• assays
  • However, electrophoretic mobility shift assays indicate that knockdown of endogenous RASSF1A in HB2 and HeLa cells leads to a reduction in the binding capacity of p120 E4F to the cyclin A2 promoter, whereas in the A549 clone stably expressing RASSF1A the binding capacity is increased. (aacrjournals.org)
  • p130
  • Here, we showed that the presence of a specific pRb2/p130 multimolecular complex on the ER-α promoter strongly correlates with the methylation status of this gene. (aacrjournals.org)
  • structurally similar
  • Smads (or SMADs) comprise a family of structurally similar proteins that are the main signal transducers for receptors of the transforming growth factor beta (TGF-B) superfamily, which are critically important for regulating cell development and growth. (wikipedia.org)
  • progression
  • Our previous work (5) and other studies (4) suggest that RASSF1A may indeed have multiple functions, which affect tumorigenesis ranging from inhibiting cell cycle progression to influencing other important variables of tumorigenesis, including cell adhesion, cell migration, angiogenesis, transcription, and apoptosis. (aacrjournals.org)
  • sequence
  • Another myogenic factor that is highly related to MyoD in both amino acid sequence and affinity to E-box sites is Myf5 ( Tapscott, 2005 ). (rupress.org)
  • signals
  • It is still unclear how environmental signals ( 1 ) and lineage-specific transcription factors work together to control the frequency with which dividing HSC either undergo self-renewal or commit to one or the other lineage. (jimmunol.org)
  • pathway
  • Smads were first discovered in Drosophila, in which they are known as mothers against dpp (Mad), through a genetic screen for dominant enhancers of decapentaplegic (dpp), the Drosophila version of TGF-B. Studies found that Mad null mutants showed similar phenotypes to dpp mutants, suggesting that Mad played an important role in some aspect of the dpp signaling pathway. (wikipedia.org)
  • cdc6
  • In C2C12 and primary mouse myoblasts, we show that MyoD can occupy an E-box within the promoter of Cdc6 and that this association, along with E2F3a, is required for its activity. (rupress.org)
  • Although this scenario has yet to be demonstrated in satellite cells, it is most likely that analogous mechanisms would occur, and if so, it is possible that MyoD could be involved in inducing transcription from either Cdc6 or MCM2 in these cells after leaving quiescence. (rupress.org)
  • metabolic
  • These instant metabolic changes bypass the signaling pathways which are required for activating the TFs, but instead, they just alter the catalytic activities of the JMJDs, which are already assigned to specific histones through their interactions with the DNA-binding TFs. (bmbreports.org)
  • pathways
  • Consistently, at the molecular level, activation of growth factor signaling pathways by EGFR / ERBB / AKT and a switch from phospho-Ser118 (pS118)- to pS167-ER α were observed during MCF7-LTED adaptation. (nature.com)
  • nucleus
  • R/Co-Smads are primarily located in the cytoplasm, but accumulate in the nucleus following TGF-B signaling, where they can bind to DNA and regulate transcription. (wikipedia.org)
  • found
  • We analyzed the impact of E2F4 deficiency on early steps in mouse hematopoietic development, and found defects in early hematopoietic progenitor cells that were propagated through common lymphoid precursors to the B and T lineages. (jimmunol.org)
  • Among these, E2F4 is found at high concentrations in hematopoietic cells ( 15 ). (jimmunol.org)
  • molecular
  • The pathogenesis of breast cancer is poorly understood, but epidemiologic, molecular, and clinical genetic studies have implicated factors and defined molecular markers that are associated with an increased risk of developing breast cancer ( 15 , 16 ). (aacrjournals.org)
  • specific
  • This approach requires the use of specific gene promoters, which in some circumstances may represent a problematic issue due to ectopic expression or poorly characterized control elements. (frontiersin.org)
  • genomic
  • Encyclopedia of DNA Elements (ENCODE) is another large-scale genomic project that aims to map the genome-wide binding sites of TFs using ChIP-seq [ 3 ]. (hindawi.com)