• Lysis buffer is prepared by adding 10 μl beta-mercaptoethanol (ß-ME) per ml of RLT buffer. (nih.gov)
  • Supplied in SDS sample buffer containing 5% beta-mercaptoethanol. (biocheminfo.org)
  • Bilateral tissue of the same brain region from each rat was immediately transferred into 350 ul Buffer RLT (containing beta-mercaptoethanol) and placed on dry ice. (nih.gov)
  • The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). (creativebiomart.net)
  • Q6: What is the expiration of the Viral RNA Buffer when beta-mercaptoethanol has been added? (zymoresearch.de)
  • The Viral RNA Buffer is guaranteed for one year from the date of purchase even with the addition of beta-mercaptoethanol. (zymoresearch.de)
  • To help prevent this in future preps, it's best to add beta-mercaptoethanol to the Viral RNA buffer, and/or implement a Proteinase K digestion step. (zymoresearch.de)
  • For expansion, Pro-B cells were cultured on OP9 stromal cells supplemented with 10ng/mL KIT ligand, 10ng/mL Fms-like tyrosine kinase 3 ligand (FLT3L), and 10ng/mL Interleukin-7 in Optimem supplemented with 10% FCS, Beta-mercaptoethanol, HEPES and Penstrep. (nih.gov)
  • Addition of beta-mercaptoethanol is recommended to enhance sample lysis, but can be substituted with dithiothreitol (DTT, final concentration of 10 mM). (mbpinc.net)
  • The only element that the side chains of these residues have in common with the WT arginine is the set of three carbon atoms at the C(alpha), C(beta), and C(gamma) positions. (rcsb.org)
  • The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). (creativebiomart.net)
  • The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% Beta-mercaptoethanol. (abcam.cn)
  • O Low concentration of beta mercaptoethanol Oxygen OHigh Concentration of beta-mercaptoethanol. (tutorbin.com)
  • Mouse Age 7 Days Lung Tissue Lysate from Innovative Research is provided as a Liquid buffered in SDS sample buffer containing 5% beta-mercaptoethanol with a concentration of 2mg/ml. (innov-research.com)
  • This protein, which originates in the maternal endometrium, was measured in allantoic fluid by a specific double-antibody radioimmunoassay and by its beta-mercaptoethanol-activated acid phosphatase activity. (tamu.edu)
  • The most common types in China are alpha and beta thalassemia. (hindawi.com)