Aspartic AcidAmino Acid SequenceAmino AcidsMolecular Sequence DataModels, MolecularProteinsProtein ConformationProtein Structure, SecondarySequence Homology, Amino AcidProtein Structure, TertiarySequence AlignmentSequence Analysis, ProteinAspartic Acid ProteasesDatabases, ProteinStructural Homology, ProteinBase SequenceAspartic Acid EndopeptidasesCloning, MolecularBinding SitesAmino Acid SubstitutionProtein FoldingMutagenesis, Site-DirectedSoftwareMutationStructure-Activity RelationshipAlgorithmsComputational BiologyEscherichia coliProtein BindingKineticsRecombinant ProteinsAmino Acid MotifsModels, ChemicalPeptidesCrystallography, X-RayPeptide FragmentsSubstrate SpecificityConserved SequenceComputer SimulationSequence Homology, Nucleic AcidBacterial ProteinsAsparagineInternetDNA, ComplementaryEvolution, MolecularDNAHydrogen BondingMolecular WeightPhylogenyNuclear Magnetic Resonance, BiomolecularElectrophoresis, Polyacrylamide GelCell LineThermodynamicsMembrane ProteinsAlaninePoint MutationCircular DichroismTrypsinPlasmidsAmino Acids, EssentialSequence Analysis, DNARecombinant Fusion ProteinsMolecular StructureRestriction MappingCattleCatalysisHydrogen-Ion ConcentrationHistidineUser-Computer InterfaceCysteineAmino Acid Transport SystemsChromatography, High Pressure LiquidDNA PrimersCatalytic DomainMagnetic Resonance SpectroscopyGlycineSpecies SpecificityNucleic Acid ConformationLysinePepstatinsCarrier ProteinsEndopeptidasesMutagenesisHydrophobic and Hydrophilic InteractionsProlineRNA, MessengerGenes, BacterialSerineCodonSaccharomyces cerevisiaeProtein Structure, QuaternarySequence AnalysisLigandsProtein DenaturationAmino Acids, AromaticProtein Processing, Post-TranslationalMacromolecular SubstancesLeucineOligopeptidesTemperature