• enzyme
  • These nonclassic eicosanoids are generally short-lived, being rapidly converted from epoxides to less active or inactive dihydroxy-eicosatrienoic acids (diHETrEs) by a widely distributed cellular enzyme, Soluble epoxide hydrolase (sEH), also termed Epoxide hydrolase 2. (wikipedia.org)
  • ALOX15 and ALOX15B, respectively 15(S)-HPETE → EXA4 via 15-LOX-1 EXA4 → EXC4 via LTC4 synthase aka "glutathione S-transferase II" EXC4 → EXD4 via unidentified gamma-glutamyltransferase class enzyme EXD4 → EXE4 via unidentified dipeptidase class enzyme The Arachidonic acid + O2 → 15(S)-HpETE → EXA4 → EXC4 → EXD4 → EXE4) metabolic pathway is analogous to the leukotriene-forming pathway (i.e. (wikipedia.org)
  • receptor
  • We report here that the apparent inability of isolated human polymorphonuclear leukocytes (PMNs) to efficiently transform arachidonic acid (AA) is the consequence of A 2a receptor engagement by endogenous adenosine accumulating in incubation media. (aspetjournals.org)
  • LTBR1 and LTBR2 encode for proteins with 45% amino acid identity that belong to the G protein-coupled receptor superfamily. (wikipedia.org)
  • Recently, A G protein-couple receptor of the hydroxy carboxilic acid subfamily, Niacin receptor 1, has been proposed to mediate the responses of mouse tissues to 5-oxo-ETE. (wikipedia.org)
  • The OXER1 G protein-coupled receptor resembles the hydroxy carboxilic acid subfamily of G protein-coupled receptors, which besides GPR109A, niacin receptor 1, and niacin receptor 2 may include the recently defined receptor for 12-HETE, GPR31, not only in its amino acid sequence but also in the hydroxy-carboxylic acid nature of its cognate ligands. (wikipedia.org)
  • The human gene is located on chromosome 6q27 and encodes a G-protein coupled receptor protein composed of 319 amino acids. (wikipedia.org)
  • The GPR31 receptor is most closely related in amino acid sequence to the oxoeicosanoid receptor 1, a G-protein coupled receptor encoded by the GPR170 gene. (wikipedia.org)
  • epoxidation
  • We characterized the inhibitory activity of several acetylenic and olefinic compounds on cytochrome P450 (CYP)-derived arachidonic acid ω-hydroxylation and epoxidation using rat renal cortical microsomes and recombinant CYP proteins. (aspetjournals.org)
  • Among the acetylenic compounds, 6-(2-propargyloxyphenyl)hexanoic acid (PPOH) and N-methylsulfonyl-6-(2-propargyloxyphenyl)hexanamide were found to be potent and selective inhibitors of microsomal epoxidation with IC 50 values of 9 and 13 μM, respectively. (aspetjournals.org)
  • On the other hand, 17-octadecynoic acid inhibited both ω-hydroxylation and epoxidation of arachidonic acid with IC 50 values of 7 and 5 μM, respectively. (aspetjournals.org)
  • The olefinic compounds N-methylsulfonyl-12,12-dibromododec-11-enamide (DDMS) and 12,12-dibromododec-11-enoic acid (DBDD) exhibited a high degree of selectivity inhibiting microsomal ω-hydroxylation with an IC 50 value of 2 μM, whereas the IC 50 values for epoxidation were 60 and 51 μM for DDMS and DBDD, respectively. (aspetjournals.org)
  • enzymes
  • The relative amounts of these metabolites made by specific cells and tissues depends in large part on the relative content of the appropriate enzymes. (wikipedia.org)
  • CYTOCHROME P450
  • Cytochrome P450 epoxygenases attack these double bonds to form their respective eicosatrienoic acid epoxide regioisomers (see Structural isomer, section on position isomerism (regioisomerism)) viz. (wikipedia.org)
  • epoxide
  • 5-(S)-HpETE may then be release and rapidly converted to 5(S)-HETE by ubiquitous cellular peroxidases: Arachidonic acid) + O2 → 5(S)-HpETE → 5(S)-HETE Alternatively, 5(S)-HpETE may be further metabolized to its epoxide, 5(6)-oxido-eicosatetraenoic acid viz. (wikipedia.org)
  • responses
  • Therefore, the current study was designed to more completely characterize the role of arachidonic acid metabolites in the sex-related differences in vascular responses in males versus females. (ahajournals.org)
  • These messenger ligands are any one of a range of structurally different arachidonic acid metabolites made and released by nearby cells to act as paracrine signals for coordinating responses between cells or autocrine signals for modulating their parent cells' responses. (wikipedia.org)
  • Animal studies and a limited set of human studies suggest that this family of metabolites serve as hormone-like autocrine and paracrine signalling agents that contribute to the up-regulation of acute inflammatory and allergic responses. (wikipedia.org)
  • kidney
  • The presence of a second inducible form of COX was first suggested by experiments, which showed a transient increase in the formation of PGE 2 from arachidonic acid by canine kidney cells upon stimulation with tumor promoters and carcinogens [ 5 , 6 ]. (mdpi.com)
  • membrane
  • the membrane-binding domain consists of a series of amphipathic α helices with several hydrophobic amino acids exposed to a membrane monolayer. (wikipedia.org)
  • products
  • The absence of endoperoxide-containing products derived from 10,10-difluoroarachidonic acid has been thought to indicate the importance of a C-10 carbocation in PGG2 synthesis. (wikipedia.org)
  • respectively
  • Two BLT2-like receptors, Blt2a and Blt2b, with 49% amino acid identity to each other and 34% and 29%, respectively, amino acid identities to human BLT2 have been cloned from Zebrafish embryos. (wikipedia.org)
  • receptors
  • The latter citation presents a phylogenic tree on the amino acid relateness of these two receptors as well as those from humans, monkeys, dogs, rats and mice to each other. (wikipedia.org)