Assay hla c antigens. Medical search. Frequent questions

In this study, we comprehensively screened HLA-A2 restricted MAGE-A3 tumor epitopes and characterized the corresponding TCRs using in vitro artificial antigen presentation cells (APC) system, single-cell transcriptome and TCR V(D)J sequencing, and machine-learning. (thno.org)
We identified the HLA-A2 restricted T cell epitopes from MAGE-A3 that could effectively induce the activation and cytotoxicity of CD8+ T cells using artificial APC in vitro . (thno.org)
A cohort of HLA-A2+ NSCLC donors demonstrated that the number of epitope specific CD8+ T cells increased in NSCLC than healthy controls when measured with tetramer derived from the candidate MAGE-A3 epitopes, especially epitope Mp4 (MAGE-A3: 160-169, LVFGIELMEV). (thno.org)
Epitopes are a component of the antigen that are recognized by the immune system and determine whether the cellular or the humoral arm of the immune system shall be activated against that particular antigen. (medscape.com)
Immunoglobulins (Igs), the term is sometimes used interchangeably with "antibodies," are glycoprotein molecules produced by B lymphocytes and plasma cells in response to an immunogen or after recognition of specific epitopes on the antigen. (medscape.com)
AIM: To identify CD4(+) T-cell epitopes within Der p 2 recognized by commonly expressed human leucocyte antigen (HLA) alleles. (ox.ac.uk)
METHODS: HLA-blocking antibodies, peptide pools and truncations were used in ELISpot assays to establish restricted T-cell epitopes. (ox.ac.uk)
MAPPS) assays use purified HLA-II peptides of the protein of interest to complement in silico identification and evaluation of the binding affinity of HLA-II peptide epitopes. (ddw-online.com)
With current knowledge of epitopes, it is more accurate to describe that antibodies bind to their target epitopes on the surface of HLA molecular chains. (mundial-brasil2014.net)
The phenomenon of cross-reactivity in HLA testing, often explained as cross-reactive groups (CREGs) of antigens with antibody, can be clearly explained now by public epitopes. (mundial-brasil2014.net)
Since 2006, we defined and reported 194 HLA class I unique epitopes, including 56 cryptic epitopes on dissociated HLA class I heavy chains, 83 HLA class II epitopes, 60 epitopes on HLA-DRB1, 15 epitopes on HLA-DQB1, 3 epitopes on HLA-DQA1, 5 epitopes on HLA-DPB1, and 7 MICA epitopes. (mundial-brasil2014.net)
however, it is more accurate to describe the reactivity of the antibody as binding to specific epitopes on the surface of HLA antigensepitopes are conformational amino acid arrangements and are the targets of antibodies. (mundial-brasil2014.net)
others are public epitopes shared by two or more antigens. (mundial-brasil2014.net)
Although a limited number of immunodominant peptide epitopes are consistently observed in diseases such as HIV-1 infection, the relationship between immunodominance and antigen processing in humans is largely unknown. (jci.org)
We used a novel in vitro degradation assay involving cytosolic extracts as well as endogenous intracellular processing assays to examine 2 well-characterized HIV-1 Gag overlapping epitopes presented by the same HLA class I allele, one of which is consistently immunodominant and the other subdominant in infected persons. (jci.org)
Studies of these responses have largely relied on the analysis of a small number of previously described or predicted HCV epitopes, mostly restricted by HLA A2. (ox.ac.uk)
Only four subjects had detectable HLA A2-restricted responses in PBMC, and only 3 of 19 predicted A2 epitopes were targeted, all of which were confirmed by tetramer analysis. (ox.ac.uk)
Since responses to epitopes restricted by single HLA alleles such as HLA A2 do not predict the overall response in an individual, more comprehensive approaches, as shown here, should facilitate definition of the role of the CD8(+)-T-cell response in HCV infection. (ox.ac.uk)
These peptides (HLA-II T-cell epitopes) are increasingly important as research targets for the development of vaccines and immunotherapies. (biomedcentral.com)

Disease associated human leukocyte antigen (HLA) genes have been identified in humans where they are assumed to promote the susceptibility and/or progression of rheumatoid arthritis. (hu-berlin.de)
Initiation and regulation of immune responses in humans involves recognition of peptides presented by human leukocyte antigen class II (HLA-II) molecules. (biomedcentral.com)
These therapeutic methods, including but not limited to oncolytic virus therapies, T-cell therapies and cancer vaccines, are based on the body's ability to recognize mutated antigen peptides presented on the cell surface by MCH-receptors (also known as HLA-receptors in humans) and the disposal of the malignant cells by cytotoxic T-cells. (helsinki.fi)

For intracellular pathogens, the antibody titers and neutralization assays represent routine immunologic tests that provide results after several weeks of infection. (cdc.gov)
The new, enhanced LSA Assays deliver increased coverage, optimized performance, and improve the overall workflow of the LIFECODES® HLA Antibody Analysis portfolio. (immucor.com)
LIFECODES offers the complete antibody product portfolio for the transplant laboratory, including kits designed to detect and identify non-HLA antibodies in serum. (immucor.com)
Antibody identification and panel-reactive antibody (PRA) information are derived from native antigens. (immucor.com)
The G46-6 monoclonal antibody specifically binds to HLA-DR, a major histocompatibility complex (MHC) class II antigen. (bdbiosciences.com)
Blood group typing market is further segmented by test type: human leukocyte antigen (HLA) typing, antibody screening, ABO blood tests, cross-matching series, antigen typing. (gen-cons.com)
Variable region constitutes the antibody binding region of the molecule to the different antigens as it consists of about 110 amino acids that vary widely among the different antibody molecules. (medscape.com)
The L243 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. (biolegend.com)
internal QC demonstrates detection of Hla protein using IBT's mouse anti-Alpha Toxin (6C12) monoclonal antibody (cat# 0210-005) in Western blotting. (gentaur-italy.com)
Advances in testing assays and amino acid sequencing of HLA along with computer software contributed further to the understanding of antibody-antigen reactivity. (mundial-brasil2014.net)
Determining specificity of the anti-HLA antibody has advanced in recent years using recombinant CHMFL-ABL-039 HLA single antigens (SA) coated on color-coded Luminex beads [3]. (mundial-brasil2014.net)
Amino acid sequences of the HLA molecules which greatly contributed to our understanding of antibody and antigen reactivity has been introduced since 1963 [4C12]. (mundial-brasil2014.net)
The aims of this study were to investigate to which extent HLA-DSA detected by SAFB represent a risk for antibody-mediated rejection (AMR) and diminished allograft survival, and to define HLA-DSA characteristics predictive for AMR. (unibas.ch)
This antibody reacts with native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. (genetex.com)
The antibody MEM-G/9 is standard reagent thoroughly validated during 3rd International Conference on HLA-G (Paris, 2003). (genetex.com)
FACS analysis of HLA-G1 transfectants (LCL-HLA-G1) using GTX78335 HLA-G antibody [MEM-G/9] (PE). (genetex.com)
There are currently no reviews for HLA-G antibody [MEM-G/9] (PE) (GTX78335) . (genetex.com)

Among them the differential expression of HLA-DRB molecules in different types of antigen-presenting cells, which was investigated here in detail. (hu-berlin.de)
Antigens are the molecules that are recognized and stimulate the cells of immune system. (medscape.com)
In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4 + T cells. (biolegend.com)
HLA-DR molecules were affinity purified and bound peptides acid eluted. (jci.org)
Peptide and peptide mimetic inhibitors of antigen presentation by HLA-DR class II MHC molecules. (rcsb.org)
Molecular features of ligand binding to MHC class II HLA-DR molecules have been elucidated through a combination of peptide structure-activity studies and structure-based drug design, resulting in analogues with nanomolar affinity in binding assays. (rcsb.org)
These studies illustrate the complementary roles played by phage display library methods, peptide analogue SAR, peptide mimetics substitutions, and structure-based drug design in the discovery of inhibitors of antigen presentation by MHC class II HLA-DR molecules. (rcsb.org)
Antigen variation in pathogens and tumor antigens, and extensive polymorphism of HLA molecules increase the number of targets for screening studies. (biomedcentral.com)
Experimental screening methods are expensive and time consuming and reagents are not readily available for many of the HLA class II molecules. (biomedcentral.com)
Full overlapping studies of four antigens identified binding affinity of 103 peptides to seven common HLA-DR molecules (DRB1*0101, 0301, 0401, 0701, 1101, 1301, and 1501). (biomedcentral.com)
Because not all servers have predictors for all tested HLA-II molecules, we assessed a total of 113 predictors. (biomedcentral.com)
CTLs recognize peptide antigens presented by major histocompatibility complex class I (MHC-I) molecules on infected cells or cancer cells and kill them. (biomedcentral.com)
MHC-II molecules present antigenic peptides internalized by professional antigen presenting cells, such as macrophages, dendritic cells, or T lymphocytes. (biomedcentral.com)

BACKGROUND: Defining the clinical relevance of donor-specific HLA-antibodies detected by single-antigen flow-beads (SAFB) is important because these assays are increasingly used for pretransplant risk assessment and organ allocation. (unibas.ch)

For the first time, to our knowledge, we have identified HLA-bound peptides presented in vivo during an inflammatory condition. (jci.org)

This technique requires that whole proteins or selected peptide antigens are added to blood cells, allowing the simultaneous analysis of both major histocompatibility complex class I and II restricted T-cell responses ( 7 ). (cdc.gov)
Background Post-translational modification of proteins has the potential to alter the ability of T cells to recognize major histocompatibility complex (MHC) class -I and class-II restricted antigens, thereby resulting in altered immune responses. (bmj.com)

With few exceptions, mothers of subjects with persistent maternal microchimerism were HLA incompatible with subjects for class I and class II alleles. (jci.org)
Identification of an immunodominant region of the major house dust mite allergen Der p 2 presented by common human leucocyte antigen alleles. (ox.ac.uk)
Within one pool of Der p 2 peptides, the 20mer D11 was found to induce multiple responses restricted through several alleles, including HLA-DPB1*0401 and HLA-DRB1*01. (ox.ac.uk)
CONCLUSIONS: We have identified an immunogenic region of Der p 2 presented by common HLA class II alleles, including the most commonly expressed HLA allele DPB1*0401. (ox.ac.uk)
Of course, the vast diversity of HLA alleles makes it difficult to perfectly predict the immunogenicity of a therapeutic with respect to the entire human population. (ddw-online.com)
Hence, most in vitro risk prediction should of necessity involve a population of donors with diverse HLA alleles. (ddw-online.com)
In order to determine the actual breadth and magnitude of CD8(+)-T-cell responses in the context of diverse HLA class I alleles, we performed a comprehensive analysis of responses to all expressed HCV proteins. (ox.ac.uk)

However, systematic analysis of T cell immune responses to MAGE-A3 antigen and corresponding antigen-specific TCR is still lacking. (thno.org)

ELISA is used to confirm the presence of HLA-I receptors in the ligandome affinity purification eluates and to estimate the efficacy of purification. (helsinki.fi)
Bound proteins were purified by DNA affinity precipitation assays (DAPA) and eluted proteins analyzed by mass spectrometry (MS), demonstrating that rs14798008 bound H1 histones. (cdc.gov)

RESULTS: In 50/59 CD subjects, irrespective of homo- or heterozygosity for HLA-DQ2, IFN-gamma ELISPOT responses for an optimal concentration of A-gliadin 57-73 Q-E65 were between 10 and 1500 per million PBMC, equivalent to a median 51% of the response for a "near optimal" concentration of deamidated gliadin. (ox.ac.uk)
4. Immunological assays (ELISA, ELISPOT). (helsinki.fi)
2. Interferon γ cytokine secretion was determined by ELISPOT to assess PBMC response against known antigens in a preliminary experiment to approximate usable range for the following antigen specific PBMC assays. (helsinki.fi)

Use of microimmobilization and microagglutination assays for attempted detection of HLA antigens and beta2 microglobulin on human sperm. (ox.ac.uk)
These assays are proved to be useful in the study of anti-sperm antibodies in the sera of vaseectomized men and the serum of a rhesus monkey immunized with human sperm. (ox.ac.uk)
HLA-DQ9 (DQ9) is a human leukocyte antigen serotype within the HLA-DQ (DQ) serotype group. (wikipedia.org)
We developed sensitive human leukocyte antigen-specific (HLA-specific) PCR assays and targeted nonshared maternal HLA genes to test for persistent maternal microchimerism in subjects with scleroderma and in healthy normal subjects. (jci.org)
FCXM assays are more widely applied across human leukocyte antigen (HLA) labs. (curiox.com)
dashed line histogram) or with Purified Mouse Anti-Human HLA-DR (Cat. (bdbiosciences.com)
The only-antigen bead assay (SABA) demonstrates excessive sensitivity and specificity for detecting anti-human leukocyte antigen (HLA) antibodies. (microsensbp.com)
This partnership validates our approach of using synthesized human T cell repertoires to generate safe and effective libraries to discover antigen specific TCRs at scale," said Elizabeth Wood, Ph.D., Founder & CEO, JURA Bio. (businesswire.com)
Overall primary concerns are: extrapolating human clinical studies, seeding durable effective T cell resident memory (Trm), population human leucocyte antigen (HLA) coverage, and the potential for T cell-mediated immune escape. (mdpi.com)
Usually, human primary immune cells are employed for in vitro biological assays for immunogenicity prediction, and though they are generally obtained from healthy donors, samples from specific subpopulations of patients can be of interest in the study of disease-specific immunogenic reactions. (ddw-online.com)
Here we report, using the pathogenic model of antiretroviral therapy-treated, SIV-infected rhesus macaques that sequential interleukin-21 and interferon alpha therapy generate terminally differentiated blood natural killer cells (NKG2a/c low CD16 + ) with potent human leukocyte antigen-E-restricted activity in response to SIV envelope peptides. (nature.com)
Methods Homocitrullinated peptides were identified and assessed in vitro for HLA-A2 binding and in vivo in human leukocyte antigen (HLA) transgenic mouse models for immunogenicity. (bmj.com)
Recombinant human HLA-G refolded with beta2-microglobulin and peptide. (genetex.com)
It has been suggested that cytokine acc leukoreduction prevents transmission of cyc cumulation in plasma of platelet concenc tomegalovirus and decreases alloimmunizac trates (PC) during storage may contribute tion to human leukocyte antigen (HLA), it to development of nonchaemolytic febrile does not prevent allergic reactions. (who.int)
Predictive value for human leukocyte antigen-typing. (medscape.com)

Baines M, Ebringer A, Avakian H et al (1990) The use of enzyme immunoassay (EIA) and radiobinding assay to investigate the cross-reactivity of Klebsiella antigens and HLA-B27 in ankylosing spondylitis patients and healthy controls. (springer.com)
The reactivity of anti-HLA antibodies with HLA antigens and the phenomenon of cross-reactivity has been the subject of investigation for decades. (mundial-brasil2014.net)
Reactivity with HLA-G3 was also reported. (genetex.com)

Clone L243 binds a conformational epitope on HLA-DRa which depends on the correct folding of the aß heterodimer. (biolegend.com)
Figure G. Epitope 5037 shared by the HLA C-locus antigens Cw4, Cw6, Cw17, Cw18 and defined by 73A+77N. (mundial-brasil2014.net)
Figure H HLA class II DQB epitope 2007 shared by DQ4,5,6 antigens and define by 52P+55R on the beta chain of the DQ antigens. (mundial-brasil2014.net)
Figure J. Epitope 2018 shared by the alpha chains of the DQ4, 5, 6 antigens and defined by Glutamine (Q) at position 53. (mundial-brasil2014.net)
Figure K. Epitope 2002 shared exclusively by the beta chains of the DQ4 antigen and defined by Leucine (L) in position 56. (mundial-brasil2014.net)
Figure L. Epitope 2010 shared by the beta chains of DQ antigens DQ4, 5, 6, 8, 9 and defined by 45G+46V. (mundial-brasil2014.net)
Figure M. Epitope 2022 exclusive to DQB1?05:01 chain on the DQ5 antigen and defined by 125S+126Q. (mundial-brasil2014.net)
Figure N. Epitope 2006 shared by DQB1?03:01 (DQ7), DQB1?03:02 (DQ8), DQB1?03:03 (DQ9) and defined by Proline (P) at position 55 on the beta chains of the DQ antigens. (mundial-brasil2014.net)
AIMS: To determine the contribution of a putative dominant T cell epitope to the overall gliadin T cell response in HLA-DQ2 CD in vivo. (ox.ac.uk)
Whole deamidated gliadin and gliadin epitope specific T cells induced in peripheral blood expressed an intestinal homing integrin (alpha4beta7) and were HLA-DQ2 restricted. (ox.ac.uk)
Currently available HLA-II prediction servers offer only a limited prediction accuracy and the development of improved predictors is needed for large-scale studies, such as proteome-wide epitope mapping. (biomedcentral.com)
In silico methods were successfully used to characterize previously identified HLA-restricted peptides and one previously identified immunogenic T-cell epitope. (helsinki.fi)

Detect C3d-binding donor specific anti-HLA antibodies (DSAs) for better risk stratification of patients. (immucor.com)
Sensitization is almost ubiquitous as anti-HLA antibodies are found in almost all sera of transplant recipients. (mundial-brasil2014.net)
Sensitization is almost ubiquitous as it is evident in the detection of anti-HLA antibodies in the sera of recipientsin one study, almost all patients waiting for regraft of a kidney transplant have anti-HLA antibodies [2]. (mundial-brasil2014.net)

PBMC, DC:T) assay makes use of APC surface expression of specific antigens, eg. (ddw-online.com)
The antigen priming of CD4+ T cells by mature MoDCs leads to the proliferation of CD4+ T cells in in-vitro PBMC assays. (ddw-online.com)
By using a panel of 301 overlapping peptides, we analyzed peripheral blood mononuclear cells (PBMC) from a cohort of 14 anti-HCV-positive, HLA A2-positive individuals in an enzyme-linked immunospot assay. (ox.ac.uk)
Preliminary MTS assay is used to determine linear range and optimal time frame for the PBMC/cancer cell co-culture killing assay. (helsinki.fi)

This work identified candidate TCRs potentially suitable for TCR-T design targeting HLA-A2 restricted MAGE-A3 tumor antigen. (thno.org)
In this study, we found that cross-presentation of tumor antigen by tumor endothelial cells(tECs) promotes infiltration of tumor specific CTLs in tumors. (nii.ac.jp)
We developed in vivo method to evaluate the infiltration of tumor specific CTLs in tumors by recognition of tumor antigen on tECs. (nii.ac.jp)

Identification of HLA-DP polymorphism with DP alpha and DP beta probes and monoclonal antibodies: correlation with primed lymphocyte typing. (ox.ac.uk)

Expand your knowledge of HLA antibodies and molecular typing. (thermofisher.com)
Report high-quality Next Generation Sequencing (NGS) HLA Typing results, with an optimized workflow, in less time than ever before using the MIA FORA NGS MFlex HLA Typing Kit. (immucor.com)
Perform HLA SSO typing with a ready-to-use kit and rapid protocol. (immucor.com)
The global market for blood group typing can be segmented by technology: PCR-based and microarray technique, massively parallel sequencing, assay-based techniques, others. (gen-cons.com)
Thirty-four lymphoblastoid cell lines that had been previously typed for HLA-DP antigens by primed lymphocyte typing (PLT) were tested by Southern blotting and by ELISA. (ox.ac.uk)

Hla), also known as alpha toxin (α-toxin), is expressed in E. coli as a recombinant protein without TAG. (gentaur-italy.com)

Detect donor-specific HLA antibodies against Class I and/or Class II antigens. (immucor.com)

ELISA: Assay-dependent dilution. (gentaur-italy.com)

Flow cytometric analysis of T-cell responses to smallpox antigens after recent smallpox vaccination and in long-term vaccinated or not vaccinated persons. (cdc.gov)
CCK-8 assay, flow cytometric analysis, western blotting, orthotopic GBM model as well as transwell assay were performed to investigate the biological role of CASP5. (cancerindex.org)
Flow cytometric analysis of HLA-DR on Rhesus macaque (Macaca mulatta) lysed whole blood. (bdbiosciences.com)
Flow cytometric analysis provided essential information of the cell line HLA-1 expression. (helsinki.fi)

However, using various antisera against beta2 microglobulin and HLA region antigens, including Ia antigens, in these assays, very little, if any, activity was found against sperm. (ox.ac.uk)
Although no HLA region antigens could be detected in the seminal plasma by inhibition tests, a large amount of beta2 microglobulin was found. (ox.ac.uk)
The maximum amounts of HLA region antigens and beta2 microglobulin in the seminal plasma are estimated. (ox.ac.uk)

A local outbreak of orthopoxvirus infection would require rapid and sensitive diagnostics, including novel assays based on host responses. (cdc.gov)
Moreover, antigen-specific T-cell responses could be detected in exposed, but uninfected persons, as shown in those with HIV infection ( 3 ). (cdc.gov)
We identified some features typical of elite controllers, including high magnitude HIV-specific responses and beneficial HLA. (biomedcentral.com)
Modified peptides showed enhanced binding to HLA-A2 compared with the native sequences and immunization of HLA-A2 transgenic mice generated high avidity modification specific CD8 responses that killed peptide expressing target cells. (bmj.com)

The goal of vaccination is to induce immunity against pathogens and cancer cells by stimulating antigen-specific cytotoxic T lymphocytes (CTLs) or B cells. (biomedcentral.com)

METHODS: In this retrospective study of 334 patients with negative complement-dependent cytotoxicity crossmatches, day-of-transplant sera were analyzed by SAFB, HLA-DSA determined by virtual crossmatching, and the results correlated with the occurrence of AMR and allograft survival. (unibas.ch)

The protein can be also used in serology assay as coating antigen to detect antibodies against Hla. (gentaur-italy.com)

Biologic drugs as therapy have improved the treatment of many diseases, but their success is often tempered by antidrug antibodies (ADAs), which specifically recognise the therapeutic molecule as a foreign antigen. (ddw-online.com)

Braun J, Bollow M, Remlinger G et al (1998) Prevalence of spondylarthropathies in HLA B27-positive and negative blood donors. (springer.com)

Watch this on-demand webinar to see Dr. Jakob Nilsson discuss a new NGS-based assay used to detect donor-derived cell-free DNA from grafts when monitoring patients after a kidney transplantation. (thermofisher.com)
1. Introduction Sensitization to HLA antigens in organ transplant patients causes graft rejection, according to the humoral theory of transplantation [1]. (mundial-brasil2014.net)
We have previously shown that Scandinavian sarcoidosis patients expressing the HLA-DR allele DRB1*0301 are characterized by large accumulations in the lungs of CD4+ T cells expressing the TCR AV2S3 gene segment. (jci.org)
To identify candidates for the postulated sarcoidosis-specific antigen, lung cells from 16 HLA-DRB1*0301pos patients were obtained by bronchoalveolar lavage. (jci.org)
peptides bound to HLA-DR17 from bronchoalveolar lavage cells of sarcoidosis patients were analyzed in order to identify target antigens of the autoimmune response (see the related article beginning on page 3576). (jci.org)
PATIENTS: HLA-DQ2+ individuals with CD and healthy controls. (ox.ac.uk)
RESULTS: Sixty-seven of 334 patients (20%) had HLA-DSA. (unibas.ch)
Notably, 30/67 patients with HLA-DSA (45%) did not experience clinical/subclinical AMR. (unibas.ch)

The body's immune system can react adaptively against the antigen via 2 pathways: cellular or humoral. (medscape.com)
The cellular response is mainly a lymphocyte-mediated reaction, whereas the humoral response includes production of antibodies against the antigen by the plasma cells. (medscape.com)
Isolated cellular subsets can allow better control in terms of assay set-up, but care must be taken to ensure minimal cellular damage, maximum yield, and standardised processing. (ddw-online.com)

Both elite controllers expressed multiple HLA Class I and II haplotypes previously associated with slower disease progression (HLA-A*74:01, HLA-B*44:03, HLA-B*81:01, HLA-B*57:03, HLA-DRB1*13). (biomedcentral.com)
The number, class, and cumulative strength of HLA-DSA determined by SAFB, and prior sensitizing events were not predictive for the occurrence of AMR. (unibas.ch)
HLA-G belongs to the HLA class I heavy chain paralogues. (genetex.com)
The initial NIOSH R01 grant award (2006-2012) identified candidate variants (snps) linked to Th2 cytokines, antioxidant enzymes, and HLA class I and II antigens. (cdc.gov)

To determine the impact of the promoter elements on the transcriptional activities measured by luciferase assay the surface plasmon resonance technology was employed. (hu-berlin.de)
A luciferase reporter assay, in A549 cells, identified allele-dependent mRNA transcription. (cdc.gov)

CONCLUSIONS: These results support the utility of SAFB for pretransplant risk assessment and organ allocation, and suggest that improvement of the positive predictive value of HLA-DSA defined by SAFB will require an enhanced definition of pathogenic factors of HLA-DSA. (unibas.ch)

Several mechanisms have been described how these HLA haplotypes impact on the disease. (hu-berlin.de)
The promoters of the disease associated HLA-DR4 to the neutral DR7 and DR9 haplotypes were analyzed for sequence polymorphisms resulting in functional differences. (hu-berlin.de)

Adoptive cell therapy using engineered T-cell receptors (TCRs) targeting cancer-testis antigens, such as Melanoma-associated antigen 3 (MAGE-A3), is a potential approach for the treatment of NSCLC. (thno.org)

Cytotoxicity assay: The protein is active in functional lysis assays with rabbit red blood cells. (gentaur-italy.com)

Fitness assays revealed that both women were infected with replication competent viruses, and both expressed higher mRNA levels of p21 , a host restriction factor associated with viral control. (biomedcentral.com)
HLA-II peptide binding studies involve multiple overlapping peptides spanning individual antigens, as well as complete viral proteomes. (biomedcentral.com)

This approach can be extended to characterize HLA-bound peptides in various autoimmune settings. (jci.org)

Using a rapid flow cytometric test, we previously showed that monitoring interferon (IFN)-γ production by antigen-pulsed T cells provides a quantitative and functional assessment of HIV- or cytomegalovirus (CMV)-specific CD8(+) and CD4(+) T cells ( 4 - 6 ). (cdc.gov)
HLA-specific PCR also frequently revealed persistent maternal microchimerism in healthy control subjects. (jci.org)
This association afforded us a unique opportunity to identify a sarcoidosis-specific antigen recognized by AV2S3+ T cells. (jci.org)

Good performance predictors included HLA-DRB1*0101 (seven), 1101 (six), 0401 (three), and 0701 (one). (biomedcentral.com)

These cells allow different types of assays to be performed, covering the T cell-mediated immune response from antigen uptake to T cell proliferation. (ddw-online.com)

Fluorescent histograms showing expression of HLA-DR (or Ig isotype staining) were derived from gated events based on forward and side light scattering characteristics for intact lymphocytes. (bdbiosciences.com)

Absorption and inhibition tests also could not show a significant amount of these antigens on sperm. (ox.ac.uk)

Electrophoretic mobility shift assays (EMSA) identified oligonucleotide-protein binding for risk and non-risk SNPs to nuclear extracts of A549, BEAS 2B, and IMR-90 lung cell lines. (cdc.gov)

These results clearly indicate that HLA-disparate maternal cells can persist in immunocompetent offspring well into adult life. (jci.org)
Autologous mature dendritic cells derived from peripheral blood monocytes were pulsed with the HLA-A2-binding MUC1 peptides (M1.1 and M1.2). (aacrjournals.org)
Proinflammatory islet antigen reactive CD4 T cells are linked with response to alefacept in type 1 diabetes. (immunetolerance.org)
They can also stimulate the destruction of antigen by cells like macrophages, neutrophils, and eosinophils by covering the surface of the antigen by the Fc portion of the antibodies in a process known as "opsonization. (medscape.com)
This master thesis is a part of a project aiming to set up immunoaffinity-purification/MS based method in order to analyse the ligandome and determine T-cell recognized cancer associated antigens from tumor cells. (helsinki.fi)
HLA-G is expressed on fetal derived placental cells. (genetex.com)
26 (3):106089 CAR-T cells targeting HLA-G as potent therapeutic strategy for EGFR-mutated and overexpressed oral cancer. (genetex.com)

Besides, results showed cardiac arrest (uncontrolled vs. controlled, HR 2.49, P = 0.049), HLA mismatch (4-6 loci vs. 0-3 loci, HR 3.61, P = 0.039), and AR occurrence (HR 2.91, P = 0.006) were demonstrated to be independent risk factors for graft loss. (biomedcentral.com)

Anatomy13

Organisms5

Chemicals and Drugs98

Analytical, Diagnostic and Therapeutic Techniques and Equipment15

Phenomena and Processes17

Disciplines and Occupations1

Information Science3

Health Care2

Epitopes19

Humans3

Antibody17

Molecules13

Antibodies Detected1

Peptides presented1

Histocompatibility2

Alleles7

Corresponding antigen-specific1

Affinity2

ELISPOT3

Human15

Reactivity3

Epitope12

Anti-HLA antibodies3

PBMC4

Tumor antigen3

Polymorphism1

Typing5

Recombinant1

Donor-specific1

ELISA1

Cytometric analysis4

Beta2 microglobulin3

Responses4

Immunity1

Methods1

Serology1

Molecule1

Donors1

Patients8

Cellular3

Class4

Luciferase2

Organ1

Haplotypes2

TCRs1

Lysis1

Viral2

Characterize1

Specific3

DRB11

Immune1

Lymphocytes1

Inhibition1

Protein1

Cells7

Mismatch1