Bacterial dna binding proteins. Medical search. Frequent questions

Examples include the HU protein in Escherichia coli, a dimer of closely related alpha and beta chains and in other bacteria can be a dimer of identical chains. (wikipedia.org)
HU-type proteins have been found in a variety of bacteria (including cyanobacteria) and archaea, and are also encoded in the chloroplast genome of some algae. (wikipedia.org)
Histone-like proteins were unknown to be present in bacteria until similarities between eukaryotic histones and the HU-protein were noted, particularly because of the abundancy, basicity, and small size of both of the proteins. (wikipedia.org)
Upon further investigation, it was discovered that the amino acid composition of HU resembles that of eukaryotic histones, thus prompting further research into the exact function of bacterial DNA binding proteins and discoveries of other related proteins in bacteria. (wikipedia.org)
Currently, many more functions of bacteria DNA binding proteins have been discovered, including the regulation of gene expression by histone-like nucleoid-structuring protein, H-NS. (wikipedia.org)
H-NS is about 15.6 kDa and assists in the regulation of bacterial transcription in bacteria by repressing and activating certain genes. (wikipedia.org)
In E. coli (and other Gram-negative bacteria) the cyclic AMP receptor protein (CRP/CAP) regulates more than 500 genes. (ku.dk)
B. cenocepacia make the protein as a weapon against other bacteria, so the protein on its own is toxic to cells. (acs.org)
At least four phylogenetically distinct groups of bacteria encode repeat proteins with the common ability to bind specific DNA sequences with a unique but conserved code. (uni-muenchen.de)
This unity of terminology belies disunity in the lifestyles of these different bacteria, and the biological roles fulfilled by these proteins. (uni-muenchen.de)
2, 6 Based on 16S ribosomal DNA comparison, these ancient bacteria show remarkable similarity to Bacillus marismortui , (named after the Dead Sea) and Virgobacillus pantothenticus . (creation.com)
Bacteria were used as sensitive indicators for DNA damage and mammalian liver extracts for metabolic conversion of carcinogens to their active metabolic forms. (europa.eu)
Bacteria display a series of mechanisms to control conjugative DNA transfer, as they do for any other physiological process ( Getino and de la Cruz, 2017 ). (frontiersin.org)
A LEUCINE and DNA-binding protein that is found primarily in BACTERIA and ARCHAEA. (bvsalud.org)
Researchers at the Max Planck Institute of Biophysics and Goethe University in Frankfurt have now gained new insights into exactly how bacteria import DNA. (mpg.de)
Many are actually snippets of DNA from viruses that are known to attack bacteria. (ieltsmaterial.com)
An important factor of bacterial virulence is the formation of biofilms which are aggregates of microorganisms incorporated into an extracellular matrix that protects bacteria from hostile environments. (usp.br)
When electron microscopy was used, there was no evidence that virus particles bound directly to bacteria. (lu.se)

We showed that strains of Pseudomonas aeruginosa with increased mutation rates (caused by deletion of genes involved in DNA mismatch repair) produced more biofilm biomass in laboratory experiments. (southampton.ac.uk)
The bacterial activator protein NorR binds to enhancer-like elements, upstream of the promoter site, and activates 54-dependent transcription of genes that encode nitric oxide detoxifying enzymes (NorVW), in response to NO stress. (strath.ac.uk)
The eukaryotic transactivation domain, which lends this group their name, allows them to activate specifically targeted host genes for the benefit of the bacterial invader. (uni-muenchen.de)
Genes can be regarded as blueprints for all of the molecular machines -normally proteins-that perform the tasks an organism needs for survival. (sciencedaily.com)
This is done through regulation of the activity of proteins that already exist in the cell, but also by the binding of special proteins-transcription factors-to specific sites on the DNA, turning certain genes on or off, which in turn regulates the cell's production of various proteins. (sciencedaily.com)
DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract. (wikidata.org)
Bacterial cells often use two-component signal transduction systems to regulate genes in response to environmental stimuli. (ntnu.edu.tw)
It was only at the tail end of the 1980s that researchers studying Escherichia coli noticed that there were some odd repetitive sequences at the end of one of the bacterial genes. (ieltsmaterial.com)
A bacterial cell generates special proteins from genes associated with the CRISPR repeats (these are called CRISPR associated - Cas - proteins). (ieltsmaterial.com)
In this sense, we aim to characterize the behaviour of different promoters of genes involved in biofilm regulation under different conditions and to search for possible new DNAbinding proteins associated with their promoter regions. (usp.br)
Nesse sentido, objetivamos caracterizar o comportamento de diferentes promotores de genes envolvidos na regulação de biofilme de P. aeruginosa sob diferentes condições e buscar possíveis novas proteínas de ligação ao DNA associadas às suas regiões promotoras. (usp.br)
Mitochondrial DNA contains 37 genes, all of which are essential for normal mitochondrial function. (medlineplus.gov)
The remaining genes provide instructions for making molecules called transfer RNA (tRNA) and ribosomal RNA (rRNA), which are chemical cousins of DNA. (medlineplus.gov)
The mitochondrial genes associated with cytochrome c oxidase deficiency provide instructions for making proteins that are part of a large enzyme group (complex) called cytochrome c oxidase (also known as complex IV). (medlineplus.gov)

In these high salt concentrations, the eukaryotic histone protein is eluted from a DNA solution in which single stranded DNA is bound covalently to cellulose. (wikipedia.org)
The role of single-stranded DNA binding (SSB) protein during DNA replication in Escherichia coli cells has been studied, specifically the interactions between SSB and the χ subunit of DNA polymerase III in environments of varying salt concentrations. (wikipedia.org)
Cloned p11.6 bound both double and single stranded DNA-cellulose columns. (illinois.edu)
Twinkle ( T7 gp4-like protein with intramitochondrial nucleoid localization, also known as C10orf2, PEO1, SCA8, ATXN8, IOSCA, PEOA3 or SANDO) is a homohexameric DNA helicases which unwinds short stretches of double-stranded DNA in the 5' to 3' direction and, along with mitochondrial single-stranded DNA binding protein and mtDNA polymerase gamma, is thought to play a key role in mtDNA replication. (nih.gov)
In Escherichia coli, a DNA mismatch repair (MMR) pathway corrects errors that occur during DNA replication by coordinating the excision and re-synthesis of a long tract of the newly-replicated DNA between an epigenetic signal (a hemi-methylated d(GATC) site or a single-stranded nick) and the replication error after the error is identified by protein MutS. (duke.edu)
Structural Mechanisms of Cooperative DNA Binding by Bacterial Single-Stranded DNA-Binding Proteins. (jhmi.edu)

In molecular biology, bacterial DNA binding proteins are a family of small, usually basic proteins of about 90 residues that bind DNA and are known as histone-like proteins. (wikipedia.org)
These dinoflagellate histone-like proteins replace histone in some dinoflagellates and package DNA into a liquid-crystalline state. (wikipedia.org)
Histone-like proteins are present in many Eubacteria, Cyanobacteria, and Archaebacteria. (wikipedia.org)
Here we describe an African swine fever virus (ASFV) protein encoded by the open reading frame 5-AR that shares structural and functional similarities with the family of bacterial histone-like proteins which include histone-like DNA binding proteins, integration host factor, and Bacillus phage SPO1 transcription factor, TF1. (illinois.edu)

In the case of the RipTALs this work should begin to unravel the role these proteins play in bacterial wilt disease, as a means to fight this devastating pathogen. (uni-muenchen.de)

Amoxicillin inhibits bacterial cell wall synthesis by binding to penicillin-binding proteins. (medscape.com)
Resistance is caused by a change in penicillin-binding proteins. (medscape.com)
Binds to one or more penicillin-binding proteins, which, in turn, inhibits synthesis of bacterial cell walls. (medscape.com)

Fig. 1: Photochemistry of light-oxygen-voltage receptors and sequences of proteins under study. (nature.com)
Mitochondrial DNA has remained inaccessible to those methods because the guide RNA molecules used to target specific DNA sequences to be edited can't get into mitochondria. (acs.org)
Protein recruitment is essentially a form of protein recognition, made possible by the presence of specific amino-acid sequences within the protein structure. (mechanobio.info)
This is achieved through the recognition of distinct signal sequences that target proteins to each of these regions. (mechanobio.info)
Those 'spacer' sequences look odd because they aren't bacterial in origin. (ieltsmaterial.com)
In 2005, three research groups independently reached the same conclusion: CRISPR and its associated genetic sequences were acting as a bacterial immune system. (ieltsmaterial.com)

Unique to the norVW promoter in Escherichia coli is the presence of three enhancer sites associated with a binding site for 54-RNA polymerase. (strath.ac.uk)

Consistent with a DNA binding function, immunoelectronmicroscopy localized p11.6 to the virion nucleoid. (illinois.edu)
We propose that RBM-bound RefZ complexes function as a developmentally regulated nucleoid occlusion system for fine-tuning the position of the septum relative to the chromosome during sporulation.IMPORTANCE The bacterial nucleoid forms a large, highly organized structure. (tamu.edu)
Thus, in addition to storing the genetic code, the nucleoid harbors positional information that can be leveraged by DNA-binding proteins to spatially constrain cellular activities. (tamu.edu)
During B. subtilis sporulation, the nucleoid undergoes reorganization, and the cell division protein FtsZ assembles polarly to direct septation over one chromosome. (tamu.edu)

In the other data set, mutants of a bacterial enzyme that controls resistance to penicillins and cephalosporins, TEM-1 beta-lactamase, were expressed in E. coli under the selective pressure of an antibiotic treatment. (frontiersin.org)
The penicillinase-resistant, or second-generation, penicillins are semisynthetic modifications of natural penicillins that are resistant to bacterial enzyme beta-lactamase, which accounts for typical penicillin resistance. (medscape.com)
The findings show how a crucial enzyme in our blood, known as ADAMTS13, works like a pair of molecular scissors to carefully cut back the clotting effects of a key protein, von Willebrand factor (VWF). (nottingham.ac.uk)
By resolving the crystal structure of the functional domains of ADAMTS13, the research reveals how after binding VWF, the enzyme must change its shape to open the active site and in turn specifically accommodate the cleavage site in VWF. (nottingham.ac.uk)

The use of bactericidal rather than bacteriostatic agents as first-line therapy is recommended because the eradication of microorganisms serves to curtail, although not avoid, the development of bacterial resistance. (cdc.gov)
So far, colistin has been best characterized with respect to its structure and biosynthesis, antibacterial mechanism and bacterial resistance, and toxicity and derivatives. (hindawi.com)
E. coli Rep helicase and RecA recombinase unwind G4 DNA and are important for resistance to G4-stabilizing ligands. (jhmi.edu)
But, of course, we also want to identify target structures that could inhibit DNA transfer and, for example, stem the spread of antibiotic resistance," D'Imprima says. (mpg.de)

For example, many membrane bound proteins carry signal peptides that are recognized by signal receptors that guide them to the target site. (mechanobio.info)

This can contribute to biofilm dispersal, and provides a mechanism for the release of extracellular DNA (eDNA) within biofilms. (southampton.ac.uk)
The mechanism of DNA binding is conserved. (uni-muenchen.de)
The free living B. marismortui have supposedly experienced 250 million years of mutations and a much larger difference to their isolated counterparts would be expected, 2, 6 although an efficient DNA repair mechanism during germination could partly explain the similarity. (creation.com)
Mechanism of activation and activity of bacterial RNase (VapBC, RelBE, HicAB) and kinase (HipBA) toxin-antitoxin systems. (au.dk)
Our analysis suggests that the bending action of HU on DNA is mediated by a mechanical clamping mechanism, in which metastable structural intermediates sampled during the hinge motions of the β-arms in the free state are presculpted to bind DNA. (ox.ac.uk)
The increased attachment may be one mechanism by which viruses precondition the respiratory mucosa for bacterial infection. (lu.se)

in these processes, bacterial DNA binding proteins have an architectural role, maintaining structural integrity as transcription, recombination, replication, or any other DNA-dependent process proceeds. (wikipedia.org)
Contributing to the interest of the present topic is the fact that modulation of gene activity involves the sensing of intra- and inter-cellular conditions, DNA binding and DNA dynamics, and interaction with the replication/transcription machinery of the cell. (ibecbarcelona.eu)
The latter might seem impossible, as an arbitrary transcription factor normally exists in just a handful of copies inside a bacterial cell, and one of them has to find a specific binding site on the DNA spiral, which contains some five million base pairs, in order to turn a gene on or off," says Erik Marklund, one of the lead authors of the new study. (sciencedaily.com)
Roughly 40 years ago it was observed that these transcription factors find their binding sites on DNA much more quickly that free diffusion in three dimensions would allow. (sciencedaily.com)
Theoretical and empirical studies have shown that it is likely that the transcription factors bind to a chromosome wherever they encounter one and then glide along the DNA in search of their binding sites. (sciencedaily.com)
Using large-scale computer simulations, researchers in Johan Elf's research team at Science for Life Laboratory at Uppsala University managed to study in detail how the transcription factor LacI moves along DNA in a spiral path. (sciencedaily.com)
From this comparison, the scientists derived the average time the transcription factor is bound to the DNA and how much of the DNA it has time to search through before it lets go. (sciencedaily.com)
Genome regulation encompasses all facets of gene expression, from the biochemical modifications of DNA, to the physical arrangement of chromosomes and the activity of the transcription machinery. (mechanobio.info)
Functional instability allows access to DNA in longer transcription Activator-Like effector (TALE) arrays. (jhmi.edu)
RefAuthors Lekstrom-Himes, J. A., Dorman, S. E., Kopar, P., Holland, RefAuthors S. M., Gallin, J. I. RefTitle Neutrophil-specific granule deficiency results from a RefTitle novel mutation with loss of function of the transcription RefTitle factor CCAAT/enhancer binding protein epsilon. (lu.se)
Published September 5, 2007 transcription factors and replicating extra DNA, rather than going directly into growth of the cell. (lu.se)

Trouw L., Nilsson S., Goncalvez I., Landberg G. and Blom A. M. (2005) C4b-binding protein binds to necrotic cells and DNA, which limits DNA release and inhibits complement activation. (lu.se)

It interferes with bacterial cell wall synthesis during active replication, causing bactericidal activity against susceptible organisms. (medscape.com)
First-generation semisynthetic cephalosporin that arrests bacterial cell wall synthesis, inhibiting bacterial growth. (medscape.com)
Control of eukaryotic messenger RNA synthesis by sequence-specific DNA-binding proteins. (wikidata.org)
The increase in adherence of S. pneumoniae could be inhibited by the DNA synthesis inhibitor cytosine arabinofuranoside, which is known to block the late phase of the adenovirus infection. (lu.se)

Scholars@Duke publication: A 'Semi-Protected Oligonucleotide Recombination' Assay for DNA Mismatch Repair in vivo Suggests Different Modes of Repair for Lagging Strand Mismatches. (duke.edu)
Standard ChIP workflows require a starting sample quantity of around 10 6 to 10 7 cells, below which the assay is hindered by high background binding, poor enrichment efficiencies and loss of enriched library complexity. (abcam.com)
While bacterial DNA is sometimes used as a blocking agent for standard ChIP, it is not advised for low-input ChIP as it carries through the assay and confounds data analysis . (abcam.com)
The Abcam high-sensitivity ChIP assay employs a unique chimeric protein to capture the antibody-bound protein:DNA complex and this offers significant advantage: the capture protein is smaller than Protein A or G and is coated at high density on the surface of microtiter plate wells, providing a much higher number of IgG immobilization sites in a smaller area, which in turn ensures efficiency and concentration of eluted DNA. (abcam.com)

In the nonobstetric population, chronic endometritis has been seen with infections (eg, chlamydia, tuberculosis, bacterial vaginosis) and the presence of an intrauterine device. (medscape.com)
Viruses are thought to facilitate bacterial infections of the respiratory tract, but the mechanisms are poorly understood. (lu.se)
Adenovirus (types 1, 2, 3, and 5) commonly causing respiratory tract infections increased the binding of. (lu.se)
Adenovirus (types 1, 2, 3, and 5) commonly causing respiratory tract infections increased the binding of adherent S. pneumoniae strains to the cells. (lu.se)
An important concept in the evaluation of data regarding transfusion-transmitted bacterial infections (TTBIs) is the definition of a case. (medscape.com)

In this study we show that exposure of isolated dimeric CRP-cAMP to UV modifies specific Met, Trp, Tyr, and Pro side-chains, induces inter-protein Tyr63-Tyr41 cross-links, and decreases DNA binding via oxidation of Met114/Pro110 residues in close proximity at the CRP dimer interface. (ku.dk)
UV exposure also modifies DNA-bound cAMP-CRP, with this resulting in DNA cleavage at specific G/C residues within the sequence bound to CRP, but not at other G/C sites. (ku.dk)
The modifications at the CRP dimer interface, and the site-specific DNA strand cleavage are proposed to occur via oxidation of two species Met residues (Met114 and Met189, respectively) to reactive persulfoxides that damage neighbouring amino acids and DNA bases. (ku.dk)
The RstA of Klebsiella pneumoniae consists of a N-terminal receiver domain (NRD, residues 1-119) and a C-terminal DNA-binding domain (DBD, residues 130-236). (ntnu.edu.tw)

Repeat arrays of these proteins have been demonstrated or predicted to form almost identical tertiary structures: a right-handed super helix that wraps around the DNA double strand with the base specifying residue of each repeat positioned in the major groove next to its cognate target base. (uni-muenchen.de)
The tertiary structures of these proteins are very similar although their amino acid compositions are significantly different. (thermofisher.com)
Recent improvements in the accuracy of structure-based methods for the prediction of nuclear magnetic resonance chemical shifts have inspired numerous approaches for determining the secondary and tertiary structures of proteins. (ox.ac.uk)

In DNA replication at the lagging strand site, DNA polymerase III removes nucleotides individually from the DNA binding protein. (wikipedia.org)
An unstable SSB/DNA system would result in rapid disintegration of the SSB, which stalls DNA replication. (wikipedia.org)
Research has shown that the ssDNA is stabilized by the interaction of SSB and the χ subunit of DNA polymerase III in E. coli, thus preparing for replication by maintaining the correct conformation that increases the binding affinity of enzymes to ssDNA. (wikipedia.org)
Furthermore, binding of SSB to DNA polymerase III at the replication fork prevents dissociation of SSB, consequently increasing the efficiency of DNA polymerase III to synthesize a new DNA strand. (wikipedia.org)

the protein is involved in stabilizing the lagging strand as well as interacting with DNA polymerase III. (wikipedia.org)
It has been found that H-NS and RNA polymerase both bind to the P1 promoter and form a complex. (wikipedia.org)
When H-NS is bound with RNA Polymerase to the promoter region, there are structural differences in the DNA that are accessible. (wikipedia.org)

Approximately 44% more IgG from rat serum bound to Protein G when Protein G Binding Buffer was used as compared with 20 mM Tris Buffer, pH 7.5. (thermofisher.com)
it occurs when mannose-binding lectin (MBL), a serum protein, binds to mannose, fucose, or N -acetylglucosamine groups on bacterial cell walls, yeast walls, or viruses. (msdmanuals.com)

Following elution, the protein readily binds DNA, indicating the protein's high affinity for DNA. (wikipedia.org)
Some applications, including 2D SDS-PAGE and solution phase proteolytic digestion for mass spectrometry, may require an additional detergent removal step following protein elution. (qiagen.com)

In E. coli, H-NS binds to a P1 promoter decreasing rRNA production during stationary and slow growth periods. (wikipedia.org)

The integration host factor (IHF), a dimer of closely related chains which is suggested to function in genetic recombination as well as in translational and transcriptional control is found in Enterobacteria and viral proteins including the African swine fever virus protein A104R (or LMW5-AR). (wikipedia.org)
Adherence was not affected by pretreatment of the cells with virus particles or viral proteins. (lu.se)

Research interests include determining protein structures and studying the structure/function of complexes formed with drugs and natural ligands. (nottingham.ac.uk)
We also have collaborative interests in determining structures of protein complexes formed with anti-cancer and anti-thrombosis inhibitors. (nottingham.ac.uk)
Crystal structures of the recombinant β-factor XIIa protease with bound Thr-Arg and Pro-Arg substrate mimetics. (nottingham.ac.uk)
The cytoskeleton is a highly dynamic network of filamentous proteins that enables the active transport of cellular cargo, transduces force, and when assembled into higher-order structures, forms the basis for motile cellular structures that promote cell movement. (mechanobio.info)

Because Protein G has greater affinity than Protein A for most mammalian IgGs, it may be used for the purification of mammalian IgGs that do not bind well to Protein A. Protein G binds with significantly greater capacity than Protein A to several IgG subclasses such as human IgG3, mouse IgG1 and rat IgG2a. (thermofisher.com)

This process of inducing virulence in an otherwise harmless bacterium through prophage infection and integration of the prophage genome into the bacterial host genome is called lysogenic conversion 8 . (nature.com)
However, currently characterized DNA-cleaving pAgos require elevated temperatures (≥65°C) for their activity, making them less suitable for applications that require moderate temperatures, such as genome editing. (nih.gov)
One type of DNA has remained beyond the reach of precision genome editing techniques like CRISPR. (acs.org)
Then, that chunk of viral DNA gets carried back to the bacterial cell's genome where it is inserted - becoming a spacer. (ieltsmaterial.com)

Here, we report the functional and structural characterization of the siDNA-guided DNA-targeting pAgo from the mesophilic bacterium Clostridium butyricum (CbAgo). (nih.gov)
SCOP: Structural Classification of Proteins and ASTRAL. (berkeley.edu)
The structural biology laboratory utilises a variety of techniques including Protein Crystallography, Cryo-electron Microscopy and associated techniques such as Isothermal Titration Calorimetry, which are then combined with functional studies or serve as a basis for structure-based drug design. (nottingham.ac.uk)
We use bioinformatics analysis of bacterial (meta) genomes, classical biochemistry as well as hybrid methods in structural biology (x-ray crystallography, electron microscopy, and small-angle x-ray scattering) and microbiology (physiology and growth) in our work. (au.dk)
Structural basis for DNA unwinding at forked dsDNA by two coordinating Pif1 helicases. (jhmi.edu)
In the crown we may have discovered a crucial switch for the recognition and binding of DNA," says Edoardo D'Imprima of the Department of Structural Biology at the Max Planck Institute in Frankfurt. (mpg.de)
Blom A. M. , Kask L. and B. Dahlbäck (2001) Structural requirements for the complement regulatory activities of C4b-binding protein. (lu.se)
Kask L., Hillarp A., Ramesh B., Dahlbäck B., and Blom A. M. (2002) Structural requirements for the intra-cellular subunit polymerization of the complement inhibitor C4b-binding protein. (lu.se)

We discovered that exogenous nitric oxide can act as a signal for the dispersal of bacterial biofilms. (southampton.ac.uk)

RipTALs are effector proteins delivered during bacterial wilt disease caused by R. solanacearum strains. (uni-muenchen.de)

In order for subcellular processes to be carried out within defined compartments or cellular regions, mechanisms must exist to ensure the required protein components are present at the sites and at an adequate concentration. (mechanobio.info)
These results illustrate that using side-chain chemical shift data in conjunction with MD simulations can provide quantitative information about the free energy landscapes of proteins and yield detailed insights into their functional mechanisms. (ox.ac.uk)
Mark L., Lee W. H., Villoutreix B. O., Proctor D., Blackbourn, D., Spiller B. O. and Blom A. M. (2004) KSHV complement control protein mimics human molecular mechanisms for inhibition of the complement system. (lu.se)

The study, to be published in a coming issue of Proceedings of the National Academy of Sciences (PNAS), compares the energy required to break off the interaction with DNA with the energy needed to glide along the DNA and how many times a protein binds back to the same DNA strand before it starts to look elsewhere. (sciencedaily.com)

Our data further support the role of ppGpp participating in the coordinated regulation of the expression of bacterial factors required during infection. (ibecbarcelona.eu)
Sjöberg A., Trouw L., McGrath F., Hack E. C. and Blom A. M. (2006) Regulation of complement activation by C-reactive protein: targeting of the inhibitory activity of C4b-binding protein. (lu.se)

The majority of proteins function as monodispersed ordered species dissolved in intra- or extra-cellular aqueous fluids. (frontiersin.org)
Easy-to-use AllPrep Bacterial/Fungal DNA/RNA/Protein Kits isolate total nucleic acids and cellular proteins from Gram (+/-) bacterial cultures and fungal cell cultures. (qiagen.com)

A DNA-Binding Protein Tunes Septum Placement during Bacillus subtilis Sporulation. (tamu.edu)

Timely assembly of polar Z rings and precise capture of the chromosome in the forespore both require the DNA-binding protein RefZ. (tamu.edu)
To mediate its role in chromosome capture, RefZ must bind to specific DNA motifs (RBMs) that localize near the poles at the time of septation. (tamu.edu)

The TetR family protein RefZ binds DNA motifs (RBMs) localized near the poles at the time of division and is required for both timely FtsZ assembly and precise capture of DNA in the future spore compartment. (tamu.edu)

The events in signal transduction that govern MMP expression and activity and expression of inhibitory proteins are crucial for understanding wound healing, tumorigenesis, and certain genetic diseases. (stanford.edu)

A new type of base editor works on mitochondrial DNA (blue helix). (acs.org)
A bacterial toxin (yellow) is split into two pieces and each are guided to the spot to be edited on mitochondrial DNA by programmable DNA-binding proteins (orange and pink). (acs.org)
Being able to edit mitochondrial DNA is very important from both a basic biology and a disease perspective," says Vamsi K. Mootha, an expert on mitochondrial biology at Harvard Medical School and the Howard Hughes Medical Institute (HHMI), who was involved in the new study. (acs.org)
This genetic material is known as mitochondrial DNA or mtDNA. (medlineplus.gov)
In humans, mitochondrial DNA spans about 16,500 DNA building blocks (base pairs), representing a small fraction of the total DNA in cells. (medlineplus.gov)
The following chromosomal conditions are associated with changes in the structure or number of copies of mitochondrial dna. (medlineplus.gov)
Changes in mitochondrial DNA are among the best-studied genetic factors associated with age-related hearing loss. (medlineplus.gov)
As people age, mitochondrial DNA accumulates damaging mutations, including deletions and other changes. (medlineplus.gov)
Mitochondrial DNA is especially vulnerable because it has a limited ability to repair itself. (medlineplus.gov)
As a result, reactive oxygen species easily damage mitochondrial DNA, causing cells to malfunction and ultimately to die. (medlineplus.gov)
Cells that have high energy demands, such as those in the inner ear that are critical for hearing, are particularly sensitive to the effects of mitochondrial DNA damage. (medlineplus.gov)
Some cases of cyclic vomiting syndrome, particularly those that begin in childhood, may be related to changes in mitochondrial DNA. (medlineplus.gov)
Some of the genetic changes alter single DNA building blocks (nucleotides), whereas others rearrange larger segments of mitochondrial DNA. (medlineplus.gov)

The virus-infected cells were harvested at various times after infection and analyzed for the ability to bind strains of Haemophilus influenzae and Streptococcus pneumoniae. (lu.se)

For example, in epithelial cells, which are polarized, protein composition at the apical membrane is very different from that at the basolateral membrane. (mechanobio.info)
Cytosolic proteins that are associated with the plasma membranes are often localized based on their interaction with membrane lipids such as the phosphoinositides. (mechanobio.info)
The crown outside the membrane (grey bar) is an unknown protein responsible for DNA uptake through the secretin. (mpg.de)
Factor I, with cofactors including membrane cofactor protein (CD46), inactivates C3b and C4b. (msdmanuals.com)

It has also been found that H-NS can affect translation as well by binding to mRNA and causing its degradation. (wikipedia.org)
Other blocking agents such as inert proteins or mRNA can reduce background binding in low-input ChIP, without contaminating the data 2 . (abcam.com)

By contrast, in the case of TEM-1 beta-lactamase mutations, aggregation is linked to a decreased cell fitness due to inactivation of protein function. (frontiersin.org)
Some rare metabolic diseases are caused by mutations in this type of DNA. (acs.org)
Single molecule analysis of effects of non-canonical guide RNAs and specificity-enhancing mutations on Cas9-induced DNA unwinding. (jhmi.edu)
However, mutations in the gun-like structure cause the crown to disintegrate, so that the bacterial cell is no longer able to absorb DNA. (mpg.de)
The mtDNA mutations that cause this condition alter the proteins that make up cytochrome c oxidase. (medlineplus.gov)

An increase in bacterial mutation rate can promote biofilm formation. (southampton.ac.uk)
The key studies are considered to be bacterial mutation assays (McCann et al. (europa.eu)
Vinyl acetate was negative in bacterial gene mutation assays using Salmonella typhimurium TA98, TA100, TA1535 and TA1537, with and without rat liver S-9 activation. (europa.eu)
Vinyl acetate was shown to be negative in bacterial gene mutation assays using Salmonella typhimurium TA98, TA100, TA1535 and TA1537, with and without rat liver S-9 activation. (europa.eu)

In addition, in silico analysis of FI proteins suggests that they represent recent acquisitions of conjugative plasmids, i.e., are not shared by members of the same plasmid species. (frontiersin.org)

This group also contains viral GP4-like and related bacterial helicases. (nih.gov)

Determinants of target prioritization and regulatory hierarchy for the bacterial small RNA SgrS. (jhmi.edu)
Blom, A. M. , Rytkönen, A., Vasquez, P., Dahlbäck, B. and Johnsson, A-B. (2001) A novel interaction between type IV pili of Neisseria gonorrhoeae and complement regulatory protein - C4b-binding protein. (lu.se)

Small intestinal permeability was assessed using the lactulose/rhamnose dual sugar absorption test and small intestinal epithelial injury using Intestinal Fatty-Acid Binding Protein (I-FABP). (bvsalud.org)
The present study analyzed the effect of adenovirus on bacterial adherence to human respiratory tract epithelial cells. (lu.se)

Monospecific antiserum prepared to the 5-AR bacterial expression product specifically immunoprecipitated a protein of approximately 11.6kDa from ASFV infected swine macrophages at late times post-infection. (illinois.edu)
At the molecular level, it was found that replacement of glucose by starch has enhanced the relative expression level of ccpA encoding catabolite control protein A. Therefore, the repression of starch utilization by glucose could be probably relieved. (hindawi.com)
The expression of abrB itself is negatively controlled by Spo0A, another DNA-binding protein, encoded by spo0A [ 15 ]. (hindawi.com)
Researchers can now provide a picture of how proteins regulate genetic expression at the atomic level. (sciencedaily.com)
Alternatives have suggested the use of epitope tagged proteins 6 , but these run the risk of over-expression and the introduction of artefacts. (abcam.com)
DNA, is preferred when demand for expression is rare, whereas an sources present at levels that are perturbed at some point in time activating mode is preferred in the opposite situation [8]. (lu.se)

Its active agent is an antibiotic protein called SASP, which binds to bacterial DNA, inactivating it. (growthbusiness.co.uk)

It has been determined that colistin biosynthesis is negatively regulated by AbrB, a DNA-binding protein, by directly binding to the upstream region of pmxA [ 11 ]. (hindawi.com)
When conducting DNA-affinity chromatography pulldown for the upstream regions of retS and rsmA in LB media, we found pqsC and pqsH binding to the upstream region of retS and mvaT binding to the upstream region of rsmA. (usp.br)

Working together with others I was able to show that repeats from each group of TALE-likes mediate sequence specific DNA binding, revealing a conserved code in each case. (uni-muenchen.de)

Haemophilus influenzae b vaccine in the binding protein (PBP) is also of concern cultured on chocolate agar + polyvita- industrialized world and the decreased as it relates to 20% of non-encapsulated min supplement + bacitracin (Bio-rad, incidence of invasive diseases [1], Hae- strains isolated in Europe [9]. (who.int)

Centre for Bacterial Cell Biology, Institute for Cell and Molecular Biosciences, Newcastle University. (ncl.ac.uk)

The accumulation of a protein at a given site is known as protein localization. (mechanobio.info)
Webb, J. H., Villoutreix, B. O., Dahlbäck, B. and Blom A. M. (2001) Localization of a hydrophobic binding site for protein S on the b-chain of C4BP. (lu.se)

Each repeat binds a single DNA base, and specificity is determined by the amino acid residue at position 13 of each repeat. (uni-muenchen.de)

Anatomy11

Organisms27

Diseases4

Chemicals and Drugs111

Analytical, Diagnostic and Therapeutic Techniques and Equipment29

Phenomena and Processes76

Information Science4

Health Care3

Bacteria18

Genes14

Single-stranded6

Histone-like proteins4

Pathogen1

Penicillin-binding3

Sequences6

Escherichia1

Nucleoid4

Enzyme4

Resistance4

Peptides1

Mechanism6

Transcription11

Inhibits1

Synthesis4

Assay4

Infections5

Residues4

Tertiary structures3

Replication4

Polymerase3

Serum2

Elution2

Coli1

Viral proteins2

Structures4

Mammalian1

Genome4

Structural8

Nitric oxide1

Effector1

Mechanisms3

Strand1

Regulation2

Cellular2

Bacillus1

Chromosome2

Motifs1

Inhibitory1

Mitochondrial DNA13

Ability to bind1

Membrane4

MRNA2

Mutations5

Mutation4

Suggests1

Helicases1

Regulatory2

Epithelial2

Expression6

Antibiotic1

Upstream region2

Sequence specific1

Diseases1

Molecular Biosciences1

Localization2

Amino acid1