The enzyme chorismate lyase (EC 4.1.3.40) catalyzes the first step in ubiquinone biosynthesis, the removal of pyruvate from chorismate, to yield 4-hydroxybenzoate in Escherichia coli and other Gram-negative bacteria. (wikipedia.org)
The systematic name of this enzyme class is chorismate pyruvate-lyase (4-hydroxybenzoate-forming). (wikipedia.org)
This enzyme has Heat-like repeats that are characteristic for the E/F-type lyases, but it had not chromophore-attaching activity. (uni-muenchen.de)
The spore photoproduct lyase is a radical SAM enzyme, which repairs 5-(α-thyminyl)-5,6-dihydrothymidine. (cipsm.de)
This gene provides instructions for making an enzyme called adenylosuccinate lyase, which performs two steps in the process that produces purine nucleotides. (medlineplus.gov)
Since the transfer is intramolecular, the reaction is an elimination and, hence, the enzyme is a lyase, belonging in EC 4. (enzyme-database.org)
The optimal pH of this enzyme is 8.5 and the optimal temperature is 45¿C. It is a salt-tolerant alginate lyase with an optimal activity at 0.6 M NaCl. (up.pt)
The main breakdown products of the enzyme on alginate were disaccharides, trisaccharides, and tetrasaccharides, which demonstrated that this enzyme acted as an endo-type alginate lyase. (up.pt)
The irreversible breakdown of SIP is catalysed by sphingosine-1-phosphate lyase (SIPL), a pyridoxal 5'-phosphate (PLP) dependent enzyme that catalyses the retro-aldol-like cleavage of SIP to (2E)-hexadecenal (2E-HEX) and phosphoethanolamine (PE). (ed.ac.uk)
S1PL is a PLP Fold type I carbon-carbon bond lyase belonging to the Group II PLP-dependent decarboxylase subfamily and is the terminal enzyme in sphingolipid metabolism. (ed.ac.uk)
In enzymology, a GDP-mannose 4,6-dehydratase (EC 4.2.1.47) is an enzyme belongs to the family of lyases, specifically the hydro-lyases, which cleave carbon-oxygen bonds. (creative-biolabs.com)
Exploring molecular determinants of polysaccharide lyase family 6-1 enzyme activity. (ox.ac.uk)
ATP citrate-lyase (EC 2.3.3.8 ) [ 1 ], the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues, catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with the concomitant hydrolysis of ATP to ADP and phosphate. (expasy.org)
Citrate lyase9
Acetylation of prosthetic group (2-(5''-phosphoribosyl)-3'- dephosphocoenzyme-A) of the gamma subunit of citrate lyase. (string-db.org)
Catalyzes the formation of 2-(5''-triphosphoribosyl)-3'- dephosphocoenzyme-A, the precursor of the prosthetic group of the holo- acyl carrier protein (gamma chain) of citrate lyase, from ATP and dephospho-CoA. (string-db.org)
Transfers 2-(5''-triphosphoribosyl)-3'-dephosphocoenzyme-A on a serine residue to the apo-acyl carrier protein (gamma chain) of the citrate lyase to yield holo-acyl carrier protein. (string-db.org)
ATP-citrate lyase is a tetramer of identical subunits. (expasy.org)
We developed three signature patterns for these enzymes, the first corresponds to a glycine-rich conserved region, located in the second half of ATP citrate lyase and in the α subunits of succinyl-CoA ligases and malate-CoA ligase. (expasy.org)
The last pattern corresponds to a conserved region located in the first half of ATP citrate lyase and in the β subunits of succinyl-CoA ligases and malate-CoA ligase. (expasy.org)
Cloning and expression of a human ATP-citrate lyase cDNA. (expasy.org)
Finally, blocking lipogenesis with fatty acid synthase inhibitors (cerulenin and C75) and siRNA-mediated silencing of SREBP1 and ATP citrate lyase (ACL) significantly attenuated GSK3 inhibition-induced senescence. (koreamed.org)
SREBP1 is a master transcription factor that regulates the expression of several lipogenic enzymes, including ATP citrate lyase (ACL), acetyl-CoA carboxylase (ACC), and fatty acid synthase (FAS) [ 11 ]. (koreamed.org)
Polysaccharide lyases1
compared to other polysaccharide lyases such as pectate lyases, which tend to have rather basic pH optima. (cazypedia.org)
Alginate lyase4
Title: Purification and characterization of a novel alginate lyase from a marine streptomyces species isolated from seaweed Abstract: Alginate, a natural polysaccharide derived from brown seaweed, is finding multiple applications in biomedicine via its transformation through chemical, physical, and, increasingly, enzymatic processes. (up.pt)
In this study a novel alginate lyase, AlyDS44, was purified and characterized from a marine actinobacterium, Streptomyces luridiscabiei, which was isolated from decomposing seaweed. (up.pt)
AlyDS44 is a bifunctional alginate lyase, active on both polyguluronate and polymannuronate, though it preferentially degrades polyguluronate. (up.pt)
Polysaccharide substrate specificity analysis indicated that ALW1 could produce extracellular alginate lyase, laminarinase, fucoidanase and cellulase. (springeropen.com)
Glycoside2
He contributed isolation and enzymatic characterization of polysaccharide lyase and glycoside hydrolase belonging new families ( PL20 and GH128 ) [ 1 , 2 , 3 ]. (cazypedia.org)
They belong to the three glycoside hydrolase Families 13, 70 and 77, forming a clan GH-H in the frame of the sequence-based classification of glycosidases. (go.jp)
To decipher the mode of recognition and action of the enzymes belonging to subfamily PL6_1, we solved the crystal structures of Pedsa0632, Patl3640, Pedsa3628 and Pedsa3807, which all show different substrate specificities and mode of action (endo-/exolyase). (ox.ac.uk)
Characterization2
The comprehensive characterization of TftG helps not only for identifying effective mechanisms for chloroaromatic dechlorination but also for understanding the functions of YCII superfamily members, which we propose to be lyases. (rcsb.org)
cDNA cloning, purification, and characterization of mouse liver selenocysteine lyase. (unipr.it)
The 181-amino-acid sequence of NbaB is 35% identical to the 4-hydroxylaminobenzoate lyases (PnbBs) of various 4-nitrobenzoate-assimilating bacteria, e.g. (canada.ca)
Cpr12, encoded by a gene that was originally proposed to be outside the gene cluster and sharing similarity to proteins annotated as ADC lyase, was revealed to catalyze the elimination of pyruvate to form PABA. (uky.edu)
Superfamily3
AOS belongs to the superfamily of cytochrome P450 enzymes and is named CYP74A. (upm.es)
Protein phosphatase 2A (PP2A) belongs to the superfamily of phosphoprotein phosphatases (PPPs) and catalyzes protein dephosphorylation by hydrolyzing Ser/Thr-linked phosphate ester bonds ( Heroes,2013 ). (eu.org)
The F35H belongs to the CYP75 superfamily of P450 enzymes. (chk1inhibitor.com)
Amino3
The highly conserved regions of its amino acid sequences indicated that AlyDS44 belongs to the polysaccharide lyase family 7. (up.pt)
ALs, belonging to the enolase family, specifically use unsaturated carboxylates as substrates for the hydroamination reaction to generate unnatural amino acids and their derivatives (Turner 2011 ). (springeropen.com)
The depletion of DOM over time was strongly correlated with increases in expression of many genes associated with heterotrophy (e.g., amino acid, fatty acid, and carbohydrate metabolism) belonging to a Hydrogenophaga strain that accounted for a relatively large percentage (~8%) of the metatranscriptome. (frontiersin.org)
Pyruvate1
It is also called chorismate pyruvate lyase (4-hydroxybenzoate-forming) and it is also abbreviated several different ways: CPL, CL, and ubiC. (wikipedia.org)
Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. (string-db.org)
The polysaccharide lyase family 6 (PL6) represents one of the 41 polysaccharide lyase families classified in the CAZy database with the vast majority of its members being alginate lyases grouped into three subfamilies, PL6_1-3. (ox.ac.uk)
Recent studies showed that α-glucosidases could be divided into two groups, family I and family II, in which family I enzymes belong to the α-amylase family. (go.jp)
The mammalian enzymes included in this family belong to a lineage distinct from that of bacterial and plant enzymes. (unipr.it)
structurally, it belongs to the homodimeric NADH:flavin mononucleotide (FMN) oxidoreductase-like fold family. (canada.ca)
Protein1
Cpr37, which belongs to the adenylation domain protein in the nonribosomal peptide synthase (NRPS), subsequently activates PABA and loads it to holo-Cpr36 Two proteins Cpr34 and Cpr35 work in concert to catalyze decarboxylative condensation between a thioester linked PABA and malonyl- S -acyl carrier protein (ACP) during aromatic polyketide biosynthesis catalyzed by type II polyketide synthases. (uky.edu)
Substrate4
Obviously, this lyase has a much broader substrate specificity than the E/F-type lyases, but it has high site-specificity, attaching the chromophore exclusively to the Cys-84 (consensus sequence) binding site of the APC subunits. (uni-muenchen.de)
Another CYP450 in chloroplasts, hydroperoxide lyase (HPL, CYP74B), competes with AOS for the common substrate. (upm.es)
Despite the broad substrate spectrum of methylaspartate lyase (MAL), some bulky substrates, such as caffeic acid, cannot be effectively accepted. (springeropen.com)
From comparative studies with existing structures of PL6_1 alginate lyases, we observe that in the right-handed parallel β-helix fold shared by all these enzymes, the substrate-binding site harbors the same overall conserved structures and organization. (ox.ac.uk)
Burkholderia1
Identification and characterisation of novel sphingosine-1-phosphate lyases in Burkholderia ssp. (ed.ac.uk)
Biosynthetic1
Introduction of these transcription factors under control of the fruit specific E8 promoter increased the expression of most of the structural genes in the biosynthetic pathway in the tomato fruit, including phenylalanine ammonia lyase, chalcone isomerase and F35H. (chk1inhibitor.com)
Proteins1
Chromium - Usually found in a variety of food sources, including proteins and vegetables, this element belongs to the supplement's trace minerals group. (patosdategardens.com)
Deficiency7
Adenylosuccinate lyase deficiency is a neurological disorder that causes brain dysfunction (encephalopathy) leading to delayed development of mental and movement abilities (psychomotor delay), autistic characteristics that affect communication and social interaction, and seizures. (medlineplus.gov)
Adenylosuccinate lyase deficiency is classified into three forms based on the severity of the signs and symptoms. (medlineplus.gov)
Adenylosuccinate lyase deficiency type I (also known as the severe form) is the most common. (medlineplus.gov)
In individuals with adenylosuccinate lyase deficiency type II (also known as the moderate or mild form), development is typically normal for the first few years of life but then slows. (medlineplus.gov)
damage to brain tissue caused by one or both of these substances likely underlies the neurological problems that occur in adenylosuccinate lyase deficiency. (medlineplus.gov)
which despite belonging to [[PL1]], have diverged significantly from the pectate lyases. (cazypedia.org)
Reaction2
In addition, the correct structures of the products from all three lyase types have been verified, and evidence was obtained for the reaction mechanisms. (uni-muenchen.de)
The first coupled assay uses a human bone phosphatase, PHOSPHOl, which is highly specific for the PE product of the lyase reaction. (ed.ac.uk)
Bonds1
The lyase is highly specific toward α-1, 4-glucosidic bonds and shows negligible activity toward α-1, 6-glucosidic bonds. (go.jp)
Produces1
α-1, 4-Glucan lyase (EC 4.2.2.13) produces 1, 5-anhydro D-fructose from α-1, 4-linked glucose oligomers and polymers, such as maltose, maltosaccharides, starch and glycogen. (go.jp)
We also tested the lyase activity of the deoxyhyposyl-hydroxylase (DOHH) from the malaria parasite, Plasmodium falciparum. (uni-muenchen.de)
Strain1
Many of the most highly expressed hydrolases belonged to a Ca . Bathyarchaeota strain and may have been associated with recycling of bacterial biomass. (frontiersin.org)
Type2
Only two such lyases, which both belong to the E/F-type were known at the beginning of this work. (uni-muenchen.de)
Two additional types, S/(U)-type and T-type lyase, have been characterized during this work. (uni-muenchen.de)
Specifically1
more specifically the ox-acid-lyase or the carbon-carbon-lyases. (wikipedia.org)
Metal1
Another major difference to the pectate and other polysaccharide lyase mechanisms, is that divalent metal ions are not required by PL4 for catalysis. (cazypedia.org)
Products1
Nucleophilic addition products of PCB were characterized that are formed spontaneously or by the lyases, and gave first indications for a mechanistic model for the lyases. (uni-muenchen.de)