• Gene
  • Using advanced instruments, like the MicroSeqTM Fungal LSU rRNA Gene sequencing, GC-FAME, and Fungal BIOLOG to help identify mold and fungus. (environmental-expert.com)
  • The example is also noteworthy in that the sequences giving rise to the anomalous behavior lie entirely within the coding sequences for the major capsid protein (VP1) gene. (springer.com)
  • Second, isolated sequences can be derived directly from clone without knowing tissues expressing the gene which needs to be identified. (wikipedia.org)
  • The internal transcribed spacer 2 (ITS2) of the 45S rRNA gene was sequenced for micro B, large B, and A chromosomes of B. dichromosomatica cytodeme A2, and conserved differences were identified between sequences originating from A and both types of B chromosomes. (jove.com)
  • Pol III is unusual (compared to Pol II) by requiring no control sequences upstream of the gene, instead normally relying on internal control sequences - sequences within the transcribed section of the gene (although upstream sequences are occasionally seen, e.g. (wikipedia.org)
  • Typical stages in a tRNA (also termed class II) gene initiation: TFIIIC (Transcription Factor for polymerase III C) binds to two intragenic (lying within the transcribed DNA sequence) control sequences, the A and B Blocks (also termed box A and box B). TFIIIC acts as an assembly factor that positions TFIIIB to bind to DNA at a site centered approximately 26 base pairs upstream of the start site of transcription. (wikipedia.org)
  • Typical stages in a U6 snRNA (also termed class III) gene initiation (documented in vertebrates only): SNAPc (SNRNA Activating Protein complex) (also termed PBP and PTF) binds to the PSE (Proximal Sequence Element) centered approximately 55 base pairs upstream of the start site of transcription. (wikipedia.org)
  • citation needed] The types of RNAs transcribed from RNA polymerase III include: Transfer RNAs 5S ribosomal RNA U6 spliceosomal RNA RNase P and RNase MRP RNA 7SL RNA (the RNA component of the signal recognition particle) Vault RNAs Y RNA SINEs (short interspersed repetitive elements) 7SK RNA Several microRNAs Several small nucleolar RNAs Several gene regulatory antisense RNAs DNA polymerase III holoenzyme Vannini, A. (wikipedia.org)
  • Outbreaks
  • We conclude that high-throughput sequencing approaches can make a significant contribution to the investigation of outbreaks of bacterial infection and that the integration of WGS with epidemiological investigation, diagnostic assays and antimicrobial susceptibility testing will precipitate radical changes in clinical microbiology and infectious disease epidemiology in the near future. (biomedcentral.com)
  • silico
  • We have annotated 95% of the fingerprint map clones in contigs with coordinates on the version 3.1 rat genome sequence assembly, using BAC-end sequences and in silico mapping methods. (wustl.edu)
  • nucleotides
  • Minisatellites are a type of DNA tandem repeat sequence, meaning that the sequences repeat one after another without other sequences or nucleotides in between them. (wikipedia.org)
  • The sequences of minisatellites are larger than those of microsatellites, in which the repeat sequence is generally two to five nucleotides. (wikipedia.org)
  • VNTRs, therefore, because they have repeat sequences of ten to one hundred nucleotides in which every repeat is exactly the same, are considered minisatellites. (wikipedia.org)
  • These ICDSs generally result from the insertion or deletion of nucleotides, affecting the frame read and splitting the original coding sequence into two or more smaller open reading frames. (beds.ac.uk)
  • protein
  • The spa typing technique uses the sequence of a polymorphic VNTR in the 3' coding region of the S. aureus -specific staphylococcal protein A ( spa ). (applied-maths.com)
  • A phylogenetic tree is usually derived from DNA or protein sequences from populations. (wikipedia.org)
  • GenBank
  • However, with the modest exception of Alligator mississippiensis for which there is roughly 2.5 Mb of BAC end sequence [ 9 ] and 26 partially assembled BACs (GenBank, 10/3/2008), little sequence data is available for crocodilian genomes, in part due to a relative lack of high-quality molecular tools for this important clade. (biomedcentral.com)
  • Genome Survey Sequences are typically generated and submitted to NCBI by labs performing genome sequencing and are used, amongst other things, as a framework for the mapping and sequencing of genome size pieces included in the standard GenBank divisions. (wikipedia.org)
  • organisms
  • Because of certain technical advantages, including a highly compact genome of about 130 Mb with little interspersed repetitive DNA, Arabidopsis is one of the most widely used model organisms for studying the biology of higher plants. (nsf.gov)
  • similarity
  • Nucleotide and amino acid sequence alignment of the C. porosus C-mos coding sequence with avian and reptilian C-mos orthologs reveals greater sequence similarity between C. porosus and birds (specifically chicken and zebra finch) than between C. porosus and squamates (green anole). (biomedcentral.com)
  • typically
  • A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from 1-6 or more base pairs) are repeated, typically 5-50 times. (wikipedia.org)
  • segments
  • Controversial references to 'junk' or 'selfish' DNA were put forward early on, implying that repetitive DNA segments are remainders from past evolution or autonomous self-replicating sequences hacking the cell machinery to proliferate. (wikipedia.org)
  • unlike
  • GSSs can provide an initial global view of a genome, which includes both coding and non-coding DNA and contain repetitive section of the genome unlike ESTs. (wikipedia.org)
  • TFIIB remains bound to DNA following initiation of transcription by Pol III (unlike bacterial σ factors and most of the basal transcription factors for Pol II transcription). (wikipedia.org)
  • importantly
  • More importantly, Arabidopsis genome sequence information will be applicable to major crops, such as corn and soybeans, because of the close evolutionary similarities among the genomes of all flowering plants. (nsf.gov)
  • rapidly
  • If PCR is performed using Oxford Nanopore's modified primers, sequencing adapters can be attached rapidly following amplification, by chemical ligation. (nanoporetech.com)
  • base pairs
  • VNTRs are a type of minisatellite in which the size of the repeat sequence is generally ten to one hundred base pairs. (wikipedia.org)
  • This assembly is greatly stimulated by the Pol II transcription factors Oct1 and STAF that bind to an enhancer-like DSE (Distal Sequence Element) at least 200 base pairs upstream of the start site of transcription. (wikipedia.org)
  • analysis
  • Since fragment of DNA can be inserted into sequences, if an exon is inserted into intron, the transcript will be longer than usual and this transcript can be trapped by analysis. (wikipedia.org)
  • similar
  • The 3D folding of metazoan genomes correlates with the association of similar repetitive elementsA Cournac, R Koszul, J Mozziconacci - Nucleic Acids Research, 2016 Ussery, David W. (wikipedia.org)
  • Structures
  • Bacterial community fingerprinting refers to a number of cultivation-independent methods that are used to measure biodiversity in environmental samples or to profile microbial community structures, e.g. in response to experimental conditions. (applied-maths.com)
  • library
  • To investigate the utility of the library in studying sequence distribution, probes derived from CR1a and CR1b, two crocodilian CR1-like retrotransposon subfamilies, were hybridized to C. porosus macroarrays. (biomedcentral.com)
  • Following elution, we prepared a sequencing library in 5 minutes using our rapid 1D gDNA kit, and we loaded the library onto a flowcell. (nanoporetech.com)
  • Finally, the sequencing library is loaded onto a MinIONTM flowcell and the run started. (nanoporetech.com)
  • For the estimation of repetitive sequences, GSS plays an important role in the early assessment of a sequencing project since these data can affect the assessment of sequences coverage, library quality and the construction process. (wikipedia.org)