• The tertiary structure of CBDN1 is strikingly similar to that of the bacterial 1,3-1,4-beta-glucanases, as well as other sugar-binding proteins with jelly-roll folds. (wikipedia.org)
  • Carbohydrate-binding module family 10 (CBM10) is found in two distinct sets of proteins with different functions. (wikipedia.org)
  • Agrin-like glycoproteins and proteins with sugar-binding domains are also predominant. (biomedcentral.com)
  • may not generate fusion-promoting S protein conformations protein receptors that bind S proteins at high affinity clearly do, as evidenced most extensively by studies with MHV. (globaltechbiz.com)
  • It has been validated with a distinct set of proteins and binding sites. (cnpem.br)
  • Partial sequences were also obtained for peptides from other HLA-DR alleles but none of these were attributable to known proteins. (ox.ac.uk)
  • Lectins are important adhesin proteins, which bind carbohydrate structures on host cell surface. (unl.edu)
  • Availability of genome sequences facilitated the identification of pneumococcal surface proteins bearing characteristic motifs such as choline-binding proteins (Cbp) and peptidoglycan binding (LPXTG) proteins. (biomedcentral.com)
  • We conclude that whether peptidoglycan binding proteins do not appear to be major adhesins, most of the choline-binding proteins interact with host proteins (elastin and C reactive proteins are the major Cbp partners). (biomedcentral.com)
  • An antibody is used to precipitate a protein along with bound proteins. (genomicglossaries.com)
  • In S. pneumoniae, there are two proteins that share responsibility for Zn2+ homeostasis, one of them is the Adhesin Competence Repressor (AdcR) and it binds to a specific double-stranded DNA binding domain (dsDNA). (bvsalud.org)
  • A similar His3Asp site found in calprotectin (S100A8/S100A9, calgranulin A/B), a closely related human S100 protein, can sequester divalent metal ions from the solute-binding proteins (SBPs) pneumococcal PsaA (pneumococcal surface protein A) and staphylococcal MntC (manganese transport protein C). Both SBPs are components of Mn(II) transporters and capture extracellular Mn(II) ions for subsequent delivery into the bacterial cytosol. (bvsalud.org)
  • CBMs are classified into numerous families, based on amino acid sequence similarity. (wikipedia.org)
  • Many putative CBMs have been identified by amino acid sequence alignments but only a few representatives have been shown experimentally to have a carbohydrate-binding function. (wikipedia.org)
  • The derived amino acid sequence of Ov-phy-1 encoded a peptide that was most similar to the two Caenorhabditis elegans prolyl 4-hydroxylase homologues and to the isoform II enzymes of vertebrates. (embl.de)
  • Production of advanced sleep occur at a carbohydrate-amino acid sequence has been well as well known as well documented. (lorenzopetrantoni.com)
  • Amino acid sequence alignment from CLUSTALW (DNASTAR, Madison, WI). (cazypedia.org)
  • The structural integrity of the N-terminus is sensitive to changes in amino acid sequence surrounding N2 and possibly dependent on the presence of the carbohydrate moiety itself at N15 ( Tam and Moritz, 2009 ). (jneurosci.org)
  • Studies of AMINO ACID SEQUENCE HOMOLOGY and NUCLEIC ACID SEQUENCE HOMOLOGY provide useful information about the genetic relatedness of genes, gene products, and species. (bvsalud.org)
  • Its extracellular region consists of two N-linked glycosylation sites (aa 73 and 159), a coil‑coil neck domain (aa 96‑136), a C‑type lectin-like domain (CTLD) of the type found in human DC‑SIGN and DC‑SIGN receptor (aa 165‑289), and a C-terminal Ca 2+ -dependent carbohydrate‑recognition domain (C-type CRD) (1). (rndsystems.com)
  • After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. (joplink.net)
  • Rat liver asialoglycoprotein receptor lacks a cleavable NH2-terminal signal sequence. (joplink.net)
  • These species were identified in the process of characterizing mannose 6-phosphate/insulin-like growth factor II (M-6-P/IGF-II) receptor binding to the surface of B lymphoblasts. (ox.ac.uk)
  • Peptides eluted from the Man-6-P/IGF-II receptor-binding fraction of immunoaffinity-purified MHC class II from the Swei cell line contained a sequence derived from the propiece of lysosomal acid lipase. (ox.ac.uk)
  • The carbohydrate specificity of diverse lectins to a large extent dictates bacteria tissue tropism by mediating specific attachment to unique host sites expressing the corresponding carbohydrate receptor. (unl.edu)
  • Although bee-venom PLA2 does not belong to the large homologous Class I/II family that encompasses most other well-studied PLA2s, there is segmental sequence similarity and conservation of many functional substructures. (rcsb.org)
  • For reference, the CBM58 of SusG (BT_3698) extends from residues 216-335.Red, orange and green colors at the top indicate positions of complete conservation, similarity, or more diverse sequence respectively. (cazypedia.org)
  • The degree of similarity between sequences. (bvsalud.org)
  • Binding of peptides to MHC class II Ag generates ligands for TCR of Th lymphocytes. (ox.ac.uk)
  • They are isolated via SELEX (Systematic Evolution of Ligands by Exponential Enrichment), an evolutionary process that involves iterative rounds of selection and amplification before sequencing and aptamer characterization. (genomicglossaries.com)
  • This disease is a defect in fucose metabolism (lack of fucosylation of the carbohydrate selectin ligands) that results in failure to express the ligand for E and P selectin, sialyl Lewis-X (CD15s) expressed on leukocytes and endothelial cells. (medscape.com)
  • Recent immunochemical studies have found that normal human sera (NHS) contain bactericidal anti-LOS antibodies that bind to the oligosaccharide (OS) moiety of neisserial LOS. (go.jp)
  • CD62L binds to carbohydrates present on certain glycoforms of CD34, glycam-1, and MAdCAM-1 and with a low affinity to anionic oligosaccharide sequences related to sialylated Lewis X (sLex, CD15s) through its C-type lectin domain. (biolegend.com)
  • Currently, 18 subtypes have been reported: 12 are type I (dysfunctional lipid-linked oligosaccharide precursor synthesis), and 6 are type II (dysfunctional trimming/processing of the protein-bound oligosaccharide), including leukocyte adhesion deficiency II (CDG-IIc). (medscape.com)
  • However, the three lectins also display differences in binding preferences. (unl.pt)
  • We were able to identify several classes of antimicrobial peptides, such as buforins, peroniins and brevinins, as well as PLA 2 , lectins and galectins, combining protein sequencing and RNA-seq analysis for the first time. (biomedcentral.com)
  • The detection level accomplished in this study should help not only to further characterize anti-LOS antibodies in blood and body fluids but also to analyze carbohydrate structures that are recognized by them. (go.jp)
  • Based upon these data, the CBM58 family is believed to bind exclusively to α1,4-linked glucan structures in starch. (cazypedia.org)
  • VC 2006 Wiley-Liss, Inc. a glycolipid antigen with globo-series carbohydrate core structures (Kannagi et al. (lu.se)
  • In molecular biology, a carbohydrate-binding module (CBM) is a protein domain found in carbohydrate-active enzymes (for example glycoside hydrolases). (wikipedia.org)
  • More recently, anaerobic fungi, typified by Piromyces equi, have been suggested to also synthesise a cellulosome complex, although the dockerin sequences of the bacterial and fungal enzymes are completely different. (wikipedia.org)
  • For example, the fungal enzymes contain one, two or three copies of the dockerin sequence in tandem within the catalytic polypeptide. (wikipedia.org)
  • For structural analysis of asparagine-linked carbohydrates, sugars are released from the protein backbone by enzymes such as PNGase F ( NEB #P0704 ). (neb.com)
  • A sequence alignment of 15 representative [[GH13]] enzymes possessing a CBM58 reveals that the location of this domain is invariant as an extension of the B domain (Figure 2). (cazypedia.org)
  • We have identified a novel class of peptides bound to MHC class II: mannose 6- phosphate (Man-6-P) containing glycopeptides from lysosomal enzymes. (ox.ac.uk)
  • Carbohydrate-binding module family 1 (CBM1) consists of 36 amino acids. (wikipedia.org)
  • Carbohydrate-binding module family 2 (CBM2) contains two conserved cysteines - one at each extremity of the domain - which have been shown to be involved in a disulfide bond. (wikipedia.org)
  • Carbohydrate-binding module family 3 (CBM3) is involved in cellulose binding and is found associated with a wide range of bacterial glycosyl hydrolases. (wikipedia.org)
  • Carbohydrate-binding module family 4 (CBM4) includes the two cellulose-binding domains, CBD(N1) and CBD(N2), arranged in tandem at the N terminus of the 1,4-beta-glucanase, CenC, from Cellulomonas fimi. (wikipedia.org)
  • Carbohydrate-binding module family 5 (CBM5) binds chitin. (wikipedia.org)
  • Carbohydrate-binding module family 6 (CBM6) is unusual in that it contains two substrate-binding sites, cleft A and cleft B. Cellvibrio mixtus endoglucanase 5A contains two CBM6 domains, the CBM6 domain at the C-terminus displays distinct ligand binding specificities in each of the substrate-binding clefts. (wikipedia.org)
  • Both cleft A and cleft B can bind cello-oligosaccharides, laminarin preferentially binds in cleft A, xylooligosaccharides only bind in cleft A and beta1,4,-beta1,3-mixed linked glucans only bind in cleft B. Carbohydrate-binding module family 9 (CBM9) binds to crystalline cellulose. (wikipedia.org)
  • Carbohydrate-binding module family 11 (CBM11) is found in a number of bacterial cellulases. (wikipedia.org)
  • Griffithsin's antiviral activity stems from its ability to bind terminal mannoses present in high-mannose oligosaccharides and crosslink these glycans on the surface of the viral envelope glycoproteins. (mdpi.com)
  • Glycobiology is the study of the structure, function and biology of carbohydrates, also called glycans, which are widely distributed in nature. (neb.com)
  • Gary F. Clark, PhD, is a researcher whose primary area of interest is glycobiology - the study of the structure, function and biology of carbohydrate sequences (glycans). (missouri.edu)
  • Genomic sequencing of A. fulgidus suggests that three [Ni-Fe]-CODHs are present, a "bacterial" monomeric CooS and two archaeal CdhAB-type CODHs [ 9 - 11 ]. (hindawi.com)
  • In addition, LSECtin has the ability to bind to surface glycoproteins of enveloped viruses (3, 4). (rndsystems.com)
  • Properdin "nicked" in TSR5 is unable to bind C3b but retains its overall structure and its ability to bind sulfatide. (embl.de)
  • CBMs were previously known as cellulose-binding domains. (wikipedia.org)
  • There are also four conserved tryptophans, two of which are involved in cellulose binding. (wikipedia.org)
  • These homologous CBMs are distinct in their selectivity for binding amorphous and not crystalline cellulose. (wikipedia.org)
  • Multidimensional heteronuclear nuclear magnetic resonance (NMR) spectroscopy was used to determine the tertiary structure of the 152 amino acid N-terminal cellulose-binding domain from C. fimi 1,4-beta-glucanase CenC (CBDN1). (wikipedia.org)
  • Biopolymers include the polysaccharides such as cellulose, starch, the carbohydrate polymers produced by bacteria and fungi and animal protein-based biopolymers such as wool, silk, gelatin and collagen: biopolymers, especially the carbohydrate origin, have been found very promising industrial application in … This group consists of naturally occurring polymers and chemical modifications of these polymers. (cichlidresearch.com)
  • Dystroglycan is a laminin binding component of the dystrophin-glycoprotein complex which provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. (thermofisher.com)
  • In the MGCD-265 (Glesatinib) primary S sequence, the NTD RBDs are distant from the fusion-inducing peptides. (globaltechbiz.com)
  • This study defines a novel approach for isolating rare glycan-modified peptides from MHC class II and demonstrates that very large secondary modifications are tolerated in peptides bound to MHC class II. (ox.ac.uk)
  • In anaerobic bacteria that degrade plant cell walls, exemplified by Clostridium thermocellum, the dockerin domains of the catalytic polypeptides can bind equally well to any cohesin from the same organism. (wikipedia.org)
  • Aptamers are short nucleic acid sequences capable of specific, high-affinity molecular binding. (genomicglossaries.com)
  • A synthetic, specially- designed oligonucleotide with the ability to recognize and bind a protein ligand molecule or molecules with high affinity and specificity. (genomicglossaries.com)
  • It's going to bind to that target with the same binding affinity to within a narrow range, possibly an even narrower range than for one of the batches of the innovator. (medscape.com)
  • The anaerobic bacterial dockerins are homologous to EF hands (calcium-binding motifs) and require calcium for activity whereas the fungal dockerin does not require calcium. (wikipedia.org)
  • The CBM58, active site and surface starch binding site are labeled. (cazypedia.org)
  • Site directed mutagenesis of histidine 93, aspartic acid 180, glutamic acid 205, histidine 290 and aspartic acid291 at the active site and tryptophan 279 at the raw starch binding site in barley α-amylase. (scialert.net)
  • Putative alpha-1,2-mannosidase [Carbohydrate transport and metabolism]. (unl.edu)
  • Finally, the interaction between cohesin and dockerin appears to be species specific in bacteria, there is almost no species specificity of binding within fungal species and no identified sites that distinguish different species. (wikipedia.org)
  • The resulting sequences were submitted to functional annotation against non-redundant NCBI database and Database of Anuran Defense Peptide. (biomedcentral.com)
  • Finally, although multiple amino acid substitutions of T4 abolished glycosylation at N2 but were not toxic, similar substitutions of T17 were not tolerated, suggesting that the carbohydrate moiety at N15 is critical for cell viability. (jneurosci.org)
  • Insulin present in the test sample is bound with monoclonal antibody immobilized on a magnetic solid phase and enzyme-labeled monoclonal antibody in the AIA-PACK. (cdc.gov)
  • The amount of enzyme-labeled monoclonal antibody that binds to the beads is directly proportional to the IRI concentration in the test sample. (cdc.gov)
  • A mannose 6-phosphate-containing N-linked glycopeptide derived from lysosomal acid lipase is bound to MHC class II in B lymphoblastoid cell lines. (ox.ac.uk)
  • The results obtained here indicate that the binding of IgG in NHS to the LOS and LPS samples is probably due to their carbohydrate moieties. (go.jp)
  • Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. (joplink.net)
  • The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. (thermofisher.com)
  • The identification and characterization of geometrical and physical-chemical properties in protein vacant spaces aggregates important information for steering rational drug design and functional characterization of binding and catalytic sites. (cnpem.br)
  • The majority of these domains have carbohydrate-binding activity. (wikipedia.org)
  • but in anaerobic fungi they are protein binding domains, referred to as dockerin domains. (wikipedia.org)
  • Many of the protein-protein interactions (PPI) or intra protein interactions are mediated by interactions between domains and specific patterns of sequence. (cnpem.br)
  • Moreover, the drug-DNA complexes were analyzed to investigate specific drug-DNA interactions, as well as the role of the solvent water molecules in the drug-DNA binding. (ub.edu)
  • To annotate the family with a motif model, the seed sequence was first filtered using a 0.9 fraction identity cut-off. (rfam.org)
  • Most members of this family appear to have signal sequences. (unl.edu)
  • He is focused on defining the role of carbohydrate recognition in mediating human and mammalian sperm-egg binding. (missouri.edu)
  • After activation C3b can bind covalently, via its reactive thiolester, to cell surface carbohydrates or immune aggregates. (lu.se)
  • Isolation and sequence analysis of α-amylase cDNA clone. (scialert.net)
  • By complementation and invasion assays, we demonstrate that TgMIC1 is one important player in Sia-dependent invasion and that another novel Sia-binding lectin, designated TgMIC13, is also involved. (unl.pt)
  • CBMs of microbial glycoside hydrolases play a central role in the recycling of photosynthetically fixed carbon through their binding to specific plant structural polysaccharides. (wikipedia.org)
  • Carbohydrate microarray analyses, corroborated by structural considerations, show that TgMIC13, TgMIC1, and its homologue Neospora caninum MIC1 (NcMIC1) share a preference for α2-3- over α2-6-linked sialyl-N-acetyllactosamine sequences. (unl.pt)
  • High sequence variation causes BabA functional diversity, but the underlying structural-molecular determinants are unknown. (diamond.ac.uk)
  • Here, we report a series of structural biology experiments and molecular dynamics simulations that we utilized to evaluate specific binding modes of this novel PBP inhibitor class. (bvsalud.org)
  • Human insulin is a polypeptide hormone originating in the ßeta-cells of the pancreas and serving as a principal regulator for the storage and production of carbohydrates. (cdc.gov)
  • Det innebär att en eller flera fettsyror binds till proteinets N-terminal vilket senare utgör en signal till transportsystemet (LolACDE) att proteinet ska transporteras över periplasman. (umu.se)
  • The crystal structure of SusG featuring CBM58 revealed that this domain binds to maltoheptaose as well as acarbose, a pseudotetrasaccharide amylase inhibitor. (cazypedia.org)
  • Thus the entire SusG sequence can be described from N- to C-terminus (residue numbers): A domain (43-152) - B domain (153-215) - CBM58 (215-335) - B domain (336-363) - A domain (364-607) - C domain (608-692). (cazypedia.org)
  • Additional reported applications (for the relevant formats) include: Western blotting 2,3,9 and in vitro blocking of lymphocytes binding to high endothelial venules (HEV) 2 . (biolegend.com)
  • Carbohydrate binding sites in a pancreatic α-amylase-substrate complex derived from x-ray structure analysis at 2.1Ao resolution. (scialert.net)
  • Fqo can be hypothesized to be operative during sulfate reduction with CO. The Fqo complex receives electrons from the reduced coenzyme F 420 (F 420 H 2 ), generated from the oxidative acetyl-CoA pathway, and transfers electrons to the membrane-bound respiratory chain by the reduction of menaquinone [ 16 - 18 ]. (hindawi.com)
  • Properdin lacking TSR4 is unable to stabilize the C3b-Bb complex but is able to bind C3b and sulfatide, and shows the presence of monomers and dimers in an electron microscope. (embl.de)
  • Properdin without TSR3 is able to stabilize the C3b-Bb complex, to bind C3b and sulfatide, and forms dimers, trimers, and tetramers. (embl.de)
  • The N-linked carbohydrate of properdin is not required for oligomerization or stabilization of the C3b-Bb complex. (embl.de)
  • The results implicate TSR5 in both C3b and sulfatide binding, and suggest that TSR4 may also be involved in stabilization of the C3b-Bb complex. (embl.de)
  • It also binds sulfated glycoconjugates, such as sulfatide, in vitro. (embl.de)
  • In the context of influenza HA cleavage, HAT has a broader cleavage capacity than TMPRSS2, proteolyzing HA both in virus-producing cells and in progeny viruses bound to target cell receptors [96]. (globaltechbiz.com)
  • Polymers, including biopolymers, are made of repetitive units called monomers.Biopolymers inherently have a well defined structure: The exact chemical composition and the sequence in which these units are arranged is called the primary structure. (cichlidresearch.com)