Binds expression rna. Medical search. Frequent questions

When the interaction propensity score of a disordered region exceeds that of the full-length protein (points above the red line), disorder is considered to promote interaction with RNA molecules. (biomedcentral.com)
RNA molecules are frequently modified with a terminal 2',3'-cyclic phosphate group as a result of endonuclease cleavage, exonuclease trimming, or de novo synthesis. (rcsb.org)
Because alternative RNA splicing allows different types of mRNA molecules to be created from a single gene, it generates the diversity of protein function and structure that is essential to complex organisms. (genomicglossaries.com)
RNA molecules which hybridize to complementary sequences in either RNA or DNA altering the function of the latter. (genomicglossaries.com)
More specifically, antisense oligonucleotides that are useful as reagents for target validation , or as drugs, are engineered molecules that differ from natural RNA but that have a base sequence that is recognized as being complementary to a very specific mRNA sequence. (genomicglossaries.com)
MicroRNAs, or miRNAs, are small RNA molecules that do not encode proteins but perform a regulatory role in several intracellular processes. (eurekalert.org)
When the ARM binds to HIV RNA, the two molecules form a more stable structure together. (mit.edu)
MicroRNAs are tiny ribonucleic acid (RNA) molecules (~22 nucleotides long) that recently have been found to play important roles in regulating gene expression in eukaryotic organisms, including plants and animals. (sciencedaily.com)
Messenger RNA (mRNA) molecules are encoded by genes and are themselves templates for the proteins that carry the main metabolic functions in a cell. (sciencedaily.com)
MicroRNAs are ~22 nucleotide long RNA molecules that provide substrate specificity to a protein complex known as the RNA-induced silencing complex. (sciencedaily.com)
Within the complex, microRNAs are thought to bind to mRNA molecules containing a complementary stretch of RNA sequence. (sciencedaily.com)
Dr Dimitrov said nanostrings bind to RNA molecules for digital gene expression analysis. (biologynews.net)
Gene expression generally produces proteins, but there are also non-coding genes that produce RNA molecules (rRNA, tRNA). (lu.se)
It is not only the genes that code for proteins that are methylated, but also parts of the DNA that code for regulatory functions (RNA molecules), which can have a greater effect than genes. (lu.se)

These non-coding RNAs include microRNAs, small interfering RNAs (siRNA), as well as spliceosomal small nuclear RNAs (snRNA). (wikipedia.org)
For example, exosomes and nanovesicles secreted by living cells serve as intercellular couriers of mRNA, microRNAs (miRNAs), long noncoding RNAs (lncRNAs), and proteins and have thus garnered considerable interest [ 3 ]. (jcancer.org)
Research reported in The Plant Cell shows that microRNAs control the accumulation of transcription factor proteins that regulate the expression of genes in the auxin response pathway. (sciencedaily.com)
MicroRNAs (miRNAs) are an integral part of the post-transcriptional machinery of gene expression and have been implicated in the carcinogenic cascade. (cdc.gov)

Notably, a large number of stress-related elements present in the promoter regions of some ZmCAMTA genes, indicating a genetic basis of stress expression regulation of these genes. (frontiersin.org)
CAMTAs participate in gene expression regulation by binding to the cis -elements in the promoter regions of numerous target genes. (frontiersin.org)
Although RBPs have a crucial role in post-transcriptional regulation in gene expression, relatively few RBPs have been studied systematically.It has now become clear that RNA-RBP interactions play important roles in many biological processes among organisms. (wikipedia.org)
Overall, our data suggest that butyrate can reduce the expression of inflammatory genes not only by transcriptional regulation, but also by post-transcriptional regulation via inhibition of mRNA stabilizing proteins. (metu.edu.tr)
Only 60% of yeast and 73% of the human RBPs have functions assigned to RNA biology or structural motifs known to convey RNA binding, and many intensively studied proteins surprisingly emerge as RBPs (termed 'enigmRBPs'), including almost all glycolytic enzymes, pointing to emerging connections between gene regulation and metabolism. (nature.com)
RNA-binding proteins (RBPs) mediate pivotal cellular functions such as RNA transport, degradation or translation and represent key effectors of post-transcriptional gene regulation. (nature.com)
While we and other labs have demonstrated the isolation of mRNA subsets that are potentially co-regulated using RNA binding proteins as reporters of gene expression, methods have not been described that provide information about coordinated posttranscriptional regulation within specific types of cells during tumorigenesis and development. (biomedcentral.com)
This study finds that in Multiple Myeloma (MM), AATF/Che-1 (Che-1), an RNA-binding protein fundamental to transcription regulation, interacts with paraspeckles via the lncRNA NEAT1_2 (NEAT1) and directly localizes on R-loops. (uni-koeln.de)
This rethinking of how transcription factors work may lead to a better understanding of gene regulation, and may provide new targets for RNA-based therapeutics. (mit.edu)
Beyond being the product of gene expression, RNA can also influence the regulation of chromatin. (portlandpress.com)
in some cases, there are clear examples of direct RNA-mediated chromatin regulation mechanisms stemming from these interactions, while others have yet to be determined. (portlandpress.com)
Recent studies have highlighted examples of chromatin regulation via RNA matchmaking, a term we use broadly here to describe intermolecular base-pairing interactions between one RNA molecule and an RNA or DNA match. (portlandpress.com)
There are many AGO1-like proteins in animals and other eukaryotes as well, indicating that the RNA-induced silencing complex is of ancient evolutionary origin, and that microRNA-mediated regulation of gene expression is shared among many eukaryotes. (sciencedaily.com)
The proper regulation of RNA processing is critical for muscle development and the fine-tuning of contractile ability among muscle fiber-types. (uni-muenchen.de)
Aberrant regulation of their activity or expression results in a range of neurological phenotypes including learning deficits, epilepsy, synaptic growth defects and neurodegeneration in model organisms. (findaphd.com)
To obtain mechanistic insights into how phosphorylation impacts on alternative splicing regulation we will combine available structural information for molecular modelling of ELAV multimerization and RNA binding to instruct experimental validation. (findaphd.com)
It was further determined that up-regulation of ABHD11-AS1 expression plays an important role in chronic Cr(VI) exposure-induced cell malignant transformation and tumorigenesis, and the stemness of human lung cancer cells. (cdc.gov)
Although, the number of omic techniques is ever expanding, the most developed omics technologies are high throughput DNA sequencing, transcriptomics (focused on gene expression), epigenomics (focused on epigenetic regulation of gene expression), proteomics (focused on large sets of proteins, the proteome) and metabolomics (focused on large sets of metabolites, the metabolome). (who.int)

The results showed that nine ZmCAMTA genes showed highly diversified gene structures and tissue-specific expression patterns. (frontiersin.org)
Many ZmCAMTA genes displayed high expression levels in the roots. (frontiersin.org)
Quantitative real-time PCR was used to test the expression of ZmCAMTA genes under several abiotic stresses (drought, salt, and cold), various stress-related hormones [abscisic acid, auxin, salicylic acid (SA), and jasmonic acid] and biotic stress [rice black-streaked dwarf virus (RBSDV) infection]. (frontiersin.org)
Furthermore, the expression pattern of ZmCAMTA genes under RBSDV infection was analyzed to investigate their potential roles in responses of different maize cultivated varieties to RBSDV. (frontiersin.org)
The expression of most ZmCAMTA genes responded to both abiotic and biotic stresses. (frontiersin.org)
Butyrate is a SCFA that was reported to alter the mRNA stability of inflammatory genes by increasiing the expression of the RNA binding protein (RBP) Tristetraprolin (TTP). (metu.edu.tr)
Expression analysis of the genes encoding RBPs using publicly available RNA sequencing data for different developmental time points of the mosquito life cycle starting from the ovary and eggs up to the adults revealed stage-specific expression with several genes preferentially expressed in early embryonic stages and blood -fed female ovaries . (bvsalud.org)
Synthetic antisense RNAs are used to effect the functioning of specific genes for investigative or therapeutic purposes. (genomicglossaries.com)
Transcription factors help to regulate gene expression-turning genes on or off and dialing up or down their level of activity-often in partnership with the proteins that they bind. (mit.edu)
When transcription factors had their ARMs mutated so they couldn't bind RNA, those transcription factors were less effective in finding their target sites, remaining at those sites and regulating genes. (mit.edu)
Auxin influences development by affecting the expression of numerous genes that control the processes of cell division and cell expansion in specific plant tissues at specific stages during the plant life cycle - e.g. for leaves, roots, and floral organs to develop in the correct patterns and correct time sequence. (sciencedaily.com)
One of these targets is the transcription factor AUXIN RESPONSE FACTOR17 (ARF17), which is thought to repress the expression of a number of other genes involved in auxin responses. (sciencedaily.com)
Bortoluzzi S, Alessi FD, Romualdi C, Danieli GA: Differential expression of genes coding for ribosomal proteins in different human tissues. (karger.com)
Compared to bacteria that remained on earth, the space-traveling Salmonella had changed expression of 167 genes. (sciencedaily.com)
Both genes are highly conserved, and their proteins are thought to have 2 functions: (1) formation of a bridge or scaffold between the DNA-binding transcription factors and the RNA polymerase II complex and (2) serving as histone acetyltransferases that open the chromatin structure, a process essential for gene expression. (medscape.com)
With a kinetic modeling approach we ascribe function to the observed OCT4-SOX2-NANOG network by making plausible assumptions about the interactions between the transcription factors at the gene promoter binding sites and RNA polymerase (RNAP), at each of the three genes as well as the target genes. (lu.se)
The switch stabilizes the expression levels of the three genes, and through their regulatory roles on the downstream target genes, leads to a binary decision: When OCT4, SOX2 and NANOG are expressed and the switch is ON, the self-renewal genes are ON and the differentiation genes are OFF. (lu.se)
This will include co-expression network analyses to identify groups of genes that are expressed similarly and might belong to the same or linked gene regulatory networks. (lu.se)
The student will evaluate gene expression in relation to measures of genetic diversity, differentiation, and neutrality across the genome to evaluate whether the sequences or the flanking regions of genes with stage-specific expression demonstrate signs of selection. (lu.se)

RNA-binding proteins (often abbreviated as RBPs) are proteins that bind to the double or single stranded RNA in cells and participate in forming ribonucleoprotein complexes. (wikipedia.org)
RBPs contain various structural motifs, such as RNA recognition motif (RRM), dsRNA binding domain, zinc finger and others. (wikipedia.org)
However, since most mature RNA is exported from the nucleus relatively quickly, most RBPs in the nucleus exist as complexes of protein and pre-mRNA called heterogeneous ribonucleoprotein particles (hnRNPs). (wikipedia.org)
Eukaryotic cells express diverse RBPs with unique RNA-binding activity and protein-protein interaction. (wikipedia.org)
All RBPs bind RNA, however they do so with different RNA-sequence specificities and affinities, which allows the RBPs to be as diverse as their targets and functions. (wikipedia.org)
Other than core splicesome complex, RBPs also bind to the sites of Cis-acting RNA elements that influence exons inclusion or exclusion during splicing. (wikipedia.org)
These sites are referred to as exonic splicing enhancers (ESEs), exonic splicing silencers (ESSs), intronic splicing enhancers (ISEs) and intronic splicing silencers (ISSs) and depending on their location of binding, RBPs work as splicing silencers or enhancers. (wikipedia.org)
We have hypothesized that butyrate may regulate gene expression post-transcriptionally through global effects on the expression or cytoplasmic trans location of RBPs. (metu.edu.tr)
Using bioinformatics analyses of publicly available microarray data as well as colon cancer cell lines treated with sodium butyrate, we have observed that butyrate treatment led to a general reduction in expression of several (but not all) RBPs and inhibition in the cytosolic translocation of HuR, a well-known stabilizing RBP. (metu.edu.tr)
RNA-binding proteins (RBPs) exert a broad range of biological functions. (nature.com)
Recently developed unbiased high content techniques to identify RBPs in vivo yielded information on differences in cell type-specific expression and/or RNA-binding activity of RBPs in mammalian cells 2 , 3 . (nature.com)
RNA-binding proteins (RBPs) are the proteins that bind RNAs and regulate their functioning. (bvsalud.org)
RBPs in mosquitoes are gaining attention due to their ability to bind flaviviruses and regulate their replication and transmission . (bvsalud.org)
aegypti RRM-containing RBPs included serine / arginine -rich (SR) proteins , polyadenylate- binding proteins (PABP), heteronuclear ribonucleoproteins ( hnRNP ), small nuclear ribonucleoproteins ( snRNP ), splicing factors , eukaryotic initiation factors , transformers, and nucleolysins. (bvsalud.org)
Collaboration among the multitude of RNA-binding proteins (RBPs) is ubiquitous, yet our understanding of these key regulatory complexes has been limited to single RBPs. (elifesciences.org)
Our results illuminate the molecular mechanism of a regulatory switch controlling crucial gene expression programs, and provide a framework for understanding how the partnering of RBPs evokes changes in binding specificity that underlie regulatory network dynamics. (elifesciences.org)
RNA binding proteins (RBPs) regulate the diverse steps in RNA processing, including alternative splicing, which generates fiber-type specific isoforms of structural proteins that confer contractile sarcomeres with distinct biomechanical properties. (uni-muenchen.de)
We find that RBPs exhibit dynamic temporal and fiber-type specific expression patterns in mRNA-Seq data and display muscle-specific phenotypes. (uni-muenchen.de)

Long non-coding RNA H19 regulates FOXM1 expression by competitively binding endogenous miR-342-3p in gallbladder cancer. (biomedcentral.com)
GXD's primary emphasis is on endogenous gene expression during development. (jax.org)
Endogenous antisense RNAs function as regulators of gene expression by a variety of mechanisms. (genomicglossaries.com)
We detected high levels of BNT162b2 in Huh7 cells and changes in gene expression of long interspersed nuclear element-1 (LINE-1), which is an endogenous reverse transcriptase. (lu.se)
SNHG3 was identified as a competitive endogenous RNA molecule to promote the malignant progression of colorectal cancer [ 8 ]. (hindawi.com)
Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)

Using the Caco-2 cell line, which undergoes spontaneous re-differentiation when grown past confluency, we observed a loss of VCAM-1 (vascular cell adhesion molecule 1) mRNA expression, while ICAM-1 (intercellular cell adhesion m. (metu.edu.tr)
RNA-like oligonucleotides that are complementary to a portion of a target mRNA molecule. (genomicglossaries.com)
A complementary RNA sequence that binds to a naturally occurring (sense) mRNA molecule, thus blocking its translation. (genomicglossaries.com)
When a cell needs to make a particular protein, it first copies the instructions from the matching gene into a molecule known as a messenger RNA (or an mRNA for short). (elifesciences.org)
A gene, the basic unit of heredity, is a segment of DNA containing all the information necessary to synthesize a polypeptide (protein) or a functional RNA molecule. (msdmanuals.com)
A gene is a segment of DNA that provides the code to construct a protein or RNA molecule. (msdmanuals.com)

Relative IGFBP-2 expression was measured using quantitative real-time polymerase chain reaction in 113 epithelial ovarian cancers and 6 normal ovarian surface epithelial samples. (rti.org)
As nuclear RNA emerges from RNA polymerase, RNA transcripts are immediately covered with RNA-binding proteins that regulate every aspect of RNA metabolism and function including RNA biogenesis, maturation, transport, cellular localization and stability. (wikipedia.org)
87(5): 1663-1667] Specifically, the investigators started by priming whole cerebellar RNA with a synthetic oligonucleotide containing a T7 RNA polymerase promoter sequence. (genomicglossaries.com)
After second- strand cDNA synthesis, T7 RNA polymerase was used to generate aRNA. (genomicglossaries.com)
The majority of the human genome has the capacity to be transcribed and the majority of the non-protein-coding transcripts made by RNA Polymerase II are enriched in the nucleus. (portlandpress.com)

This process effectively changes the RNA sequence from that encoded by the genome and extends the diversity of the gene products. (wikipedia.org)
In this study, we screened the whole genome of Aedes aegypti , the primary vector of several pathogenic viruses , and identified the proteins containing RNA recognition motif (RRM), the most abundant protein domain in eukaryotes . (bvsalud.org)
Tat increases the transcription of HIV's RNA genome by binding to the virus' RNA and then recruiting cellular machinery to it. (mit.edu)
Overall, our analyses provide novel opportunities for the normalisation of genome-wide expression profiling data. (biorxiv.org)
Given that the basic genome of a differentiated organism is constant between cells (and we here ignore epigenomics), what mainly discriminates one cell type from another is its expression profile. (biorxiv.org)
Furthermore, a recent study showed that SARS-CoV-2 RNA can be reverse-transcribed and in-tegrated into the genome of human cells. (lu.se)
We have extensive whole genome and RNA sequencing data taken from whole samples of flies at three life stages: larva, pupa and adult. (lu.se)
We want to understand differences in life stage-specific gene expression in the peacock fly and relate these patterns to signatures of selection across the genome. (lu.se)

In a paper published in Molecular Cell on July 3 , Young and postdocs Ozgur Oksuz and Jonathan Henninger reveal that along with DNA and protein, many transcription factors can also bind RNA. (mit.edu)
The researchers found that RNA binding keeps transcription factors near their DNA binding sites for longer, helping to fine tune gene expression. (mit.edu)
A few papers, including one from Young's lab, had previously identified individual transcription factors as being able to bind RNA, but researchers thought that this was a quirk of the specific transcription factors. (mit.edu)
Instead, Young, Oksuz, Henninger and collaborators have shown that RNA-binding is in fact a common feature present in at least half of transcription factors. (mit.edu)
We show that RNA binding by transcription factors is a general phenomenon," Oksuz says. (mit.edu)
Other studies dismissed signs of RNA binding in transcription factors as an artifact-an accident of the experiment rather than a real finding. (mit.edu)
The reason that researchers had not recognized transcription factors' RNA binding region as such is because it is not a typical RNA binding domain. (mit.edu)
Transcription factors do not contain such structures, and so standard searches for RNA binding domains had not identified them in transcription factors. (mit.edu)
They went through the list of transcription factors, and instead of looking for structured RNA binding domains, they looked for ARMs. (mit.edu)
the majority of human transcription factors contain an ARM-like region between their DNA and protein binding regions, and these sequences were conserved across animal species. (mit.edu)
Further testing confirmed that many transcription factors do in fact use their ARMs to bind RNA. (mit.edu)
Next, the researchers tested to see if RNA binding affected the transcription factors' function. (mit.edu)
Transcription factors bind alongside the promoter and set off the gene expression. (lu.se)
Transcription factors are a group of proteins that are needed for a gene to start being copied to RNA, i.e. for transcription to start. (lu.se)
Certain transcription factors do not bind when the DNA is methylated while others only bind when the DNA is methylated. (lu.se)

Alternative splicing is a mechanism by which different forms of mature mRNAs (messengers RNAs) are generated from the same gene. (wikipedia.org)
Crucial to this process are a number of RNA-binding proteins, responsible for the posttranscriptional control of essential mRNAs and particularly pertinent to the two periods of inactive transcription that occur in spermatogenesis. (edu.au)
The selenocysteine insertion sequence (SECIS)-binding protein 2 (Secisbp2) binds to SECIS elements located in the 3'-untranslated region of eukaryotic selenoprotein mRNAs. (nih.gov)
Despite the massive reduction of Dio1 and Sepp1 mRNAs, significantly more corresponding protein was detected in primary hepatocytes lacking Secisbp2 than in cells lacking tRNA[Ser]Sec. Regarding selenoprotein expression, compensatory nuclear factor, erythroid-derived, like 2 (Nrf2)-dependent gene expression, or embryonic development, phenotypes were always milder in Secisbp2-deficient than in tRNA[Ser]Sec-deficient mice. (nih.gov)
Here we demonstrate that RNA-binding proteins can be used to isolate mRNA populations representing total cell mRNA from specific types of cells, as well as discrete mRNA subpopulations that represent post-transcriptionally regulated subsets of mRNAs that encode functionally related proteins. (biomedcentral.com)
For example, we identified subpopulations of mRNAs that are associated with ELAV/Hu RNA-binding proteins that are expressed in specific cell types [ 10 ]. (biomedcentral.com)
Moreover, because many different mRNA-binding proteins in specific cell types are known to interact with unique subpopulations of mRNAs encoding functionally related proteins [ 9 - 15 ] they can be informative of the dynamic effects of cells on one another. (biomedcentral.com)
In this study, we define a model system for using poly (A) binding protein (PABP) to recover mRNAs from specific cell-types in mixed cell cultures. (biomedcentral.com)
RNA-binding proteins contribute to specificity by interacting with both Ccr4-Not and target mRNAs, but this is not fully understood. (elifesciences.org)
Our results show how the specificity of one RBP (Pum) is modulated by cooperative RNA recognition with a second RBP (Nos) to synergistically repress mRNAs. (elifesciences.org)
Nos shifts the recognition sequence and promotes repression complex formation on mRNAs that are not stably bound by Pum alone, explaining the preponderance of sub-optimal Pum sites regulated in vivo . (elifesciences.org)
The expression profiles of long noncoding RNAs (lncRNAs) and mRNAs of PEX-treated dendritic cells are unknown. (jcancer.org)
To our knowledge, this is the first study to detect the differential expression of lncRNAs and mRNAs associated with PEX-treated DCs. (jcancer.org)
ELAV/Hu proteins are gene-specific regulators of alternative splicing of mRNAs, but can also affect other aspects of the maturation of an mRNA and its cytoplasmic expression into a protein. (findaphd.com)

Some binding proteins such as neuronal specific RNA-binding proteins, namely NOVA1, control the alternative splicing of a subset of hnRNA by recognizing and binding to a specific sequence in the RNA (YCAY where Y indicates pyrimidine, U or C). These proteins then recruit splicesomal proteins to this target site. (wikipedia.org)
however, some protein-encoding RNA transcripts have been shown to be subject to editing resulting in a difference in their protein's amino acid sequence. (wikipedia.org)
RNA which contains an intron sequence that has an enzyme- like catalytic activity. (genomicglossaries.com)
Using a two-color assay to simultaneously monitor poly(A) tail removal from different RNAs, we demonstrate that Puf3 can distinguish between RNAs of very similar sequence. (elifesciences.org)
Crystal structures of Nos-Pum-RNA complexes reveal that Nos embraces Pum and RNA, contributes sequence-specific contacts, and increases Pum RNA-binding affinity. (elifesciences.org)
Sequence-specific RNA-Binding Protein CPEB3 has distinctive isoform-distinct biochemical interactions and neuronal development assembly roles. (aging-us.com)
Transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. (novusbio.com)
Since ELAV/Hu proteins bind short U-rich motifs embedded in a highly degenerate sequence context, it is thought that multimerization of ELAV/Hu proteins is key to generate target specificity and that this step is highly regulated by cellular signaling. (findaphd.com)
ELAV mediated 3'-end processing of ewg transcripts is evolutionary conserved despite sequence degeneration of the ELAV binding site. (findaphd.com)
Today we use epigenetics to mean changes to gene expression that are not caused by changes in the actual DNA sequence. (lu.se)
Gene expression is the process of several steps through which the information in a gene's DNA sequence is transferred to the structures and functions of a cell. (lu.se)

They especially play a major role in post-transcriptional control of RNAs, such as: splicing, polyadenylation, mRNA stabilization, mRNA localization and translation. (wikipedia.org)
This protein functions through post-transcriptional modification of mRNA transcripts by changing the nucleotide content of the RNA. (wikipedia.org)

We measured changes in Gc messenger RNA (mRNA) synthesis and levels of secreted protein resulting from treatment of hepatocytes with cytokines and hormones known to influence synthesis of other proteins of hepatic origin. (nih.gov)
Pretreatment with 5FU increased the production of protoporphyrin IX (the active photosensitizer) and proapoptotic p53 expression and upregulated the enzymes in the heme synthesis pathway, compared with control lesions. (aacr.org)
As a result, we identified a third protein (Mlc) that is required for toxin synthesis by binding to the toxin's DNA. (usda.gov)
Integration host factor (IHF) binds to and represses the leukotoxin promoter but neither CRP nor IHF is responsible for the anaerobic induction of ltxA RNA synthesis. (usda.gov)

MiRNAs have complementary nucleotide sequences, so they can bind to messenger RNAs and break them down or prevent their translation into proteins. (eurekalert.org)

DEAD-box helicase DDX27 regulates 3' end formation of ribosomal 47S RNA and stably associates with the PeBoW-complex. (nih.gov)
Qu W , Jin H , Chen B , Liu J , Li R , Guo W , Tian H , . CPEB3 regulates neuron-specific alternative splicing and involves neurogenesis gene expression. (aging-us.com)
Long noncoding RNA ABHD11-AS1 interacts with SART3 and regulates CD44 RNA alternative splicing to promote lung carcinogenesis. (cdc.gov)
Together, these findings indicate that lncRNA ABHD11-AS1 interacts with SART3 and regulates CD44 RNA alternative splicing to promote cell malignant transformation and lung carcinogenesis. (cdc.gov)

During pre-transfer RNA (tRNA) and unconventional messenger RNA (mRNA) splicing, 2',3'-cyclic phosphates are substrates of the tRNA ligase complex, and their removal is critical for recycling of tRNAs upon ribosome stalling. (rcsb.org)

RNA binding proteins can control protein expression by regulating transport, localization, splicing, polyadenylation and turnover of mRNA transcripts. (umassmed.edu)

The clues have been there all along, but I think earlier work was so focused on the DNA and protein interactions that they didn't consider RNA. (mit.edu)
In addition to demonstrating different interactions between CRP and Mlc and their effects on periodontal toxin production, we also discovered how the IHF protein decreases toxin production by blocking Mlc binding to the toxin DNA. (usda.gov)

Analyses of the mitochondrial hydroxysteroid dehydrogenase (HSD17B10) uncover the RNA-binding specificity of an enigmRBP. (nature.com)
We also determined the RNA targets of an RNA-binding mitochondrial enzyme and show its specificity in RNA binding. (nature.com)

Perturbing ANGEL2 expression affected the efficiency of pre-tRNA processing, X-box-binding protein 1 ( XBP1 ) mRNA splicing during the unfolded protein response, and tRNA nucleotidyltransferase 1 (TRNT1)-mediated CCA addition onto tRNAs. (rcsb.org)

A transcriptional arrest leads to a strong change in localization and dynamics of YmcA, YlbF and YaaT, supporting their involvement in global RNA degradation. (uni-marburg.de)
Increasing the binding strength of NANOG to OCT4 and SOX2, or increasing its basal transcriptional rate, leads to an irreversible bistable switch: The switch remains ON even when the activating signal is removed. (lu.se)

miRNAs are small noncoding RNAs that have broad regulatory functions on gene expression. (lu.se)

To address the second, we investigated RNAs bound by the metabolic enzyme hydroxysteroid dehydrogenase 17-β 10 (HSD17B10). (nature.com)

Our results indicate that ANGEL2 is involved in RNA pathways that rely on the ligation or hydrolysis of 2',3'-cyclic phosphates. (rcsb.org)
Furthermore, chromatophore-localized biosynthetic pathways as well as multiprotein complexes include proteins of dual genetic origin, suggesting that mechanisms evolved that coordinate gene expression levels between chromatophore and nucleus. (frontiersin.org)

RNA-binding motif protein 3 (RBM3), involved in cell survival, has paradoxically been linked to both oncogenesis as well as an increased survival in several cancers, including urothelial carcinoma (UCA). (biomedcentral.com)

They anchor themselves and their partner proteins to DNA at binding sites in genetic regulatory sequences, bringing together the components that are needed to make gene expression happen. (mit.edu)
The CRISPR RNA consists of repeat sequences that are separated by short spacer sequences, which are derived from the invader (Figure 1). (uni-ulm.de)

NcRNAs almost always function as ribonucleoprotein complexes and not as naked RNAs. (wikipedia.org)
Characterization of the structure and dynamics of protein-RNA complexes is essential for understanding how they function at the molecular level: how these proteins specifically recognize their target RNAs, what are the structure-function relationships, and assess their biological role in regulating gene expression. (umassmed.edu)
Characterization of the structure and dynamics of protein-RNA complexes is essential for understanding how they function at the molecular level: how these proteins specifically recognize their target RNAs, what are the structure-function relationships, and to assess their biological role in regulating gene expression. (umassmed.edu)

Drosophila is thus a powerful model to gain disease-relevant insight into cellular and molecular phenotypes observed when expression levels of splicing factors, spliceosome components and splicing dynamics are altered. (uni-muenchen.de)
Previous work identified two proteins in the bacterial cell (IHF, CRP) that directly affect toxin level by either directly or indirectly binding to the cellular DNA code responsible for toxin production. (usda.gov)
The RNA is held within the granules until the genetic information it carries is translated to produce proteins or until cellular signals or environmental factors trigger the RNA to be degraded. (medlineplus.gov)

To improve our understanding of the biological and clinical significance of somatic mutations on RNA processing in cancer, the authors developed a new method, known as MIRA (mutation identification for RNA alterations). (aacr.org)

These targets include mRNA, which codes for proteins, as well as a number of functional non-coding RNAs. (wikipedia.org)
Here we demonstrate that the epitope-tagged RNA binding protein, PABP, expressed separately in tumor cells and endothelial cells can be used to discriminate their respective mRNA targets from mixtures of these cells without significant mRNA reassortment or exchange. (biomedcentral.com)
Consequently, we used iRIP-seq to identify CPEB-binding targets. (aging-us.com)

Diversity enabled eukaryotic cells to utilize RNA exons in various arrangements, giving rise to a unique RNP (ribonucleoprotein) for each RNA. (wikipedia.org)

We used integrated lncRNA and mRNA microarrays to determine the expression profiles of PEX-treated DCs and normal DCs derived from five healthy donors. (jcancer.org)
The function and possible mechanism of lncRNA Small Nucleolar RNA Host Gene 3 (SNHG3) in GC have not been fully studied. (hindawi.com)
Luciferase reporter gene analysis and RNA immunoprecipitation (RIP) analysis confirmed the interaction between lncRNA SNHG3, miR-326, and TWIST. (hindawi.com)
Long noncoding RNA (lncRNA) is more than 200 nucleotides in length without significant protein-coding potential [ 5 ]. (hindawi.com)
The goal of this study is to investigate the mechanism of Cr(VI)-induced lung carcinogenesis focusing on the role of the lncRNA ABHD11 antisense RNA 1 (tail to tail) (ABHD11-AS1). (cdc.gov)
It was found that the lncRNA ABHD11-AS1 expression levels are up-regulated in chronic Cr(VI) exposure-transformed human bronchial epithelial cells, chronically Cr(VI)-exposed mouse lung tissues, and human lung cancer cells as well. (cdc.gov)

The present study analyzes the putative prognostic role of RBM3 in a large cohort of patients with urothelial carcinoma (UCA) of the bladder by analyzing RBM3 immunohistochemical expression in various benign urothelial tissues, as well as in invasive and metastatic urothelial lesions. (biomedcentral.com)
Tumors and complex tissues consist of mixtures of communicating cells that differ significantly in their gene expression status. (biomedcentral.com)
RNA-binding proteins can be used as reporters to elucidate components of operational mRNA networks and operons involved in regulating cell-type specific gene expression in tissues and tumors. (biomedcentral.com)
We analyse two comprehensive transcriptome datasets from human tissues and human-derived cell lines in terms of the expression profiles of the SLC and ABC families of membrane transporters. (biorxiv.org)
The Gini index (coefficient) characterises inequalities of distributions, and is used in a novel way to describe the distribution of the expression of each transporter among the different tissues and cell lines. (biorxiv.org)
Similar trends hold true for the expression profiles of transporters in different cell lines, suggesting that cell lines exhibit largely similar transport behaviour to that of tissues. (biorxiv.org)
Hence, it means that human testis could also play a crucial role in human speciation if these two tissues exhibit similar gene expression patterns. (karger.com)
Determining the large scale anatomy of gene expression patterns of various human tissues could draw a more convincing conclusion, and better our understanding of the correlation/inter-correlation among different tissues. (karger.com)
Among the 17 tissues, the highest similarity in gene expression patterns was between human brain and testis, based on DDD and clustering analysis. (karger.com)
The gene expression varies between different types of cell and when these epigenetic changes are inherited by daughter cells through cell division in the womb, different tissues and organs are built. (lu.se)
There may be scope for future work comparing miRNA expression in different tissues between hybrids and parental species. (lu.se)

Overall this study provides further evidence for the unique functions that RNA-binding protein isoforms occupy within spermatogenesis, and introduces the potential manipulation of the Musashi family proteins to elucidate the mechanisms of posttranscriptional gene expression during germ cell development. (edu.au)
In order to understand how different cell types influence one another's gene expression, it will be necessary to monitor the mRNA profiles of each cell type independently and to dissect the mechanisms that regulate their gene expression outcomes. (biomedcentral.com)
Through these mechanisms, polyglutamine-binding protein 1 is thought to help control the way genetic information is used (gene expression) in neurons. (medlineplus.gov)

These findings imply that similar to the situation in mitochondria and plastids, also in P. chromatophora nuclear factors evolved that control metabolite exchange and gene expression in the chromatophore. (frontiersin.org)
Two critical factors during this dauntingly complex process appear to be the establishment of metabolic connectivity between the symbiotic partners, and the evolution of nuclear control over protein expression levels within the organelle. (frontiersin.org)
Nuclear localized USP15 interacts with pre-mRNA processing factor 19 (PRPF19) to increase CD44 RNA alternative splicing activating β-catenin and enhancing cancer stemness. (cdc.gov)

These mitochondrial responses are governed by general principles of regulatory biology exemplified by changes in gene expression, protein translation, protein complex formation, transmembrane transport, enzymatic activities and metabolite levels. (nature.com)

A biological process that is crucial for gene expression in most animal life, including humans. (genomicglossaries.com)

A prerequisite for rapid adaptations is efficient RNA turnover, with endonuclease RNase Y playing a crucial role in mRNA stability as well as in maturation. (uni-marburg.de)

Changes in gene expression mediate growth and development in all living organisms. (lu.se)

First, analyses of ltxA RNA expression from defined leukotoxin promoter mutations in the chromosome identify -69 to -35 as the key control region and indicate that an activator protein modulates leukotoxin transcription. (usda.gov)

The calmodulin-binding transcription activators (CAMTA) play critical roles in plant growth and responses to environmental stimuli. (frontiersin.org)
We show that this conserved RNA interactome harbours many proteins without previously assigned roles in RNA biology (enigmRBPs), including surprisingly many metabolic enzymes. (nature.com)

One such group of RNA-binding proteins is the Musashi family, specifically Musashi-1 (MSI1) and Musashi-2 (MSI2), which act as key translational regulators in various stem cell populations and have been linked with the induction of tumorigenesis. (edu.au)
These proteins show similar domain architectures as known organelle-targeted expression regulators of the octotrico peptide repeat type in algae and plants. (frontiersin.org)
Apparently these chromatophore-targeted proteins evolved convergently to plastid-targeted expression regulators and are likely involved in gene expression control in the chromatophore. (frontiersin.org)

A special issue of Non-Coding RNA (ISSN 2311-553X). (mdpi.com)

This analysis revealed previously unidentified alterations in untranslated regions, long noncoding RNAs, and introns that may guide the interpretation of noncoding variants in cancer genomes. (aacr.org)

Strains for which quantitative PCR results and second immunoglobulin-binding protein expression was analyzed by Western blot. (cdc.gov)
During the flight, astronaut Heidemarie M. Stefanyshyn-Piper activated growth of the bacteria in sealed hardware and 'fixed' the cultures after a day of growth to determine changes in gene and protein expression levels. (sciencedaily.com)
After the bacteria returned to Earth, the group performed the first global analysis of Salmonella to measure the effect of space flight on gene and protein expression and virulence. (sciencedaily.com)

RNA-binding ability and structural disorder. (biomedcentral.com)
Further, this project will apply statistical analysis of data and we willl use molecular modelling of available structural information to make predictions how phosphorylations impacts on ELAV multimerization and RNA binding. (findaphd.com)

instead, it binds RNA from a region called an arginine-rich motif (ARM) that is unstructured but has a high affinity for RNA. (mit.edu)

ELAV/Hu proteins comprise a family of highly conserved neuronal RNA binding proteins important for the development of the nervous system and for neuronal functions. (findaphd.com)

In the present study, we examined the differential expression of mammalian MSI1 and MSI2 during germ cell development in the mouse testis. (edu.au)
After cell lysis, polyadenylated RNAs were captured on oligo d(T) beads followed by stringent washes to remove non-crosslinked proteins. (nature.com)
However, the gene expression profile of a whole tumor corresponds to the combined profiles of the different cell types contained within it (e.g. endothelial cells, T-cells, cancer cells, stromal cells, etc. (biomedcentral.com)
Monitoring the dynamics of gene expression in each cell type of a tumor during angiogenesis will advance understanding of tumorigenesis as well as organogenesis, in general. (biomedcentral.com)
Furthermore, loss of IL22 reduced the number of lung-infiltrating inflammatory cells and decreased the expression of protumor inflammatory cytokines, while also increasing the antitumor Th1 and cytotoxic T-cell responses. (aacr.org)
Our results indicate a fast up-take of BNT162b2 into human liver cell line Huh7, leading to changes in LINE-1 expression and distribution. (lu.se)
The expressions of SNHG3, miR-326, and TWIST in GC9811-P GC cell lines were detected by RT-qPCR. (hindawi.com)
These granules allow the transport and storage of RNA within the cell. (medlineplus.gov)
When a microRNA is present in the cell, it binds to the target sites and downregulates GFP expression, while in cells that do not express the microRNA GFP, it is expressed. (lu.se)
In addition, we demonstrate that combined targeting of IL-6 signaling and WNT5A expression, using an anti-IL-6 antibody and a WNT5A antagonist peptide (Box5), respectively, more effectively reduces melanoma cell dissemination, as compared to each factor alone. (lu.se)
Moreover, we show that the RNA-binding protein human antigen R (HuR) drives melanoma cell motility, and targeting HuR with a specific HuR inhibitor, MS-444, significantly reduces melanoma cell migration. (lu.se)

Audic S, Claverie JM: The significance of digital gene expression profiles. (karger.com)

This quantitative data is superior to other gene expression systems such as microarrays, which rely on the analogue measurement of fluorescence and therefore are less accurate and have a limited range. (biologynews.net)
Huh7 cells were exposed to BNT162b2, and quantitative PCR was performed on RNA extracted from the cells. (lu.se)

A specific protein's recognition of a specific RNA has evolved through the rearrangement of these few basic domains. (wikipedia.org)
In silico identification, characterization, and expression analysis of RNA recognition motif (RRM) containing RNA-binding proteins in Aedes aegypti. (bvsalud.org)

In order to approach these questions, we have used RNA-binding proteins such as ELAV/Hu, poly (A) binding protein (PABP) and cap-binding protein (eIF-4E) as reporters of gene expression. (biomedcentral.com)

Strong selection pressures experienced during one stage of development may differ from those experienced in other stages, and with consequences for the underlying patterns of gene expression. (lu.se)

Anatomy19

Organisms20

Diseases1

Chemicals and Drugs110

Analytical, Diagnostic and Therapeutic Techniques and Equipment30

Phenomena and Processes83

Information Science4

Health Care1

Molecules14

MicroRNAs4

Regulation18

Genes19

RBPs18

Endogenous6

Molecule6

Polymerase5

Genome8

Transcription factors15

MRNAs14

Sequence11

Post-transcriptional2

Synthesis4

Complementary1

Regulates4

Messenger RNA1

Transcripts1

Interactions2

Specificity2

TRNA1

Transcriptional2

MiRNAs1

Enzyme1

Pathways2

Motif protein1

Sequences2

Complexes3

Cellular3

Mutation1

Targets3

Eukaryotic cells1

LncRNA6

Tissues11

Mechanisms3

Nuclear3

Mitochondrial1

Biological1

Stability1

Mediate1

Analyses1

Roles2

Regulators3

ISSN1

Introns1

Protein expression3

Structural2

Affinity1

Neuronal1

Cell11

Digital gene expression1

Quantitative2

Recognition2

ELAV1

Patterns of gene expression1