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  • microbiology
  • Andrea is a Senior Staff Scientist based in Tennessee focused on leading-edge research using an array of geochemical, microbial, computational, and genomics techniques within the areas of environmental microbiology, computational biology, and engineering science. (geosyntec.com)
  • Type 1 pili-mediated adherence of Escherichia coli strain LF82 isolated from Crohn's disease is involved in bacterial invasion of intestinalepithelial cells, Boudeau, et al, Molecular Microbiology (2001) 39(5), pp 1272-1284. (patentgenius.com)
  • In microbiology, the term isolation refers to the separation of a strain from a natural, mixed population of living microbes, as present in the environment, for example in water or soil flora, or from living beings with skin flora, oral flora or gut flora, in order to identify the microbe(s) of interest. (wikipedia.org)
  • diversity
  • Andrea Rocha, Ph.D. (Tennessee) co-authored an article entitled "Microbial Functional Gene Diversity Predicts Groundwater Contamination and Ecosystem Functioning" published in mBio Volume 9, Issue 1 on February 20, 2018. (geosyntec.com)
  • The composition of this microbiota can now be rapidly assessed by a choice of methods depending on the question and the answers that can link our microbial diversity to health, diet, geographic location, disease and genotype. (wiley.com)
  • The PCR methods have been used with samples from sediments and microbial mats, as well as deep-sea holothurian guts, often showing unexpected bacterial and archaeal diversity. (asmscience.org)
  • Similar to exploration of bacterial diversity, these techniques have allowed high-resolution studies of fundamental questions in fungal ecology such as phylogeography, fungal diversity in forest soils, stratification of fungal communities in soil horizons, and fungal succession on decomposing plant litter. (wikipedia.org)
  • organism
  • The inability of Mycobacterium leprae to grow on axenic media has necessitated specialized techniques in order to determine viability of this organism. (nih.gov)
  • If one expects or looks for a particularly fastidious organism, the microbiological culture and isolation techniques will have to be geared towards that microbe. (wikipedia.org)
  • protein
  • Bacterial inclusion bodies are submicron protein clusters usually found in recombinant bacteria that have been traditionally considered as undesirable products from protein production processes. (biomedcentral.com)
  • Using an appropriate combination of chemical and mechanical cell disruption methods we have established a convenient procedure for the recovery of bacterial inclusion bodies with undetectable levels of viable cell contamination, below 10 -1 cfu/ml, keeping the particulate organization of these aggregates regarding size and protein folding features. (biomedcentral.com)
  • Listeria uses the cellular machinery to move around inside the host cell: it induces directed polymerization of actin by the ActA transmembrane protein , thus pushing the bacterial cell around. (thefullwiki.org)
  • It induces directed polymerization of actin by the ActA transmembrane protein, thus pushing the bacterial cell around. (wikipedia.org)
  • infections
  • In CF, acute polymicrobial bacterial infections overlap with chronic polymicrobial bacterial colonisation and thus findings from the CFMATTERS study have potential applications in other diseases involving Pseudomonas aeruginosa, and/or polymicrobial infection (both acute and chronic) such as wounds, burns, mechanical devices patients and ventilator associated pneumonias. (europa.eu)
  • CFMATTERS may potentially revolutionize the practice of antibiotic prescription in acute and chronic infections and limit the development of antimicrobial resistance globally by decreasing antibiotic consumption therefore decreasing the likelihood of bacterial resistance and side- effects from repeated non-personalized antibiotic courses. (europa.eu)
  • Meningitis is often complicated by encephalitis, a pathology that is unusual for bacterial infections. (thefullwiki.org)
  • Since the cell wall is required for bacterial survival, but is absent in some eukaryotes, several antibiotics (notably the penicillins and cephalosporins) stop bacterial infections by interfering with cell wall synthesis, while having no effects on human cells which have no cell wall only a cell membrane. (wikipedia.org)
  • microbes
  • The oldest method for obtaining information on microbial community structure is to examine the sample with a microscope and characterize the microbes by their morphology. (asmscience.org)
  • Depending on the expected density and viability of microbes present in a liquid sample, physical methods to increase the gradient as for example serial dilution or centrifugation may be chosen. (wikipedia.org)
  • genes
  • 8 The consequences of disturbances in DWDS are the emergence of microbial agents associated with waterborne diseases, 9 changes in ecosystem processes ( i.e. , microbial functional capabilities), 6 and the occurrence and dissemination of antibiotic resistance genes (ARGs). (rsc.org)
  • mats
  • This study underscores the presence of diverse mechanisms of adaptation to cold and other stresses in polar mats, consistent with the proportional representation of major bacterial groups. (pubmedcentralcanada.ca)
  • Microbial mats dominated by cyanobacteria are commonly found in extreme environments, such as geothermal springs, hypersaline basins, ultraoligotrophic ponds, and hot and cold desert soils ( 10 , 14 ). (pubmedcentralcanada.ca)
  • detection
  • Bacilli were harvested on day 1 and at 4, 8, 12, and 17 weeks post infection and enumerated by direct counting (A). Viability of M. leprae was determined by BacLight viability staining (B) and RR (C). Dotted line represents the lower detection limit of the assay. (nih.gov)
  • soil
  • In order to isolate organisms in materials with high microbial content, such as sewage, soil or stool, serial dilutions will increase the chance of separating a mixture. (wikipedia.org)
  • infection
  • CFMATTERS offers a stratified next generation approach to antibacterial treatment in a disease model of chronic bacterial infection with super-imposed acute exacerbations. (europa.eu)
  • Given the difficulties associated with assessment of response to antibiotic treatment the development of such a biomarker(s) would be a welcome advance for patients with acute and/or chronic microbial infection. (europa.eu)
  • communities
  • We anticipate that this approach can be expanded to other environments to enhance isolation and characterization of diverse microbial communities. (geosyntec.com)
  • A semi-closed pipe-loop chloraminated drinking water distribution system (DWDS) simulator was used to evaluate the biological stability of the system and describe the response of microbial communities in the bulk water (BW) and biofilm (BF) phase to a disturbance caused by changes in the operational parameters. (rsc.org)
  • Understanding changes in microbial communities in disturbed drinking water distribution systems is essential for monitoring biological stability and for proper risk management to ensure public and ecosystem health. (rsc.org)
  • Nitrification and chlorine burns in a DWDS are considered disturbances to microbial communities, because they alter the immediate environment by changing the physico-chemical conditions of the system. (rsc.org)
  • The microbial world within us includes a vast array of gastrointestinal (GI) tract communities that play an important role in health and disease. (wiley.com)
  • molecular
  • Molecular assays for determining Mycobacterium leprae viability in tissues of experimentally infected mice. (nih.gov)
  • The purpose of this study was to develop a simple and sensitive molecular assay for determining M. leprae viability directly from infected tissues. (nih.gov)
  • Molecular enumeration (RLEP PCR) and viability determinations (qRT-PCR) were performed via Taqman methodology on DNA and RNA, respectively, purified from ethanol-fixed FP tissue and compared with conventional enumeration (microscopic counting of acid fast bacilli) and viability assays (radiorespirometry, viability staining) which utilized bacilli freshly harvested from the contralateral FP. (nih.gov)
  • hsp18 and esxA qRT-PCR are sensitive molecular indicators, reliably detecting viability of M. leprae in tissues without the need for bacterial isolation or immediate processing, making these assays applicable for in vivo drug screening and promising for clinical and field applications. (nih.gov)
  • conventional
  • Unfortunately, the majority of strains in Sarawak, Borneo, are susceptible to aminoglycosides and macrolides, which means the conventional recommendations for isolation and identification do not apply there. (wikipedia.org)
  • mechanisms
  • Whilst many specific social immune mechanisms have been studied for some time in relative isolation (e.g. the "collective medication" found in wood ants), it was only until Sylvia Cremer et al. (wikipedia.org)
  • techniques
  • Historically, the laboratory techniques of isolation first developed in the field of bacteriology and parasitology (during the 19th century), before those in virology during the 20th century. (wikipedia.org)
  • Proper isolation techniques of virology did not exist prior to the 20th century. (wikipedia.org)
  • cell
  • The application of the developed protocol allows obtaining bacterial free inclusion bodies suitable for use in mammalian cell cultures and other biological interfaces. (biomedcentral.com)
  • Here, we demonstrate that Foxp3 + T reg cell deficiency results in gut microbial dysbiosis and autoimmunity over the lifespan of scurfy (SF) mouse. (rupress.org)
  • About half of the dry mass of a bacterial cell consists of carbon, and also about half of it can be attributed to proteins. (wikipedia.org)
  • As in other organisms, the bacterial cell wall provides structural integrity to the cell. (wikipedia.org)
  • The bacterial cell wall differs from that of all other organisms by the presence of peptidoglycan which is located immediately outside of the cytoplasmic membrane. (wikipedia.org)
  • Peptidoglycan is responsible for the rigidity of the bacterial cell wall and for the determination of cell shape. (wikipedia.org)
  • While all bacterial cell walls (with a few exceptions e.g. extracellular parasites such as Mycoplasma) contain peptidoglycan, not all cell walls have the same overall structures. (wikipedia.org)
  • If the bacterial cell wall is entirely removed, it is called a protoplast while if it's partially removed, it is called a spheroplast. (wikipedia.org)
  • β-Lactam antibiotics such as penicillin inhibit the formation of peptidoglycan cross-links in the bacterial cell wall. (wikipedia.org)
  • methods
  • Bacterial lysis is achieved using either mechanical or non mechanical methods, or a combination of both, while washing steps include, among others, detergent and/or DNase treatments. (biomedcentral.com)
  • Methods of microbial isolation have drastically changed over the past 50 years, from a labor perspective with increasing mechanization, and in regard to the technology involved, and hence speed and accuracy. (wikipedia.org)
  • analysis
  • This type of analysis is often used to check the viability of cells and performed with pinners (often also called froggers). (wikipedia.org)
  • This bacterial system might also be able to provide some utility for the analysis of eukaryotic Mg2+ transport proteins, but differences in biological systems of prokaryotes and eukaryotes will have to be considered in any experiment. (wikipedia.org)
  • products
  • This turn in the understanding of IB biological nature has prompted to explore potential applications of such aggregates as straightforward bacterial products. (biomedcentral.com)
  • community structure
  • These results provide evidence of variations in the bulk water/biofilm microbial community structure during episodes of disturbance ( e.g. , disinfectant switching practices, nitrification) and its recovery after disturbance. (rsc.org)
  • resistance
  • An estimated 25,000 patients die annually and approximately €0.9 billion is spent on additional health costs related to bacterial resistance. (europa.eu)
  • Treatment
  • In contrast, viability was markedly decreased by 8 weeks in immunocompetent mice.Rifapentine significantly reduced bacterial viability after 5 treatments, whereas rifampin required up to 20 treatments for the same efficacy.Neither drug was effective after a single treatment. (nih.gov)
  • In addition, the ability of these assays to detect the effects of anti-leprosy drug treatment on M. leprae viability was determined using rifampin and rifapentine, each at 10 mg/kg for 1, 5, or 20 daily doses, in the athymic nu/nu FP model. (nih.gov)
  • results
  • These results demonstrate the viability and utility of using exometabolomics to construct a tractable environmentally relevant media. (geosyntec.com)
  • water
  • Both are dried gradually on the substrate, ensuring their viability for anticipated reactivation when the Jiuqu is added to a new source of water and nutrition. (wikipedia.org)
  • recovery
  • This research provides evidence of the recovery (resilience) of the microbial community after episodes of disturbance (nitrification, disinfectant switching practices). (rsc.org)
  • relative
  • 4 In general, the outcome of a disturbance may be: (i) complete removal of the microbial community or (ii) changes in their relative abundances. (rsc.org)
  • changes
  • In other agar substances are added to exploit an organism's ability to produce a visible pigment (e.g.granada medium for Group B Streptococcus)which changes the bacterial colony's color, or to dissolve blood agar by hemolysis so that they can be more easily spotted. (wikipedia.org)
  • functions
  • The bacterial chromosome is a circular molecule of DNA that functions as a self-replicating genetic element (replicon). (nih.gov)
  • genomic
  • Here we describe the isolation and analysis of six genomic clones encoding terpene synthases of conifers, [(-)-pinene (C 10 ), (-)-limonene (C 10 ), ( E )-α-bisabolene (C 15 ), δ-selinene (C 15 ), and abietadiene synthase (C 20 ) from Abies grandis and taxadiene synthase (C 20 ) from Taxus brevifolia ], all of which are involved in natural products biosynthesis. (genetics.org)
  • System
  • This bacterial system might also be able to provide some utility for the analysis of eukaryotic Mg2+ transport proteins, but differences in biological systems of prokaryotes and eukaryotes will have to be considered in any experiment. (wikipedia.org)
  • study
  • A research study was completed by a Harvard Medical School professor at Brigham and Women's Hospital, Andrew B. Onderdonk, PhD. The study has revealed GLB44's strength and effectiveness against bacterial pathogens. (wikipedia.org)
  • high
  • In order to isolate organisms in materials with high microbial content, such as sewage, soil or stool, serial dilutions will increase the chance of separating a mixture. (wikipedia.org)
  • cell
  • It induces directed polymerization of actin by the ActA transmembrane protein, thus pushing the bacterial cell around. (wikipedia.org)
  • About half of the dry mass of a bacterial cell consists of carbon, and also about half of it can be attributed to proteins. (wikipedia.org)
  • As in other organisms, the bacterial cell wall provides structural integrity to the cell. (wikipedia.org)
  • The bacterial cell wall differs from that of all other organisms by the presence of peptidoglycan which is located immediately outside of the cytoplasmic membrane. (wikipedia.org)
  • Peptidoglycan is responsible for the rigidity of the bacterial cell wall and for the determination of cell shape. (wikipedia.org)
  • While all bacterial cell walls (with a few exceptions e.g. extracellular parasites such as Mycoplasma) contain peptidoglycan, not all cell walls have the same overall structures. (wikipedia.org)
  • There are two main types of bacterial cell walls, those of gram-positive bacteria and those of gram-negative bacteria, which are differentiated by their Gram staining characteristics. (wikipedia.org)
  • If the bacterial cell wall is entirely removed, it is called a protoplast while if it's partially removed, it is called a spheroplast. (wikipedia.org)
  • Blood
  • In other agar substances are added to exploit an organism's ability to produce a visible pigment (e.g.granada medium for Group B Streptococcus)which changes the bacterial colony's color, or to dissolve blood agar by hemolysis so that they can be more easily spotted. (wikipedia.org)
  • host
  • The gastrointestinal tract of humans is colonized by a dense microbial community that has co-evolved with its host to become a vital component of our biology. (biomedcentral.com)