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  • interactions
  • MIT researchers have discovered why an unusually short bacterial protein can have many more interactions than would normally be expected of something its size. (phys.org)
  • These crystal structures unravel a complex choreography of protein-protein interactions that will aid in the design of new antibacterial drugs," said Huilin Li, a biophysicist at the U.S. Department of Energy's Brookhaven National Laboratory and a professor at Stony Brook University, who participated in the research with a number of collaborators in the U.S. and in Europe. (phys.org)
  • Attachment further allows more interactions and increase of biofilm formation to aid bacterial colonization. (wikipedia.org)
  • The role of single-stranded DNA binding (SSB) protein during DNA replication in Escherichia coli cells has been studied, specifically the interactions between SSB and the χ subunit of DNA polymerase III in environments of varying salt concentrations. (wikipedia.org)
  • pathogens
  • Recent studies have revealed that viruses and bacterial pathogens exploit the host ubiquitination pathways to gain entry and to aid their survival/replication inside host cells. (frontiersin.org)
  • This review will summarize recent developments in understanding the biochemical and structural mechanisms utilized by bacterial pathogens to interact with the host ubiquitination pathways. (frontiersin.org)
  • structural
  • However, the information on preferable 3D structural motifs is available mostly for Ca 2+ and Zn 2+ binding proteins. (hindawi.com)
  • in these processes, bacterial DNA binding proteins have an architectural role, maintaining structural integrity as transcription, recombination, replication, or any other DNA-dependent process proceeds. (wikipedia.org)
  • polymerase
  • The research, conducted by a Rockefeller team led by Elizabeth Campbell, in collaboration with scientists at Memorial Sloan Kettering, focuses on a cluster of interacting proteins called RNA polymerase. (nanowerk.com)
  • the protein is involved in stabilizing the lagging strand as well as interacting with DNA polymerase III. (wikipedia.org)
  • In DNA replication at the lagging strand site, DNA polymerase III removes nucleotides individually from the DNA binding protein. (wikipedia.org)
  • interact
  • A protein like UmuD, which is made of fewer amino acids, would not be expected to have enough binding sites to interact with very many other proteins. (phys.org)
  • UmuD's ability to interact with multiple partner proteins allows it to control the function of two translesion polymerases, coordinating their action with DNA replication. (phys.org)
  • transporter
  • At the European Synchrotron Radiation Facility in Grenoble, France, the Washington University/UK group determined the atomic-level crystal structure of the entire transporter protein, known as an "usher," bound to the sticky adhesin subunit that forms the end of a pilus and another helper protein, called a chaperone, that shuttles the pilus subunits to the usher one at a time. (phys.org)
  • mechanism
  • This thesis addresses different aspects of the question about accuracy of protein synthesis: i) the mechanism of tRNA selection during translation ii) study of ribosomal mutations that affect accuracy and iii) the choice of aminoacyl-tRNA isoacceptors on synonymous codons. (diva-portal.org)
  • genes
  • When they injected Cb- -Rho into E. coli to examine the protein's function, they found that the protein misfolded in a prion-like manner, rendering it nonfunctional and allowing genes normally suppressed by Rho to be expressed. (the-scientist.com)
  • Crucial to all cells, this protein machine carries out a fundamental process in which genes within the DNA blueprint are copied into RNA. (nanowerk.com)
  • Another shared trait of bikonts is the fusion of two genes into a single unit: the genes for thymidylate synthase (TS) and dihydrofolate reductase (DHFR) encode a single protein with two functions. (wikipedia.org)
  • yeast
  • Martin and graduate student Robert Abramovitch previously found that AvrPtoB, a protein Pseudomonas injects into plants, disables PCD in a variety of susceptible plants and in yeast (a single-celled ancestor of both plants and animals). (innovations-report.com)
  • closely related
  • The integration host factor (IHF), a dimer of closely related chains which is suggested to function in genetic recombination as well as in translational and transcriptional control is found in Enterobacteria and viral proteins including the African swine fever virus protein A104R (or LMW5-AR). (wikipedia.org)
  • found
  • The team, led by biology professor Graham Walker, found that the protein, UmuD, belongs to a recently discovered class of proteins called intrinsically disordered proteins. (phys.org)
  • UmuD usually is found in groups of two, which implies that it must have some kind of stable protein structure, said Sharotka Maria Simon, lead author of the paper and an MIT Ph.D. recipient now at Brandeis University. (phys.org)
  • A protein from the bacterium, found in the aphid saliva and likely delivered inside the plant host by the aphid, triggers plant immune responses against the aphid. (redorbit.com)
  • One of these Buchnera proteins, GroEL, was found to induce immune responses in plants. (redorbit.com)
  • HU-type proteins have been found in a variety of eubacteria, cyanobacteria and archaebacteria, and are also encoded in the chloroplast genome of some algae. (wikipedia.org)
  • TSST-1 is a bacterial exotoxin found in patients who have developed toxic shock syndrome (TSS), which can be found in menstruating women or any man or child for that matter. (wikipedia.org)
  • transcription
  • These (bacterial proteins) function as a molecular pair of scissors, cutting up NF-kappaB transcription factors and thereby sabotaging the infected cells' immune response," said Teresa Thurston, the investigator at Imperial who oversaw the work. (eurekalert.org)
  • cells
  • Like a wolf in sheep s clothing, a protein from a disease-causing bacterium slips into plant cells and imitates a key host protein in order to cripple the plant s defenses. (innovations-report.com)
  • The YadA protein domain adheres to the following substrates: epithelial cells extracellular matrix collagen cellular fibronectin laminin The C-terminal domain consists of 120 amino acids which belong to a family of surface-exposed bacterial proteins. (wikipedia.org)
  • researchers
  • For this, the researchers need to integrate the individual components - which include bacterial proteins, dyes, and aptamers - into a sensor chip. (ecnmag.com)
  • To identify the protein composition of the aphid saliva, the researchers collected saliva from more than 100,000 aphids. (redorbit.com)
  • synthesis
  • that during translation the ribosomal A site is not blocked by unspecific binding of the non-cognate tRNAs which would inhibit the speed of protein synthesis. (diva-portal.org)
  • In the living cell the availability of cognate tRNAs versus the demand for them (the frequency of codon usage) is finely balanced to ensure critical protein synthesis in stress conditions. (diva-portal.org)
  • inactive
  • These changes rendered Pseudomonas harmless to susceptible tomato plants, and made the purified protein inactive. (innovations-report.com)
  • Meanwhile, Li's group worked at the National Synchrotron Light Source at Brookhaven to produce new images of the unbound usher protein in its closed, inactive state. (phys.org)
  • EspG functions as a Rab GTPase-activating protein (Rab-GAP), trapping Rab-GTPases in their inactive GDP bound form, and reducing ER-Golgi transport (of IL-8 and other proteins). (wikipedia.org)
  • lipids
  • The three main purposes of metabolism are the conversion of food/fuel to energy to run cellular processes, the conversion of food/fuel to building blocks for proteins, lipids, nucleic acids, and some carbohydrates, and the elimination of nitrogenous wastes. (wikipedia.org)
  • All Archaea have lipids with ether links between the head group and side chains, making the lipids more resistant to heat and acidity than bacterial and eukaryotic ester-linked lipids. (wikipedia.org)
  • gene
  • The EUBREC_0645 gene translates into the YP_002936568 protein from Eubacterium rectale , the most abundant member of Firmicutes phyla in the human gut, and it is the first solved representative of the bacterial TIR-like domain family (DUF1863/PF08937, e-value=0.00017). (burnham.org)
  • In this technique, a gene encoding a protein of interest is inserted into a phage coat protein gene, causing the phage to "display" the protein on its outside while containing the gene for the protein on its inside, resulting in a connection between genotype and phenotype. (wikipedia.org)
  • The phage gene and insert DNA hybrid is then inserted (a process known as "transduction") into Escherichia coli (E. coli) bacterial cells such as TG1, SS320, ER2738, or XL1-Blue E. coli. (wikipedia.org)
  • A few other mechanisms in E. coli that variously regulate initiation are DDAH (datA-Dependent DnaA Hydrolysis, which is also regulated by IHF), inhibition of the dnaA gene (by the SeqA protein), and reactivation of DnaA by the lipid membrane. (wikipedia.org)
  • This gene (dmdA) codes for a protein (DmdA) that removes a methyl group (-CH3) from DMSP. (wikipedia.org)
  • The DmdA protein has since been further characterized from S. pomeroyi, as well as the transcriptional response of the dmdA gene to the presence of DMSP and the sequence diversity of the dmdA gene. (wikipedia.org)
  • molecules
  • These displaying phages can then be screened against other proteins, peptides or DNA sequences, in order to detect interaction between the displayed protein and those other molecules. (wikipedia.org)
  • IFNs belong to the large class of proteins known as cytokines, molecules used for communication between cells to trigger the protective defenses of the immune system that help eradicate pathogens. (wikipedia.org)
  • The IncP-1 plasmid group (IncP plasmids in Escherichia coli) of which RK2 is a part has been described as "highly potent, self-transmissible, selfish DNA molecules with a complicated regulatory circuit" RK 2 was first isolated in connection with an outbreak of antibotic-resistant Pseudomonas aeruginosa and Klebsiella aerogenes in Birmingham in 1969, as one of a family of plasmids implicated in transfer of Ampicillin resistance between bacterial strains. (wikipedia.org)
  • binds
  • By immobilizing a relevant DNA or protein target(s) to the surface of a microtiter plate well, a phage that displays a protein that binds to one of those targets on its surface will remain while others are removed by washing. (wikipedia.org)
  • coli
  • If a "phagemid" vector is used (a simplified display construct vector) phage particles will not be released from the E. coli cells until they are infected with helper phage, which enables packaging of the phage DNA and assembly of the mature virions with the relevant protein fragment as part of their outer coat on either the minor (pIII) or major (pVIII) coat protein. (wikipedia.org)
  • host
  • Phage eluted in the final step can be used to infect a suitable bacterial host, from which the phagemids can be collected and the relevant DNA sequence excised and sequenced to identify the relevant, interacting proteins or protein fragments. (wikipedia.org)
  • respectively
  • for example, some steroids can beassayed by a radioimmunoassay, competitive protein-binding, gas-liquid chromatography, or indirectly where can i buy doxycycline in singapore by a 17-hydroxycor-ticosteroid assay.figures 5-16 and 5-17 show the results of a comparisonof prednisone tablets using a competitive protein-bindingmethod and a radioimmunoassay, respectively. (clubektmportugal.com)
  • vitamin
  • Poor nutrition makes wounds take longer to heal, according to Drugs.com, which states that the most important nutrients for wound healing are protein, zinc and vitamin C. To ensure optimum wound healing, you should consume the recommended level of vitamin C and zinc through food sources. (livestrong.com)
  • domain
  • Interestingly, 3hyn is structurally most similar to nucleotide binding proteins, not to the TIR domain (DALI top hit, 3ehd, belongs to the nucleoside 2-deoxyribosyl transferase family PF05014). (burnham.org)
  • The function of this protein domain is to digest DNA. (wikipedia.org)
  • include
  • Applications of phage display technology include determination of interaction partners of a protein (which would be used as the immobilised phage "bait" with a DNA library consisting of all coding sequences of a cell, tissue or organism) so that the function or the mechanism of the function of that protein may be determined. (wikipedia.org)
  • highly
  • These highly acidic proteins are overwhelmingly negative in charge and are able to remain in solution even at high salt concentrations. (wikipedia.org)
  • forms
  • The protein forms an oligomer and functions as an alkaline exonuclease, or in simpler terms, an enzyme that digests double-stranded DNA. (wikipedia.org)
  • Production
  • An increase in copy number is useful for genetic engineering applications to increase the production yield of recombinant protein. (wikipedia.org)
  • helps
  • Epsom salt is strongly hydroscopic and together with the other rock salt properties, cicatrizant, anti-inflammatory and anti-bacterial, helps to heal the wounds and relieve the itching. (dreampower.info)
  • Protein contributes to auto repair of the body, it is the best source to give energy and helps to build muscles. (longroadmedia.com)
  • large
  • In this way, large libraries of proteins can be screened and amplified in a process called in vitro selection, which is analogous to natural selection. (wikipedia.org)
  • changes
  • And recent reports from experiments in mouse models engineered to have Alzheimer's-like changes and symptoms suggest that changing the bacterial profile in their digestive tract-by changing their diet-may reduce amyloid plaques, lower inflammation and improve memory. (medicalxpress.com)
  • Study
  • Study done using fluorescence spectroscopy displayed a decrease in fluorescence emission of the protein upon binding to PLGA-COOH. (scirp.org)
  • further
  • This technology was further developed and improved by groups at the Laboratory of Molecular Biology with Greg Winter and John McCafferty, The Scripps Research Institute with Lerner and Barbas and the German Cancer Research Center with Breitling and Dübel for display of proteins such as antibodies for therapeutic protein engineering. (wikipedia.org)
  • human
  • It is therefore intriguing to find such protein in a dominant human symbiont. (burnham.org)
  • We investigated the interaction of PEGylated and un-PEGylated PLGA NPs with Recombinant Human Mannose-Binding Protein (HMBP) in an effort to understand the effect of surface modification on their binding to the protein. (scirp.org)
  • Gluten contains an amount of protein content that is recommended for the daily intake of a human. (longroadmedia.com)