• gene
  • Overexpression of c-myc gene into cultured fibroblast cells has been reported to elicit massive apoptosis upon serum deprivation, in diploid rat fibroblasts, serum deprivation induced apoptosis following withdrawal of serum, which was antagonized by the addition of low doses of ET-1. (nii.ac.jp)
  • The apoptosis observed in the diploid fibroblasts has been demonstrated to be c-myc-dependent, since disruption of one endogenous c-myc gene copy using targeted homologous recombination resulted in marked decrease in serum deprivation-induced apoptosis. (nii.ac.jp)
  • Publications] Shichiri M.et al: 'Biphasic regulation of the preproendothelin-1 gene by c-Myc' Endocrinology. (nii.ac.jp)
  • Publications] Iwashita M.et al: 'Transfection of inducible n/tric oxide synthase gene causes apoptosis in vascular smooth muscle cells' Circulation. (nii.ac.jp)
  • Western blot analysis and RT-PCR revealed that mollugin suppressed activation of NF- κ B and NF- κ B-dependent gene products involved in antiapoptosis (Bcl-2 and Bcl-xl), invasion (MMP-9 and ICAM-1), and angiogenesis (FGF-2 and VEGF). (hindawi.com)
  • MAPKs are involved in directing cellular responses to a diverse array os stimuli, such as mitogens, osmotic stress, heat shock and proinflammatory cytokines regulating cell functions including proliferation, gene expression, differentiation, mitosis, cell survival or apoptosis. (wikipedia.org)
  • Mutations on the gene that encodes cIAP1 are related to hemorrhage and vascular regression because of the defects it represents on the endothelial cell survival and the modification of apoptosis. (wikipedia.org)
  • To determine the signaling pathways modulated by the S100B/RAGE interaction contributing to myocyte apoptosis, rat neonatal cardiac myocyte cultures transfected with a full-length cDNA of the RAGE gene, a dominant-negative cytoplasmic deletion mutant of RAGE, or vector control were treated with nanomolar (1-1000) concentrations of recombinant bovine brain S100B for 48 hrs. (ahajournals.org)
  • PEDF induces PPAR-gamma expression which in turn induces p53, a tumor suppressor gene involved in cell cycle regulation and apoptosis. (wikipedia.org)
  • This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. (wikipedia.org)
  • This gene and MAPK8 are also known as c-Jun N-terminal kinases. (wikipedia.org)
  • Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. (wikipedia.org)
  • Phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing gamma polypeptide is an enzyme that in humans is encoded by the PIK3C2G gene. (wikipedia.org)
  • Like apoptosis and other types of programmed cell death, the cell is involved in causing its own death, and gene expression is required. (wikipedia.org)
  • inhibitor
  • Here, we investigated the effect of OTA in the absence or presence of the ERK1/2 inhibitor U0126 [1,4-diamino-2,3-dicyano-1,4bis(2-aminophenylthio)-butadiene] to test whether OTA then will exert increased toxicity. (aspetjournals.org)
  • Cyt A inhibited ICAM-1 expression by TNF-α and IL-1α at similar concentrations, but was found to be inhibited by TAPI-2, an inhibitor to the TNF-α-converting enzyme (TACE). (wikipedia.org)
  • pathways
  • The BCR is a key signaling molecule that triggers pathways that can induce B-cell proliferation, survival, differentiation, anergy, and apoptosis (for a review, see Niiro and Clark 12 ). (bloodjournal.org)
  • Collectively, this is the first report to demonstrate the effectiveness of mollugin as a candidate for a chemotherapeutic agent in OSCCs via the upregulation of the HO-1 and Nrf2 pathways and the downregulation of NF- κ B. (hindawi.com)
  • In other cell types, the S100B/RAGE interaction triggers signaling pathways including NF-kB activation and translocation, MAP kinases, and p53. (ahajournals.org)
  • induce apoptosis
  • The absolute stereochemistry of the naturally occurring metabolite revealed it to be (+)-Cytotrienin A. Cytotrienin A has a median effective dose value of 7.7 nM to induce apoptosis on HL-60 cells. (wikipedia.org)
  • induction
  • It is thought that erythroid differentiation is primarily dependent on the presence and induction of erythroid transcriptional factors such as GATA-1, FOG-1 and EKLF, as well as the suppression of myeloid/lymphoid transcriptional factors such as PU.1. (wikipedia.org)
  • CFU-e progenitors enter the cell cycle at the time of GATA-1 induction and PU.1 suppression in a developmental manner rather than due to EpoR signaling. (wikipedia.org)
  • vitro
  • In conclusion, these in vitro results provide direct evidence that the interaction of RAGE and its ligand S100B induce myocyte apoptosis by activating p53 and inhibiting MAP kinase and NF-κB signaling. (ahajournals.org)
  • Its activity was defined in the mid 1960s by immunologists who sought to replicate, in vitro, key features of cell-mediated immunity ( 1 ). (rupress.org)
  • cytoplasmic
  • Similar to apoptosis, staining techniques can be used to identify paraptotic cells by highlighting the translocation of phosphatidylserine from the plasma membrane cytoplasmic (inner) leaflet to the cell surface or outer leaflet. (wikipedia.org)
  • 1997
  • Simon H-U, Yousefi S, Schranz C, Schapowal A, Bachert C, Blaser K (1997) Direct demonstration of delayed eosinophil apoptosis as a mechanism causing tissue eosinophilia. (springer.com)
  • endothelial cells
  • We found that endothelin-1 (ET-1) is an autocrine/paracrine survival factor I from serum deprivation-induced apoptosis for cultured rat endothelial cells. (nii.ac.jp)
  • Endothelial cells also underwent apoptosis by the addition of nitric oxide donors and natriuretic peptides, whose effects were also antagonized by ET-1. (nii.ac.jp)
  • These observations suggest that apoptosis survival activity of ET-1 in endothelial cells is not restricted to serum deprivation-induced apoptosis. (nii.ac.jp)
  • Publications] Shichiri M.et al: 'Endothelin-1 as an autocrine/paracrine apoptosis survival tactor for endothelial cells' Hypertension. (nii.ac.jp)
  • mechanism
  • Publications] Suenobu N.et al: 'Natriuretic peptides and nitric oxide induee endothelial apoptosis via cyclic GMP-dependent mechanism' Arteriosclerosis Thrombosis & Vascular Biology. (nii.ac.jp)
  • We thus investigated the effects and putative mechanism of apoptosis induced by mollugin in human oral squamous cell carcinoma cells (OSCCs). (hindawi.com)
  • upregulation
  • In humans, upregulation of RASD1 leading to increased apoptosis has been observed in several human cancer cell lines such as DU-154 human prostate cancer cells and in human breast cancer cells MCF-7. (wikipedia.org)
  • Proteins
  • Once ruptured, particles and substances are released, including: (1) high mobility group B-1 (HMGB1) (2) heat shock proteins and (3) various other proteases. (wikipedia.org)
  • While the presence of inactive MST/Krs proteins and dominant negative c-Jun completely inhibited apoptosis. (wikipedia.org)
  • regulation
  • The journal publishes original observations on genetic, cellular, biochemical, structural and molecular aspects of DNA repair, mutagenesis, cell cycle regulation, apoptosis and other biological responses in cells exposed to genomic insult, as well as their relationship to human disease . (elsevier.com)
  • tumor
  • This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. (wikipedia.org)
  • Tumor necrosis factor alpha (TNF-alpha) is a signaling molecule that interacts with one of two receptors on cells targeted for apoptosis. (wikipedia.org)
  • inhibitors
  • The use of specific kinases inhibitors may provide excellent tools to prevent RPE apoptosis specifically in RPE diseases involving ROS and other stress-related compounds such as in AMD. (epfl.ch)
  • Therefore, paraptosis was concluded to differ from apoptosis (cell death type 1) in being unaffected by inhibitors of apoptosis. (wikipedia.org)
  • nuclear
  • Mollugin-induced cell death involved apoptosis, characterized by the appearance of nuclear shrinkage, flow cytometric analysis of sub-G 1 phase arrest, and annexin V-FITC and propidium iodide staining. (hindawi.com)
  • Furthermore, mollugin induced the activation of p38, ERK, and JNK and the expression of heme oxygenase-1 (HO-1) and nuclear factor E2-related factor 2 (Nrf2). (hindawi.com)
  • Paraptosis lacks several of the hallmark characteristics of apoptosis, such as membrane blebbing, chromatin condensation, and nuclear fragmentation. (wikipedia.org)
  • Paraptosis does not demonstrate nuclear fragmentation, formation of apoptotic bodies, or definitive demonstration of chromatin condensation - all seen in apoptosis. (wikipedia.org)