• In addition, flow cytometry and Transwell assays were used to evaluate the apoptosis and invasive ability of GC cells, respectively. (spandidos-publications.com)
  • Functional experiments demonstrated that knockdown of PTTG3P inhibited cell proliferation, migration and invasion, and promoted cell apoptosis, acting as an oncogene. (biomedcentral.com)
  • For example, circRNA_000166 can function as an oncogene by promoting the proliferation of CRC cells and by limiting apoptosis. (hindawi.com)
  • Growth inhibition was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and apoptosis was assayed by ELISA. (aacrjournals.org)
  • Glass -fiber-induced cell transformation and proto-oncogene activation in BALB/c-3T3 cells. (cdc.gov)
  • The possible mechanism of glass fiber induced transformation in relation to proto oncogene activation was also examined by cell transformation assay. (cdc.gov)
  • The projects feature state-of-the-art methods for the investigation of cell biology, such as flow cytometry and confocal microscopy, protein interaction analyses using the proximity-ligation assay and Biacore, and genetic manipulation employing the Cas9/CRISPR system. (lu.se)
  • The lack of reliable response assays reflective of in vivo tumor heterogeneity and associated resistance mechanisms hampers identification of reliable biomarkers. (nih.gov)
  • We used a series of multiplex assays to measure the concentrations of 55 serum analytes in specimens from patients from that outbreak to identify biomarkers specific to pediatric disease. (cdc.gov)
  • In this study, we used a series of multiplex assays to measure the concentrations of 55 serum analytes in specimens from patients from the Gulu outbreak to identify biomarkers that had age-specific associations with survival, hemorrhagic manifestations, or both. (cdc.gov)
  • The target interaction between miR‑195‑5p and circ‑PVT1 or ETS1 was predicted through bioinformatics analysis, and verified by dual‑luciferase reporter gene assay. (spandidos-publications.com)
  • Luciferase reporter assay and RNA pull-down assay were carried out to detect the interaction between miR-383 and LncRNA PTTG3P. (biomedcentral.com)
  • In addition, these alterations affect 3 principal categories of genes, as follows: proto-oncogenes, tumor suppressor genes, and DNA repair genes. (medscape.com)
  • This article briefly discusses tumor suppressor genes and then focuses on the role of proto-oncogenes in childhood cancer. (medscape.com)
  • ProductData": { "ID": "RTD001230", "ProductType": "Others", "BrandName": "VENTANA ® pan-TRK (EPR17341) Assay", "ProductNameAddition": "", "RegulatoryDisclaimer1": "For in vitro diagnostic use. (roche.com)
  • 2017). Modeling cancer driver events in vitro using barrier bypass-clonal expansion assays and massively parallel sequencing. (who.int)
  • The effects of PIP5K1α and its inhibitor on growth and invasion of BC were confirmed by using in vitro assays including. (lu.se)
  • We evaluated the reliability of a novel multiplex ligation-dependent probe amplification (MLPA) assay in detecting integration of human papillomavirus (HPV) based on the viral E2/E6 copy number ratio in formalin-fixed and paraffin-embedded cervical lesions. (dundee.ac.uk)
  • Transwell migration and invasion assays were used to examine cell migration and invasion abilities. (biomedcentral.com)
  • The fact that many Rho family effector proteins will specifically recognize the GTP bound form of the protein has been exploited experimentally to develop a powerful affinity purification assay that monitors RhoA protein activation. (cytoskeleton.com)
  • Language": "en", "Country": "XG", "Code": "Content" }, { "Name": "Intended Use", "Value": "VENTANA pan-TRK (EPR17341) Assay is intended for laboratory use in the qualitative immunohistochemical detection of the C-terminal region of the tropomyosin receptor kinase (TRK) proteins A, B and C by light microscopy in sections of formalin-fixed, paraffin-embedded tissue stained on a BenchMark IHC/ISH instrument. (roche.com)
  • Language": "en", "Country": "XG", "Code": "Intended Use" }, { "Name": "Background Information", "Value": "VENTANA pan-TRK (EPR17341) Assay is a rabbit monoclonal primary antibody directed against the C-terminal region of TRK A, B and C proteins, also known as pan-TRK. (roche.com)
  • It has been 20 years since investigators discovered the hyperlink between the Drosophila wingless (Wg) gene and the vertebrate oncogene int-1, thus establishing the family of signaling proteins known as Wnts. (ncbcs.org)
  • 4 ]. A relationship between activated or lymphocytes and enzyme-linked immuno- memory-effector lymphocyte populations sorbent assay (ELISA) for CD117. (who.int)
  • The Idylla EGFR prototype assay is a rapid and fully automated test which may easily be adopted by a wider number of pathological centres. (bmj.com)
  • This study assessed whether an Idylla EGFR prototype assay can be reliably applied to cytological lung cancer specimens. (bmj.com)
  • Methods The limit of detection (LOD) of the Idylla EGFR prototype assay was assessed by cell line dilution studies. (bmj.com)
  • A total of 10 ng was directly placed inside an Idylla EGFR prototype assay cartridge. (bmj.com)
  • Conclusions Idylla EGFR prototype assay is sensitive on extracted DNA and can reliably be applied on cytological samples, enabling implementation of EGFR testing even in less experienced diagnostic units. (bmj.com)
  • Western blot investigated the effect of EZH2 on levels of oncogenes such as HER-2, c-myc, PI3K, and Akt. (medscimonit.com)
  • Finally, innovative response assays, such as organoid cultures or patient-derived tumor xenografts, provide an extra dimension to correlate molecular profiles with drug efficacy and control cancer growth. (nih.gov)
  • As part of the deal, CRL will initially validate Nuclea's Oncogene Science 159 gene-based prostate cancer monitoring test. (genomeweb.com)
  • CRL will also validate Nuclea's Nuvera breast cancer assay and its US Food and Drug Administration-approved, serum HER-2/neu blood test. (genomeweb.com)
  • He carried out his postdoctoral training at the National Cancer Institute, NIH in the USA, where he established a role of p38MAPK in negative regulation of tumorigenesis and the key role of phosphatase Wip1 as a potent human oncogene - both directions are now widely pursued in the cancer field. (lifeboat.com)
  • Scientists have been working with very specific assays for very specific targets for years, explained Magnus Jansson, Ph.D., CSO of SymCel Sverige. (genengnews.com)
  • Since the viral and human genomes are distinct and HPV's oncogenes are well conserved in tumor tissue, they represent optimal targets for circulating tumor DNA assays," the authors wrote. (genomeweb.com)
  • B ) Immunofluorescence assay was used to detect EMT markers. (aging-us.com)
  • A standardized and reproducible protocol for serum-free monolayer culturing of primary paediatric brain tumours to be utilized for therapeutic assays. (lu.se)
  • In the present study, GC cell lines (HGC‑27 and AGS) were treated with various concentrations of propofol, and an MTT assay was performed to detect the cell viability. (spandidos-publications.com)
  • Cultures are subjected to a standard 2D viability assay to determine the health of the cells two days after treatment with the test compound. (genengnews.com)
  • The assay therefore provides a simple means of quantitating RhoA activation in cells. (cytoskeleton.com)
  • The RhoA activation assay was tested by loading the RhoA protein in cell lysates with either GTPγS or GDP. (cytoskeleton.com)
  • Results from BK036-S RhoA activation assay. (cytoskeleton.com)
  • These changes led to the constitutive activation of a prominent oncogene - receptor tyrosine kinase gene PDGFRA. (biospace.com)
  • Furthermore, the feasibility of detecting gain of the telomerase genes with the HPV MLPA assay was evaluated. (dundee.ac.uk)
  • The kit contains sufficient materials for 20 assays, depending on assay setup, and includes reagents for positive and negative controls. (cytoskeleton.com)
  • Language": "en", "Country": "XG", "Code": "Storage Conditions (Product)" }, { "Name": "Content", "Value": "VENTANA pan-TRK (EPR17341) Assay contains sufficient reagent for 50 tests. (roche.com)
  • Western blotting was performed to detect the protein levels of cyclin‑dependent kinase inhibitor P21 (P21), B‑cell lymphoma‑2 (Bcl‑2), matrix metalloproteinase 9 (MMP9) and E26 oncogene homolog 1 (ETS1). (spandidos-publications.com)
  • The assay sensitivity is what we do in the lab with limiting dilution of reagents, and we say, what's the least amount of molecules we can detect? (medscape.com)
  • We aim to validate a high performance functional assay for IRS prospectively. (biomedcentral.com)
  • 1 As a matter of the fact, the competence needed to validate molecular diagnostic assays is beyond most cytopathology laboratories and, even if there was expertise, a larger number of cases is required to run in-house testing cost-effectively. (bmj.com)
  • However, emerging evidence suggests that the lipid phosphatase SH2-domain-containing 5 inositol phosphatase (SHIP2), encoded by the INPPL1 gene, may act as an oncogene. (oncotarget.com)
  • It's below the assay detection limit, but they recur later. (medscape.com)
  • The advent of the assay for nucleic acid amplification and detection enables clinicians to initiate and monitor antiviral therapy whilst allowing basic scientists to carry out studies on HBV biology. (degruyter.com)
  • The MLPA results were compared with those of amplification of papillomavirus oncogene transcripts for RNA, detection of integrated papillomavirus sequences for DNA, and HPV fluorescence in situ hybridization (FISH). (dundee.ac.uk)
  • From 13 matching frozen samples, DNA and RNA were isolated for the detection of integrated papillomavirus sequences and/or the amplification of papillomavirus oncogene transcripts, respectively. (dundee.ac.uk)
  • In conclusion, the HPV MLPA assay can be performed on routinely processed cervical lesions for the detection of viral load and HPV integration. (dundee.ac.uk)
  • Especially in drug discovery, most such assays have been geared toward a particular reaction-whether it is recognition of a cell surface marker, triggering endocytosis, or exciting or inhibiting a phosphorylation cascade. (genengnews.com)
  • He said there was a huge interest in label-free assays among the participants of the recent SMi Conference on "Cell Based Assays" in London, where he discussed SymCel's pending entry into the field. (genengnews.com)
  • The company has developed a calorimetry-based assay system that can monitor the total metabolic activity by measuring the heat flow in and out of a cell culture well in real time, down to the microwatt level. (genengnews.com)
  • SymCel's calPlate assay is independent of cell type and media conditions. (genengnews.com)
  • SymCel calScreener sample vials in close up: calScreener offers calorimetry-based cell assays in a microtiter plate format. (genengnews.com)
  • and cell differentiation ( SOX2 and TGFB3 ) as well as immunohistochemical assay for VEGFA, TP53, Bcl2, TGFB1, and Ki67 protein expression have been performed in 85 FFPE RCC tumor specimens. (hindawi.com)
  • NOTE: Cell culture supernatant samples can be run without diluting in Assay Buffer if very low levels (less than 20 pg/mL) of cytokines are expected. (assaygenie.com)
  • The MLPA assay detected viral integration in 12 of these 13 cases, and episomal copies also were detected in 7 cases. (dundee.ac.uk)
  • Orthologous to human ABL2 (ABL proto-oncogene 2, non-receptor tyrosine kinase). (nih.gov)
  • Electrophoretic mobility shift assay was used to evaluate the DNA binding activity of NF-κB. (aacrjournals.org)
  • It's not necessary to know anything about your system with regard to the receptor, nor what kind of assay you're going to run to monitor that activity," he pointed out. (genengnews.com)
  • Say you have a G-protein coupled receptor (GPCR) of unknown function-with this assay you can determine whether it effects a stimulatory or inhibitory response, or induces necrosis under your experimental conditions, Dr. Jansson proposed as an example. (genengnews.com)
  • Because the assays require no labels they are noninvasive and nondestructive to the cells, inexpensive, simple, and quick to set up and use. (genengnews.com)
  • The effect on tumorigenesis potency of glioma stem cells was determined by mouse transplantation assay. (medscimonit.com)
  • And due to lack of gas exchange there is an upper limit on the length of an assay as well-at least in aerobic cultures. (genengnews.com)
  • Later that decade, similar patterns of biochemical and oncogene expression were observed. (medscape.com)
  • One 5 mL dispenser of VENTANA pan-TRK (EPR17341) Assay contains approximately 140 μg of a rabbit monoclonal antibody. (roche.com)
  • VENTANA pan-TRK (EPR17341) Assay is produced as a Protein A purified recombinant rabbit monoclonal antibody. (roche.com)
  • Idylla results were compared with fragment length (exon 19 del) and Taqman assays. (bmj.com)
  • The rise in geriatric population will further compound the growing demand for malignancy assays and the rapid market expansion. (marketresearch.com)