• receptor
  • Several investigators have reported that levels of SYK may correlate with basophil responsiveness and that SYK is degraded as a consequence of receptor-induced anergy. (openwetware.org)
  • However, OIT did not significantly suppress this response in basophils stimulated by the distinct fMLP receptor pathway. (nih.gov)
  • Here we investigated the cellular and molecular mechanisms underlying acquired resistance to Haemaphysalis longicornis ticks in mice and found that antibodies were required, as was IgFc receptor expression on basophils but not on mast cells. (pubmedcentralcanada.ca)
  • To assess the requirement for basophil infiltration to acquired tick resistance, mice expressing the human diphtheria toxin receptor under the control of the mast cell protease 8 (Mcpt8) promoter were generated. (pubmedcentralcanada.ca)
  • CD63
  • Basophil reactivity to in vitro stimulation with peanut allergen and controls was assessed by the upregulation of activation markers, CD63 and CD203c, measured by flow cytometry. (nih.gov)
  • Several minutes at room temperature gives the marker time to bind to the CD63 proteins on the cell membrane of the basophil. (wikipedia.org)
  • substantia
  • Small clusters of basophils were observed in the substantia propria of giant papillae, especially at the vicinity of vascular endothelium and subepithelial regions. (bmj.com)
  • allergen
  • This is the 2X basophil media to be used for incubation with the whole blood and for preparation of the egg or peanut allergen dilutions (see below). (openwetware.org)
  • To evaluate the hypothesis that basophil anergy occurs in vivo due to chronic allergen exposure in the setting of a clinical oral immunotherapy trial. (nih.gov)
  • tissue
  • CD4+ T cell, dendritic cell, B cell, mast cell, neutrophil, and basophil functions), they are involved in the destruction of tumor cells, and they promote the repair of damaged tissue. (wikipedia.org)
  • Although mast cells were once thought to be tissue resident basophils, it has been shown that the two cells develop from different hematopoietic lineages and thus cannot be the same cells. (wikipedia.org)
  • antibodies
  • Cryostat sections of giant papillae were immunostained with basophil-specific antibody BB-1, and with anti-CD4, anti-CD8, anti-CD20, anti-major basic protein (MBP), anti-IgE and anti-FcɛRI-β antibodies. (bmj.com)
  • blood
  • The occurrence of basophils in peripheral blood of 15 freshwater teleosts was examined using the metachromatic stain, toluidine blue, on blood smears fixed with lead subacetate. (ingentaconnect.com)
  • Basophil activation in unfractionated samples such as whole blood can be measured by changes in the expression of cell surface markers or even intracellular events (e.g. phosphorylation, oxidative burst, calcium flux) by flow cytometry. (openwetware.org)
  • New page: ==Overview== Basophil activation in unfractionated samples such as PBMC (note basophils are less dense the Ficoll) or whole blood can be measured by changes in the expression of cell surf. (openwetware.org)
  • Basophil activation in unfractionated samples such as PBMC (note basophils are less dense the Ficoll) or whole blood can be measured by changes in the expression of cell surface markers or even intracellular events (e.g. phosphorylation, oxidative burst, calcium flux) by flow cytometry. (openwetware.org)
  • Evidence
  • Recent evidence suggests that basophils are an important source of the cytokine, interleukin-4, perhaps more important than T cells. (wikipedia.org)
  • These data provide the first clear evidence, to our knowledge, that basophils play an essential and nonredundant role in antibody-mediated acquired immunity against ticks, which may suggest new strategies for controlling tick-borne diseases. (pubmedcentralcanada.ca)
  • chronic
  • Increase in basophil leucocytes (counts above 0.1 X 10^9/liters) is uncommon, but this is seen in myeloproliferative disorders such as chronic myeloid leukemia and polycythemia vera. (hubpages.com)