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MutagenesisMutagenesis, Site-DirectedMutagenesis, InsertionalMolecular Sequence DataAmino Acid SequenceMutationBase SequenceBinding SitesModels, MolecularEscherichia coliAmino Acid SubstitutionStructure-Activity RelationshipSequence Homology, Amino AcidDNA Transposable ElementsRecombinant ProteinsPlasmidsProtein Structure, TertiaryCloning, MolecularBacterial ProteinsKineticsProtein ConformationProtein BindingCysteineProtein Structure, SecondarySequence AlignmentGenes, BacterialPoint MutationMutagensEthylnitrosoureaSubstrate SpecificityCatalysisAlanineDNA PrimersCell LineSOS Response (Genetics)HistidineConserved SequenceDNA Mutational AnalysisCatalytic DomainEscherichia coli ProteinsCrystallography, X-RayGenetic Complementation TestTransfectionRestriction MappingEthyl MethanesulfonateDNA, BacterialSequence DeletionRecombinant Fusion ProteinsProtein EngineeringOligodeoxyribonucleotidesLysinePhenotypeDirected Molecular EvolutionAspartic AcidDNAAmino Acid MotifsEnzyme StabilityMutant ProteinsPromoter Regions, GeneticUltraviolet RaysSaccharomyces cerevisiaeTranscription, GeneticAsparagineCricetinaeGene Expression Regulation, BacterialGenetic VectorsDNA-Binding ProteinsSerineCOS CellsPolymerase Chain ReactionDNA RepairDNA-Directed DNA PolymeraseArginineCircular DichroismTranscription FactorsSequence Analysis, DNADimerizationLigandsTryptophanGene DeletionCHO CellsCercopithecus aethiopsCodonElectrophoresis, Polyacrylamide GelMutagenicity TestsAmino AcidsOperonHydrogen-Ion ConcentrationGlycosylationGene LibraryMembrane ProteinsRecombination, GeneticCarrier ProteinsDNA DamageTransformation, BacterialHydrolysisTransformation, GeneticMethylnitronitrosoguanidineGenetic EngineeringHydrogen Bonding
GenesTransposon-based insertional mutagenesisSite-directed muMutationsAgrobacteriumGeneRetrovirusesVirusesResistanceHumanResult
Genes3
  • An alternative strategy for insertional mutagenesis has been used in vertebrate animals to find genes that cause cancer. (wikipedia.org)
  • While the selective inactivation of Ophiostoma genes by insertional mutagenesis remains a challenge, an alternative approach based on RNA interference is now available for down-regulating the expression of targeted genes. (sisef.it)
  • The researchers used a molecular biology technique called gene-trap insertional mutagenesis to identify hundreds of human genes that enable viruses to hijack a human cell, but are not necessary for the cell itself to survive. (infectioncontroltoday.com)
Transposon-based insertional mutagenesis1
  • The contributors describe strategies for producing genetic models, including transgenic germline models, gene knockouts and knockins, and conditional and inducible systems, as well as models derived using transposon-based insertional mutagenesis, RNA interference, viral-mediated gene delivery, and chemical carcinogens. (cshlpress.com)
Site-directed mu1
  • Eucaryotic elongation factor 2 (EF-2) contains a post-translationally modified histidine residue termed diphthamide that is specifically ADP-ribosylated by diphtheria toxin (DT) or Pseudomonas exotoxin A. To analyze the potential physiological role of ADP-ribosylation of EF-2 by cellular ADP-ribosyl transferase, we constructed DT-resistant, non-ADP-ribosylatable Saccharomyces cerevisiae EF-2 by site-directed mutagenesis and expressed the mutant EF-2 in yeast. (nih.gov)
Mutations5
  • In molecular biology, insertional mutagenesis is the creation of mutations in DNA by the addition of one or more base pairs. (wikipedia.org)
  • Such insertional mutations can occur naturally, mediated by viruses or transposons, or can be artificially created for research purposes in the lab. (wikipedia.org)
  • Historically, lentiviral vectors included strong viral promoters which had a side effect of insertional mutagenesis, nuclear DNA mutations that effect the function of a gene. (wikipedia.org)
  • The CDC defines DNA vaccines as "purified plasmid preparations containing one or more DNA sequences capable of inducing and/or promoting an immune response against a pathogen," yet there is no proof that this really works as stated, and research shows that when these sequences are injected they can cause "insertional mutagenesis," which means gene and cell mutations can result - and that's also the definition of carcinogenesis, or "cancer. (ipetitions.com)
  • Comprehensive Functional Analysis of the Enterococcus faecalis Core Genome Using an Ordered, Sequence-Defined Collection of Insertional Mutations in Strain OG1RF. (cdc.gov)
Agrobacterium2
  • An insertional mutagenesis protocol using Agrobacterium tumefacience-mediated transformation is developed to transform S. sclerotiorum. (usda.gov)
  • Advancing Functional Genetics Through Agrobacterium -Mediated Insertional Mutagenesis and CRISPR/Cas9 in the Commensal and Pathogenic Yeast Malassezia . (bvsalud.org)
Gene5
  • Insertional mutagenesis is possible whether the virus is of the self-inactivating types commonly used in gene therapy or competent to replicate. (wikipedia.org)
  • Insertional inactivation is a technique used in recombinant DNA engineering where a plasmid (such as pBR322) is used to disable the expression of a gene. (wikipedia.org)
  • A mutant library was created with insertional mutagenesis via electroporation in order to transform a BASTA resistance gene into conidial nuclei. (uni-muenchen.de)
  • Mouse insertional mutagenesis experiments support the designation of JARID2 as a cancer causing gene. (sanger.ac.uk)
  • Reference: Cheng F, Murray JL, Zhao J, Sheng J, Zhao Z, Rubin DH (2016) Systems Biology-Based Investigation of Cellular Antiviral Drug Targets Identified by Gene-Trap Insertional Mutagenesis. (infectioncontroltoday.com)
Retroviruses1
  • tax causes malignant transformation not through integration and insertional mutagenesis, as many retroviruses do, but by inhibition of DNA repair (base excision pathway) and trans-activating disruption of cellular growth control mechanisms ( 2 ). (cdc.gov)
Viruses2
  • Because many viruses integrate their own genomes into the genomes of their host cells in order to replicate, mutagenesis caused by viral infections is a fairly common occurrence. (wikipedia.org)
  • Not all integrating viruses cause insertional mutagenesis, however. (wikipedia.org)
Resistance1
Human1
  • There is an example of an insertional mutagenesis event caused by a retrotransposon in the human genome where it causes Fukuyama-type muscular dystrophy. (wikipedia.org)
Result1
  • What you might get is what's known as "insertional mutagenesis" and it can result in all sorts of bad things happening. (civileats.com)