• fatty acids
  • During oxygenation by 15-lipoxygenases, polyenoic fatty acids are bound at the active site in such a way that the ω-terminus of the fatty acids penetrates into the substrate binding pocket. (biochemj.org)
  • We studied the oxygenation kinetics of ω-modified fatty acids by 15-lipoxygenases and found that ω-hydroxylation strongly impaired substrate affinity (higher K m ), but only moderately altered V max . (biochemj.org)
  • These data suggest that introduction of a polar, or even charged residue, at the ω-terminus of substrate fatty acids in connection with mutation of Arg 403 may not alter substrate alignment at the active site of 15-lipoxygenases. (biochemj.org)
  • The particular feature of EDPs (and EEQs) distinguishing them from EETs is that they derive from omega-3 fatty acids and are suggested to be responsible for some of the beneficial effects attributed to omega-3 fatty acids and omega-3-rich foods such as fish oil. (wikipedia.org)
  • EETs
  • In humans, CYP2C8, CYP2C9, CYP2C19, CYP2J2, and possibly CYP2S1 isoforms appear to be the principal epoxygenases responsible for metabolizing arachidonic acid to EETs (see Epoxyeicosatrienoic acid#Production). (wikipedia.org)
  • The EDPs are commonly made by the stimulation of specific cell types by the same mechanisms which produce EETs (see Epoxyeicosatrienoic acid). (wikipedia.org)
  • Therefore
  • Therefore, these data suggest that in rabbit aorta, arachidonic acid-induced relaxations are mediated by an endothelium-dependent factor (or factors) that differs from the factor (or factors) released by acetylcholine. (ahajournals.org)
  • various
  • Isolated thoracic aortic rings were precontracted with a submaximal concentration of norepinephrine, and the effect of various agents on arachidonic acid- and acetylcholine-induced relaxations was examined. (ahajournals.org)
  • human
  • Arg 403 of the human 15-lipoxygenase has been implicated in fatty acid binding by forming a salt bridge with the carboxylate group, and thus mutation of this amino acid to an uncharged residue was supposed to favour an inverse substrate orientation. (biochemj.org)
  • respectively
  • Among the acetylenic compounds, 6-(2-propargyloxyphenyl)hexanoic acid (PPOH) and N-methylsulfonyl-6-(2-propargyloxyphenyl)hexanamide were found to be potent and selective inhibitors of microsomal epoxidation with IC 50 values of 9 and 13 μM, respectively. (aspetjournals.org)
  • On the other hand, 17-octadecynoic acid inhibited both ω-hydroxylation and epoxidation of arachidonic acid with IC 50 values of 7 and 5 μM, respectively. (aspetjournals.org)
  • The olefinic compounds N-methylsulfonyl-12,12-dibromododec-11-enamide (DDMS) and 12,12-dibromododec-11-enoic acid (DBDD) exhibited a high degree of selectivity inhibiting microsomal ω-hydroxylation with an IC 50 value of 2 μM, whereas the IC 50 values for epoxidation were 60 and 51 μM for DDMS and DBDD, respectively. (aspetjournals.org)