• Barbituric acid merocyanine (BAM) nucleosides in synthetic nucleic acid nanostructures show exciton coupling that can be tuned by the double helix conformation. (uni-wuerzburg.de)
  • Structural studies of the free nucleoside revealed that both bases are preferred syn conformation, thus mimicking the conformation of the oxopurine nucleosides. (elsevierpure.com)
  • LNA sequences are RNA analogues "locked" into an ideal Watson-Crick base pairing conformation. (wikipedia.org)
  • Cascade will release crRNAs that will bind with foreign nucleic acid sequences - via base pair matching to a "seed" sequence of nucleotides - and silence or otherwise inactivate them. (lbl.gov)
  • Incorporating Locked Nucleic Acid into oligonucleotides increases thermal duplex stability 2 and improves the specificity of oligonucleotide hybridization to target sequences. (sigmaaldrich.com)
  • The bold letters in the sequences denote Locked Nucleic Acid bases. (sigmaaldrich.com)
  • Specifically, Locked Nucleic Acid oligonucleotides can be designed to address traditionally problematic target sequences, such as AT- or GC-rich regions. (sigmaaldrich.com)
  • Secondary structure can be predicted from one or several nucleic acid sequences. (wikipedia.org)
  • basic concepts of sequence alignment: local and global alignments, Needleman and Wunsch, Smith and Waterman algorithms for pairwise alignments, gap penalties, use of pairwise alignments for analysis of Nucleic acid and protein sequences and interpretation of results Multiple sequence alignments (MSA): the need for MSA, basic concepts of various approaches for MSA (e.g. progressive, hierarchical etc. (entrance.net.in)
  • In protein synthesis, DNA is first transcribed into mRNA, and then translated in an amino acid sequence. (wikipedia.org)
  • CRISPRs are transcribed and the long primary transcript is processed into a library of short CRISPR-derived RNAs (crRNAs), each of which contains a unique sequence that is complementary to a foreign nucleic acid challenger. (lbl.gov)
  • In DNA a sequence of three bases, which is called a codon, is responsible for the encoding of a single amino acid. (wikibooks.org)
  • Nucleic acid structure prediction is a computational method to determine secondary and tertiary nucleic acid structure from its sequence. (wikipedia.org)
  • many molecules have several possible three-dimensional structures, so predicting these structures remains out of reach unless obvious sequence and functional similarity to a known class of nucleic acid molecules, such as transfer RNA (tRNA) or microRNA (miRNA), is observed. (wikipedia.org)
  • A common problem for researchers working with RNA is to determine the three-dimensional structure of the molecule given only a nucleic acid sequence. (wikipedia.org)
  • Influence of local sequence context on damaged base conformation in human DNA polymerase iota: molecular dynamics studies of nucleotide incorporation opposite a benzo[a]pyrene-derived adenine lesion. (neb.com)
  • When this conversion occurs in the coding region of mRNA, it results in an altered nucleotide codon and, therefore, can change the amino acid sequence of the coded protein in what is referred to as a re-coding editing event. (biomedcentral.com)
  • DNA nanotechnology is the design and manufacture of artificial nucleic acid structures for technological uses. (health-innovations.org)
  • Analyzing and building nucleic acid structures with 3DNA. (x3dna.org)
  • It is a part of the 3DNA suite [67] designed to work with the structures of nucleic acids. (x3dna.org)
  • Inspired by natural Watson-Crick base pairing and genetic code expansion by hydrophobic base pairing, we explore dipolar and quadrupolar stacking interactions as bioorthogonal elements for the assembly of supramolecular nucleic acid structures. (uni-wuerzburg.de)
  • Life is based on carbon compounds including carbohydrates, lipids, proteins and nucleic acids. (ivy-way.com)
  • In this field, nucleic acids are used as engineering materials for nanotechnology rather than as the carriers of genetic information in living cells. (health-innovations.org)
  • His research interests include structure-based design of functional nucleic acids useful in medicine and nanotechnology. (asiaresearchnews.com)
  • In addition, the improved hybridization of the PCR primer or qPCR probe to its target may increase the melting temperature (T m ) by up to 8 °C per Locked Nucleic Acid monomer substitution in medium salt conditions compared to native-state DNA oligonucleotides 4 ( Table 1 ). (sigmaaldrich.com)
  • Due to Locked Nucleic Acid's improved hybridization characteristics with accompanied increase in T m , Locked Nucleic Acid oligonucleotides can be synthesized to be shorter, which overcomes certain design limitations that occur with native-state DNA oligonucleotides. (sigmaaldrich.com)
  • He is the Professor of Nucleic acid chemistry at the Department of Chemistry [6] and Department of Oncology [7] at the University of Oxford . (wikimili.com)
  • [21] In 2013, Brown again moved, to take up position of the Professor of Nucleic acid chemistry at the University of Oxford where he now holds a joint position at the Department of Chemistry and Department of Oncology. (wikimili.com)
  • The mimics are typically composed of locked nucleic acids (LNA), 2'-OMe, or 2'-F modified bases. (wikipedia.org)
  • LNAs, 2'-OMe, or 2'-F modified bases are chemical analogs of natural RNA nucleic acids. (wikipedia.org)
  • Table 1 Increasing the number of Locked Nucleic Acid bases in an oligonucleotide increases the T m . (sigmaaldrich.com)
  • The ability of oligonucleotides to discriminate between alleles via SNPs is greatly enhanced by the incorporation of Locked Nucleic Acid bases 6-8 ( Figure 2 ). (sigmaaldrich.com)
  • Shorter oligonucleotides incorporating Locked Nucleic Acid bases can be used at the same temperatures as longer native-state DNA oligonucleotides. (sigmaaldrich.com)
  • Pink) mutant DNA analysis with Locked Nucleic Acid mutant probe (16mer with 3 Locked Nucleic Acid bases). (sigmaaldrich.com)
  • Purple) wild-type DNA with Locked Nucleic Acid mutant Probe (16mer with 3 Locked Nucleic Acid bases). (sigmaaldrich.com)
  • With Locked Nucleic Acid oligonucleotides, the selective placement of Locked Nucleic Acid bases facilitates the optimal design of highly-specific, shorter oligonucleotides that perform well, even at lengths of only 13 to 20 bases. (sigmaaldrich.com)
  • DNA is a long chain of linear polymers containing deoxyribose sugars and their covalently bonded bases known as nucleic acids. (wikibooks.org)
  • Oxidation of purine bases at the 8-position, yielding 8-oxoguanosine and 8-oxoadenosine, results in conformation changes that cause miscoding during DNA replication. (elsevierpure.com)
  • Locked Nucleic Acid in PCR primers, qPCR probes, and other types of oligonucleotides is soluble in water and standard buffers as well as follows Watson-Crick base-pairing rules. (sigmaaldrich.com)
  • Also, the design of oligonucleotides for targeting difficult SNPs, such as the relatively stable G:T mismatch, is greatly facilitated by Locked Nucleic Acid. (sigmaaldrich.com)
  • Each nanocluster is tightly wrapped and almost completely shielded by two DNA strands in a horseshoe conformation. (asiaresearchnews.com)
  • This site mentions the transient H-bonds formed by holoenzyme at promoter sites, but I haven't been ale to fnd a reputable source discussing the transient H-bonds between nucleic acid strands. (stackexchange.com)
  • The benzo[c]phenanthrenyl and nucleic acid exchangeable and nonexchangeable protons were assigned in the predominant conformation following analysis of two-dimensional NMR data sets in H2O and D2O buffer solution. (psu.edu)
  • A novel mechanism is unveiled to explain why a pro-mutagenic nucleotide lesion (oxidized guanine, 8-oxoG) causes the mammalian DNA repair polymerase-β (pol-β) to rapidly transition to an inactive open conformation. (nyu.edu)
  • The presence of a single base mismatch has a greater destabilizing effect on the duplex formation between an Locked Nucleic Acid oligonucleotide and its target than with a native-state DNA oligonucleotide. (sigmaaldrich.com)
  • and imaging and tracking systems, biosensor systems, and biomarker finding.7,16,17,18 Moreover, the nature of nucleic acid provides another bonus that the function of aptamers can be 176644-21-6 IC50 modulated for desired therapy and drug delivery applications using the complementary oligo antidote strategy. (scienceexhibitions.org)
  • Crespo-Hernández, C. E., "On the Photostability of Cyanuric Acid and its Candidature as a Prebiotic Nucleobase", Molecules 2022, 27(4), 1184. (case.edu)
  • As of January 2016, Brown has published more than 300 articles in peer-reviewed journals, with many of his papers appearing in highly selective journals like Nature , Nature Biotechnology , Cell , Nucleic Acids Research , JACS , and PNAS . (wikimili.com)
  • Over the past three decades, DNA has emerged as an exceptional molecular building block for nano construction due to its predictable conformation and programmable base-pairing interactions. (health-innovations.org)
  • article{, title = {Comprehensive features of natural and in vitro selected GNRA tetraloop-binding receptors}, author = { C. Geary and S. Baudrey and L. Jaeger}, year = {2008}, date = {2008-01-01}, journal = {Nucleic Acids Res}, volume = {36}, number = {4}, pages = {1138-52}, abstract = {Specific recognitions of GNRA tetraloops by small helical receptors are among the most widespread long-range packing interactions in large ribozymes. (cnrs.fr)
  • Although the structure of RNA is highly flexible, the underlying dynamics of RNA are robust and are limited to transitions between the few conformations that preserve favourable base-pairing and stacking interactions. (duke.edu)
  • This bridge-locked in the 3'-endo conformation-restricts the flexibility of the ribofuranose ring and locks the structure into a rigid bicyclic formation. (sigmaaldrich.com)
  • Changes to the conformation of coding and non-coding RNAs form the basis of elements of genetic regulation and provide an important source of complexity, which drives many of the fundamental processes of life. (duke.edu)
  • Nucleic acids convey genetic information. (spilianakislab.gr)
  • Nucleic acids research , 44 (9), 4409-4424. (nyu.edu)
  • Through a combination of cryo-electron microscopy and three-dimensional image reconstruction, they have determined the structure of a protein complex called "Cascade," that acts as a surveillance system for detecting and inactivating the nucleic acid of invading pathogens. (lbl.gov)
  • The amino acid is added to a growing protein during the process of translation. (wikibooks.org)
  • The energy parameters are also different for the two nucleic acids. (wikipedia.org)
  • Figure 1 illustrates results from analytical size-exclusion chromatography (SEC) of a commercial BSA preparation marketed for enzyme stabilization in conjunction with processing nucleic acids. (bioprocessintl.com)
  • DNA is a nucleic acid which is capable of duplicating itself via the enzyme known as DNA polymerase. (wikibooks.org)
  • Structure of Locked Nucleic Acid and native-state DNA monomers. (sigmaaldrich.com)
  • This article is about the computational prediction of nucleic acid structure. (wikipedia.org)
  • For experimental methods, see Nucleic acid structure determination . (wikipedia.org)
  • These Multiple Choice Questions (MCQs) should be practiced to improve the Nucleic Acid Structure skills required for various interviews (campus interview, walk-in interview, company interview), placement, entrance exam and other competitive examinations. (dapzoi.com)
  • of nucleic acid structure (Westhof, 1988). (lu.se)
  • Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. (lookformedical.com)
  • These nucleic acid polymers encode for the all of the materials an organism needs to live in the form of genes. (wikibooks.org)
  • Amino acids are linked together by condensation to form polypeptides. (ivy-way.com)
  • This movie shows the conformation of Cascade's subunits - CasA (purple), CasB (yellow) and CasE (magenta) - being rearranged while the helical spine of the complex remains relatively undisturbed. (lbl.gov)
  • The m(6)A-modified hairpin has a predisposed conformation that resembles the hairpin conformation in the RNA-HNRNPC complex more closely than the unmodified hairpin. (nih.gov)