• vitro
  • The researchers utilized atomic force microscopy to measure the binding force between a staphylococcal adhesion protein (SdrG from Staphylococcus epidermidis) and its cognate ligand (fibrinogen β) at the single-molecule level in vitro, and calculated the contributions of all of the atoms involved in the interaction in silico, with the help of an especially powerful supercomputer. (uni-muenchen.de)
  • Functional testing of F2 was performed by semiquantitative in situ adhesion assay on sections of human gastric mucosa and by quantitative in vitro adhesion assay with FITC-labled H. pylori strain J99 and human stomach AGS cells. (diva-portal.org)
  • The in vitro effect of treated surfaces on two oral bacteria strains (Streptococcus sanguinis and Lactobacillus salivarius) was studied with viable bacterial adhesion measurements and growth curve assays. (upc.edu)
  • No negative effects in fibroblast cell viability were detected and a significant reduction on bacterial adhesion in vitro was achieved in silver-treated samples compared with control titanium. (upc.edu)
  • A number of approaches have been used, in both in vitro and in vivo experimental models, to inhibit bacterial attachment to temporally expressed host receptors. (microbiologyresearch.org)
  • strains
  • All of the bacterial strains have negative zeta potential at experimental pH (5.5 and 5.8) and less become negative at higher ionic strength in both mono and divalent salt solutions. (wikipedia.org)
  • The objective of this study was to investigate the effect of nitrogen starvation on cell surface hydrophobicity and cell adhesion to soil particles by bacterial strains previously characterized as able to use benzene, toluene or xilenes as carbon and energy sources. (ufv.br)
  • Cultivation of the cells in nitrogen-deficient medium caused a significant reduction of the adhesion to soil particles by all the four strains. (ufv.br)
  • interactions
  • For numerous reasons, though, biochemical and structural biology knowledge of the molecular interactions involved in adhesion are limited. (springer.com)
  • This phenomenon has key role in host-pathogen interactions, endotoxic shock in patients, invasion and infection of animals/plants, inter-species bacterial competition, quorum sensing, exocytosis, etc. (see External links). (wikipedia.org)
  • protein
  • This figure shows how the staphylococcal adhesion protein (in green) interacts with its cognate peptide ligand (red). (uni-muenchen.de)
  • The study shows that, thanks to the geometry of the interaction, the adhesion protein forms a dense network of non-covalent hydrogen bonds with its ligand. (uni-muenchen.de)
  • In other words, the extremely high mechanical strength of the interaction is largely independent of both the amino-acid sequence of the adhesion protein and the biochemical properties of the target peptide. (uni-muenchen.de)
  • The dynamics of protein cluster localization suggest that intracellular motors and force transmission by dynamic focal adhesions can power bacterial motility. (sciencemag.org)
  • Mechanisms
  • Controlling and preventing the adverse impact of the bacterial deposition on the aquatic environment need a deeply understanding about the mechanisms of this process. (wikipedia.org)
  • pili
  • If the colonists are not immediately separated from the surface, they can anchor themselves more permanently using cell adhesion structures such as pili. (wikipedia.org)
  • membrane
  • While studying the critical tension required to detach a membrane bound to a surface through adhesion molecules, it was found that some adhesion molecules bound more tightly in response to tensions higher than the critical tension. (wikipedia.org)
  • In the prokaryotic gram-negative bacterial cells, membrane vesicle trafficking is mediated via bacterial outer membrane bounded nano-sized vesicles, called bacterial outer membrane vesicles (OMVs). (wikipedia.org)
  • A mechanism has already been proposed for generation of OMVs by gram negative microbes involving, expansion of pockets of periplasm (named, periplasmic organelles) due to accumulation of bacterial cell secretions and their pinching off as outer membrane bounded vesicles (OMVs) on the lines of a 'soap bubble' formation with a bubble tube, and further fusion or uptake of diffusing OMVs by host/target cells (Fig. 2). (wikipedia.org)
  • Molecules
  • IL-1, TNFα and C5a cause the endothelial cells of blood vessels near the site of infection to express cellular adhesion molecules, including selectins. (wikipedia.org)
  • infection
  • Methods of inhibiting bacterial adhesion to medical implants and reducing device-associated infection are effectuated by administering an effective amount of apo-transferrin to an individual with such an implant. (google.com)
  • mechanism
  • LMU researchers have characterized the physical mechanism that enables a widespread bacterial pathogen to adhere to the tissues of its human host. (uni-muenchen.de)
  • In order to dissect the adhesion mechanism, the Blue Waters supercomputer at the University of Illinois, with its 900,000 processors one of the most advanced in the world, carried out detailed molecular dynamics simulations. (uni-muenchen.de)
  • respiratory
  • Does upregulated host cell receptor expression provide a link between bacterial adhesion and chronic respiratory disease? (tcd.ie)
  • O'Toole R.F, Shukla S.D, Walters E.H, Does upregulated host cell receptor expression provide a link between bacterial adhesion and chronic respiratory disease? (tcd.ie)
  • cells
  • but inhibited bacterial binding to human gastric cells. (diva-portal.org)
  • The characterization of the physiological responses of the inoculated microorganisms to starvation, especially the evaluation of characteristics that affect the adhesion of the cells to soil particles, is fundamental to anticipate the success or failure of bioaugmentation. (ufv.br)
  • We found that A motility involved transient adhesion complexes that remained at fixed positions relative to the substratum as cells moved forward. (sciencemag.org)
  • By measuring the adhesion of single cells, important information for different topics in biology and materialscience can be obtained. (wikipedia.org)
  • With FluidFM it is possible to increase the rate in which these experiments can be performed, and even to assess the adhesion of spread cells. (wikipedia.org)
  • Those cancer cells can form cell-cell adhesions again and return to an epithelial state. (wikipedia.org)
  • The physical and chemical characteristics of bacterial cells can be affected by EPS composition, influencing factors such as cellular recognition, aggregation, and adhesion in their natural environments. (wikipedia.org)
  • The smaller size and single domain make these antibodies easier to transform into bacterial cells for bulk production, making them ideal for research purposes. (wikipedia.org)
  • deposition
  • It was concluded that bacterial deposition mainly occurred in a secondary energy minimum by using DLVO theory. (wikipedia.org)
  • This resulted to an observable bacterial deposition despite of the very high electrostatic repulsive energy from the DLVO prediction. (wikipedia.org)
  • investigate
  • The purpose of this book is thus to overcome this gap in communication between researchers in biology, chemistry and physics and to display the many ways and means to investigate bacterial adhesion. (springer.com)
  • tissues
  • This accumulation of microorganisms subject the teeth and gingival tissues to high concentrations of bacterial metabolites which results in dental disease. (wikipedia.org)
  • efficiency
  • It is inferred that the efficiency of bacterial transport through soils might be potentially increased by nitrogen starvation. (ufv.br)
  • affects
  • There is evidence that the adhesion and metal-binding ability of EPS affects mineral leaching rates in both environmental and industrial contexts. (wikipedia.org)
  • experiments
  • Flow flied at the stagnation point flow http://www.yale.edu/env/alexis_folder/alexis_research_2b.jpg Many bacterial stains have been used for the experiments. (wikipedia.org)
  • Microbial genetics experiments have greatly enhanced our knowledge of what bacterial factors are involved in adhesion. (springer.com)
  • A larger diameter is helpful for single cell adhesion experiments, whereas a smaller diameter provides good opportunities for nanolithography and handling of sub-micron objects. (wikipedia.org)
  • In addition to standard AFM experiments, FluidFM provides the possibility to perform countless other applications, such as single cell injection and adhesion as well as nanolithography and spotting. (wikipedia.org)
  • integrity
  • According to the researchers, "Robust cadherin adhesion is essential for maintaining the integrity of tissue such as the skin, blood vessels, cartilage and muscle that are exposed to continuous mechanical assault. (wikipedia.org)
  • process
  • Genetic transformation is the process by which a recipient bacterial cell takes up DNA from a neighboring cell and integrates this DNA into the recipient's genome by homologous recombination. (wikipedia.org)