Figure 1: Green versus red fluorescence cytograms and corresponding alpha t frequency histograms of Acridine Orange (AO) stained bull semen samples prepared and measured by the Sperm Chromatin Structure Assay (SCSA). (purdue.edu)
The fluorescence intensity of DAPI molecules bound to double-stranded DNA is increased by about 20 times, and it is commonly observed with fluorescence microscopy, and the amount of DNA can be determined based on the intensity of fluorescence. (medchemexpress.com)
DFHBI-1T binds to RNA aptamers (Spinach, Spinach2, iSpinach, and Broccoli) and causes specific fluorescence and lower background fluorescence. (medchemexpress.com)
IF workers must detect fluorescence from both RNA and DNA-bound Acridine Orange a longpass set such as 49012 is recommnded. (cinemasleep.com)
4. Yagci A, Murk W, Stronk J, Huszar G. Spermatozoa bound to solid state hyaluronic acid show chromatin structure with high DNA chain integrity: an acridine orange fluorescence study. (dergipark.org.tr)
There are a number of DNA-intercalating fluorescent compounds such as acridine orange, quinacrine mustard, acriflavin, mithramycin, auramine O, olivomycin A, and ethidium bromide, which bind very specifically to the nucleus. (taylorfrancis.com)
Autophagy was detected using acridine orange staining and real-time PCR for Lc3 and Beclin1 genes expression. (ijpsonline.com)
When acridine orange (AO) stained sperm are exposed to 488 nm laser light, AO intercalated into ds DNA fluoresces green and AO bound to ss DNA fluoresces red. (purdue.edu)
Acridine Orange hydrochloride is a cell-penetrable nucleic acid-selective fluorescent dye . (medchemexpress.com)
We prepared medullary membrane vesicles highly enriched for the H + -ATPase to examine possible functional interactions of G proteins with the H + -ATPase by the acridine orange method. (wustl.edu)
1972)Biopolyrners 11,645-6521 used electro-optical measurements to show that methylene blue (MB),like acridine orange, in its DNA complex is oriented more or less perpendicular to the helix axis as expected if intercalated. (ac.ir)
The dihedral angle of the MB pairs, corresponding to a left-handed helix, is opposite to that with acridine orange and proflavine on DNA, indicating that the latter ligands bind to DNA in a different way. (ac.ir)
Compounds5
Site-moiety mapping of compounds from the diversity set revealed three binding anchors encompassing both hydrogen bonding and van der Waals interaction. (peerj.com)
Molecular docking revealed that compounds 13 , 5 and 28 exhibited the lowest binding energies of −12.2, −12.0 and −12.0 kcal/mol, respectively, against human AChE, which is modulated by hydrogen bonding, π - π stacking and hydrophobic interaction inside the binding pocket. (peerj.com)
Previous studies by our laboratory have identified a series of 9-amino acridine compounds that block the catalytic cycle of topoisomerase II resulting in apoptosis and cell death in a variety of cancer cell lines. (umn.edu)
Pharmacokinetic data collected at time intervals following a 60 mg/kg oral dose of acridine 1 and 2 showed both compounds penetrate the blood-brain barrier yielding peak concentrations of 0.25 μM and 0.6 μM, respectively. (umn.edu)
Previous efforts have led to compounds that disable pumps by inhibiting one of the drug binding sites in the inner membrane component of the pump, but such compounds have proven toxic and overly specific. (ku.edu)
Fluorescent3
Mithramycin is inert towards RNA and protein but fluoresces yellow when bound to double stranded DNA in the presence of Mg. The specific binding and fluorescent properties of mithramycin make it possible to stain fungal nuclei in a rapid one step procedure. (taylorfrancis.com)
Phenanthridine is a nitrogen heterocyclic compound that is the basis of DNA-binding fluorescent dyes through intercalation. (chemchart.com)
Proteins5
DNA-binding proteins (DNA-BPs) and RNA-binding proteins (RNA-BPs) have critical roles in living cells in all kingdoms of life. (nature.com)
Hudson, W.H. & Ortlund, E.A. The structure, function and evolution of proteins that bind DNA and RNA. (nature.com)
In particular, the cytotoxic activity, conformational distortions, recognition by DNA-binding proteins, and repair mechanisms are dependent on the arene. (warwick.ac.uk)
Guanosine 5′-triphosphate (GTP)-binding proteins (G proteins) are expressed in a heterogeneous manner in the mammalian kidney. (wustl.edu)
In contrast, cells with dead or damaged membranes allow the dye to penetrate, binding to intracellular proteins and staining the cells. (logosbio.com)
Stain1
It is a non-specific stain which preferentially binds to chitin and cellulose in cell walls and as such is also used to visualize plant cells. (cinemasleep.com)
Derivatives2
DNA has a strong affinity for many heterocyclic aromatic dyes, such as acridine and its derivatives. (warwick.ac.uk)
2. Mrinal Kanti Si, Sumit Kumar Pramanik, Bishwajit Ganguly: ''Tuning the ring strain effect in acridine derivatives on binding affinity with G-quadruplex-DNA: A computational and experimental study. (owsd.net)
Affinity2
The MIP/BPNS-AuNPs show a high binding affinity for PEF, even in the presence of structural analogs, and maintain stable voltammetric signals for at least 35 d. (bvsalud.org)
Lerman in 1961 first proposed intercalation as the source of this affinity, and this mode of DNA binding has since attracted considerable research scrutiny. (warwick.ac.uk)
Vitro1
In vitro, it binds to some environmental mutagens such as the polycyclic aromatic hydrocarbons benzo[a]pyrene and dibenzo[a,i]pyrene. (wikipedia.org)
Intercalation1
Metal complexes with σ-bonded aromatic side arms can act as dual-function complexes: they bind to DNA both by metal coordination and through intercalation of the attached aromatic ligand. (warwick.ac.uk)
Sperm2
The present study was conducted to assess the sperm morphology and DNA integrity of frozen thawed buffalo semen treated with heparin binding protein (HBP). (ijcmas.com)
Fertility testing and ICSI sperm selection by hyaluronic acid binding: clinical and genetic aspects. (dergipark.org.tr)
Double stranded1
It binds to the AT base pair of the double-stranded DNA minor groove, and one DAPI molecule can occupy three base pair positions. (medchemexpress.com)
Protein1
Motion, G.B., Howden, A.J.M., Huitema, E. & Jones, S. DNA-binding protein prediction using plant specific support vector machines: validation and application of a new genome annotation tool. (nature.com)
Efflux3
Antibiotic resistance is correlated with overexpression of the acridine efflux pump. (ku.edu)
Specifically, by targeting oligomerization we can stop all efflux through the pump, not just the antibiotics that interact with one of the multiple acridine pump binding sites. (ku.edu)
Our central hypothesis is that we can disrupt assembly of the outer membrane β-barrel component of efflux pumps, by binding their interface strands with β-hairpin peptides or β-hairpin mimetics similar to the interfacial strands' native binding partners. (ku.edu)
Synthesis1
Design, synthesis, and DNA binding properties of bifunctional intercalators. (chemchart.com)
Inhibitor1
Moreover, targeting oligomerization instead of targeting one of the two binding sites broadens the applicability of the inhibitor. (ku.edu)
Buffer2
Cells had been resuspended in Annexin V binding buffer (BD Pharmingen NORTH PARK CA USA) to a focus of 1×106 cells/ml. (biongenex.com)
PI was after that spiked into 400 μl Annexin V binding buffer and added instantly towards the cell suspension system and subsequently examined utilizing a Catechin FACSCaliber? (biongenex.com)
Interactions2
These aromatic side arms introduce new modes of DNA binding, involving mutual interactions of functional groups held in close proximity. (warwick.ac.uk)
Arene−nucleobase stacking interactions can have a significant influence on both the kinetics and thermodynamics of DNA binding. (warwick.ac.uk)
Sites1
BPNS-AuNPs significantly enhance the black phosphorus (BP) stability and electrochemical activity and offer a larger surface area to accommodate more imprinted sites for selective PEF binding. (bvsalud.org)
Interaction2
0.05),a weak monomeric CD with the same shape as the absorption curve is observed, while at higher binding ratios, a strong exciton CD dominates due to interaction between pairs of MB ligands. (ac.ir)
The exciton effect at high binding ratios, observed both in CD and in LD, can be interpreted in terms of an interaction between an intercalated and a nonintercalated MB. (ac.ir)
Cells1
Annexin V binding assay MCF-7 cells were treated with TAM and NDAM alone or in mixture for 72 h. (biongenex.com)
High1
High-precision flow linear dichroism (LD) here confirms that MB is coplanar with the DNA bases at low dyeDNA binding ratios and low ionic strengths. (ac.ir)
Regions2
Importantly, we identify putative ESRRa binding elements in upstream regions of sox9 to which ESRRa can directly bind, indicating that Esrra may directly regulate sox9 expression. (nature.com)
7-Aminoactinomycin D selectively binds to GC regions of the DNA. (medchemexpress.com)
Cell1
In addition, cell apoptosis was measured by double staining, DNA fragmentation and annexin V-fluorescein isothiocyanate binding assays. (ijpsonline.com)
Model1
We present a recurrent neural network for feature binding and sensory segmentation: the competitive -layer model (CLM). (lookformedical.com)