• Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. (aatbio.com)
  • The MTT assay is a colorimetric assay that can assess the viability of cells by quantitation of the reduction of the yellow substrate MTT (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) to a product that has a purple color. (nih.gov)
  • This assay can measure the cytotoxicity of a chemical or drug by determining the affect of treatment on cell viability. (nih.gov)
  • o Assays of cellular or molecular phenotypes, viability or apoptosis. (nih.gov)
  • The neuroprotective effects of conditioned medium (CM) of hESCs (H9), wt hiPSCs (WTC-11) and the ALS iPSCs, on MN apoptosis and viability, formation and maintenance of neurites, mitochondrial activity and expression of inflammatory genes, were examined. (springer.com)
  • Cell viability was examined with Cell Counting Kit‑8 assays. (spandidos-publications.com)
  • Changes in cell viability, apoptosis, lactate dehydrogenase release, and cell cycle analysis were measured by cell counting kit-8 assay, annexin V/ propidium iodide staining, and enzyme-linked immunosorbent assay, respectively. (medsci.org)
  • Cell viability was detected by MTT assay. (medscimonit.com)
  • The non-toxic nature of [6]-gingerol was confirmed by viability assays on rapidly dividing normal mouse colon cells. (amrita.edu)
  • The anticancer mechanistic studies reveal that RuRe-1 and RuRe-2 can induce apoptosis through the regulation of cell cycle, depolarization of mitochondrial membrane potential (MMP), elevation of intracellular reactive oxygen species (ROS), and caspase cascade. (frontiersin.org)
  • Treat cells with test compounds for a desired period of time to induce apoptosis, and create positive and negative controls. (aatbio.com)
  • Quartz dust is known to induce apoptosis in vitro and in vivo. (cdc.gov)
  • The regional and segmental analysis of the global apoptosis assays market is also provided in the research report. (knnit.com)
  • Pan-caspase inhibition decreased CD-associated Bax expression and apoptosis but did not restore CYP1A1 activity. (nih.gov)
  • Apoptosis is induced through the mitochondrial pathway resulting in caspase-9 activation and downstream caspase-3 activation. (cdc.gov)
  • Caspase 3/7 activation is widely accepted as a reliable indicator for cell apoptosis. (aatbio.com)
  • Caspase Apoptosis assays monitor apoptosis by measuring the degree of the caspace (family of cysteine proteases) activity, an indicator of apoptosis. (nih.gov)
  • Caspase apoptosis assays measure the cleavage of the DEVD-AFC (Amino Tri-fluoromethyl Coumarin substrate to free AFC. (nih.gov)
  • PU may inhibit the expression of caspase-3 and Bax, which are closely related to cell apoptosis, and promote the protein expression of Bcl-2 ( 11 , 12 ). (spandidos-publications.com)
  • Interestingly, caspase-9-deficient Jurkat cells or Bcl-2- and Bcl-x L -overexpressing cells were strongly resistant to hydralazine treatment, thereby demonstrating the dependence of hydralazine-induced apoptosis on the mitochondrial death pathway. (oncotarget.com)
  • RIP-1 or c-FLIPL siRNA markedly induced cell apoptosis and enhanced caspase-8 and caspase-3 activities. (medscimonit.com)
  • Downregulating RIP-1 and/or BAY11-7082 significantly reduced NF-kB transcriptional activity, blocked cells in G0/G1 phase, weakened proliferation, elevated caspase-8 and caspase-3 activities, and promoted cell apoptosis. (medscimonit.com)
  • Astrocytes exposed to 2, 3, and 4 h of OGD exhibited increased cell injury and apoptosis, and the protein levels of caspase-12, cleaved caspase-3, NLRP3 inflammasome components, and IL-1β were also significantly elevated. (medsci.org)
  • Interestingly, pretreatment with the caspase-12-specific inhibitor Z-ATAD-FMK attenuated cell injury and apoptosis and decreased the levels of NLRP3, caspase-1, IL-1β, and cleaved caspase-3 in the OGD group. (medsci.org)
  • Caspase-12 activation and processing have been detected following ischemic stroke, but the role of caspase-12 in apoptosis and inflammation is controversial. (medsci.org)
  • Experiments have shown that caspase-12 is responsible for further activation of caspase-3 and ERS-induced cell apoptosis in astrocytes following ischemic stroke [ 5 - 7 ]. (medsci.org)
  • The hemolysis assay can quantify the acute in vitro hemolytic properties of nanoparticles. (nih.gov)
  • Apoptosis was measured in rat alveolar macrophage NR8383 cells challenged in vitro with respirable quartz or kaolin dust and with the dusts pretreated with dipalmitoyl phosphatidylcholine (DPPC) to model conditioning of respired dusts by interaction with a primary phospholipid component of pulmonary surfactant. (cdc.gov)
  • For this study, quartz and kaolin were compared as dusts of similar cytotoxicity in some in vitro assays but of differing pathogenic potential: quartz can cause significant pulmonary fibrosis while kaolin generally does not. (cdc.gov)
  • Many of the in vitro biological and disease assays currently used to study the effects of specific compounds or genetic perturbations can be adapted to high throughput formats. (nih.gov)
  • In vitro assay also demonstrated that ADSC markedly attenuated CTX-induced senescence and apoptosis of granulosa cell. (biomedcentral.com)
  • Curcumin attenuates hydroxychloroquine-mediated apoptosis and oxidative stress via the inhibition of TRPM2 channel signalling pathways in a retinal pigment epithelium cell line. (ilexlife.com)
  • Apoptosis inhibition causes cells to snub internal or external stimuli that would normally initiate apoptosis. (vkay.net)
  • The info display neutralizing antibodies in sera after a month relaxing time taken between the final problem and vaccination, assessed with a hemagglutination inhibition assay. (nonamimaho.com)
  • Under IL-1 beta treatment with or without Dex, the inhibition of lncH19 lead to an increase cell proliferation, a decrease in cell apoptosis, an increase in the protein level of inflammatory-related genes, and the phosphorylation of P65, ICAM-1, VCAM-1, and inflammatory cytokines. (researchsquare.com)
  • Extracellular Gal-3 is secreted via a non-classical pathway (because of lack of signal peptides) and can bind to the cell surface through glycosylated proteins, thereby triggering or modulating cellular responses such as mediator release or apoptosis. (nature.com)
  • The assay also has the potential to aid in the selection of cancer patients likely to respond to drugs targeting the apoptosis pathway. (nih.gov)
  • Apoptosis is most often mediated by extrinsic pathway via FAS or CD95 or apoptosis antigen 1 (APO-1) or tumor necrosis factor receptor superfamily 6 (TNFRSF6), a cell surface death receptor. (medscape.com)
  • ALPS is rarely caused by a defect of mitochondrial apoptosis or intrinsic pathway. (medscape.com)
  • A heterozygous germline gain of function mutation encoding NRAS, an oncogene, leading to a failure of apoptosis in response to interleukin-2 withdrawal was identified as the first intrinsic pathway defect causing ALPS. (medscape.com)
  • The extrinsic pathway of apoptosis. (medscape.com)
  • While assays for plate readers are optimized to isolate a particular pathway, high-content screening enables visualization of specific, toxic, and off-target effects in the same screen or workflow. (genengnews.com)
  • GM‑induced ototoxicity may be closely related to the upregulation of p53 expression and the activation of endogenous mitochondrial apoptosis pathways, and PU could protect cochlear hair cells from GM‑mediated damage by reducing the production of ROS and inhibiting the mitochondria‑dependent apoptosis pathway. (spandidos-publications.com)
  • Moreover, we demonstrate that hydralazine triggered the mitochondrial pathway of apoptosis by inducing Bak activation and loss of the mitochondrial membrane potential. (oncotarget.com)
  • The authors conclude that activation of the ERK pathway plays a role in asbestos induced apoptosis. (cdc.gov)
  • The following sections provide examples of Assay Files for common types of assays identified across nanotechnology resources. (nih.gov)
  • The types of assays sought and instructions for applying to the program follow. (nih.gov)
  • Traditional assays performed in needle biopsies only measure individual Bcl-2 family proteins and do not capture the protein-protein interactions. (nih.gov)
  • Protein concentration was determined with Bio-Rad protein assay. (microbiologystudents.com)
  • this results in cytoplasmic recruitment of a protein known as the FAS-associated death domain (FADD), followed by recruitment of procaspase 8 and procaspase 10 and resultant cellular apoptosis. (medscape.com)
  • Since p53 was identified as a tumor suppressor protein in late 1980's, various studies have focused on its contributions on cell cycle arrest, senescence, apoptosis and autophagy [ 8 , 9 ]. (ijpsonline.com)
  • Role of extracellular signal-regulated protein kinases in apoptosis by asbestos and H2O2. (cdc.gov)
  • The role of extracellular signal regulated protein kinases in cellular apoptosis induced by asbestos (1332214) and hydrogen- peroxide (7722841) was examined. (cdc.gov)
  • The extent of activation of extracellular signal regulated protein-kinase-2 (ERK2) and c-jun-NH2- terminal-protein-kinase-1 (JNK1) was determined by immunoprecipitation assays. (cdc.gov)
  • We investigated the hypothesis that apoptosis plays a critical role in lung injury and down-regulation of CYP1A1 induction in mixed exposures to CD and PAHs. (nih.gov)
  • Within this assay, viruses were used at 100 g/mL. pone.0128785.s001.tif (646K) GUID:?24132513-5C95-456B-A430-9B52AAC091D5 S2 Fig: Induction of neutralizing antibodies with the pressurized H3N8 vaccine. (nonamimaho.com)
  • mouse brain resulted in an induction of apoptosis similar to Ultrathin sections were stained with uranyl acetate and lead what is observed in natural infection. (cdc.gov)
  • Apoptosis induction vested and lysed at 48 h postinjection. (cdc.gov)
  • Each cell line should be evaluated on an individual basis to determine the optimal cell density for apoptosis induction. (aatbio.com)
  • Effects of phospholipid surfactant on apoptosis induction by respirable quartz and kaolin in NR8383 rat pulmonary macrophages. (cdc.gov)
  • Induction of apoptosis was assessed by the 4',6-diamidino-2- phenylindole staining assay. (cdc.gov)
  • Flow cytometric analysis revealed that compounds 3d and 3h arrested the HL-60â ¯ cells in sub G1 phase by induction of apoptosis . (bvsalud.org)
  • Apoptosis Assay Laboratories manufactures the tnfalpha u937 apoptosis assay reagents distributed by Genprice. (microbiologystudents.com)
  • NR8383 cells exposed to native quartz at concentrations from 50 to 400 microg/ml for 6 h showed a dose-dependent increase in apoptosis measured by the TdT-mediated dUTP-fluorescein nick end labeling (TUNEL), cell death ELISA, and DNA ladder formation assays, while native kaolin induced significant response only at the higher concentrations and only in the TUNEL and ELISA assays. (cdc.gov)
  • Crocidolite caused a three fold increase in apoptosis over the control value. (cdc.gov)
  • Cytotoxicity assay shows that RuRe-1 and RuRe-2 exhibit higher anticancer activity than cisplatin, and exist certain selectivity toward human cancer cells over normal cells. (frontiersin.org)
  • Enhanced cytotoxicity and decreased mutagenicity is concomitant with a dramatic increase in the number of cells undergoing apoptosis when BG is combined with PM, melphalan, or chlorambucil at 72-94 h after treatment. (nih.gov)
  • The Hepatocarcinoma Cytotoxicity Assay tests the cytotoxicity of nanoparticle formulations in human hepatocarcinoma cells (Hep G2). (nih.gov)
  • Shorter durations were used to study apoptosis and longer durations for multiparameter cytotoxicity studies. (genengnews.com)
  • CCK‑8 and apoptosis assays were performed to investigate cytotoxicity of celastrol and effect on apoptosis on orbital fibroblasts. (spandidos-publications.com)
  • The 2023 Global Forecast for Apoptosis Assays (2024-2029 Outlook)-High Tech & Emerging Markets Report, published by Barnes Reports, contains timely and accurate market statistics and forecasts on the market for over 50 countries. (barnesreports.com)
  • It has been proved that multiple types of cancer including breast cancer are developed due to malfunctioning of the genes, which code antiapoptotic proteins, apoptosis inhibitors or tumor suppressors [ 6 , 7 ]. (ijpsonline.com)
  • Synthesis of carbazole derivatives containing chalcone analogs as non-intercalative topoisomerase II catalytic inhibitors and apoptosis inducers. (bvsalud.org)
  • In addition, TME5C counteracted calcineurin inhibitor-induced apoptosis and vascular permeability in HUVECs and HHSECs. (haematologica.org)
  • Bcl-2 proteins are negative regulators of apoptosis that control cell survival and death. (nih.gov)
  • Researchers at the NCI developed a multiplex assay to determine the efficacy of apoptosis-related drugs targeting the Bcl2 family of proteins or aid in the selection of cancer patients likely to respond. (nih.gov)
  • Appropriate assays include: o Biochemical or cell-based assays of activity, behavior or interaction of proteins and other molecules of interest. (nih.gov)
  • o Assays involving mutant proteins linked to neurodegeneration or neuroprotection. (nih.gov)
  • Apoptosis and cellular senescence-related proteins were measured by Western blotting. (medsci.org)
  • These changes were markedly reversed by EPA administration, and the expressions of apoptosis and cellular senescence-related proteins were reversed by EPA treatment. (medsci.org)
  • Cultured HUVECs under oxidative and glucolipotoxic stresses revealed accelerated senescence and increased apoptosis. (medsci.org)
  • DVTP nick-end labeling (TUNEL) assay with the In Situ Cell determined according to the manufacturer's protocol. (cdc.gov)
  • Hydroxychloroquine Attenuates Acute Inflammation (LPS)-Induced Apoptosis via Inhibiting TRPV1 Channel/ROS Signaling Pathways in Human Monocytes. (ilexlife.com)
  • The proinflammatory factor interleukin-1β (IL-1β) was demonstrated to cause inflammation as well as cell apoptosis during the process of BRB breakdown. (molvis.org)
  • Capsid gene delivery into the striatum of mouse brain or interskeletal muscle resulted in cell death and inflammation, likely through capsid-induced apoptosis in vivo. (cdc.gov)
  • Semithin (1.0-µm) sections resulted in apoptosis and inflammation of muscle cells. (cdc.gov)
  • Western blot assay and RT-qPCR were utilized to determine the expression of Bax, Bcl-2, TGF- β 1, and smad3. (hindawi.com)
  • M2 macrophage polarization-associated genes were measured by using qRT-PCR and Western blot assays. (hindawi.com)
  • Apoptosis assays market which was USD 3315.7 million in 2021, is expected to reach USD 6558.41 million by 2029, at a CAGR of 8.9% during the forecast period 2022 to 2029. (vkay.net)
  • Annexin A5 is used as a non-quantitative probe to detect cells that have expressed phosphatidylserine (PS) on the cell surface, an event found in apoptosis as well as other forms of cell death. (wikipedia.org)
  • The annexin A5 affinity assay typically uses a conjugate of annexin V and a fluorescent or enzymatic label, biotin or other tags, or a radioelement, in a suitable buffer (annexin V binding to aminophospholipids is Ca2+ dependent). (wikipedia.org)
  • The assay combines annexin V staining of PS and PE membrane events with the staining of DNA in the cell nucleus with propidium iodide (PI) or 7-Aminoactinomycin D (AAD-7), distinguishing viable cells from apoptotic cells and necrotic cells. (wikipedia.org)
  • It was further confirmed by Annexin -V- FITC binding assay. (bvsalud.org)
  • In spite of, or perhaps in part due to the requirement of ATP for apoptosis, the intracellular ATP in apoptotic cells is ultimately depleted. (elifesciences.org)
  • The examples shown here are from the development of an HCT116 spheroid assay for evaluating spheroid morphological changes in addition to the incidence of apoptotic cells in the well. (genengnews.com)
  • A failure of apoptosis leads to inappropriate cell survival and diseases associated with excessive accumulations of cells such as cancer, chronic inflammatory conditions, and autoimmune diseases. (medscape.com)
  • Matrigel plug assays showed that TME5C stimulates in vivo angiogenesis. (haematologica.org)
  • In vivo studies revealed that ADSCs transplantation reduced the apoptosis of ovarian granulosa cells and secretion of follicle-stimulating hormone. (biomedcentral.com)
  • Using a quantitative adhesion assay, we estimated that 120 min after plating only 30% of integrin a11-deficent cells were able to attach to collagen I. In contrast, at the same time point, 70% of integrin a2-knockdown hMSC were attached while integrin a1- and shRNA control hMSC have already reached 100% cell adhesion. (uni-muenchen.de)
  • Although there are a variety of stimuli that can provoke apoptosis, these stimuli converge into the activation of a proteolytic cascade of cysteine proteases, called caspases. (elifesciences.org)
  • The Tnfalpha U937 Apoptosis Assay reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (microbiologystudents.com)
  • Apoptosis assays cell-based apoptosis detection assays apoptosis, also known as programmed cell death, is a growth-limiting regulatory mechanism in which cells can kill themselves in response to extracellular signals due to irreversible cellular or DNA damage. (vkay.net)
  • Leukocyte endothelial adhesion was assessed through a cell-based assay using lymphoblastoid cells. (molvis.org)
  • Colorimetric adhesion assay was used for estimation of the extent of cells attachment. (uni-muenchen.de)
  • Paradichlorobenzene: Estimation of its mutagenic potential in the Salmonella typhimurium plate incorporation mutagenicity assay. (cdc.gov)
  • Cancer-associated variants and a common polymorphism of MUTYH exhibit reduced repair of oxidative DNA damage using a GFP-based assay in mammalian cells. (nih.gov)
  • However, direct evaluation of OG:A mismatch repair efficiency in mammalian cells has not been assessed due to the lack of an appropriate assay. (nih.gov)
  • In addition, it is desirable to demonstrate the selectivity and reproducibility of response of the assay to a small but diverse collection of a few hundred compounds, such a collection of FDA approved drugs or other bioactive molecules. (nih.gov)
  • The global market for apoptosis assays demonstrate fragmented structure due to presence of large and small regional and international players. (knnit.com)
  • PROPOSAL CONTENT Proposals should demonstrate reproducibility of the proposed assay in a low-to-moderate throughput setting (e.g., 24-well format) and should be feasible for adaptation to an automated, high-throughput screening approach. (nih.gov)
  • Apoptosis of alveolar epithelial cells during acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) is a critical pathological event that seriously endangers the health of patients. (nih.gov)
  • In early apoptosis, cells lose membrane phospholipid asymmetry, whilst the integrity of the cell membrane is maintained. (beckman.com)
  • Phosphatidylserine (PS), a negatively charged phospholipid located in the inner surface of the plasma membrane, appears very early in apoptosis at the external cell surface. (beckman.com)
  • PU ameliorated the morphological changes of mouse cochlear hair cells and reduced the apoptosis rate of HEI‑OC1 cells after GM‑mediated damage. (spandidos-publications.com)
  • These features can be met by a broad range of assay types, including enzymatic and biochemical assays, and assays in intact cells or simple model organisms such as yeast or C. elegans. (nih.gov)
  • As a result, many anti-cancer strategies in drug development focus on restoring apoptosis in order to cause cell death in these specific cell populations and shrink tumours. (vkay.net)
  • Apoptosis is a form of programmed cell death, with important roles in development, tissue homeostasis, and immunity. (elifesciences.org)
  • Apoptosis is, thus, considered to be an energy-demanding process, requiring intracellular ATP for the execution of the cell death program. (elifesciences.org)
  • Apoptosis is a genetically regulated form of nonimmunogenic cell death. (medscape.com)
  • Apoptosis is programmed cell death characterized by the activation of catabolic enzymes, which is responsible for degradation of cellular components, shrinkage of the cell, condensation of chromatin and fragmentation of DNA [ 4 , 5 ]. (ijpsonline.com)
  • The concepts underlying cell death assays are relatively simple: if the drug kills tumor cells from an individual patient in a test tube, then the drug is more likely to be effective when administered to a patient. (cancer.org)
  • At the end of the culture period, a measurement is made of cell injury, which correlates directly with cell death (apoptosis). (cancer.org)
  • The general measurements of cell death are the DISC assay method, the MTT assay method, the ATP assay method, and the flurescein diacetate assay method. (cancer.org)
  • The effects of POF modeling and transplantation were determined by estrous cycle analysis, histopathological analysis, immunohistochemical staining and apoptosis-related marker. (biomedcentral.com)
  • Enhanced apoptosis in the leukocytes of peripheral blood of PEM patients may be a marker of increased infection and immune disturbances. (who.int)
  • The LDH assay is a measure of membrane integrity. (nih.gov)
  • Description: TUNEL Apoptosis Detection Kit is designed to conveniently detecting DNA fragmentation. (microbiologystudents.com)
  • They were conjugated with a monoclonal antibody (Ab) to p53 and cmHp70.1 to Hsp70 for detection of cancer cell apoptosis that was demonstrated in the experiment by fluorescent confocal microscopy both for breast carcinoma cells and for thyroid tissue. (intechopen.com)
  • Death Assay Kit (Roche Diagnostic Corp., Indianapolis, IN) transfected with pcWNV-Cp-DJY or pcDNA3.1 were har- and visualized by fluorescent microscopy. (cdc.gov)
  • NAC and PD-98059 both significantly reduced the level of crocidolite induced apoptosis. (cdc.gov)
  • The results from Topo II mediated DNA relaxation assay showed that CDCAs could significantly inhibit the activity of Topo II, and the structure-activity relationship indicated the halogen substituent in phenyl ring play an important role in the activity. (bvsalud.org)