Bovine viral diarrhea virus (BVDV) is a single-stranded positive-sense RNA virus classified as a pestivirus belonging to the Flaviviridae family. (zoologix.com)
Detection2
Virus detection by PCR and RT-PCR revealed that CPV-2 was present in 48 (77.4%) samples, and no other agents were detected. (bvsalud.org)
On the other hand, molecular detection of the virus by PCR is a rapid, specific, sensitive and cost effective method for identifying viremic individuals (Weinstock, et al. (zoologix.com)
A reverse-transcription, loop-mediated isothermal amplification assay for detection of bovine ephemeral fever virus in the blood of infected cattle. (nih.gov)
Ultrasensitive detection of bovine respiratory syncytial virus by reverse transcription coupled real time PCR. (zoologix.com)
One-step multiplex real time RT-PCR for the detection of bovine respiratory syncytial virus, bovine herpesvirus 1 and bovine parainfluenza virus 3. (zoologix.com)
Its causative agent, bovine ephemeral fever virus (BEFV) is an enveloped, nonsegmented, single stranded negative sense RNA virus in the Ephemerovirus genus of the Rhabdoviridae family. (biomedcentral.com)
All classical endemic and emerging viruses, such as pestiviruses, bovine herpesvirus type 1, foot-and-mouth disease virus, bluetongue virus, epizootic hemorrhagic disease virus, Rift Valley fever virus, and bovine ephemeral fever virus, could be excluded as the causative agent. (cdc.gov)
BEFV1
A quantitative reverse-transcriptase real-time PCR assay, using TaqMan chemistry, for detecting bovine ephemeral virus (BEFV) is described. (nih.gov)
Occurrence2
Occurrence of bovine ephemeral fever in Okinawa Prefecture, Japan, in 2012 and development of a reverse-transcription polymerase chain reaction assay to detect bovine ephemeral fever virus gene. (nih.gov)
different populations of wild mice (mus musculus domesticus) in los angeles and ventura counties were observed over their lifespan in captivity for expression of infectious murine leukemia virus (mulv) and murine mammary tumor virus (mmtv) and for the occurrence of cancer and other diseases. (liverpool.ac.uk)
Livestock3
Rift Valley fever (RVF) is an arthropod-borne viral disease that affects people, livestock and wild animals. (cdc.gov)
We report here inter-epidemic period transmission of RVF in livestock population, evidenced by demonstration of RVFV antibodies in animals that were born after the 2006/07 RVF outbreak in Tanzania and demonstration of immunoglobulin M (IgM), a short lived class of antibodies, following infection by RVF virus in 9 samples. (cdc.gov)
and more -- see the avian & livestock test menu for a complete listing of avian and livestock assays. (zoologix.com)
Endemic1
These flaviviruses are endemic and epidemic to the northern and central areas of Australia, where surveillance involves year-round testing for seroconversions in sentinel chickens ( 1 ) and virus isolation from mosquito pools collected annually ( 2 , 3 ). (cdc.gov)
Cattle3
Bovine ephemeral fever (BEF), known as "three day sickness", is an arthropod-borne and acute viral infection of cattle and water buffalo. (biomedcentral.com)
In summer and autumn 2011, farmers and veterinarians in North Rhine-Westphalia, Germany, and in the Netherlands reported to the animal health services, local diagnostic laboratories, and national research institutes an unidentified disease in dairy cattle with a short period of clear clinical signs, including fever, decreased milk production, and diarrhea. (cdc.gov)
To identify the cause of the disease, we analyzed blood samples from affected cattle. (cdc.gov)
Viral3
Available G gene sequences of viral RNA were aligned, and primers and probes were designed to recognize the virus. (nih.gov)
Ten-fold dilutions of the run-off viral transcript were used as templates for the reaction in which they served as standards to quantitate unknown viral samples. (nih.gov)
Symptoms associated with the viral infection include high fever (104-108 F), depression, decreased feed intake, increased respiratory rate, cough, and nasal and lacrimal discharge. (zoologix.com)
Sequences3
To quantitate the viruses, cDNA containing the real-time amplicon was prepared with a forward primer carrying the T7 promoter sequences. (nih.gov)
In addition to the anticipated high number of host sequences, we detected in some samples a considerable portion of reads representing diverse bacterial species. (cdc.gov)
This inconsistency might have resulted from the lack of published Shamonda virus M and L segment sequences and does not necessarily indicate that the novel orthobunyavirus is a reassortant. (cdc.gov)
Isolation2
Virus isolation from all mosquito pools was performed as previously described ( 10 ). (cdc.gov)
BRSV is difficult to detect by culture, and the chances of isolation only improve when sampling animals that are in the incubation or acute phases of infection. (zoologix.com)
Infection3
Recently, it is the hotspot to study the relationship between miRNA regulation and virus infection. (biomedcentral.com)
We describe the detection and characterization of a novel flavivirus named Fitzroy River virus (FRV), isolated from mosquitoes collected in northern Western Australia, and seroepidemiologic evidence of human or animal infection. (cdc.gov)
Nine inhibition-ELISA positive samples were also positive for RVFV IgM antibodies indicating a recent infection. (cdc.gov)
Exposure3
Complications are usually related to the extent of virus exposure and patient health status, generally affecting children, pregnant women, and immunocompromised patients. (bvsalud.org)
The exposure status to RVF virus (RVFV) was determined using two commercial ELISA kits, detecting IgM and IgG antibodies in serum. (cdc.gov)
Initial exposure to the virus can result in severe respiratory disease, but subsequent exposures will only result in mild to subclinical disease due to developed immunity. (zoologix.com)
Evidence1
In recent years, evidence of Rift Valley fever (RVF) transmission during inter-epidemic periods in parts of Africa has increasingly been reported. (cdc.gov)
Host1
However, the reciprocal interaction between bovine ephemeral fever virus replication and host miRNAs still remain poorly understood. (biomedcentral.com)
Outbreak1
In this review, 50 questions regarding monkeypox virus (MPXV) and the current MPX outbreak were answered in order to provide the most updated scientific information and to explore the potential causes and consequences of this new health threat. (bvsalud.org)
Expression1
Additionally, the target association between miR-3470b and mitochondrial antiviral signaling protein (MAVS) was predicted by target gene prediction tools and further validated using a dual-luciferase reporter assay, and the expression of MAVS mRNA and protein levels was negatively associated with miR-3470b levels. (biomedcentral.com)
Australia1
Locations where Fitzroy River virus-positive mosquitoes were collected (black dots), Western Australia, Australia, 2011 and 2012. (cdc.gov)
Blood samples1
Figure 1 ), 3 blood samples obtained in October 2011 from dairy cows that had clinical signs at sampling ( Table , BH 80/11) were pooled and analyzed by using metagenomics. (cdc.gov)
Human1
Hepatitis B virus (HBV) is still a significant health problem in human. (bvsalud.org)
Cells1
This virus is named for its characteristic cytopathic effect on cell culture, the formation of syncytial cells. (zoologix.com)
Include1
Commonly isolated arboviruses include the flaviviruses MVEV (and subtype Alfuy virus), KUNV, Kokobera virus (KOKV), and Edge Hill virus (EHV) and the alphaviruses RRV, BFV, and Sindbis virus ( 4 , 5 ). (cdc.gov)
Family1
Bovine respiratory syncytial virus (BRSV) is an RNA virus belonging to the paramyxoviridiae family. (zoologix.com)