• The contribution of specific genes or proteins to the bacteria's ability to invade cells can be easily assayed using this method. (wikipedia.org)
  • Thus, HERA is a novel cellular assay that can be used to predict how FcRn-binding proteins are rescued from intracellular degradation. (nature.com)
  • Proteomic approaches have proven powerful at identifying large numbers of proteins, but there are fewer reports of functional characterization of proteins in biological tissues. (hindawi.com)
  • Following activation by agonists such as thrombin, platelets release storage granules and membrane vesicles that contain prothrombotic (e.g., fibrinogen), mitogenic (e.g., platelet derived growth factor), immunomodulatory (e.g., neutrophil-activating peptide 2), and adhesive (e.g., platelet endothelial cell adhesion molecule) proteins. (hindawi.com)
  • Interleukin 1 (IL-1) is a name that designates two proteins, IL-1 alpha and IL-1 beta, which are the products of distinct genes, but which show approximately 25% amino acid sequence identity and which recognize the same cell surface receptors. (rndsystems.com)
  • Fusion Proteins offer an important biological assay for direct protein-to-protein interactions. (thermofisher.com)
  • Fluorescent reporter proteins in the Drosophila model system offer a degree of specificity that allows monitoring cellular and biochemical phenomena in vivo, such as autophagy, mitophagy, and changes in the redox state of cells. (intechopen.com)
  • Therefore, this chapter will show exciting results of the group using fluorescent proteins and nanocrystals in biological systems. (intechopen.com)
  • The fact that many Rho family effector proteins will specifically recognize the GTP bound form of the protein has been exploited experimentally to develop a powerful affinity purification assay that monitors RhoA protein activation. (cytoskeleton.com)
  • Active caspases can then cleave many proteins, including poly-ADP ribose polymerase (PARP), other caspases and other protein substrates en masse. (bdbiosciences.com)
  • Indeed, platelet releasate has previously been shown to induce endothelial cell permeability, endothelial cell chemotaxis, and corneal epithelial cell proliferation in cellular assays [ 7 - 9 ]. (hindawi.com)
  • We also showed that dose-dependent induction of proliferation was connected with changes in the expression of MKI67, CCND1 and CCNE1 genes in well- and poorly differentiated cancer cells. (spandidos-publications.com)
  • Our study revealed that Ang II influences EC cells in terms of cancer-related processes, and is responsible for increased proliferation, reduction in apoptosis, increased mobility and modulation of adhesion potential. (spandidos-publications.com)
  • Therefore, various biologically active peptides such as angiotensin, affecting cell proliferation, have become a new area of study in endometrial cancer research. (spandidos-publications.com)
  • Recently, a number of studies have shown that Ang II plays an important role in proliferation, invasiveness and migration of tumour cells, alteration of expression of cancer-related genes, as well as in physiological tissue remodelling. (spandidos-publications.com)
  • indicated that ATMs colocalized with T cells in lymphoid clusters within adipose tissue and may act as APCs, which express high levels of MHCII and also costimulatory molecules and process and present antigens to induce CD4+ T-cell proliferation and activation in adipose tissue of obese mice (29, 68, 105). (exposed-skin-care.net)
  • showed that adipose tissue from obese mice induced proliferation of splenic CD8+ T cells, indicating a CD8+ T cell-activating environment in obese adipose tissue (31). (exposed-skin-care.net)
  • 2002 ). As a pivotal biochemical cascade, the MAPK signaling pathway is involved in diverse fundamental events in cells and signal transduction pathways, namely, differentiation, proliferation, apoptosis, activation of other specific regulatory factors, acute responses to hormones, and developmental alteration of organisms (Pearson et al. (frontiersin.org)
  • These cells allow different types of assays to be performed, covering the T cell-mediated immune response from antigen uptake to T cell proliferation. (ddw-online.com)
  • A T cell activation and proliferation (i.e. (ddw-online.com)
  • These are recognised by T cells, resulting in proliferation. (ddw-online.com)
  • The antigen priming of CD4+ T cells by mature MoDCs leads to the proliferation of CD4+ T cells in in-vitro PBMC assays. (ddw-online.com)
  • The Hippo pathway regulates organ size and tissue homeostasis by controlling cell proliferation and apoptosis. (bvsalud.org)
  • Furthermore, POGLUT2 knockdown of breast cancer cells was established, followed by in vitro biological function assays and in vivo tumor growth study. (frontiersin.org)
  • Usually, human primary immune cells are employed for in vitro biological assays for immunogenicity prediction, and though they are generally obtained from healthy donors, samples from specific subpopulations of patients can be of interest in the study of disease-specific immunogenic reactions. (ddw-online.com)
  • A TdT-mediated dUTP nick-end labeling (TUNEL) assay was used to identify cell apoptosis. (frontiersin.org)
  • Apoptosis is an organized process that signals cells to self-destruct for cell renewal or to control aberrant cell growth. (bdbiosciences.com)
  • As cells become damaged or are no longer needed, they undergo apoptosis or programmed cell death, a normal physiological process that occurs during embryonic development and tissue homeostasis. (bdbiosciences.com)
  • Apoptosis controls the orderly death of damaged cells, whereas necrosis occurs as a result of tissue damage, causing the loss of both damaged and surrounding cells. (bdbiosciences.com)
  • However, some cell types do not display characteristic features of apoptosis. (bdbiosciences.com)
  • In those cases, multiple aspects of apoptosis might need to be analyzed to confirm the mechanism of cell death. (bdbiosciences.com)
  • Methods for detecting apoptosis or dead cells (viability) by cell preparation type. (bdbiosciences.com)
  • During apoptosis, PS translocates to the outer leaflet of the plasma membrane and can be detected by flow cytometry and cell imaging through binding to fluorochrome-labeled annexin V when calcium is present. (bdbiosciences.com)
  • With the addition of these new formats, more complex assays can be developed to look at apoptosis within heterogeneous cell subsets. (bdbiosciences.com)
  • Radio-frequency dose-dependent apoptosis, necrosis and cell death monitored by annexin V-BD Horizon™ V450. (bdbiosciences.com)
  • Radio-frequency dose-dependent apoptosis, necrosis and cell death monitored by annexin V-BD Horizon™ V450 in pancreatic carcinoma cell lines treated with a low dose of cetuximab targeted gold nanoparticles. (bdbiosciences.com)
  • In the past decade, super-resolution fluorescence microscopy has revolutionized biological research by enabling the study of biological functions down to the molecular scale. (uni-goettingen.de)
  • SuFIDA (surfaced-based fluorescence immuno digital assay) is the combination of the proven principle of a sandwich ELISA and digital readout using highly sensitive fluorescence microscopy. (fz-juelich.de)
  • Human peripheral blood mononuclear cells (PBMCs) treated with LPS for 24 hours were stained with Mouse Anti-Human IL-1a/IL-1F1 Membrane Form PE-conjugated Monoclonal Antibody (Catalog # FAB200P, filled histogram) or isotype control antibody (Catalog # IC002P , open histogram). (rndsystems.com)
  • We cannot exclude the possibility that whole blood acquired for use in biological warfare or bioterrorist assay sensitivity could be improved by changing protocol attacks. (cdc.gov)
  • The SuFIDA Innovation Lab develops digital assays for various biomarkers with highly specific single-molecule sensitivity. (fz-juelich.de)
  • First of all, let's not confuse the assay sensitivity with its clinical sensitivity. (medscape.com)
  • The assay sensitivity is what we do in the lab with limiting dilution of reagents, and we say, what's the least amount of molecules we can detect? (medscape.com)
  • An alternative invasion assay is the differential immunostaining assay, based on the binding of antibodies to bacteria before and after invasion. (wikipedia.org)
  • The antibodies emit fluorescent, colored light, and the results of this assay are viewed under the microscope. (wikipedia.org)
  • Attana has filed an international patent application with claims for methods for assessing the neutralizing potential of antibodies in biological samples. (cision.com)
  • Using a multidisciplinary approach, we examined the mechanism of binding of compound 5a to the nsp16 cryptic pocket and developed 5a derivatives that inhibited nsp16 activity and murine hepatitis virus replication in rat lung epithelial cells but proved cytotoxic to cell lines canonically used to examine SARS-CoV-2 infection. (bvsalud.org)
  • PBMC, DC:T) assay makes use of APC surface expression of specific antigens, eg. (ddw-online.com)
  • BD Biosciences tools use multiple methodologies including flow cytometry , bioimaging and microscopy (for live and fixed cell analysis) as well as ELISA , IHC , western blot and spectrofluorometry. (bdbiosciences.com)
  • In obese humans, adipocyte-secreted CCL20 may contribute to the deposition of Compact disc4+ helper and Compact disc8+ cytotoxic T lymphocytes within adipose tissues, possibly via connections with CCR6 which was upregulated on T cells in obese adipose tissues (100). (exposed-skin-care.net)
  • However, the main element substances that mediate T cell infiltration into adipose tissues in maturing remain to become discovered. (exposed-skin-care.net)
  • Activation of Typical T Cells in Adipose Tissues Compact disc4+ Purpureaside C T Cell Activation TCRs recognize the current presence of a particular antigen by binding to brief peptide sequences in the antigen that's shown on APCs. (exposed-skin-care.net)
  • further defined that mostly huge adipocytes from obese adipose tissues exhibited an increased expression degree of MHCII substances and acted as APCs to activate Compact disc4+ T cells to secrete IFN- (103). (exposed-skin-care.net)
  • Increased levels of POGLUT2 were found in multiple types of cancer tissues and cell lines. (frontiersin.org)
  • Table 7-1 summarizes the analytical methods used to detect malathion and its metabolites as well as cholinesterase activity in biological tissues and fluids. (cdc.gov)
  • It was necessary to perform serum assays within 48 hours of patient exposure since malathion is quickly hydrolyzed in human tissues. (cdc.gov)
  • Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
  • [12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
  • The order shows a market acceptance for our technology and capability to characterize interactions with cells, tissues, viruses etc it also provides a value of our application knowledge and the advises we can provide. (cision.com)
  • This may happen if the permeability of the cells somehow increased during the assay, sometimes due to poor handling of the cells. (wikipedia.org)
  • Thus temporally increased cell permeability enables extracellular molecules with otherwise hampered transmembrane transport to enter the cells. (hindawi.com)
  • A use case from current pharmaceutical research addressing centrosomal clusters in cancer cells was successfully optimized for STED microscopy. (uni-goettingen.de)
  • Activated by stress, p38 MAPKs regulate vital cellular responses such as migration, contraction, cytokine production, and death in macrophages and myocardial and endothelial cells (Marber et al. (frontiersin.org)
  • Isolated cellular subsets can allow better control in terms of assay set-up, but care must be taken to ensure minimal cellular damage, maximum yield, and standardised processing. (ddw-online.com)
  • This leads to the loss of cellular structure and function, and ultimately results in cell death. (bdbiosciences.com)
  • Environmental conditions, such as pH level and temperature, can also be assayed for their effect on invasiveness. (wikipedia.org)
  • Since then, Attana has developed proprietary label free biosensors for biochemical, crude, sera, and cell-based assays and the Attana Virus Analytics (AVA) platform, a proprietary in vitro diagnostics (IVD) tool. (cision.com)
  • Smallpox Vaccination antigens are added to blood cells, allowing the simultane- ous analysis of both major histocompatibility complex class I and II restricted T-cell responses (7). (cdc.gov)
  • 2 years orthopoxviruses is considered, this rapid assay that uses vaccinia antigens could identify recently exposures. (cdc.gov)
  • the assay can rorism or biological warfare represents a risk for epidemic potentially be used as a routine diagnostic assay. (cdc.gov)
  • Expertise and capability in functional genomics and DNA based assay design can be applied to numerous disciplines, research interests and diagnostic test development in the food or animal testing areas. (dcu.ie)
  • Also, growing demand for rapid and precise measurement of biomolecules, increased use of Immunofluorescence Assay for diagnostic and research purposes, increased R&D activities and advanced technology are the key factors for the market to grow. (medgadget.com)
  • The use of international reference materials for designating the activity of biological substances used in prophylaxis or therapy, or for ensuring the reliability of diagnostic procedures, allows comparability of data globally. (who.int)
  • Methodologically, the study showed that silicone can be used as reference partitioning phase to transfer in vivo exposure in humans (silicone implants) to in vitro assays (partition-controlled dosing). (nih.gov)
  • Neither precursor contains a typical hydrophobic signal peptide sequence and most of the precursor form of IL-1 alpha remains in the cytosol of cells, although there is evidence for a membrane-bound form of the precursor form of IL-1 alpha. (rndsystems.com)
  • These brief peptide sequences in the antigen are often presented over the cell surface area of APCs by using MHCII substances, which are necessary for activation of Compact disc4+ T cells (102). (exposed-skin-care.net)
  • MAPPS) assays use purified HLA-II peptides of the protein of interest to complement in silico identification and evaluation of the binding affinity of HLA-II peptide epitopes. (ddw-online.com)
  • BD Biosciences also offers a range of tools for caspase activity assays from individual fluorogenic peptide substrates and inhibitors, to kits, to ready-to-use assay plates. (bdbiosciences.com)
  • Biological assays and gene expression analysis were performed on three cell lines: ISH, MFE-296 and MFE-280. (spandidos-publications.com)
  • Expression, gene mutation and amplification, methylation, and copy number alteration (CNA) of POGLUT2 were evaluated using The Cancer Genome Atlas (TCGA), Cancer Cell Line Encyclopedia (CCLE), and Genotype-Tissue Expression (GTEx) databases. (frontiersin.org)
  • Enhancing HH signaling by decreasing the gene dosage of a negative regulator of the pathway, Ptch1 , decreased cell death and rescued the midface defect in both Tctn2 and Cc2d2a mutants. (elifesciences.org)
  • however, the assay had reduced sen- recur as an endemic disease, but the virus may have been sitivity. (cdc.gov)
  • It's below the assay detection limit, but they recur later. (medscape.com)
  • This GST-fusion protein can then be purified from cells via its high affinity for glutathione. (thermofisher.com)
  • The fact that the RBD region of Rhotekin has a high affinity for GTP-RhoA and that Rhotekin binding results in a significantly reduced intrinsic and catalytic rate of GTP hydrolysis make it an ideal tool for affinity purification of GTP-RhoA from cell lysates. (cytoskeleton.com)
  • All cell culture reagents were obtained from Gibco ® by Life Technologies™ (Warsaw, Poland). (spandidos-publications.com)
  • The kit contains sufficient materials for 20 assays, depending on assay setup, and includes reagents for positive and negative controls. (cytoskeleton.com)
  • This study establishes a new link between actual human internal POP levels and the endocrine active dose in vitro, applying partitioning-controlled dosing from silicone to the H295R steroidogenesis assay: (1) Measured concentrations of POPs in silicone breast implants were taken from a recent study and silicone disks were loaded according to these measurements. (nih.gov)
  • E-Screen assays for endocrine disruptor chemicals. (dcu.ie)
  • Here, we report on a novel human endothelial cell-based recycling assay (HERA) that can be used for such pre-clinical screening. (nature.com)
  • Last week four different clinical groups at one of our key customers were trained in performing biological relevant assays using primary cells. (cision.com)
  • Analysis of anticoagulants for blood-based quantitation of amyloid beta oligomers in the sFIDA assay. (fz-juelich.de)
  • The gentamicin protection assay or survival assay or invasion assay is a method used in microbiology. (wikipedia.org)
  • HeLa cells are commonly used as eukaryotic cells in the gentamicin protection assay, but other cells can be used as well. (wikipedia.org)
  • The assay is performed in plastic microtiter plates, which are commonly used in laboratories for culturing eukaryotic cells. (wikipedia.org)
  • Next the eukaryotic cells are lysed using a detergent, most commonly Triton X-100. (wikipedia.org)
  • The gentamicin protection assay is commonly used in pathogen research. (wikipedia.org)
  • Since intracellular annexin V is also exposed if the plasma membrane is compromised, a membrane-impermeant dye such as 7-AAD is commonly used to distinguish between apoptotic and dead cells to exclude the dead cells. (bdbiosciences.com)
  • It is used to quantify the ability of pathogenic bacteria to invade eukaryotic cells. (wikipedia.org)
  • Counting the colonies formed on the plates on the next day, and knowing how many bacteria were used in the beginning of the assay, enables the researcher to calculate the percentage of bacteria that were able to invade the eukaryotic cells. (wikipedia.org)
  • As the host cells rupture, the trypomastigotes are released into the lymphatics and bloodstream, through which they spread to distant sites and invade new host cells. (medscape.com)
  • CdSe/CdS magic-sized quantum dots (MSQDs) show high luminescence stability in biological systems and can be bioconjugated with biological molecules. (intechopen.com)
  • This initiative is one of the integrated components of the NIH Molecular Libraries Roadmap initiative that offers biomedical researchers access to large-scale automated high throughput screening (HTS) centers in the MLPCN, diverse compound libraries in the Small Molecule Repository (MLSMR) and information on biological activities of small molecules in the PubChem BioAssay public database. (nih.gov)
  • By implementing camera-based widefield imaging for both cell and feature detection, the throughput of the imaging platform could be considerably increased compared to an approach based solely on laser scanning. (uni-goettingen.de)
  • In response to classic stimuli produced by inflammatory agents, infections or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cells is seen. (rndsystems.com)
  • Another statement showed the preadipocyte- and endothelial cell-derived stromal-derived element-1 (CXCL12), mediated early infiltration of CD4+ T lymphocytes in obesity, which preceded the increase of macrophages in adipose cells of mice on HFD (101). (exposed-skin-care.net)
  • POGLUT2 was mainly expressed in stromal cells as verified by StromalScore, ESTIMATEScore, ImmuneScore, and Tumor purity, and POGLUT2 was positively correlated with cancer-associated fibroblasts, macrophages, monocytes, and neutrophils in the tumor microenvironment. (frontiersin.org)
  • Through its funding of the MLPCN, NIH will support the costs of assay automation, screening and optimization chemistry. (nih.gov)
  • Biological chemistry. (fz-juelich.de)
  • As for bacteria, only species susceptible to gentamicin can be assayed. (wikipedia.org)
  • Among various species, the amino acid sequence of mature IL-1 alpha is conserved 60% to 70% and human IL-1 has been found to be biologically active on murine cell lines. (rndsystems.com)
  • Moreover, overnight incubation with 10 µg/mL of the secretion antigen-specific T-cell responses could be detected in inhibitor Brefeldin-A (Sigma-Aldrich Corporation, St. exposed, but uninfected persons, as shown in those with Louis, MO). (cdc.gov)
  • Indeed, the principal adipocytes isolated from obese mice could induce antigen-specific Compact disc4+ T cell activation (58). (exposed-skin-care.net)
  • Increased levels of POGLUT2 could result in the dysregulated immune cell infiltration and tumor microenvironment and showed a significant regulatory effect on the progression of breast cancer through Notch-related signaling. (frontiersin.org)
  • Complex biological samples have also been successfully separated in multiple dimensions at the protein level. (hindawi.com)
  • The assay uses the Rho binding domain (RBD) of the Rho effector protein, Rhotekin. (cytoskeleton.com)
  • The RhoA activation assay was tested by loading the RhoA protein in cell lysates with either GTPγS or GDP. (cytoskeleton.com)
  • Titanium dioxide (TiO2) nanocrystals (NCs) have several biological applications and emit in the ultraviolet, with doping of europium ions can be visualized in the red luminescence. (intechopen.com)
  • Using such antibiotics enables us to differentiate between bacteria that succeed in penetrating eukaryotic cells and those that do not. (wikipedia.org)
  • Indeed, CD8+ T cells from mouse adipose tissue respond to cytokines and become activated and proliferate under stimulation of IL-12 and IL-18, which are mainly produced by APCs and are elevated in obese adipose tissue (44). (exposed-skin-care.net)
  • In the case of cytokine release, pro-inflammatory cytokines, such as TNFα, IFNɣ, IL-2, IL-4, IL-6, IL-8, and IL-10 are released consecutive to the co-activation of DCs and T cells. (ddw-online.com)
  • Secondary (indirect) immunofluorescence assays category includes salt split technique, antigenic mapping method and double staining method. (medgadget.com)
  • The increasing complexity and sophistication of biological/biotechnological substances used in human medicine, and the rapid growth in their volume, present a considerable challenge for regulatory authorities, especially in the developing world. (who.int)
  • The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
  • The NIH Molecular Libraries Roadmap Initiative wishes to solicit HTS assay applications from the scientific community from investigators who have the interest and capability to work with the Molecular Libraries Probe Production Centers Network (MLPCN) in support of chemical probe development. (nih.gov)
  • This FOA promotes discovery and development of new chemical probes as research tools for use by scientists in both the public and private sector to advance the understanding of biological functions and disease mechanisms. (nih.gov)
  • The primary metabolites found in biological fluids following exposure to malathion are malathion dicarboxylic acid (DCA) and malathion monocarboxylic acid (MCA). (cdc.gov)
  • The immunofluorescence assay market, by technique is sub segmented into primary (direct) immunofluorescence assays, secondary (indirect) immunofluorescence assays, and micro immunofluorescence. (medgadget.com)
  • studies indicated that RANTES is Purpureaside C an adipokine that can be produced by adipocytes and takes on an important part in T cell migration, suggesting a potential part of the RANTES/CCR5 axis in adipose T cell build up in obesity (24). (exposed-skin-care.net)
  • The WHO Expert Committee on Biological Standardization reviews developments in the field of biological substances, which include vaccines, blood products and biological therapeutics, and recommends procedures to assure their quality, safety and efficacy, including the establishment of international reference materials. (who.int)
  • The main finding was that POPs at the levels at which they are found in humans can interfere with steroidogenesis in a human adrenocortical cell line. (nih.gov)
  • The aim of the present study was to evaluate the influence of angiotensin II (Ang II) on human EC cells. (spandidos-publications.com)
  • Biological assays were performed on well-[Ishikawa (ISH)] moderately (MFE-296) and poorly (MFE-280) differentiated human adenocarcinoma cancer cell lines, representing the G1, G2 and G3 stage of EC. (spandidos-publications.com)
  • In the present work, we have isolated and characterized the extracellular vesicles from human AD-MSC to investigate their role in the chondroprotective actions of these cells. (karger.com)
  • Assay of blood cholinesterase activities is the most common and reliable biological indicator of human exposure to organophosphorous pesticides (OPs). (cdc.gov)
  • The anti‐proliferative activity of all newly synthesized compounds has been assessed against six human solid tumor cell lines. (researchgate.net)
  • Compound 5 showed high antiproliferative activity against several human tumor cell lines. (researchgate.net)
  • The two dosing modes yielded similar dose-dependent hormonal responses of H295R cells. (nih.gov)
  • Change of interferon-γ-producing T-cell responses to severe acute respiratory syndrome coronavirus 2-specific overlapping peptides in paired samples classified with symptom severities. (ajtmh.org)
  • The IL-1 alpha precursor reportedly shows full biological activity in the EL-4 assay. (rndsystems.com)
  • Concomitant with the reduction of HH pathway activity, Tctn2 mutants exhibited increased cell death in the neurectoderm and facial ectoderm, culminating in a collapse of the facial midline. (elifesciences.org)
  • Seven compounds show good activity against single cell line whereas, compounds having a pyridine scaffold showed significant anti‐proliferative activity. (researchgate.net)
  • The individual synthetic tetrapeptide substrates, together with the caspase inhibitors and active caspase enzymes, offer flexibility in the experimental design of a caspase activity assay. (bdbiosciences.com)
  • MRFR is the Leading Brand in The Research Company who Recently Published Immunofluorescence Assay Market Research Reports which includes Study of growth, Regional Analysis, Top Industry Players Formation, Major Drivers, Upcoming Trends and Forecast to 2023. (medgadget.com)
  • Taken together, one important mechanism for obese adipose CD4+ T cell activation may be mediated through MHCII expressed on ATMs and adipocytes. (exposed-skin-care.net)
  • However, its role in aging-related adipose tissue CD4+ T cell activation remains to be investigated. (exposed-skin-care.net)
  • CD8+ T Cell Activation Compared to CD4+ T cells, CD8+ T cells show a greater increase in adipose tissue in obesity and in aging (31, 43, 106). (exposed-skin-care.net)
  • Similar to CD4+ T cells, CD8+ T cells exhibit effector memory or effector phenotypes expressing elevated levels of IFN- in obese adipose tissue (31, 44). (exposed-skin-care.net)
  • The mechanism for CD8+ T cell activation in adipose tissue is not fully understood. (exposed-skin-care.net)
  • Adipose tissue-derived mesenchymal stem cells (AD-MSC) show great potential to treat inflammatory and degradative processes in OA and have demonstrated paracrine effects in chondrocytes. (karger.com)
  • After Ang II treatment, poorly differentiated endometrial cancer cell line acquired a mesenchymal phenotype, which was characterized by induced expression of EMT-related genes (VIM, CD44, SNAI1, ZEB1 and ZEB2). (spandidos-publications.com)
  • The injectable polio vaccine developed by Jonas Salk was one of the first products mass-produced using cell culture techniques. (wikipedia.org)
  • This vaccine was made possible by the cell culture research of John Franklin Enders , Thomas Huckle Weller , and Frederick Chapman Robbins , who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)
  • In particular, the biological assay of potency recommended in 1991 had been shown not to correlate with the efficacy of the vaccine in infants, nor to provide a sensitive indicator of vaccine quality. (who.int)
  • The assay started to be used in biological research in the early 1980s. (wikipedia.org)
  • Cell culture techniques were advanced significantly in the 1940s and 1950s to support research in virology . (wikipedia.org)
  • It has been proven that combining electroporation with chemotherapy potentiates the cytotoxicity of drugs when the drugs' efficacy is limited by its uptake in the cell [ 9 - 13 ]. (hindawi.com)
  • In 1907 the zoologist Ross Granville Harrison demonstrated the growth of frog embryonic cells that would give rise to nerve cells in a medium of clotted lymph . (wikipedia.org)
  • To help assess the accuracy of a particular assay, positive and negative controls should be performed. (wikipedia.org)
  • The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
  • New assays and biotechnologies, including physicochemical techniques, are also evaluated by the Expert Committee, as appropriate, through collaborative laboratory studies. (who.int)
  • Platelets are anucleate cells that are important for haemostasis, thrombosis, and atherosclerotic disease. (hindawi.com)
  • Majority of the synthesized compounds get filtered out in this step, hence its extremely important to set up the assays in such away that the outcome provide true reliable measure of effect of each compound. (brighttalk.com)
  • T helper 2 cells many important characteristics of chemical allergens are common to both contact and respiratory sensitizers. (cdc.gov)
  • Electroporation or electropermeabilization is a process where exposing cells to specific electrical pulses results in temporary formation of hydrophilic pores in the cell membrane. (hindawi.com)
  • Results from BK036-S RhoA activation assay. (cytoskeleton.com)
  • Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
  • Magnetic-based cell isolation (MACS technology) is state-of-the-art here. (ddw-online.com)