Binds dna bacterial. Medical search. Frequent questions

In Neisseria gonorrhoeae , the primosome proteins that catalyze DNA replication restart differ from the well-studied primosome proteins of E. coli with respect to the number of proteins involved and the affinities of their physical interactions: the PriA:PriB interaction is weak in E. coli , but strong in N. gonorrhoeae , and the PriB:DNA interaction is strong in E. coli , but weak in N. gonorrhoeae . (biomedcentral.com)
Catalyzed by primosome proteins such as PriA, PriB, PriC, DnaT, and DnaG, DNA replication restart pathways facilitate origin-independent reloading of the replicative helicase onto a repaired DNA replication fork in a process that involves coordinated protein and nucleic acid binding within a nucleoprotein complex called the DNA replication restart primosome [ 2 ]. (biomedcentral.com)
PriA is thought to recognize and bind to repaired DNA replication forks and D-loop recombination intermediates, facilitate assembly of the primosome complex by recruiting other primosome proteins, and catalyze duplex DNA unwinding using energy furnished by hydrolysis of ATP [ 4 , 5 ]. (biomedcentral.com)
Here we describe an African swine fever virus (ASFV) protein encoded by the open reading frame 5-AR that shares structural and functional similarities with the family of bacterial histone-like proteins which include histone-like DNA binding proteins, integration host factor, and Bacillus phage SPO1 transcription factor, TF1. (illinois.edu)
Previous work identified two proteins in the bacterial cell (IHF, CRP) that directly affect toxin level by either directly or indirectly binding to the cellular DNA code responsible for toxin production. (usda.gov)
Our goals were to identify other proteins that affect bacterial toxin production and to elucidate how these proteins work together. (usda.gov)
Ribosomes are made mostly of rRNA (ribosomal ribonucleic acid), and bacterial cells contain more than 50 associated proteins, whereas plant and animal cells contain 80 associated proteins. (visiblebody.com)
This is done through regulation of the activity of proteins that already exist in the cell, but also by the binding of special proteins-transcription factors-to specific sites on the DNA, turning certain genes on or off, which in turn regulates the cell's production of various proteins. (sciencedaily.com)
Not least they indicate how various DNA-binding proteins affect each other by acting as 'roadblocks' that impede the process. (sciencedaily.com)
NDRs are functionally important in proteins throughout the three kingdoms of life as well as being implicated in cancer, amyloid diseases and bacterial pathogenesis. (nottingham.ac.uk)
At least four phylogenetically distinct groups of bacteria encode repeat proteins with the common ability to bind specific DNA sequences with a unique but conserved code. (uni-muenchen.de)
Repeat arrays of these proteins have been demonstrated or predicted to form almost identical tertiary structures: a right-handed super helix that wraps around the DNA double strand with the base specifying residue of each repeat positioned in the major groove next to its cognate target base. (uni-muenchen.de)
RipTALs are effector proteins delivered during bacterial wilt disease caused by R. solanacearum strains. (uni-muenchen.de)
In the case of the RipTALs this work should begin to unravel the role these proteins play in bacterial wilt disease, as a means to fight this devastating pathogen. (uni-muenchen.de)
In addition the TALEs and RipTALs may simply represent one face of the TALE-likes, a protein family mediating as yet unknown biological roles as bacterial DNA binding proteins. (uni-muenchen.de)
Sigma factors are a class of proteins constituting essential dissociable subunits that confer sequence-specific DNA binding properties to RNA polymerase (RNAP). (hindawi.com)
A bacterial toxin (yellow) is split into two pieces and each are guided to the spot to be edited on mitochondrial DNA by programmable DNA-binding proteins (orange and pink). (acs.org)
Mougous reached out to David R. Liu at the Broad Institute, Harvard University, and HHMI, because Liu's group had previously developed base editors , which are engineered proteins that make specific single-letter changes in DNA. (acs.org)
Quinupristin and Dalfopristin Quinupristin and dalfopristin are streptogramin antibiotics, which, like macrolides and lincosamides, inhibit the synthesis of bacterial proteins. (msdmanuals.com)

PriA belongs to the DExH family of DNA helicases and is well-conserved among sequenced bacterial genomes [ 3 ]. (biomedcentral.com)
We use bioinformatics analysis of bacterial (meta) genomes, classical biochemistry as well as hybrid methods in structural biology (x-ray crystallography, electron microscopy, and small-angle x-ray scattering) and microbiology (physiology and growth) in our work. (au.dk)
Homology-based reconstruction of regulatory networks for bacterial and archaeal genomes. (openwetware.org)
Characterization of promoters in archaeal genomes based on DNA structural parameters. (openwetware.org)
A comparison of the TF repertoire of S. aureus against 1209 sequenced bacterial genomes was carried out allowing us to identify a core set of orthologous TFs for the Staphylococacceae , and also allowing us to assign potential functions to previously uncharacterized TFs. (biomedcentral.com)
We have evaluated the orthologous distribution of these elements in other sequenced bacterial genomes using the repertoire of TFs identified in USA300, and identified a core set of regulators for both the Firmicutes phylum, and the Staphylococacceae group. (biomedcentral.com)

When electron microscopy was used, there was no evidence that virus particles bound directly to bacteria. (lu.se)
Centrifuge the bacterial cultures for 10 min at 1000 x g to pellet the bacteria. (integra-biosciences.com)
Up to now several compounds targeted against the ATP-binding site of bacteria gyrase have been known but couldn't be used as drugs due to their side effects on mammalian cells. (sciencedaily.com)
Bacteria expressing novobiocin-resistant (NovR) derivatives of gyrase or topo IV also exhibited DNA relaxation at acid pH, although further relaxation with novobiocin was not seen in the strain with NovRgyrase. (jic.ac.uk)
Thus, inhibition of the negative supercoiling activity of gyrase was the primary cause of enhanced DNA relaxation in drug-treated bacteria. (jic.ac.uk)
Biofilms form when groups of bacteria cover themselves in a sticky mixture of sugars, protein, and DNA. (the-scientist.com)
One research team at Chalmers, led by Fredrik Westerlund, contributes its expertise in the field of DNA-based detection of resistance to antibiotics in bacteria. (lu.se)

The study, to be published in a coming issue of Proceedings of the National Academy of Sciences (PNAS), compares the energy required to break off the interaction with DNA with the energy needed to glide along the DNA and how many times a protein binds back to the same DNA strand before it starts to look elsewhere. (sciencedaily.com)
Structural evidence that colicin A protein binds to a novel binding site of TolA protein in Escherichia coli periplasm Journal of Biological Chemistry. (nottingham.ac.uk)

A transcription regulator activity that modulates transcription of gene sets via selective and non-covalent binding to a specific double-stranded genomic DNA sequence (sometimes referred to as a motif) within a cis-regulatory region. (yeastgenome.org)
In this process, the modified alkaline lysis method and RNase treatment are used for creating cleared lysate with minimal genomic DNA and RNA contaminants. (primelabmed.com)
PIM5 binds and intercalates into bacterial genomic DNA upon entry of cells. (bvsalud.org)

In the first isolation step, the plasmid DNA is released from bacterial cells by alkaline lysis. (blirt.eu)
QIAfilter Cartridges , provided in QIAfilter, HiSpeed, and EndoFree Plasmid Kits, are special filter units designed to replace centrifugation following alkaline lysis of bacterial cells. (qiagen.com)
The alkaline lysis-based miniprep protocol typically yields 5-15 μg of plasmid DNA from 1.5ml overnight cultures. (integra-biosciences.com)

Quinolones inhibit two enzymes that are required for bacterial DNA synthesis, i.e. (cdc.gov)
As a result, we identified a third protein (Mlc) that is required for toxin synthesis by binding to the toxin's DNA. (usda.gov)
Integration host factor (IHF) binds to and represses the leukotoxin promoter but neither CRP nor IHF is responsible for the anaerobic induction of ltxA RNA synthesis. (usda.gov)
The increase in adherence of S. pneumoniae could be inhibited by the DNA synthesis inhibitor cytosine arabinofuranoside, which is known to block the late phase of the adenovirus infection. (lu.se)
In Escherichia coli, a DNA mismatch repair (MMR) pathway corrects errors that occur during DNA replication by coordinating the excision and re-synthesis of a long tract of the newly-replicated DNA between an epigenetic signal (a hemi-methylated d(GATC) site or a single-stranded nick) and the replication error after the error is identified by protein MutS. (duke.edu)
It binds to the 50S subunit of the ribosome, thus inhibiting bacterial protein synthesis. (msdmanuals.com)
All inhibit protein synthesis by binding to the 50S ribosomal subunit. (msdmanuals.com)

We showed that strains of Pseudomonas aeruginosa with increased mutation rates (caused by deletion of genes involved in DNA mismatch repair) produced more biofilm biomass in laboratory experiments. (southampton.ac.uk)
Scholars@Duke publication: A 'Semi-Protected Oligonucleotide Recombination' Assay for DNA Mismatch Repair in vivo Suggests Different Modes of Repair for Lagging Strand Mismatches. (duke.edu)
Mispair-bound human MutS-MutL complex triggers DNA incisions and activates mismatch repair. (cdc.gov)

DNA replication restart initiated by PriA helicase is a highly coordinated process and probably represents the major pathway of DNA replication restart in E. coli . (biomedcentral.com)
Lerner, R. A. & Brenner, S. DNA-encoded compound libraries as open source: a powerful pathway to new drugs. (nature.com)
This allows the coupling of ATP binding and hydrolysis to electron transfer against a redox potential gradient and metal incorporation to (re)activate one of the central players of the pathway. (degruyter.com)
Currently, her projects mainly focus on studying BRCA2 and Tel1 related protein-protein interactions involved in DNA damage response pathway, using Cryo-EM and other biophysics methods. (imperial.ac.uk)
Autophagy is a well-conserved lysosomal degradation pathway that plays key roles in bacterial infections. (frontiersin.org)

Crystal structure of the bacterial cell division regulator MinD. (degruyter.com)

They found out that green tea catechins inhibit essential bacterial enzyme DNA gyrase, which is the target of several existing clinically used drugs. (sciencedaily.com)
Comparison of methods used to inhibit bacterial activity in sulfide ore bioleaching studies. (cdc.gov)

In the other data set, mutants of a bacterial enzyme that controls resistance to penicillins and cephalosporins, TEM-1 beta-lactamase, were expressed in E. coli under the selective pressure of an antibiotic treatment. (frontiersin.org)
Within the RNAP holoenzyme, sigma factors provide promoter recognition specificity to the polymerase and contribute to DNA strand separation, then they dissociate from RNAP core enzyme and transcription initiation follows [ 1 ]. (hindawi.com)

The EXTRACTME PLASMID DNA 96-WELL KIT with filter plates is designed for high-throughput and efficient purification of high quality plasmid DNA from recombinant Escherichia coli strains. (blirt.eu)
The virus-infected cells were harvested at various times after infection and analyzed for the ability to bind strains of Haemophilus influenzae and Streptococcus pneumoniae. (lu.se)
Adenovirus (types 1, 2, 3, and 5) commonly causing respiratory tract infections increased the binding of adherent S. pneumoniae strains to the cells. (lu.se)
Bacterial strains and plasmids are described in Table 1 . (hindawi.com)

Genes are transcriptional units, and include bacterial operons. (yeastgenome.org)
Mitochondrial DNA contains 37 genes, all of which are essential for normal mitochondrial function. (medlineplus.gov)
The remaining genes provide instructions for making molecules called transfer RNA (tRNA) and ribosomal RNA (rRNA), which are chemical cousins of DNA. (medlineplus.gov)
The bacterial activator protein NorR binds to enhancer-like elements, upstream of the promoter site, and activates 54-dependent transcription of genes that encode nitric oxide detoxifying enzymes (NorVW), in response to NO stress. (strath.ac.uk)
DNA in intracellular Salmonella enterica serovar Typhimurium relaxes during growth in the acidified (pH 4-5) macrophage vacuole and DNA relaxation correlates with the upregulation of Salmonella genes involved in adaptation to the macrophage environment. (jic.ac.uk)
The eukaryotic transactivation domain, which lends this group their name, allows them to activate specifically targeted host genes for the benefit of the bacterial invader. (uni-muenchen.de)
In this context, DNA-binding transcription factors (TFs) play an important regulatory role by either repressing or activating genes in response to environmental and physiological conditions. (biomedcentral.com)

Bacterial biofilms play an important role in UTIs, responsible for persistent infections leading to recurrences and relapses. (mdpi.com)
Biofilms are increasingly recognised as the predominant mode of bacterial growth including within medical, engineered, and environmental contexts. (southampton.ac.uk)
This can contribute to biofilm dispersal, and provides a mechanism for the release of extracellular DNA (eDNA) within biofilms. (southampton.ac.uk)
We discovered that exogenous nitric oxide can act as a signal for the dispersal of bacterial biofilms. (southampton.ac.uk)

The purified plasmid DNA is eluted using a low ionic strength buffer (Elution Buffer) or water (pH 7.0-9.0) and may be used directly in all downstream applications such as PCR, qPCR, Southern blotting, DNA sequencing, enzymatic restriction, ligation and so forth, or stored until ready to use. (blirt.eu)
The quality of each production batch (LOT) of the EXTRACTME PLASMID DNA 96-WELL KIT is tested using standard QC procedures. (blirt.eu)
Yields of up to 10 mg (giga), 2.5 mg (mega), 500 µg (maxi), and 100 µg (midi) transfection-grade, high-copy plasmid DNA are achieved from culture (culture volumes depend on plasmid copy number, size of insert, host strain, and culture medium). (qiagen.com)
The cleared lysate is then loaded onto the anion-exchange tip where plasmid DNA selectively binds under appropriate low-salt and pH conditions. (qiagen.com)
The use of silica membrane-based DNA purification kits is a convenient way to prepare plasmid DNA samples for cloning or subsequent analysis, e.g. sequencing or restriction analysis. (integra-biosciences.com)
MACHEREY-NAGEL's NucleoSpin 96 Plasmid kit is designed for high throughput purification of high-copy plasmid DNA from E. coli in a 96 well plate format. (integra-biosciences.com)
Automation of the protocol on an ASSIST PLUS pipetting robot with a VIAFLO 12 channel 1250 μl electronic pipette offers an easy and efficient way to extract and purify plasmid DNA with minimal hands-on time, guaranteeing perfect and reproducible liquid handling while protecting the user from repetitive strain injuries. (integra-biosciences.com)
MACHEREY-NAGEL's NucleoSpin 96 Plasmid kit and NucleoVac 96 Vacuum Manifold are a proven method for high throughput plasmid DNA purification. (integra-biosciences.com)
The ASSIST PLUS pipetting robot is used to automate the pipetting steps of the MACHEREY-NAGEL NucleoSpin 96 Plasmid kit protocol for plasmid DNA purification. (integra-biosciences.com)
More than 400 µg of plasmid DNA is quantified with a spectrophotometer. (primelabmed.com)
Using a gravity-flow procedure, the plasmid DNA in crude lysate has been bound to the column. (primelabmed.com)
The purified plasmid DNA is suitable for transfection, sequencing reactions, PCR, and in-vitro transcription. (primelabmed.com)

96 well Culture Plate containing the centrifuged bacterial cells, placed on the Teleshake microplate shaker in portrait orientation. (integra-biosciences.com)

DNA-binding transcription factors have a fundamental role in this process, because they regulate transcription positively or negatively as a consequence of environmental signals. (caister.com)
In this chapter we briefly describe some of the most recent findings on regulatory network evolution from the perspective of DNA-binding transcription factors. (caister.com)
2023. Editorial: Role of Transcription Factors and Sigma Factors in Bacterial Stress. (openwetware.org)
Comparative genomics of DNA-binding transcription factors in archaeal and bacterial organisms. (openwetware.org)
Roughly 40 years ago it was observed that these transcription factors find their binding sites on DNA much more quickly that free diffusion in three dimensions would allow. (sciencedaily.com)
Theoretical and empirical studies have shown that it is likely that the transcription factors bind to a chromosome wherever they encounter one and then glide along the DNA in search of their binding sites. (sciencedaily.com)

Various advantages regarding to specificity, simplicity at operation, rapid response, potential miniaturized platform, low power requirement, and relatively low cost of the analysis make DNA-based biosensors great candidates for detection of many targets, including methylxanthine-based drugs, such as theophylline (TP), used for treating respiratory and cardiovascular problems, such as chronic obstructive pulmonary disease. (databasefootball.com)
Each repeat binds a single DNA base, and specificity is determined by the amino acid residue at position 13 of each repeat. (uni-muenchen.de)
We have performed comprehensive analysis of homeodomain and Cys2His2 zinc finger specificities in D. melanogaster - (fruit fly) in collaboration with Michael Brodsky (UMMS-MCCB) & Gary Stormo (Wash. U). We have coupled this data with specificity data from artificial zinc fingers and homeodomains that we have selected using our bacterial one-hybrid system. (umassmed.edu)
Using this information we can build simple qualitative models of recognition that allow the design of homeodomains with novel DNA-binding specificity. (umassmed.edu)

The ParB protein forms DNA bridging interactions around parS to condense DNA and earmark the bacterial chromosome for segregation. (elifesciences.org)

The DNA purification procedure utilizes spin 96-minicolumns plates with membranes which efficiently and selectively bind nucleic acids. (blirt.eu)
The nucleus stores information in the form of DNA and controls the activities of the cell by selectively transcribing RNA, which exits via the nuclear pores. (visiblebody.com)

This finding is significant because N. gonorrhoeae PriB's single-stranded DNA binding activity is weak relative to that of E. coli PriB, and in E. coli , PriB's single-stranded DNA binding activity is important for PriB stimulation of PriA helicase. (biomedcentral.com)
Furthermore, a N. gonorrhoeae PriB variant defective for binding single-stranded DNA can stimulate PriA's helicase activity, suggesting that DNA binding by PriB might not be important for PriB stimulation of PriA helicase in N. gonorrhoeae . (biomedcentral.com)
Recruitment of PriB to a PriA:DNA complex stabilizes PriA on the DNA [ 6 ] and enhances its helicase activity through a mechanism that involves PriB's single-stranded DNA-binding activity [ 7 ]. (biomedcentral.com)
The function of DnaT is not well understood, but it has been proposed that DnaT binding leads to dissociation of single-stranded DNA (ssDNA) from PriB through a competition mechanism, possibly exposing the ssDNA on the lagging strand template for reloading the replicative helicase, which ultimately leads to fork reactivation [ 8 ]. (biomedcentral.com)
Cloned p11.6 bound both double and single stranded DNA-cellulose columns. (illinois.edu)
The formation of such complexes may be investigated by electrochemical DNA-based biosensors which consist of a biorecognition element (double- or single-stranded DNA) immobilized on the working electrode surface. (databasefootball.com)
Nowadays, not many reports on DNA-methylxanthines interactions have been published, so we decided to perform an electrochemical and spectrometric study of the interaction of TP with double-stranded (dsDNA) or single-stranded (ssDNA) salmon sperm DNA, guanosine (GMP), or adenosine monophosphates (AMP) using square wave and cyclic voltammetry. (databasefootball.com)

DNA structural and physical properties reveal peculiarities in promoter sequences of the bacterium Escherichia coli K-12. (openwetware.org)
Mitochondrial DNA has remained inaccessible to those methods because the guide RNA molecules used to target specific DNA sequences to be edited can't get into mitochondria. (acs.org)

Then the lysate is applied to the purification minicolumn membrane and the DNA is bound. (blirt.eu)
QIAfilter Plasmid Kits provide anion-exchange-based plasmid purification with bacterial lysate clearing by filtration. (qiagen.com)

Moreover, the free C-terminal domain can rapidly decondense ParB networks independently of its ability to bind DNA. (elifesciences.org)

Monospecific antiserum prepared to the 5-AR bacterial expression product specifically immunoprecipitated a protein of approximately 11.6kDa from ASFV infected swine macrophages at late times post-infection. (illinois.edu)
The increased attachment may be one mechanism by which viruses precondition the respiratory mucosa for bacterial infection. (lu.se)
Here's the gum disease secret: Despite being a bacterial infection, our own immune system causes the bone loss. (rdhmag.com)

The Maxi Plasmid Kits use pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures. (primelabmed.com)

The QIAfilter Plasmid Kits combines QIAfilter Cartridges for rapid clearing of bacterial lysates by filtration instead of centrifugation, with proven QIAGEN-tips containing anion-exchange resin for efficient plasmid purification. (qiagen.com)

Bacterial DNA replication restart pathways facilitate reinitiation of DNA replication following disruptive encounters of a replisome with DNA damage, thereby allowing complete and faithful duplication of the genome. (biomedcentral.com)
Consequently, DNA replication forks that stall or collapse due to encounters of the replisome with DNA damage must be reactivated to allow complete replication of the genome and ensure survival of the cell. (biomedcentral.com)
DNA replication restart pathways provide bacterial cells with a mechanism to reactivate replisomes that are disrupted in this manner [ 2 ]. (biomedcentral.com)
The targets of quinolone activity are the bacterial DNA gyrase and topoisomerase IV, enzymes essential for DNA replication and transcription. (cdc.gov)
Echinacea enhances the immune system, by aiding in T-cell replication, macrophage activity, antibody binding, and neutrophil migration. (rdhmag.com)

We explore diverse elements associated with the evolution of regulatory networks, such as gene duplication, where new interactions can emerge together with their upstream and downstream binding sites. (caister.com)
The latter might seem impossible, as an arbitrary transcription factor normally exists in just a handful of copies inside a bacterial cell, and one of them has to find a specific binding site on the DNA spiral, which contains some five million base pairs, in order to turn a gene on or off," says Erik Marklund, one of the lead authors of the new study. (sciencedaily.com)
Creating more precise CRISPR/Cas9 nucleases for gene therapy - We are utilzing our expertise in protein engineering and protein-DNA recognition to create hybrid CRISPR/Cas9 nucleases to achieve single-site cleavage precision within the human genome. (umassmed.edu)
Investigation of bacterial and fungal communities in indoor and outdoor air of elementary school classrooms by 16S rRNA gene and ITS region sequencing. (cdc.gov)

Streptococcus pneumoniae , a leading cause of bac- the detailed mechanism of competence in S. pneumoniae teremia, sinusitis, otitis media, bacterial meningitis, has gradually been clarified ( 10 ). (cdc.gov)

In cells, they are presumably anchored at parS sites by sequence-specific interactions but must also contain many interactions with non-specific DNA (nsDNA), as well as self-association interactions that bridge ParB protomers to form DNA loops. (elifesciences.org)
The research done in the Cravens lab is inspired by a desire to understand life at a fundamental level, primarily through biophysical studies of DNA and sequence-specific DNA targeting molecules. (gonzaga.edu)
Working together with others I was able to show that repeats from each group of TALE-likes mediate sequence specific DNA binding, revealing a conserved code in each case. (uni-muenchen.de)

Structural analysis of the C-terminal domain reveals a dimer with a lysine-rich surface that binds DNA non-specifically and is essential for DNA condensation in vitro. (elifesciences.org)
Investigation of a DNA-ligand interaction helps us to enlighten the structural alterations of DNA and the action mechanism of guest molecules, for instance, drugs. (databasefootball.com)
An efficient tool for investigating a target binding sites on the DNA double helix structure, sequence preference, DNA secondary structure, and the structural variations in DNA-drug complexes is an infrared spectroscopic study. (databasefootball.com)

To deeply investigate the DNA-TP interaction, a single strand of salmon sperm DNA prepared by the denaturation of dsDNA was used to more effectively study the formation of DNA-TP complex because of a better steric access for electron transfer between the electrode surface and nucleobases. (databasefootball.com)
ALKBH2 preferentially binds double strand DNA (dsDNA) (Duncan et al. (reactome.org)

An important concept in the evaluation of data regarding transfusion-transmitted bacterial infections (TTBIs) is the definition of a case. (medscape.com)
Here, we review the many functions of autophagy in bacterial infections with a focus on macrophages, the first line of host defenses, and the replicative niche of numerous pathogens. (frontiersin.org)
Viruses are thought to facilitate bacterial infections of the respiratory tract, but the mechanisms are poorly understood. (lu.se)
Adenovirus (types 1, 2, 3, and 5) commonly causing respiratory tract infections increased the binding of. (lu.se)

These 'networks' were inferred to be dynamic and poorly-ordered, consisting of several DNA loops between distally bound ParB molecules. (elifesciences.org)
Small inorganic and organic molecules can non-covalently interact with DNA via formation of DNA-guest complexes. (databasefootball.com)
The detector is plugged into the mobile phone, whose camera enlarges the image through a technique which, in very simple terms, takes a picture of fluorescent light from dyes bound to the DNA molecules. (lu.se)

Despite these recent experiments converging on DNA bridging models to explain the ParB spreading phenomenon, the mechanism underpinning this behaviour remains unresolved. (elifesciences.org)
Mechanism of activation and activity of bacterial RNase (VapBC, RelBE, HicAB) and kinase (HipBA) toxin-antitoxin systems. (au.dk)
The mechanism of DNA binding is conserved. (uni-muenchen.de)

Because Protein G has greater affinity than Protein A for most mammalian IgGs, it may be used for the purification of mammalian IgGs that do not bind well to Protein A. Protein G binds with significantly greater capacity than Protein A to several IgG subclasses such as human IgG3, mouse IgG1 and rat IgG2a. (thermofisher.com)
QIAfilter Cartridges completely remove SDS precipitates and clear bacterial lysates in a fraction of the time needed for centrifugation, reducing plasmid-purification time by up to 1 hour. (qiagen.com)

Bacterial Regulatory Networks (Edited by: Alain A.M. Filloux). (caister.com)

The Lund researchers' technology analyses the organisms' DNA and does not require a culture, which means that healthcare staff or aid workers receive the test result rapidly without the trouble and expense of sending the sample away for analysis. (lu.se)
DNA from the pathogenic organisms is captured and then spread out in extremely narrow micro- and then nano-channels. (lu.se)

We report that N. gonorrhoeae PriA's DNA binding and unwinding activities are similar to those of E. coli PriA, and N. gonorrhoeae PriA's helicase activity is stimulated by its cognate PriB, as it is in E. coli . (biomedcentral.com)
Binding of MsNrtR cognate DNA is finely mapped, which can be disrupted by an ADP-ribose intermediate. (elifesciences.org)

This genetic material is known as mitochondrial DNA or mtDNA. (medlineplus.gov)
In humans, mitochondrial DNA spans about 16,500 DNA building blocks (base pairs), representing a small fraction of the total DNA in cells. (medlineplus.gov)
The following chromosomal conditions are associated with changes in the structure or number of copies of mitochondrial dna. (medlineplus.gov)
Changes in mitochondrial DNA are among the best-studied genetic factors associated with age-related hearing loss. (medlineplus.gov)
As people age, mitochondrial DNA accumulates damaging mutations, including deletions and other changes. (medlineplus.gov)
Mitochondrial DNA is especially vulnerable because it has a limited ability to repair itself. (medlineplus.gov)
As a result, reactive oxygen species easily damage mitochondrial DNA, causing cells to malfunction and ultimately to die. (medlineplus.gov)
Cells that have high energy demands, such as those in the inner ear that are critical for hearing, are particularly sensitive to the effects of mitochondrial DNA damage. (medlineplus.gov)
Some cases of cyclic vomiting syndrome, particularly those that begin in childhood, may be related to changes in mitochondrial DNA. (medlineplus.gov)
Some of the genetic changes alter single DNA building blocks (nucleotides), whereas others rearrange larger segments of mitochondrial DNA. (medlineplus.gov)
A new type of base editor works on mitochondrial DNA (blue helix). (acs.org)
Being able to edit mitochondrial DNA is very important from both a basic biology and a disease perspective," says Vamsi K. Mootha, an expert on mitochondrial biology at Harvard Medical School and the Howard Hughes Medical Institute (HHMI), who was involved in the new study. (acs.org)

DNA gyrase and topoisomerase IV. (cdc.gov)
By the use of NMR spectroscopy, researchers from Slovenia have now pinpointed the ATP-binding site of DNA gyrase as target of EGCG, the most abundant catechin from the green tea extract. (sciencedaily.com)
Negative supercoiling of DNA by gyrase is inhibited in Salmonella enterica serovar Typhimurium during adaptation to acid stress. (jic.ac.uk)
Inhibiting ATP binding by DNA gyrase and topo IV with novobiocin enhanced the effect of low pH on DNA relaxation. (jic.ac.uk)
The Salmonella cytosol reaches pH 5-6 in response to an external pH of 4-5: the ATP-dependent DNA supercoiling activity of purified gyrase was progressively inhibited by lowering the pH in this range, as was the ATP-dependent DNA relaxation activity of topo IV. (jic.ac.uk)
We propose that DNA relaxation in Salmonella within macrophage is due to acid-mediated impairment of the negative supercoiling activity of gyrase. (jic.ac.uk)

Unique to the norVW promoter in Escherichia coli is the presence of three enhancer sites associated with a binding site for 54-RNA polymerase. (strath.ac.uk)

The Golgi body (Golgi apparatus, Golgi complex) is a membrane-bound organelle located in the cytoplasm of eukaryotic cells. (visiblebody.com)
Each Golgi body contains stacks of small, flattened, membrane-bound sacs called cisternae. (visiblebody.com)
Mitochondria are membrane-bound organelles located in the cytoplasm of eukaryotic cells. (visiblebody.com)
The nucleus is a large membrane-bound organelle that contains the genetic information of eukaryotic cells. (visiblebody.com)

The estimated residual risk of contamination of blood products with bacterial agents is 1 in 5,000 for platelets and 1 in 30,000 for red blood cells. (medscape.com)
The present study analyzed the effect of adenovirus on bacterial adherence to human respiratory tract epithelial cells. (lu.se)
PIM5 has negligible cytotoxicity on human cells at bacterial-killing concentrations, comparable to the commercial antibiotics polymyxin B and colistin. (bvsalud.org)
Using the SURVEYOR nuclease assay 13 , we assessed the ability of each Cas9-sgRNA complex to generate indels in human embryonic kidney (HEK) 293FT cells through the induction of DNA doublestranded breaks (DSBs) and subsequent nonhomologous end joining (NHEJ) DNA damage repair (Online Methods). (cdc.gov)

it inhibits RNA polymerase by binding to the DNA template-RNA polymerase complex. (msdmanuals.com)

He then moved to the Tokyo Institute of Technology in Japan as a postdoctoral researcher to investigate the mechanisms of recombinational DNA repair through biochemical and genetic approaches (2016-2021). (imperial.ac.uk)

One type of DNA has remained beyond the reach of precision genome editing techniques like CRISPR. (acs.org)

A ligand fixation into DNA structure can be performed by electrostatic binding between a positively charged guest and negatively charged DNA host by binding to the major and minor groove of DNA and by intercalation, meaning the insertion of the ligand molecule between DNA base pairs. (databasefootball.com)

In molecular biology, the LuxR-type DNA-binding HTH domain is a DNA-binding, helix-turn-helix (HTH) domain of about 65 amino acids. (wikipedia.org)
The HTH binds DNA in the major groove, where the N-terminal part of the recognition helix makes most of the DNA contacts. (wikipedia.org)
After TP binding, the absorption bands of DNA nucleobases strongly diminished, which confirmed the DNA-TP interaction as well as the changes in DNA double helix structure. (databasefootball.com)

We demonstrate that this is essentially due to the need for all three enhancers for maximal ATPase activity of NorR, energy from which is used to remodel the closed E54 complex and allow melting of the promoter DNA. (strath.ac.uk)

Together, it represents a first paradigm for the recruitment of acetylated NrtR to regulate bacterial central NAD + metabolism. (elifesciences.org)

Silver then breaks bacteria's cell wall and binds to the DNA, preventing cell-reproduction and inhibiting cell respiratory function which causes the bacterial cell to burst. (primroseschools.com)

Some rare metabolic diseases are caused by mutations in this type of DNA. (acs.org)
The camera in an ordinary mobile phone could be used as a microscope for early, easy and cheap detection of common parasitical and bacterial diseases. (lu.se)
The project also includes researchers from Glasgow and Karolinska institutet in Stockholm, who have the medical expertise required for the project on bacterial and parasitical diseases, respectively. (lu.se)

Although most DNA is packaged in chromosomes within the nucleus, mitochondria also have a small amount of their own DNA. (medlineplus.gov)

For the current protocol, the bacterial cultures are grown in tubes and transferred into a Culture Plate. (integra-biosciences.com)

We show here that while the central DNA binding domain is essential for anchoring at parS , this interaction is not required for DNA condensation. (elifesciences.org)
Thus, the weak interaction between N. gonorrhoeae PriB and DNA might be compensated for by the strong interaction between PriB and PriA, which could result in allosteric activation of PriA's ATPase activity. (biomedcentral.com)

Anatomy13

Organisms13

Diseases1

Chemicals and Drugs110

Analytical, Diagnostic and Therapeutic Techniques and Equipment32

Phenomena and Processes90

Information Science3

Health Care2

Proteins19

Genomes6

Bacteria7

Protein binds2

Genomic3

Alkaline lysis3

Synthesis7

Mismatch repair3

Pathway5

Regulator1

Inhibit2

Enzyme2

Strains4

Genes7

Biofilms4

PLASMID DNA12

Centrifuged bacterial cells1

Transcription factors6

Specificity4

Chromosome1

Selectively2

Single-stranded7

Sequences2

Lysate2

Ability to bind1

Infection3

Purify plasmid1

Anion-exchange1

Replication5

Gene4

Sinusitis1

Sequence-specific3

Structural3

Strand2

Infections4

Molecules3

Mechanism3

Purification2

Regulatory Networks1

Organisms2

Cognate2

Mitochondrial DNA12

Gyrase6

Coli1

Membrane-bound4

Cells4

Polymerase1

Genetic1

CRISPR1

Molecule1

Helix3

Promoter1

Regulate1

Respiratory1

Diseases3

Mitochondria1

Cultures1

Interaction2