• fluorescence
  • Indeed, fluorescence microscopy has been used in high throughput screening of bacterial mutants for defects in colonization 17 , 18 , and is less laborious than other methods, including sonication 22 , 25-27 and individual nematode dissection 28 , 29 . (jove.com)
  • The general concept of the fluorescence enzyme assay is that synthetic C-, N-, or P-rich substrates bound with a fluorescent dye are added to soil samples. (jove.com)
  • bacteria
  • Building on assays described by Austin (Billaud and Austin 1990) a screening procedure has been developed in which marine bacteria are challenged by exposing them to terrestrial bacteria prior to assay of antimicrobial compounds. (gilroypheromones.com)
  • Many plasmids are available that carry genes encoding proteins that fluoresce at different wavelengths ( i.e. green or red), and conjugation of plasmids from a donor Escherichia coli strain into a recipient bacterial symbiont is successful for a broad range of bacteria. (jove.com)
  • Discriminating the frequency and load of colonizing bacteria can be especially important when screening or characterizing bacterial mutants for colonization phenotypes 21 , 24 . (jove.com)
  • species
  • In fish, up to six chromatophore types (melano-, leuco-, erythro-, xantho-, irido-, and cyanophores) have been identified, and there are two distinctive model species to which molecular genetics can be feasibly applied, the zebrafish and the medaka. (genetics.org)
  • activity
  • Experimental food pellets are presented to a generalist predator in laboratory feeding assays to assess the antipredatory activity of the extract. (jove.com)
  • types
  • Spectral absorption peaks of 500 nm and 675 nm, respectively, suggest that M. annularis and M. faveolata contain similar types of chlorophyll and chromatophores. (jove.com)
  • The chromatophores are distributed in the skin, and their types, sizes, densities, and physiological activities affect these colors and patterns. (genetics.org)
  • method
  • We describe a method for visualization of a bacterial symbiont within or on a nematode host, taking advantage of the optical transparency of nematodes when viewed by microscopy. (jove.com)
  • important
  • Results obtained using this laboratory assay are an important prelude to field experiments that rely on the feeding responses of a full complement of potential predators. (jove.com)
  • THE colors and patterns on animal body surfaces are often important for visual communication in the wild and are determined primarily by pigment cells (chromatophores) in vertebrates. (genetics.org)