• Oxysterol-binding protein 2 is a protein that in humans is encoded by the OSBP2 gene. (wikipedia.org)
  • The protein encoded by this gene contains a pleckstrin homology (PH) domain and an oxysterol-binding region. (wikipedia.org)
  • Northern blot analysis documented that two transcription factor genes chosen for further study, c-myc promoter-binding protein (MBP-1) and X-box binding protein 1 (XBP-1), were up-regulated in U266 cells about 3-fold relative to the cell cycle-dependent β-actin gene 12 h after IL-6 treatment. (elsevierpure.com)
  • We provide a method to simultaneously screen a library of antibody fragments for binding affinity and cytoplasmic solubility by using the Escherichia coli twin-arginine translocation pathway, which has an inherent quality control mechanism for intracellular protein folding, to display the antibody fragments on the inner membrane. (jove.com)
  • Commonly used methods for the display and screening of recombinant antibody libraries do not incorporate intracellular protein folding quality control, and, thus, the antigen-binding capability and cytoplasmic folding and solubility of antibodies engineered using these methods often must be engineered separately. (jove.com)
  • They have the ability to modulate protein activity by binding to a target protein inside cells to prevent protein-protein interactions, disrupt protein-nucleic acid interactions, or prevent substrate access to enzymes 1-5 . (jove.com)
  • Squamosa-promoter binding protein (SBP)-box genes play a critical role in regulating flower and fruit development. (jipb.net)
  • Zhang B, Liu X, Zhao G, Mao X, Li A, Jing R (2014) Molecular characterization and expression analysis of Triticum aestivum squamosa‐promoter binding protein‐box genes involved in ear development. (jipb.net)
  • The protein sequences of 54 known R genes were used to identify homologs from peanut ESTs from public databases. (jipb.net)
  • SH3 domains constitute a family of protein-protein interaction modules that bind to peptides displaying an X-proline-X-X-proline (XPXXP) consensus. (hal.science)
  • Measured by its binding ability in a functional ELISA.Immobilized human ACE2 protein (His tag) at 2μg/mL (100μL/well) can bind 2019-nCoV Spike Protein (RBD, rabbitFc Tag) (40592-V31H) , the EC50 of 2019-nCoV Spike Protein (RBD, rabbitFc Tag) is 2.0-6.0ng/mL. (proteomecommons.org)
  • A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike Protein (RBD) (YP_009724390.1) (Arg319-Phe541) was expressed with the Fc region of rabbit IgG1 at the C-terminus. (proteomecommons.org)
  • Aided by state-of-the-art, high-throughput computational methods, the current study simulated and analyzed the dynamics of competitive histamine binding at the tick-host interface using the available crystal structures of both the tick salivary lipocalin histamine-binding protein from Rhipicephalus appendiculatus and the human histamine receptor 1. (biomedcentral.com)
  • The crystal structure of one of these tick salivary Ra-HBPs (Ra-HBP2) revealed a few structural deviations from the archetypal lipocalin protein family, that it sequesters two histamine molecules, and also causes an inhibition of guinea-pig ileum to contract by competing with H1R/H2R for histamine binding [ 6 ]. (biomedcentral.com)
  • The identification and characterization of geometrical and physical-chemical properties in protein vacant spaces aggregates important information for steering rational drug design and functional characterization of binding and catalytic sites. (cnpem.br)
  • Many of the protein-protein interactions (PPI) or intra protein interactions are mediated by interactions between domains and specific patterns of sequence. (cnpem.br)
  • To investigate the APP in vivo interactome in an unbiased manner, we generated mice that harbor a mouse prion protein promoter-driven cDNA encoding human APP-695 fused to a C-terminal affinity tag. (jneurosci.org)
  • Calumenin is expressed in skeletal muscle (at protein level). (betalifesci.com)
  • Rab40c is a SOCS box-containing protein which binds Cullin5 to form a ubiquitin E3 ligase complex (Rab40c/CRL5) to regulate protein ubiquitylation. (life-science-alliance.org)
  • Mechanistically, we found that Rab40c binds the protein phosphatase 6 (PP6) complex and ubiquitylates one of its subunits, ankyrin repeat domain 28 (ANKRD28), thus leading to its lysosomal degradation. (life-science-alliance.org)
  • Description: SARS-CoV-2 2019-nCoV Spike protein S1 subunit, receptor binding domain (RBD), also known as SARS-CoV-2 spike RBD, novel coronavirus spike RBD and nCoV spike RBD, GenBank Accession No. QHD43416.1, a.a. 319-541, with C-terminal Fc-tag, expressed in a CHO cell expression system. (stainingkit.com)
  • It is the major viral surface protein that coronavirus uses to bind to the human cell surface receptor. (stainingkit.com)
  • The protein modeling suggests that there is strong interaction between Spike protein receptor-binding domain and its host receptor angiotensin-converting enzyme 2 (ACE2), which regulate both the cross-species and human-to-human transmissions of COVID-19 (5). (stainingkit.com)
  • The recent study has shown that the SARS-CoV-2 spike protein binds ACE2 with higher affinity than SARS-CoV spike protein (6). (stainingkit.com)
  • The pQE-TriSystem vector contains CAG, T5, and p10 promoters that enable 6xHis-tagged protein expression in mammalian, E. coli , and baculovirus-infected insect cells, respectively (see figure pQE TriSystem. pQE TriSystem. "> pQE TriSystem ). (qiagen.com)
  • The pQE vectors (see table and figure pQE Vectors. Numbered elements listed in table. "> pQE Vectors ) enable placement of the 6xHis tag at either the N- or C-terminus of the recombinant protein. (qiagen.com)
  • Native or wild-type quaternary protein structure is usually born from a single translated protein sequence with one ordered conformation with downstream protein interactions. (medscape.com)
  • Sofia Elmroth and her research team primarily study microRNAs, which are molecules that influence protein levels in the cells of the body indirectly by binding to messenger RNA. (lu.se)
  • High-density oligonucleotide arrays were used to identify genes differentially expressed between high and low ERG expressers. (nih.gov)
  • High ERG expression was associated with upregulation of 112 expressed-sequenced tags and named genes, many of which are involved in cell proliferation, differentiation, and apoptosis. (nih.gov)
  • Of these, 29 were found to correspond to known genes, 22 matched expressed sequence tags in public databases and 21 showed no sequence similarity to any existing entries. (elsevierpure.com)
  • However, when applying this concept to genomes of multicellular organisms such as mammals, one has to deal with a large number of false positive predictions since many transcription factor genes are only expressed in specific tissues or cell types. (lu.se)
  • We developed TS-REX, a database/software system that supports the analysis of tissue and cell type-specific transcription factor-gene networks based on expressed sequence tag abundance of transcription factor-encoding genes in UniGene EST libraries. (lu.se)
  • The use of expression levels of transcription factor-encoding genes according to hierarchical anatomical classifications covering different tissues and cell types makes it possible to filter out irrelevant binding site predictions and to identify candidates of potential functional importance for further experimental testing. (lu.se)
  • Therefore comparative genomic analysis of T . equi was undertaken to: 1) identify genes contributing to immune evasion and persistence in equid hosts, 2) identify genes involved in PBMC infection biology and 3) define the phylogenetic position of T . equi relative to sequenced apicomplexan parasites. (biomedcentral.com)
  • Comparative genomic analysis of T . equi revealed the phylogenetic positioning relative to seven apicomplexan parasites using deduced amino acid sequences from 150 genes placed it as a sister taxon to Theileria spp . (biomedcentral.com)
  • In yeast a single complex of three proteins termed HAP2 HAP3 and HAP5 binds to this sequence and in mammals the three components of the equivalent complex (called variously NF-Y CBF or CP1) are also represented by single genes. (camregbase.org)
  • Here we report the presence of multiple genes for each of the components of the CCAAT-binding complex HAP2 35 from Arabidopsis. (camregbase.org)
  • Several hundred partial genomic DNA sequences as nucleotide-binding-site leucine-rich repeat (NBS-LRR) resistance genes ( R ) have been identified, but a small portion with expressed transcripts has been found. (jipb.net)
  • A total of 1,053 ESTs corresponding to six different classes of known R genes were recovered, and assembled 156 contigs and 229 singletons as peanut-expressed RGAs. (jipb.net)
  • These expressed RGAs are the source for RGA-tagged marker development and identification of peanut resistance genes. (jipb.net)
  • Screening of the Expressed Sequence Tags database allowed the identification of three Eps8-related genes, whose SH3s also display unusual binding preferences and constitute a phylogenetically distinct subfamily within the SH3 family. (hal.science)
  • 6% of the ESTs matched neither GenBank cDNA sequences nor the newly assembled chicken genomic DNA sequence, three ESTs aligned with sequences designated to be on the Z_random, while one matched a W chromosome sequence and could be useful in cataloguing functionally important genes on this sex chromosome. (westminster.ac.uk)
  • Additionally, single nucleotide polymorphisms (SNPs) were identified and validated in 10 ESTs that showed 98% or higher sequence similarity to known chicken genes. (westminster.ac.uk)
  • Here, we have described resources that may be useful in comparative and functional genomic analysis of genes expressed in an important organ, the pineal gland, in a model and agriculturally important organism. (westminster.ac.uk)
  • Epi-", a Greek term meaning "above" or "on top of," in the term epigenetics , refers to modifications that do not change the DNA sequence itself but can turn genes on or off, affecting how transcription factors read genes. (prohealth.com)
  • Methylation generally serves to suppress gene transcription, meaning that genes with methyl tags are less likely to be expressed. (prohealth.com)
  • This is evident at the subfamily level comparisons since Ciona GPCR sequences are significantly analogous to vertebrate GPCR subfamilies even while exhibiting Ciona specific genes. (biomedcentral.com)
  • These sequences will be subjected to further researches on hardiness genes and their molecular mechanisms. (geneticsmr.com)
  • In the present study, we report the characterization of the full coding sequence of rainbow trout PGMRC1 and mPR beta cDNAs, their tissue distribution, their ovarian expression profiles during oogenesis, their hormonal regulation in the full grown ovary and the in situ localization of PGMRC1 mRNA in the ovary. (biomedcentral.com)
  • Here, we describe the isolation and characterization of expressed sequence tags (ESTs) isolated from a chicken pineal gland cDNA library. (westminster.ac.uk)
  • The prediction of transcription factor binding sites in genomic sequences is in principle very useful to identify upstream regulatory factors. (lu.se)
  • Additional homologs (two of AtHAP3 and one of AtHAP5) have been identified from available Arabidopsis genomic sequences. (camregbase.org)
  • HA proteins bind to sialoside receptors on the host cell surface. (cdc.gov)
  • Although a recent study ( 22 ) reported enhanced avidity of H5N6 viruses for human-type receptors, recombinant clade 2.3.4.4 highly pathogenic influenza A virus H5 proteins from virus isolates in North America show a strict avian receptor-binding preference ( 23 ). (cdc.gov)
  • In lower vertebrates, steroid-induced oocyte maturation is considered to involve membrane-bound progestin receptors. (biomedcentral.com)
  • Two totally distinct classes of putative membrane-bound progestin receptors have been reported in vertebrates. (biomedcentral.com)
  • The recent cloning and sequencing of a gene coding for a membranous progesterone receptor [ 5 ] in African clawed frog ( Xenopus laevis ) brought evidence for the existence of both intracellular and membrane progestin receptors [ 6 ]. (biomedcentral.com)
  • The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. (proteomecommons.org)
  • These conserved families of secreted morphogens/signals, such as Fibroblast Growth Factor (FGF), Hedgehog (Hh), Wingless (Wg)/Wnt, Epidermal Growth Factor (EGF), and Decapentaplegic (Dpp - a BMP homolog), act away from their sources and, upon binding to receptors, activate gene regulatory pathways to induce functions in recipient cells 1 , 2 . (biorxiv.org)
  • In response to tissue damage or a hypersensitivity reaction, histamine is released by mast cells and basophils that then bind to its native receptors (histamine receptors, H1R and/or H2R) to facilitate repairing agents or mediators of the immune response arriving at the injury site [ 1 ]. (biomedcentral.com)
  • Siglecs (sialic acid-binding immunoglobulin-like lectins) are single-pass cell surface receptors that have inhibitory activities on immune cells. (mdpi.com)
  • The IL-17C preparations from R&D Systems have been found to bind immobilized recombinant mouse IL-17B R/Fc in a functional ELISA. (rndsystems.com)
  • Here, we describe a protocol to screen a recombinant library of single-chain variable fragment (scFv) antibodies for antigen-binding and proper cytoplasmic folding simultaneously. (jove.com)
  • QIA express pQE vectors combine a powerful phage T5 promoter (recognized by E. coli RNA polymerase) with a double lac operator repression module to provide tightly regulated, high-level expression of recombinant proteins in E. coli . (qiagen.com)
  • We aimed to identify resistance gene analogs (RGAs) from peanut expressed sequence tags (ESTs) and to develop polymorphic markers. (jipb.net)
  • The leaves were harvested at various times after cold stress for total RNA extraction, which was used to generate expressed sequence tags (ESTs). (geneticsmr.com)
  • After randomly choosing, cloning and sequencing, 227 ESTs with high quality were obtained and submitted to GenBank database. (geneticsmr.com)
  • Using BLASTX, 79.7% of the ESTs shared homology with known functional DNA sequences and 20.3% shared significant homology with unknown proteins. (geneticsmr.com)
  • S1 mainly contains a receptor binding domain (RBD) , which is responsible for recognizing the cell surface receptor. (proteomecommons.org)
  • Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD) , leading to a higher mortality rate. (proteomecommons.org)
  • Antigen-binding and cytoplasmic solubility can be improved with subsequent rounds of mutagenesis and screening to engineer antibodies with high affinity and high cytoplasmic solubility for intracellular applications. (jove.com)
  • The proteins then fold under oxidizing conditions and are displayed on the cell surface or packaged into phage particles to screen for binding affinity 17,18 . (jove.com)
  • To assess the native APP interactome in brain, we generated mice expressing human APP-695 fused to a C-terminal affinity tag (APP-AT). (jneurosci.org)
  • The addition of an affinity tag allowed us to avoid the use of antibodies targeted toward the intracellular C-terminal tail of APP, a region to which a number of reported interacting proteins bind ( King and Turner, 2004 ). (jneurosci.org)
  • Calumenin also binds 7 calcium ions with a low affinity and may modulate calcium release from the sarcoplasmic reticulum. (betalifesci.com)
  • Knowledge of the evolution of receptor-binding specificity of these viruses, which might affect host range, is urgently needed. (cdc.gov)
  • We report that emergence of these viruses is accompanied by a change in receptor-binding specificity. (cdc.gov)
  • Altered receptor-binding specificity of virus clade 2.3.4.4 H5 proteins might have contributed to emergence and spread of H5Nx viruses. (cdc.gov)
  • To overcome the host plant defense response, M. phaseolina encodes a significant number of P450s, MFS type membrane transporters, glycosidases, transposases, and secondary metabolites in comparison to all sequenced ascomycete species. (biomedcentral.com)
  • Phylogenetic Analysis and Secondary Structure Prediction for Drought Tolerant Cap Binding Proteins in Plant Species. (cabgrid.res.in)
  • The diversity among GPCRs primarily stem from the presence of characteristic N-terminal extracellular domains and C-terminal intracellular domains and to a relatively lesser extent from the connecting loops which share limited sequence similarity. (biomedcentral.com)
  • 4 We screened the coding sequence of ESPN (GenBank accession number AL136880) by sequencing PCR amplified ESPN exons from genomic DNA of affected individuals in the two families. (bmj.com)
  • Three independent Arabidopsis HAP subunit 2 (AtHAP2) cDNAs were cloned by functional complementation of a yeast hap2 mutant and two independent forms each of AtHAP3 and AtHAP5 cDNAs were detected in the expressed sequence tag database. (camregbase.org)
  • You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. (betalifesci.com)
  • Analysis and Functional Annotation of Expressed Sequence Tags of Water Buffalo. (cabgrid.res.in)
  • It is unexpected that B. subtilis SQR without covalently bound FAD is not assembled in the E. coli membrane provided that all other aspects of assembly, including iron-sulfur cluster biogenesis, are functional in the heterologous system. (lu.se)
  • In contrast to ancestral clade 2.3.4 H5 proteins, novel clade 2.3.4.4 H5 proteins bind to fucosylated sialosides because of substitutions K222Q and S227R, which are unique for highly pathogenic influenza virus H5 proteins. (cdc.gov)
  • The Fp and Ip subunits are both very conserved with respect to composition and polypeptide sequence [1]. (lu.se)
  • [ 3 ] It is also important to understand that the same polypeptide sequence can produce many different patterns of interresidue or intraresidue interactions. (medscape.com)
  • We will be looking at working towards the $100,000 genome sequence landmark in 2007. (outsourcing-pharma.com)
  • Rare mutations in genome sequence that result from failed DNA repair are transmitted across generations through DNA replication during each cell division. (nature.com)
  • Unlike such genetic changes, epigenetic changes, which do not alter genome sequence, can result in three possible outcomes: passive dilution, active repair through negative feedback, or active maintenance through positive feedback. (nature.com)
  • The technology is based on 'sequencing-by-synthesis' technology, which begins by breaking up the DNA sample into fragments that are between 300 and 500 bases in length. (outsourcing-pharma.com)
  • These fragments are then ligated by two short adaptors, which provide primers for both amplification and sequencing of the fragment as well as a biotin tag that immobilises it onto a streptavidin-coated bead. (outsourcing-pharma.com)
  • Very dilute solutions containing the DNA fragments are used, such that each 28um bead binds only a single DNA fragment. (outsourcing-pharma.com)
  • In addition, we show that both mPR beta and PGMRC1, two members of distinct membrane-bound progestin receptor classes, exhibit highly similar ovarian expression profiles during the reproductive cycle with maximum levels during vitellogenesis and a down-expression during late vitellogenesis. (biomedcentral.com)
  • To define the molecular basis of the IL-6-induced mitogenic response in myeloma cells, we applied STAR (subtractive transcriptional amplification of mRNA), a new differential expression analysis technology, to isolate mRNAs preferentially expressed in IL-6-treated versus untreated cultures of the factor-responsive myeloma cell line U266. (elsevierpure.com)
  • Recovery is also observed in most cases when double-stranded RNA is used to silence the same coding sequence in different regulatory contexts that drive germline expression. (nature.com)
  • IL-17C has a very restricted expression pattern and was detected as a rare expressed sequence tag (EST) in an adult prostate and fetal kidney libraries (2). (rndsystems.com)
  • cBiTE expression and secretion was detected in supernatants from ICOVIR-15K-cBiTE-infected cells, and the secreted BiTEs bound specifically to both CD3 + and EGFR + cells. (aacrjournals.org)
  • Epigenetics, the study of changes in gene expression not caused by alterations in the DNA sequence, has revolutionized our understanding of genetics and its impact on health and longevity. (prohealth.com)
  • It does this by physically impeding the binding of transcription factors and other machinery necessary for gene expression. (prohealth.com)
  • The pQE TriSystem Vector allows for high-level expression of His-tagged proteins from a single vector containing three different expression systems. (qiagen.com)
  • Using this tag, we prepared mild detergent lysates from transgenic mouse brain cortical membrane preparations and isolated a number of previously identified APP-interacting proteins. (jneurosci.org)
  • Anti-Siglec-8 antibody administration in vivo to humanized and transgenic mice selectively expressing Siglec-8 on mouse eosinophils and mast cells confirmed the in vitro findings, and identified additional anti-inflammatory effects. (mdpi.com)
  • The flavin together with the iron-sulfur clusters function to mediate electron transfer from the dicarboxylate binding site on the Fp subunit to the membrane-anchor domain where quinone is reduced to quinol. (lu.se)
  • Flavinylation of Fp in B. subtilis does not require the Ip subunit or the membrane anchor but depends on folding of the Fp polypeptide into a state that can bind FAD and subsequent mediate covalent binding of the FAD [3, 4]. (lu.se)
  • In the Fp subunit of SQR, and the closely related membrane-associated fumarate reductase of anaerobic organisms, FAD is covalently attached via an 8α-N(3)-histidyl linkage to a histidine (His) in a conserved sequence in the N-terminal part of the polypeptide. (lu.se)
  • The system is capable of expressed sequence tag (EST) sequencing, the identification of small RNAs and transcription binding sites as well as whole genome sequencing and metagenomics. (outsourcing-pharma.com)
  • Liu Z, Feng S, Pandey MK, Chen X, Culbreath AK, Varshney RK, Guo B (2013) Identification of expressed resistance gene analogs from peanut ( Arachis hypogaea L.) expressed sequence tags. (jipb.net)
  • Twenty-eight simple sequence repeats (SSRs) were identified from 25 peanut expressed RGAs. (jipb.net)
  • Avian and human influenza A viruses prefer binding to sialic acids linked to a penultimate galactose by an α2-3 or α2-6 linkage, respectively ( 18 ). (cdc.gov)
  • Mouse and human IL-17C share 83% aa sequence identity. (rndsystems.com)
  • A DNA sequence encoding the human CALU (O43852-1) (Met1-Arg311) was expressed with a His tag at the C-terminus. (betalifesci.com)
  • Human IgG antibody Laboratories manufactures the spike rbd sequence reagents distributed by Genprice. (stainingkit.com)
  • The Spike Rbd Sequence reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (stainingkit.com)
  • Among these, Siglec-8 is a CD33-related family member selectively expressed on human mast cells and eosinophils, and at low levels on basophils. (mdpi.com)
  • By a combination of approaches including (i) screening of phage-displayed random peptide libraries, (ii) mapping of the binding regions on three physiological interactors of Eps8, (iii) alanine scanning of binding peptides and (iv) in vitro cross-linking, we demonstrate that a proline-X-X-aspartate-tyrosine (PXXDY) consensus is indispensable for binding to the SH3 domain of Eps8. (hal.science)
  • the gene in G5 ( TaSPL3 ) expressed constitutively. (jipb.net)
  • Following removal of the outer membrane, the scFvs displayed on the inner membrane are panned against a target antigen immobilized on magnetic beads to isolate scFvs that bind to the target antigen. (jove.com)
  • Analysis of genetic diversity and population structure of peanut cultivars and breeding lines from China, India and the US using simple sequence repeat markers [J]. J Integr Plant Biol. (jipb.net)
  • Johanna C. Winder, William Boulton, Asaf Salamov, Sarah Lena Eggers, Katja Metfies, Vincent Moulton, Thomas Mock (2023) Genetic and structural diversity of prokaryotic ice-binding proteins from the central Arctic Ocean. (mocklab.com)
  • Next-generation sequencing technologies have dramatically changed the approach to genetic diagnosis. (msdmanuals.com)
  • This revolutionary and rapidly evolving technology has moved a significant portion of the technical aspects of genetic diagnosis to next-generation sequencing and has become the mainstay of genetic diagnosis. (msdmanuals.com)
  • Sequencing of DNA gives a direct picture of our genetic make-up, while sequencing of the RNA that is naturally present in cells gives a picture of which parts of our non-coding DNA are used and in what way. (lu.se)
  • During the nucleotide flow each of the beads is sequenced in parallel, with the polymerase extending the sequencing strand only if the nucleotide is complimentary to the template strand. (outsourcing-pharma.com)
  • Compared to other lipid ATP-binding cassette transporters, ALDP has two substrate binding cavities formed by the transmembrane domains. (elifesciences.org)
  • If you sequence more than one nucleotide at once you get a stronger signal and we see a very linear response between the signal strength and the number of nucleotides added. (outsourcing-pharma.com)
  • The transmembrane domains (TMDs) and nucleotide-binding domains (NBDs) are indicated. (elifesciences.org)
  • Subsequent un-anchoring of FGFR-bound-FGF from the source cell membrane dissociates cytoneme contacts and delivers FGF target-specifically to ASP cytonemes for paracrine functions. (biorxiv.org)
  • It binds oxysterols such as 7-ketocholesterol and may inhibit their cytotoxicity. (wikipedia.org)
  • The Tat pathway ensures that only soluble, well-folded proteins are transported out of the cytoplasm and displayed on the inner membrane, thereby eliminating poorly folded scFvs prior to interrogation for antigen-binding. (jove.com)
  • SQR consists of a membrane-peripheral heterodimer domain (often called succinate dehydrogenase) which is tightly bound to a membrane-spanning anchor, which is a cytochrome with one or two heme groups depending on the organism. (lu.se)
  • With the full complement of prosthetic groups they are then bound to the heme-containing membrane-anchor to form the active SQR. (lu.se)
  • SQR in E. coli and B. subtilis can be assembled in the membrane without covalently bound FAD but are enzymatically inactive [3, 4, 6]. (lu.se)
  • Notably, certain B. subtilis mutants with mutated Fp lack covalently bound flavin and do not assemble the SQR polypeptides in the membrane [3]. (lu.se)
  • Notably, intratumoral injection of this cBiTE-expressing adenovirus increased the persistence and accumulation of tumor-infiltrating T cells in vivo , compared with the parental virus lacking such effects. (aacrjournals.org)
  • The B. subtilis Fp polypeptide was found to lack covalently bound FAD when produced in E. coli although E. coli Fp in the same cell was flavinylated [8]. (lu.se)
  • The slight difference in electrophoretic mobility of normal Fp and that produced in E. coli could be due to the absence of covalently FAD but could have another explanation because this difference was not observed for some mutant Fp without covalently bound FAD [3]. (lu.se)
  • From the resulting collection of STAR clones, sequence information was obtained for a total of 72 distinct transcripts. (elsevierpure.com)
  • Participated a training programme on Next Generation Sequencing: Data Analysis & Annotation during 12th - 16th March, 2013 at IISR, Calicut, India. (cabgrid.res.in)
  • The oncolytic adenovirus ICOVIR-15K was engineered to express an EGFR-targeting BiTE (cBiTE) antibody under the control of the major late promoter, leading to generation of ICOVIR-15K-cBiTE, which retained its oncolytic properties in vitro . (aacrjournals.org)
  • Here we report that a minimal combination of cis- regulatory sequences can support permanent RNA silencing of a single-copy transgene and its derivatives in C. elegans simply upon mating. (nature.com)
  • If these microRNAs are over or under expressed (increase or decrease in quantity) - for example owing to epigenetic changes to the DNA - this will have an impact on many other regulatory processes. (lu.se)
  • One of the things that is really important for high throughput DNA sequencing is for people to see that researchers can look at the whole genome of bacteria and viruses. (outsourcing-pharma.com)
  • This means that while our DNA sequence remains the same, how it's read and expressed can change based on these epigenetic modifications, significantly impacting our health and development. (prohealth.com)
  • The results are reported as relative luminescence units and as an index of relative specific autoimmune reactivity compared to non-specific/heterophilic IgG binding to albumin. (cdc.gov)
  • However, the sheer volume of information generated by sequencing the exome or genome results in a variety of interpretive problems that complicate understanding of the results. (msdmanuals.com)