• Nonlinear current-voltage relationships in cultured macrophages. (rupress.org)
  • Organelle relationships in cultured 3T3-L1 preadipocytes. (silverchair.com)
  • Studies of viral attachment to cultured gastrointestinal epithelial cells (Caco-2) using recombinant virus-like particles or infectious noroviruses indicate that specific histo-blood group antigens play a key role in the attachment of the virus to the host cells ( 13 - 17 ). (cdc.gov)
  • Multiple intestinal atresia with combined immune deficiency (MIA-CID) is an autosomal recessive syndrome due to mutations in the TTC7A gene implicated in the polarization of intestinal and thymic epithelial cells. (bvsalud.org)
  • Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
  • Gottlieb Haberlandt first pointed out the possibilities of the culture of isolated tissues, plant tissue culture. (wikipedia.org)
  • He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
  • When cells from these tissues are processed and cultured, the first monolayer is referred to as Primary culture. (lifeeasy.org)
  • Cells grown on porous collage-coated beads under fluid shear conditions in rotating wall vessel bioreactors differentiate into 3-D architectures resembling both the morphologic and physiologic function of in vivo tissues. (cdc.gov)
  • The design of the RWV bioreactor is based on the principle that organs and tissues function in a 3-D environment and that this spatial context is necessary for development of cultures that more realistically act like in vivo tissues and organs ( 25 ). (cdc.gov)
  • Investigations include smears, biopsy, staining with periodic acid-Schiff (PAS) or Gomori methenamine silver, culture of the affected tissues, polymerase chain reaction (PCR), serodiagnosis (sometimes), physical examination, and chest radiography. (medscape.com)
  • Tissues and Cells. (lu.se)
  • Normal proliferative tissues contain multipotent stem cells (SCs) that drive tissue turnover. (medscape.com)
  • According to the cancer stem cell (CSC) concept, the cellular hierarchy in normal tissues is preserved in a number of hematological and solid malignancies. (medscape.com)
  • Objective: We developed a novel, streamlined protocol for generating chondrocytes from bone marrow-derived mesenchymal stem cells (BMSCs) in a 3D culture system that offers. (lu.se)
  • Objective: We developed a novel, streamlined protocol for generating chondrocytes from bone marrow-derived mesenchymal stem cells (BMSCs) in a 3D culture system that offers significant implications for the study of cartilage biology and the discovery of potential therapeutic interventions for cartilage-related and associated disorders. (lu.se)
  • To meet different customer needs Minerva Biolabs has developed a range of Venor®GeM mycoplasma detection assays using conventional PCR or qPCR methods which enable rapid and reliable identification of mycoplasma in cell cultures, culture supernatants and tissue culture media. (cambio.co.uk)
  • Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
  • The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows. (wikipedia.org)
  • In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). (wikipedia.org)
  • The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. (wikipedia.org)
  • In 1885 Wilhelm Roux removed a section of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the basic principle of tissue culture. (wikipedia.org)
  • Ross Granville Harrison, working at Johns Hopkins Medical School and then at Yale University, published results of his experiments from 1907 to 1910, establishing the methodology of tissue culture. (wikipedia.org)
  • In modern usage, "tissue culture" generally refers to the growth of cells from a tissue from a multicellular organism in vitro. (wikipedia.org)
  • Because of the lack of suitable tissue culture or animal models, the true nature of norovirus pathogenesis remains unknown. (cdc.gov)
  • Once founded, the cell line can be propagated into several flasks and frozen. (atcc.org)
  • Biological variance is introduced by prolonged culturing (react to differences of FCS, culture flasks, Selection by splitting, etc. (stackexchange.com)
  • Our results demonstrate that the highly differentiated 3-D cell culture model can support the natural growth of human noroviruses, whereas previous attempts that used differentiated monolayer cultures failed. (cdc.gov)
  • This simple pellet-based 3D culture system allows for cell aggregation and spheroid formation, facilitating cell-cell and cell-extracellular matrix interactions that better mimic the in vivo cellular environment compared with 2D monolayer cultures. (lu.se)
  • When using cell lines or mouse strains, have accurate records of their origin and genetic background, and how they are identified and maintained. (duke.edu)
  • Hayflick, L. & Moorhead, P. S. The serial cultivation of human diploid cell strains. (nature.com)
  • Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture. (wikipedia.org)
  • Discover best practices how to subculture adherent and suspension cultures. (atcc.org)
  • It took off in 1958 when researchers discovered that fetal bovine serum (FBS) was an additive that could make cells thrive, both normal and cancerous. (lu.se)
  • Who has experience with mammalian cells? (bio.net)
  • Interactions between light and various cells in cultures, such as bacteria or mammalian cells, are widely applied for optical sensors and optofluidic systems. (mdpi.com)
  • The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
  • This essential laboratory process covers the thaw, growth, propagation, and cryopreservation of cells that have been isolated from a donor organism. (atcc.org)
  • The founding laboratory should also institute the seed stock principle to bank the cells. (atcc.org)
  • As an efficient contamination control measure we recommend frequent routine testing of cell culture materials with Venor®Gem Mycoplasma Detection Kits . (cambio.co.uk)
  • Venor®GeM Classic allows fast, reliable and time-saving routine monitoring of mycoplasma contamination in cell culture supernatants, media and biopharmaceuticals in research and industry by conventional PCR. (cambio.co.uk)
  • 3 h) and reliable screening of cell culture supernatants for mycoplasma contamination. (cambio.co.uk)
  • These comprise basic cell culture technology, e.g. cell cultivation and expanding cell cultures, but also authentication and contamination tests. (dsmz.de)
  • Because of the invaluable nature and numerous applications of cell lines in life science research, it is critical that they are handled carefully to not only avoid contamination but to protect human safety. (atcc.org)
  • What so many people have been working on for so long was surprisingly easy, after all," says Stina Oredsson, biology researcher at Lund University. (lu.se)
  • TOKYO, March 19, 2021― FUJIFILM Corporation (President: Kenji Sukeno) has announced the selection of Holly Springs, North Carolina as the location for its new large-scale cell culture production site in the United States. (fujifilm.com)
  • 2021. Sexual Dimorphism in Cancer: Insights from Transcriptional Signatures in Kidney Tissue and Renal Cell Carcinoma. (who.int)
  • This vaccine was made possible by the cell culture research of John Franklin Enders, Thomas Huckle Weller, and Frederick Chapman Robbins, who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)
  • They are derived from primary cell cultures developed from a particular type of tissue such as kidney or lung, which is of embryonic origin. (lifeeasy.org)
  • 20 ) reported production of Norwalk virus particles (norovirus GI.1, the prototype strain of this genera from Norwalk, Ohio) after transinfection of cultured kidney cells. (cdc.gov)
  • Best practices for reviving cells from cryopreservation are critical to ensuring unambiguous experimental results in basic research and industrial processes. (atcc.org)
  • These best practices form the basis of several cell culture guides , webinars , and video tutorials that detail the initiation, expansion, and cryopreservation of continuous cell lines, primary cells, hTERT-immortalized primary cells, and organoids. (atcc.org)
  • High quality but affordable diagnostic kits for the detection of microbial contaminants in cell cultures, biopharmaceutical products, clinical samples and water. (cambio.co.uk)
  • The previously announced investment of more than 200 Billion yen (2 Billion USD) to establish the largest end-to-end cell culture biopharmaceutical CDMO *1 facility in North America will create 725 highly-skilled jobs in the area by the end of 2028. (fujifilm.com)
  • Additional growth factors and hormones are added to promote growth or attachment to the culture vessel, often via undefined adjuncts such as serum. (atcc.org)
  • Most freshly isolated, primary cell cultures undergo senescence, the process by which cells stop dividing after undergoing a certain number of cell divisions. (atcc.org)
  • Many scientific journals currently do not accept submissions of cell-based studies without evidence that mycoplasma testing has been performed. (cambio.co.uk)
  • Be a part of the movement to raise credibility in science and order your cell line authentication and mycoplasma testing services today. (atcc.org)
  • Recently, the first in vitro norovirus cell culture model was reported for a virus that infects mice ( 18 , 19 ). (cdc.gov)
  • Background: The success of in vitro-fertilization (IVF) cycles is determined in large part by the quality of embryo cleavage, which in turn, is dependent on the quality of the embryo culture media (CM). Many factors can influence the quality of embryo CM, one of which is the levels of Cell Free Deoxyribonucleic acid (DNA). (who.int)
  • Described here are some of the general requirements of in vitro test methods for skin sensitization, and progress that has been made in developing suitable approaches with particular emphasis on the utility of dendritic cell culture systems. (cdc.gov)
  • 3D models provide a more accurate representation of cells in vivo and are more stable than 2D cultures, making them very useful for long-term studies. (selectscience.net)
  • In this study, we developed an EV production method utilizing in vivo -mimicking extracellular matrix-based 3D cultures, which allows tracking of EVs over the course of invasive development of tumor organoids. (biorxiv.org)
  • Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
  • Multiple methods of isolating cells for culture exist. (atcc.org)
  • Methods: We developed a streamlined protocol for generating chondrocytes from BMSCs in a 3D culture system using an "in-tube" culture approach. (lu.se)
  • Methods: This prospective study was conducted with 96 spent CM from patients undergoing IVF cycle, in order to determine relationships of Cell-free DNA levels in embryo CM with embryo cleavage quality on day 3. (who.int)
  • When doing studies with human samples, including iPS (induced pluripotent stem) cells or embryonic stem cells, be particularly careful about possible restrictions on their use and terms of IRB (Institutional Review Board) approvals. (duke.edu)
  • In 1907 the zoologist Ross Granville Harrison demonstrated the growth of frog embryonic cells that would give rise to nerve cells in a medium of clotted lymph. (wikipedia.org)
  • With over 95 years of expertise with cell and microbial cultures, ATCC has acquired and developed a vast body of best practices to aid researchers at all levels of proficiency to maximize the return on their biomaterials investment. (atcc.org)
  • Using this method, combined with proteomic profiling, we show that PC3 human prostate cancer organoids secrete EVs with previously undefined protein cargo, which substantially differs from EV cargo of 2D cultured cells. (biorxiv.org)
  • Senescent cells are characterized by an upregulation of proinflammatory cytokines, which is termed the senescence-associated secretory phenotype (SASP). (nature.com)
  • Raw RNA-seq data for RAS-induced senescence and control conditions in IMR90 cells were downloaded from GEO (under accession code GSE74324 ) 41 . (nature.com)
  • This method ensures the protection of original stock and the consistent availability of low-passage vials of cells via the creation of a master cell bank and working cell banks. (atcc.org)
  • If you want to do it as close to properly as you can, you should get up 3 separate vials of your cells and maintain/use them individually. (stackexchange.com)
  • The neurons were grown with or without non-neural cells, simply by the addition or deletion of bicarbonate during growth in culture. (rupress.org)
  • The cells are generally grown in plastic or glass vessels. (atcc.org)
  • Experimental design: Cells are grown, counted, and seeded in 96-well plates. (stackexchange.com)
  • Female-founded food tech Orbillion Bio recently raised US$5M in its seed round for the development of its cruelty-free lab-grown alternatives to 'heritage meats' like wagyu beef, elk, sheep, and American bison. (bigideaventures.com)
  • Directory of Toll Fermentation and Cell Culture Facilities, Fourth (2001) Edition Where can we produce cGMP biopharmaceuticals for clinical trials? (bio.net)
  • Given this background, it is the goal of the Department of Cell Biology to foster a supportive and inclusive work environment in which the guiding principles of scientific integrity, innovation, open communication, training, career advancement and discovery research can flourish. (duke.edu)
  • Cell culture techniques were advanced significantly in the 1940s and 1950s to support research in virology. (wikipedia.org)
  • The Cell Culture Facility (CCF) located in the Life Science Laboratories supports research by providing space, equipment, cell cultures (plant and mammalian), media, supplies and training on a recharge basis for both academic labs and corporate partners. (umass.edu)
  • Starter cultures and growth media may be purchased from the Collection for academic or commercial research with no IP or licensing restrictions. (umass.edu)
  • Researchers from Lund have developed a completely animal-free preparation for cell culture, a medium that can be used to grow human cells, including for cancer research. (lu.se)
  • This has sparked a lot of interest in many parts of Europe and also with the US Food and Drug Administration (US FDA), not least because it can be used to grow normal cells but also in cancer research. (lu.se)
  • Annual Research & Review in Biology. (who.int)
  • Winnie Svendsen, Professor at DTU will present her research about nanostructures in bio-analytical applications. (lu.se)
  • In this talk I will introduce the work we do in my research group involving nanostructured surfaces, nanoparticles, nanopillars and nanotubes for use in bio-analytical applications. (lu.se)
  • WPI's versatile master's degree program in Biology and Biotechnology will give you the rigorous knowledge, practical skills, and hands-on experience needed to launch a successful career. (wpi.edu)
  • Regarding cell lines, yes, all the cells came from the same lot of the same ATCC catalogue entry (albeit different 'break-outs' from frozen stocks). (stackexchange.com)
  • Add 375 ml of sterile WFI quality cell culture grade water to a 1000 ml flask. (cdc.gov)
  • Normal multipotent tissue stem cells (SCs) are the driving force behind tissue turnover and repair. (medscape.com)
  • At this point, the creator of the cell line must perform some form of authentication and characterization that identifies it as a unique cell line. (atcc.org)
  • As members of the Department of Cell Biology we are all greatly privileged to be able to pursue our dreams of discovering new knowledge, widening horizons, and improving the well-being of future generations, both in our own communities and throughout the world. (duke.edu)
  • The Department has therefore posted to the website the Scholarly Culture and Accountability Plan (SCAP) outlined below. (duke.edu)
  • Dr. Mains' present address is the Department of Biology, University of Oregon, Eugene, Oregon 97403. (rupress.org)
  • The Department of Human and Animal Cell Lines offers a broad range of cell culture related services. (dsmz.de)
  • Growing viruses in cell cultures allowed preparation of purified viruses for the manufacture of vaccines. (wikipedia.org)
  • Cell culture is the most widely used method for the propagation of viruses. (lifeeasy.org)
  • Cytopathic effect and norovirus RNA were detected at each of the 5 cell passages for genogroup I and II viruses. (cdc.gov)
  • 3D Cell Culture: Tumor-specific cancer treatments, org. (selectscience.net)
  • arations verified the presence of cancer cells in all samples. (lu.se)
  • The cancer stem cell theory holds that tumors also contain stem-like cells that drive tumor growth and metastasis formation. (medscape.com)
  • Here, the authors review the novel insights that these studies have provided and present a comprehensive strategy for the use of proteomics in studying cancer SC biology. (medscape.com)
  • The CSC is defined as a cancer cell with self-renewing capacity that can regenerate the original tumor in all its differentiated heterogeneity. (medscape.com)
  • Cancer stem cell-associated proteins revealed by proteomics. (medscape.com)
  • As in hepatocytes and absorptive cells of the intestine, this spatial relationship of ER and plasmalemma suggests a role for rosettes in the uptake of exogenous lipid precursors. (silverchair.com)
  • Developing an accessible and cost-effective model to generate viable chondrocytes and to assess their response to different bioactive compounds can significantly advance our knowledge of cartilage biology and contribute to the discovery of novel therapeutic approaches. (lu.se)
  • In this webinar, delve into the exciting world of correlative workflows in structural biology rese. (selectscience.net)
  • Watch our on-demand webinar to explore the development of a new STR profiling method for the authentication of mouse cell lines. (atcc.org)
  • BFF staff have experience with a wide range of cell types and are familiar with production using stable cell lines or transient transfection. (uga.edu)
  • For robust NK cell expansion under xeno-free conditions. (bio-techne.com)
  • Plants produce over 200,000 different kinds of small organic chemicals called "secondary" or "specialized" metabolites that serve many important roles in plant biology, and have been used in various industries including pharmaceuticals, cosmetics, flavors, dyes, dietary supplements and fragrances. (umass.edu)
  • The injectable polio vaccine developed by Jonas Salk was one of the first products mass-produced using cell culture techniques. (wikipedia.org)
  • R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. (bio-techne.com)
  • ACD branded products will be available on bio‑techne.com in the near future. (bio-techne.com)
  • Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com . (bio-techne.com)