com)
This laboratory position supports discovery research efforts for the Oncology portfolio with target validation experiments, cell-based and biochemical assays, and mechanism of action studies. (lookforzebras.com)
Familiarity with biochemical assays, determination of Km, assessing small molecules for enzyme inhibition. (lookforzebras.com)

The QIAseq DNA QuantiMIZE System is a qPCR-based approach to determine quantity and quality of DNA that is amenable to amplification by targeted enrichment prior to NGS. (qiagen.com)
The Bio-Rad™ approach uses a novel optical design with a fundamentally different approach toward qPCR. (labmanager.com)

www.cmt-inc.net), makes it possible to use frozen assay-ready cells for screening as if they were ordinary assay reagents like enzymes or membrane preparations. (genengnews.com)
Human IgG antibody Laboratories manufactures the malachite green phosphate assay protocol reagents distributed by Genprice. (urokinases.com)
The assay sensitivity is what we do in the lab with limiting dilution of reagents, and we say, what's the least amount of molecules we can detect? (medscape.com)

During a recent work, where a knockin SCA28 mouse was prepared, we developed two assays based on Real-Time PCR using both SYBR Green and specific minor groove binder (MGB) probes to evaluate the copies of NEO using the comparative delta-delta Ct method versus the Rpp30 reference gene. (biomedcentral.com)
We compared the results from Southern blot, routinely used to quantify NEO copies, with the two Real-Time PCR assays. (biomedcentral.com)
Bio-Rad collaborated with Biogazelle, leaders in real-time PCR research, to design and experimentally validate PCR primers for gene expression assays across the human and mouse transcriptomes. (bio-rad.com)
The resulting scores were used to select the assays included in the corresponding real-time PCR pathway panel. (bio-rad.com)
Control assays and synthetic DNA templates were designed to facilitate the assessment of the key experimental factors impacting your real-time PCR results. (bio-rad.com)

We use a broad repertoire of biochemistry, cell biology, live cell imaging and animal models to study the impact of mitochondria shape, number and subcellular distribution in metastatic dissemination. (cuanschutz.edu)
In this course, we will cover subject matter in biochemistry, organic chemistry and structural biology. (columbia.edu)
The course is intended to provide a foundation of structural biology, bio-organic chemistry and biochemistry and to expose students to some emerging areas of research. (columbia.edu)
This course contains mainly molecular biology, biochemistry & genetic content, is based on reading research articles and requires writing a research proposal. (columbia.edu)
Emerging proteomic technology, protein microarrays, is gaining rapid popularity in molecular biology and biochemistry. (headlinesoftoday.com)
He earned a B.A. in Biochemistry from Rutgers College, a Ph.D. in Chemistry from the University of Virginia and conducted postdoctoral research at Harvard Medical School in the Department of Biological Chemistry and Molecular Pharmacology. (phrmafoundation.org)

The track will focus on emerging targets and mechanisms including protein degradation, non-coding RNAs and stabilising PPIs. (slas.org)
L ike protein phosphorylation by kinases, protein ubiquitylation regulates many aspects of cell function and provides a wealth of drug target opportunities across many therapeutic areas including cancer, cardiovascular, metabolism, inflammation, neurodegeneration and infectious diseases. (ddw-online.com)
However, over the same period protein kinases have rapidly become one of the most significant classes of drug targets for the pharmaceutical industry, with the global market for kinase therapies being about US$15 billion per annum in 2010 and this value is predicted to double by 2020 (3). (ddw-online.com)
In general, a DEL has been considered as a massive binding assay to identify physical binders for individual protein targets. (nature.com)
Oligonucleotide which displays specific binding to a protein or other target, often selected by an iterative cycle of affinity- based enrichment. (genomicglossaries.com)
Though this technology can be used to detect a variety of molecules, this dissertation establishes the utility of microring resonator chips for multiparameter analysis of several challenging protein targets in cell cultures, human blood sera, and other clinical samples such as cerebrospinal fluid. (illinois.edu)
In a typical experiment, microfluidics are used to spatially control chip functionalization by directing capture antibodies against a variety of protein targets to groups of microring sensors. (illinois.edu)
This dissertation describes the fabrication, characterization, development, and application of silicon photonic microring resonator technology to multiplexed protein measurements in a variety of biological systems. (illinois.edu)
Redistribution assay technology relies on the imaging and quantification of intracellular protein translocation. (genengnews.com)
G protein-coupled receptors (GPCRs) account for some 50 percent of all current drug targets, says Arne Heydorn, scientist at BioImage, whereas marketed drugs only target about 30 percent of the GPCRs in the genome. (genengnews.com)
Four critical lab components include protein structure-based drug design, multiple-step synthesis, and molecular and cellular biology. (usf.edu)
We report data on 166,174 proteotypic peptides providing multiple, independent assays to quantify any human protein and numerous spliced variants, non-synonymous mutations, and post-translational modifications. (nih.gov)

Xenopus is increasingly being used as imaging test-bed to investigate the roles of cytoskeleton and intracellular trafficking in cell biological and morphogenetic contexts. (cshl.edu)
Cell-based assays offer valuable insights into intracellular molecular targets, providing the advantage of biological context. (headlinesoftoday.com)

Within the broad scope of systems biology, my lab focuses on 3 research areas: 1) Network inference for identifying drug targets, 2) Predicting drug sensitivity from -omics datasets, and 3) Modeling temporal effects of drug combinations. (cuanschutz.edu)
The assay will be functionally equivalent, yet clinically superior, to whole metagenome sequencing, but at 1/10th the cost, 1/3rd the time, and several times the sensitivity. (labroots.com)
First of all, let's not confuse the assay sensitivity with its clinical sensitivity. (medscape.com)

Sessions will cover the process of translating biological insight into tractable assays, efficient tool compounds and biologicals, and methods for generating attractive starting points for drug discovery programmes. (slas.org)
Target-focused compound libraries have been a key enabling component of the tool kit opening up kinase drug discovery, consisting of collections of compounds designed to interact with a family of related kinase targets (4). (ddw-online.com)
Such libraries are used for screening against therapeutic targets in order to find hit compounds that may be further developed into drugs and/or used as research tools to better understand the underlying biology and its relevance to pharmacological intervention. (ddw-online.com)
Majority of the synthesized compounds get filtered out in this step, hence its extremely important to set up the assays in such away that the outcome provide true reliable measure of effect of each compound. (brighttalk.com)
We developed Redistribution cell-based assays for use in both primary screening and lead optimization of compounds targeting GPCRs. (genengnews.com)
The ToGA trial must be characterized as a landmark in the treatment of gastric cancer and it has paved the way for a number of new HER2 targeted compounds such as pertuzumab, ado-trastuzumab emtansine, lapatinib, afatinib, and dacomitinib, which are currently undergoing phase II and III clinical testing. (wjgnet.com)
The second product, MetaDrug 4.6TM is designed for prediction of human metabolism, toxicity and biological effects for novel small molecules compounds. (cff.org)
The goal of the MLP is to screen compounds in the Small Molecule Repository in target-based and phenotypic assays to identify and subsequently optimize small molecule s as research probes. (nih.gov)

The system has been developed for discovery, translational, and clinical researchers who need a cost-effective way to determine the optimum conditions for targeted enrichment of DNA isolated from biological samples, including FFPE. (qiagen.com)
The real-time RT-PCR assay was more sensitive than a conventional RT-PCR assay or culture isolation and proved suitable to detect SARS-CoV in clinical specimens. (cdc.gov)
This position utilizes a wide range of cell and molecular biology skills and works in close collaboration with other scientists as an integral member of the drug discovery team to advance programs from conception to clinical development. (lookforzebras.com)
Fluorescent antibodies find essential roles in clinical therapy and the investigation of immunology, cell biology, and neuroscience. (headlinesoftoday.com)
The Centre for Brain Development and Repair has come up inside the campus of Institute of Stem Cell Biology and Regenerative Medicine to study genetic, neural and behavioural components of autism to find a clinical answer to the disorder that afflicts lakhs of children. (deccanherald.com)
Since the identification of somatic mutations in the BRAF oncogene, targeted therapies directed toward mutated tumors have emerged and demonstrated dramatic clinical effect. (lu.se)
Ultimately, our results will improve our understanding of the complexity of melanoma biology as well as having an impact in the clinical setting as such information can be used to develop prognostic and predictive molecular signatures. (lu.se)

Although the widespread use of target-focused libraries has led to the rapid expansion of novel chemistry as research tools in drug discovery to exploit many classes of drug target, the ubiquitin system still remains a largely untapped medicinal chemistry opportunity. (ddw-online.com)
The course is intended for advanced undergraduates as well as beginning graduate students in Biology, Chemistry, Physics, Engineering, and Computer Science. (columbia.edu)
The course will be of interest to students at the interface between chemistry and biology, and students interested in medicine, academic chemical biology and drug discovery efforts. (columbia.edu)
This four and a half-credit course will present an introduction to the Biophysical Chemistry and Structural Biology of proteins and nucleic acids. (columbia.edu)
I study chemistry in college along with biology, physics, and maths, and I have a general interest in all of them. (stackexchange.com)
Dr. Ji's research is Medicinal Chemistry and Chemical Biology. (usf.edu)
Graduate students in the Ji lab expect to receive training in rational drug design, synthetic organic chemistry, medicinal chemistry, chemical biology, and molecular and cell biology. (usf.edu)

In this Perspective, we discuss the recent progress in using DNA-encoded chemical libraries to interrogate complex biological targets and their potential to identify structures that elicit function or possess other useful properties. (nature.com)
Such models cannot interrogate your target within its complex cellular environment. (thermofisher.com)

Fig. 1: General scheme and key considerations in realizing functional DEL assays. (nature.com)
Fig. 5: Strategies for functional DEL assays. (nature.com)
Fig. 6: OBOC-DELs enable novel functional DEL assays. (nature.com)
Design, conduct, analyze and interpret biochemical and cellular functional assays to assess mode of action and therapeutic potential of novel small molecules. (lookforzebras.com)
G4 structural and functional studies have largely focused on DNA G4s, and the number of biological functions assigned to these motifs has grown rapidly since the discovery of their involvement in telomere biology. (grantome.com)
In AIM 3, we will use functional assays to determine the biological significance of RG4s in mRNA stability, localization and translation. (grantome.com)
We posit that using human tumor molecular and mutation data as a foundation for functional studies will lead us to novel insights about the fundamental biology of melanoma as well as provide us with inspiration for designing novel therapeutic strategies. (lu.se)

A scratch wound-healing assay was used to detect the migration of VSMCs, and immunocytochemistry and western blotting for SM22ɑ were used to evaluate phenotypic transformation. (hindawi.com)

We will discuss the structure and function of both proteins and small molecules in biological systems. (columbia.edu)
The Scientist contributes to discovery projects by designing, executing, and optimizing assays to assess biological activity of novel small molecules for cancer indications. (lookforzebras.com)

Lectures on these topics will be integrated with introductory lectures on molecular and structural biology, thermodynamics, statistics, and machine learning. (columbia.edu)

CMT also offers a cell-based assay technology based on Sentigen Biosciences (www.sentigen.com) Tango Assay System. (genengnews.com)
Sentigen Biosciences developed Tango assays for GPCR profiling of targets of relevance to pharmaceutical discovery and development. (genengnews.com)
Redistribution assays are independent of the imaging platform and have been shown to give consistent results on a number of instruments on the market, including: IN Cell 3000 (GE Healthcare), Opera (Evotec Technologies), ArrayScan (Cellomics), Pathway HT (BD Biosciences), Discovery1 (Molecular Devices), and Icyte (CompuCyte). (genengnews.com)

I find that activin mRNA is present in both cholinergic and noradrenergic targets. (caltech.edu)

In contrast to other approaches, the system improves the likelihood of a successful NGS run by not only quantifying amplifiable DNA, but also optimizing the target enrichment conditions. (qiagen.com)
The two approaches were: (i) an MGB-based assay (MGB-assay), and (ii) a SYBR Green-based assay (SYBR-assay) (Applied Biosystems). (biomedcentral.com)
We will discuss approaches for discovering and optimizing chemical tools for measuring and perturbing biological systems. (columbia.edu)
Finally, we will invite speakers to talk about aspects of personalized or stratified medicine based on systems biology approaches for better care and future drug development. (lu.se)

PCR is performed and the raw C T values are exported to a web-based data analysis module, which will quantify and qualify DNA as well as provide guidance on DNA amounts and PCR cycles needed to optimize targeted enrichment conditions. (qiagen.com)
This work not only provides insights into how pain is interpreted, but could lead to the development of new methods to detect, quantify, or target pain. (nih.gov)

Here, we describe the generation and verification of a compendium of highly specific assays that enable quantification of 99.7% of the 20,277 annotated human proteins by the widely accessible, sensitive, and robust targeted mass spectrometric method selected reaction monitoring, SRM. (nih.gov)

A real-time reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome-associated coronavirus (SARS-CoV). (cdc.gov)
What is urgently needed is precise and comprehensive targeted pathogen identification, including the capacity to detect drug-resistant mutations, for routine diagnosis to support effective treatment and control measures. (labroots.com)

Design, optimize, validate, and implement cell-based assays to assess proliferation, cell viability, and gene expression changes to support target validation and compound characterization efforts. (lookforzebras.com)

In this course, we will cover subject matter in chemical biology. (columbia.edu)
The course is intended to provide a foundation needed for advanced chemical biology research, i.e. the creation and use of chemical probes of biological processes and macromolecular function. (columbia.edu)
In addition, he played an important role in establishing the Chemical Biology group within MRL, focused on the identification of novel targets from clinically relevant disease phenotypes. (phrmafoundation.org)

In previous courses, we have guided students in the ablation of a wide variety of genes and helped them design suitable assays for their biological interests. (cshl.edu)

miR-128-5p directly targeted the 3′UTR-binding sequences of the p21 gene, negatively regulated p21 expression, and supported the proliferation of VSMCs. (hindawi.com)
Experience in mammalian cell tissue culture and cell-based assays, transfection, lentivirus transduction, stable cell line generation, viability and proliferation assays, fluorescence and luminescence based readouts. (lookforzebras.com)

The real world application of artificial intelligence methods in target identification and therapeutic discovery will be presented by key leaders in the field from pharma and biotech. (slas.org)
These methods may also incorporate simple calculated properties, for example the so-called 'Rule of 5' (5) to ensure libraries stay within sufficiently druglike areas of chemical space as well as existing structure activity relationship data describing the interaction of the chemical matter with the principle biological target, as well as related targets. (ddw-online.com)
Thus, a transition in toxicology-from a traditional reductionist paradigm towards 21st century methods based on human biology and holistic multi- omics studies-is now becoming a reality. (brill.com)
However, there is still a knowledge gap regarding the comparison of biological differences in cells obtained through different culture methods. (bvsalud.org)

For development of Redistribution assays, BioImage (www.bioimage.com) uses Aequorea victoria GFP and other fluorescent proteins fused to translocating targets of interest. (genengnews.com)
Proteins are the primary class of biological macromolecules and serve to carry out most cellular functions. (columbia.edu)
A river of biological information flows just beneath the outermost layers of your skin, in which a hodgepodge of proteins squeeze past each other through the interstitial fluid surrounding your cells. (theceliacscene.com)
The aquaporins (AQPs) form a family of integral membrane proteins that facilitate the movement of water across biological membrane by osmosis, as well as facilitating the diffusion of small polar solutes. (lu.se)
However, recent studies have suggested that targetting the trafficking of AQP proteins to the plasma membrane is a viable alternative drug target to direct inhibition of the water-conducting pore. (lu.se)

Most recently, students have targeted autism genes, thyroid genes and immune modulators, several of which have already led to publications. (cshl.edu)
miR-128-5p targets the tissue inhibitor of metalloproteinases (TIMPs), silent information regulator 2 (SIRT2), peroxisome proliferator-activated receptor (PPAR), and p21 genes, which are linked to the behaviours of VSMCs. (hindawi.com)
Synthetic antisense DNAs are used to hybridize to complementary sequences in target RNAs or DNAs to effect the functioning of specific genes for investigative or therapeutic purposes. (genomicglossaries.com)
Use the PrimePCR lookup tool to find assays and panels for your genes of interest. (bio-rad.com)
The association of these target genes with thyroid tumorigenesis, stages, subtypes, and survival rates were analyzed. (bvsalud.org)

The inherent complexity of biological systems is a challenge that is expected to be overcome by computational modeling of biological systems. (brill.com)

Redistribution assays can be multiplexed by either expressing two targets in the same cell line labeled with different colors, or by mixing different assay cell lines directly in the microtiter plate well. (genengnews.com)
Difficulties inherent in recreating relevant biological models within the confines of a microtiter plate often limit early discovery efforts to simplified assay systems using purified components. (thermofisher.com)

We readily detected off-target alterations induced by four out of six RGENs targeted to endogenous loci in human cells by examination of partially mismatched sites. (cdc.gov)

However, recent innovations demonstrate the capability of DELs to operate in the complex milieu of biological systems. (nature.com)
Taught by research scientists active in various of systems biology, the course is highly interdisciplinary and rooted in recent research, with a soft focus on cancer. (columbia.edu)
But the research aims to create assay systems for screening drugs for autism as well as other brain disorders," Sumantra Chattarji, professor of neurobiology at the National Centre for Biological Sciences, who heads the Centre, told Deccan Herald. (deccanherald.com)
GeneGo, Inc., the leading systems biology tools company, announced today the release of MetaMiner Cystic Fibrosis, a first of its kind software and database platform designed to help advance drug discovery for cystic fibrosis, a life-threatening genetic disease. (cff.org)
Cystic Fibrosis Foundation Therapeutics, Inc., a non-profit affiliate of the Cystic Fibrosis Foundation, provided funding and guidance to the project with the goal of leveraging systems biology technology and accumulating data in order to accelerate drug discovery for this disease. (cff.org)
GeneGo develops systems biology technology for life science research. (cff.org)
The term systems toxicology is derived from systems biology and could be defined as the study of biological systems, using omics technologies, with a focus on the mechanisms underlying complex biological processes, their interactions and perturbations in response to a toxin combined with mathematical data integration and modeling. (brill.com)
Systems toxicology, therefore, aims at understanding and exploring the way that different biological components are orchestrated as an ensemble in cells, tissues, and organisms. (brill.com)
This study systematically compares the biological properties of hUC-MSCs amplified through planar culture and microcarrier-bioreactor systems. (bvsalud.org)
The potential spectrum of bioterrorism ranges from hoaxes and actual use of agents by individuals or groups against others, to state-sponsored terrorism that employs biological warfare (BW) agents and delivery systems that can produce mass casualties. (medscape.com)

Early assays based on conventional designs that required postamplification product processing (e.g., gel electrophoresis), were time-consuming and prone to false-positive results from amplicon contamination. (cdc.gov)
Conversely, real-time RT-PCR assays based on detecting and quantifying a fluorescent signal generated during amplification do not require postamplification processing and therefore eliminate one potential avenue for template contamination. (cdc.gov)
This assay may also be used to compare relative levels of gDNA contamination present in different samples to determine if PCR results may be affected. (bio-rad.com)

however, despite its prevalence in FL and other cancers, the role of CA125 in tumor biology and immunity has been only recently elucidated. (spandidos-publications.com)

A variety of RT-PCR assays were developed during the epidemic for SARS-CoV ( 1 , 5 - 8 ), including a commercial ready-to-use RT-PCR kit (Artus Biotech, Hamberg, Germany). (cdc.gov)
Biotech/Biochem & PhD Biological Sciences/Biotech jobs for eligible candidates at CSIR-IICB. (biotecnika.org)

The session on super-resolution microscopy will explore its emergence from basic research applications towards exciting new applications in industrial scale screening of biological events at unparalleled sub-cellular scales. (slas.org)
Find or custom design the right target-specific assays and panels to research your biological targets of interest. (qiagen.com)
Bio-ITWorld.com, Weekly Update newsletter and News Bulletins cover the application of informatics, IT and computer science in biomedical research and drug discovery. (bio-itworld.com)
The Malachite Green Phosphate Assay Protocol reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (urokinases.com)
Scientists hope to unravel the complex brain circuitry underlying autism at a new Indo-UK research centre in Bangalore that aims to eventually develop better diagnostic techniques and drug targets for a host of brain disorders. (deccanherald.com)
Each gene target within a pathway was assigned a score based on the frequency of differential expression and its research significance. (bio-rad.com)
Understanding the scope of RGEN-mediated off-target effects in human and other eukaryotic cells will be critically essential if these nucleases are to be used widely for research and therapeutic applications. (cdc.gov)
During World War II, the Japanese operated a secret biological warfare research facility in Manchuria and carried out human experiments on Chinese prisoners. (medscape.com)

Although Maraffini and colleagues7 recently performed a systematic investigation of Cas9 RGEN specificity in bacteria, the specificities of RGENs in human cells have not been extensively defined and, to our knowledge, bona fide off-target mutations induced by Cas9 have not been identified in any eukaryotic cell or organism. (cdc.gov)
To begin to define the specificity determinants of RGENs in human cells, we sought to perform a large-scale test in which we assessed the effects of systematically mismatching various positions within multiple gRNA/target DNA interfaces. (cdc.gov)

Every PCR primer pair was experimentally validated using Bio-Rad's iScript™ advanced cDNA synthesis kit and SsoAdvanced™ SYBR ® Green supermix. (bio-rad.com)

The pharmacology of known ligands of the target can also be used to inform the development of libraries from chemical scaffolds. (ddw-online.com)
Xenopus is remarkable for studying development and disease, including birth defects, cancer, and stem cell biology. (cshl.edu)
We experimented with cells division-arrested with low level mitomycin C treatment and demonstrated that they perform with better consistency than non-division arrested counterparts in high-content screening imaging assays, says Zhong Zhong, vp of development at CMT. (genengnews.com)
However, the downside of targeted therapies is development of resistance. (lu.se)

In response to the SARS public health emergency, we developed and evaluated a TaqMan real-time RT-PCR assay based on three distinct targets in the SARS-CoV genome for rapid deployment to the National Laboratory Response Network for Bioterrorism (LRN) ( http://www.cdc.gov/programs/bio.htm ). (cdc.gov)

This expanding panel of cell-based receptor assays, which currently includes 54 targets, is being offered by CMT for custom compound profiling to evaluate compound selectivity over a wide range of likely GPCR targets. (genengnews.com)

The assay uses next-generation DNA sequencing (NGS) along with Fusion's novel DNA hybridization technology, QuantumProbes, to enrich bacterial/viral/fungal genomes prior to sequencing. (labroots.com)

QIAseq DNA QuantiMIZE Kits are intended for molecular biology applications. (qiagen.com)
The QIAseq Library Quant System is intended for molecular biology applications. (qiagen.com)

In this article, we propose that chemical libraries which target the ubiquitin system are the missing keys to unlock the therapeutic potential of ubiquitin system drug discovery. (ddw-online.com)
Consequently, small-molecule drug discovery usually involves an iterative process of molecular design, chemical synthesis, biological assay and data analysis feeding directly into the next cycle, but this process always needs a chemical starting point. (ddw-online.com)
Future breakthroughs in these aspects are expected to catapult DEL to become a momentous technology platform not only for drug discovery but also to explore fundamental biology. (nature.com)

Each pathway belongs to one or more general biological categories such as cancer. (bio-rad.com)

A variant of the real-time format, based on TaqMan probe hydrolysis technology (Applied Biosystems, Foster City, CA), has been shown to provide sensitive, specific, and quantifiable results in viral diagnostic assays ( 9 ) and has been used successfully to study emerging virus infections ( 10 , 11 ), including SARS ( 6 , 12 ). (cdc.gov)

In competitive immunoassay, the target BChE in a sample competes with the BChE immobilized on the MBs to bind to the limited sites of anti-BChE antibody labeled with quantum dots (QD-anti- BChE), followed by stripping voltammetric analysis of the bound QD conjugate on the MBs. (cdc.gov)

Leading experts in disease biology will present new strategies for identifying, investigating and validating challenging target mechanisms in case studies. (slas.org)

Biological warfare became more sophisticated against both animals and humans during the 20th century. (medscape.com)

These fluorescent signals can be read in a timeresolved manner to reduce assay interference and increase data quality. (thermofisher.com)

Upon IkappaBalpha degradation, liberated NFkappaB translocates to the nucleus to activate target gene expression. (thermofisher.com)

P rotein kinases, on the other hand, have become one of the most important classes of drug targets for the pharmaceutical industry over the last decade, following on from the exploitation of kinase-focused libraries for at least the last two decades. (ddw-online.com)

We are currently undertaking studies to understand the biology as well as defining novel therapeutic targets in the two lineage states of melanoma. (lu.se)

Integrating LC-MS/MS molecular networking and bioassay-guided fractionation enabled the targeted isolation of a new and bioactive cyclic octapeptide, samoamide A (1), from a sample of cf. (henryford.com)
Combining bioassay screening with the cheminformatics strategy of LC-MS/MS molecular networking as a discovery tool expedited the targeted isolation of a natural product possessing both a novel chemical structure and a desired biological activity. (henryford.com)

Author Manuscript previously described by our lab15 that enables rapid quantitation of targeted nuclease activities (Fig. 1a). (cdc.gov)

Anatomy3

Organisms2

Chemicals and Drugs7

Analytical, Diagnostic and Therapeutic Techniques and Equipment10

Phenomena and Processes14

Disciplines and Occupations7

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture1

Information Science7

Biomarkers3

Vitro2

Detection6

High-throughput7

Pathways7

Quantitative6

Genomic6

Cellular Assays2

Biochemical assays3

QPCR2

Reagents3

Real-Tim5

Biochemistry6

Protein12

Intracellular2

Sensitivity3

Compounds8

Clinical7

Chemistry7

Interrogate2

Functional7

Phenotypic1

Small molecules2

Structural Biology1

Biosciences3

MRNA1

Approaches4

Quantify2

Quantification1

Detect2

Optimize1

Chemical biology3

Suitable1

Proliferation2

Methods4

Proteins5

Genes5

Computational1

Microtiter plate2

Loci1

Systems10

Contamination3

Tumor biology1

Biotech2

Research8

Specificity2

Primer1

Development4

Genome1

Selectivity1

Genomes1

Intended for molecular biology applications2

Drug discovery3

Pathway1

Viral1

Immunoassay1

Mechanisms1

Humans1

Fluorescent1

Gene expression1

Pharmaceutical1

Therapeutic Targets1

Isolation2

Enables rapid1