• coli
  • The results showed that although the P(3HB)s created differed slightly in properties and although E. coli had a higher P(3HB) content, C. glutamicum had almost four times higher cell density, making it a more efficient producer of the polyester. (kenyon.edu)
  • Traditionally, Escherichia coli is the choice bacterium to express eukaryotic and recombinant genes. (wikipedia.org)
  • Microbial production of Succinic acid can be performed with wild bacteria like Actinobacillus succinogenes, Mannheimia succiniciproducens and Anaerobiospirillum succiniciproducens or genetically modified Escherichia coli, Corynebacterium glutamicum and Saccharomyces cerevisia. (wikipedia.org)
  • For example, common hosts are bacteria (such as E.coli, B. subtilis), yeast (such as S.cerevisiae) or eukaryotic cell lines. (wikipedia.org)
  • For example, a DNA sequence for a protein of interest could be cloned or subcloned into a high copy-number plasmid containing the lac promoter, which is then transformed into the bacterium E. coli. (wikipedia.org)
  • In comparing C. glutamicum and E. coli, monomer and dimer, respectively, both enzymes were found to "efficiently catalyze identical reactions. (wikipedia.org)
  • However, C. glutamicum was recorded as having ten times as much activity than E. coli and seven times more affinitive/specific for NADP. (wikipedia.org)
  • Organism
  • Comprehensive screening of secretion signal sequences has been conducted in C. glutamicum R by using bioinformatic analysis and a high-throughput secretion assay, which identified a total of 108 candidate signal sequences that could secrete heterologous α-amylase in the organism. (asmscience.org)
  • Inactivation of both the PCx gene and the PEPCx gene in C. glutamicum led additionally to the inability to grow on glucose, indicating that no further anaplerotic enzymes for growth on carbohydrates exist in this organism. (uni-bielefeld.de)
  • pathogenic
  • DIR21 (accession number MCC 0081) that exhibits quenching of quorum sensing mechanism of a pathogenic bacteria Vibrio harveyi in an aquaculture. (sumobrain.com)
  • To combine the high yield/productivity and scalable protein features of bacteria and yeast, and advanced epigenetic features of plants, insects and mammalians systems, other protein production systems are developed using unicellular eukaryotes (i.e. non-pathogenic 'Leishmania' cells). (wikipedia.org)
  • microorganisms
  • In 1676, Antonie van Leeuwenhoek , who lived most of his life in Delft, Holland, observed bacteria and other microorganisms using a single-lens microscope of his own design. (wikipedia.org)
  • Microorganisms like yeast and bacteria are used to massively produce the many things. (wikipedia.org)
  • encodes
  • The chromosome of this bacterium encodes both fleQ (see below) and flhDC , but only the later operon is required for flagella synthesis ( Leon and Espin, 2008 ). (frontiersin.org)
  • antibiotics
  • In certain aspects, altering bacterial fitness slows or prevents development of adaptive resistance to antibiotics in bacteria. (sumobrain.com)
  • Non-Streptomyces actinomycetes, filamentous fungi, and non-filamentous bacteria, have also yielded important antibiotics. (wikipedia.org)
  • organisms
  • Louis Pasteur's discovery that food fermentations are caused by microscopic organisms - bacteria, simple fungi and yeasts. (eufic.org)
  • rRNA
  • Microbiologists often rely on molecular biology tools such as DNA sequence based identification, example 16s rRNA gene sequence used for bacteria identification. (wikipedia.org)
  • virulence
  • This bacterium is apparently equipped with a broad and varying set of virulence factors, including a novel type of a ribosome-binding protein. (uni-bielefeld.de)
  • analyses
  • PhoA fusion analyses of LysE of C. glutamicum provided evidence for a 5 transmembrane α-helical spanner (TMS) typology with the N-terminus inside and the C-terminus outside. (wikipedia.org)
  • Yeast
  • Commonly used protein production systems include those derived from bacteria, yeast,baculovirus/insect, mammalian cells, and more recently filamentous fungi such as Myceliophthora thermophila. (wikipedia.org)
  • Drinking alcohol also known as ethanol is produced by yeast and bacteria. (wikipedia.org)
  • enzyme
  • The invention provides a non-naturally occurring bacterium having decreased or eliminated activity of an enzyme that catalyzes the reaction defined by EC 1.2.7.5, such as aldehyde:ferredoxin oxidoreductase (AOR). (sumobrain.com)
  • production
  • Vinegar production was one of his major interests, a process which had historically had mixed success due to contamination by inappropriate bacteria. (eufic.org)
  • Methanol often inhibits growth of bacteria, thus, methanol tolerance may limit fermentative production processes. (uni-bielefeld.de)
  • plasmid
  • Plasmids were inserted into c. glutamicum , including an expression plasmid, that under certain conditions would create the P(3HB). (kenyon.edu)
  • lactose
  • Addition of IPTG (a lactose analog) activates the lac promoter and causes the bacteria to express the protein of interest. (wikipedia.org)
  • carbon
  • Inactivation of the chromosomal pyc gene in C. glutamicum WT led to the absence of PCx activity and to negligible growth on lactate, indicating that PCx is essential for growth on this carbon source. (uni-bielefeld.de)
  • found
  • Cyanophycin was first detected in 1887 by the Italian botanist Antonino Borzì and can be found in most cyanobacteria and a few heterotrophic bacteria such as Acinetobacter sp. (wikipedia.org)
  • Porins are water-filled pores and channels found in the membranes of bacteria and eukaryotes. (wikipedia.org)