• NCTC
  • The collagen binding proteins isolated by both these independent methods were subjected to tandem mass spectroscopy for peptide identification and matched to a single hypothetical protein encoded by B. fragilis NCTC 9343 (BF0586), conserved in YCH46 (BF0662) and 638R (BF0633) and which is designated in this study as cbp1 (collagen binding protein). (nrf.ac.za)
  • gene
  • The major determinant of carbapenem resistance in Bacteroides fragilis is production of CfiA metallo-beta-lactamase via activation of the cfiA gene by IS elements (higher level resistance) or by activation of its putative own promoter. (diva-portal.org)
  • none of those with inactive disease had specimens positive for enterotoxigenic Bacteroides fragilis gene sequences. (cdc.gov)
  • Functionality of the protein was confirmed by targeted insertional mutagenesis of the cbp1 gene in B. fragilis GSH18 which resulted in the specific loss of both the ,31 kDa and the ,34 kDa adhesin bands. (nrf.ac.za)
  • Purified his-tagged Cbp1, expressed in a B. fragilis wild-type and a glycosylation deficient mutant, confirmed that the cbp1 gene encoded the observed collagen adhesin, and showed that the 34 kDa band represents a glycosylated version of the ,31 kDa protein. (nrf.ac.za)
  • This study is the first to report the presence of collagen type I adhesin proteins in B. fragilis and to functionally identify a gene encoding a collagen binding protein. (nrf.ac.za)
  • resistance
  • Production of beta lactamase appears to be the main mechanism of antibiotic resistance in B. fragilis. (wikipedia.org)
  • Clindamycin is no longer recommended as the first-line agent for B. fragilis due to emerging high-level resistance (>30% in some reports). (wikipedia.org)
  • intra-abdominal
  • B fragilis bacteraemia of unknown source should caution the physician to search for an intra-abdominal focus, such as thrombosis of the portal vein or mesenteric vein. (bmj.com)
  • susceptible
  • In general, B. fragilis is susceptible to metronidazole, carbapenems, tigecycline, beta-lactam/beta-lactamase inhibitor combinations (e.g. (wikipedia.org)
  • Protein
  • Collagen Far Western analysis of the B. fragilis outer membrane protein (OMP) fraction revealed the presence two collagen adhesin bands of ,31 and ,34 kDa. (nrf.ac.za)
  • below
  • B. fragilis has high protease and peptidase activities ( ) , which in turn provides the substrates for further nitrogen assimilatory enzymes, described below. (salamanderthemes.net)