Approaches mutagenesis insertional. Medical search. Frequent questions

Moreover, since more than half of Plasmodium genes are required for normal asexual blood-stage reproduction, and cannot be targeted using knockout methods, we discuss how CRISPR/Cas9 could be used to scale up conditional gene knockdown approaches to systematically assign function to essential genes. (portlandpress.com)
To this end they are applying a newly developed insertional mutagenesis approach which, in combination with tail-PCR procedures, allows them to rapidly clone genes mutated by the insertion of a selectable marker gene by transformation. (umn.edu)
While the selective inactivation of Ophiostoma genes by insertional mutagenesis remains a challenge, an alternative approach based on RNA interference is now available for down-regulating the expression of targeted genes. (sisef.it)
Whole-genome transposon-mutagenesis (TM) followed by identification of insertion sites is one of the most practical and frequently used experimental approaches to screen for essential bacterial genes [ 6 - 8 ]. (biomedcentral.com)
Recently, several groups have used transposon-based approaches to deliver genes to a variety of cells. (tamu.edu)
Theoretically, this approach decreases the risk of insertional mutagenesis compared with repeated, separate integrations of multiple genes and associated promoters into the T-cell genome. (ubigene.us)
Multiple mutations affecting embryonic development have been induced by chemical and insertional mutagenesis resulting in the identification of genes with important roles in development in Drosophila [ 23 - 25 ]. (biomedcentral.com)
Using this approach we successfully identify genes involved in paclitaxel resistance in a variety of cancer cell lines, including the multidrug transporter ABCB1, a previously identified major paclitaxel resistance gene. (biomedcentral.com)
Analysis of co-occurring transposons integration sites in single cell clone allows for the identification of genes that might act cooperatively to produce drug resistance a level of information not accessible using RNAi or ORF expression screening approaches. (biomedcentral.com)
Another approach involves delivering genes that regulate myocardial contractility, aiming to improve the heart's pumping ability. (aliyuncs.com)

We therefore introduce the reader to CRISPR-based screens in the related apicomplexan Toxoplasma gondii and discuss how these approaches could be adapted to develop CRISPR/Cas9 based genome-scale genetic screens in malaria parasites. (portlandpress.com)
A large-scale, gene-driven mutagenesis approach for the functional analysis of the mouse genome. (nih.gov)
Lentiviruses, a subspecies of retroviruses, integrate their cargo into the host's genome, assuring long-term expression of the delivered gene, but carrying the risk of random insertional mutagenesis. (encyclopedia.pub)
Mutagenesis with mobile genetic elements that insert into the genome offers a great scope for screening as these provide readily detected tags to identify insertion sites, and can potentially either activate or disrupt gene expression. (biomedcentral.com)
The high fecundity of the frog combined with the ability to remobilize transposon transgenes integrated into frog genome will allow large-scale insertional mutagenesis screens to be performed in laboratories with modest husbandry capacities. (biomedcentral.com)
We constructed a map of unbalanced SVs (that is, copy number variants) based on whole genome DNA sequencing data from 185 human genomes, integrating evidence from complementary SV discovery approaches with extensive experimental validations. (nih.gov)
It also creates the potential for off-target genome modification leading to aberrant gene expression, chromosomal translocation induction of malignancies, risk for insertional mutagenesis, associated risk of tumorgenicity, and the possibility of immune response. (propharmagroup.com)
Comprehensive Functional Analysis of the Enterococcus faecalis Core Genome Using an Ordered, Sequence-Defined Collection of Insertional Mutations in Strain OG1RF. (cdc.gov)

Finally, retroviruses are prone to insertional mutagenesis and random integration creates variation between iPSC clones. (addgene.org)
tax causes malignant transformation not through integration and insertional mutagenesis, as many retroviruses do, but by inhibition of DNA repair (base excision pathway) and trans-activating disruption of cellular growth control mechanisms ( 2 ). (cdc.gov)

The contributors describe strategies for producing genetic models, including transgenic germline models, gene knockouts and knockins, and conditional and inducible systems, as well as models derived using transposon-based insertional mutagenesis, RNA interference, viral-mediated gene delivery, and chemical carcinogens. (cshlpress.com)

These approaches should help overcome some of the drawbacks of viral vectors used in the majority (∼ 60%) of clinical trials for cancer gene therapy, including potential malignant transformation due to insertional mutagenesis with retroviral delivery and pre-existing immune responses to adenoviral proteins. (ox.ac.uk)

First by inducing chromosome instability and second by creating insertional mutagenesis of defective retroviral elements such as IAPs. (uni-muenchen.de)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells. (nih.gov)

Investigations of the possible cause of leukemia revealed insertional mutagenesis of the therapeutic gene into a proto-oncogene locus [ 10 ] . (encyclopedia.pub)
One particularly influential approach in this regard is the use of adenoviral vectors, which hold great potential for unlocking the therapeutic benefits of gene therapy. (aliyuncs.com)
Adoptive immunotherapy is an established and evolving therapeutic approach. (ashpublications.org)

Molecular and omic approaches, however, represent technological advances that should allow rapid developments in the genetic analysis of elms and bark beetle vectors of the disease. (sisef.it)
Viral vectors also have the largest genomic footprint of iPSC generating methods due to the risk of insertional mutagenesis. (addgene.org)
These include immunity against rAAV vectors, limited transgene packaging capacity, sub-optimal tissue transduction, potential risks of insertional mutagenesis and vector shedding. (bvsalud.org)
With further progress in this field, gene therapy using adenoviral vectors may become a transformative approach in improving patient outcomes and quality of life for individuals like John. (aliyuncs.com)
Transposon vectors can be used in the frog for transgenesis and for insertional mutagenesis where enhancer trap and gene trap constructs are used to identify genomic loci involved in developmental processes. (biomedcentral.com)

Knowledge of the genetic networks controlling embryogenesis has been obtained principally by mutagenesis screens in model species. (biomedcentral.com)
The rapid accumulation of genetic resources for this animal and the success of pilot mutagenesis screens have helped propel this model system forward. (biomedcentral.com)

After nearly 100 years of documented Dutch elm disease (DED) presence, it seems only fitting to revisit this pathosystem from a genetic standpoint encompassing both traditional Mendelian genetics and the most recent developments in omic-based approaches. (sisef.it)

However, limited cargo capacity, host immune response, and the risk of insertional mutagenesis are limiting factors and of concern. (tamu.edu)

Finally, we explored the off-target genomic toxicity of our genomic engineering approach. (biomedcentral.com)
Understanding the biology of helminths using genomic and proteomic approaches could contribute to advances in understanding host-helminth interactions and lead to new vaccines, drugs and diagnostics. (biomedcentral.com)

A mutant library was created with insertional mutagenesis via electroporation in order to transform a BASTA resistance gene into conidial nuclei. (uni-muenchen.de)
Here we describe a gain-of-function forward genetic approach for identifying mechanisms of resistance. (biomedcentral.com)
This cost-effective approach can be readily applied to different cell lines, to identify canonical or context specific resistance mechanisms. (biomedcentral.com)
A complementary approach is to identify resistance pathways experimentally using in vitro culture or animal model systems. (biomedcentral.com)
Resistance of high fitness hepatitis C virus to lethal mutagenesis. (cdc.gov)

The identification of several mutants with altered clock phenotypes has on one hand confirmed the general approach of this study and on the other proved that the greater sensitivity for the temperature screen can bee used to detect mutant phenotypes. (uni-muenchen.de)

These integration systems are ideally suited to transgenesis and insertional mutagenesis strategies in the frog. (biomedcentral.com)

During the last decades, the development of ever more powerful genetic, molecular and omic approaches has provided plant pathologists with a wide array of experimental tools for elucidating the intricacies of plant-pathogen interactions and proposing new control strategies. (sisef.it)

Although several gene delivery approaches have been tested in preclinical animal models, including viral and nonviral approaches, adeno-associated virus (AAV) vector-based gene transfer remains the most popular gene delivery platform for the liver, with a few trials entering phase III. (abdominalkey.com)
Helper-independent piggyBac plasmids for gene delivery approaches: strategies for avoiding potential genotoxic effects. (tamu.edu)

The results presented herein demonstrate that our helper-independent plasmids represent an important step in the development of safe and efficient gene delivery methods that should prove valuable in gene therapy and transgenic approaches. (tamu.edu)
Transcriptomic analyses provide an efficient approach to explore the temporal gene expression profiles in embryos and to search for the developmental regulators. (biomedcentral.com)
The question he asked at that time was simple: how to be able to monitor the transposition of these mobile elements in a mammal where genetic approaches to detect transposition were both poorly efficient and time consuming. (biomedcentral.com)

In this chapter, we will discuss some of the key approaches to GT for liver-based metabolic diseases (LBMDs), with a focus on the advances of gene addition approaches and AAV-based GT strategies, and will present some of the main achievements, and emerging issues, of the field of in vivo gene transfer. (abdominalkey.com)
Bone marrow stem cells, including the pluripotent hematopoietic stem cells (HSCs) and bone mesenchymal stem cells (BMSCs), are being considered as potential targets for cell and gene therapy-based approaches against a variety of different diseases. (hindawi.com)

This approach was used in 2012 in clinical trials to try to treat patients with Familial Adenomatous Polyposis. (wikipedia.org)
In particular, viral based gene therapy approaches have proved dangerous in past clinical trials. (wikipedia.org)
We provide an in-depth analysis of factors determining AAV seroprevalence and examine clinical approaches to managing anti-AAV NAbs pre- and post-vector administration. (bvsalud.org)
Non-viral site directed CAR integration can be accomplished using CRISPR/Cas9 and double-stranded DNA (dsDNA) or single-stranded DNA (ssDNA) via homology-directed repair (HDR), however yields with this approach have been limiting for clinical application (dsDNA) or access to large yields sufficient to meet the manufacturing demands outside early phase clinical trials is limited (ssDNA). (biomedcentral.com)

Forward genetic approaches using mobile genetic elements provide a powerful alternative method for gene discovery that can overcome many of the limitations of reverse genetic approaches. (biomedcentral.com)

In an effort to study the effect of changed methylation levels in vivo and its effect on disease progression, we developed a genetic approach to study the effect of hypomethylation during embryogenesis and adulthood. (uni-muenchen.de)

Phylogenetic approach to recover integration dates of latent HIV sequences within-host. (cdc.gov)

Over the past few decades, multiple approaches have been used to explore the mechanisms of liver carcinogenesis. (biomedcentral.com)

In order to address the shortcomings related to current treatment modalities, many groups have diverted their attention to utilizing tissue engineering and regenerative medicine approaches. (biomedcentral.com)
The purpose of this review is to highlight current bioengineering approaches for salivary gland tissue engineering and the adult stem cell sources used for this purpose. (biomedcentral.com)
This article is intended to provide a review of the most current approaches to utilizing stem cells and bioengineering principles for the purpose of salivary tissue regeneration. (biomedcentral.com)

Perhaps more importantly, as reverse genetic approaches, they are biased toward previously characterized genetic elements. (biomedcentral.com)

The piggyBac (pB) transposase in particular has been shown to be well suited for cell transfection and gene therapy approaches because of its flexibility for molecular modification, large cargo capacity, and high transposition activity. (tamu.edu)
This approach uses a modified piggyBac transposon to generate libraries of mutagenized cells, each containing transposon insertions that randomly activate nearby gene expression. (biomedcentral.com)

Approaches to characterize tumor development, progression, and metastasis in these models using state-of-the-art imaging, histopathological, surgical, and other techniques are also included. (cshlpress.com)
Although current treatments, such as traditional pharmacological approaches, are often effective for limited periods, these therapies have no practical significance for the progression of pain and can even induce tolerance and unacceptable systemic side effects. (hindawi.com)

Use of a bacterial vector to obtain shRNA expression in cells is a relatively recent approach. (wikipedia.org)
Although exciting results have been achieved by means of somatic cell nuclear transfer, cell fusion, and culture-induced reprogramming [ 1 ], these procedures are technically demanding and inefficient and therefore unlikely to become a common approach for producing patient-specific pluripotent cells. (biomedcentral.com)

We examined numerous whole and partial gene deletions with a genotyping approach and observed a depletion of gene disruptions amongst high frequency deletions. (nih.gov)

My approach was initially that of a physicist, and I managed to identify the rapid allosteric transitions that proved to be associated with the binding of acetylcholine to its receptor, and resulting in the opening of the ion channel. (biomedcentral.com)

In recent years, these powerful genetic tools have been used in higher vertebrates for transgenesis, insertional mutagenesis, and gene therapy applications. (biomedcentral.com)

In this review, we outline current reprogramming approaches developed to improve the safety and efficiency of the method and highlight its critical aspects that may have an impact on the quality of the iPSCs. (biomedcentral.com)

Although a cautious approach may have been prudent initially, we argue that some regulatory requirements can now be modified to reduce costs and uncertainty without compromising safety. (nature.com)

To filter errors resulting from these intrinsic biases in the "negative" TM approach, a statistical framework has recently been developed and tested in P. aeuginosa PAO1 and Francisella tularensis novicida [ 7 ] TM datasets. (biomedcentral.com)

Anatomy10

Organisms14

Diseases1

Chemicals and Drugs90

Analytical, Diagnostic and Therapeutic Techniques and Equipment38

Phenomena and Processes108

Technology, Industry, Agriculture1

Information Science4

Health Care3

Genes10

Genome8

Retroviruses2

Transposon-based1

Malignant transformation1

Retroviral1

Mutations1

Therapeutic3

Vectors5

Screens2

Genetics1

Risk1

Genomic2

Resistance5

Phenotypes1

Ideally1

Experimental1

Gene delivery approaches2

Efficient3

Diseases2

Clinical4

Overcome1

Embryogenesis1

Integration1

Mechanisms1

Tissue3

Genetic approaches1

PiggyBac2

Progression2

Cells2

High1

Identify1

Applications1

Review1

Initially1

Framework1