• SNP detection requires high specificity and viral quantification needs both high specificity and sensitivity. (sigmaaldrich.com)
  • There are a number of factors that can be altered to obtain optimum assay performance and thereby lead to higher molecular sensitivity, specificity and precision. (sigmaaldrich.com)
  • Whenever primer dimer products are produced and amplified, they divert reaction components away from synthesis of the desired product, thereby reducing assay efficiency and sensitivity. (sigmaaldrich.com)
  • Analytical sensitivity of YFV real-time RT-PCR assays. (cdc.gov)
  • Kinetic exclusion assay have also been applied for concentration immunoassay, where it has proven capable of providing the maximum theoretical, Kd limited, sensitivity. (wikipedia.org)
  • With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL520™ based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity. (anaspec.com)
  • Sensitivity of an assay dictates the detection of the target within the dynamic range of the assay with the caveat that the lower limit of detection could vary marginally from batch to batch. (genengnews.com)
  • Technologically, luminescent and fluorescent modes offer greater sensitivity compared to absorbance assays. (genengnews.com)
  • Both assay format and detection technology play major roles in sensitivity. (genengnews.com)
  • First of all, let's not confuse the assay sensitivity with its clinical sensitivity. (medscape.com)
  • The assay sensitivity is what we do in the lab with limiting dilution of reagents, and we say, what's the least amount of molecules we can detect? (medscape.com)
  • P erhaps most significantly are the big increases in assay sensitivity and the widening of the dynamic range accomplished by using alternatives to the traditional colorimetric detection, or by the use of new platform technologies. (ddw-online.com)
  • More recently, bead-based methods and microfluidic platforms have started to impact immunoassays and by coupling with single molecule detection look set to take the sensitivity of traditional ELISA to new levels. (ddw-online.com)
  • Our experimentally verified microbial DNA qPCR assays demonstrate high specificity and sensitivity, demonstrated by the lower limit of quantification (LLOQ). (qiagen.com)
  • Then, further protocol development and standardization to achieve a clinical sensitivity for CIN2/3 detection close to what can be achieved for invasive lesions using the physician collected cervical is needed. (plos.org)
  • However, RIDTs have limited sensitivity to detect influenza virus infection and negative test results should be interpreted with caution given the potential for false negative results. (cdc.gov)
  • However, negative RIDT results do not exclude influenza virus infection as a cause of a respiratory outbreak because of the limited sensitivity of these tests. (cdc.gov)
  • More advanced diagnostic tests such as enzyme linked immunosorbent assays (ELISAs) do possess this capability but have similar drawbacks to microscopy, requiring expensive equipment, long incubation times, and trained staff. (hindawi.com)
  • Enzyme-linked immunosorbent assays (ELISA) have been around as one of the primary methods of analyte detection for more than four decades. (ddw-online.com)
  • Claims relating to the performance of assays should be verified under the conditions of the study, including test samples, specific reagents and the instrument of choice. (sigmaaldrich.com)
  • However, all assays have a set of optimal conditions and these are dependent on the instrument, selected reagents (buffer conditions), primer concentration and/or annealing temperature (T a ), magnesium concentration and even ramp rate. (sigmaaldrich.com)
  • Other automated instrumentation is designed to support the creation/coating of ELISA assay plates or contains cartridge-based delivery of assay reagents to reduce set-up time. (ddw-online.com)
  • These are supported by a wide range of ELISA reagents, assay and kits providing relative profiling or absolute quantitation of proteins across a wide range of study areas. (ddw-online.com)
  • Increasing adoption of kits & reagents and lateral flow assay readers for infectious diseases testing, veterinary diagnostics , and drug of abuse testing, cardiac marker testing . (marketsandmarkets.com)
  • Secondly, identify which diagnostic assays are being used to detect SARS CoV-2 and which VOC PCR Screening reagents you have. (who.int)
  • Sequencing of canine Melan- A and gp100 PCR products confirmed the specificity of the assay for these genes. (avma.org)
  • Plasmodium aldolase is a malaria biomarker measured using enzyme linked immunosorbent assay (ELISA) techniques. (hindawi.com)
  • The laboratory method is an Enzyme-Linked Immunosorbant Assay (ELISA). (cdc.gov)
  • This ELISA method is for the semi-quantitative detection of IgA antibodies to tissue transglutaminase (endomysium) in human serum. (cdc.gov)
  • Over the years many changes to the basic format have resulted in assay improvements, but some of the most recent look set to take the traditional ELISA to new levels. (ddw-online.com)
  • These include many vendors now addressing the automation of ELISA assays. (ddw-online.com)
  • When coupled with the breadth of existing global expertise in immunoassays, it is easy to see why ELISA still remains the methodology of choice by those labs tasked with analyte detection. (ddw-online.com)
  • E nzyme-linked immunosorbent assays (ELISA) have been one of the primary methods of detecting the presence of a substance, usually an antigen, in an aqueous sample for more than 40 years. (ddw-online.com)
  • However, numerous variants of this format have been developed over the years (eg indirect, sandwich, competitive, etc) and a multitude of detection chemistries have been applied to ELISA, including chemiluminescence and various fluorescent readouts. (ddw-online.com)
  • ELISA has gained widespread acceptance across a very diverse range of fields (eg diagnosis of infectious diseases, food allergen detection, plant pathogens to biomarkers), but it never achieved significant adoption by early drug discovery labs. (ddw-online.com)
  • The immunoassay landscape has, however, dramatically changed in recent years with the development of reduced assay steps protocols, no wash ELISAs and new automated systems designed to run ELISA. (ddw-online.com)
  • This review article uses as its starting point some of the findings of HTStec's ELISA Assay Trends 2012 survey and report, published in June 2012 (1). (ddw-online.com)
  • This report discusses end-users' current practices and preferences in ELISA assays, and seeks to understand future user requirements. (ddw-online.com)
  • The main (primary) application area for survey respondents' ELISA assays was immunology (17% selecting). (ddw-online.com)
  • These breakthrough results indicate that sHLA-G ELISA can be a useful biochemical assay in addition to embryo morphology in embryo selection for transfer in IVF treatment if there are other embryos with the same morphology. (biovendor.com)
  • Similarly, when multiple targets are to be detected simultaneously in multiplex reactions, assay conditions must be optimized to detect all targets equally. (sigmaaldrich.com)
  • Objective -To develop a reverse transcriptase-polymerase chain reaction (RT-PCR) assay to detect canine melanoma-associated antigens (MAAs) and to use this technique to screen aspirates of lymph nodes (LNs) for evidence of metastatic spread of oral malignant melanoma. (avma.org)
  • In the present study, the limiting dilution assay (LDA) and the ELISpot are compared in their ability to detect serogroup C meningococcal (MenC)-specific memory B-cells in peripheral blood of 12month old children, after 3-dose priming with serogroup C meningococcal conjugate vaccine (MenCV) in infancy. (ox.ac.uk)
  • What remains unclear, and is regularly (and at times heatedly) put to debate , is whether less sensitive toxin assays, which detect the actual C. difficile toxin proteins (A and B) that cause CDI, are effective at winnowing out carriage cases and identifying CDI when it really needs to be treated. (asm.org)
  • Toxin assay fans could find solace in the exceptionally low limit of detection for toxin, as well as the possibility that perhaps the CDI-NAAT group was too inclusive of patients with low concentrations of toxin that didn't have as many (or any) bad CDI-related outcomes, and thus, were less important to detect. (asm.org)
  • The assays enable you to detect microbial species, virulence genes or antibiotic resistance genes using a simple qPCR workflow. (qiagen.com)
  • This study demonstrated that use of a PCR assay to detect spores in rain samples collected at geographically dispersed NADP sites can serve as a powerful early warning system for the movement of P. graminis. (usda.gov)
  • Quantitative assays for HCV RNA Tests to detect HCV RNA concentration (viral load) by amplification of viral genetic sequences or by signal amplification. (cdc.gov)
  • However, standardization of eDNA methods and reporting across the field is yet to be fully established, with one area being the calculation and interpretation of assay limit of detection (LOD) and limit of quantification (LOQ). (usgs.gov)
  • This study reports the development of a sensitive magnetic bead-based enzyme-linked immunoassay (MELISA) for the pan-reactive detection of the Influenza A virus. (flutrackers.com)
  • We utilize datasets from multiple cooperating laboratories to demonstrate both a discrete threshold approach and a curve‐fitting modeling approach for determining LODs and LOQs for eDNA qPCR assays. (usgs.gov)
  • Ultimately, standardization of how LOD and LOQ are determined, interpreted, and reported for eDNA assays will allow for more informed interpretation of assay results, more meaningful interlaboratory comparisons of experiments, and enhanced capacity for assessing the relative technical quality and performance of different eDNA qPCR assays. (usgs.gov)
  • The recombinant immunoblot assay is used to confirm HCV infection. (medscape.com)
  • RIBATM Recombinant immunoblot assay. (cdc.gov)
  • EscapePlex or L452R single mutation assay) that has the capacity to identify Delta variant. (who.int)
  • Molecular staging of melanomas in dogs can be achieved by screening LN aspirates for MAA mRNA, and this can be performed in combination with cytologic examination to aid in detection of metastatic disease. (avma.org)
  • 1 As a matter of the fact, the competence needed to validate molecular diagnostic assays is beyond most cytopathology laboratories and, even if there was expertise, a larger number of cases is required to run in-house testing cost-effectively. (bmj.com)
  • RT 2 qPCR Primer Assays are intended for molecular biology applications. (qiagen.com)
  • 1 RIDTs do not include rapid molecular assays. (cdc.gov)
  • Rapid molecular assays are a new type of influenza diagnostic test that use isothermal nucleic acid amplification for viral detection. (cdc.gov)
  • At present, only one rapid molecular assay is FDA-approved for use in the United States. (cdc.gov)
  • Comparison of a limiting dilution assay and ELISpot for detection of memory B-cells before and after immunisation with a protein-polysaccharide conjugate vaccine in children. (ox.ac.uk)
  • Methods The limit of detection (LOD) of the Idylla EGFR prototype assay was assessed by cell line dilution studies. (bmj.com)
  • This Catalyze Product Definition Funding Opportunity Announcement (FOA) will provide the early stage translational support needed to develop and test device prototype designs, identify diagnostic disease targets and develop associated assays, and develop research tools for use in the treatment of heart, lung, blood and sleep (HLBS) diseases and disorders. (nih.gov)
  • The Genie Fast HIV1/2 Assay Kit is a high-performing diagnostic tool that leverages Lateral Flow technology for swift and accurate identification of HIV-1 and HIV-2 antibodies in human samples. (procure-net.com)
  • The Genie Fast HIV1/2 Assay Kit is a state-of-the-art diagnostic tool specially designed for swift detection of HIV-1 and HIV-2 antibodies present in human plasma, serum, or whole blood specimens. (procure-net.com)
  • An important question in the field of infectious disease diagnostic testing is whether or not Clostridium difficile ( C. difficile ) toxin protein assays (hereafter, toxin assays) differentiate C. difficile infection (CDI) from mere carriage of toxigenic C. difficile in the GI tract. (asm.org)
  • Conclusions Idylla EGFR prototype assay is sensitive on extracted DNA and can reliably be applied on cytological samples, enabling implementation of EGFR testing even in less experienced diagnostic units. (bmj.com)
  • As with the identification of the Alpha variant in the UK, the lab team in South Africa detected an increasing number of specimens with an S gene target dropout using the Thermo Fisher TaqPath COVID-19 Combo Kit as their diagnostic assay. (who.int)
  • If you are using the Thermo Fisher TaqPath COVID-19 Combo Kit (3 target PCR assay) as your diagnostic reagent, consider the results below as an indication of a sample which might be Omicron variant. (who.int)
  • A membrane receptor reagent and assay is disclosed in which liposomes are bound to an evanescent wave emitting surface. (google.com)
  • The global lateral flow assays market in terms of revenue was estimated to be worth $20.5 billion in 2022 and is poised to reach $22.6 billion by 2027, growing at a CAGR of 2.0% from 2022 to 2027. (marketsandmarkets.com)
  • Whereas most of lateral flow assays are having limitation of qualitative detection, lot to lot variation like factors may hinder the growth of global market during the forecast period. (marketsandmarkets.com)
  • This would impact health expenditure and thus negatively impact all healthcare product and service markets, including the lateral flow assay market. (marketsandmarkets.com)
  • The advantages of lateral flow assay tests in detecting COVID-19 antibodies include their affordability, ease of interpretation, and speedy diagnosis (within 15 minutes). (marketsandmarkets.com)
  • Since these tests can also be produced economically, all the major players in the lateral flow assay market launched COVID-19 tests in the past year. (marketsandmarkets.com)
  • Although easy to perform and more economical than laboratory tests, lateral flow assay tests have been observed to have lower accuracy. (marketsandmarkets.com)
  • The factors that need to be considered include: selection of the most appropriate assay technologies, reading modalities, and experimental models (biochemical, cellular, or animal). (genengnews.com)
  • Biochemical assays are simpler to execute than their cellular counterparts and offer better consistency. (genengnews.com)
  • Hence, it is desirable to start with a biochemical assay followed by a cell-based assay to corroborate results in a physiological context (that is, to provide orthogonal validation). (genengnews.com)
  • The colorimetric detection signal was amplified using biotinylated silica nanoparticles (NP). (flutrackers.com)
  • A biotin-nanoparticle and immunomagnetic bead-based assay was developed. (flutrackers.com)
  • This paper describes the design, fabrication, and testing of a pneumatically controlled, renewable, microfluidic device for conducting bead-based assays in an automated sequential injection analysis system. (cdc.gov)
  • RT² qPCR Primer Assays are specifically designed and experimentally verified for real-time PCR analysis. (qiagen.com)
  • Each assay utilizes a proprietary and experimentally verified algorithm for the design of gene-specific qPCR primers with uniform PCR efficiency and amplification conditions. (qiagen.com)
  • Kinetic exclusion assays have been performed using unpurified molecules, in serum, and have measured binding to cell membrane proteins on intact whole cell which brings the measured binding interactions closer to their endogenous state. (wikipedia.org)
  • Assay: Phenylalanine in human serum. (bioassaysys.com)
  • Serum levels of L-FABP were determined by enzyme-linked immunosorbent assay. (scirp.org)
  • 4] Detection of anti-HBs in the serum implies either active or passive immunization that usually persists for life. (medscape.com)
  • Since its initial detection Omicron has been identified in countries in four of the six WHO Regions. (who.int)
  • The relative prevalence of each mutation in Omicron sequences in not yet known as the sequencing data at the present time is limited. (who.int)
  • This technical note aims to provide information to member states for using PCR Assays as a screening tool for Omicron variant. (who.int)
  • Screening for the suggested presence of Omicron VOC can be performed by identifying S gene target failure (SGTF) or by using assays that target specific mutations. (who.int)
  • In contrast, a 2019 guideline on colorectal cancer screening from an international panel of experts recommends using risk calculations to guide screening, with screening limited to patients with an elevated level of risk. (medscape.com)
  • Optimization is required to ensure that the assay is as sensitive as is required and that it is specific to the target of interest. (sigmaaldrich.com)
  • Although these RDTs are cheap, fast, and easy-to-use, they do not provide a quantitative estimate of parasitic burden and are less sensitive for detection of Plasmodium vivax [ 1 , 2 ]. (hindawi.com)
  • An example of this technique has been employed for sensitive detection of environmental contaminants in near real-time. (wikipedia.org)
  • Plot of C. diff Toxin A concentrations detected using ultra sensitive Simoa assay, in NAAT positive patients, with and without C. diff infection. (asm.org)
  • Although there is a correlation between CDI and very high toxin concentrations, results for the 2 groups of patients are extensively overlapping, and one can see how many patients designated as CDI would be missed by the less sensitive assay. (asm.org)
  • RT² qPCR Primer Assays use SYBR ® Green-based quantitative real-time PCR technology to provide a sensitive and reliable tool for gene expression analysis. (qiagen.com)
  • Capillary zone electrophoresis was employed for the determination of metronidazole using end-column amperometric detection with a gold microelectrode at a constant potential of -0.52V vs. saturated calomel electrode. (unboundmedicine.com)
  • AU - Jin,W, AU - Li,W, AU - Xu,Q, AU - Dong,Q, PY - 2000/5/29/pubmed PY - 2000/9/23/medline PY - 2000/5/29/entrez SP - 1409 EP - 14 JF - Electrophoresis JO - Electrophoresis VL - 21 IS - 7 N2 - Capillary zone electrophoresis was employed for the determination of metronidazole using end-column amperometric detection with a gold microelectrode at a constant potential of -0.52V vs. saturated calomel electrode. (unboundmedicine.com)
  • Tissue transglutaminase assay (IgA-TTG) and the IgA endomysial antibody assay (IgA EMA) estimates the prevalence of celiac disease in the United States population. (cdc.gov)
  • We have devised a simple immunofluorometric sandwich assay for measurements of the concentrations of APC-PCI complex, employing as the catcher, a monoclonal antibody that has a high affinity (K(D) = 4 x 10(-11) M) for a complexation-specific neoepitope that is expressed on PCI. (lu.se)
  • 7] Detection of HBe antibody can also be used to monitor response to treatment and possible remission of the disease. (medscape.com)
  • This study assessed whether an Idylla EGFR prototype assay can be reliably applied to cytological lung cancer specimens. (bmj.com)
  • Assay optimization and validation are essential, even when using assays that have been predesigned and commercially obtained. (sigmaaldrich.com)
  • Assays requiring high specificity are particularly vulnerable when performed without optimization and adequate controls. (sigmaaldrich.com)
  • Since it is usual to run the assay on a selected instrument and under standard buffer conditions, most optimization procedures are focussed on modification of primer binding kinetics using primer concentration or annealing temperature (T a )/melting temperature (T m ). (sigmaaldrich.com)
  • Optimization of experimental assay design is crucial for the overall success of any drug discovery initiative. (genengnews.com)
  • The assay combines immunomagnetic beads and biotin-nanoparticle-based detection to quantify a highly conserved viral nucleoprotein in virus lysates. (flutrackers.com)
  • The assay utilizes primers specific for rust fungi and a P. graminis specific TaqMan probe nested within a general primer pair within the ITS1 region of the rDNA. (usda.gov)
  • BioAssay Systems' L-Phenylalanine Assay Kit provides a convenient fluorimetric means to measure L-phenylalanine in biological samples. (bioassaysys.com)
  • When combined with karyotyping, this assay provides increased interrogation of specific chromosomal regions, while limiting VOUS identification. (nih.gov)
  • Data describe a designed environmental DNA (eDNA) survey for the detection of oyster mussel (Epioblasma capsaeformis) from field collected water samples. (usgs.gov)
  • However, data from 2 recently published papers, both of which utilize a new ultrasensitive assay (Simoa) for detecting C. difficile toxins A and B with sub-picogram limits of detection, strongly suggest the opposite. (asm.org)
  • Validation of primer design is particularly important when adopting primers from a previous publication or using a commercially supplied assay. (sigmaaldrich.com)
  • With dye-based detection such as SYBR Green I, primer dimers also affect assay specificity because the nonspecific DNA-binding dye will bind to the primers and be detected along with the desired product. (sigmaaldrich.com)
  • The RTPCR assay was performed by use of tyrosinase, Melan-A, gp100, tyrosinase-related protein 2 (TRP-2), or melanoma antigen-encoding gene B (MAGE-B)- specific primers. (avma.org)
  • Idylla results were compared with fragment length (exon 19 del) and Taqman assays. (bmj.com)
  • The rigorous assay verification criteria ensure PCR specificity and efficiency for reliable and accurate gene expression analysis results. (qiagen.com)
  • This study reports the urinary detection of E6 oncoprotein in vivo for the first time and our results suggest that this detection is only for invasive/microinvasive lesions. (plos.org)
  • However, due to th e limited sensitivities and predictive values of RIDTs, negative results of RIDTs do not exclude influenza virus infection in patients with signs and symptoms suggestive of influenza. (cdc.gov)
  • Phenotypic cellular assay models more accurately reflect the complex biology of disease, allows for multiparametric measurement of ex vivo or in vivo events, and enables the characterization of intractable target(s). (genengnews.com)
  • During the assay, a fraction of the free receptor is captured by the solid phase ligand and subsequently labeled with a fluorescent secondary molecule (Figure 1). (wikipedia.org)
  • Because the fluorescent secondary molecule is applied after capture of the free receptor from solution (Figure 2) the binding constants measured using a kinetic exclusion assay are for unmodified molecules in solution and thus more accurately reflects endogenous binding interactions than methods requiring modification (typically labeling or immobilization) before measurement. (wikipedia.org)
  • In the assay, L-phenylalanine is oxidized by phenylalanine dehydrogenase, producing NADH, which reduces a fluorescent dye to a highly fluorescent product. (bioassaysys.com)
  • The assay was linear over the concentration range 0.50-500.00 ng/mL, with correlation coefficients ≥0.995. (unboundmedicine.com)
  • These assays are 97% specific but cannot distinguish acute from chronic infection. (medscape.com)
  • characterized by detection of HCV RNA greater than or equal to 6 months after newly acquired infection. (cdc.gov)
  • Detection of IgG anti-HBc indicates previous or ongoing infection. (medscape.com)
  • Furthermore, measurable binding affinities for various analyte interactions can vary based on the chemistry and detection technology ( Figure 1B ). (genengnews.com)
  • more than 1000 clinically relevant antibiotic resistance genes, a few hundred phylogenetic marker genes and virulence factors have to be targeted (including SNP detection). (europa.eu)
  • Multiple RT 2 qPCR Primer Assays can also be used to examine a focused panel of genes. (qiagen.com)
  • A kinetic exclusion assay (KinExA) is a type of bioassay in which a solution containing receptor, ligand, and receptor-ligand complex is briefly exposed to additional ligand immobilized on a solid phase. (wikipedia.org)
  • The World Health Organization (WHO) recommends nucleic acid testing for qualitative or quantitative HCV RNA detection as well as for test of cure at 12 or 24 weeks following antiviral treatment completion. (medscape.com)
  • Therefore, primer dimers are an issue in reactions using both probe-based and SYBR Green I dye-based detection. (sigmaaldrich.com)
  • Detection of the analytes was achieved using positive ion electrospray ionization via multiple reactions monitoring mode. (unboundmedicine.com)
  • LOD: lower limit of detection. (cdc.gov)
  • The dynamic range of an assay defines both the lower and upper limits of detection. (genengnews.com)
  • The added detection above karyotype was 1 in 745 in lower-risk cases with normal ultrasounds or isolated ultrasound markers/increased nuchal measurements and 1 in 165 for fetuses with structural/growth abnormalities. (nih.gov)
  • The lower limit of quantitation was set at 0.5 ng/mL plasma based on an average signal-to-noise ratio of 44.79. (unboundmedicine.com)
  • Lower limits of the assay are roughly 1-10 spores per sample based on spiking experiments. (usda.gov)
  • Precious" samples, such as limited cells or tissue, impact the permissible volume for testing. (genengnews.com)
  • We tested 2,970 prenatal samples of all referral indications using a rapid BACs-on-Beads-based assay with probes for sex chromosomes, common autosomal aneuploidies, and 20 microdeletion/microduplication syndromes, designed as an alternative to microarray in low-risk pregnancies and an alternative to rapid aneuploidy testing in pregnancies also undergoing microarray analysis. (nih.gov)
  • If assays are not completed within 48 hours, or the separated sample is to be stored beyond 48 hours, samples should be frozen at -20 degrees Celsius or colder. (medscape.com)
  • Upon completion of the assay process, the pressure is released and the beads are flushed out from underneath the filter structure to renew the device. (cdc.gov)
  • The method gives a linear dose-response curve (0.06-50 microg/l), has a low detection limit (0.06 microg/l) and no crossreactivity with native PCI at physiologic plasma concentrations. (lu.se)
  • Wash-based assays can be used to circumvent interference caused by highly complex sample types, as molecules within the matrix that contribute to interference are washed away. (genengnews.com)
  • The Idylla EGFR prototype assay is a rapid and fully automated test which may easily be adopted by a wider number of pathological centres. (bmj.com)
  • A total of 10 ng was directly placed inside an Idylla EGFR prototype assay cartridge. (bmj.com)
  • Each lot of every assay is further wet-bench tested for real-time PCR performance for specificity and amplification efficiency. (qiagen.com)
  • Defining the paradigms and predictable end point(s) enables selection of the assay formats and technologies to be used for the study. (genengnews.com)
  • The authors of the first Simoa paper came back with another beautiful study that could be interpreted as closing the case of whether toxin assays can be used to differentiate CDI from carriage. (asm.org)
  • The study aims to compare the detection rates of high-risk human papillomavirus 16 and 18 E6 oncoprotein in urine, vaginal self-collected, and cervical scrapes of women using the Onco E6 ™ Cervical Test and compare the HPV16 and/or HPV18 E6 detection rates with the HPV DNA testing. (plos.org)
  • The optimum conditions of separation and detection are 1.0 x 10(-3) mol/L potassium dihydrogen citrate (KH2C6H5O7) for the buffer solution, 20 kV for the separation voltage, and 5 kV and 10 S for injection voltage and injection time, respectively. (unboundmedicine.com)
  • The Comet Assay (also called Single Cell Gel Electrophoresis) is used to assess DNA damage and repair in individual cells. (neb.com)