Aptamers. Medical search. Frequent questions

We also developed an additional lateral flow assay for IgE, in which the analyte is sandwiched between immobilized anti-IgE antibodies and aptamer-bearing reporter phage modified with HRP. (nih.gov)
Here we have developed an aptamer-based fluorescence polarization assay for exosome quantification, which is a separation-free, amplification-free and sensitive approach enabling direct quantification of exosomes in human plasma. (rsc.org)
and a new aptamer-based dye-displacement assay that sensitively screens for fentanyl and its analogs with naked eye. (ojp.gov)
An integrated colorimetric assay using this aptamer can detect five different variants, while other non-universal aptamers could only effectively detect one or two. (chemistryviews.org)
The overall objective of this Small Business Innovation Research (SBIR) program is to develop an aptamer-based assay for the early detection of F. psychrophilum in a common sensor platform, such as ELISA or real-time PCR. (usda.gov)
A successful Phase I will yield an aptamer-based assay which should detect F. Psychrophilum at much lower levels than previously achievable. (usda.gov)
A higher specificity and selectivity aptamer based assay will provide US fish farmers and fish health laboratories with the ability to perform earlier detection of the onset of CWD or allow quantitative screening of broodstock in order to implement strategies that would limit vertical transmission to progeny. (usda.gov)
The aptamers were tested in a platelet functionality assay (PFA-100) which measures time until platelet plug formation in vitro . (duke.edu)
A simple displacement aptamer assay on resistive pulse sensor for small molecule detection by: Rhushabh Maugi, et al. (lboro.ac.uk)

These two aptamers have high affinity and good specificity and have been widely studied and used for the development of aptamer-based therapeutics and diagnostics. (wikipedia.org)
This document reports on a research project with the overall goal of developing aptamer-based assays that can perform rapid, on-site detection of opioid families in seized substances with high sensitivity and specificity. (ojp.gov)
The product of a successful Phase I program will be the development of aptamer with high selectivity and specificity for F. Psychrophilum. (usda.gov)
Confirm the aptamer selectivity and specificity in vitro. (usda.gov)
The AptoPrep™ cell isolation kit products from AMSBIO combine the advantage of the specificity of aptamer to target cell with the convenience of isolation/elution of a particular cell from complex cell samples. (labwrench.com)
AMSBIO has also introduced a new range of dye-conjugated monoclonal aptamers specifically designed to monitor protein expression by flow cytometry with high specificity by minimising the background caused by non-specific binding. (labwrench.com)
Aptamers are single-stranded RNA or DNA oligonucleotides that fold up into a distinctive 3D structure capable of binding with high affinity and specificity to small molecules up to entire organisms, with nanomolar range dissociation constants. (encyclopedia.pub)
Many approaches have been introduced to promote the stability of aptamers without altering their binding affinity and specificity against their targets. (encyclopedia.pub)
Aptamer target-binding specificity and affinity are obtained using a Darwinian evolution screening technology called systematic evolution of ligands by exponential enrichment (SELEX). (encyclopedia.pub)
Aptamers serve as alternative recognition elements instead of antibodies in LFB, offering better specificity and stability. (cdc.gov)

Anti-thrombin aptamers are G-quadruplex-bearing oligonucleotides, which recognizes the exosites of human thrombin. (wikipedia.org)
Aptamers 2016 will be co-hosted with our 2nd symposium on antisense and therapeutic oligonucleotides, Oligo 2016 , on 6th April 2016. (libpubmedia.co.uk)
Infoscitex will develop high affinity aptamers (synthetic DNA oligonucleotides) capable extremely high sensitivity detection of the pathogen (bacteria) or material deriving from the pathogen. (usda.gov)
Aptamers are oligonucleotides or peptides that bind to a specific target molecule. (labwrench.com)
Aptamers are short single-stranded RNA or DNA oligonucleotides capable of folding into complex 3D structures, enabling them to bind to a large variety of targets ranging from small ions to an entire organism. (encyclopedia.pub)
Nucleic acid-based aptamers were first described in 1990, where the first aptamers were in vitro selected using a random library of single-stranded oligonucleotides sequences [ 1 ] by a selection procedure known as systematic evolution of ligands by exponential enrichment (SELEX). (encyclopedia.pub)
Chemical modifications of aptamer oligonucleotides are needed mainly to enhance their resistance to nuclease degradation and lowering their renal filtration. (encyclopedia.pub)

Pure Biologics has entered into a cooperation agreement with the Dutch company Relitech Besloten Vennootschap to develop an aptamer-based medical device to remove toxins from the blood of patients with chronic kidney disease and the risk of developing cardiovascular disease and undergoing hemodialysis, the company said. (biznespolska.pl)

Aptamer Group, the developer of novel Optimer binders to enable innovation in the life sciences industry, today announces the appointment of Derek Smith as Interim Chief Commercial Officer, effective as of 1 January 2023. (manufacturingchemist.com)
Chemical Modifications of Aptamers" Encyclopedia , https://encyclopedia.pub/entry/16686 (accessed December 05, 2023). (encyclopedia.pub)

The developed aptamer will be used in place of antibodies to detect with greater sensitivity the pathogen and quantify its concentration by quantitative real-time polymerase chain reaction (qRT-PCR). (usda.gov)
The company conducts research using its own technological platforms for the selection of active particles - antibodies and aptamers. (biznespolska.pl)
Here, we report a novel PDGFRβ-specific antagonist represented by a nuclease-resistant RNA-aptamer, named Gint4.T. Aptamers, thanks to their unique characteristics (low size, good target affinity, no immunogenicity, high stability), represent a new class of molecules with a great potential to rival monoclonal antibodies in both therapy and diagnosis. (unina.it)
Aptamers are useful in biotechnological and therapeutic applications as they offer molecular recognition properties that rival that of the commonly used biomolecule, antibodies. (labwrench.com)
In addition to their discriminate recognition, aptamers offer advantages over antibodies as they can be engineered completely in a test tube, are readily produced by chemical synthesis, possess desirable storage properties, and elicit little or no immunogenicity in therapeutic applications. (labwrench.com)

We introduce the modification of bacteriophage particles with aptamers for use as bioanalytical reporters, and demonstrate the use of these particles in ultrasensitive lateral flow assays. (nih.gov)
Alternative ELISA based assays utilizing such aptamers will also be developed. (usda.gov)

Aptamers are short, single-stranded DNA or RNA molecules that can selectively bind to a specific target. (chemistryviews.org)
Aptamers, on the other hand, are single-stranded. (scitechdaily.com)
Aptamers are single-stranded pieces of DNA that bind to target proteins and are able to modulate signaling cascades during cell differentiation when a stem cell commits to a specific functional role or phenotype. (shinshu-u.ac.jp)

M13 phage displaying an in vivo biotinylatable peptide (AviTag) genetically fused to the phage tail protein pIII were used as reporter particle scaffolds, with biotinylated aptamers attached via avidin-biotin linkages, and horseradish peroxidase (HRP) reporter enzymes covalently attached to the pVIII coat protein. (nih.gov)
The team used a pool of aptamers that bind to the wild-type spike protein and carried out aptamer selection experiments using five different spike proteins. (chemistryviews.org)
We demonstrate a strategy for label-free protein detection through monitoring the Surface Enhanced Raman Spectrum of an aptamer probe attached to a gold nanoshell . (rsc.org)
In this work, in silico selection of DNA-aptamers to the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein was performed using molecular modeling methods. (jyu.fi)
Based on molecular docking results, the only one aptamer (Apt16) was selected from the library as a starting aptamer to the RBD protein. (jyu.fi)
Binding of the each modeled aptamer to the RBD was studied in terms of interactions between residues in protein and nucleotides in the aptamers. (jyu.fi)
We used such a library to isolate aptamers that can attach to the spike protein of SARS coronavirus 2," explains Prof. Dr. Günter Mayer of the LIMES Institute (the acronym stands for "Life and Medical Sciences") at the University of Bonn. (scitechdaily.com)
The now isolated aptamer with the abbreviation SP6 also binds to the spike protein, but at a different site. (scitechdaily.com)
will preferentially be DNA aptamers developed ad hoc against either whole parasite cells or specific known protein markers of the pathogens. (ibecbarcelona.eu)
Aptamers offer several advantages compared to protein or small-molecule drugs, most notably their ease of production, low risk of inducing an immune reaction in humans, and amenability to chemical modifications that enhance their drug-like properties, including improved stability and residence time in the bloodstream. (bccresearch.com)
By attaching itself to the virus surface, the RNA aptamer prevents the Spike protein from serving as a key that allows the virus to enter a cell. (bioquicknews.com)

We coupled aptamers, a new type of biosensor comprising a single strand of synthetic DNA with customizable sequences, on the microelectronic fibers. (tohoku.ac.jp)
Ye, Z-Z. Application of biosensor surface immobilization methods for aptamer. (eurekaselect.com)
In this work, we developed a fluorescent aptamer-based lateral flow biosensor (apta-LFB) integrated with fluorophore-quencher nano-pairs and a smartphone spectrum reader to accomplish triple-target detection of chlorpyrifos, diazinon, and malathion. (cdc.gov)

Aptamers were tagged with ferrocene as an electrochemical readout for target molecule and aptamer binding. (tohoku.ac.jp)
An aptamer is a piece of DNA or RNA that folds into a 3D structure that can recognize a specific target molecule of interest. (bioquicknews.com)
Aptamers are DNA or RNA molecules with special conformational features, able to efficiently bind to a target molecule. (interesjournals.org)
The length of an aptamer is not a function of its size but it's ability to bind to a target molecule. (neoaptamers.com)

Derek joined the company in 2018 as Director of Global Sales and has since become a vital part of the Aptamer team, overseeing the development and implementation of the company's global sales strategy and securing contracts across all three business divisions, including with a number of major pharmaceutical companies. (manufacturingchemist.com)
Dr Arron Tolley, Chief Executive Officer, commented: "Since joining Aptamer in 2018, Derek's business acumen and commitment to his role have contributed to an impressive increase in our sales and revenue. (manufacturingchemist.com)
However, the aptamer delivery category, which was valued at just $218 million in 2013, is expected to jump to nearly $2.2 billion in 2018. (bccresearch.com)

Oligonucleotide aptamers are well known for their stability and flexibility in conjugating various inflammatory molecules. (iasp-pain.org)
The aptamer is bound to the pore by hybridization to an oligonucleotide that is attached covalently through a disulfide bond to a single cysteine residue near a mouth of the pore. (huji.ac.il)
The first oligonucleotide aptamer was isolated to bind with small molecules [ 2 ] . (encyclopedia.pub)

They discovered a unique aptamer named MSA52. (chemistryviews.org)
To gain further insights on the structure of this unique aptamer, we have determined several high-resolution crystal structures of 93del and its variants. (ntu.edu.sg)

During year 2, the fellow will develop DNA aptamers against the selected parasite cells and/or recombinant proteins from the pathogens and will explore their capacity to block their growth in in vitro cultures. (ibecbarcelona.eu)
In addition, this in silico workflow would be potentially applied in the more extensive in vitro development and application of RNA -templated ssDNA aptamers targeting glycans . (bvsalud.org)

The first anti-thrombin aptamer, TBA, was generated through via SELEX (Systematic Evolution of Ligands by Exponential Enrichment) technology in 1992 by L.C. Bock, J.J. Toole and colleagues. (wikipedia.org)
A second thrombin-binding aptamer, HD22, recognizes thrombin exosite II and was discovered in 1997 by NeXstar (now Gilead Sciences). (wikipedia.org)
It interacts with the exosite I of human alpha-thrombin, which is the binding site of fibrinogen, so this aptamer acts as an anti-coagulant agent inhibiting the activation of fibrinogen as well as platelet aggregation. (wikipedia.org)
Some groups use this property to develop aptamer-based thrombin sensors. (wikipedia.org)
This aptamer works by binding and blocking a major molecule involved in the body's blood-clotting reaction, called thrombin. (merriam-webster.com)
We show that the binding of thrombin to a 15-mer DNA aptamer, which forms a cation-stabilized quadruplex, alters the ionic current through the pore. (huji.ac.il)

Like a key fits into a specific lock and opens it, an aptamer can bind to a specific molecule and interact with it in a selective manner. (tohoku.ac.jp)
The newly developed molecule belongs to a class of compounds known as RNA aptamers and it is based on the same type of building blocks that are used for mRNA vaccines. (bioquicknews.com)

The symposium will address therapeutic, diagnostic, analytical as well as basic research applications of aptamers and invites proposals for podium (now closed! (libpubmedia.co.uk)
This review summarizes the progress made in the use of aptamers as therapeutic agents, paying special attention to those examples that have concluded in pharmacological formulations currently included in preclinical and clinical trials. (interesjournals.org)

Ribomic paid Archemix $6 million for rights to develop aptamers against multiple targets. (genengnews.com)
In the study, the defined aptamer sequences bound to specific molecules with a high affinity, inducing a reversible three-dimensional conformational change from a relatively simple chain structure into a complex structure wrapping around targets. (tohoku.ac.jp)
The proposed computational approach to selection and refinement of aptamers is universal and can be used for wide range of molecular ligands and targets. (jyu.fi)
Targets on which aptamer selection is conducted against are diverse, ranging from ions to whole living cells. (encyclopedia.pub)

Aptamers are synthetic nucleic acid-based affinity ligands that are isolated based on their ability to bind specifically and with high affinity to a given target. (2bscientific.com)
A team of researchers from Shinshu University, the National Institute of Advanced Industrial Science and Technology, and the University of Shizuoka in Japan set out to address this question by leveraging nucleic acid aptamers. (shinshu-u.ac.jp)
Nucleic acid aptamer binding. (checkorphan.org)
Moreover, introducing chemical modifications into nucleic acid libraries increases the interaction capabilities of aptamers and thereby their target spectrum [ 12 ] . (encyclopedia.pub)

Ribonucleic acid ligands, or aptamers, are a class of drugs that are ideal for blocking GPVI. (duke.edu)

For Apt16/RBD complex, molecular dynamic and quantum chemical calculations revealed the pairs of nucleotides and amino acids whose contribution to the binding between aptamer and RBD is the lar gest. (jyu.fi)
We use 80 nucleotide sequences in our aptamer selection process, with a random region of 40 nucleotides. (neoaptamers.com)
The extra nucleotides on a longer aptamer are not involved in the binding event and can reduce both the rate of complex formation by getting in the way, and the rate of complexes coming apart by reducing the stability of the aptamer structure. (neoaptamers.com)
Modified aptamers may show improved chemical diversity relative to aptamers composed entirely of natural DNA or RNA nucleotides and expand their applications in diagnostics, therapeutics, and nanotechnology [ 1 ] . (encyclopedia.pub)

Selection and Validation of High-Affinity ssDNA Aptamers Targeting Paromomycin. (bvsalud.org)
In this work , the model of interactions between glycans and ssDNA aptamers synthesized based on the sequence of rRNA genes was developed. (bvsalud.org)

Aptamer-based fluorescent sensors specifically detect the antimalarial drugs piperaquine and mefloquine from patient samples and crushed tablets. (ox.ac.uk)
The design and characterization of multifunctional aptamer nanopore sensors by: Laura J. Mayne, et al. (lboro.ac.uk)

Based upon fluorescent dye-conjugated aptamers - AptoCyto™ flow cytometry kits are stable for at least several months at room temperature. (labwrench.com)

Aptamers to components of the replication complex are therefore useful molecular tools for studying viral replication and also have potential as diagnostic molecules in the future. (whiterose.ac.uk)

Due to its exceptional stability and unique topology, 93del has been used in many different studies involving DNA G-quadruplexes, such as DNA aptamer and multimer design, as well as DNA fluorescence research. (ntu.edu.sg)

Incorporating bifunctional aptamer labeled with FITC and biotin the kits allow you to perform cell isolation and flow cytometry analyses simultaneously, further improving your productivity. (labwrench.com)

aptamer interactions. (huji.ac.il)

Using the SELEX methodology, the inventors generated multiple high affinity aptamers against GPVI from a pool of modified RNA sequences. (duke.edu)

The authors of this report describe research that resulted in the development of an electrochemical aptamer-based (E-AB) sensor as a presumptive testing tool that can be easily used by law enforcement agents, first responders, or forensic personnel to identify opioid families in seized substances. (ojp.gov)

The SELEX methodology is quite similar for both DNA and RNA aptamers. (encyclopedia.pub)

Mild and unique cell releasing technology allows you to isolate bead- and aptamer-free cells in as little as 1 hour. (labwrench.com)

View a selection of Aptamers 2016 photos on the INSOAP website . (libpubmedia.co.uk)

93del is a 16-nucleotide G-quadruplex-forming aptamer which can inhibit the activity of the HIV-1 integrase enzyme at nanomolar concentration. (ntu.edu.sg)

The sensitivity and selectivity levels of this aptamer will be characterized to demonstrate its advantages over current antibody tests. (usda.gov)

Reference: "A SARS‐CoV‐2 Spike Binding DNA Aptamer that Inhibits Pseudovirus Infection by an RBD‐Independent Mechanism" by Anton Schmitz, Anna Weber, Mehtap Bayin, Stefan Breuers, Volkmar Fieberg, Michael Famulok and Günter Mayer, 8 March 2021, Angewandte Chemie International . (scitechdaily.com)

A new computational approach to aptamer in silico selection is based on a cycle of simulations, including the stages of molecular modeling, molecular docking, molecular dynamic simulations, and quantum chemical calculations. (jyu.fi)
As a result, four aptamers (Apt16, Apt25, Apt27, Apt31) to the RBD were designed in silico without any preliminary experimental data. (jyu.fi)

Moreover, Gint4.T aptamer drastically inhibits cell migration and proliferation, induces differentiation, and blocks tumor growth in vivo. (unina.it)
As a result, the combination of Gint4.T and an epidermal growth factor receptor-targeted aptamer is better at slowing tumor growth than either single aptamer alone. (unina.it)

Aptamers are increasingly being used across the life sciences in research, diagnostics and therapeutics. (aptamergroup.com)

an anti-nucleolin DNA aptamer, iSN04, can determine a cell's lineage during differentiation. (shinshu-u.ac.jp)
The team, led by Associate Professor Tomohide Takaya from the Department of Agricultural and Life Sciences at Shinshu University, recently discovered that an anti-nucleolin aptamer, myogenetic oligodeoxynucleotide iSN04, induced myocardial differentiation in embryonic stem cells (ESCs). (shinshu-u.ac.jp)
We had previously found that iSN04 promoted myogenic precursor cells (myoblasts) to differentiate into skeletal muscle cells and had hypothesized that the aptamer also enhanced differentiation of pluripotent stem cells. (shinshu-u.ac.jp)

Yingfu Li, McMaster University, Hamilton, Canada, and colleagues have developed a universal DNA aptamer that can help to detect all major VoCs. (chemistryviews.org)

These defined aptamers sense neurochemicals with high sensitivity and high selectivity. (tohoku.ac.jp)

Our simulation-based approach revealed that paromomycin as a representative example of glycans is preferred to bind base-restricted stem structures of aptamers because they are more critical in stabilizing the flexible structures of glycans . (bvsalud.org)

This makes them interesting for research into active ingredients, especially since it is now very easy to produce huge libraries of different aptamers. (scitechdaily.com)
Mayer also heads the Center of Aptamer Research and Development (CARD) at the University of Bonn. (scitechdaily.com)
Sobiepanek, A. Aptamer-based advances in skin cancer research. (eurekaselect.com)

In addition, Gint4.T aptamer prevents PDGFRβ heterodimerization with and resultant transactivation of epidermal growth factor receptor. (unina.it)

Merriam-Webster.com Medical Dictionary , Merriam-Webster, https://www.merriam-webster.com/medical/aptamer. (merriam-webster.com)

Here, a new type of stochastic sensor based on an αHL pore modified with an aptamer is described. (huji.ac.il)

The procedure of 1) aptamer structure modeling/modification, 2) molecular docking, 3) molecular dynamic simulations, 4) quantum chemical calculations was performed sev-eral times. (jyu.fi)

Aptamers need to be stabilized for in vivo use against nuclease degradation, and their small size makes them susceptible to renal filtration. (encyclopedia.pub)

Yüce, M. Aptamer-based emerging tools for viral biomarker detection: A focus on SARS-CoV-2. (eurekaselect.com)

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