• This report analyzes the cancer profiling and pathways market: technologies market, tools market, and application market (diagnosis, drug development and discovery). (bccresearch.com)
  • Nanosilicates dissociate into individual minerals inside the cells and turn "on" a set of key genes that result in information flow throughout the cells, known as signaling pathways. (sciencedaily.com)
  • Combining interdisciplinary techniques and biomedical engineering and genomics methods, the lead authors of this study, doctoral students Anna Brokesh and Lauren Cross, identify and characterize significant genes that are turned "on" and activated by different signaling pathways due to treatment with minerals. (sciencedaily.com)
  • RNA-seq, a transcriptome-wide high throughput sequencing assay, provides an unbiased and holistic overview of the gene expression profiles to identify pathways that are perturbed by specific treatments. (sciencedaily.com)
  • What pathways are this gene/protein implicaed in? (cancerindex.org)
  • These genes encode proteins involved in DNA replication and cell cycle/proliferation that contribute to major pathways responsible for suppression of colorectal carcinogenesis pathways. (iiarjournals.org)
  • Conclusion: SCFAs inhibited the expression of genes encoding proteins involved in DNA replication and cell cycle/proliferation of human colorectal cancer cells and exerted antiproliferative activity via different pathways. (iiarjournals.org)
  • Currently, we need systems that will allow us to progress from gene discovery to the illumination of relevant biological pathways and novel therapeutics. (sfari.org)
  • Results of these comparisons will elucidate biological pathways that are likely to be disrupted by the ASD risk genes, and will identify compounds with the potential to selectively suppress specific behavioral phenotypes arising from these mutations. (sfari.org)
  • Finally, by searching for enrichment of specific neurochemical pathways in the pharmacological and gene expression studies, the team aims to identify common biological mechanisms and promising pharmacological candidates for the treatment of ASD. (sfari.org)
  • EN2 expression leads to the activation of multiple signaling pathways mediated by phosphorylation cascades. (bvsalud.org)
  • The goal of our search was to identify publications describing the role of specific genes, gene signatures, or molecular pathways and their effects in response to environmental exposures. (cdc.gov)
  • In recent years, there has been an effort toward developing genomic assays as a predictive and prognostic tool to improve precision in estimating disease recurrence, sensitivity to systemic treatment and ultimately with clinical utility for guidance regarding adjuvant systemic treatment(s). (nih.gov)
  • Many diagnostic and prognostic assays have begun to show clinical applicability providing additional tools to optimize and individualize treatments. (biomedcentral.com)
  • Second, a focused gene expression assay including prognostic genes was used to develop a robust clinical assay (GCPS) in stage II patients from the same cohort (N=186). (duke.edu)
  • Furthermore, we found homogenous and recurrent iCNV profiles in the high-grade tumors of relapse patients and identified expression of gene SPP1 in the tumor stroma as a potential prognostic mRNA marker in pediatric brain tumor relapse patients. (kth.se)
  • With a variety of assays we could show that MSC represent a cell population which can be expanded for therapeutic applications. (biomedcentral.com)
  • Microarray technologies provide analysis of tens of thousands of molecules for a variety of assays, including drug binding, molecular interactions, enzyme activity and pathway identification. (bccresearch.com)
  • We analyzed 137 B-lineage and 30 T-lineage childhood ALL cases using microarray analysis of DNA copy number alterations and gene expression, and genome-wide cytosine methylation profiling using the HpaII tiny fragment enrichment by ligation-mediated PCR (HELP) assay. (lu.se)
  • We found that the different genetic subtypes of ALL are characterized by distinct DNA methylation signatures that exhibit significant correlation with gene expression profiles. (lu.se)
  • Finally, we identified aberrant methylation in multiple genes also targeted by recurring DNA copy number alterations in ALL, suggesting that these genes are inactivated far more frequently than suggested by structural genomic analyses alone. (lu.se)
  • Sarcoma tissue samples and cell lines are subjected to a multiplatform genome-wide characterization of expression of protein-coding genes and microRNAs, DNA copy number changes, activating mutations, gene rearrangements and methylation profiling. (mskcc.org)
  • We aimed to investigate whether the molecular subtypes also display distinct methylation profiles. (lu.se)
  • Methods: We analysed methylation status of 807 cancer-related genes in 189 fresh frozen primary breast tumours and four normal breast tissue samples using an array-based methylation assay. (lu.se)
  • Finally, by utilizing gene expression data, we observed that a large fraction of genes reported as having subtype-specific expression patterns might be regulated through methylation. (lu.se)
  • Conclusions: We have found that breast cancers of the basal-like, luminal A and luminal B molecular subtypes harbour specific methylation profiles. (lu.se)
  • In Paper III , we present a protocol for performing spatial mRNA genome-wide expression profiling of FFPE tissue specimens. (kth.se)
  • Looking for an experienced Postdoctoral Fellow to join an NIH funded laboratory dedicated to gene target identification and drug discovery in soft tissue sarcoma through a genome-wide genetic and functional analysis. (mskcc.org)
  • More than half (23 of 39) of the articles represent epidemiologic studies including candidate gene and genome-wide association studies (GWAS) examining genetic effect modification on disease risk. (cdc.gov)
  • Intracellular amastigote protocols need further refinement, but we describe a first generation tool for high-throughput comparative molecular characterization of patients' isolates, based on the changing expression profiles of promastigotes during in vitro differentiation. (cambridge.org)
  • In addition, their osteogenic differentiation potential was increased, and genes involved in cell adhesion, FGF-2 signalling, cell cycle, stemness, cell differentiation, and cell proliferation were upregulated, compared to that of the MSCs cultured on uncoated plates. (hindawi.com)
  • To address this question we first developed human oligonucleotide microarrays with 30.000 elements and then performed large-scale expression profiling of long-term expanded MSC and MSC during differentiation into osteoblasts. (biomedcentral.com)
  • The results showed that MSC did not alter their osteogenic differentiation capacity, surface marker profile, and the expression profiles of MSC during expansion. (biomedcentral.com)
  • Microarray analysis of MSC during osteogenic differentiation identified three candidate genes for further examination and functional analysis: ID4, CRYAB, and SORT1. (biomedcentral.com)
  • We monitored these cells during their expansion ex vivo with respect to proliferation kinetics, surface marker profile and differentiation potential. (biomedcentral.com)
  • Finally we analyzed the gene expression profiles of MSC during osteogenic differentiation. (biomedcentral.com)
  • The effects of PTE on osteoclast differentiation and the expression of osteoclast-specific genes and proteins were determined. (researchgate.net)
  • These nanoparticles are known specifically as nanosilicates, and with them, the team is able to determine the role of minerals in regulating gene expression profiles to direct stem cell differentiation. (sciencedaily.com)
  • To identify potential therapeutic targets, our lab screens the genes and microRNAs in these signatures for involvement in proliferation, differentiation, and survival of sarcoma cell lines. (mskcc.org)
  • Osteogenic differentiation and bone formation assays in culture and in vivo were employed to gauge for origin in bone marrow-derived mesenchymal stem cells (bMSCs). (lu.se)
  • These microarrays, which include DNA microarrays, protein microarrays, tissue microarrays, low complexity microarrays and carbohydrate microarrays, are excellent tools for gene expression profiling, biomarker profiling and diagnostics. (bccresearch.com)
  • Expression profiling was carried out on samples of 7 benign, 7 LMP and 28 invasive (moderate and poorly differentiated) serous tumors and four whole normal ovaries using oligonucleotide microarrays representing over 21,000 genes. (biomedcentral.com)
  • We will also focus on these genomic assays' clinical application and utility in node-positive early-stage BC based on the most recent evidence and guidance recommendations. (nih.gov)
  • The purpose of this study was to develop a robust, standardized method for comparative expression profiling and molecular characterization of Leishmania donovani clinical isolates. (cambridge.org)
  • Follow-up integration of daSNV gene editing with clinical cohort analyses suggested that magnesium transport dysfunction may increase neuropsychiatric disease risk and indicated that common genetic pathomechanisms may mediate specific symptoms that are shared across multiple neuropsychiatric diseases. (stanford.edu)
  • HCT in EAE mice leads to an enhancement of this myeloid state, as well as clinical improvement, reduction of demyelinated lesions, suppression of cytotoxic T cells, and amelioration of reactive astrogliosis reflected in reduced expression of EAE-associated gene signatures in oligodendrocytes and astrocytes. (stanford.edu)
  • The cells' gene expression signatures were tested for association with clinical outcome of breast cancer patients using survival data from The Cancer Genome Atlas database. (lu.se)
  • Two leading clinicians debated whether the differences between the three groups have clinical relevance or whether the degree of HER2 expression is merely a marker for other disease processes. (medscape.com)
  • The Singh Laboratory, managed by Singh, leverages high-throughput functional assays and perturbations to dissect the functional regulatory programs in mammalian cells. (sciencedaily.com)
  • 10x Genomics has launched its Feature Barcode Multiplexing Kit to expand the capabilities of its Chromium Single-Cell Gene Expression Flex assay to include high-throughput multiomic cellular profiling. (genomeweb.com)
  • Using a high-throughput in vivo approach, Hoffman and her team previously identified dysfunction of excitatory and inhibitory systems in zebrafish lacking a functional autism risk gene, CNTNAP2 . (sfari.org)
  • High-throughput RNA sequencing (RNA-seq) has revolutionized our understanding of gene expression. (cdc.gov)
  • Protein crystallization is a key assay for structural studies of proteins. (gracebio.com)
  • Additionally, PTE efficaciously suppressed the expression of key osteoclast-specific genes and proteins. (researchgate.net)
  • A collaboration between researchers in the Department of Biomedical Engineering and the College of Medicine at Texas A&M University identifies the crucial role of minerals in regulating gene expression, thus controlling the number of proteins that a cell should make, thereby encouraging tissue regeneration and redefining cellular identity. (sciencedaily.com)
  • In eukaryotic cells, chromatin comprises a complex consisting of DNA, RNA, and proteins where covalent modifications correlate with regulation of gene expression [ 1 ]. (biomedcentral.com)
  • 135 antibodies against 65 mainly immunoregulatory proteins, we of a multitude of different genes (8, 9). (lu.se)
  • Like Rb protein, many of the proteins encoded by tumor suppressor genes act at specific points in the cell cycle. (medscape.com)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • Hoffman's team aims to extend this work by investigating zebrafish with mutations in nine strong ASD candidate genes, previously identified from whole-exome sequencing of the Simons Simplex Collection (SSC) . (sfari.org)
  • Behavioral profiles arising after exposure to psychoactive compounds will be compared in zebrafish containing mutations in ASD genes and in wildtype zebrafish. (sfari.org)
  • Accumulating evidence shows that tRNA expression and mutations are associated with various diseases such as neurological pathologies and cancer development3,4. (cdc.gov)
  • This paper will review the Oncotype DX 21-gene Recurrence Score (RS), MammaPrint, EndoPredict, Prosigna ® , and Breast Cancer Index (BCI) genomic assays. (nih.gov)
  • As reviewed in this issue of The ASCO Post , Losk and colleagues from Dana-Farber/Brigham and Women's Cancer Center report their institution's implementation of a protocol of surgeon-initiated genomic profiling of estrogen receptor-positive tumors in. (ascopost.com)
  • In fact, UMIs (unique molecular indices) entirely remove PCR bias in RNAseq counting (see figure Unmatched uniformity (>97% of assays are within 20% of median molecular tag counts) A 917-plex gene panel was used to prepare a library from 10 ng of NA12878 reference DNA. All assays were designed to be intra-exon, and are thus single copy on genomic DNA. This allows an estimation of the uniformity of amplicon performance in the library preparation step (e.g., every unique tag equals one captured copy). In terms of raw assay performance, 97.5% of assays are within 20% of mean/median molecular tag counts. For cataloged panels, any assay below 20% is redesigned and replaced. Molecular barcodes entirely remove this variation in RNAseq counting. "> Unmatched uniformity - 97% of assays are within 20% of median molecular tag counts ). (qiagen.com)
  • Quantitative real-time PCR was used to test the expression of ZmCAMTA genes under several abiotic stresses (drought, salt, and cold), various stress-related hormones [abscisic acid, auxin, salicylic acid (SA), and jasmonic acid] and biotic stress [rice black-streaked dwarf virus (RBSDV) infection]. (frontiersin.org)
  • We investigated the expression of genes of interest using quantitative reverse transcription PCR. (molvis.org)
  • Quantitative real-time PCR (qRT-PCR) is the gold standard in any gene expression experimentation and is also available for the detection of miRNAs at any stage of maturation. (bitesizebio.com)
  • QIAseq Targeted RNA Panels have been developed as a Sample to Insight solution for quantitative gene expression profiling using RNAseq. (qiagen.com)
  • Here, we used quantitative real-time PCR, profiling the expression of 93 genes in single-cells from three different cell lines. (frontiersin.org)
  • Supplementary Table 11: Details of variations in cis elements between IR64 and N22 genotypes for 20 N transporter, N regulator, TOND1, Dof genes and candidate genes identified from the major QTLs mapped in the present study. (figshare.com)
  • Supplementary Table 12: miRNAs in the candidate genes present in interval of the QTL hotspot region identified on chromosome 6. (figshare.com)
  • MicroRNA miR-29c regulates RAG1 expression and modulates V(D)J recombination during B cell development. (harvard.edu)
  • This report also examines recent studies, microRNA detection and profiling, clinically oriented microRNA profiling in several human cancers. (bccresearch.com)
  • This included sessions dedicated to systems biology on immunotherapy, immunogenicity and gene expression profiling, biomarkers, and combination treatment strategies. (biomedcentral.com)
  • The report categorizes the biomarkers and profiling market and provides market data, market drivers, trends and opportunities, top-selling products, key players and competitive outlook. (bccresearch.com)
  • Five genes that were differentially expressed between invasive and either benign or normal tissues were validated by real time PCR in an independent panel of 46 serous tumors (4 benign, 7 LMP, 35 invasive). (biomedcentral.com)
  • To identify genes associated with neoplastic progression in the serous subtype of ovarian tumors, we compared gene expression in tissues that exhibited the spectrum of tumor behavior, namely benign, low malignant potential (LMP) and invasive. (biomedcentral.com)
  • In order to clarify the molecular relationships among the spectrum of serous ovarian tumors, we compared gene expression profiles of 7 LMP and 28 invasive (moderate and poorly differentiated or Grade 2 and Grade 3) serous ovarian tumors with two different reference groups: 7 serous benign ovarian tumors and four normal whole ovary specimens. (biomedcentral.com)
  • These initial observations were confirmed in human patient samples, as cholesterol content in CD8 + T cells isolated from primary colon tumors or myeloma samples correlated with increased PD-1 and 2B4 expression. (aacrjournals.org)
  • SAN ANTONIO - Should breast cancer patients with low but not negative tumor expression of HER2 be considered a distinct patient group that can be treated differently from patients with tumors that are HER2 negative or HER2 positive? (medscape.com)
  • Although carcinogenic roles for the INK4B, INK4C, INK4D, CIP1, KIP1, and KIP2 genes appear to be limited, INK4A is among the most commonly mutated genes in human tumors. (medscape.com)
  • Recent technological advances have enabled massively parallel chromatin profiling with scATAC-seq (single-cell assay for transposase accessible chromatin by sequencing). (nature.com)
  • Integrating epigenomic and transcriptomic data with massively parallel reporter assays identified differentially-active single-nucleotide variants (daSNVs) in specific neural cell types. (stanford.edu)
  • INDIGO's assay kit for the Expression Profiling of Clinically Relevant CYPs contains optimized reagents for the culturing and treatment of upcyte ® hepatocytes to assess drug-induced changes in seven clinically-relevant CYPs: CYP3A4. (indigobiosciences.com)
  • Additional file 1: Supplementary Table 1: All differentially expressed genes identified under N-W+, N + W- and N_W- treatments compared to optimal input supply (N + W+) in root and shoot tissues of IR64 and N22. (figshare.com)
  • I am interested in methods and applications of single cell gene expression assays. (mathalliance.org)
  • In Paper II , we present an assay for spatial RNA quality evaluation, used to estimate the success for tissue specimens before proceeding with more expensive spatial sequencing methods. (kth.se)
  • Thus, we bridge a gap between traditional tissue preservation methods and novel gene technologytools. (kth.se)
  • Here, we employed single-cell gene expression profiling to describe the dynamic transition between cell proliferative states in three different cell lines using a panel consisting of 93 marker genes. (frontiersin.org)
  • Assessing drug-induced changes in the expression of CYP genes provides a reliable predictive indicator of altered (either heightened or inhibited) metabolic activities leading to drug-drug interactions in vivo. (indigobiosciences.com)
  • To identify the most predictive genes we used a variable selection strategy. (frontiersin.org)
  • Detailed analysis of cell cycle predictive genes allowed us to define subpopulations with distinct gene expression profiles and to calculate a cell cycle index that illustrates the transition of cells between cell cycle phases. (frontiersin.org)
  • In conclusion, we provide useful experimental approaches and bioinformatics to identify informative and predictive genes at the single-cell level, which opens up new means to describe and understand cell proliferation and subpopulation dynamics. (frontiersin.org)
  • We also demonstrated that the −657 to +411 DCT promoter fragment efficiently directs RB cell-specific transcription of the luciferase reporter gene in cell lines. (molvis.org)
  • ChIP profiling and transcriptional reporter assays revealed that cholesterol increases PD-1 expression in part by increasing XBP1 occupancy at the Pdcd1 promoter. (aacrjournals.org)
  • Reporter gene assay of pck2 400 bp fragment containing GCGGAGGC motif (A) and myogenin fragment containing CGACCCGT motif (B). There is a change in the reporter activity upon mutagenesis in pck2 constuct and there is no change in myogenin construct. (biomedcentral.com)
  • Supplementary Table 3: Comparison of the expression of known transcription factors (TFs) under various stress treatments in IR64 and N22. (figshare.com)
  • Assaying the products of GENETIC TRANSCRIPTION in individual cells. (bvsalud.org)
  • As a transcription factor whose expression is increased by DNA damage, p53 blocks cell division at the G1 phase of the cell cycle to allow DNA repair. (medscape.com)
  • Diet-induced obesity led to altered acetylation of lysine 27 on histone H3 (H3K27ac) and to altered monomethylation of lysine 4 on histone H3 (H3K4me1) at numerous loci, with concomitant changes in the expression of genes that were functionally relevant to intestinal cancers. (biomedcentral.com)
  • Supplementary Table 7: Details of primers designed for validation of the DEGs identified from transcriptome analysis by qPCR assay. (figshare.com)
  • In a normal lymph node that displays both distinct gene expression patterns and histological landmarks, we observed a neutral iCNV profile. (kth.se)
  • CAMTAs participate in gene expression regulation by binding to the cis -elements in the promoter regions of numerous target genes. (frontiersin.org)
  • Expression-gene mapping, network analyses and chromatin looping nominated candidate disease-relevant target genes modulated by these daSNVs. (stanford.edu)
  • The results showed that nine ZmCAMTA genes showed highly diversified gene structures and tissue-specific expression patterns. (frontiersin.org)
  • Given the uncertainty about the relationship between these different forms, we compared their patterns of gene expression. (biomedcentral.com)
  • Because the regulation of gene expression has been found to be a common mechanism underlying ASD, the team will analyze gene expression patterns in the brains of mutants. (sfari.org)
  • What patterns of genes are expressed? (kth.se)
  • This technique has been well-optimized over the past decades and offers a robust option for miRNA profiling. (bitesizebio.com)
  • The classification, he explained, would need to have a "unique biology" and be reliably defined, using a "robust assay. (medscape.com)
  • The limitations and possibilities associated with expression profiling in intracellular amastigotes and promastigotes were assessed through a series of comparative experiments in which technical and biological parameters were scrutinized. (cambridge.org)
  • On the biological level, we demonstrate that gene expression levels fluctuate during in vitro development of both intracellular amastigotes and promastigotes. (cambridge.org)
  • We chose to use expression-curves rather than single, specific, time-point measurements to capture this biological variation. (cambridge.org)
  • This is why miRNA profiling is currently of immense biological interest and a rapidly growing field of study. (bitesizebio.com)
  • But, before you can begin to decipher how your miRNA-of-interest regulates a biological response, you will need a sensitive, reproducible and widely-accepted miRNA profiling platform to measure expression. (bitesizebio.com)
  • Find or custom design the right target-specific assays and panels to research your biological targets of interest. (qiagen.com)
  • Notably, a large number of stress-related elements present in the promoter regions of some ZmCAMTA genes, indicating a genetic basis of stress expression regulation of these genes. (frontiersin.org)
  • TP53 activates the expression of genes involved in apoptosis, cell cycle regulation (p21), and MDM2. (medscape.com)
  • Gene ID: 5925, OMIM 614041 ) gene, which encodes a tumor suppressor protein. (molvis.org)
  • This gene encodes a receptor that belongs to the protein tyrosine kinase Tie2 family. (thermofisher.com)
  • For example, the TP53 gene, located on chromosome 17, encodes a 53-kd nuclear protein that functions as a cell cycle checkpoint. (medscape.com)
  • We found that the total transcript level per cell and the expression of most individual genes correlated with progression through the cell cycle, but not with cell size. (frontiersin.org)
  • There is great hope, forgood reason, that measuring expression profiles in single cells will aid in immunological and cancer research,and provide new insight in cellular biology.However, the biochemical, computational and statisticalchallenges are sizable. (mathalliance.org)
  • Although several minerals have been shown to regulate gene expression and cellular activity, very little work has focused on understanding underlying molecular mechanisms. (sciencedaily.com)
  • In this assay, miRNAs in a heterogenous mixture of total cellular RNA are amplified using specific primers. (bitesizebio.com)
  • Each subtype has a characteristic expression pattern suggested to partly depend on cellular origin. (lu.se)
  • We examined the activities of cloned promoter DNA fragments with luciferase assay. (molvis.org)
  • For example, butyric acid has been found to up-regulate the expression of toll-like receptor 4 (TLR4) and the phosphorylation of MAPK and NK-κB in colon cancer cells in vitro ( 9 ). (iiarjournals.org)
  • 1997. In vitro genotoxic effects of hard metal particles assessed by alkaline single cell gel and elution assays. (cdc.gov)
  • In a single-center study reported in the Journal of Oncology Practice , Katya Losk, MPH , of Dana-Farber Cancer Institute, and colleagues found that an intervention including surgeon initiation of gene-expression profile testing with Oncotype DX significantly reduced the time to testing, receipt of testing results, and initiation of chemotherapy in patients with early-stage breast cancer. (ascopost.com)
  • 1. Losk K, Freedman RA, Lin NU, et al: Implementation of surgeon-initiated gene expression profile testing (Oncotype DX) among patients with early-stage breast cancer to reduce delays in chemotherapy initiation. (ascopost.com)
  • These results highlight several genes that may play an important role across the spectrum of serous ovarian tumorigenesis. (biomedcentral.com)
  • Innovative UMIs (unique molecular indices) eliminate PCR duplication and amplification bias to deliver the most accurate results (see figure Unbiased and accurate gene quantification (A) Different amounts (20 ng, 5 ng or 1.25 ng) of universal reference RNA were used to determine expression levels of three genes (BNIP1, CTNND2 and DAPK1) using targeted RNAseq. QIAseq digital RNA sequencing method (A; molecular barcode counts) showed accurate quantification of all three genes corresponding to different RNA input, whereas traditional targeted RNAseq (B; read counts) revealed PCR duplication limitation and yielded inaccurate quantification. "> Unbiased and accurate gene quantification ). (qiagen.com)
  • The unique combination of our proprietary primer design algorithm and rigorous testing of every primer assay guarantees high specificity and accurate results (see figure Proprietary primer design delivers gene-specific amplicons (>97% specificity) Sequencing libraries were prepared using 1.25, 5 or 20 ng universal reference RNA and QIAseq Targeted RNA Panels, ranging from 12-plex to 1000-plex. Sequencing was performed on the Illumina MiSeq, dedicating 1 million reads per sample. Specificity is calculated as percent of trimmed and mapped reads that map to intended targets. "> Proprietary primer design delivers gene-specific amplicons - 97% specificity ). (qiagen.com)
  • Targeted RNA sequencing using UMIs is optimized to deliver highly reliable quantification down to ~100 copies of an RNA target in 25 ng total RNA (see figure Positive results with as little as 0.2 copies of RNA per cell ERCC standards, at 86 to 705,500 copies, spiked into universal reference RNA sample and enriched using 384-plex QIASeq Targeted RNA Panel in three technical replicates. (A) Sensitivity measurement. Under standard conditions (20 ng RNA input, 0.5 million MiSeq reads), ≥~100 copies of ERCC transcripts were reliability detected, which is the equivalent of ~0.2 copies per cell. (B) Precision measurement. At >10 barcodes/gene, CV was less than 5% for all targets, indicating high technical reproducibility. This corresponds to ~100 copies target RNA in the sample. "> Positive results with as little as 0.2 copies of RNA per cell ). (qiagen.com)
  • Here we present ATAC with select antigen profiling by sequencing (ASAP-seq), a tool to simultaneously profile accessible chromatin and protein levels. (nature.com)
  • These include the T cell immunoglobulin and mucin-domain containing-3 (TIM-3), lymphocyte-activation gene-3 (LAG-3), TIGIT, and B-and T-lymphocyte-associated protein (BTLA) receptors associated with T cell exhaustion and V-domain immunoglobulin suppressor of T cell activation (VISTA), a receptor found on tumor-infiltrating myeloid cells. (biomedcentral.com)
  • The protein encoded by this gene is a member of the CMGC family of serine/threonine protein kinases. (cancerindex.org)
  • What does this gene/protein do? (cancerindex.org)
  • The new kit enables streamlined, multiomic characterization of cell populations so that researchers can expand their options at a larger scale and detect simultaneous gene and protein expression, 10x said. (genomeweb.com)
  • [ 1 ] His prediction was subsequently supported by the cloning of the retinoblastoma tumor suppressor gene ( RB1 ) and by functional studies of the retinoblastoma protein, Rb. (medscape.com)
  • Quantification of Total and Mutant Huntingtin Protein Levels in Biospecimens Using a Novel alphaLISA Assay. (lu.se)
  • Altered expression of this gene has been observed in multiple human cancers. (cancerindex.org)
  • Another important class of tumor suppressor genes involved in cell cycle control and in the generation of human cancers is the cyclin-dependent kinase (CDK) inhibitors. (medscape.com)
  • Many ZmCAMTA genes displayed high expression levels in the roots. (frontiersin.org)
  • Consequently, drugs that activate any of these xenobiotic sensing receptors can dramatically change the endogenous levels of CYP expression in the liver, potentially impacting the rate of their own metabolism, as well as the metabolism of all other co-administered drugs. (indigobiosciences.com)
  • Non-specific amplification can be used to increase the miRNA sample, but when measuring expression levels, this means you'll lose the direct correspondence between total input sample and the amounts of miRNAs measured, introducing non-linearity in the assay. (bitesizebio.com)
  • In Paper I , we describe a protocol for transcript profiling of single cells, capable of measuring the relative expression levels for genes of interest. (kth.se)
  • The distinction between 'less serious' effects and 'serious' effects is considered to be important because it helps the users of the profiles to identify levels of exposure at which major health effects start to appear. (cdc.gov)
  • A User's Guide has been provided at the end of this profile (see Appendix B). This guide should aid in the interpretation of the tables and figures for Levels of Significant Exposure and the MRLs. (cdc.gov)
  • Rather, current assays are designed to distinguish between disease with high levels and disease in which levels are not high. (medscape.com)
  • Regeneration is mediated by the molecular processes that regulate gene expression to control tissue renewal, restoration and growth. (sciencedaily.com)
  • The inhibition of cell proliferation was quantified using the WST-8 assay kit (Dojindo Molecular Technologies, Inc., Kumamoto, Japan) by spectrophotometrically measuring the absorbance (450 nm) of the water-soluble formazan formed via reduction by intracellular dehydrogenases. (iiarjournals.org)
  • Introduction: Five different molecular subtypes of breast cancer have been identified through gene expression profiling. (lu.se)
  • The eukaryotic epigenome is postulated to respond to environmental stimuli through alterations in chromatin features and, ultimately, gene expression. (biomedcentral.com)
  • Here, we profile the gut microbiome and chromatin characteristics in colon epithelium from mice fed either an obesogenic or control diet, followed by an analysis of the resultant changes in gene expression. (biomedcentral.com)
  • Kinetic and Thermodynamic Insights into Agonist Interactions with the Parathyroid Hormone Receptor-1 from a New NanoBRET Assay. (harvard.edu)
  • Alternative inhibitory receptors have been identified that may be targeted for anti-tumor immune therapy, such as lymphocyte-activation gene-3 (LAG-3), as have several potential target oncogenes for molecularly targeted therapy, such as tyrosine kinase inhibitors. (biomedcentral.com)
  • Supplementary Table 5: Comparison of plant hormone metabolism genes and their receptors under various stress treatments in IR64 and N22. (figshare.com)
  • BACKGROUND: Spinal and bulbar muscular atrophy (SBMA) is an X-linked recessive hereditary neuromuscular disorder caused by the expanded trinucleotide repeat in the androgen receptors gene. (bvsalud.org)
  • By finding the gene expression profiles for each of these features, her team is able to identify those profiles that "match" disease to biology to drug and more effectively treat patients. (cityofhope.org)
  • Our scientists pursue every aspect of cancer research-from exploring the biology of genes and cells, to developing immune-based treatments, uncovering the causes of metastasis, and more. (mskcc.org)
  • Profiling 2 to 4 single cells per patient and measuring gene-specific expression from targets previously associated with metastatic breast cancer supports the use of our protocol as a diagnostic tool. (kth.se)
  • Scholars@Duke publication: Nanostring-based multigene assay to predict recurrence for gastric cancer patients after surgery. (duke.edu)
  • We also identified an epigenetic signature common to all cases, with correlation to gene expression in 65% of these genes, suggesting that a core set of epigenetically deregulated genes is central to the initiation or maintenance of lymphoid transformation. (lu.se)
  • Gene expression profiling is increasingly used in the field of infectious diseases for characterization of host, pathogen and the nature of their interaction. (cambridge.org)
  • Gene expression analysis revealed that cholesterol exposure significantly increased the expression of ER stress-response genes, including XBP1. (aacrjournals.org)
  • Genetic analysis revealed an increased number (39 repeats) of tandem CAG repeats in the AR gene, which confirmed the diagnosis of SBMA. (bvsalud.org)
  • In this study, they analyzed whole transcriptomic sequencing (RNA-seq) data to evaluate the effect of nanosilicates and ionic dissolution products on the gene expression profiles of stem cells. (sciencedaily.com)
  • We then surveyed the distribution of stress-related cis -regulatory elements in the −1.5 kb promoter regions of ZmCAMTA genes. (frontiersin.org)
  • The regulatory elements required for this cell-specific gene expression are likely located within its proximal promoter. (molvis.org)