• Apoptosis was induced by dealing with 4 × 107 Jurkat T cells with etoposide (10 μM) camptothecin Podophyllotoxin (10 μg/ml) 7 Podophyllotoxin (UCN-01) (5 μM) or staurosporine (STS) (1 μM) (all from Sigma-Aldrich Co. St. Louis MO). (bioinf.org)
  • Pyroptosis is a form of lytic regulated cell death (RCD) first described by Zychlinsky and colleagues in 1992 as apoptosis, but later renamed as pyroptosis in 2001 by Cookson and Brennan to reflect its inflammatory nature. (bio-rad-antibodies.com)
  • Its pro-inflammatory nature distinguishes it from other forms of RCD, like apoptosis and necroptosis, and it is in fact the most immunogenic of all the cell death mechanisms. (bio-rad-antibodies.com)
  • Measured by its ability to inhibit Fas Ligand-induced apoptosis of Jurkat human acute T cell leukemia cells. (bio-techne.com)
  • Yedjou C, Izevbigie E, Tchounwou P. (2013) Vernonia amygdalina-Induced Growth Arrest and Apoptosis of Breast Cancer (MCF-7) Cells. (nexcelom.com)
  • His Cell biology research includes elements of Apoptosis, Caspase 1 and Proteolysis. (research.com)
  • As a demonstration of the utility of this technique, a few applications of transiently adhering Jurkat cells are shown which would be otherwise difficult with freely suspended cells, such as increased gene delivery, confocal-based apoptosis detection, and real-time electric-field effect monitoring in Jurkat cells. (lsu.edu)
  • PS becomes surface exposed during apoptosis and cell activation. (cancerhugs.com)
  • Current literature also reveals PS-binding proteins that inhibit phagocytosis of apoptotic cells, like the well known apoptosis marker Annexin V [20], or high mobility group protein-1 [21]. (cancerhugs.com)
  • Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. (thermofisher.com)
  • Extensive lymphocyte severe infections is a massive loss of lymphocytes, den- apoptosis has also occurred in humans and animals infect- dritic cells, gastrointestial epithelial cells, and other cell ed with several exotic agents, including Bacillus anthracis , types through apoptosis, or programmed cell death. (cdc.gov)
  • Prevention of lymphocyte apoptosis, same process by which these cell populations are regulat- through either genetic modification of the host or treatment ed during normal health ( 1 , 2 ). (cdc.gov)
  • acquired defects in the control of apoptosis may lead to a Apoptosis, or programmed cell death, is the method variety of disease states. (cdc.gov)
  • For example, excessive inhibition by which tissue remodeling takes place during normal of apoptosis is an underlying mechanism of cancer, while growth and development and the physiologic mechanism an inappropriate increase is seen in some neurodegenera- by which labile cell populations such as gastrointestinal tive diseases and other conditions. (cdc.gov)
  • Lysate (reverse phase) arrays contain complex samples, such as cell or tissue lysates, that are printed on an array surface and interrogated with antibodies. (tovi.io)
  • Western Blot analysis of (A) RAW264.7 and (B) NIH-3T3 cell lysate using Active/Pro-Caspase 3 Monoclonal Antibody (31A1067) (Product # MA1-91637). (thermofisher.com)
  • Positive Control: Staurosporine-treated HeLa or Jurkat cell lysate. (thermofisher.com)
  • Cell viability was examined by life/membrane staining reporting comparable viability on planar and nanowire substrates. (lu.se)
  • Imaging the hybrid interface reveals a wrapping of the cell membrane around the very nanowire tip. (lu.se)
  • TTYH family members form tetrameric complexes at the cell membrane. (cuni.cz)
  • Both endogenous and enhanced green fluorescent protein (EGFP)-fused Gβ2 and Gγ2 proteins were detected in the nucleus at baseline, whereas a fraction of EGFP-Gβ2 and DsRed2-GR comigrated to the nucleus or the plasma membrane, depending on the exposure of cells to dexamethasone or somatostatin, respectively. (rupress.org)
  • The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms. (creative-biolabs.com)
  • Interestingly, Heminecrolysin was found to induce PS exposure on human nucleated Jurkat T cells, to stimulate secretion of the pro-inflammatory (TNF-α, IL-6), and anti-inflammatory (IL-10) cytokines by human monocytes, and to provoke a disseminated intravascular coagulation on chick embryo chorioallantoic membrane model system. (hal.science)
  • A successful cryopreservation procedure involves the use of a solution that maintains sperm inactivity without compromising the cell membrane integrity and in combination with cryoprotectants, protecting sperm from injury during the freeze-thawing cycle. (scialert.net)
  • Other than propidium iodide, other fluorescent membrane exclusion dyes can also be used to measure cell viability. (nexcelom.com)
  • 1978. Altered erythrocyte membrane proteins in sickle cell patients associated with the severity of the disease. (mote.org)
  • We describe the isolation of Jurkat cell-specific membrane rafts using 2% Triton X-100. (upenn.edu)
  • Membrane proteins implicated in long-chain fatty acid uptake by mammalian cells: CD36, FATP and FABPm. (springer.com)
  • The cell pellet was after that resuspended at a thickness of 107 cells/ml in CM and cultured at 37°C in 5% CO2. (bioinf.org)
  • The pan-caspase inhibitor for 5 min was utilized to pellet out the treated cells. (bioinf.org)
  • The cell-free supernatant was after that centrifuged at 16 0 30 min within a microcentrifuge (Denville 2600 Denville Scientific Inc. Metuchen NJ) to isolate the MP pellet. (bioinf.org)
  • Quickly the MP pellet isolated from 107 Jurkat cells was resuspended in 250 μl of BD Cytofix/Cytoperm straight? (bioinf.org)
  • Remove and discard 10 ml of old growth medium from above the cell pellet. (neb.com)
  • Add 10 ml of fresh complete growth medium and gently resuspend cell pellet by pipetting up and down 2-3 times. (neb.com)
  • DNA standards and GAPDH-2 primers were used to analyze DNA from Fixed Jurkat Cell Pellet samples (equivalent to 100,000 Jurkat cells). (activemotif.com)
  • Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance. (creative-biolabs.com)
  • Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure. (creative-biolabs.com)
  • Additional analysis characterized the occurrence of this transient cell adhesion, including attachment rate, reversibility of attachment, and viability and preservation of phenotype in cells during and after attachment. (lsu.edu)
  • Activation of PI3K is indispensable for interleukin 7-mediated viability, proliferation, glucose use, and growth of T cell acute lymphoblastic leukemia cells. (istanbul.edu.tr)
  • Our study shows that Jurkat human T cells have a molecule that reacts with both anti-CR2 antibodies and the third component of complement, C3. (nih.gov)
  • Immunoprecipitation experiments with anti-CR2 antibodies and SDS-PAGE analysis reveal a protein with an apparent molecular mass of 155 kDa which is higher than the one seen in B cells. (nih.gov)
  • As experts in innate immunity and cell culture, we offer a large selection of products including PRR ligands , cell lines , selective antibiotics , antimicrobial agents , vaccine adjuvants , and antibodies . (invivogen.com)
  • More recently, these cells have been exploited in imaging studies to identify key players in immunological synapse (IS) assembly in superantigen-specific conjugates and to track the dynamics of signaling molecules on glass surfaces coated with activating anti-CD3 antibodies. (ox.ac.uk)
  • These receptors bind to the Fc portion of IgG antibodies which then activates the NK cell for antibody-dependent cell-mediated cytotoxicity. (creative-biolabs.com)
  • ATAD2 selectively binds to acetylated histone 4 and has been shown to regulate a number of cellular processes including cell proliferation. (bio-rad-antibodies.com)
  • Finally, we studied the effects of arsenide trioxide and 5-azacitidine on the proliferation of leukemia cells and the TGF- β signaling pathway. (hindawi.com)
  • At the same time, arsenide trioxide and 5-azacitidine are associated with the inhibition of cellular proliferation of acute leukemia cells and also promote the elevated expression of TGF- β signaling pathway-linked proteins, including TGF- β , Smad2, Smad3, and Smad4. (hindawi.com)
  • Stimulation of T cells through the T-cell receptor results in the activation of a series of signaling pathways that leads to the secretion of interleukin (IL)-2 and cell proliferation. (cdc.gov)
  • Changes in dietary fatty acids, specifically the polyunsaturated fatty acids of the ω-3 and ω-6 families and some derived eicosanoids from lipoxygenases, cyclooxygenases, and cytochrome P-450, seem to control the activity of transcription factor families involved in cancer cell proliferation or cell death. (springer.com)
  • SATB1 is expressed by thymocytes (especially single positive CD4+ thymocytes), T and B lymphocytes, NK cells, and macrophages. (bdbiosciences.com)
  • B cells were gated on singlet lymphocytes as CD19+ cells. (bioxorio.com)
  • These findings suggest that inter- the apoptotic destruction of lymphocytes and dendritic ventions aimed at reducing the extent of immune cell apop- tosis could improve outcomes for a variety of severe cells could have a particularly adverse effect on disease human infections, including those caused by emerging outcome. (cdc.gov)
  • The mechanisms of action by which RTX kills FL cells include complement-dependent cytotoxicity (CDC) ( 3 ), antibody-dependent cellular cytotoxicity (ADCC) ( 4 , 5 )-herein referred to as 'effector function' mechanisms-as well as CD20 signaling effects ( 6 ). (spandidos-publications.com)
  • Here we have explored whether upregulating the low levels of endogenous LFA-1 expression on Jurkat E6.1 cells through transduction with CD11a- and CD18-encoding lentiviruses can improve IS architecture. (ox.ac.uk)
  • Methods The MR1-restricted T cell clone, MC.7.G5 (7G5.TCRT), with reported cancer specificity and pan-cancer activity, 1 was cloned and expressed via lentiviral transduction in Jurkat E6.1 TCRαβ- β2M- CD8+ NF-κB:CFP NFAT:eGFP AP-1:mCherry cells or in human donor T cells (with or without CD8 or after knockout of the TRBC1 and 2 genes with CRISPR Cas9). (bmj.com)
  • Thus, PKCθ represents an additional member of the PKC family, and its predominant expression in hematopoietic cells suggests that it may play a role in signal transduction and growth regulatory pathways unique to these cells. (bgu.ac.il)
  • Prof Hampton's research is focused on how understanding how cells sense and respond to oxidants, and how these pathways might be modulated to prevent and treat human disease. (otago.ac.nz)
  • Human immunodeficiency virus (HIV) infects helper T-cells by binding to the CD4 and CCR5/CXCR4 receptors for viral entry. (gmu.edu)
  • We sought to better understand the biology of IL32 in human melanoma. (biomedcentral.com)
  • We analyzed RNA sequencing data from 53 in-house established human melanoma cell lines and 479 melanoma tumors from The Cancer Genome Atlas dataset. (biomedcentral.com)
  • In order to study the transcriptional regulation of IL32 in these cell lines, we cloned up to 10.5 kb of the 5′ upstream region of the human IL32 gene into a luciferase reporter vector. (biomedcentral.com)
  • A significant proportion of established human melanoma cell lines express IL32, with its expression being highly correlated with a dedifferentiation genetic signature (high AXL/low MITF). (biomedcentral.com)
  • However, the role of IL32 in human melanoma cells is less well understood. (biomedcentral.com)
  • The human melanoma cell lines were established from patient biopsies. (biomedcentral.com)
  • Our findings illustrate a key role of the EGLN-pVHL pathway in suppressing RIPK1 activation under normoxic conditions to promote cell survival and a model by which hypoxia promotes RIPK1 activation through modulating its proline hydroxylation to mediate cell death and inflammation in human diseases, independent of TNFR1. (nature.com)
  • Creative Biolabs developed and successfully established a stable cell line, which can stably overexpress the Human CD16B protein. (creative-biolabs.com)
  • A broad range of viruses is susceptible to affecting human cell lines. (creative-biolabs.com)
  • Acquired immunodeficiency syndrome (AIDS) results from infection of cells with a virus called human immunodeficiency virus (HIV). (osri.asia)
  • 2012) Identification, isolation and invitro expansion of human and nonhuman primate T stem cell memory cells. (nexcelom.com)
  • His Cell biology research is multidisciplinary, incorporating perspectives in Jurkat cells and Human cytomegalovirus. (research.com)
  • included the precursors and derivatives of HSPCs, whereas the D20LS human population was heterogeneous and contained non\hematopoietic and differentiated cells. (bioxorio.com)
  • Herein we report that DCPA attenuates the normal elevated and sustained [Ca2+](i) that follows internal store depletion in the human leukemic Jurkat T cell line and primary mouse T cells. (cdc.gov)
  • This technology will enable researchers to greatly accelerate their studies in primary T cells and opens the possibilities for building increasingly complex screening paradigms applicable to human disease. (horizondiscovery.com)
  • Apoptotic effect of Nigella sativa on human lymphoma U937 cells. (istanbul.edu.tr)
  • Selective cytotoxicity of marine al-gae extracts to several human leukemic cell lines. (istanbul.edu.tr)
  • The complete cDNA sequence was determined by anchored PCR cloning and sequencing the entire coding sequence, using cDNA derived from a human leukemic T cell line (Jurkat). (bgu.ac.il)
  • In contrast, the expression levels in the brain and testes are considerably lower, and no transcripts were detected in several human carcinoma cell lines. (bgu.ac.il)
  • 1998. Protection of human upper respiratory tract cell lines against sulphur mustard toxicity by hexamethylenetetramine (HMT). (cdc.gov)
  • These positive control reagents, not provided with any other low cell ChIP kit on the market, save users the frustration of optimizing and troubleshooting protocols with samples they care about. (activemotif.com)
  • This protein is found to be a critical component of the lymphotoxin-beta receptor (LTbetaR) signaling complex, which induces NF-kappaB activation and cell death initiated by LTbeta ligation. (wikipedia.org)
  • Alvarez JD, Yasui DH, Niida H, Joh T, Loh DY, Kohwi-Shigematsu T. The MAR-binding protein SATB1 orchestrates temporal and spatial expression of multiple genes during T-cell development. (bdbiosciences.com)
  • I'm interested in using them in Jurkat cells after cloning the fusion protein into a retroviral vector, under the control of the retroviral promoter. (bio.net)
  • RNA from cells is then used for our Multiplex G-Protein profiling system. (gmu.edu)
  • In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. (bio-techne.com)
  • Secondly, we employed qRT-PCR and Western blotting to detect the mRNA and protein levels of GRHL2 in leukemia cell lines. (hindawi.com)
  • Then we investigated the demethylating effect of arsenic trioxide and 5-azacitidine on the mRNA and protein expression levels of GRHL2 in cell lines of acute leukemia. (hindawi.com)
  • The methylation level of the GRHL2 promoter region in acute leukemia patients and cell lines was significantly higher than the normal control group, and we found the elevated mRNA and protein levels of GRHL2 in acute leukemia cell lines after the use of the demethylation drug arsenic trioxide and 5-azacitidine. (hindawi.com)
  • Felipe Diaz-Griffero has researched Molecular biology in several fields, including Mutant, CDC2 Protein Kinase, Phosphorylation, RNA-binding protein and Binding site. (research.com)
  • The study incorporates disciplines such as Cytoplasm, Mutation, Sterile alpha motif, Wild type and Fusion protein in addition to Molecular biology. (research.com)
  • The scientist's investigation covers issues in Cell biology, Capsid, Cell cycle, Transmembrane protein and Phosphorylation. (research.com)
  • Milk fat globule CEGF-factor-8 and protein S are well known opsonins, stimulating phagocytosis of apoptotic cells (efferocytosis) acting as a bridge for the exposed PS [18], [19]. (cancerhugs.com)
  • Genome-wide CRISPR-Cas9 screening reveals ubiquitous T cell cancer targeting via the monomorphic MHC class I-related protein MR1. (bmj.com)
  • With Low Cell ChIP, researchers can reduce the number of cells they are studying to improve their understanding of the complexities of protein-DNA interactions to a more limited population of cells. (activemotif.com)
  • Members of the protein kinase C (PKC) family of serine/threonine kinases play a key role in regulating the differentiation and growth of diverse cell types and, to date, the cloning of seven mammalian PKC genes encoding eight distinct isoforms has been reported. (bgu.ac.il)
  • A rabbit antiserum raised against a unique (V3 domain) bacterially expressed PKCθ fragment immunoprecipitated specifically an 82- kDa protein from Jurkat cell lysates. (bgu.ac.il)
  • These include members of the Bcl-2 protein family, course, and extent of programmed cell death will aid man- which have both proapoptotic and antiapoptotic activity agement of patients with severe infections. (cdc.gov)
  • 2023 Jurkat-Lucia™ NFAT Cells for ADCC & ADCP Assays Hederman A.P. et al. (invivogen.com)
  • IgG2a also engages in high affinity interactions with Fc receptors on immune cells which can result in antibody-dependent cell-mediated cytotoxicity (ADCC) as well as antibody-dependent cellular phagocytosis (ADCP) by phagocytosing cells like macrophages 3-8 . (researchsquare.com)
  • Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues. (creative-biolabs.com)
  • EBV infects cells via the CR2 that was previously thought to be expressed only on the surface of B cells and certain epithelial cells. (nih.gov)
  • In addition, EBV variably infects Jurkat cells as demonstrated by the presence of transcripts of Epstein Barr nuclear Ag (EBNA-1) using the polymerase chain reaction. (nih.gov)
  • lysates were prepared from these and from a Jurkat T cell control. (tovi.io)
  • 1991) extended these findings by showing that cell exposure to H202 led the NF-ƙB activation. (osri.asia)
  • Anna Sannino, Olga Zeni, Stefania Romeo , Rita Massa, Maria Rosaria Scarfì, Adverse and beneficial effects in chinese hamster lung fibroblast cells following radiofrequency exposure, Bioelectromagnetics 38(4): 245-254, 2017. (cnr.it)
  • Exposure of Jurkat cells to either DCPA or 50 mu M 2-APB attenuated the increase in [Ca2+](i) following thapsigargin or anti-CD3 induced store depletion in a similar manner. (cdc.gov)
  • These changes are thought to lead to tumor-microenvironment interactions via factors secreted by stromal or epithelial cells in response to lymphoma cell-produced cytokines ( 7 ). (spandidos-publications.com)
  • Analysis of Jurkat and Raji mRNA by PCR demonstrated the presence of this novel splice variant in both cell lines. (nih.gov)
  • Methods: In the study, the chemical composition of Isochrysis galbana microalgae extract was analyzed, and its cytotoxic effect against K562, HL60, U937, MOLT-4, and Raji cancer cells was investigated. (istanbul.edu.tr)
  • Results: According to the results of the experiments, the highest cytotoxic effect of Isochrysis galbana microalgae extract was shown at about 24.07±6.48% cytotoxicity against Raji cells. (istanbul.edu.tr)
  • There were a large number of bioactive molecules in the ex tract, and these molecules showed a specific response to Raji cells when considering the synergistic and antagonistic effects of these molecules on each other. (istanbul.edu.tr)
  • The investigation of the effect of these molecules specifically against Raji cells is important to determine the possible anti-leukemic molecules and their combinations that show cytotoxicity against this cell line. (istanbul.edu.tr)
  • This size is consistent with that of B cell CR2 mRNA. (nih.gov)
  • Following substantial development, our screening platform uses freshly isolated and stimulated primary T cells which are first transduced with a lentiviral sgRNA library ahead of the introduction of Cas9 mRNA. (horizondiscovery.com)
  • 2023 ACE2 expressing HEK293 cells Bianchini F. et al. (invivogen.com)
  • Horizon scientists have recently successfully completed a pooled lentiviral CRISPR-Cas9 knockout screen in primary T cells. (horizondiscovery.com)
  • Isoenzyme analysis is used to confirm the identity of the species of a cell line. (creative-biolabs.com)
  • The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species. (creative-biolabs.com)
  • Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line. (creative-biolabs.com)
  • To our knowledge, there is only one commercially available T-cell line, CTLL-2. (takarabio.com)
  • To validate our platform, we monitored the rate of response of the T cells to a control set of sgRNAs and compared the performance of the primary T cell screen directly and side-by-side to a Jurkat cell line analysis. (horizondiscovery.com)
  • ECV304 endothelial cells were used as a healthy cell line for the understanding of its selective cytotoxicity. (istanbul.edu.tr)
  • This alteration in [Ca2+](i), when exposed to DCPA, significantly reduces nuclear levels of nuclear factor of activated T cells (NFAT) and IL-2 secretion. (cdc.gov)
  • Northern blot analysis of Jurkat poly(A)+ RNA shows a transcript of 4.7 kb upon probing with the B cell CR2 cDNA. (nih.gov)
  • Western blot analysis of Jurkat cell nuclear extract (40 µg) probed with ATAD2 antibody (AHP3026) at a dilution of 1/1000. (bio-rad-antibodies.com)
  • Gregory is a postdoc at the Lowy Cancer Research Centre, The University of New South Wales (UNSW) Kensington Campus, Sydney, Australia, investigating the regulation of endocytosis - how cells internalise what they need to function - and in particular how this ties in with serotonin and mental health. (biologists.com)
  • At 2 h intervals thereafter for the next 6 h the task was repeated with cells centrifuged to eliminate MPs that have been assayed by stream cytometry. (bioinf.org)
  • 2013) Accurate measurement of peripheral blood mononuclear cell concentration using image cytometry to eliminate RBC-induced counting error. (nexcelom.com)
  • RNase protection assays and semiquantitative PCR analysis indicated that, although PKCθ transcripts are expressed ubiquitously, the highest levels are found in hematopoietic tissues and cell lines, including T cells and thymocytes. (bgu.ac.il)
  • Interleukin 32 (IL32) is produced by T cells, NK cells and monocytes/macrophages, but also by a subset of melanoma cells. (biomedcentral.com)
  • PCI dosage decreased phagocytosis of apoptotic Jurkat cells by U937 macrophages dependently. (cancerhugs.com)
  • The outcomes of the scholarly research recommend a job of PCI not merely for the function and/or maturation of macrophages, but also as a poor regulator of apoptotic cell and turned on platelets removal. (cancerhugs.com)
  • Nuclear factor kappa B (NF-ƙB) is known to be a regulator of the activation of infected HIV leading for the virus to spread to other cells. (osri.asia)
  • After induction by stimuli such as treatment of cells with phorbol esters, the amount of cytoplasmic NF-ƙB-IKB complex is greatly reduced and the amount of nuclear NF-ƙB is increased. (osri.asia)
  • Nup153 Unlocks the Nuclear Pore Complex for HIV-1 Nuclear Translocation in Nondividing Cells. (research.com)
  • Two cDNA clones were identified upon screening of a Jurkat cell cDNA library with the B cell CR2 cDNA. (nih.gov)
  • Here we describe the molecular cloning and deduced primary structure of a cDNA encoding a novel PKC isoform, termed PKCθ, which was isolated in the course of attempts to identify PKC genes that are expressed selectively in hematopoietic cells. (bgu.ac.il)
  • Add 2 ml of the Jurkat cell suspension to a new sterile T75 flask containing 20 ml of complete growth medium and a final concentration of 1 mg/ml G418. (neb.com)
  • In this study, we demonstrate that the presence of serum in different media plays an important role in inducing transient and reversible adhesion in Jurkat suspension cells. (lsu.edu)
  • Recent findings in our laboratory and those of others, however, have shown that the EBV receptor is also present on T cells. (nih.gov)
  • Here we identify RIPK1, a known regulator of cell death mediated by tumour necrosis factor receptor 1 (TNFR1), as a target of EGLN1-pVHL. (nature.com)
  • T-cell receptor (TCR) diversity analysis using NGS has proven instrumental in probing for these questions, but no systematic study comparing TCR-seq methods was available until recently. (takarabio.com)
  • Why have there been no multiplex PCR methods evaluating the T-cell receptor α (TRA)? (takarabio.com)
  • TRA poses more challenges due to the increased number of genes involved, as compared to T-cell receptor β. (takarabio.com)
  • In " Benchmarking of T cell receptor repertoire profiling methods reveals large systematic biases ," we use standardized names to facilitate discussion. (takarabio.com)
  • Resistance to tetracycline arises from loss of cell wall permeability, tetracycline efflux, ribosome protection and Tetracycline modification. (toku-e.com)
  • Tetracycline is also used in multidrug resistance studies and in cell culture applications as a selective agent. (toku-e.com)
  • Tetracycline HCl is routinely used as a selective agent to select for bacterial cells that have been transformed with a plasmid that contains the tetracycline resistance gene, tet . (toku-e.com)
  • Genetic screens are an incredibly valuable tool to find and validate novel biology, drug targets and mechanisms of drug resistance or sensitivity. (horizondiscovery.com)
  • Next, we used methylation-specific polymerase chain reaction (MSP) technology for detecting the methylation of GRHL2 in clinical samples with acute leukemia and cell lines. (hindawi.com)
  • We found a lower level of GRHL2 expression not only in acute leukemia patients but also in cell lines when compared with normal controls. (hindawi.com)
  • Anti-adult T-cell leukemia effects of brown algae fucoxanthin and its deacetylated product, fucoxanthinol. (istanbul.edu.tr)
  • His Capsid research is multidisciplinary, relying on both Reverse transcriptase, Primary cell, Cypa, Cyclophilin A and Peripheral blood mononuclear cell. (research.com)
  • MR1-restricted TCRs with specificity for tumor cells have been described, raising interest in their potential therapeutic application for cancer treatment. (bmj.com)
  • We also evaluated the impact of proinflammatory molecules TNFα and IFNγ on IL32 expression and dedifferentiation in melanoma cell lines in vitro. (biomedcentral.com)
  • Tetracycline HCl) is a light-sensitive bacteriostatic polyketide antibiotic frequently used in a wide range of in vitro cell culture applications. (toku-e.com)
  • Mammalian cells are not vulnerable to the effect of Tetracycline as these cells contain no 30S ribosomal subunits so no accumulation results. (toku-e.com)
  • His research in SAMHD1 intersects with topics in Cell culture, Viral replication, Origin recognition complex and Kinase. (research.com)
  • The Jurkat E6.1 clone has been extensively used as a powerful tool for the genetic and biochemical dissection of the TCR signaling pathway. (ox.ac.uk)
  • Using DNA microarrays and Northern blots to analyze unstimulated cells, we demonstrate that expression of a cluster of genes, including RAG-1 and RAG-2, is repressed by constitutive signals requiring the adapter molecules LAT and SLP-76. (stanfordhealthcare.org)
  • Recently it has been reported that repression of SATB1 was essential for the phenotype and function of mouse regulatory T cells but inhibitory for the establishment of transcriptional programs expressed by effector T cells. (bdbiosciences.com)
  • In cells with constitutive NF-ƙB activity such as mature B lymphoblasts, NF-ƙB is isolated as a heterodimer of 50 and 65 kDa proteins (p50p65) which possesses DNA binding and transcriptional activation capabilities. (osri.asia)
  • Subsequent studies revealed that an inducible form of NF-ƙB is found in numerous cell types of both lymphoid and non-lymphoid origin, where it participates in activation of genes involved in the immune, inflammatory, or acute phase responses, such as various cytokines and surface receptors as well as viruses including HIV-1 (Osborn et al . (osri.asia)
  • New Therapeutic Procedure To Eliminate Cancer Cells Infiltrated In Brain. (usherbrooke.ca)
  • Functional activity of 7G5.TCRT was demonstrated by cytotoxicity utilizing the xCELLigence analyzer and by ELISA for cytokine release after co-culture with cancer cell lines with or without loading of known MR1 ligands. (bmj.com)
  • 1 Recognition is restricted to cells expressing a SNV of MR1 (MR1*04) and is not cancer specific. (bmj.com)
  • 7G5.TCRT and 7G5-like TCRT cells react to both cancer and healthy cells endogenously expressing MR1*04 SNVs, which encode R9H and H17R substitutions. (bmj.com)
  • Conclusions These data demonstrate that healthy individuals can harbor T cells reactive to MR1 variants displaying self-ligands expressed in cancer and benign tissues. (bmj.com)
  • The Podophyllotoxin cell-free supernatant was centrifuged to isolate MPs for assay further. (bioinf.org)
  • The comet assay is a technique which can evaluate the DNA integrity of cells. (scialert.net)
  • The assay is ideal if working with limited sample amounts, hard to culture cell lines, or small tissue biopsies. (activemotif.com)
  • Besides that the serpin is synthesized by many cell types and tissues and is present in almost every body fluid. (cancerhugs.com)
  • Melanoma cell lines were treated with 1000 IU/mL TNFα and 100 IU/mL IFNγ (PeproTech US, Rocky Hill, NJ) for the indicated time points. (biomedcentral.com)
  • Are both markers well expressed, in what cell lines, any toxicity. (bio.net)
  • 1992. Flow cytometric DNA analysis of nurse shark (Ginglymostoma cirratum) and clearnose skate (Raja eglanteria) peripheral red blood cells. (mote.org)
  • In our validation screen, we performed a drug-gene interaction analysis by exposing an edited pool of primary T cells to prolonged treatment with a sub-lethal dose of a specific small molecule inhibitor. (horizondiscovery.com)
  • We took multiple time points and used T cells isolated from multiple donors, allowing for the analysis of response kinetics and evaluation of donor-specific effects. (horizondiscovery.com)
  • They can act as signalling molecules that regulate a number of life and death pathways in cells, and they are generated by the immune system to fight microbes. (otago.ac.nz)
  • Active Motif's Low Cell Optimization Module enables Low Cell ChIP Kit users to monitor sample retainment as they progress through the protocol. (activemotif.com)
  • Using this module, researchers can monitor low cell samples throughout the ChIP protocol, identify and optimize steps responsible for sample loss, and save time and money by only moving forward with samples that will generate valuable ChIP-Seq data. (activemotif.com)
  • This module is intended to be used together with the Low Cell ChIP Kit, either to optimize protocol steps prior to using your precious samples, or to troubleshoot the point in the protocol responsible for sample loss. (activemotif.com)
  • Positive control samples (Jurkat cell pellets) and antibody ( AbFlex ® H3K9ac ) are included in the module for user optimization of the Low Cell ChIP protocol. (activemotif.com)
  • By comparison, Jurkat cells have been used only scantily for imaging on supported lipid bilayers (SLBs) incorporating laterally mobile TCR and integrin ligands, which allow to study synaptic rearrangements of surface molecules and the fine architecture of the mature IS, likely due to limitations in the assembly of immune synapses with well-defined architecture. (ox.ac.uk)
  • Treatment for FL includes successive lines of therapy that unfortunately result in progressively shorter periods of disease-free survival followed by the development of either chemo-refractory disease, large cell transformation or death from treatment-related toxicities ( 2 ). (spandidos-publications.com)
  • Non IL32-expressing differentiated melanoma cell lines exposed to TNFα or IFNγ can be induced to express the three predominant isoforms (α, β, γ) of IL32. (biomedcentral.com)
  • Identity testing is required for newly established cell lines. (creative-biolabs.com)
  • This allelic specificity can be overcome by expressing supraphysiologic levels of the wild type MR1 (MR1*01) in cell lines. (bmj.com)
  • In U937 cells the appearance of PCI aswell as the top binding of PCI elevated as time passes of phorbol ester treatment/macrophage differentiation. (cancerhugs.com)
  • Chemical inhibitor of nonapoptotic cell death with therapeutic potential for ischemic brain injury. (nature.com)
  • The advent of CRISPR technologies and the use of T cells in personalized medicine have greatly accelerated both the therapeutic value of and interest in modified T cells. (horizondiscovery.com)
  • Since the drug we treated the T cells with had a known mechanism of action, these hits directly supported the specificity, sensitivity and quality of our platform. (horizondiscovery.com)