Reversed-phase Liquid chromatography (RP-LC) is a mode of liquid chromatography in which non-polar stationary phase and polar mobile phases are used for the separation of organic comounds. (wikipedia.org)
The vast majority of separations and analyses using High Performance Liquid Chromatography-HPLC in recent years are done using the Reversed Phase mode. (wikipedia.org)
The history and evolution of Reversed Phase stationary phases in described in details in an article by Majors, Dolan, Carr and Snyder In the 1970s, most liquid chromatography runs were performed using solid particles as the stationary phases, made of unmodified silica-gel or alumina. (wikipedia.org)
LiChrosorb® RP-8 is a reliable irregular shaped silica gel carriers with reversed phase properties. (thomassci.com)
Separations1
High efficiency separations at any pH The Hamilton PRP-C18 is a new HPLC column designed to provide high-efficiency, reversed-phase separations over an extended column life in nearly any mobile phase or pH. (thomassci.com)
There are a variety of stationary phases available for use in RPC, allowing great flexibility in the development of separation methods. (wikipedia.org)
With Reverse Phase, Normal Phase and Specialty Phases, ProteCol ensures you have the right phase for your separation needs. (thomassci.com)
SMT WCX Bulk Packings are silica-based Weak Cation eXchange packing materials developed for separation of cationic compounds. (thomassci.com)
It is well suited for the separation of neutral, acidic and weak basic compounds.This sorbent is packed into a Hibar® 125-4 HPLC column. (thomassci.com)
Besides, SHIMSEN Styra SCX is suitable for the separation of basic compounds. (shopshimadzu.com)
Retention1
At the same time hydrophobic molecules experience less affinity to the polar stationary phase, and elute through it early with not enough retention. (wikipedia.org)
Organic9
Some stationary phases are also made of hydrophobic polymeric particles, or hybridized silica-organic groups particles, for method in which mobile phases at extreme pH are used. (wikipedia.org)
The mobile phases are mixtures of water and polar organic solvents, the vast majority of which are methanol and acetonitril. (wikipedia.org)
Since the stationary phase is hydrophilic in this technique, and the mobile phase were a-polar, consisted of non-polar organic solvents such as hexane and heptane, biomolecules with hydrophilic properties in the sample had high affinity to the stationary phase, therefore they adsorb to it strongly. (wikipedia.org)
Hydrophobic molecules can be eluted from the column by decreasing the polarity of the mobile phase using an organic (non-polar) solvent, which reduces hydrophobic interactions. (wikipedia.org)
The more hydrophobic the molecule, the more strongly it will bind to the stationary phase, and the higher the concentration of organic solvent that will be required to elute the molecule. (wikipedia.org)
Hirayama, K. (1967) Handbook of Ultraviolet and Visible Absorption Spectra of Organic Compounds. (photochemcad.com)
CRC Atlas of Spectral Data and Physical Constants for Organic Compounds. (photochemcad.com)
Enrichment and purification of alkaline compounds from aqueous samples, biological fluids and organic phases. (shopshimadzu.com)
Stationary4
The longer the hydrocarbon associated with the stationary phase, the longer the sample components will be retained. (wikipedia.org)
Reversed-phase chromatography is a technique using hydrophobic hydrocarbons covalently bonded to the stationary phase particles in order to create a hydrophobic stationary phase, which has a stronger affinity for hydrophobic or less polar compounds. (wikipedia.org)
The use of a hydrophobic stationary phase is essentially the reverse of normal phase chromatography, since the polarity of the mobile and stationary phases have been inverted - hence the term reversed-phase chromatography. (wikipedia.org)
As a result, hydrophobic molecules in the polar mobile phase tend to adsorb to the hydrophobic stationary phase, and hydrophilic molecules in the mobile phase will pass through the column and are eluted first. (wikipedia.org)
Inert1
Any inert polar substance that achieves sufficient packing can be used for reversed-phase chromatography. (wikipedia.org)
In the Reversed Phase mode, the sample components are retained in the system, the more hydrophobic they are. (wikipedia.org)
Matrix1
MARV assembles and buds from the host cell plasma where MARV matrix protein (mVP40) dimers associate with anionic lipids at the plasma membrane inner leaflet and undergo a dynamic and extensive self-oligomerization into the structural matrix layer. (uci.edu)
Mobile phase2
Moreover, they were not dissolved easily in the mobile phase solvents. (wikipedia.org)
The present invention also relates to specific compounds that have been identified, using such screening methods, as useful in the treatment and/or the prevention of HCC/cirrhosis. (sumobrain.com)
High1
ODS-A is a high coverage, fully endcapped C18 phase which exhibits exceptional batch-to-batch reproducibility. (thomassci.com)
Determination1
1963) Tables of Spectrophotometric Absorption Data of Compounds Used for the Colorimetric Determination of Elements. (photochemcad.com)
Since the stationary phase is hydrophilic in this technique, and the mobile phase were a-polar, consisted of non-polar organic solvents such as hexane and heptane, biomolecules with hydrophilic properties in the sample had high affinity to the stationary phase, therefore they adsorb to it strongly. (wikipedia.org)
Reversed-phase chromatography is a technique using hydrophobic hydrocarbons covalently bonded to the stationary phase particles in order to create a hydrophobic stationary phase, which has a stronger affinity for hydrophobic or less polar compounds. (wikipedia.org)
Solvent3
Hydrophobic molecules can be eluted from the column by decreasing the polarity of the mobile phase using an organic (non-polar) solvent, which reduces hydrophobic interactions. (wikipedia.org)
The more hydrophobic the molecule, the more strongly it will bind to the stationary phase, and the higher the concentration of organic solvent that will be required to elute the molecule. (wikipedia.org)
In this study, we used anion supramolecular solvent (SUPRAS) prepared from a mixture of an anionic surfactant, sodium dodecyl sulfate (SDS), and a cationic surfactant, tetrabutylammonium bromide (TBABr), as the extraction solvent in liquid phase microextraction (LPME) of paraquat (PQ) and diquat (DQ). (mdpi.com)
Liquid2
Reversed-phase Liquid chromatography (RP-LC) is a mode of liquid chromatography in which non-polar stationary phase and polar mobile phases are used for the separation of organic comounds. (wikipedia.org)
The history and evolution of Reversed Phase stationary phases in described in details in an article by Majors, Dolan, Carr and Snyder In the 1970s, most liquid chromatography runs were performed using solid particles as the stationary phases, made of unmodified silica-gel or alumina. (wikipedia.org)