Autoantibodies centromere. Medical search. Frequent questions

Autoantibodies against nuclear antigens were detected a mean (±SD) of 5.6 ± 4.7 years before the onset of symptoms and 8.7 ± 5.6 years before diagnosis in 63% of the individuals who subsequently developed SLE. (nih.gov)
Autoantibodies against nuclear antigens were detected in individuals who developed SLE several years before the onset of symptoms and diagnosis. (nih.gov)
Anti-centromere antibodies were mainly anti-CENP-B. ELISAs for extractable nuclear antigens and the assays for dsDNA were negative. (biomedcentral.com)
Results further suggest significant promise for the development of panels of breast cancer-specific, premalignant-phase autoantibodies, as well as studies on the autoantibody response to tumor associated antigens in the pathogenesis of cancer. (biomedcentral.com)
This process is thought to be mediated by immune cells and no role is considered for the autoantibody response to tumor associated antigens [TAAs]. (biomedcentral.com)
Beads assay could detect more autoantibodies than ELISA, suggesting autoantibodies target to antigens with native conformation. (elsevierpure.com)
Although at least 9 proteins are known to be associated with the centromere complex, CENP-B is normally considered to be the major centromere antigen, since autoantibodies to this protein were found to be present at high titres in all ACA sera while titres of antibodies directed at two other centromere antigens (CENP-A, CENP-C) were often much lower. (arodia.com)
Testing for autoantibodies against centromeric antigens ( Figure 1 ) and the AMA may help to define the prognosis of both autoimmune disorders. (scientificarchives.com)
Testing of IgG autoantibodies to human cellular antigens was performed by the HEp-2 cell immunofluorescence assay using slides from INOVA Diagnostics, San Diego, CA (Cat # 508100) following the manufacturer's instructions and evaluated primarily using their NOVA View system. (cdc.gov)
Human ANA screen ELISA kit is designed for screening in vitro quantitative concentrations of IgG autoantibodies to nuclear antigens (antinuclear antibodies, ANA screen) in human serum or plasma. (elisakits.co.uk)
A mixture of purified antigens SS-A 60, SS-A 52, SS-B, RNP-70, Sm, RNP/Sm, Scl-70, Centromere B and Jo-1 is coated on to microwells. (elisakits.co.uk)
Systemic sclerosis (SSc) is a clinically heterogeneous autoimmune disease characterized by mutually exclusive autoantibodies directed against distinct nuclear antigens. (stanfordhealthcare.org)
Mono- or polytopic, complex autoimmune disease (see also autoantibodies ) with a predominantly polygenic disposition characterised by an incorrect elimination of extracellular antigens. (altmeyers.org)
Different autoantibodies can be detected in patients infected with COVID-19.ObjectivesTo explore autoantibodies related to rheumatic diseases after COVID-19 infection.MethodsNinety-eight inpatients were tested for antinuclear antibodies (ANA), antibodies to extractable nuclear antigens(ENA), anti-neutrophil cytoplasmic antibodies（ANCA), anticardiolipin antibodies,a-β2GPI (IgG/IgM). (bvsalud.org)

It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies. (wikipedia.org)
Of the patients with positive autoantibodies, 60.6% (80/132) were diagnosed as PBS and 1.5% (2/132) were diagnosed as AIH (positive anti-LC-1 and anti-LKM1 antibodies). (unboundmedicine.com)
Centromere protein C is a target of autoantibodies in Sjögren's syndrome and is uniformly associated with antibodies to Ro and La. (jrheum.org)
Auto-antibodies, like anti-Scl 70, centromere and PM-Scl, help categorize pediatric patients with scleroderma, like adults. (scleroderma.org)
The most sensitive autoantibodies were ANA, Ro/SSA and dsDNA, with the highest predictive OR being for anti-dsDNA antibodies. (nih.gov)
It is well known that autoantibodies (such ANAs in SLE and rheumatoid factors and anti-cyclic citrullinated peptides antibodies in RA) can be detected many years before the onset of these ADs [ 14 , 15 ]. (biomedcentral.com)
The carboxyl-terminal fragment of CENP-B contains a major epitope for anti-centromere antibodies (ACA). (bath.ac.uk)
Objectives Recent evidences have revealed that anti-SSA/SSB antibodies, the major autoantibodies in Sjögren's syndrome (SS), are produced in salivary glands. (elsevierpure.com)
Results A total of 256 lesion antibodies were generated, and 69 autoantibodies including 24 ACAs were identified among them. (elsevierpure.com)
The target profiling of serum ACA from 269 patients with SS, systemic sclerosis (SSc), primary biliary cirrhosis (PBC) and healthy controls revealed that ACA-positive patients have antibodies against various sites of centromere complex regardless of disease. (elsevierpure.com)
Subsequent studies confirmed that anti-centromere antibodies (ACA) were most often found in patients with a limited form of systemic sclerosis previously referred to as CREST syndrome. (arodia.com)
Anticentromere antibodies (ACA) are considered an important diagnostic marker of scleroderma or systemic sclerosis (SSc), being CENP-B the major centromere auto-antigen recognized by sera from SSc patients [ 1 ]. (scientificarchives.com)
Further, autoantibodies to Nt-CENPB in patients with scleroderma and AMA positive were found in a Spanish population [ 12 ], and a recent report in a Moroccan population, SSc patients with positive Nt-CENPB antibodies also showed reactivity against AMA-M2 antibodies [ 13 ]. (scientificarchives.com)
Thus, the co - appearance of anti-centromere antibodies with others in SSc patients at different stages of disease may suggest some putative significance for the evaluation and development of this disorder [ 14 ]. (scientificarchives.com)
Anti-centromere antibodies in a large cohort of systemic sclerosis patients: comparison between immunofluorescence, CENP-A and CENP-B ELISA. (scientificarchives.com)
Antinuclear antibodies (ANAs) were determined using indirect immunofluorescence while other autoantibodies were determined by solid-phase assays. (bmj.com)
They have a number of different subtypes which include anti-histone, anti-sp100, anti-centromere, anti-Scl-70, anti-Ro, anti-La, anti-nRNP, anti-Sm and anti-dsDNA antibodies. (elisakits.co.uk)
The minimum detection sensitivity level of IgG autoantibodies to human anti-nuclear antibodies (ANA, antinuclear antibodies) using current ANA ELISA kit was diagn. (elisakits.co.uk)
Tan EM, Rodnan GP, Garcia I, Moroi Y, Fritzler MJ, Peebles C. Variety of antinuclear antibodies in intensifying systemic sclerosis: anti\centromere antibody and its own romantic relationship to CREST symptoms. (sicollaborative.org)
Test performance in systemic sclerosis: anti-centromere and anti-Scl-70 antibodies. (biovendor.group)

Both AMA and ACA present discrete autoantibody populations which may coexist in the same patient, although the coexistence of primary biliary cirrhosis and systemic sclerosis is rare [ 8 ]. (scientificarchives.com)
Sakata K, Kaneko Y, Yasuoka H, Yamaoka K, Takeuchi T. Association of Radiographic Findings in Hand X-Ray with Autoantibodies in Patients with Systemic Sclerosis [abstract]. (acrabstracts.org)
Autoantibodies in systemic sclerosis. (sicollaborative.org)
Autoantibodies in systemic sclerosis and fibrosing syndromes: clinical indications and relevance. (biovendor.group)
Bunn CC, Denton CP, Shi-Wen X. Anti-RNA polymerases and other autoantibody specificities in systemic sclerosis. (medibas.se)

52 and 60 kDa), anti-Sjögren's syndrome antigen B, anti-Smith antibody, ribonucleoprotein, scleroderma, anti-histidyl-tRNA synthetase antibody, double-stranded DNA (dsDNA), centromere protein B and histones were analysed using the AtheNA Multi-Lyte ANA II Plus Test System on a Bio-Plex Array Reader (Luminex200). (nih.gov)
This study aims to clarify overall of autoantibody production at lesion site, including anti-centromere antibody (ACA)-positive SS. (elsevierpure.com)
Twenty-six patients (26%) were positive for anti-Scl-70 antibody, 54% for anti-centromere antibody, 6% for anti-RNA polymerase III antibody, and 6% for anti-U1RNP antibody. (acrabstracts.org)
In the EA population, the HLA-DPB1*13:01 and HLA-DRB1*07:01 alleles were more strongly associated with antitopoisomerase (ATA) and anticentromere antibody-positive subsets of SSc, respectively, than with overall SSc risk, emphasizing the importance of HLA in defining autoantibody subtypes. (stanfordhealthcare.org)
More recently, we found that embryos cultured with anti-CENP-A (centromere protein-A) antibody had significant growth impairment and/or high mortality [ 12 ]. (biomedcentral.com)

Centromere protein B also known as major centromere autoantigen B is an autoantigen protein of the cell nucleus. (wikipedia.org)
In humans, centromere protein B is encoded by the CENPB gene. (wikipedia.org)
Centromere protein B is a highly conserved protein that facilitates centromere formation. (wikipedia.org)
This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. (wikipedia.org)
Centromere protein B is a potential biomarker of small-cell lung cancer. (wikipedia.org)
Entrez Gene: centromere protein B". Sugimoto K, Yata H, Himeno M (July 1993). (wikipedia.org)
Rodríguez-Bayona et al found autoantibodies to centromere passenger protein INCENP, a protein responsible for chromosomal segregation and mitosis regulation, in one patient with GLPLS. (medscape.com)
Autoantibody-secreting cells in salivary gland tissue were identified by immunohistochemistry using green fluorescent protein-Autoantigen fusion proteins. (elsevierpure.com)
ACA recognises centromere â € complex' rather than individual protein, and this feature is common among patients with SS, SSc and PBC. (elsevierpure.com)
Using HLA a/ß allelic heterodimers, we bioinformatically predicted immunodominant peptides of topoisomerase 1, fibrillarin, and centromere protein A and discovered that they are homologous to viral protein sequences from the Mimiviridae and Phycodnaviridae families. (stanfordhealthcare.org)
Scl 70 autoantibodies from scleroderma patients recognize a 95 kDa protein identified as DNA topoisomerase I. Chromosoma. (biovendor.group)
We divided mice into three groups: human recombinant centromere protein-A (human CENP-A, HA) and complete Freund's adjuvant (CFA) were used to immunize mice for the study group (HA + CFA), and mice injected with CFA (CFA group) or saline (Saline group), respectively, served as controls. (biomedcentral.com)

In the (B6.H2 z × NZB)F 1 × NZB backcross, MHC was an additional genetic contribution that interacted with Nba2 in the production of autoantibodies and the development of nephritis. (johnshopkins.edu)
Systemic lupus erythematosus (SLE) remains the prototypical autoimmune systemic disease in which hyperactivity of the immune system and production of autoantibodies lead to a variety of symptoms including chronic pain, arthralgia, fatigue, morning stiffness and, most importantly, damage in key organs including the kidney and central nervous system. (bmj.com)

Human Epithelial type 2 (HEp-2) images are important in detecting the antinuclear autoantibody (ANA) in diagnosis of autoimmune disease in human body. (usm.my)

Nba2 was linked with elevated serum levels of multiple autoantibodies, including a variety of antinuclear Abs (anti-dsDNA, antichromatin and anti-histone) and autoantibodies to gp70, in both backcrosses. (johnshopkins.edu)
IgG autoantibody testing of a subsample of stored serum from phase 1 (1988-91) NHANES III participants was conducted to estimate the prevalence and specificities of selected autoantibodies in the US population. (cdc.gov)
QUANTA Lite assays enable semi-quantitative detection of autoantibodies in human serum for a variety of diseases. (werfen.com)
We did not find a statistical difference in the above results.ConclusionWe have not found a correlation between SARS-CoV-2 and serum autoantibodies of rheumatic immune diseases. (bvsalud.org)

The prevalence of abnormal findings in X-ray and the associations with clinical features, SSc subtypes and autoantibodies were analyzed. (acrabstracts.org)
HLA and autoantibodies define scleroderma subtypes and risk in African and European Americans and suggest a role for molecular mimicry. (stanfordhealthcare.org)

Autoantibodies are frequently detected in the presence of autoimmune liver diseases (ALD) [autoimmune hepatitis (AIH), primary biliary cirrhosis (PBC), primary sclerosing cholangitis (PSC)] and are widely used to classify the disease clinically. (unboundmedicine.com)

Fourteen of the patients (10.6%) with centromere, nuclear membrane (NM), multiple nuclear dot (MND) staining patterns and elevated liver enzymes could not be diagnosed as a specific disease and were followed up. (unboundmedicine.com)
ANA patterns were detected in 32.6% (43/132) of the patients (NM 9.1%, centromere 9.1%, MND 6.8%, respectively). (unboundmedicine.com)
The characteristics, laboratory and radiological findings of the patients with isolated AMA positivity alone (Group 1) and patients with multiple patterns/autoantibodies (Group 2) were compared. (unboundmedicine.com)
In patients with multiple patterns/autoantibodies (Group 2), the presence of cirrhosis and liver heterogeneity were found to be higher than Group 1 (p= 0.049). (unboundmedicine.com)
There is no clear information in real-life about the reasons for referral, the presence of clinical findings of autoimmune diseases, the frequency of patterns of autoantibodies, and specific capillaroscopic findings. (upb.edu.co)

Autoantibody to centromere (kinetochore) in scleroderma sera. (upb.ro)

The more accurate measurement of SSc-specific autoantibodies such as topoisomerase 1, centromere and RNA polymerase has recently allowed a more precise subclassification of SSc with implications for treatment and prognosis. (medscape.com)
Evaluation of autoantibodies against RNA polymerases using immunoaffinity purified RNA polymerase I, III and II antigen within an enyme\linked immunosorbent assay. (sicollaborative.org)

Base for the balance of both types of autoantibodies, a distinct weight could be given to the anti-CENPB and anti-mitochondria specificities in the SS classification criteria and the development of PBC in SSc patients [ 16 , 17 ]. (scientificarchives.com)

The aim of this study was to investigate the contribution of autoantibodies for the diagnosis of ALD and the identification of other accompanying systemic autoimmune rheumatic diseases (SARD). (unboundmedicine.com)
Detection of ALD-related autoantibodies in patients diagnosed with SARD can provide early diagnosis of these patients. (unboundmedicine.com)
These autoantibodies guide both diagnosis and prognosis as in PBC. (unboundmedicine.com)
We report on a small-cell lung cancer case that was heralded by the presence of CENP-B specific anti-centromere autoantibodies (ACA) detected well before the diagnosis of cancer. (ucy.ac.cy)

Interestingly, higher prevalence of both PBC-associated autoantibodies and PBC in the Japanese SSc population was found than in the Caucasian SSc population [ 10 ]. (scientificarchives.com)
Various radiographic abnormalities are observed, especially in hands, however, the detailed prevalence and the association with autoantibodies are still unclear. (acrabstracts.org)

The analysis of antinucleolar and antinuclear autoantibodies of scleroderma by radioimmuno\precipitation assays. (sicollaborative.org)

Autoantibodies to centromeric proteins in the sera of patients with scleroderma were first described in 1980. (arodia.com)

Using new ELISA-based technology (sometimes called EIA for enzyme immunoassay) to screen for multiple extractable nuclear antigen (ENA) markers, centromere and double-stranded DNA (dsDNA) has automated testing at Baptist Medical Center and greatly improved the PPV for our ANA screen. (clinlabint.com)

Autoantibodies have a central role in systemic lupus erythematosus (SLE). (nih.gov)
These autoantibodies are also often detected in sera from patients with Raynaud's phenomenon and occasionally in other rheumatic diseases such as systemic lupus erythematosus, Sjögren's syndrome, and rheumatoid arthritis. (arodia.com)

The determination of autoantibody titers from 1:80 to 1:1280 was performed by serial dilution on samples that showed a 3+ or greater nuclear and/or cytoplasmic immunofluorescence pattern. (cdc.gov)

this suggests that the process triggering autoantibody formation starts in the pre-malignant phase and that future studies using validated autoantibody panels may allow detection of breast cancer risk in asymptomatic women. (biomedcentral.com)

Anticentromere autoantibodies. (scientificarchives.com)

The presence of autoantibodies preceding disease onset by years has been reported both in patients with SLE and in those with rheumatoid arthritis, suggesting a gradual development of these diseases. (nih.gov)
The objective of this work was to demonstrate that autoantibodies in breast cancer sera are not epiphenomena, and exhibit unique immunologic features resembling the rheumatic autoimmune diseases. (biomedcentral.com)
Our studies on autoantibodies in malignancies strongly suggested that cancer sera exhibit immunologic features that are common in the rheumatic autoimmune diseases [ADs]. (biomedcentral.com)

Puffy fingers, Raynaud's phenomenon, positive SScspecific autoantibodies and capillaroscopy changes indicate early SSc, even if skin thickening proximal to the metacarpophalangeal joints is not present. (medscape.com)

0.05 for both) while anti-centromere was inclined to associate with calcinosis (p=0.08). (acrabstracts.org)

Therefore, we sought to identify autoantibodies in a northern European population predating the onset of symptoms of SLE and their relationship to presenting symptoms. (nih.gov)
The time predating disease was shorter and the number of autoantibodies was greater in those individuals with serositis as a presenting symptom in comparison to those with arthritis and skin manifestations as the presenting symptoms. (nih.gov)

An autoantibody found in 80% of people with Rheumatoid Arthritis , a common form of arthritis which affects the joints in the hands and wrists. (requestatest.com)

The distinctive autoantibody profile detected in BC sera is the expression of tumor immunogenicity. (biomedcentral.com)

Autoantibodies have been reported to bind to at least 3 different epitope regions of CENP B however the 60 amino acid C-terminal region appears to constitute the major autoimmune epitope. (arodia.com)
They are a specific class of autoantibodies which can bind and destroy certain structures within the nucleus of the cells. (elisakits.co.uk)

Autoantibody tests were found to be negative in 461 (77.7%) of 593 patients (mean age= 53.3 ± 15.6, age range= 18-90), and were positive in 132 (22.3%) (86.4% female) of the patients. (unboundmedicine.com)
The most common pattern was the AMA staining pattern (34.8%, 46/132) among the autoantibody positive patients. (unboundmedicine.com)
In the ENA profile results of these patients, the most common profile detected was anti-Ro-52 (65.9%, 27/41), followed by anti-SSA (34.1%, 14/41), anti-SSB (22.0%, 9/41) and anti-CENP-B (12.2%, 5/41) autoantibodies , respectively. (unboundmedicine.com)
In our study, 87.5% (70/80) of the patients diagnosed as PBS were found to have AMA positivity and 12.5% (10/80) of them had ANA positivity (such as NM, CNN, centromere). (unboundmedicine.com)
Autoantibodies were detected in virtually all patients with breast cancer, predominantly of the IgG1 and IgG3 isotypes. (biomedcentral.com)

Our objective was to clarify the characteristics of radiographic findings in hand X-ray and their association with autoantibodies in SSc. (acrabstracts.org)

Both backcrosses were examined by interval mapping for quantitative trait loci linked with autoantibody and total Ig production. (johnshopkins.edu)

This locus, designated Nba2 for New Zealand Black autoimmunity 2, was found to colocalize in both (NZB × SM/))F 1 × NZW and (B6.H2 z × NZB)F 1 × NZB backcrosses, and was most likely situated between 92 and 97 cM from the centromere. (johnshopkins.edu)

CENP-B specific anti-centromere autoantibodies heralding small-cell lung cancer. (wikipedia.org)
CENP-B may therefore function as a trans-acting factor that regulates the formation or centromere-specific chromatin on the α-satellite DNA repeat at the nucleosome assembly level. (arodia.com)
The task force proposes the term "interstitial pneumonia with autoimmune features" (IPAF) and offers classification criteria organised around the presence of a combination of features from three domains: a clinical domain consisting of specific extra-thoracic features, a serologic domain consisting of specific autoantibodies, and a morphologic domain consisting of specific chest imaging, histopathologic or pulmonary physiologic features. (ersjournals.com)
Skin biopsy (looking for eosinophil infiltration, increased mucin or amyloid deposition), SSc-specific autoantibodies or paraproteins in blood and a careful medical history and system screening will exclude nonscleroderma conditions. (medscape.com)

Taken together, these data suggest a possible link between HLA alleles, autoantibodies, and environmental triggers in the pathogenesis of SSc. (stanfordhealthcare.org)

The type of autoantibodies was also associated with the radiographic findings. (acrabstracts.org)

Anatomy27

Organisms10

Diseases28

Chemicals and Drugs52

Analytical, Diagnostic and Therapeutic Techniques and Equipment14

Phenomena and Processes58

Technology, Industry, Agriculture1

Information Science3

Antigens14

Antibodies20

Systemic sclerosis5

Antibody5

Protein12

Production of autoantibodies2

Autoimmune disease1

Serum4

Subtypes2

Primary biliary c1

Patterns5

Kinetochore1

Polymerase2

Specificities1

Diagnosis4

Prevalence2

Assays1

Centromeric1

Double-stranded1

Lupus2

Immunofluorescence1

Detection1

Anticentromere1

Diseases3

Raynaud's1

Calcinosis1

Presenting symptoms2

Rheumatoid1

Tumor1

Bind2

Patients5

Clarify1

Quantitative1

Found1

Specific4

Pathogenesis1

Findings1