• histone
  • In a previous study, we provided the first in vivo evidence that a specific fragment of ER-α promoter is bound by pRb2/p130-E2F4/5-histone deacetylase 1 (HDAC1)-SUV39H1-p300 and pRb2/p130-E2F4/5-HDAC1-SUV39H1-DNA methyltransferase 1 (DNMT1) complexes in ER-α-positive and ER-α-negative breast cancer cells, respectively ( 14 ). (aacrjournals.org)
  • signaling pathway
  • Smads were first discovered in Drosophila, in which they are known as mothers against dpp (Mad), through a genetic screen for dominant enhancers of decapentaplegic (dpp), the Drosophila version of TGF-B. Studies found that Mad null mutants showed similar phenotypes to dpp mutants, suggesting that Mad played an important role in some aspect of the dpp signaling pathway. (wikipedia.org)
  • p130
  • Here, we showed that the presence of a specific pRb2/p130 multimolecular complex on the ER-α promoter strongly correlates with the methylation status of this gene. (aacrjournals.org)
  • viral
  • There have, however, been many reports of difficulties in establishing functioning stable cell lines due to the cytotoxic effects of expressing high levels of the tetracycline transactivator, tTA, from a strong viral promoter. (biomedcentral.com)
  • progression
  • Our previous work (5) and other studies (4) suggest that RASSF1A may indeed have multiple functions, which affect tumorigenesis ranging from inhibiting cell cycle progression to influencing other important variables of tumorigenesis, including cell adhesion, cell migration, angiogenesis, transcription, and apoptosis. (aacrjournals.org)
  • whereas
  • However, electrophoretic mobility shift assays indicate that knockdown of endogenous RASSF1A in HB2 and HeLa cells leads to a reduction in the binding capacity of p120 E4F to the cyclin A2 promoter, whereas in the A549 clone stably expressing RASSF1A the binding capacity is increased. (aacrjournals.org)
  • For example, factors of the Ikaros family specifically favor differentiation down the lymphoid pathway ( 3 ), whereas other factors, such as GATA-1 and C/EBPα, favor differentiation down the myeloid pathway ( 4 , 5 ). (jimmunol.org)
  • Overexpression of E2F4 promotes terminal differentiation of cells in vitro ( 16 ), whereas E2F4-deficient animals show reduced neonatal viability, with very few animals surviving to adulthood ( 15 , 17 ). (jimmunol.org)
  • contrast
  • In contrast to cells produced using the CMV and PGK promoters, those produced using the EF1α and TRE promoters expressed high levels of β-galactosidase in a tetracycline-dependent manner. (biomedcentral.com)
  • modulate
  • FLP recognizes a distinct cis -regulatory element that overlaps with that of the cell cycle activator E2F-DP in the CDKB1;1 promoter, suggesting that these MYBs may also modulate E2F-DP pathways. (plantcell.org)
  • nucleus
  • R/Co-Smads are primarily located in the cytoplasm, but accumulate in the nucleus following TGF-B signaling, where they can bind to DNA and regulate transcription. (wikipedia.org)
  • found
  • We analyzed the impact of E2F4 deficiency on early steps in mouse hematopoietic development, and found defects in early hematopoietic progenitor cells that were propagated through common lymphoid precursors to the B and T lineages. (jimmunol.org)
  • Among these, E2F4 is found at high concentrations in hematopoietic cells ( 15 ). (jimmunol.org)
  • genetic
  • The pathogenesis of breast cancer is poorly understood, but epidemiologic, molecular, and clinical genetic studies have implicated factors and defined molecular markers that are associated with an increased risk of developing breast cancer ( 15 , 16 ). (aacrjournals.org)
  • The MS etiology is still complex and mostly unclear, with genetic and environmental factors possibly influencing the susceptibility and/or the outcomes of the disease. (pubmedcentralcanada.ca)
  • unclear
  • It is still unclear how environmental signals ( 1 ) and lineage-specific transcription factors work together to control the frequency with which dividing HSC either undergo self-renewal or commit to one or the other lineage. (jimmunol.org)
  • complex
  • This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. (cancerindex.org)
  • growth
  • Consistently, at the molecular level, activation of growth factor signaling pathways by EGFR / ERBB / AKT and a switch from phospho-Ser118 (pS118)- to pS167-ER α were observed during MCF7-LTED adaptation. (nature.com)
  • shown
  • Recently, E2F4 has been shown to play a role during fetal erythropoiesis, explaining the anemia that is seen in the fetuses and neonates ( 18 ). (jimmunol.org)