Available biological assay. Medical search. Frequent questions

Methodologically, the study showed that silicone can be used as reference partitioning phase to transfer in vivo exposure in humans (silicone implants) to in vitro assays (partition-controlled dosing). (nih.gov)
The presentation will be focused on exploring mathematical models which are suitable to describe cargo-delivery using nanomaterials (NMs) while assessing information from in vitro assays. (worldnanotechnologyconference.com)
Presently, less than about 0.5% of the accumulated world's reports involving in vitro assays include a mathematical model, but this fraction is growing up steeply worldwide in recent years, signaling the recent trend in handling the biological data in a more comprehensive way. (worldnanotechnologyconference.com)
The starting mathematical model for in vitro assays is the Hill equation, herein described as a Hill-inspired approach, characterized by its Hill coefficient. (worldnanotechnologyconference.com)

Biological assays to test the cytotoxicity of the compound 1b combined with electroporation were performed to determine its potential for future medical applications in cancer treatment. (hindawi.com)
The negative results for cytotoxic and antimicrobial assays indicated possible low cytotoxicity to the extract. (researchgate.net)
A parallel cytotoxicity assay was also run without the 6-TG wild type cell. (cdc.gov)

In one run of the assay M molecules are tested, generating 2 M N data points. (physicsforums.com)
The assay is often repeated multiple times on certain molecules. (physicsforums.com)
Because aldehydes have the tendency to react with biological molecules to form various products, including Schiff base protein adducts, free aldehydes released into the headspace of biological samples from the Schiff base protein adducts at low pH (~3) are analyzed. (cdc.gov)
Biomolecular simulation is increasingly central to understanding and designing biological molecules and their interactions. (manchester.ac.uk)

Quantitative activation suppression assay to evaluate human bone marrow-derived mesenchymal stromal cell potency. (ctsicn.org)

What is a bioassay or biological assay? (pharmacologycorner.com)
The biological indicators in bioassay are the reactions of living organisms or tissues. (pharmacologycorner.com)
The term dilution assay is generally used to designate a special type of bioassay in which one or more preparations (e.g. a drug) are administered to experimental units at different dose levels inducing a measurable biological response. (wikipedia.org)
Combining bioassay screening with the cheminformatics strategy of LC-MS/MS molecular networking as a discovery tool expedited the targeted isolation of a natural product possessing both a novel chemical structure and a desired biological activity. (henryford.com)

To demonstrate its versatility, we implemented and validated the assay in vitro and in vivo for the bacterial Sec system and the mitochondrial protein import apparatus. (uni-luebeck.de)

The ATP Bioluminescence Kit provides a simple and quick assay to quantify ATP, and also to measure cell viability based on ATP content. (sigmaaldrich.com)

Assay biological specimens for biomarkers of disease. (nih.gov)

This training on assay validation will teach you how to validate an assay for clinical diagnostics and transition the assay into the clinical laboratory for diagnostic use. (complianceonline.com)
During the recent Zika outbreak, CDC developed and deployed two Zika diagnostic assays to LRN-B reference laboratories across the U.S. From March 2016 through April 2017, LRN-B reference laboratories tested and reported over 90,000 distinct results to CDC-a staggering volume. (cdc.gov)

Detection of intracellular granules/foci or other local intracellular particles is a valuable tool for a large variety of biological assays. (idea-bio.com)
We introduce the modification of bacteriophage particles with aptamers for use as bioanalytical reporters, and demonstrate the use of these particles in ultrasensitive lateral flow assays. (nih.gov)

ENVI Assay System test kits are featured with practical tools and straightforward test method giving fast response, specificity, and sensitivity ideal for provisional biological threat assessment from environmental samples. (j3seven.com)

Biological Assay" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (ctsicn.org)

Recently, identification of metabolites involved in the biotransformation of phytochemicals by intestinal microflora has been suggested as a potentially effective means to determine which compounds are active in living systems and to gain a better understanding of how herbs affect biological processes [ 11 , 12 ]. (hindawi.com)
Synthace's cloud-based platform Antha® enables automation of design of experiments (DOE) to optimize complex biological processes and assays. (jmp.com)

By the time you are finished with this seminar, you will be able to learn what needs to be done to an assay to make sure it is ready for the clinic and how to validate such changes. (complianceonline.com)
In addition, you will learn how to select a clinically relevant population for a given assay, validate the assay within such a population and how to select Gold Standards for comparison. (complianceonline.com)
This webinar will help you learn how to validate assays for clinical diagnostics. (complianceonline.com)
How to make sure an assay can regularly be performed by a medical technologist, and how to validate those changes? (complianceonline.com)
How to validate an assay for clinical use? (complianceonline.com)
Until now the standard procedure to validate antibodies for IP couples the IP assay to immunoblotting (IP/IB), a procedure that is challenging to scale to high-throughput analysis [ 10 ]. (biomedcentral.com)
To further validate and improve such biological and physical assays, in 2019, EURADOS Working Group 10 and RENEB performed a field exercise in Lund, Sweden, to simulate various real-life exposure scenarios. (lu.se)

Major gaps in our understanding of this process arise due the poor sensitivity, low time resolution and irreproducibility of translocation assays. (uni-luebeck.de)

With the advent of laboratory automation and more rigorous analytical techniques, scientists now have the opportunity to ask more complex questions to understand and develop more complex biological systems. (jmp.com)
The approval was based on "comprehensive review of scientific evidence," including "comparisons of the products on an analytical level using an extensive battery of chemical and biological tests and biological assays that confirmed similarity in the structural and functional features of Wezlana and Stelara (including those known to impact safety and efficacy), and comparative human pharmacokinetic data, clinical immunogenicity data, and other clinical safety and effectiveness data," the FDA said. (medscape.com)

ENVI Assay System allows for early detection and BWA identification, which combined with timely warning, protection and correct medical countermeasures are crucial steps for the whole process of bio-threat management. (j3seven.com)
These modified viral nanoparticles were used in immunochromatographic sandwich assays for the direct detection of IgE and of the penicillin-binding protein from Staphylococcus aureus (PBP2a). (nih.gov)
The limit of detection of this LFA was 0.13 ng/mL IgE, ∼100 times lower than those of previously reported IgE assays. (nih.gov)

For a mathematical definition of a dilution assay an observation space U {\displaystyle U} is defined and a function f : U → R {\displaystyle f:U\rightarrow \mathbb {R} } so that the responses u ∈ U {\displaystyle u\in U} are mapped to the set of real numbers. (wikipedia.org)

Statistical method in biological assay. (cdc.gov)
Statistical analysis of experimental designs applied to biological assays. (lu.se)

Unless an assay can give clinically actionable results in a clinical laboratory, whatever utility the assay may have will be useless to clinical practioners, who have different demands than research laboratories. (complianceonline.com)
Finally, you will be able to develop clinical quality monitoring standards to make sure the assay remains relevant in a clinical context. (complianceonline.com)
What are the key differences between a research assay and a clinical assay? (complianceonline.com)
How do you find clinical relevant samples to test your assay against? (complianceonline.com)
How to develop a clinical quality plan to make sure the assay remains valid? (complianceonline.com)
However, before employing a bioanalytical assay for routine purposes, assay qualification and validation become vital to demonstrate its suitability for clinical applications. (uscalifornia.com)
As a drug candidate reaches the clinical stages of drug development , bioanalytical assays will require full assay validation. (uscalifornia.com)

Hence robust bioanalytical assays will always remain crucial to maximize the generation of reliable and reproducible study data. (uscalifornia.com)
Synthace offers a new approach, by applying the principles of DOE, to reduce experimentation time and make biological research more reproducible. (jmp.com)

The immunoprecipitation (IP) assay is a valuable molecular biology tool applied across a breadth of fields. (biomedcentral.com)

Five concentrations of each test chemical were used in the metabolic cooperation assay. (cdc.gov)
Rapid methods of measuring the effects of an agent in a biological or chemical assay. (bvsalud.org)

The biological test system was a consortium of microorganisms common to the activated sludge treatment process. (europa.eu)
The same assay could also be used to test other biological activity. (sciencedaily.com)

In addition, these assays have to be able to handle clinically relevant samples, which often differ from the samples used in research studies. (complianceonline.com)
In the past decade, super-resolution fluorescence microscopy has revolutionized biological research by enabling the study of biological functions down to the molecular scale. (uni-goettingen.de)
The use of half-lives and associated confidence intervals in biological research. (cdc.gov)
The immunoprecipitation assay (IP) is an important tool used in academic and industrial research labs for applications such as targeted protein purification, protein concentration, analysis of protein-protein interactions, identification/analysis of protein complexes and analysis of protein/DNA interactions using chromatin IP (ChIP). (biomedcentral.com)
In Beersheva (Israel) research was carried out using radio-immuno assay of sexual hormones in the blood of the female. (fao.org)
Clean, document, disseminate, archive (including storage of biological specimens for future study), and promote the Wave VI data to the scientific community for aging research. (nih.gov)
PLOS Biology provides an Open Access platform to showcase your best research and commentary across all areas of biological science. (plos.org)
Today's approval exemplifies the FDA's longstanding commitment to support a competitive marketplace for biological products," Sarah Yim, MD, director of the Office of Therapeutic Biologics and Biosimilars in the FDA's Center for Drug Evaluation and Research, said in a statement . (medscape.com)

In the V79 assay, the amount of cell killing that occurred when toxic 6-thioguanine (154427) (6-TG) monophosphate from wild type cells was transferred to 6-TG resistant cells, was an indication of cell to cell communication inhibition. (cdc.gov)
The culture medium for the assay contained 3 percent fetal bovine serum, 100 6-TG resistant cells and 4x10(5) 6-TG wild type cells. (cdc.gov)

The Laboratory Response Network for Biological Threats (LRN-B) was created to strengthen the nation's ability to detect biological threat agents, like smallpox and anthrax. (cdc.gov)
45 distinct tests for biological threats, emerging infectious diseases, and other high-consequence pathogens-like Ebola, plague, and smallpox. (cdc.gov)

Newer bioanalytical assays are developed continuously to support drug candidates at various stages of development. (uscalifornia.com)

Bioanalytical Assay Exposed: The Key To Unlocking Insights Into Biological Samples! (uscalifornia.com)
Hence, whether in vitro assay development or fully validated bioanalytical methods, unlocking the potential of bioanalytical assays is crucial in gaining insights into biological samples. (uscalifornia.com)

Simulations can now be used as computational assays of biological activity, for example, in predictions of drug resistance. (manchester.ac.uk)
With this assay, they tested the vapour-phase-mediated activity of 175 essential oils (EOs) and 37 EO components. (sciencedaily.com)
To confirm this popular use, ethanol extract from leaves of G. globosa L. was prepared by maceration and analyzed by some phytochemical and biological assays, including cardiovascular activity. (researchgate.net)

The search for new active drugs that can alleviate or cure different diseases is a constant challenge to researchers in the biological area and to the pharmaceutical industry. (scielo.br)

Can the 'standard deviation' or the assay itself be calculated from these data, as if they were direct, repeated measurements? (physicsforums.com)
the precision of the estimate should always be determined, using the data of the assay. (pharmacologycorner.com)
It was found that the assay provides qualitative data for ranking the cleavage speeds of different linkers. (aalto.fi)

Hi everyone, I have a question about the error on certain biological results. (physicsforums.com)
When the absolute amounts of standard used in the assay are known, the results of the assay can be used to estimate the amount - in absolute units - of biologically active material contained in the unknown preparation. (pharmacologycorner.com)
Reported results for all assays meet the Division of Laboratory Sciences' quality control and quality assurance performance criteria for accuracy and precision, similar to the Westgard rules (Caudill, et al. (cdc.gov)
The results of a study that evaluated the Chinese-hamster V79 cell metabolic cooperation assay were presented. (cdc.gov)

How to find a Gold Standard assay and develop a validation plan against it? (complianceonline.com)
The low relative standard deviation values of inter-day and intra-day assays highlighted the good reproducibility of the proposed method for assay of nevirapine. (bgu.ac.il)
The standard assay couples IP to immunoblotting (IP/IB), a procedure severely limited as it is not easily scaled for high-throughput analysis. (biomedcentral.com)
Antinuclear antithrombin, protein C, protein S or pres- antibodies were investigated with standard- ence of antiphospholipid antibodies, are ized enzyme-linked immunosorbent assay common in patients with retinal vein occlu- sions and may contribute to the etiology of (ELISA). (who.int)

The purpose of the present study was to compare the amounts of vitamin A in sweet potato flour with that of carrot flour and dehydrated carrots by using the biological assay method. (unt.edu)

Ordinarily, the relationship between changes in behavior of the indicator and differences in drug dose - (a dose-effect curve) - must be determined as a part of each assay. (pharmacologycorner.com)
Bioanalytical assays are vital at all stages of the drug development process. (uscalifornia.com)
Researchers may not perform complete assay validation during early drug discovery studies. (uscalifornia.com)
A completely validated bioanalytical assay is crucial as the drug compound is tested in human subjects. (uscalifornia.com)

ENVI Assay System biodefence tests provide military and civil defence officers with a fast, simple, and reliable solution. (j3seven.com)

The assay usually involves some form of automation or a way to conduct multiple assays at the same time using sample arrays. (bvsalud.org)

Purpose: Biological and/or physical assays for retrospective dosimetry are valuable tools to recover the exposure situation and to aid medical decision making. (lu.se)

Life scientists have often taken a manual, one-factor-at-a-time approach to experimentation that omits critical insights into how simultaneous changes in multiple factors can affect the biological outcomes. (jmp.com)

Of notable importance in this respect is the theory of Generalized Linear Models with which a wide range of dilution assays can be modelled. (wikipedia.org)
Moreover, because of the wide range of applications of the IP assay, quick and high-throughput ways to determine the success of an IP are necessary. (biomedcentral.com)
Similarly, a range of diverse biological assays are available. (scielo.br)

These requirements may need simple assay development, primarily focusing on screening and assessing lead compounds. (uscalifornia.com)

how to slowly get the new technology up and running, validating the quality system, equipment and the assay itself. (complianceonline.com)

In addition, a human liver S9 assay designed for full ADCs was investigated for the feasibility of studying linker-payload constructs by themselves. (aalto.fi)

This study establishes a new link between actual human internal POP levels and the endocrine active dose in vitro, applying partitioning-controlled dosing from silicone to the H295R steroidogenesis assay: (1) Measured concentrations of POPs in silicone breast implants were taken from a recent study and silicone disks were loaded according to these measurements. (nih.gov)

In a direct dilution assay the amount of dose needed to produce a specific (fixed) response is measured, so that the dose is a stochastic variable defining the tolerance distribution. (wikipedia.org)

The authors conclude that since the promotion of tumors is complex and largely unknown, more experimentation is needed to understand the role of cell to cell communication and the usefulness of the metabolic cooperation assay. (cdc.gov)

Dilution assays can be direct or indirect. (wikipedia.org)

Anatomy3

Organisms2

Chemicals and Drugs6

Analytical, Diagnostic and Therapeutic Techniques and Equipment8

Phenomena and Processes12

Anthropology, Education, Sociology and Social Phenomena1

Information Science4

Vitro assays4

Cytotoxicity3

Molecules4

Potency1

Bioassay4

Vivo1

Quantify1

Biomarkers1

Diagnostic2

Particles2

Specificity1

MeSH1

Processes2

Validate7

Sensitivity1

Analytical2

Detection3

Mathematical1

Statistical2

Clinical7

Reproducible2

Molecular biology1

Chemical2

Test2

Research8

Cells2

Threats2

Candidates1

Samples2

Activity3

Search1

Data3

Results4

Standard4

Method1

Drug4

Tests1

Automation1

Exposure1

Time1

Range3

Development1

System1

Full1

Human1

Amount1

Cell1

Direct1