• genomic
  • Combining sequencing data with Illumina PorcineSNP60 BeadChip genotyping results on the same animals, 661 genomic positions overlapped with a good approximation of minor allele frequency estimation. (hindawi.com)
  • Here we report on the sequencing, assembly, and analyses of 96 randomly chosen BACs from sunflower to provide additional information on the repeat content of the sunflower genome, assess how repetitive elements in the sunflower genome are organized relative to genes, and compare the genomic distribution of these repeats to that found in other food crops and model species. (mdpi.com)
  • Since genomic sequencing has so far only touched the surface of protist diversity, it is anticipated that sequences of additional protists may reveal an even wider range of metabolic capabilities, while simultaneously enriching our understanding of the early evolution of eukaryotes. (pubmedcentralcanada.ca)
  • NextCODE will leverage deCODE's Genomic Ordered Relational (GOR) database infrastructure and sequence analysis systems, and will continue to develop these systems for specific needs associated with clinical research and care. (bio-medicine.org)
  • Because cytosine / guanine base-pairing is generally stronger than adenine / thymine base-pairing, the amount of cytosine and guanine in a genome (called the "GC content") can be estimated by measuring the temperature at which the genomic DNA melts. (wikipedia.org)
  • Excision of gene coding sequences from genomic DNA. (wikipedia.org)
  • mutations
  • NextCODE's Clinical Sequence Analyzer (CSA) facilitates rapid confirmation of mutations through visualization of raw sequence data in real time, providing greater transparency than existing systems and enabling users to validate their findings with speed and confidence. (bio-medicine.org)
  • Access to whole cancer genome sequencing is important to cancer (or cancer genome) research because: Mutations are the immediate cause of cancer and define the tumor phenotype. (wikipedia.org)
  • The remaining 8 were new mutations and all were single base changes: Four were in families that are strongly associated with cancer pathogenesis (PTPRT, CDH24, PCLKC and SLC15A1). (wikipedia.org)
  • Conserved sequences, such as mitochondrial DNA, are expected to accumulate mutations over time, and assuming a constant rate of mutation, provides a molecular clock for dating divergence. (wikipedia.org)
  • Molecules
  • Method for rapid-base sequencing in DNA and RNA with two-base labeling and employing fluorescent detection of single molecules at two wavelengths. (patents.com)
  • High-Speed DNA Sequencing: An Approach Based Upon Fluorescence Detection of Single Molecules," J. Biomolecular Struc. (patents.com)
  • Strand separation by gentle heating, as used in PCR, is simple providing the molecules have fewer than about 10,000 base pairs (10 kilobase pairs, or 10 kbp). (wikipedia.org)
  • Hybrid molecules are formed between similar sequences and any differences between those sequences will result in a disruption of the base-pairing. (wikipedia.org)
  • A comprehensive step-by-step protocol on constructing phylogenetic tree, including DNA/Amino Acid contiguous sequence assembly, multiple sequence alignment, model-test (testing best-fitting substitution models) and phylogeny reconstruction using Maximum Likelihood and Bayesian Inference, is available at Nature Protocol Early attempts at molecular systematics were also termed as chemotaxonomy and made use of proteins, enzymes, carbohydrates, and other molecules that were separated and characterized using techniques such as chromatography. (wikipedia.org)
  • Morpholinos block access of other molecules to small (~25 base) specific sequences of the base-pairing surfaces of ribonucleic acid (RNA). (wikipedia.org)
  • Instead, Morpholinos act by "steric blocking", binding to a target sequence within an RNA, inhibiting molecules that might otherwise interact with the RNA. (wikipedia.org)
  • genome sequence
  • Our vision is to transform patient diagnosis and resultant care through the rapid and accurate use of genome sequence data, and we are deploying the most powerful tools ever developed to make this vision a reality," said Smarason. (bio-medicine.org)
  • In August 2011, the company expanded its recruiting process by offering an incentive of $20,000 plus a full genome sequence to employees who referred a software engineer. (wikipedia.org)
  • isolate
  • Screening of antibody libraries by phage display allows for rapid selection of single-chain variable fragments (scFvs), from which to isolate the sequences of variable heavy (VH) and variable light (VL) chains for mAb conversion. (hindawi.com)
  • homology
  • In 1999, working with John Carlson at Yale, he developed a novel algorithm for computationally identifying G Protein-Coupled Receptors (GPCR) without using primary sequence homology, which led to the cloning of olfactory receptors in insects. (wikipedia.org)
  • examined misidentification of VGS submitted to antimicrobial surveillance programs as pneumococci and found that the distinction of S. pneumoniae from the VGS can be difficult, which is not surprising in light of the fact that S. mitis and S. oralis possess >99% sequence homology with S. pneumoniae (47). (wikipedia.org)
  • Screening
  • To this aim, we implemented a high-throughput sequencing approach for library screening, followed by a simple and effective strategy to recover active binder clones from enriched sublibraries. (hindawi.com)
  • High-throughput sequencing (HTS) was successfully applied to phage display technology, to get full advantage from screening of phage display libraries [ 18 , 19 ]. (hindawi.com)
  • genetic
  • CAMBRIDGE, Mass. , Oct. 23, 2013 /PRNewswire/ -- NextCODE Health, a new private company enabling accelerated diagnosis of genetic diseases through the use of sequence data, announced today that it has secured an exclusive license for sequence-based clinical diagnostic applications using technology developed by deCODE genetics. (bio-medicine.org)
  • Analysis will be supported by access to deCODE's substantial, proprietary knowledge base that includes more than 40 million validated variants, representing the world's largest collection of genetic variants paired with clinical data. (bio-medicine.org)
  • In general, these are considered superior for evolutionary studies, since the actions of evolution are ultimately reflected in the genetic sequences. (wikipedia.org)
  • restriction
  • citation needed] While a postdoctoral fellow with Hamilton O. Smith at the Johns Hopkins University School of Medicine during 1969-70, Kelly determined the DNA sequences recognized by type II restriction enzymes, which subsequently became major tools in recombinant DNA research. (wikipedia.org)
  • The underlying assumption is that each microbe in the sample will have a different sequence on the target gene, so a restriction enzyme will cut each microbe's DNA in a different place. (wikipedia.org)
  • detect
  • DNA denaturation can also be used to detect sequence differences between two different DNA sequences. (wikipedia.org)
  • In the context of a single isolated region of DNA, denaturing gradient gels and temperature gradient gels can be used to detect the presence of small mismatches between two sequences, a process known as temperature gradient gel electrophoresis. (wikipedia.org)
  • canonical
  • Evident from genome sequencing are examples of versatile metabolism that go far beyond the canonical pathways described for eukaryotes in textbooks. (pubmedcentralcanada.ca)
  • In the canonical Watson-Crick base pairing, adenine (A) forms a base pair with thymine (T) and guanine (G) forms one with cytosine (C) in DNA. (wikipedia.org)
  • Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix). (wikipedia.org)
  • The viral polymerase incorporates these compounds with non-canonical bases. (wikipedia.org)
  • These nucleoside triphosphates possess a non-canonical sugar, dideoxyribose, which lacks the 3' hydroxyl group normally present in DNA and therefore cannot bond with the next base. (wikipedia.org)
  • Genomics
  • NextCODE Health Launches Operations with Exclusive License to Leverage deCODE genetics' Genomics Platform for Sequence-Based Clinical Diagnostics, and $15 Million in Venture. (bio-medicine.org)
  • Genomics data generated by next generation sequencing technologies have become increasingly important to many aspects of healthcare, from diagnosing disease to defining patients' risk for specific illnesses and selecting the most effective treatments for an individual patient. (bio-medicine.org)
  • Cloud-Based Genomics. (wikipedia.org)
  • http://www.the-scientist.com/?articles.view/articleNo/38044/title/Cloud-Based-Genomics/ Thomas, Uduak Grace. (wikipedia.org)
  • Gene Expression
  • For addressing the needs of modeling, simulation and visualization of large and diverse biological datasets from sequence, gene expression and proteomics datasets, "systems biology" (Hood 2003) approaches are being developed for construction of gene regulatory networks (Dojer et al. (springer.com)
  • wobble
  • In RNA, thymine is replaced by uracil (U). Alternate hydrogen bonding patterns, such as the wobble base pair and Hoogsteen base pair, also occur-particularly in RNA-giving rise to complex and functional tertiary structures. (wikipedia.org)
  • The GU wobble base pair, with two hydrogen bonds, does occur fairly often in RNA. (wikipedia.org)
  • The first anticodon base, or wobble-position, is sometimes modified to inosine (derived from adenine), pseudouridine or lysidine (derived from cytosine). (wikipedia.org)
  • Some anticodons can pair with more than one codon due to a phenomenon known as wobble base pairing. (wikipedia.org)
  • analysis
  • we would like to do some sequence analysis with the students. (bioinformatics.org)
  • DNAnexus is an American company that provides a cloud-based data analysis and management platform for DNA sequence data. (wikipedia.org)
  • DNAnexus was founded in early 2009 as a spin-off from Stanford University to address the need for computing infrastructure in DNA sequence analysis. (wikipedia.org)
  • Customers of DNAnexus use those computational resources to run analysis programs on DNA sequence data and to store that data. (wikipedia.org)
  • DNAnexus Aims to Alleviate Next-Gen Sequence Analysis Headache with Cloud-based Service. (wikipedia.org)
  • http://www.genomeweb.com/sequencing/dnanexus-aims-alleviate-next-gen-sequence-analysis-headache-cloud-based-service Ricketts, Camille. (wikipedia.org)
  • http://www.fiercebiotechit.com/special-reports/fierce-s-top-10-biotech-techies/andreas-sundquist-fierces-top-10-biotech-techies http://genengnews.com/gen-news-highlights/baylor-39-s-hgsc-dnanexus-amazon-team-up-in-cloud-based-analysis/81249025/?kwrd=dnanexus Grens, Kerry. (wikipedia.org)
  • strands
  • DNA denaturation, also called DNA melting, is the process by which double-stranded deoxyribonucleic acid unwinds and separates into single-stranded strands through the breaking of hydrophobic stacking attractions between the bases. (wikipedia.org)
  • cleavage
  • The bases are then sequentially cleaved from the replicated strand, excited with a chosen spectrum of electromagnetic radiation, and the fluorescence from individual, tagged bases detected in the order of cleavage from the strand. (patents.com)
  • EcoRII is a bacterial Type IIE REase that interacts with two or three copies of the pseudopalindromic DNA recognition sequence 5'-CCWGG-3' (W = A or T), one being the actual target of cleavage, the other(s) serving as the allosteric activator(s). (wikipedia.org)
  • typical
  • This study sequenced a typical acute myeloid leukaemia (AML) genome and its normal counterpart genome obtained from the same patient. (wikipedia.org)
  • they also lack either the polysaccharide-based capsule typical of S. pneumoniae or the Lancefield antigens of the pyogenic members of the genus. (wikipedia.org)
  • identify
  • In step two a refined version of the 'daylight feasibility study' is used to help the design team to identify building zones with high daylighting potential based on a target mean daylight factor criterion. (epfl.ch)
  • Stem
  • The T arm is a 4- to 5- bp stem containing the sequence TΨC where Ψ is pseudouridine, a modified uridine. (wikipedia.org)
  • examples
  • In the last 2-3 years, genome sequencing and transcript profiling has unveiled several examples of heterotrophic and phototrophic protists that are unexpectedly well-equipped for ATP production using a facultative anaerobic metabolism, including some protists that can ( Chlamydomonas reinhardtii ) or are predicted ( Naegleria gruberi , Acanthamoeba castellanii , Amoebidium parasiticum ) to produce H 2 in their metabolism. (pubmedcentralcanada.ca)
  • different
  • The secondary structures of biological DNA's and RNA's tend to be different: biological DNA mostly exists as fully base paired double helices, while biological RNA is single stranded and often forms complex and intricate base-pairing interactions due to its increased ability to form hydrogen bonds stemming from the extra hydroxyl group in the ribose sugar. (wikipedia.org)
  • Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. (wikipedia.org)
  • specific
  • A modified S-SAP (sequence-specific amplified polymorphism) method with universal primers for retrotransposons was used to study Vitis vinifera cv. (ajevonline.org)
  • single
  • Bases modified to accept fluorescent labels are used to replicate a single DNA or RNA strand to be sequenced. (patents.com)
  • a substantial sample of individuals of the target species or other taxon is used, however many current studies are based on single individuals. (wikipedia.org)
  • steps
  • All steps within the sequence were 'validated' using Radiance simulations of over 2300 sidelit spaces. (epfl.ch)
  • Particular base steps are also susceptible to DNA melting, particularly T A and T G base steps. (wikipedia.org)