Oligonucleotide-directed mutagenesisMechanisms of mutagenesisChemical mutagenesisSaturation mutagenesisMutationExperimentsCisgenesisSomatic hypermutationAltersCloningRadiationsNucleasesPlantExperimentalMutantHeritableChemicalsMainSequenceReceptorLaboratoryPublicationEnzymeConstructsAssemblyTechniqueSiteMethodCancerIntroductionSeriesPlaceOrderScienceToolMakeShown
Oligonucleotide-directed mutagenesis1
- The working group prepared a list of seven new plant breeding techniques: zinc finger nuclease (ZFN) technology, oligonucleotide-directed mutagenesis (ODM), cisgenesis and intragenesis, grafting on GM-rootstock, RNA-dependent DNAmethylation, agro-infiltration "sensu stricto," and reverse breeding. (frontiersin.org)
Mechanisms of mutagenesis2
- Kinetic models reveal the in vivo mechanisms of mutagenesis in microbe" by Barbara E. Wright, Karen H. Schmidt et al. (umt.edu)
- Small Indels in the Androgen Receptor Gene: Phenotype Implications and Mechanisms of Mutagenesis. (medscape.com)
Chemical mutagenesis1
- Due to its long safety record, organisms obtained by physical and chemical mutagenesis are exempt from the provisions of the GMO legislation in the EU. (frontiersin.org)
Saturation mutagenesis3
- Saturation mutagenesis is a powerful technique for engineering proteins, metabolic pathways and genomes. (nature.com)
- In spite of its numerous applications, creating high-quality saturation mutagenesis libraries remains a challenge, as various experimental parameters influence in a complex manner the resulting diversity. (nature.com)
- Following the seminal work of Michael Smith concerning site-directed mutagenesis 1 , saturation mutagenesis (SM) has emerged as an indispensable technique in molecular biology for introducing targeted sequence variations at virtually any DNA region. (nature.com)
Mutation5
- Mutagenesis (/mjuːtəˈdʒɛnɪsɪs/) is a process by which the genetic information of an organism is changed by the production of a mutation. (wikipedia.org)
- Here we describe the use of New England Biolabs' Gibson Assembly Master Mix in two different mutagenesis experiments: first, multiple mutations of the lacZ gene ( Figure 1 ) and second, the mutation of 5 of the 6 nucleotides at position 174-179 of eGFP from CTGACC to TTCTAT in order to change the amino acid sequence from LeuThr to PheTyr ( Figure 2 ). (genengnews.com)
- And two, the mutagenesis generator proves after a long mutation. (jove.com)
- The second generation of our QuikChange method that provides improved fidelity over our original kit, while maintaining greater than 80% mutation efficiency for single site mutagenesis. (selectscience.net)
- Some act by increasing the viral mutation rate causing "death by mutagenesis" of the virus. (researchsquare.com)
Experiments1
- Our model predicted several interactions ( i.e ., hydrophobic interactions, hydrogen bonds, and amide-pi stacking) between ALK peptides and ALKR, and several of our substitution and mutagenesis experiments were consistent with the predicted model. (unl.edu)
Cisgenesis5
- The European Food Safety Authority (EFSA) has published the criteria for risk assessment of plants produced by targeted mutagenesis, cisgenesis, and intragenesis. (isaaa.org)
- On April 28, 2022, the European Commission requested EFSA, in accordance with Article 31 of Regulation (EC) No 178/2002, to develop a statement on possible criteria for risk assessment of plants produced by targeted mutagenesis, cisgenesis, and intragenesis. (isaaa.org)
- The statement was requested as advice for consideration by the European Commission to support the ongoing policy initiative on plants produced by targeted mutagenesis and cisgenesis. (isaaa.org)
- To develop the statement, EFSA considered previously published EFSA Opinions on targeted mutagenesis, cisgenesis, and intragenesis and those assessment aspects discussed by the GMO Panel in the EFSA Opinions on plants developed through synthetic biology which are relevant for the risk assessment of plants developed through targeted mutagenesis, cisgenesis, and intragenesis. (isaaa.org)
- The history of use includes the history of safe use (HoSU) for consumers and/or animals and familiarity with the environment which are key elements in the assessment of plants produced by targeted mutagenesis, cisgenesis, and intragenesis. (isaaa.org)
Somatic hypermutation3
- In somatic hypermutation, targeted and rampant mutagenesis alters the sequences of the expressed heavy and light chain variable (VDJ) regions. (biomedcentral.com)
- Transcription-coupled mutagenesis initiates class-switch recombination and somatic hypermutation. (biomedcentral.com)
- Kinetic models which have demonstrated predictive value are described and are shown to simulate mutagenesis in vivo in Escherichia coli, the p53 tumor suppressor gene, and somatic hypermutation. (umt.edu)
Alters1
- Targeting key residues of the PT-pathway by site directed mutagenesis significantly alters the pH-activity profile of these variants and in presence of H 2 their cofactor is trapped in an intermediate state indicative of precluded proton-transfer. (nature.com)
Cloning1
- GenScript's site-directed mutagenesis services provide a fast, cost-saving, way to get 100% accurate mutant DNA clones, without having to spend your time and money on mutagenesis kits to do PCR mutagenesis, cloning, and sequencing in your own lab. (genscript.com)
Radiations1
- Similarly, laccase production has also been improved through UV radiations based mutagenesis, and UV mutant i.e. (scite.ai)
Nucleases2
- Efficient targeted mutagenesis in the monarch butterfly using zinc-finger nucleases. (umassmed.edu)
- Title : High frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells Personal Author(s) : Fu, Yanfang;Foden, Jennifer A.;Khayter, Cyd;Maeder, Morgan L.;Reyon, Deepak;Joung, J. Keith;Sander, Jeffry D. (cdc.gov)
Plant3
- Plant mutagenesis is rapidly coming of age in the aftermath of recent developments in high-resolution molecular and biochemical techniques. (hindawi.com)
- New Breeding Techniques (NBTs) include several new technologies for introduction of new variation into crop plants for plant breeding, in particular the methods that aim to make targeted mutagenesis at specific sites in the plant genome (NBT mutagenesis). (frontiersin.org)
- The decision was based in a context of conventional plant breeding using mutagenesis of crop plants by physical or chemical treatments. (frontiersin.org)
Experimental1
- Improvement of seed yield in vicia faba by using experimental mutagenesis ii. (eurekamag.com)
Mutant3
- In a laboratory setting, mutagenesis is a useful technique for generating mutations that allows the functions of genes and gene products to be examined in detail, producing proteins with improved characteristics or novel functions, as well as mutant strains with useful properties. (wikipedia.org)
- The fastest, and usually the most cost-effective, way to get the mutant DNA constructs you need is to order Express Mutagenesis bundled with gene synthesis using our online quotation form . (genscript.com)
- A mutant library was created with insertional mutagenesis via electroporation in order to transform a BASTA resistance gene into conidial nuclei. (uni-muenchen.de)
Heritable1
- In nature mutagenesis can lead to cancer and various heritable diseases, and it is also a driving force of evolution. (wikipedia.org)
Chemicals2
- This mutagenesis method is simple, fast, free of toxic chemicals, and well-suited for forward genetic screening and transgene integration. (jove.com)
- The two main advantages of this technique are one, the mutagenesis protocol is very simple and do not require the toxic chemicals. (jove.com)
Main1
- The Asian Mouse Mutagenesis Useful resource Affiliation (AMMRA) is a non-profit group consisting of main useful resource and analysis establishments with rodent experience from inside the Asia Pacific area. (eumorphia.org)
Sequence1
- We achieved high quality mutagenesis results and were able to complete the substitutions in our sequence. (selectscience.net)
Receptor1
- To define the histamine-binding site of this receptor, molecular modeling and site-directed mutagenesis were used to predict and alter amino acids residing in the histamine-binding pocket. (aspetjournals.org)
Laboratory1
- Format: Text / Subject: Mutagenesis and Gene Expression Regulation / Genre: Articles / Publisher: Cold Spring Harbor Laboratory. (nih.gov)
Publication1
- Scholars@Duke publication: Ribonucleotides and Transcription-Associated Mutagenesis in Yeast. (duke.edu)
Enzyme3
- We also offer solutions for automation, site-directed mutagenesis, as well as your favorite restriction enzyme, ligase or competent cell products. (neb.com)
- Site-directed mutagenesis facilitates life science research by creating specific DNA mutations (including point mutations, deletion mutations, and insertion mutations) to enable studies of gene regulatory elements, DNA-protein interactions, protein structure/function, enzyme active sites, and novel proteins. (genscript.com)
- Study of the regulatory properties of glucokinase by site-directed mutagenesis: conversion of glucokinase to an enzyme with high affinity for glucose. (diabetesjournals.org)
Constructs1
- Complete kit provides all reagents necessary to support mutagenesis (at single sites), for large constructs, and for use with electroporation competent cells. (selectscience.net)
Assembly1
- Site-directed mutagenesis of lacZ using Gibson Assembly. (genengnews.com)
Technique1
- Site-directed mutagenesis (SDM) is a commonly used technique for introducing mutations into a gene of interest. (genengnews.com)
Site5
- Learn how to create substitutions, deletions or insertions in 3 easy steps with the Q5 Site-Directed Mutagenesis Kit. (neb.com)
- The Q5 Site-Directed Mutagenesis Kit allows rapid site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours. (neb.com)
- Using our sophisticated gene synthesis and mutagenesis techniques, GenScript can introduce mutations at any site. (genscript.com)
- QuikChange II Site-Directed Mutagenesis. (selectscience.net)
- Structural and biochemical characterization of two heme binding sites on α1-microglobulin using site directed mutagenesis and molecular simulation. (lu.se)
Method2
- Conclusions AlbaTraDIS provides a simple and rapid method to analyse multiple transposon mutagenesis data sets allowing this technology to be used at large scale. (biorxiv.org)
- Aiyar, A & Leis, J 1993, ' Modification of the megaprimer method of PCR mutagenesis: Improved amplification of the final product ', BioTechniques , vol. 14, no. 3, pp. 366+368-369. (northwestern.edu)
Cancer2
- RÉSUMÉ Des études en milieu hospitalier ont révélé des fréquences relatives très élevées du cancer oropharyngé au Yémen. (who.int)
- La présente étude a estimé les fréquences relatives du cancer de la cavité buccale et du pharynx chez des patients yéménites atteints de cancers enregistrés en 2007 et 2008. (who.int)
Introduction1
- Mutagenesis research has two aims: (1) to understand the molecular mechanisms leading to mutations, and (2) to prevent a thoughtless introduction of mutagenic agents into our environment. (powells.com)
Series1
- Both aspects, namely, basic and applied, will be treated in the new series Advances in Mutagenesis Research. (powells.com)
Place2
- For the mutagenesis, prepare a filter paper to fit a 60 millimeter plate with an approximately 25 millimeter square hole at the center, and place it onto an unseeded plate. (jove.com)
- instead, a convergence has taken place between conventional mutagenesis and NBTs, in particular due to the possibilities of TILLING methods that allow the fast detection of mutations in any gene of a genome. (frontiersin.org)
Order1
- For mutagenesis from a template previously synthesized by GenScript (Cat No. SC1626), please Quote with your previous order number to request a quote for price and turnaround time. (genscript.com)
Science1
- Mutagenesis as a science was developed based on work done by Hermann Muller, Charlotte Auerbach and J. M. Robson in the first half of the 20th century. (wikipedia.org)
Tool1
- Transposon mutagenesis has been proven to be a powerful tool to identify genes and networks which are involved in survival and fitness under a given condition by simultaneously assaying the fitness of millions of mutants, thereby relating genotype to phenotype and contributing to an understanding of bacterial cell biology. (biorxiv.org)
Make1
- Mutagenesis in wheat: An approach to make saline green! (gu.se)
Shown1
- Prices and turnaround times shown for Cat No. SC1441 apply to mutagenesis ordered at the same time as new gene synthesis orders. (genscript.com)