1 In HI assays, viruses were tested for their ability to agglutinate red blood cells in the presence of ferret antisera previously raised against reference viruses. (health.gov.au)
Haemagglutination inhibition (HI) assays performed on dates between 1990 and 2021 by the WHO Collaborating Centre for Reference and Research on Influenza, London, UK on viruses collected between 1961 and 2021. (gla.ac.uk)
We assessed the antigenic distance between the HA of representative swine H1 and human seasonal vaccine strains (1978 to 2015) in hemagglutination inhibition (HI) assays using a panel of monovalent antisera raised in pigs. (bvsalud.org)
Immunoblot assays with polyclonal antiserum indicated that the 200 kDa protein was associated exclusively with haemagglutinating isolates and antibodies to this protein did not recognise epitopes on non-haemagglutinating isolates. (elsevierpure.com)
AntigenMap is an online resource developed specifically for antigenic cartography construction using the data from immunological experiments, such as hemagglutination inhibition, microneutralization, and Enzyme-linked immunosorbent (ELISA) assays. (hekademeia.org)
Hemagglutination inhibition (HI), microneutralization (MN), and Enzyme-linked immunosorbent (ELISA) assays are three commonly used techniques for antigenic variation characterization. (hekademeia.org)
Antibody5
Immunofluorescent staining of fluids with specific conjugated antisera, and the Haemagglutination-inhibition test and ELISA to check for antibody in blood are also helpful. (poultryworld.net)
The basic principle of blood typing involves observation of a simple antigen-antibody reaction leading to hemagglutination or clumping of RBCs. (sigmaaldrich.com)
Serological analyses demonstrated that homologous hemagglutination inhibition and microneutralization antibody titers were detectable in the ferrets after the first immunization with the AS03-adjuvanted vaccines that were further boosted by the second immunization. (nature.com)
A substance that inhibits or prevents hemagglutination: Judy found out that an antihemagglutinin could be an antibody that would suppress the coagulation of red blood cells. (wordinfo.info)
The E protein is presumed to carry recognition sites for the attachment of virus to receptors on susceptible cells and is responsible both for the haemagglutinating property exhibited by most flaviviruses and for stimulating the production of antibody demonstrated in haemagglutination-inhibition tests and protective immune responses in the host. (anipedia.org)
Inhibition test3
P6-740 was readily identified as a strain of Zika virus by the use of antiserum from monkeys in the standard hemagglutination-inhibition test and the plaque-reduction neutralization test in Vero cell cultures. (atmire.com)
several strain sa11-specific antibodies were found to have neutralizing and hemagglutination-inhibiting capacity. (liverpool.ac.uk)
In the past they could only be separated with difficulty in tests involving polyclonal antisera, 16, 17 but can now be differentiated by use of monoclonal antibodies. (anipedia.org)
Titers1
One computer virus mutant that varied at only two positions relative to the wild type had a >4-fold reduction in HI titers compared to homologous antisera. (tam-receptor.com)
Dataset includes HI titres measured between 16,213 viruses and antisera raised against 381 reference viruses. (gla.ac.uk)
Viruses from Ireland, the UK and the USA were antigenically characterised by the haemagglutination inhibition (HI) assay using post-infection ferret antisera and chicken red blood cells. (woah.org)
We tested the antigenic effect of these introduced substitutions by using hemagglutination inhibition (HI) data with monovalent swine antisera and antigenic cartography to evaluate the antigenic phenotype of the mutant viruses. (tam-receptor.com)
Prototype strains of viruses type A and B differed during the 10-year and antisera were provided by the WHO period (Figure 2). (who.int)
Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen. (lookformedical.com)
Briefly, a low rank matrix completion algorithm is applied to fill in the entries of the HI matrix by assuming each antigen i can be embedded into the r dimensional space as ui and antiserum j can be embedded into the r dimensional space as vj. (hekademeia.org)
Cross-reactivity1
This antiSerum has not been tested for cross-reactivity with other species. (nordicmubio.com)
Inhibits1
By using a standard amount of virus, a standard amount of blood cells, and serially diluting the antiserum, one can identify the concentration of the antiserum (the greatest dilution which inhibits hemagglutination). (wikipedia.org)
Influenza2
The antigenic properties of influenza viral isolates were analysed using the haemagglutination inhibition (HI) assay as previously described. (health.gov.au)
Human seasonal Influenza A(H3N2) haemagglutination inhibition data 1990-2021 from the WHO Collaborating Centre for Reference and Research on Influenza, London, UK. (gla.ac.uk)
Reagent1
A positive result occurs if there is clumping (hemagglutination) or rupture (hemolysis) of red blood cells exposed to the antiserum or typing reagent. (vin.com)
ELISA1
Bunnie antiserum spent my youth up against the purified LGBP and accustomed to create a meal ELISA method for quantitation of the necessary protein inside hepatopancreas and serum types of Michael. (cellcycleinhibitors.com)
Protein4
The antiSerum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. (nordicmubio.com)
A distinct elliptical precipitin arc results for each protein detectable by the antisera. (lookformedical.com)
western transfer and immunoenzymatic staining with affinity-purified monospecific antiserum was used to detect a 38-kd protein that bound to native and denatured dna cellulose. (liverpool.ac.uk)
Here, we describe DCFHP, a ferritin-based, protein-nanoparticle vaccine candidate that, when formulated with aluminum hydroxide as the sole adjuvant (DCFHP-alum), elicits potent and durable neutralizing antisera in non-human primates against known VOCs, including Omicron BQ.1, as well as against SARS-CoV-1. (stanford.edu)
Agglutination2
Hemagglutination, or haemagglutination, is a specific form of agglutination that involves red blood cells (RBCs). (wikipedia.org)
The reactivity of the antiserum is restricted to an allotype specific determinant on IgA2(m)2 molecule as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (nordicmubio.com)
Phenotype1
In the ABO system, the phenotype of an individual is usually determined by the hemagglutination reactions of the individual's RBCs with Anti-A, Anti-B, and Anti-AB antisera (forward grouping). (sigmaaldrich.com)
A ferritin-based COVID-19 nanoparticle vaccine that elicits robust, durable, broad-spectrum neutralizing antisera in non-human primates. (stanford.edu)
Virus5
It has two common uses in the laboratory: blood typing and the quantification of virus dilutions in a haemagglutination assay. (wikipedia.org)
One of them was capable of neutralizing virus infectivity and inhibiting haemagglutination, while the other had no significant neutralizing or haemagglutination-inhibiting capability, but did inhibit virus-mediated haemolysis. (microbiologyresearch.org)
Enhancement of dengue virus infection in monocytes by flavivirus antisera. (microbiologyresearch.org)
Isolation and identification of haemagglutination virus in embryonated duck eggs is also important. (poultryworld.net)
RESUME Afin de mieux comprendre la distribution annuelle du virus de la grippe dans notre pays, nous avons procédé à l'isolement et au typage de 45 virus provenant de 1043 patients atteints de maladies respiratoires aiguës au cours d'une étude sur 10 ans réalisée par le Centre national de la grippe en Iran. (who.int)
Specific2
Both species-specific antisera and chemical reagents, directed against specific red blood cell antigens, are used in in-vitro blood-typing methods. (vin.com)
Tests that are dependent on the clumping of cells, microorganisms, or particles when mixed with specific antiserum. (lookformedical.com)
Blood cells1
3. In physiology, to cause (red blood cells or bacteria) to clump together: Natasha wanted to know if she was Rh positive, so the doctor told her that it would be so if the antiserum agglutinated in her red blood cells. (wordinfo.info)
Human1
WHO-06-140 Pertussis Antiserum(human)1st IS-WHO international Standard. (kudiancn.com)
Genetic and antigenic analysis by hemagglutination inhibition and direct nucleotide sequencing of the HA1 region of the hemagglutinin gene were performed on two isolates, A/Peru/1641/99 and A/Peru/1798/99. (medscape.com)
Reference equine antisera to all 47 serotypes of human adenoviruses presently described have been prepared and evaluated by reciprocal neutralization and hemagglutination-inhibition tests. (nih.gov)
Hemagglutination inhibition (HI) titers are a major correlate of protection for influenza-related illness. (jci.org)
This illustration depicts what takes place during a hemagglutination inhibition test, in this case, using a virus, and a specific antiserum containing antibodies, and how these react with red blood cells (RBCs), when used in concert (top), and when used separately (bottom). (cdc.gov)
CDC characterizes influenza viruses through one or more tests including genomic sequencing , hemagglutination inhibition (HI) and/or neutralization assays. (cdc.gov)
The mAbs were characterized in ELISA, hemagglutination inhibition, western and neutralization assays. (nih.gov)
The influenza type A previously reported has been identified at Tulane University by hemagglutination-inhibition tests using 1950 A-prime antisera. (co.ke)
Human seasonal Influenza A(H3N2) haemagglutination inhibition data 1990-2021 from the WHO Collaborating Centre for Reference and Research on Influenza, London, UK. (gla.ac.uk)
Haemagglutination inhibition (HI) assays performed on dates between 1990 and 2021 by the WHO Collaborating Centre for Reference and Research on Influenza, London, UK on viruses collected between 1961 and 2021. (gla.ac.uk)
Furthermore, cross hemagglutination inhibition and cross virus neutralization assays, as well as animal experiments were conducted to determine the antigenicity and pathogenicity of those viruses. (biomedcentral.com)
The R value was calculated based on cross hemagglutination inhibition and cross virus neutralization results, and confirmed antigenic difference of the PPMV-1 strains isolated in 2013 from the LaSota vaccine strain. (biomedcentral.com)
Using a panel of antisera raised against H5N1 influenza viruses, the assay based on GFP expressing viruses was compared with the classical virus neutralization assay and the hemagglutination inhibition assay. (eur.nl)
The type of influenza virus (A or B) may be determined by immunofluorescence or hemagglutination inhibition (HAI) techniques, and the hemagglutinin subtypes of influenza A virus (H1, H2, H3) may be identified using HAI with subtype-specific antisera. (medscape.com)
By using a standard amount of virus, a standard amount of blood cells, and serially diluting the antiserum, one can identify the concentration of the antiserum (the greatest dilution which inhibits hemagglutination). (wikipedia.org)
Rabbit2
Significant cross-reactions in the horse antisera were compared to similar data obtained from rabbit antisera. (nih.gov)
For example, rabbit hyperimmune polyclonal antisera or human convalescent sera reacted with * 77 to 99% of two-dimensional electrophoresed proteins expressed by 14 M. hominis strains (1, 9), whereas MAbs raised against the type strain PG21 reacted with only a few of the other strains tested (13). (docksci.com)
Tests2
Hemagglutination tests were used at doctor's offices to diagnose pregnancy in the 1960s and 1970s. (nih.gov)
Tests that are dependent on the clumping of cells, microorganisms, or particles when mixed with specific antiserum. (nih.gov)
Direct2
ABO blood grouping was performed by direct haemagglutination technique using monoclonal antisera. (esmed.org)
11. Nishi K, Mizumoto J, Wada K, Tsuji T, Kimura A. Reliability of blood grouping of aged blood by two direct haemagglutination methods and absorption-elution methods. (esmed.org)
Cells1
Distilled water and urine are added to a freeze-dried mixture of hCG antiserum from rabbits and hCG-coated red blood cells from sheep. (nih.gov)
Include1
citation needed] This assay may be modified to include the addition of an antiserum. (wikipedia.org)